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Gene Dmel\slam

General Information
Symbol	Dmel\slam	Species	D. melanogaster
Name	slow as molasses	Annotation symbol	CG9506
Feature type	protein_coding_gene	FlyBase ID	FBgn0043854
Gene Model Status	Current	Stock availability	3 publicly available
Also Known As	wdo
Genomic Location
Chromosome (arm)	2L	Recombination map	2L-
Cytogenetic map	26C4-26C4	Sequence location	2L:6,372,415..6,377,287 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene slow as molasses is referred to in FlyBase by the symbol Dmel\slam (CG9506, FBgn0043854). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: germ cell migration; cellularization. 9 alleles are reported. The phenotypes of these alleles are annotated with: embryonic/larval midgut primordium; gonadal sheath proper primordium; germline cell; cycle 14 embryo. It has one annotated transcript and one annotated polypeptide. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of extremely low expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1638948_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of slam. Gene sequence location is 2L:6372415..6377287.
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2012_01	Sequence features HFA03240 BKN24518
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 26C4-26C4   Limits computationally determined from genome sequence between P{lacW}Gef26k13720 and P{lacW}l(2)k09923k09923&P{lacW}CG9523k07502b
Experimentally Determined Cytological Location
Cytogenetic map Notes References 26D1-26D2     (Lehmann, 2001.3.15)
Experimentally Determined Recombination Data
Location	2L- (Cho, 1998.3.16)
Left of (cM)	P{lacW}l(2)k11101k07502b b (Stein et al., 2002)
Right of (cM)	P{lacW}Gef26k13720 dp (Stein et al., 2002)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\slam for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	EST data suggest additional 5' exon
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) slam-RB FBtr0290281  NM_135176   4093   1173
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein slam-PB   FBpp0288720   130.6   1173   6.09     NP_609020   AAF52374
Additional Polypeptide Data & Comments
Reported size (kDa)	1196 (aa); 135 (kD) (Lecuit et al., 2002)
Comments
External Data
Linkouts
Crossreferences
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA202152   AA202978   AA246298   AA390986   AA392773   AA817250   AA940693   AA940805   AA949111   AA949189   AA949719   AA950412   AA952220   AE014134 AAF52374   AI456528   AI515627   AI515834   AI516776   AI516803   AI518909   AI519881   AI546134   BG638305   BI171505   BI236684   BI356258   BI366068   BI375088   BI587182   BQ103083   BQ103103   BT031138   CK135175   CO177730   CO186035   CO187468   CO263791   CO269531   CO270053   CO281573   CO294913   CO310080   CO310187   CO342428   EC059679   EC069067   EC073431   EC087503   EC194747   EC195641   EC220089   EC224602   EC225064   EC229496   EC244162   EC252303   EC254833   EV585363
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VME5
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 4 -- 5   somatic cell   (Martinho et al., 2004) Comment:NOT germline cell embryonic cycle 13 -- embryonic cycle 14     (Lecuit et al., 2002)
Additional Descriptive Data
	slam is expressed in all somatic cells of the blastoderm embryo, but is excluded from germline cells. (Martinho et al., 2004) Transcript is first detected at embryonic cycle 13 and remains detectable through the slow phase of cellularization. The transcript disappears during the fast phase of cellularization and is no longer detectable at gastrulation. (Lecuit et al., 2002)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
distribution deduced from reporter or direct label Stage Tissue/Position (including subcellular localization) Reference embryonic cycle 14   furrow canal   • plasma membrane & cytoplasm (Lecuit et al., 2002) immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage 4 -- 5   somatic cell   (Martinho et al., 2004) Comment:NOT germline cell mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Dorus et al., 2006, Wasbrough et al., 2010)
Additional Descriptive Data
	Protein is detected in discrete puncta in the cytoplasm and is first detected in the furrow canal region on the membrane of cellularizing embryos. slam protein can also be detected on the adjacent lateral membrane but not apically. (Lecuit et al., 2002) slam is expressed in all somatic cells of the blastoderm embryo, but is excluded from germline cells. (Martinho et al., 2004)
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0043854    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0043854 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of extremely low expression. Peak expression observed within 00-06 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0043854 expression level Developmental Stage   Expression Level embryo 00-02hr    8046 embryo 02-04hr    51502 embryo 04-06hr    1306 embryo 06-08hr    816 embryo 08-10hr    1121 embryo 10-12hr    599 embryo 12-14hr    663 embryo 14-16hr    1071 embryo 16-18hr    759 embryo 18-20hr    416 embryo 20-22hr    1148 embryo 22-24hr    1040 larva L1    16 larva L2    14 larva L3 12hr old    15 larva L3 puffstage 1-2    21 larva L3 puffstage 3-6    24 larva L3 puffstage 7-9    12 white prepupae new    11 white prepupae 12hr    4 white prepupae 24hr    4 pupae 2d postWPP    17 pupae 3d postWPP    5 pupae 4d postWPP    28 adult male 01day    17 adult male 05day    28 adult male 30day    45 adult female 01day    836 adult female 05day    1618 adult female 30day    1755 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (8046) embryo 02-04hr  (51502) embryo 04-06hr  (1306) embryo 06-08hr  (816) embryo 08-10hr  (1121) embryo 10-12hr  (599) embryo 12-14hr  (663) embryo 14-16hr  (1071) embryo 16-18hr  (759) embryo 18-20hr  (416) embryo 20-22hr  (1148) embryo 22-24hr  (1040) larva L1    16 larva L2    14 larva L3 12hr old    15 larva L3 puffstage 1-2    21 larva L3 puffstage 3-6    24 larva L3 puffstage 7-9    12 white prepupae new    11 white prepupae 12hr    4 white prepupae 24hr    4 pupae 2d postWPP    17 pupae 3d postWPP    5 pupae 4d postWPP    28 adult male 01day    17 adult male 05day    28 adult male 30day    45 adult female 01day  (836) adult female 05day  (1618) adult female 30day  (1755) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (8046) embryo 02-04hr  (51502) embryo 04-06hr    1306 embryo 06-08hr    816 embryo 08-10hr    1121 embryo 10-12hr    599 embryo 12-14hr    663 embryo 14-16hr    1071 embryo 16-18hr    759 embryo 18-20hr    416 embryo 20-22hr    1148 embryo 22-24hr    1040 larva L1    16 larva L2    14 larva L3 12hr old    15 larva L3 puffstage 1-2    21 larva L3 puffstage 3-6    24 larva L3 puffstage 7-9    12 white prepupae new    11 white prepupae 12hr    4 white prepupae 24hr    4 pupae 2d postWPP    17 pupae 3d postWPP    5 pupae 4d postWPP    28 adult male 01day    17 adult male 05day    28 adult male 30day    45 adult female 01day    836 adult female 05day  (1618) adult female 30day  (1755) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    8046 embryo 02-04hr  (51502) embryo 04-06hr    1306 embryo 06-08hr    816 embryo 08-10hr    1121 embryo 10-12hr    599 embryo 12-14hr    663 embryo 14-16hr    1071 embryo 16-18hr    759 embryo 18-20hr    416 embryo 20-22hr    1148 embryo 22-24hr    1040 larva L1    16 larva L2    14 larva L3 12hr old    15 larva L3 puffstage 1-2    21 larva L3 puffstage 3-6    24 larva L3 puffstage 7-9    12 white prepupae new    11 white prepupae 12hr    4 white prepupae 24hr    4 pupae 2d postWPP    17 pupae 3d postWPP    5 pupae 4d postWPP    28 adult male 01day    17 adult male 05day    28 adult male 30day    45 adult female 01day    836 adult female 05day    1618 adult female 30day    1755 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    8046 embryo 02-04hr    51502 embryo 04-06hr    1306 embryo 06-08hr    816 embryo 08-10hr    1121 embryo 10-12hr    599 embryo 12-14hr    663 embryo 14-16hr    1071 embryo 16-18hr    759 embryo 18-20hr    416 embryo 20-22hr    1148 embryo 22-24hr    1040 larva L1    16 larva L2    14 larva L3 12hr old    15 larva L3 puffstage 1-2    21 larva L3 puffstage 3-6    24 larva L3 puffstage 7-9    12 white prepupae new    11 white prepupae 12hr    4 white prepupae 24hr    4 pupae 2d postWPP    17 pupae 3d postWPP    5 pupae 4d postWPP    28 adult male 01day    17 adult male 05day    28 adult male 30day    45 adult female 01day    836 adult female 05day    1618 adult female 30day    1755 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0043854 expression level Developmental Stage   Expression Level embryo 00-02hr    8046 embryo 02-04hr    51502 embryo 04-06hr    1306 embryo 06-08hr    816 embryo 08-10hr    1121 embryo 10-12hr    599 embryo 12-14hr    663 embryo 14-16hr    1071 embryo 16-18hr    759 embryo 18-20hr    416 embryo 20-22hr    1148 embryo 22-24hr    1040 larva L1    16 larva L2    14 larva L3 12hr old    15 larva L3 puffstage 1-2    21 larva L3 puffstage 3-6    24 larva L3 puffstage 7-9    12 white prepupae new    11 white prepupae 12hr    4 white prepupae 24hr    4 pupae 2d postWPP    17 pupae 3d postWPP    5 pupae 4d postWPP    28 adult male 01day    17 adult male 05day    28 adult male 30day    45 adult female 01day    836 adult female 05day    1618 adult female 30day    1755 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (8046) embryo 02-04hr  (51502) embryo 04-06hr  (1306) embryo 06-08hr  (816) embryo 08-10hr  (1121) embryo 10-12hr  (599) embryo 12-14hr  (663) embryo 14-16hr  (1071) embryo 16-18hr  (759) embryo 18-20hr  (416) embryo 20-22hr  (1148) embryo 22-24hr  (1040) larva L1    16 larva L2    14 larva L3 12hr old    15 larva L3 puffstage 1-2    21 larva L3 puffstage 3-6    24 larva L3 puffstage 7-9    12 white prepupae new    11 white prepupae 12hr    4 white prepupae 24hr    4 pupae 2d postWPP    17 pupae 3d postWPP    5 pupae 4d postWPP    28 adult male 01day    17 adult male 05day    28 adult male 30day    45 adult female 01day  (836) adult female 05day  (1618) adult female 30day  (1755) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (8046) embryo 02-04hr  (51502) embryo 04-06hr    1306 embryo 06-08hr    816 embryo 08-10hr    1121 embryo 10-12hr    599 embryo 12-14hr    663 embryo 14-16hr    1071 embryo 16-18hr    759 embryo 18-20hr    416 embryo 20-22hr    1148 embryo 22-24hr    1040 larva L1    16 larva L2    14 larva L3 12hr old    15 larva L3 puffstage 1-2    21 larva L3 puffstage 3-6    24 larva L3 puffstage 7-9    12 white prepupae new    11 white prepupae 12hr    4 white prepupae 24hr    4 pupae 2d postWPP    17 pupae 3d postWPP    5 pupae 4d postWPP    28 adult male 01day    17 adult male 05day    28 adult male 30day    45 adult female 01day    836 adult female 05day  1618 adult female 30day  1755 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    8046 embryo 02-04hr  (51502) embryo 04-06hr    1306 embryo 06-08hr    816 embryo 08-10hr    1121 embryo 10-12hr    599 embryo 12-14hr    663 embryo 14-16hr    1071 embryo 16-18hr    759 embryo 18-20hr    416 embryo 20-22hr    1148 embryo 22-24hr    1040 larva L1    16 larva L2    14 larva L3 12hr old    15 larva L3 puffstage 1-2    21 larva L3 puffstage 3-6    24 larva L3 puffstage 7-9    12 white prepupae new    11 white prepupae 12hr    4 white prepupae 24hr    4 pupae 2d postWPP    17 pupae 3d postWPP    5 pupae 4d postWPP    28 adult male 01day    17 adult male 05day    28 adult male 30day    45 adult female 01day    836 adult female 05day    1618 adult female 30day    1755 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    8046 embryo 02-04hr    51502 embryo 04-06hr    1306 embryo 06-08hr    816 embryo 08-10hr    1121 embryo 10-12hr    599 embryo 12-14hr    663 embryo 14-16hr    1071 embryo 16-18hr    759 embryo 18-20hr    416 embryo 20-22hr    1148 embryo 22-24hr    1040 larva L1    16 larva L2    14 larva L3 12hr old    15 larva L3 puffstage 1-2    21 larva L3 puffstage 3-6    24 larva L3 puffstage 7-9    12 white prepupae new    11 white prepupae 12hr    4 white prepupae 24hr    4 pupae 2d postWPP    17 pupae 3d postWPP    5 pupae 4d postWPP    28 adult male 01day    17 adult male 05day    28 adult male 30day    45 adult female 01day    836 adult female 05day    1618 adult female 30day    1755 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0043854    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0043854 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult ovary. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0043854 expression level Tissue   Expression Level Larval Central Nervous System    0.675 Larval Midgut    6.8 Larval Hindgut    3.3 Larval Malpighian Tubules    0.7 Larval Fat Body no informative data Larval Salivary Gland    1.3 Larval Trachea    1.3 Larval Carcass    0.7 Adult Head    3 Adult Eye    3.35 Adult Brain    0.6 Adult Thoracic-Abdominal Ganglion    0.6 Adult Crop    2.6 Adult Midgut    0.7 Adult Hindgut    0.9 Adult Malpighian Tubules    1.3 Adult Fat Body    6.2 Adult Salivary Gland    4 Adult Heart    4.775 Adult VirginFemale Spermatheca    5.4 Adult InseminatedFemale Spermatheca    4.3 Adult Ovary    227.9 Adult Testis    1.7 Adult Male Accessory Gland    4.2 Adult Carcass    6.2 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    0.675 Larval Midgut    6.8 Larval Hindgut    3.3 Larval Malpighian Tubules    0.7 Larval Fat Body no informative data Larval Salivary Gland    1.3 Larval Trachea    1.3 Larval Carcass    0.7 Adult Head    3 Adult Eye    3.35 Adult Brain    0.6 Adult Thoracic-Abdominal Ganglion    0.6 Adult Crop    2.6 Adult Midgut    0.7 Adult Hindgut    0.9 Adult Malpighian Tubules    1.3 Adult Fat Body    6.2 Adult Salivary Gland    4 Adult Heart    4.775 Adult VirginFemale Spermatheca    5.4 Adult InseminatedFemale Spermatheca    4.3 Adult Ovary    227.9 Adult Testis    1.7 Adult Male Accessory Gland    4.2 Adult Carcass    6.2 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    0.675 Larval Midgut    6.8 Larval Hindgut    3.3 Larval Malpighian Tubules    0.7 Larval Fat Body no informative data Larval Salivary Gland    1.3 Larval Trachea    1.3 Larval Carcass    0.7 Adult Head    3 Adult Eye    3.35 Adult Brain    0.6 Adult Thoracic-Abdominal Ganglion    0.6 Adult Crop    2.6 Adult Midgut    0.7 Adult Hindgut    0.9 Adult Malpighian Tubules    1.3 Adult Fat Body    6.2 Adult Salivary Gland    4 Adult Heart    4.775 Adult VirginFemale Spermatheca    5.4 Adult InseminatedFemale Spermatheca    4.3 Adult Ovary    227.9 Adult Testis    1.7 Adult Male Accessory Gland    4.2 Adult Carcass    6.2 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    0.675 Larval Midgut    6.8 Larval Hindgut    3.3 Larval Malpighian Tubules    0.7 Larval Fat Body no informative data Larval Salivary Gland    1.3 Larval Trachea    1.3 Larval Carcass    0.7 Adult Head    3 Adult Eye    3.35 Adult Brain    0.6 Adult Thoracic-Abdominal Ganglion    0.6 Adult Crop    2.6 Adult Midgut    0.7 Adult Hindgut    0.9 Adult Malpighian Tubules    1.3 Adult Fat Body    6.2 Adult Salivary Gland    4 Adult Heart    4.775 Adult VirginFemale Spermatheca    5.4 Adult InseminatedFemale Spermatheca    4.3 Adult Ovary    227.9 Adult Testis    1.7 Adult Male Accessory Gland    4.2 Adult Carcass    6.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0043854 expression level Tissue   Expression Level Larval Central Nervous System    0.675 Larval Midgut    6.8 Larval Hindgut    3.3 Larval Malpighian Tubules    0.7 Larval Fat Body no informative data Larval Salivary Gland    1.3 Larval Trachea    1.3 Larval Carcass    0.7 Adult Head    3 Adult Eye    3.35 Adult Brain    0.6 Adult Thoracic-Abdominal Ganglion    0.6 Adult Crop    2.6 Adult Midgut    0.7 Adult Hindgut    0.9 Adult Malpighian Tubules    1.3 Adult Fat Body    6.2 Adult Salivary Gland    4 Adult Heart    4.775 Adult VirginFemale Spermatheca    5.4 Adult InseminatedFemale Spermatheca    4.3 Adult Ovary    227.9 Adult Testis    1.7 Adult Male Accessory Gland    4.2 Adult Carcass    6.2 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    0.675 Larval Midgut    6.8 Larval Hindgut    3.3 Larval Malpighian Tubules    0.7 Larval Fat Body no informative data Larval Salivary Gland    1.3 Larval Trachea    1.3 Larval Carcass    0.7 Adult Head    3 Adult Eye    3.35 Adult Brain    0.6 Adult Thoracic-Abdominal Ganglion    0.6 Adult Crop    2.6 Adult Midgut    0.7 Adult Hindgut    0.9 Adult Malpighian Tubules    1.3 Adult Fat Body    6.2 Adult Salivary Gland    4 Adult Heart    4.775 Adult VirginFemale Spermatheca    5.4 Adult InseminatedFemale Spermatheca    4.3 Adult Ovary    227.9 Adult Testis    1.7 Adult Male Accessory Gland    4.2 Adult Carcass    6.2 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    0.675 Larval Midgut    6.8 Larval Hindgut    3.3 Larval Malpighian Tubules    0.7 Larval Fat Body no informative data Larval Salivary Gland    1.3 Larval Trachea    1.3 Larval Carcass    0.7 Adult Head    3 Adult Eye    3.35 Adult Brain    0.6 Adult Thoracic-Abdominal Ganglion    0.6 Adult Crop    2.6 Adult Midgut    0.7 Adult Hindgut    0.9 Adult Malpighian Tubules    1.3 Adult Fat Body    6.2 Adult Salivary Gland    4 Adult Heart    4.775 Adult VirginFemale Spermatheca    5.4 Adult InseminatedFemale Spermatheca    4.3 Adult Ovary    227.9 Adult Testis    1.7 Adult Male Accessory Gland    4.2 Adult Carcass    6.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    0.675 Larval Midgut    6.8 Larval Hindgut    3.3 Larval Malpighian Tubules    0.7 Larval Fat Body no informative data Larval Salivary Gland    1.3 Larval Trachea    1.3 Larval Carcass    0.7 Adult Head    3 Adult Eye    3.35 Adult Brain    0.6 Adult Thoracic-Abdominal Ganglion    0.6 Adult Crop    2.6 Adult Midgut    0.7 Adult Hindgut    0.9 Adult Malpighian Tubules    1.3 Adult Fat Body    6.2 Adult Salivary Gland    4 Adult Heart    4.775 Adult VirginFemale Spermatheca    5.4 Adult InseminatedFemale Spermatheca    4.3 Adult Ovary    227.9 Adult Testis    1.7 Adult Male Accessory Gland    4.2 Adult Carcass    6.2 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body no informative data Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    3   NA  Eye    3.35   NA  Brain    0.6   0.675  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    0.6   NA  Crop    2.6   6.8  Midgut    0.7   3.3  Hindgut    0.9   0.7  Malpighian Tubules    1.3   no informative data  Fat Body    6.2   1.3  Salivary Gland    4   NA  Heart    4.775   1.3  Trachea    NA   NA  VirginFemale Spermatheca    5.4   NA  InseminatedFemale Spermatheca    4.3   NA  Ovary    227.9   NA  Testis    1.7   NA  Male Accessory Gland    4.2   0.7  Carcass    6.2 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_01
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0043854 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG9506-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable, with Scer\GAL4pnr-MD237 slamGD14236 Phenotype manifest in Allele cycle 14 embryo (with slamwaldo1) slamwaldo2 cycle 14 embryo (with slamwaldo2) slamwaldo1 embryonic/larval midgut primordium slamunspecified furrow canal | germline clone slam35.16 germline cell slamunspecified slamwaldo1 slamwaldo2 gonadal sheath proper primordium slamunspecified
Classical Alleles ( 5 )
For All Classical Alleles Show
Allele of slam Class Mutagen Stocks Known lesion slamMB06922 1 -- slam35.16 0 -- slamunspecified 0 -- slamwaldo1 ethyl methanesulfonate 0 Yes slamwaldo2 ethyl methanesulfonate 0 Yes
Alleles Carried on Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Allele of slam Class Mutagen Stocks Known lesion slamGD14236 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes slamdsRNA.cLa in vitro construct - RNAi 0 Yes slamScer\UAS.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes slamScer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Not disrupted in	Df(2L)Tigx (Stein et al., 2002)
Disrupted in	Df(2L)Exel6016 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD14236} NA P{UAS-slam.GFP} NA P{UAS-slam.HA} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap binary system Mi{ET1}slamMB06922 No insertion of mobile activating element P{GSV1}GSd406b NA
Gene Ontology: Function, Process & Cellular Component ( 4 unique terms )
Terms Based on Experimental Evidence ( 2 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
cellularization inferred from mutant phenotype (Stein et al., 2002, Lecuit et al., 2002, Wenzl et al., 2010) germ cell migration inferred from mutant phenotype (Stein et al., 2002, Stein et al., 2002)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
cellularization traceable author statement (Gibson and Perrimon, 2003) cellular membrane organization non-traceable author statement (Mazumdar and Mazumdar, 2002) germ cell migration traceable author statement (Mueller, 2002) protein localization non-traceable author statement (Mueller, 2002)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions slam allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 60047 DroID - A comprehensive database of gene and protein interactions. • FBgn0043854 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 33890
Orthologs
Genome-wide drosophilid orthologs	Dana\GF14518 Dere\GG10408 Dgri\GH10746 Dmoj\GI11564 Dper\GL25523 Dpse\GA21841 Dsec\GM18624 Dsim\GD23406 Dvir\GJ12848 Dwil\GK14757 Dyak\GE13835
Curated drosophilid orthologs	Dpse\GA21841
Linkouts	OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • FBgn0231483 FBgn0216762 FBgn0200083 FBgn0194791 FBgn0173530 FBgn0081826 FBgn0163107 FBgn0134324 FBgn0118227 FBgn0102717 FBgn0091545
Stocks & Reagents
Stocks Listed in FlyBase ( 3 )
Bloomington	25533 w1118; Mi{ET1}slamMB06922/SM6a
VDRC	v24049 w1118; P{GD14236}v24049 v24050 w1118; P{GD14236}v24050
Genomic Clones ( 1 )
	BACR05J04 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 42 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD36405
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LD02277 LD03393 LD05160 LD09796 LD11945 LD22808 LD23049 LD27881 LD27991 LD28955 LD29319 LD29709 LD35781 LD36574 LD39716 LD41516 LD42836 LD42874 LD46224 LD47524 RE13294 RE32777 RE42350 RE51512 RE62822
Other clones	Dmel_dig_007022_0683_2972 EK015322 EK029915 EK054142 EK094847 EK097242 EK136441 EK157459 EK207951 EK220416 EK220659 EP14910 LD22450 RH27670 UUGC0480 UUGC0501
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0043854 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 33890
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: slam wdo CG9506 (Stein et al., 2002)
Additional comments
Other Comments
	slam is necessary for polarised growth of membranes during cellularisation. (Lecuit et al., 2002) slam is required for cellularisation and for germ cell migration in the embryo. (Stein et al., 2002) slam controls a key aspect of membrane trafficking in membrane invagination that takes place during embryo cellularization. (Lecuit and Wieschaus, 2001) In slam mutant embryos, the lateral transfer of apical membrane that normally takes place as new membrane is apically inserted is blocked. In addition, the Nrt protein is normally exported from the Golgi but accumulates in an apical compartment instead of being inserted into the recipient apical membrane. (Lecuit and Wieschaus, 2001) Germ cells initiate migration by moving to the interior of the embryo through the posterior midgut epithelium in slam mutant embryos, but they are unable to orient properly on the midgut and subsequently fail to proceed along the correct migratory course. (Cho et al., 1998)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_135176 RefSeq (Proteins) • NP_609020 DDBJ / EMBL / GenBank • AAF52374 BI587182 Entrez Gene - A searchable database of RefSeq genes. • 33890 UniProtKB/TrEMBL • Q9VME5
Other Crossreferences
Linkouts
	BioGRID - A database of protein and genetic interactions • 60047 DroID - A comprehensive database of gene and protein interactions. • FBgn0043854 DRSC - Results from RNAi screens. • FBgn0043854 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0043854 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG9506-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0043854 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 33890 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /cytoskel/slowasmol1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 33890 modMine - Data generated by the modENCODE project. • FBgn0043854 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • FBgn0231483 FBgn0216762 FBgn0200083 FBgn0194791 FBgn0173530 FBgn0081826 FBgn0163107 FBgn0134324 FBgn0118227 FBgn0102717 FBgn0091545
Synonyms & Secondary IDs ( 6 )
Reported As
Symbol Synonym	CG9506 (Arbeitman et al., 2002, Giot et al., 2003, Pilot et al., 2006, Brandt, 2006, Dorus et al., 2006) Slam (Hardin and Walston, 2004, Lecuit et al., 2001, Brandt et al., 2006, Walters et al., 2006, Brandt, 2006) slam (Pilot et al., 2006, Meyer et al., 2006, Takada et al., 2007, Wenzl et al., 2010, Lecuit, 2004, Minidorff et al., 2007, Dorus et al., 2006, Minidorff et al., 2007, Liang et al., 2008, Benoit et al., 2009, Dorus et al., 2006, de Las Heras et al., 2009, Wasbrough et al., 2010) wdo (Lehmann, 2001.3.15, Shen, 1998, Cho, 1998.3.16, Cho et al., 1998, )
Name Synonym	slow as molasses (Lecuit and Wieschaus, 2001, de Las Heras et al., 2009) waldo (Cho et al., 1998)
Secondary FlyBase IDs
	FBgn0024181 FBgn0031806
References ( 60 )
Generate a list of	All references relating to this gene ( 60 )
List References by type	Research paper  ( 19 ) Supplementary material  ( 7 ) Review  ( 13 ) Personal communication to FlyBase  ( 5 ) Abstract  ( 11 ) FlyBase analysis  ( 1 ) Conference report  ( 2 ) Computer file  ( 1 ) Automatic genome annotation  ( 1 )
Recent research papers ( 1 )
	Wenzl et al., 2010, Mech. Dev. 127(7-8): 371--384 Localization of RhoGEF2 during Drosophila cellularization is developmentally controlled by slam. [FBrf0211115] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...