FlyBase Gene Report: Dmel\Adgf-A2

General Information

Symbol

Dmel\Adgf-A2

Species

D. melanogaster

Name

Adenosine deaminase-related growth factor A2

Annotation Symbol

CG32178

Feature Type

protein_coding_gene

FlyBase ID

FBgn0043025

Gene Model Status

Current

Stock Availability

4 publicly available

Enzyme Name (EC)

Adenosine deaminase (3.5.4.4)

Gene Summary

Adenosine deaminase-related growth factor A2 (Adgf-A2) encodes a growth factor involved in spermatid development. [Date last reviewed: 2019-09-12] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

MSI

Key Links

Genomic Location

Cytogenetic map

75A1-75A1

Sequence location

3L:17,754,936..17,764,158 [-]

Recombination map

3-45

RefSeq locus

NT_037436 REGION:17754936..17764158

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (7 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables adenosine deaminase activity

inferred from sequence or structural similarity with FLYBASE:Adgf-A; FB:FBgn0036752, FLYBASE:Adgf-D; FB:FBgn0038172

(Zurovec et al., 2002)

inferred from biological aspect of ancestor with PANTHER:PTN000150817

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in spermatid development

inferred from expression pattern

(Matsushita et al., 2000)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in adenosine catabolic process

inferred from sequence or structural similarity with FLYBASE:Adgf-A; FB:FBgn0036752, FLYBASE:Adgf-D; FB:FBgn0038172

(Zurovec et al., 2002)

inferred from biological aspect of ancestor with PANTHER:PTN000150817

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in inosine biosynthetic process

inferred from biological aspect of ancestor with PANTHER:PTN000150817

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in membrane

inferred from direct assay

(Matsushita et al., 2000)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in extracellular space

inferred from biological aspect of ancestor with PANTHER:PTN000150875

(assigned by GO_Central )

(Gaudet et al., 2011)

located_in integral component of membrane

inferred from sequence model

(Matsushita et al., 2000)

Gene Group (FlyBase)

ADENOSINE DEAMINASES

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC)

Experimental Evidence

Predictions / Assertions

Adenosine + H(2)O = inosine + NH(3) (3.5.4.4)

Summaries

Gene Snapshot

Adenosine deaminase-related growth factor A2 (Adgf-A2) encodes a growth factor involved in spermatid development. [Date last reviewed: 2019-09-12]

Gene Group (FlyBase)

ADENOSINE DEAMINASES -: Adenosine deaminases catalyze the deamination of adenosine and deoxyadenosine to inosine and deoxyinosine.

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Adgf-A2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.46

Shares 5' UTR with downstream gene.

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Adgf-A2-RA

FBtr0075192

NM_080281

1814

561

Adgf-A2-RB

FBtr0110846

NM_001043147

1800

542

Adgf-A2-RC

FBtr0110847

NM_001043148

1794

542

Additional Transcript Data and Comments

Reported size (kB)

1.792 (longest cDNA)

(Matsushita et al., 2000)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Adgf-A2-PA

FBpp0074956

65.3

561

6.51

NP_525020

AAN11698

Adgf-A2-PB

FBpp0110143

63.2

542

6.72

NP_001036612

ABI31259

Adgf-A2-PC

FBpp0110144

63.2

542

6.72

NP_001036613

ABI31260

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

542 aa isoforms: Adgf-A2-PB, Adgf-A2-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

561 (aa); 66 (kD observed)

(Matsushita et al., 2000)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Adgf-A2 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Msi_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

pupal stage

(Matsushita et al., 2000)

adult stage | male

testis

(Matsushita et al., 2000)

spermatogenesis & adult stage

spermatocyte

(Matsushita et al., 2000)

Additional Descriptive Data

Msi transcript is expressed in mature primary spermatocytes.

(Matsushita et al., 2000)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

spermatogenesis & adult stage

spermatozoon

(Matsushita et al., 2000)

spermatocyte

(Matsushita et al., 2000)

spermatid

(Matsushita et al., 2000)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

While Msi transcript is expressed in mature primary spermatocytes, Msi protein is expressed in later stages of spermiogenesis as well.

(Matsushita et al., 2000)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in membrane

inferred from direct assay

(Matsushita et al., 2000)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Adgf-A2 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Adgf-A2

View exonic expression by tissue for Dmel\Adgf-A2

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB

64879/tissue=All

EMBL-EBI Single Cell Expression Atlas

FBgn0043025

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG32178-RA

Flygut - An atlas of the Drosophila adult midgut

FBgn0043025

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 6 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Adgf-A2

Transgenic constructs containing regulatory region of Adgf-A2

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(3L)Exel9005

(Ryder, 2004.4.26)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Adgf-A2^GD2696

viable, with Scer\GAL4^Mef2.PR

Adgf-A2^GD2696

viable, with Scer\GAL4^pnr-MD237

Adgf-A2^GD2696

viable, with Scer\GAL4^tin.CΔ4

Adgf-A2^GD2696

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (6)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\ADA2

7 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (5)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Adal

1 of 15

Yes

Mmus\Ada

1 of 15

Yes

Mmus\Ampd1

1 of 15

Yes

Mmus\Ampd2

1 of 15

Yes

Mmus\Ampd3

1 of 15

Yes

Rattus norvegicus (Norway rat) (5)

Rnor\Adal

1 of 13

Yes

Rnor\Ada

1 of 13

Yes

Rnor\Ampd1

1 of 13

Yes

Rnor\Ampd2

1 of 13

Yes

Rnor\Ampd3

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (7)

Xtro\ada2

7 of 12

Yes

Xtro\ada.2

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Danio rerio (Zebrafish) (9)

Drer\ada2a

7 of 15

Yes

Drer\ada2b

7 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (3)

Cele\C44B7.12

2 of 15

Yes

Cele\ampd-1

1 of 15

Cele\C06G3.5

1 of 15

Arabidopsis thaliana (thale-cress) (1)

Atha\AT4G04880

2 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (4)

Scer\AAH1

3 of 15

Yes

Scer\AMD1

1 of 15

Scer\YBR284W

1 of 15

Scer\YJL070C

1 of 15

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\SPBC1683.02

1 of 12

Yes

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091905I5 )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA16735

Drosophila persimilis

Dper\GL25219

Drosophila willistoni

Dwil\GK12326

Drosophila virilis

Dvir\GJ13086

Drosophila mojavensis

Dmoj\GI12943

Drosophila grimshawi

Dgri\GH15339

Drosophila grimshawi

Dgri\GH17999

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( None identified )

No non-Drosophilid orthologies identified

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( None identified )

No non-Dipteran orthologies identified

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0873 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_307202

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0CQY )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Cecr1

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Cecr1L

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Cecr1_1

Gallus gallus

Domestic chicken

Ggal\CECR1

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (7)

Gene Symbol

Score

Source

Alignment

5 of 10

5 of 10

5 of 10

5 of 10

4 of 10

Ada

3 of 10

AMPdeam

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

ADA2; adenosine deaminase 2

model of Sneddon syndrome

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

ADA2; adenosine deaminase 2

7 of 15

607575

Sneddon syndrome

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0043025

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-45

Cytogenetic map

75A1-75A1

Sequence location

3L:17,754,936..17,764,158 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

75A1-75A1

Limits computationally determined from genome sequence between P{lacW}l(3)j11B2^j11B2 and P{PZ}W⁰⁵⁰¹⁴

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

75A-75A

(determined by in situ hybridisation)

(Matsushita et al., 2000)

Experimentally Determined Recombination Data

Location

3-45

(FlyBase, 2016)

3-41.2

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (4)

Bloomington

56044

y¹ sc^* v¹ sev²¹; P{TRiP.HMC04352}attP40

29268

w¹¹¹⁸; Mi{ET1}Adgf-A^MB11970 Adgf-A2^MB11970 CG32181^MB11970

VDRC

v8414

w¹¹¹⁸; P{GD2696}v8414

v8413

w¹¹¹⁸; P{GD2696}v8413/CyO

Genomic Clones (12)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (23)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

AT05468

Other clones

FI21284

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0043025

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0043025

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

64879

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Matsushita et al., 2000)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Adgf-A2 Msi

(FlyBase, 2009-)

Source for database merge of

Source for merge of: Msi BcDNA:AT05468

(FlyBase, 1996-)

Source for merge of: Msi CG32178

(FlyBase, 1996-)

Additional comments

Renamed from 'Msi' to Adgf-A2' to reflect usage in the literature and to make Adgf gene nomenclature consistent.

(FlyBase, 2009-)

Source for merge of Msi BcDNA:AT05468 was shared cDNA (AT05468) ( date:030529 ).

(FlyBase Genome Annotators, 2006, FlyBase, 1996-)

Source for merge of Msi CG32178 was sequence comparison ( date:021112 ).

(FlyBase, 1996-)

Other Comments

New annotation (CG32178) in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Msi exhibits growth factor activity when expressed in Schneider line 2 cells.

(Matsushita et al., 2000)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 43 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

64879

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones