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Gene Dmel\CG18749

General Information
Symbol	Dmel\CG18749	Species	D. melanogaster
Name		Annotation symbol	CG18749
Feature type	protein_coding_gene	FlyBase ID	FBgn0042182
Gene Model Status	Current	Stock availability	3 publicly available
Also Known As	CG31188
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	84F5-84F6	Sequence location	3R:4,069,777..4,073,577 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	This gene is referred to in FlyBase by the symbol Dmel\CG18749 (FBgn0042182). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: procollagen-proline 4-dioxygenase activity. Based on sequence similarity, it is predicted to be involved in the biological process: peptidyl-proline hydroxylation to 4-hydroxy-L-proline. 3 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Oxoglutarate/iron-dependent dioxygenase; Prolyl 4-hydroxylase alpha-subunit, N-terminal; Prolyl 4-hydroxylase, alpha subunit. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages. This gene is annotated by FlyBase as a dicistronic gene, meaning that some or all of its transcripts encode two or more polypeptide-coding open reading frames (ORFs) , with each ORF assigned to a different gene. The distribution of RNA-Seq coverage data amongst the different encoded genes cannot be determined. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult male accessory gland. Comments on Affy2 ProbeSet: ProbeSet 1636894_s_at aligns to a genomic region shared by exons of 2 FlyBase-annotated genes; hence it is impossible to interpret the expression pattern and levels of CG18749 expression signal. See the GBrowse view of CG18749 to determine the overlapping gene(s). Gene sequence location is 3R:4069777..4073577.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03	Controlled Vocabulary Terms multicellular organism reproduction extracellular region
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 84F5-84F6   Limits computationally determined from genome sequence between P{PZ}grn05930 and P{PZ}Mcm2rL074
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\CG18749 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	gene_with_dicistronic_mRNA ; SO:0000722 Gene model reviewed during 5.49 Dicistronic structure supported by FBrf0155317.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) CG18749-RB FBtr0100545  NM_001031983   3404   491
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein CG18749-PB   FBpp0099996   55.8   491   7.08     NP_001027154   AAN14309
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Oxoglutarate/iron-dependent dioxygenase (IPR005123) Prolyl 4-hydroxylase, alpha subunit (IPR006620) Prolyl 4-hydroxylase alpha-subunit, N-terminal (IPR013547)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA246235   AA695536   AA802019   AE014297 AAN14309   AI062764   AI062838   AI107901   AI402026   AI517558   AI517568   AI532494   AI533941   AI534947   AY118744 AAM50604   BG633729   BI355037   BI355425   BI632162   BI637138   BI638234   BI640219   BT032938   CO178356   CO265055   CO322669   CO328489   CO337173   CO344833   DN154350   EC061305   EC209780   EC223537   EC246504   EC256354   EC264274   GH827572
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q8MSK0
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult male accessory gland. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    16.4 Larval Midgut    7.7 Larval Hindgut    8.5 Larval Malpighian Tubules    8.4 Larval Fat Body    9.7 Larval Salivary Gland    3.5 Larval Trachea    10.425 Larval Carcass    5.225 Adult Head    8.1 Adult Eye    10.55 Adult Brain    40.1 Adult Thoracic-Abdominal Ganglion    31.2 Adult Crop    8.7 Adult Midgut    6.2 Adult Hindgut    8.2 Adult Malpighian Tubules    4.6 Adult Fat Body    14 Adult Salivary Gland    7.7 Adult Heart    9.55 Adult VirginFemale Spermatheca    7.9 Adult InseminatedFemale Spermatheca    9 Adult Ovary    1.5 Adult Testis    24.2 Adult Male Accessory Gland    3523.3 Adult Carcass    7.8 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    16.4 Larval Midgut    7.7 Larval Hindgut    8.5 Larval Malpighian Tubules    8.4 Larval Fat Body    9.7 Larval Salivary Gland    3.5 Larval Trachea    10.425 Larval Carcass    5.225 Adult Head    8.1 Adult Eye    10.55 Adult Brain    40.1 Adult Thoracic-Abdominal Ganglion    31.2 Adult Crop    8.7 Adult Midgut    6.2 Adult Hindgut    8.2 Adult Malpighian Tubules    4.6 Adult Fat Body    14 Adult Salivary Gland    7.7 Adult Heart    9.55 Adult VirginFemale Spermatheca    7.9 Adult InseminatedFemale Spermatheca    9 Adult Ovary    1.5 Adult Testis    24.2 Adult Male Accessory Gland  (3523.3) Adult Carcass    7.8 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    16.4 Larval Midgut    7.7 Larval Hindgut    8.5 Larval Malpighian Tubules    8.4 Larval Fat Body    9.7 Larval Salivary Gland    3.5 Larval Trachea    10.425 Larval Carcass    5.225 Adult Head    8.1 Adult Eye    10.55 Adult Brain    40.1 Adult Thoracic-Abdominal Ganglion    31.2 Adult Crop    8.7 Adult Midgut    6.2 Adult Hindgut    8.2 Adult Malpighian Tubules    4.6 Adult Fat Body    14 Adult Salivary Gland    7.7 Adult Heart    9.55 Adult VirginFemale Spermatheca    7.9 Adult InseminatedFemale Spermatheca    9 Adult Ovary    1.5 Adult Testis    24.2 Adult Male Accessory Gland  (3523.3) Adult Carcass    7.8 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    16.4 Larval Midgut    7.7 Larval Hindgut    8.5 Larval Malpighian Tubules    8.4 Larval Fat Body    9.7 Larval Salivary Gland    3.5 Larval Trachea    10.425 Larval Carcass    5.225 Adult Head    8.1 Adult Eye    10.55 Adult Brain    40.1 Adult Thoracic-Abdominal Ganglion    31.2 Adult Crop    8.7 Adult Midgut    6.2 Adult Hindgut    8.2 Adult Malpighian Tubules    4.6 Adult Fat Body    14 Adult Salivary Gland    7.7 Adult Heart    9.55 Adult VirginFemale Spermatheca    7.9 Adult InseminatedFemale Spermatheca    9 Adult Ovary    1.5 Adult Testis    24.2 Adult Male Accessory Gland    3523.3 Adult Carcass    7.8 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    16.4 Larval Midgut    7.7 Larval Hindgut    8.5 Larval Malpighian Tubules    8.4 Larval Fat Body    9.7 Larval Salivary Gland    3.5 Larval Trachea    10.425 Larval Carcass    5.225 Adult Head    8.1 Adult Eye    10.55 Adult Brain    40.1 Adult Thoracic-Abdominal Ganglion    31.2 Adult Crop    8.7 Adult Midgut    6.2 Adult Hindgut    8.2 Adult Malpighian Tubules    4.6 Adult Fat Body    14 Adult Salivary Gland    7.7 Adult Heart    9.55 Adult VirginFemale Spermatheca    7.9 Adult InseminatedFemale Spermatheca    9 Adult Ovary    1.5 Adult Testis    24.2 Adult Male Accessory Gland    3523.3 Adult Carcass    7.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    16.4 Larval Midgut    7.7 Larval Hindgut    8.5 Larval Malpighian Tubules    8.4 Larval Fat Body    9.7 Larval Salivary Gland    3.5 Larval Trachea    10.425 Larval Carcass    5.225 Adult Head    8.1 Adult Eye    10.55 Adult Brain    40.1 Adult Thoracic-Abdominal Ganglion    31.2 Adult Crop    8.7 Adult Midgut    6.2 Adult Hindgut    8.2 Adult Malpighian Tubules    4.6 Adult Fat Body    14 Adult Salivary Gland    7.7 Adult Heart    9.55 Adult VirginFemale Spermatheca    7.9 Adult InseminatedFemale Spermatheca    9 Adult Ovary    1.5 Adult Testis    24.2 Adult Male Accessory Gland  (3523.3) Adult Carcass    7.8 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    16.4 Larval Midgut    7.7 Larval Hindgut    8.5 Larval Malpighian Tubules    8.4 Larval Fat Body    9.7 Larval Salivary Gland    3.5 Larval Trachea    10.425 Larval Carcass    5.225 Adult Head    8.1 Adult Eye    10.55 Adult Brain    40.1 Adult Thoracic-Abdominal Ganglion    31.2 Adult Crop    8.7 Adult Midgut    6.2 Adult Hindgut    8.2 Adult Malpighian Tubules    4.6 Adult Fat Body    14 Adult Salivary Gland    7.7 Adult Heart    9.55 Adult VirginFemale Spermatheca    7.9 Adult InseminatedFemale Spermatheca    9 Adult Ovary    1.5 Adult Testis    24.2 Adult Male Accessory Gland  (3523.3) Adult Carcass    7.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    16.4 Larval Midgut    7.7 Larval Hindgut    8.5 Larval Malpighian Tubules    8.4 Larval Fat Body    9.7 Larval Salivary Gland    3.5 Larval Trachea    10.425 Larval Carcass    5.225 Adult Head    8.1 Adult Eye    10.55 Adult Brain    40.1 Adult Thoracic-Abdominal Ganglion    31.2 Adult Crop    8.7 Adult Midgut    6.2 Adult Hindgut    8.2 Adult Malpighian Tubules    4.6 Adult Fat Body    14 Adult Salivary Gland    7.7 Adult Heart    9.55 Adult VirginFemale Spermatheca    7.9 Adult InseminatedFemale Spermatheca    9 Adult Ovary    1.5 Adult Testis    24.2 Adult Male Accessory Gland    3523.3 Adult Carcass    7.8 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    8.1   NA  Eye    no informative data   NA  Brain    40.1   16.4  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    31.2   NA  Crop    8.7   7.7  Midgut    6.2   8.5  Hindgut    8.2   8.4  Malpighian Tubules    4.6   9.7  Fat Body    no informative data   3.5  Salivary Gland    7.7   NA  Heart    9.55   10.425  Trachea    NA   NA  VirginFemale Spermatheca    7.9   NA  InseminatedFemale Spermatheca    9   NA  Ovary    1.5   NA  Testis    24.2   NA  Male Accessory Gland    3523.3   5.225  Carcass    7.8 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    25 central nervous system, larvae L3    23 central nervous system, pupae P8    16 head, virgin 1-day female    4 head, virgin 4-day female    5 head, virgin 20-day female    5 head, mated 1-day female    4 head, mated 4-day female    5 head, mated 20-day female    5 head, mated 1-day male    5 head, mated 4-day male    9 head, mated 20-day male    10 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    16 digestive system, 1-day adult    7 digestive system, 4-day adult    7 digestive system, 20-day adult    5 fat body, larvae L3 wandering    4 fat body, white prepupae    11 fat body, pupae P8    18 carcass, larvae L3 wandering    18 carcass, 1-day adult    4 carcass, 4-day adult    6 carcass, 20-day adult    6 ovary, virgin 4-day female    25 ovary, mated 4-day female    18 testis, mated 4-day male    58 accessory gland, mated 4-day male    82 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    25 central nervous system, larvae L3    23 central nervous system, pupae P8    16 head, virgin 1-day female    4 head, virgin 4-day female    5 head, virgin 20-day female    5 head, mated 1-day female    4 head, mated 4-day female    5 head, mated 20-day female    5 head, mated 1-day male    5 head, mated 4-day male    9 head, mated 20-day male    10 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    16 digestive system, 1-day adult    7 digestive system, 4-day adult    7 digestive system, 20-day adult    5 fat body, larvae L3 wandering    4 fat body, white prepupae    11 fat body, pupae P8    18 carcass, larvae L3 wandering    18 carcass, 1-day adult    4 carcass, 4-day adult    6 carcass, 20-day adult    6 ovary, virgin 4-day female    25 ovary, mated 4-day female    18 testis, mated 4-day male  (58) accessory gland, mated 4-day male  (82) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    25 central nervous system, larvae L3    23 central nervous system, pupae P8    16 head, virgin 1-day female    4 head, virgin 4-day female    5 head, virgin 20-day female    5 head, mated 1-day female    4 head, mated 4-day female    5 head, mated 20-day female    5 head, mated 1-day male    5 head, mated 4-day male    9 head, mated 20-day male    10 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    16 digestive system, 1-day adult    7 digestive system, 4-day adult    7 digestive system, 20-day adult    5 fat body, larvae L3 wandering    4 fat body, white prepupae    11 fat body, pupae P8    18 carcass, larvae L3 wandering    18 carcass, 1-day adult    4 carcass, 4-day adult    6 carcass, 20-day adult    6 ovary, virgin 4-day female    25 ovary, mated 4-day female    18 testis, mated 4-day male    58 accessory gland, mated 4-day male    82 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    25 central nervous system, larvae L3    23 central nervous system, pupae P8    16 head, virgin 1-day female    4 head, virgin 4-day female    5 head, virgin 20-day female    5 head, mated 1-day female    4 head, mated 4-day female    5 head, mated 20-day female    5 head, mated 1-day male    5 head, mated 4-day male    9 head, mated 20-day male    10 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    16 digestive system, 1-day adult    7 digestive system, 4-day adult    7 digestive system, 20-day adult    5 fat body, larvae L3 wandering    4 fat body, white prepupae    11 fat body, pupae P8    18 carcass, larvae L3 wandering    18 carcass, 1-day adult    4 carcass, 4-day adult    6 carcass, 20-day adult    6 ovary, virgin 4-day female    25 ovary, mated 4-day female    18 testis, mated 4-day male    58 accessory gland, mated 4-day male    82 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    25 central nervous system, larvae L3    23 central nervous system, pupae P8    16 head, virgin 1-day female    4 head, virgin 4-day female    5 head, virgin 20-day female    5 head, mated 1-day female    4 head, mated 4-day female    5 head, mated 20-day female    5 head, mated 1-day male    5 head, mated 4-day male    9 head, mated 20-day male    10 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    16 digestive system, 1-day adult    7 digestive system, 4-day adult    7 digestive system, 20-day adult    5 fat body, larvae L3 wandering    4 fat body, white prepupae    11 fat body, pupae P8    18 carcass, larvae L3 wandering    18 carcass, 1-day adult    4 carcass, 4-day adult    6 carcass, 20-day adult    6 ovary, virgin 4-day female    25 ovary, mated 4-day female    18 testis, mated 4-day male    58 accessory gland, mated 4-day male    82 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    25 central nervous system, larvae L3    23 central nervous system, pupae P8    16 head, virgin 1-day female    4 head, virgin 4-day female    5 head, virgin 20-day female    5 head, mated 1-day female    4 head, mated 4-day female    5 head, mated 20-day female    5 head, mated 1-day male    5 head, mated 4-day male    9 head, mated 20-day male    10 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    16 digestive system, 1-day adult    7 digestive system, 4-day adult    7 digestive system, 20-day adult    5 fat body, larvae L3 wandering    4 fat body, white prepupae    11 fat body, pupae P8    18 carcass, larvae L3 wandering    18 carcass, 1-day adult    4 carcass, 4-day adult    6 carcass, 20-day adult    6 ovary, virgin 4-day female    25 ovary, mated 4-day female    18 testis, mated 4-day male  (58) accessory gland, mated 4-day male  (82) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    25 central nervous system, larvae L3    23 central nervous system, pupae P8    16 head, virgin 1-day female    4 head, virgin 4-day female    5 head, virgin 20-day female    5 head, mated 1-day female    4 head, mated 4-day female    5 head, mated 20-day female    5 head, mated 1-day male    5 head, mated 4-day male    9 head, mated 20-day male    10 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    16 digestive system, 1-day adult    7 digestive system, 4-day adult    7 digestive system, 20-day adult    5 fat body, larvae L3 wandering    4 fat body, white prepupae    11 fat body, pupae P8    18 carcass, larvae L3 wandering    18 carcass, 1-day adult    4 carcass, 4-day adult    6 carcass, 20-day adult    6 ovary, virgin 4-day female    25 ovary, mated 4-day female    18 testis, mated 4-day male    58 accessory gland, mated 4-day male    82 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    25 central nervous system, larvae L3    23 central nervous system, pupae P8    16 head, virgin 1-day female    4 head, virgin 4-day female    5 head, virgin 20-day female    5 head, mated 1-day female    4 head, mated 4-day female    5 head, mated 20-day female    5 head, mated 1-day male    5 head, mated 4-day male    9 head, mated 20-day male    10 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    16 digestive system, 1-day adult    7 digestive system, 4-day adult    7 digestive system, 20-day adult    5 fat body, larvae L3 wandering    4 fat body, white prepupae    11 fat body, pupae P8    18 carcass, larvae L3 wandering    18 carcass, 1-day adult    4 carcass, 4-day adult    6 carcass, 20-day adult    6 ovary, virgin 4-day female    25 ovary, mated 4-day female    18 testis, mated 4-day male    58 accessory gland, mated 4-day male    82 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages. This gene is annotated by FlyBase as a dicistronic gene, meaning that some or all of its transcripts encode two or more polypeptide-coding open reading frames (ORFs) , with each ORF assigned to a different gene. The distribution of RNA-Seq coverage data amongst the different encoded genes cannot be determined. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    26 embryo 02-04hr    7 embryo 04-06hr    6 embryo 06-08hr    6 embryo 08-10hr    5 embryo 10-12hr    4 embryo 12-14hr    4 embryo 14-16hr    4 embryo 16-18hr    3 embryo 18-20hr    2 embryo 20-22hr    1 embryo 22-24hr    2 larva L1    6 larva L2    10 larva L3 12hr old    7 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    17 white prepupae new    18 white prepupae 12hr    12 white prepupae 24hr    15 pupae 2d postWPP    12 pupae 3d postWPP    7 pupae 4d postWPP    8 adult male 01day    12 adult male 05day    16 adult male 30day    14 adult female 01day    10 adult female 05day    19 adult female 30day    19 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  26 embryo 02-04hr    7 embryo 04-06hr    6 embryo 06-08hr    6 embryo 08-10hr    5 embryo 10-12hr    4 embryo 12-14hr    4 embryo 14-16hr    4 embryo 16-18hr    3 embryo 18-20hr    2 embryo 20-22hr    1 embryo 22-24hr    2 larva L1    6 larva L2    10 larva L3 12hr old    7 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    17 white prepupae new    18 white prepupae 12hr    12 white prepupae 24hr    15 pupae 2d postWPP    12 pupae 3d postWPP    7 pupae 4d postWPP    8 adult male 01day    12 adult male 05day    16 adult male 30day    14 adult female 01day    10 adult female 05day    19 adult female 30day    19 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    26 embryo 02-04hr    7 embryo 04-06hr    6 embryo 06-08hr    6 embryo 08-10hr    5 embryo 10-12hr    4 embryo 12-14hr    4 embryo 14-16hr    4 embryo 16-18hr    3 embryo 18-20hr    2 embryo 20-22hr    1 embryo 22-24hr    2 larva L1    6 larva L2    10 larva L3 12hr old    7 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    17 white prepupae new    18 white prepupae 12hr    12 white prepupae 24hr    15 pupae 2d postWPP    12 pupae 3d postWPP    7 pupae 4d postWPP    8 adult male 01day    12 adult male 05day    16 adult male 30day    14 adult female 01day    10 adult female 05day    19 adult female 30day    19 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    26 embryo 02-04hr    7 embryo 04-06hr    6 embryo 06-08hr    6 embryo 08-10hr    5 embryo 10-12hr    4 embryo 12-14hr    4 embryo 14-16hr    4 embryo 16-18hr    3 embryo 18-20hr    2 embryo 20-22hr    1 embryo 22-24hr    2 larva L1    6 larva L2    10 larva L3 12hr old    7 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    17 white prepupae new    18 white prepupae 12hr    12 white prepupae 24hr    15 pupae 2d postWPP    12 pupae 3d postWPP    7 pupae 4d postWPP    8 adult male 01day    12 adult male 05day    16 adult male 30day    14 adult female 01day    10 adult female 05day    19 adult female 30day    19 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    26 embryo 02-04hr    7 embryo 04-06hr    6 embryo 06-08hr    6 embryo 08-10hr    5 embryo 10-12hr    4 embryo 12-14hr    4 embryo 14-16hr    4 embryo 16-18hr    3 embryo 18-20hr    2 embryo 20-22hr    1 embryo 22-24hr    2 larva L1    6 larva L2    10 larva L3 12hr old    7 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    17 white prepupae new    18 white prepupae 12hr    12 white prepupae 24hr    15 pupae 2d postWPP    12 pupae 3d postWPP    7 pupae 4d postWPP    8 adult male 01day    12 adult male 05day    16 adult male 30day    14 adult female 01day    10 adult female 05day    19 adult female 30day    19 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  26 embryo 02-04hr    7 embryo 04-06hr    6 embryo 06-08hr    6 embryo 08-10hr    5 embryo 10-12hr    4 embryo 12-14hr    4 embryo 14-16hr    4 embryo 16-18hr    3 embryo 18-20hr    2 embryo 20-22hr    1 embryo 22-24hr    2 larva L1    6 larva L2    10 larva L3 12hr old    7 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    17 white prepupae new    18 white prepupae 12hr    12 white prepupae 24hr    15 pupae 2d postWPP    12 pupae 3d postWPP    7 pupae 4d postWPP    8 adult male 01day    12 adult male 05day    16 adult male 30day    14 adult female 01day    10 adult female 05day    19 adult female 30day    19 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    26 embryo 02-04hr    7 embryo 04-06hr    6 embryo 06-08hr    6 embryo 08-10hr    5 embryo 10-12hr    4 embryo 12-14hr    4 embryo 14-16hr    4 embryo 16-18hr    3 embryo 18-20hr    2 embryo 20-22hr    1 embryo 22-24hr    2 larva L1    6 larva L2    10 larva L3 12hr old    7 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    17 white prepupae new    18 white prepupae 12hr    12 white prepupae 24hr    15 pupae 2d postWPP    12 pupae 3d postWPP    7 pupae 4d postWPP    8 adult male 01day    12 adult male 05day    16 adult male 30day    14 adult female 01day    10 adult female 05day    19 adult female 30day    19 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    26 embryo 02-04hr    7 embryo 04-06hr    6 embryo 06-08hr    6 embryo 08-10hr    5 embryo 10-12hr    4 embryo 12-14hr    4 embryo 14-16hr    4 embryo 16-18hr    3 embryo 18-20hr    2 embryo 20-22hr    1 embryo 22-24hr    2 larva L1    6 larva L2    10 larva L3 12hr old    7 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    17 white prepupae new    18 white prepupae 12hr    12 white prepupae 24hr    15 pupae 2d postWPP    12 pupae 3d postWPP    7 pupae 4d postWPP    8 adult male 01day    12 adult male 05day    16 adult male 30day    14 adult female 01day    10 adult female 05day    19 adult female 30day    19 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    29 Schneider line 2 Sg4    20 embryonic 1182-4H    14 embryonic GM2    25 embryonic Kc167    21 embryonic S1    29 embryonic S3    25 leg disc CME L1    20 wing disc CME-W2    18 wing disc ML-DmD8    15 wing disc ML-DmD9    19 wing disc ML-DmD16-c3    19 wing disc ML-DmD21    14 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    20 eye-antennal disc ML-DmD11    28 antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    21 CNS ML-DmBG1-c1    20 CNS ML-DmBG2-c2    22 tumorous blood cells mbn2    24 ovary fGS/OSS    19 ovary OSC    26 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (29) Schneider line 2 Sg4    20 embryonic 1182-4H    14 embryonic GM2    25 embryonic Kc167    21 embryonic S1  (29) embryonic S3    25 leg disc CME L1    20 wing disc CME-W2    18 wing disc ML-DmD8    15 wing disc ML-DmD9    19 wing disc ML-DmD16-c3    19 wing disc ML-DmD21    14 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    20 eye-antennal disc ML-DmD11  (28) antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    21 CNS ML-DmBG1-c1    20 CNS ML-DmBG2-c2    22 tumorous blood cells mbn2    24 ovary fGS/OSS    19 ovary OSC  26 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    29 Schneider line 2 Sg4    20 embryonic 1182-4H    14 embryonic GM2    25 embryonic Kc167    21 embryonic S1    29 embryonic S3    25 leg disc CME L1    20 wing disc CME-W2    18 wing disc ML-DmD8    15 wing disc ML-DmD9    19 wing disc ML-DmD16-c3    19 wing disc ML-DmD21    14 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    20 eye-antennal disc ML-DmD11    28 antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    21 CNS ML-DmBG1-c1    20 CNS ML-DmBG2-c2    22 tumorous blood cells mbn2    24 ovary fGS/OSS    19 ovary OSC    26 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    29 Schneider line 2 Sg4    20 embryonic 1182-4H    14 embryonic GM2    25 embryonic Kc167    21 embryonic S1    29 embryonic S3    25 leg disc CME L1    20 wing disc CME-W2    18 wing disc ML-DmD8    15 wing disc ML-DmD9    19 wing disc ML-DmD16-c3    19 wing disc ML-DmD21    14 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    20 eye-antennal disc ML-DmD11    28 antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    21 CNS ML-DmBG1-c1    20 CNS ML-DmBG2-c2    22 tumorous blood cells mbn2    24 ovary fGS/OSS    19 ovary OSC    26 ovary OSS    8 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    29 Schneider line 2 Sg4    20 embryonic 1182-4H    14 embryonic GM2    25 embryonic Kc167    21 embryonic S1    29 embryonic S3    25 leg disc CME L1    20 wing disc CME-W2    18 wing disc ML-DmD8    15 wing disc ML-DmD9    19 wing disc ML-DmD16-c3    19 wing disc ML-DmD21    14 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    20 eye-antennal disc ML-DmD11    28 antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    21 CNS ML-DmBG1-c1    20 CNS ML-DmBG2-c2    22 tumorous blood cells mbn2    24 ovary fGS/OSS    19 ovary OSC    26 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  29 Schneider line 2 Sg4    20 embryonic 1182-4H    14 embryonic GM2    25 embryonic Kc167    21 embryonic S1  29 embryonic S3    25 leg disc CME L1    20 wing disc CME-W2    18 wing disc ML-DmD8    15 wing disc ML-DmD9    19 wing disc ML-DmD16-c3    19 wing disc ML-DmD21    14 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    20 eye-antennal disc ML-DmD11  28 antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    21 CNS ML-DmBG1-c1    20 CNS ML-DmBG2-c2    22 tumorous blood cells mbn2    24 ovary fGS/OSS    19 ovary OSC  26 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    29 Schneider line 2 Sg4    20 embryonic 1182-4H    14 embryonic GM2    25 embryonic Kc167    21 embryonic S1    29 embryonic S3    25 leg disc CME L1    20 wing disc CME-W2    18 wing disc ML-DmD8    15 wing disc ML-DmD9    19 wing disc ML-DmD16-c3    19 wing disc ML-DmD21    14 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    20 eye-antennal disc ML-DmD11    28 antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    21 CNS ML-DmBG1-c1    20 CNS ML-DmBG2-c2    22 tumorous blood cells mbn2    24 ovary fGS/OSS    19 ovary OSC    26 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    29 Schneider line 2 Sg4    20 embryonic 1182-4H    14 embryonic GM2    25 embryonic Kc167    21 embryonic S1    29 embryonic S3    25 leg disc CME L1    20 wing disc CME-W2    18 wing disc ML-DmD8    15 wing disc ML-DmD9    19 wing disc ML-DmD16-c3    19 wing disc ML-DmD21    14 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    20 eye-antennal disc ML-DmD11    28 antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    21 CNS ML-DmBG1-c1    20 CNS ML-DmBG2-c2    22 tumorous blood cells mbn2    24 ovary fGS/OSS    19 ovary OSC    26 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    4 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    13 Cadmium 50 mM 48 hrs, 4-day adult    13 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    11 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    12 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    16 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    18 Rotenone 2 μg 12 hrs, larvae L3    12 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    4 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    13 Cadmium 50 mM 48 hrs, 4-day adult    13 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    11 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    12 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    16 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    18 Rotenone 2 μg 12 hrs, larvae L3    12 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    4 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    13 Cadmium 50 mM 48 hrs, 4-day adult    13 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    11 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    12 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    16 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    18 Rotenone 2 μg 12 hrs, larvae L3    12 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    4 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    13 Cadmium 50 mM 48 hrs, 4-day adult    13 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    11 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    12 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    16 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    18 Rotenone 2 μg 12 hrs, larvae L3    12 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    4 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    13 Cadmium 50 mM 48 hrs, 4-day adult    13 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    11 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    12 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    16 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    18 Rotenone 2 μg 12 hrs, larvae L3    12 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    4 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    13 Cadmium 50 mM 48 hrs, 4-day adult    13 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    11 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    12 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    16 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    18 Rotenone 2 μg 12 hrs, larvae L3    12 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    4 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    13 Cadmium 50 mM 48 hrs, 4-day adult    13 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    11 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    12 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    16 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    18 Rotenone 2 μg 12 hrs, larvae L3    12 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    4 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    13 Cadmium 50 mM 48 hrs, 4-day adult    13 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    11 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    12 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    16 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    18 Rotenone 2 μg 12 hrs, larvae L3    12 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (Graveley et al., 2011) mE1_20_mRNA_expression_cluster_08
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0042182 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG31188-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable CG18749e03518 Sterility Allele fertile CG18749e03518 Phenotype manifest in Allele
Classical Alleles ( 2 )
For All Classical Alleles Show
Allele of CG18749 Class Mutagen Stocks Known lesion CG18749e03518 piggyBac transposase 2 Yes CG18749c00110 1 --
Alleles Carried on Transgenic Constructs ( 1 )
For All Alleles Carried on Transgenic Constructs Show
Allele of CG18749 Class Mutagen Stocks Known lesion CG18749NIG.18749R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD4763} NA P{GD17555} NA P{GD17560} NA P{NIG.18749R} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{PB}CG18749c00110 NA PBac{RB}CG18749e03518 NA
Gene Ontology: Function, Process & Cellular Component ( 10 unique terms )
Terms Based on Experimental Evidence ( 1 term )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
multicellular organism reproduction inferred from expression pattern (Ravi Ram and Wolfner, 2007)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 9 terms )
Molecular Function
CV term Evidence References
iron ion binding inferred from electronic annotation with InterPro:IPR006620 (FlyBase Curators et al., 2004-) L-ascorbic acid binding inferred from electronic annotation with InterPro:IPR006620 (FlyBase Curators et al., 2004-) oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen inferred from electronic annotation with InterPro:IPR013547 (FlyBase Curators et al., 2004-) procollagen-proline 4-dioxygenase activity inferred from sequence or structural similarity with mouse P4ha1 (FlyBase Genome Annotators, 2002-2003)
Biological Process
CV term Evidence References
oxidation-reduction process inferred from electronic annotation with InterPro:IPR005123, InterPro:IPR006620, InterPro:IPR013547 (FlyBase Curators et al., 2004-) peptidyl-proline hydroxylation to 4-hydroxy-L-proline inferred from sequence or structural similarity with mouse P4ha1 (FlyBase Genome Annotators, 2002-2003)
Cellular Component
CV term Evidence References
endoplasmic reticulum inferred from electronic annotation with InterPro:IPR013547 (FlyBase Curators et al., 2004-) extracellular region inferred from sequence model (Ravi Ram and Wolfner, 2007) procollagen-proline 4-dioxygenase complex inferred by curator from GO:0004656|GO:0018401 (FlyBase Genome Annotators, 2002-2003)
Sequence Ontology: Class of Gene
	gene_with_dicistronic_mRNA (FlyBase Genome Annotators, 2002-2003) nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions CG18749 allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0042182
Orthologs
OrthoDB Orthologs (13) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG61RQDS Dipteran inclusive ortholog search No orthologs identified Insect inclusive ortholog search No orthologs identified Arthropod inclusive ortholog search No orthologs identified Metazoa inclusive ortholog search No orthologs identified
Orthologs in Drosophila Species (EOG61RQDS)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\CG15864     Drosophila melanogaster fruit fly  Dmel\CG18749     Drosophila simulans   Dsim\GD18523   Y Drosophila simulans   Dsim\GD18524   Y Drosophila sechellia   Dsec\GM23710   Y Drosophila sechellia   Dsec\GM23711   Y Drosophila erecta   Dere\GG23922   Y Drosophila yakuba   Dyak\GE25857   Y Drosophila ananassae   Dana\GF23245   Y Drosophila pseudoobscura pseudoobscura   Dpse\GA13990   Y Drosophila persimilis   Dper\GL24185   Y Drosophila virilis   Dvir\GJ23004   Y Drosophila mojavensis   Dmoj\GI10646   Y
Orthologs in non-Drosophila Dipterans (None identified)
No non-Drosophilid orthologies identified
Orthologs in non-Dipteran Insects (None identified)
No non-Dipteran orthologies identified
Orthologs in non-Insect Arthropods (None identified)
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (0) based on analysis using Dmel annotation version 4.3
No orthologs identified
Stocks & Reagents
Stocks Listed in FlyBase ( 3 )
Bloomington	18163 w1118; PBac{RB}CG18749e03518 CG33722e03518
Harvard	- PBac{PB}CG18749c00110 - PBac{RB}CG18749e03518
Genomic Clones ( 1 )
	BACR45A07 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 28 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH05783
Other clones	IP20455
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT29441 GH02084 GH02184 GH28563 GH28574 GM02993 GM03732 GM29284 GM30720 LD06026 SD01242 SD04031 SD05983 SD19060 SD20468 SD23030 SD25777
Other clones	562243 EK056509 EK141412 EK188617 EK222407 EN13955 EP09306 EP19956 RP003319466
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0042182 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 3771984
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments	Dicistronic annotation CG31188 split out into separate annotations for each open reading frame, CG33722 and CG18749, in release 4.2 of the genome annotation. (FlyBase Genome Annotators, 2005) One or more of the processed transcripts for this gene contain(s) two non-overlapping open reading frames (ORFs). The non-overlapping ORFs are represented by CG33722 and CG18749. (FlyBase Genome Annotators, 2002-2003)
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_001031983 RefSeq (Proteins) • NP_001027154 DDBJ / EMBL / GenBank • AAG22134.2 AAM50604 AAN14309 AAN14309.1 AAO41516.1 AAO41517.1 AI107901 AI402026 AY118744 Entrez Gene - A searchable database of RefSeq genes. • 14 3771984 UniProtKB/TrEMBL • Q8MSK0
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Oxoglutarate/iron-dependent dioxygenase (IPR005123) Prolyl 4-hydroxylase, alpha subunit (IPR006620) Prolyl 4-hydroxylase alpha-subunit, N-terminal (IPR013547)
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0042182 DRSC - Results from RNAi screens. • FBgn0042182 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0042182 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG31188-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0042182 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 3771984 modMine - Data generated by the modENCODE project. • FBgn0042182
Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	CG18238   CG18749 (Ravi Ram and Wolfner, 2007, Rohrbough et al., 2007, Japanese National Institute of Genetics, 2012.5.21) CG31188 (Misra et al., 2002, Giot et al., 2003, )
Name Synonym
Secondary FlyBase IDs
	FBgn0037557 FBgn0051188 FBpp0081295 FBpp0081296 FBpp0081297 FBpp0081298 FBtr0081799 FBtr0081800 FBtr0081801 FBtr0081802
References ( 15 )
Research paper	Rohrbough et al., 2007, Genes Dev. 21(20): 2607--2628 Presynaptic establishment of the synaptic cleft extracellular matrix is required for post-synaptic differentiation. [FBrf0200665] Misra et al., 2002, Genome Biol. 3(12): RESEARCH0083 Annotation of the Drosophila melanogaster euchromatic genome: a systematic review. [FBrf0155827]
Supplementary material	Graveley et al., 2011, Nature 471(7339): Supplementary Table 33. Fuzzy Cluster Members. [FBrf0213980] Ravi Ram and Wolfner, 2007, Integr. Comp. Biol. 47(3): Supplementary Material. [FBrf0219931] Giot et al., 2003, Science 302(5651): Tables S1 to S7. [FBrf0166452]
Personal communication to FlyBase	Bloomington Drosophila Stock Center, 2004.11.6, Exelixis insertion alleles that are not in FlyBase. Exelixis insertion alleles that are not in FlyBase. [FBrf0179797] FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215] Rushton, 2002.3.12, FlyBase error report for CG15864 on Tue Mar 12 15:25:04 2002. FlyBase error report for CG15864 on Tue Mar 12 15:25:04 2002. [FBrf0155317] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file) Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078]
FlyBase analysis	FlyBase Curators, 2008, Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. [FBrf0202955] FlyBase Genome Annotators, 2005, Changes affecting gene model number or type in release 4.2 of the annotated D.melanogaster genome. Changes affecting gene model number or type in release 4.2 of the annotated D.melanogaster genome. [FBrf0182792] FlyBase Genome Annotators, 2002-2003, Changes affecting gene model number or type in releases 3.0 and 3.1 of the annotated D.melanogaster genome. Changes affecting gene model number or type in releases 3.0 and 3.1 of the annotated D.melanogaster genome. [FBrf0148886] FlyBase, 1996-, FlyBase inference based on genome sequence analysis. FlyBase inference based on genome sequence analysis. [FBrf0104946] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]
Stock list	Japanese National Institute of Genetics, 2012.5.21, RNAi stocks available from NIG-FLY. RNAi stocks available from NIG-FLY. [FBrf0218457]