FB2022_02, released March 29, 2022

Gene: Dmel\cbt

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General Information
Symbol
Dmel\cbt
Species
D. melanogaster
Name
cabut
Annotation Symbol
CG4427
Feature Type
protein_coding_gene
FlyBase ID
FBgn0043364
Gene Model Status
Current
Stock Availability
12 publicly available
Gene Summary
cabut (cbt) encodes a transcription factor that controls Dpp signaling and is involved in dorsal closure and wing disc morphogenesis. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)
All Summaries
Also Known As

EP2237, dTIEG

Key Links
Genomic Location
Cytogenetic map
21D1-21D1
Sequence location
Recombination map
2-1
RefSeq locus
NT_033779 REGION:476446..479688
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (11 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables sequence-specific DNA binding
inferred from direct assay
(Brown et al., 2005)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
enables DNA-binding transcription factor activity, RNA polymerase II-specific
inferred from biological aspect of ancestor with PANTHER:PTN004619861
(assigned by GO_Central )
(Gaudet et al., 2011)
inferred from sequence or structural similarity with HGNC:11811
(Munoz-Descalzo et al., 2007)
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding
inferred from biological aspect of ancestor with PANTHER:PTN004619861
(assigned by GO_Central )
(Gaudet et al., 2011)
Biological Process (7 terms)
Terms Based on Experimental Evidence (6 terms)
CV Term
Evidence
References
involved_in dorsal closure
inferred from mutant phenotype
(Munoz-Descalzo et al., 2005)
involved_in negative regulation of transcription by RNA polymerase II
inferred from direct assay
(Belacortu et al., 2012)
involved_in positive regulation of BMP signaling pathway
inferred from mutant phenotype
(Rodriguez, 2011)
involved_in sensory organ development
inferred from mutant phenotype
(Abdelilah-Seyfried et al., 2000)
involved_in wing disc dorsal/ventral pattern formation
inferred from genetic interaction with FLYBASE:Bx; FB:FBgn0265598, FLYBASE:ap; FB:FBgn0267978
(Bejarano et al., 2008)
involved_in wing disc morphogenesis
inferred from mutant phenotype
(Rodriguez, 2011)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in regulation of transcription by RNA polymerase II
inferred from biological aspect of ancestor with PANTHER:PTN004619861
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
located_in nucleus
inferred from direct assay
(Belacortu et al., 2012)
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Pathway (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
cabut (cbt) encodes a transcription factor that controls Dpp signaling and is involved in dorsal closure and wing disc morphogenesis. [Date last reviewed: 2019-03-07]
Gene Group (FlyBase)
SP1/KLF TRANSCRIPTION FACTORS -
The Sp1/KLF (Sp1-like/Kruppel-like factors) family of zinc finger proteins are DNA binding proteins. They can act as activators or repressors and some members bind sequences within Polycomb Response Elements (PREs). (Adapted from FBrf0188187).
C2H2 ZINC FINGER TRANSCRIPTION FACTORS -
Zinc finger C2H2 transcription factors are sequence-specific DNA binding proteins that regulate transcription. They possess DNA-binding domains that are formed from repeated Cys2His2 zinc finger motifs. (Adapted from PMID:1835093, FBrf0220103 and FBrf0155739).
Pathway (FlyBase)
Positive Regulators of BMP Signaling Pathway -
Positive regulators of Bone Morphogenetic Protein (BMP) signaling up-regulate the pathway, ultimately resulting in the increased nuclear activity of the Mad/Med transcription factor complex.
Summary (Interactive Fly)
Gene Model and Products
Number of Transcripts
3
Number of Unique Polypeptides
2

Please see the JBrowse view of Dmel\cbt for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure New Section
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9VPQ5)

If you don't see the viewer to the right, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.42

Gene model reviewed during 5.46

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
cbt-RA
FBtr0078083
NM_164384
2041
428
cbt-RB
FBtr0078084
NM_134685
3243
347
cbt-RC
FBtr0310030
NM_001272903
2361
347
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
cbt-PA
FBpp0077743
46.7
428
9.28
NP_722636
AAF51489
cbt-PB
FBpp0077744
37.9
347
9.63
NP_608529
AAN10498
cbt-PC
FBpp0301733
37.9
347
9.63
NP_001259832
AGB92369
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

347 aa isoforms: cbt-PB, cbt-PC
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\cbt using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 3 -- 5
organism | ubiquitous
(Belacortu et al., 2011)
embryonic stage 12 -- 14
embryonic epidermis | lateral
(Belacortu et al., 2011)
embryonic/larval midgut primordium
(Belacortu et al., 2011)
embryonic hindgut
(Belacortu et al., 2011)
embryonic stage 13 -- 14
amnioserosa
(Belacortu et al., 2011)
embryonic stage 14
yolk nucleus
(Belacortu et al., 2011)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
day 5 of adulthood & male -- day 7 of adulthood & male
spermatozoon
(Wasbrough et al., 2010)
Additional Descriptive Data

cbt protein is nuclear and is first detected in the germ plasm and pole cells of syncytial embryos, and subsequently, in blastoderm stages and during gastrulation. During germband retraction and dorsal closure it is localized in the yolk cell nuclei, lateral epidermis, and the gut primordium/hindgut. It is also present in the amnioserosa at these stages, despite the mRNA not being detected in this tissue.

(Belacortu et al., 2011)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in nucleus
inferred from direct assay
(Belacortu et al., 2012)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\cbt in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 13 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 12 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of cbt
Transgenic constructs containing regulatory region of cbt
Aberrations (Deficiencies and Duplications) ( 3 )
Inferred from experimentation ( 3 )
Gene disrupted in
Df(2L)cbtS14
(Rodriguez, 2011)
Df(2L)cbtS27
(Rodriguez, 2011)
Df(2L)cbtS161
(Rodriguez, 2011)
Inferred from location ( 0 )
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal, with Scer\GAL4Bx-MS1096
lethal (with Df(2L)BSC107)
lethal (with Df(2L)cbtS14)
lethal (with Df(2L)cbtS161)
lethal | cold sensitive, with Scer\GAL4Bx-MS1096
lethal - all die before end of P-stage
lethal - all die during embryonic stage
lethal - all die during embryonic stage | partially
partially lethal | conditional, with Scer\GAL4αTub84B.PL
viable
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
viable (with cbtD41)
viable (with cbtD148)
viable (with Df(2L)BSC107)
viable (with Df(2L)cbtS14)
viable (with Df(2L)cbtS161)
viable | recessive, with Scer\GAL4- :
Other Phenotypes
Allele
abnormal cell polarity | embryonic stage
abnormal circadian rhythm | adult stage, with Scer\GAL4tim.PE
abnormal developmental rate | conditional, with Scer\GAL4αTub84B.PL
abnormal locomotor behavior | adult stage, with Scer\GAL4tim.PE
abnormal neuroanatomy | larval stage, with Scer\GAL4elav-C155
abnormal neuroanatomy | pharate adult stage, with Scer\GAL4CCAP.PP
decreased occurrence of cell division | larval stage | conditional
decreased size | adult stage | somatic clone
decreased size | dominant | third instar larval stage
increased cell number, with Scer\GAL4salm.EPv
increased cell number | adult stage, with Scer\GAL4mex1.2.1
increased cell number | pupal stage P6 | somatic clone, with Scer\GAL4Tub.PU
increased occurrence of cell division | adult stage, with Scer\GAL4esg.PU
increased occurrence of cell division | pupal stage P6, with Scer\GAL4en.PU
increased occurrence of cell division | pupal stage P6, with Scer\GAL4GMR.PU
increased occurrence of cell division | pupal stage P6 | somatic clone, with Scer\GAL4Tub.PU
increased occurrence of cell division | third instar larval stage, with Scer\GAL4en.PU
majority die during first instar larval stage
some die during embryonic stage
some die during embryonic stage | partially
some die during P-stage
some die during second instar larval stage
some die during third instar larval stage
visible, with Scer\GAL4hh.PU
visible, with Scer\GAL4salm.EPv
visible, with Scer\GAL4sca-537.4
visible, with Scer\GAL4sd.PU
Phenotype manifest in
Allele
1st posterior cell, with Scer\GAL4hh.PU
adult midgut epithelium, with Scer\GAL4mex1.2.1
amnioserosa
anterior fascicle & synapse, with Scer\GAL4elav-C155
crossvein, with Scer\GAL4hh.PU
dorsocentral bristle, with Scer\GAL4sca-537.4
dorsocentral bristle | ectopic, with Scer\GAL4sca-537.4
embryo | dorsal closure stage
embryonic/first instar larval cuticle | anterior
embryonic/first instar larval cuticle | dorsal
embryonic leading edge cell
eye disc | pupal stage P6, with Scer\GAL4en.PU
eye disc | pupal stage P6 | somatic clone, with Scer\GAL4Tub.PU
gut epithelium | adult stage, with Scer\GAL4esg.PU
gut epithelium | adult stage | somatic clone
gut epithelium | adult stage | somatic clone, with Scer\GAL4esg.PU
intestinal stem cell | adult stage, with Scer\GAL4esg.PU
leading edge & filamentous actin
leading edge & muscle myosin complex
neurite | pharate adult stage, with Scer\GAL4CCAP.PP
ocellus, with Scer\GAL4sca-537.4
scutellar bristle, with Scer\GAL4sca-537.4
scutellar bristle | ectopic, with Scer\GAL4sca-537.4
tormogen cell, with Scer\GAL4sca-537.4
trichogen cell, with Scer\GAL4sca-537.4
trichogen cell | ectopic, with Scer\GAL4sca-537.4
wing, with Scer\GAL4salm.EPv
wing, with Scer\GAL4sd.PU
wing disc | pupal stage P6 | somatic clone, with Scer\GAL4Tub.PU
wing disc | third instar larval stage
wing disc posterior compartment, with Scer\GAL4hh.PU
wing disc posterior compartment | pupal stage P6, with Scer\GAL4en.PU
wing disc posterior compartment | third instar larval stage, with Scer\GAL4en.PU
wing margin, with Scer\GAL4sd.PU
wing pouch, with Scer\GAL4salm.EPv
wing vein, with Scer\GAL4salm.EPv
wing vein L5, with Scer\GAL4sd.PU
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (21)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\KLF11
6 of 15
Yes
Yes
Hsap\KLF10
2 of 15
No
Yes
Hsap\KLF13
2 of 15
No
Yes
Hsap\KLF15
2 of 15
No
No
1  
Hsap\EGR1
1 of 15
No
No
Hsap\EGR2
1 of 15
No
No
Hsap\EGR3
1 of 15
No
No
Hsap\EGR4
1 of 15
No
No
Hsap\KLF3
1 of 15
No
No
1  
Hsap\KLF4
1 of 15
No
No
Hsap\KLF8
1 of 15
No
No
Hsap\KLF9
1 of 15
No
Yes
Hsap\KLF12
1 of 15
No
No
1  
Hsap\KLF14
1 of 15
No
No
Hsap\KLF16
1 of 15
No
Yes
Hsap\OSR1
1 of 15
No
No
Hsap\OSR2
1 of 15
No
No
Hsap\SP2
1 of 15
No
No
Hsap\ZNF174
1 of 15
No
Yes
Hsap\ZNF362
1 of 15
No
No
Hsap\ZSCAN16
1 of 15
No
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (15)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Klf11
7 of 15
Yes
Yes
Mmus\Klf10
4 of 15
No
Yes
Mmus\Klf13
2 of 15
No
Yes
Mmus\Klf15
2 of 15
No
No
Mmus\Egr1
1 of 15
No
No
Mmus\Egr3
1 of 15
No
No
Mmus\Egr4
1 of 15
No
No
Mmus\Klf9
1 of 15
No
Yes
Mmus\Klf14
1 of 15
No
Yes
Mmus\Klf16
1 of 15
No
Yes
Mmus\Osr1
1 of 15
No
No
1  
Mmus\Osr2
1 of 15
No
No
Mmus\Sp2
1 of 15
No
No
Mmus\Zfp174
1 of 15
No
Yes
Mmus\Zfp362
1 of 15
No
No
Rattus norvegicus (Norway rat) (7)
Rnor\Klf10
4 of 13
Yes
Yes
Rnor\Klf16
2 of 13
No
Yes
Rnor\Klf9
1 of 13
No
Yes
Rnor\Klf11
1 of 13
No
Yes
Rnor\Klf14
1 of 13
No
No
Rnor\Klf15
1 of 13
No
No
Rnor\Zfp362
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (11)
Xtro\klf11
4 of 12
Yes
Yes
Xtro\klf13
2 of 12
No
Yes
Xtro\egr1
1 of 12
No
No
Xtro\egr2
1 of 12
No
No
Xtro\egr3
1 of 12
No
No
Xtro\klf9
1 of 12
No
Yes
Xtro\klf10
1 of 12
No
Yes
Xtro\klf15
1 of 12
No
No
Xtro\osr1
1 of 12
No
No
Xtro\osr2
1 of 12
No
No
Xtro\sp2
1 of 12
No
No
Danio rerio (Zebrafish) (17)
Drer\klf11a
7 of 15
Yes
Yes
Drer\klf11b
4 of 15
No
Yes
Drer\klf13
2 of 15
No
Yes
Drer\klf15
2 of 15
No
No
Drer\si:ch211-117k10.3
2 of 15
No
No
Drer\zgc:153115
2 of 15
No
Yes
Drer\egr1
1 of 15
No
No
Drer\egr2b
1 of 15
No
No
Drer\egr4
1 of 15
No
No
Drer\klf4
1 of 15
No
Yes
Drer\klf9
1 of 15
No
Yes
Drer\osr1
1 of 15
No
No
Drer\osr2
1 of 15
No
No
Drer\sp2
1 of 15
No
No
Drer\sp5l
1 of 15
No
No
Drer\znf281b
1 of 15
No
Yes
Drer\znf362b
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (6)
Cele\egrh-1
1 of 15
Yes
No
Cele\egrh-3
1 of 15
Yes
No
Cele\klu-1
1 of 15
Yes
No
Cele\odd-1
1 of 15
Yes
No
Cele\odd-2
1 of 15
Yes
No
Cele\sptf-2
1 of 15
Yes
No
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (9)
Scer\FZF1
3 of 15
Yes
No
Scer\ACE2
1 of 15
No
Yes
Scer\AZF1
1 of 15
No
No
Scer\CRZ1
1 of 15
No
No
Scer\MSN2
1 of 15
No
No
Scer\MSN4
1 of 15
No
No
Scer\RGM1
1 of 15
No
No
Scer\RPN4
1 of 15
No
Yes
Scer\SWI5
1 of 15
No
No
Schizosaccharomyces pombe (Fission yeast) (2)
Spom\hsr1
2 of 12
Yes
Yes
Spom\prz1
2 of 12
Yes
Yes
Other Organism Orthologs (via OrthoDB)
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (14)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
Klf15
3 of 10
luna
3 of 10
btd
2 of 10
CG42741
2 of 10
dar1
2 of 10
Sp1
2 of 10
sr
2 of 10
bowl
1 of 10
CG3065
1 of 10
hkb
1 of 10
ken
1 of 10
l(3)neo38
1 of 10
odd
1 of 10
Spps
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 1 )
    Human Ortholog
    Disease
    Evidence
    References
    KLF11; Kruppel like factor 11
    model of  maturity-onset diabetes of the young type 7
    IEA
    (FlyBase, 2019-)
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    KLF11; Kruppel like factor 11
    6 of 15
    603301
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement
    View in Interactions Browser
    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    cbt
    suppressible
    E2f1
    (Zhang et al., 2021)
    cbt
    suppressible
    stg
    (Zhang et al., 2021)
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Bx
    suppressible
    cbt
    (Bejarano et al., 2008)
    Bx|ap
    suppressible
    cbt
    (Bejarano et al., 2008)
    E2f1
    suppressible
    cbt
    (Zhang et al., 2021)
    hep
    suppressible
    cbt
    (Munoz-Descalzo et al., 2005)
    mir-958
    suppressible
    cbt
    (Mukherjee et al., 2021)
    pnr
    enhanceable
    cbt
    (Pena-Rangel et al., 2002)
    tkv
    suppressible
    cbt
    (Rodriguez, 2011)
    External Data
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Positive Regulators of BMP Signaling Pathway -
    Positive regulators of Bone Morphogenetic Protein (BMP) signaling up-regulate the pathway, ultimately resulting in the increased nuclear activity of the Mad/Med transcription factor complex.
    Metabolic Pathways
    FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
    External Data
    Linkouts
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2L
    Recombination map
    2-1
    Cytogenetic map
    21D1-21D1
    Sequence location
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    21D1-21D1
    Limits computationally determined from genome sequence between P{lacW}l(2)k09610k09610&P{lacW}Sk09538a and P{PZ}l(2)1068510685&P{lacW}Tango14k00619
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    21C4-21C6
    (determined by in situ hybridisation)
    (Abdelilah-Seyfried et al., 2000)
    Experimentally Determined Recombination Data
    Location

    2-1

    (FlyBase, 2016)

    2-0.0

    (Comeron et al., 2012)
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (12)
    Genomic Clones (15)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (51)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    BDGP DGC clones
    Other clones
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
     

    polyclonal

    (Belacortu et al., 2011)
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of

    Source for identity of: cabut EP2237

    (Munoz, 2004.2.25)
    Source for database merge of

    Source for merge of: EP2237 CG4427

    (Kraut et al., 2001, Kraut et al., 2001)
    Additional comments
    Other Comments

    dsRNA made from templates generated with primers directed against this gene results in a change in cell proliferation.

    (Guertin et al., 2006)

    Molecular and genetic analyses show that cbt expression is dependent on the JNK cascade during dorsal closure, and functions downstream of Jra to regulate dpp expression in the leading edge cells.

    (Munoz-Descalzo et al., 2005)

    cbt is essential for dorsal closure.

    (Munoz-Descalzo et al., 2004)

    Embryos mutant for cbt do not complete embryogenesis and frequently have holes in the dorsal-anterior region of their cuticles. The polarization of the dorsal-most epidermal cells in dorsal closure and also the activation of the JNK cascade at the leading edge cells is affected in cbt mutants.

    (Munoz-Descalzo et al., 2004)

    Identification: in a gain-of-function screen for genes involved in the generation of planar cell polarity.

    (Munoz-Descalzo et al., 2003)
    Origin and Etymology
    Discoverer
    Etymology
    Identification
    External Crossreferences and Linkouts ( 50 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
    EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    InterPro - A database of protein families, domains and functional sites
    KEGG Genes - Molecular building blocks of life in the genomic space.
    MARRVEL_MODEL - MARRVEL (model organism gene)
    modMine - A data warehouse for the modENCODE project
    SignaLink - A signaling pathway resource with multi-layered regulatory networks.
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
    FlyMine - An integrated database for Drosophila genomics
    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Synonyms and Secondary IDs (11)
    Reported As
    Symbol Synonym
    CG4427
    (Meadows, 2017.1.11, Ni et al., 2010.12.1, Fujikawa et al., 2009, Ida et al., 2009, Guertin et al., 2006, Molnar et al., 2006, Brown et al., 2005, Munoz-Descalzo et al., 2005, Rodriguez and Guerrero, 2005, Lim et al., 2004, Munoz, 2004.2.25, Wheeler et al., 2004)
    Cabot
    (Brown et al., 2005)
    Cabut
    (Kent and Agrawal, 2020, Chng et al., 2017, Ragheb et al., 2017, Beckstead et al., 2005)
    Cbt
    (Parra-Peralbo et al., 2021, Srivastava et al., 2021, Wang et al., 2021, Yaniv and Schuldiner, 2016)
    EP(2)2237
    (Pena-Rangel et al., 2002)
    EP2237
    (Chen et al., 2008, Yatsu et al., 2008, Kadener et al., 2007, Birch-Machin et al., 2005, Suske et al., 2005, Wheeler et al., 2004, Gorski et al., 2003, Zinke et al., 2002, Kraut et al., 2001, Kraut et al., 2001, Abdelilah-Seyfried et al., 2000, Zou et al., 2000)
    cabut
    (Chatterjee and Perrimon, 2021, Signor, 2020, Havula et al., 2013, Beckstead et al., 2005)
    cbt
    (Bargiela and Artero, 2021, Mukherjee et al., 2021, Yoo et al., 2021, Zhang et al., 2021, Shokri et al., 2019, Wilinski et al., 2019, Sarov et al., 2016, Baëza et al., 2015, Bartok et al., 2015, model organism Encyclopedia of Regulatory Network (modERN) Project, 2015-, Schertel et al., 2015, Luck et al., 2014, Carter, 2013, de Celis et al., 2013.9.11, Awofala et al., 2012, Zhou et al., 2012, Abruzzi et al., 2011, Belacortu et al., 2011, Rodriguez, 2011, Truscott et al., 2011, Blanco et al., 2010, Nagoshi et al., 2010, Wasbrough et al., 2010, Dworkin et al., 2009, Fujikawa et al., 2009, Ida et al., 2009, Bushati et al., 2008, Khokhar et al., 2008, Lee et al., 2008, Zhao et al., 2008, Belacortu et al., 2007, Isogai et al., 2007, Minidorff et al., 2007, Minidorff et al., 2007, Munoz-Descalzo et al., 2007)
    dTIEG
    (Zhang et al., 2021, Rodriguez, 2011, Rodriguez and Guerrero, 2005)
    Name Synonyms
    Cabut
    (Bartok et al., 2015, Belacortu et al., 2011)
    TGF-beta-inducible early gene
    (Rodriguez and Guerrero, 2005)
    cabot
    (Brown et al., 2005)
    cabut
    (Havula and Hietakangas, 2018, Signor and Nuzhdin, 2018, Mattila and Hietakangas, 2017, Belacortu et al., 2011, Rodriguez, 2011, Bülow et al., 2010, Lebo et al., 2009, Bejarano et al., 2008, Zhao et al., 2008, Belacortu et al., 2007, Molnar et al., 2006, Morozova et al., 2006)
    Secondary FlyBase IDs
    • FBgn0031259
    Datasets (8)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    Study result (8)
    Result
    Result Type
    Title
    scRNAseq_2020_Cattenoz_NI_seq_clustering
    Clustering analysis of hemocytes from non-infested third instar larvae
    scRNAseq_2020_Cattenoz_WI_seq_clustering
    Clustering analysis of hemocytes from wasp-infested third instar larvae
    scRNAseq_2020_Cho_NI72LG_seq_clustering
    Clustering analysis of lymph gland cells from non-infested larvae at 72 h after egg-laying
    scRNAseq_2020_Cho_NI96LG_seq_clustering
    Clustering analysis of lymph gland cells from non-infested larvae at 96 h after egg-laying
    scRNAseq_2020_Cho_NI120LG_seq_clustering
    Clustering analysis of lymph gland cells from non-infested larvae at 120 h after egg-laying
    scRNAseq_2020_Cho_WI96LG_seq_clustering
    Clustering analysis of lymph gland cells from wasp-infested larvae at 96 h after egg-laying
    scRNAseq_2020_Cho_NI96HL_seq_clustering
    Clustering analysis of circulating hemocytes from non-infested larvae at 96 h after egg-laying
    scRNAseq_2020_Cho_NI120HL_seq_clustering
    Clustering analysis of circulating hemocytes from non-infested larvae at 120 h after egg-laying
    References (123)