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Gene Dmel\pygo

General Information
Symbol	Dmel\pygo	Species	D. melanogaster
Name	pygopus	Annotation symbol	CG11518
Feature type	protein_coding_gene	FlyBase ID	FBgn0043900
Gene Model Status	Current	Stock availability	9 publicly available
Also Known As	gam
Genomic Location
Chromosome (arm)	3R	Recombination map	3-102
Cytogenetic map	100C6-100C6	Sequence location	3R:27,401,557..27,406,412 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene pygopus is referred to in FlyBase by the symbol Dmel\pygo (CG11518, FBgn0043900). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding; RNA polymerase II transcription coactivator activity. There is experimental evidence for 12 unique biological process terms, many of which group under: biological regulation; single-organism developmental process; post-embryonic organ morphogenesis; cellular process; system development; response to stimulus; cellular component organization or biogenesis; growth; gene expression; cellular nitrogen compound biosynthetic process; regulation of cell adhesion; positive regulation of cellular biosynthetic process; embryonic pattern specification; developmental growth; positive regulation of response to stimulus. 38 alleles are reported. The phenotypes of these alleles are annotated with: organ system; adult segment; organ system subdivision; acellular anatomical structure; thoracic segment; eo support cell; embryonic/larval neuron; imaginal precursor; adult cuticle; external compound sense organ. It has one annotated transcript and one annotated polypeptide. Protein features are: Zinc finger, FYVE/PHD-type; Zinc finger, PHD-finger; Zinc finger, PHD-type; Zinc finger, PHD-type, conserved site; Zinc finger, RING/FYVE/PHD-type. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, adult crop, larval fat body, adult spermathecae, adult testis. Comments on Affy2 ProbeSet: ProbeSet 1640730_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of pygo. Gene sequence location is 3R:27401557..27406412.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 100C6-100C6   Limits computationally determined from genome sequence between P{PZ}l(3)rM731rM731 and P{EP}pygoEP1076
Experimentally Determined Cytological Location
Cytogenetic map Notes References 100C6-100C7     (Kramps, 2001.12.11) 100D-100D     (Parker, 2002.1.24) 100C6-100C7   (determined by in situ hybridisation)   (Abdelilah-Seyfried et al., 2000)
Experimentally Determined Recombination Data
Location	3-102 (Thompson et al., 2002)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\pygo for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.44
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) pygo-RA FBtr0085806  NM_143615   4498   815
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein pygo-PA   FBpp0085167   80.5   815   8.38     NP_651872   AAF57161
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Zinc finger, PHD-type (IPR001965) Zinc finger, FYVE/PHD-type (IPR011011) Zinc finger, PHD-type, conserved site (IPR019786) Zinc finger, PHD-finger (IPR019787) Zinc finger, RING/FYVE/PHD-type (IPR013083)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA536387   AA538694   AA817007   AE014297 AAF57161   AF457206 AAL91369   AI405379   AI517647   AQ025168   AW942476   AX566017   AY058500 AAL13729   AY075095 AAL79357   BF493402   BG638123   BI215318   BI358439   BI362245   CK130297   CO179564   CO192053   CO262286   CO263460   CO264336   CO268131   CO268311   CO268682   CO268863   CO290611   CO301892   CO302014   CO308466   CO341875   CO343542   CZ479557   EC051383   EC052466   EC058903   EC059049   EC059355   EC063215   EC066599   EC070849   EC071113   EC082478   EC208527   EC208806   EC209775   EC211636   EC215045   EC219366   EC241848   EC242772   EC251491   EC260599   EV593383   GH742536   GH742973   GH743076   GH743684   GH743932   GH746197   GH747222   GH761624   GH762377   GH763156   GH766108   GH767202   GH767465   GH767961   GH768053   GH769095   GH769149
UniProtKB/Swiss-Prot	Q9V9W8
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage   organism   (Parker et al., 2002) larval stage   organism   (Parker et al., 2002)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
nucleus inferred from direct assay (Thompson et al., 2002, Hoffmans et al., 2005, Thompson, 2004)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, adult crop, larval fat body, adult spermathecae, adult testis. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    273.9 Larval Midgut    44.1 Larval Hindgut    86.6 Larval Malpighian Tubules    73.3 Larval Fat Body    128.8 Larval Salivary Gland    84.9 Larval Trachea    98.075 Larval Carcass    97.45 Adult Head    82 Adult Eye    64.05 Adult Brain    171.9 Adult Thoracic-Abdominal Ganglion    105.8 Adult Crop    106.5 Adult Midgut    45.4 Adult Hindgut    76.2 Adult Malpighian Tubules    50.7 Adult Fat Body    71.3 Adult Salivary Gland    51.2 Adult Heart    44.725 Adult VirginFemale Spermatheca    115.9 Adult InseminatedFemale Spermatheca    120.6 Adult Ovary    617.8 Adult Testis    476.3 Adult Male Accessory Gland    47.9 Adult Carcass    63.2 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    273.9 Larval Midgut    44.1 Larval Hindgut    86.6 Larval Malpighian Tubules    73.3 Larval Fat Body    128.8 Larval Salivary Gland    84.9 Larval Trachea    98.075 Larval Carcass    97.45 Adult Head    82 Adult Eye    64.05 Adult Brain    171.9 Adult Thoracic-Abdominal Ganglion    105.8 Adult Crop    106.5 Adult Midgut    45.4 Adult Hindgut    76.2 Adult Malpighian Tubules    50.7 Adult Fat Body    71.3 Adult Salivary Gland    51.2 Adult Heart    44.725 Adult VirginFemale Spermatheca    115.9 Adult InseminatedFemale Spermatheca    120.6 Adult Ovary  (617.8) Adult Testis    476.3 Adult Male Accessory Gland    47.9 Adult Carcass    63.2 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    273.9 Larval Midgut    44.1 Larval Hindgut    86.6 Larval Malpighian Tubules    73.3 Larval Fat Body    128.8 Larval Salivary Gland    84.9 Larval Trachea    98.075 Larval Carcass    97.45 Adult Head    82 Adult Eye    64.05 Adult Brain    171.9 Adult Thoracic-Abdominal Ganglion    105.8 Adult Crop    106.5 Adult Midgut    45.4 Adult Hindgut    76.2 Adult Malpighian Tubules    50.7 Adult Fat Body    71.3 Adult Salivary Gland    51.2 Adult Heart    44.725 Adult VirginFemale Spermatheca    115.9 Adult InseminatedFemale Spermatheca    120.6 Adult Ovary    617.8 Adult Testis    476.3 Adult Male Accessory Gland    47.9 Adult Carcass    63.2 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    273.9 Larval Midgut    44.1 Larval Hindgut    86.6 Larval Malpighian Tubules    73.3 Larval Fat Body    128.8 Larval Salivary Gland    84.9 Larval Trachea    98.075 Larval Carcass    97.45 Adult Head    82 Adult Eye    64.05 Adult Brain    171.9 Adult Thoracic-Abdominal Ganglion    105.8 Adult Crop    106.5 Adult Midgut    45.4 Adult Hindgut    76.2 Adult Malpighian Tubules    50.7 Adult Fat Body    71.3 Adult Salivary Gland    51.2 Adult Heart    44.725 Adult VirginFemale Spermatheca    115.9 Adult InseminatedFemale Spermatheca    120.6 Adult Ovary    617.8 Adult Testis    476.3 Adult Male Accessory Gland    47.9 Adult Carcass    63.2 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    273.9 Larval Midgut    44.1 Larval Hindgut    86.6 Larval Malpighian Tubules    73.3 Larval Fat Body    128.8 Larval Salivary Gland    84.9 Larval Trachea    98.075 Larval Carcass    97.45 Adult Head    82 Adult Eye    64.05 Adult Brain    171.9 Adult Thoracic-Abdominal Ganglion    105.8 Adult Crop    106.5 Adult Midgut    45.4 Adult Hindgut    76.2 Adult Malpighian Tubules    50.7 Adult Fat Body    71.3 Adult Salivary Gland    51.2 Adult Heart    44.725 Adult VirginFemale Spermatheca    115.9 Adult InseminatedFemale Spermatheca    120.6 Adult Ovary    617.8 Adult Testis    476.3 Adult Male Accessory Gland    47.9 Adult Carcass    63.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    273.9 Larval Midgut    44.1 Larval Hindgut    86.6 Larval Malpighian Tubules    73.3 Larval Fat Body    128.8 Larval Salivary Gland    84.9 Larval Trachea    98.075 Larval Carcass    97.45 Adult Head    82 Adult Eye    64.05 Adult Brain    171.9 Adult Thoracic-Abdominal Ganglion    105.8 Adult Crop    106.5 Adult Midgut    45.4 Adult Hindgut    76.2 Adult Malpighian Tubules    50.7 Adult Fat Body    71.3 Adult Salivary Gland    51.2 Adult Heart    44.725 Adult VirginFemale Spermatheca    115.9 Adult InseminatedFemale Spermatheca    120.6 Adult Ovary  617.8 Adult Testis    476.3 Adult Male Accessory Gland    47.9 Adult Carcass    63.2 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    273.9 Larval Midgut    44.1 Larval Hindgut    86.6 Larval Malpighian Tubules    73.3 Larval Fat Body    128.8 Larval Salivary Gland    84.9 Larval Trachea    98.075 Larval Carcass    97.45 Adult Head    82 Adult Eye    64.05 Adult Brain    171.9 Adult Thoracic-Abdominal Ganglion    105.8 Adult Crop    106.5 Adult Midgut    45.4 Adult Hindgut    76.2 Adult Malpighian Tubules    50.7 Adult Fat Body    71.3 Adult Salivary Gland    51.2 Adult Heart    44.725 Adult VirginFemale Spermatheca    115.9 Adult InseminatedFemale Spermatheca    120.6 Adult Ovary    617.8 Adult Testis    476.3 Adult Male Accessory Gland    47.9 Adult Carcass    63.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    273.9 Larval Midgut    44.1 Larval Hindgut    86.6 Larval Malpighian Tubules    73.3 Larval Fat Body    128.8 Larval Salivary Gland    84.9 Larval Trachea    98.075 Larval Carcass    97.45 Adult Head    82 Adult Eye    64.05 Adult Brain    171.9 Adult Thoracic-Abdominal Ganglion    105.8 Adult Crop    106.5 Adult Midgut    45.4 Adult Hindgut    76.2 Adult Malpighian Tubules    50.7 Adult Fat Body    71.3 Adult Salivary Gland    51.2 Adult Heart    44.725 Adult VirginFemale Spermatheca    115.9 Adult InseminatedFemale Spermatheca    120.6 Adult Ovary    617.8 Adult Testis    476.3 Adult Male Accessory Gland    47.9 Adult Carcass    63.2 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    82   NA  Eye    64.05   NA  Brain    171.9   273.9  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    105.8   NA  Crop    106.5   44.1  Midgut    45.4   86.6  Hindgut    76.2   73.3  Malpighian Tubules    50.7   128.8  Fat Body    71.3   84.9  Salivary Gland    51.2   NA  Heart    44.725   98.075  Trachea    NA   NA  VirginFemale Spermatheca    115.9   NA  InseminatedFemale Spermatheca    120.6   NA  Ovary    617.8   NA  Testis    476.3   NA  Male Accessory Gland    47.9   97.45  Carcass    63.2 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    33 central nervous system, larvae L3    27 central nervous system, pupae P8    15 head, virgin 1-day female    8 head, virgin 4-day female    9 head, virgin 20-day female    6 head, mated 1-day female    11 head, mated 4-day female    6 head, mated 20-day female    7 head, mated 1-day male    10 head, mated 4-day male    11 head, mated 20-day male    9 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    5 digestive system, 4-day adult    7 digestive system, 20-day adult    6 fat body, larvae L3 wandering    4 fat body, white prepupae    13 fat body, pupae P8    14 carcass, larvae L3 wandering    15 carcass, 1-day adult    6 carcass, 4-day adult    9 carcass, 20-day adult    9 ovary, virgin 4-day female    81 ovary, mated 4-day female    91 testis, mated 4-day male    40 accessory gland, mated 4-day male    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (33) central nervous system, larvae L3  (27) central nervous system, pupae P8    15 head, virgin 1-day female    8 head, virgin 4-day female    9 head, virgin 20-day female    6 head, mated 1-day female    11 head, mated 4-day female    6 head, mated 20-day female    7 head, mated 1-day male    10 head, mated 4-day male    11 head, mated 20-day male    9 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    5 digestive system, 4-day adult    7 digestive system, 20-day adult    6 fat body, larvae L3 wandering    4 fat body, white prepupae    13 fat body, pupae P8    14 carcass, larvae L3 wandering    15 carcass, 1-day adult    6 carcass, 4-day adult    9 carcass, 20-day adult    9 ovary, virgin 4-day female  (81) ovary, mated 4-day female  (91) testis, mated 4-day male  (40) accessory gland, mated 4-day male    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    33 central nervous system, larvae L3    27 central nervous system, pupae P8    15 head, virgin 1-day female    8 head, virgin 4-day female    9 head, virgin 20-day female    6 head, mated 1-day female    11 head, mated 4-day female    6 head, mated 20-day female    7 head, mated 1-day male    10 head, mated 4-day male    11 head, mated 20-day male    9 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    5 digestive system, 4-day adult    7 digestive system, 20-day adult    6 fat body, larvae L3 wandering    4 fat body, white prepupae    13 fat body, pupae P8    14 carcass, larvae L3 wandering    15 carcass, 1-day adult    6 carcass, 4-day adult    9 carcass, 20-day adult    9 ovary, virgin 4-day female    81 ovary, mated 4-day female    91 testis, mated 4-day male    40 accessory gland, mated 4-day male    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    33 central nervous system, larvae L3    27 central nervous system, pupae P8    15 head, virgin 1-day female    8 head, virgin 4-day female    9 head, virgin 20-day female    6 head, mated 1-day female    11 head, mated 4-day female    6 head, mated 20-day female    7 head, mated 1-day male    10 head, mated 4-day male    11 head, mated 20-day male    9 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    5 digestive system, 4-day adult    7 digestive system, 20-day adult    6 fat body, larvae L3 wandering    4 fat body, white prepupae    13 fat body, pupae P8    14 carcass, larvae L3 wandering    15 carcass, 1-day adult    6 carcass, 4-day adult    9 carcass, 20-day adult    9 ovary, virgin 4-day female    81 ovary, mated 4-day female    91 testis, mated 4-day male    40 accessory gland, mated 4-day male    8 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    33 central nervous system, larvae L3    27 central nervous system, pupae P8    15 head, virgin 1-day female    8 head, virgin 4-day female    9 head, virgin 20-day female    6 head, mated 1-day female    11 head, mated 4-day female    6 head, mated 20-day female    7 head, mated 1-day male    10 head, mated 4-day male    11 head, mated 20-day male    9 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    5 digestive system, 4-day adult    7 digestive system, 20-day adult    6 fat body, larvae L3 wandering    4 fat body, white prepupae    13 fat body, pupae P8    14 carcass, larvae L3 wandering    15 carcass, 1-day adult    6 carcass, 4-day adult    9 carcass, 20-day adult    9 ovary, virgin 4-day female    81 ovary, mated 4-day female    91 testis, mated 4-day male    40 accessory gland, mated 4-day male    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (33) central nervous system, larvae L3  27 central nervous system, pupae P8    15 head, virgin 1-day female    8 head, virgin 4-day female    9 head, virgin 20-day female    6 head, mated 1-day female    11 head, mated 4-day female    6 head, mated 20-day female    7 head, mated 1-day male    10 head, mated 4-day male    11 head, mated 20-day male    9 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    5 digestive system, 4-day adult    7 digestive system, 20-day adult    6 fat body, larvae L3 wandering    4 fat body, white prepupae    13 fat body, pupae P8    14 carcass, larvae L3 wandering    15 carcass, 1-day adult    6 carcass, 4-day adult    9 carcass, 20-day adult    9 ovary, virgin 4-day female  (81) ovary, mated 4-day female  (91) testis, mated 4-day male  (40) accessory gland, mated 4-day male    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    33 central nervous system, larvae L3    27 central nervous system, pupae P8    15 head, virgin 1-day female    8 head, virgin 4-day female    9 head, virgin 20-day female    6 head, mated 1-day female    11 head, mated 4-day female    6 head, mated 20-day female    7 head, mated 1-day male    10 head, mated 4-day male    11 head, mated 20-day male    9 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    5 digestive system, 4-day adult    7 digestive system, 20-day adult    6 fat body, larvae L3 wandering    4 fat body, white prepupae    13 fat body, pupae P8    14 carcass, larvae L3 wandering    15 carcass, 1-day adult    6 carcass, 4-day adult    9 carcass, 20-day adult    9 ovary, virgin 4-day female    81 ovary, mated 4-day female    91 testis, mated 4-day male    40 accessory gland, mated 4-day male    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    33 central nervous system, larvae L3    27 central nervous system, pupae P8    15 head, virgin 1-day female    8 head, virgin 4-day female    9 head, virgin 20-day female    6 head, mated 1-day female    11 head, mated 4-day female    6 head, mated 20-day female    7 head, mated 1-day male    10 head, mated 4-day male    11 head, mated 20-day male    9 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    5 digestive system, 4-day adult    7 digestive system, 20-day adult    6 fat body, larvae L3 wandering    4 fat body, white prepupae    13 fat body, pupae P8    14 carcass, larvae L3 wandering    15 carcass, 1-day adult    6 carcass, 4-day adult    9 carcass, 20-day adult    9 ovary, virgin 4-day female    81 ovary, mated 4-day female    91 testis, mated 4-day male    40 accessory gland, mated 4-day male    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    61 embryo 02-04hr    68 embryo 04-06hr    30 embryo 06-08hr    29 embryo 08-10hr    28 embryo 10-12hr    21 embryo 12-14hr    17 embryo 14-16hr    20 embryo 16-18hr    16 embryo 18-20hr    10 embryo 20-22hr    27 embryo 22-24hr    20 larva L1    5 larva L2    4 larva L3 12hr old    3 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    9 white prepupae new    9 white prepupae 12hr    11 white prepupae 24hr    14 pupae 2d postWPP    10 pupae 3d postWPP    6 pupae 4d postWPP    4 adult male 01day    6 adult male 05day    9 adult male 30day    7 adult female 01day    14 adult female 05day    30 adult female 30day    32 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (61) embryo 02-04hr  (68) embryo 04-06hr  (30) embryo 06-08hr  (29) embryo 08-10hr  (28) embryo 10-12hr    21 embryo 12-14hr    17 embryo 14-16hr    20 embryo 16-18hr    16 embryo 18-20hr    10 embryo 20-22hr  (27) embryo 22-24hr    20 larva L1    5 larva L2    4 larva L3 12hr old    3 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    9 white prepupae new    9 white prepupae 12hr    11 white prepupae 24hr    14 pupae 2d postWPP    10 pupae 3d postWPP    6 pupae 4d postWPP    4 adult male 01day    6 adult male 05day    9 adult male 30day    7 adult female 01day    14 adult female 05day  (30) adult female 30day  (32) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    61 embryo 02-04hr    68 embryo 04-06hr    30 embryo 06-08hr    29 embryo 08-10hr    28 embryo 10-12hr    21 embryo 12-14hr    17 embryo 14-16hr    20 embryo 16-18hr    16 embryo 18-20hr    10 embryo 20-22hr    27 embryo 22-24hr    20 larva L1    5 larva L2    4 larva L3 12hr old    3 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    9 white prepupae new    9 white prepupae 12hr    11 white prepupae 24hr    14 pupae 2d postWPP    10 pupae 3d postWPP    6 pupae 4d postWPP    4 adult male 01day    6 adult male 05day    9 adult male 30day    7 adult female 01day    14 adult female 05day    30 adult female 30day    32 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    61 embryo 02-04hr    68 embryo 04-06hr    30 embryo 06-08hr    29 embryo 08-10hr    28 embryo 10-12hr    21 embryo 12-14hr    17 embryo 14-16hr    20 embryo 16-18hr    16 embryo 18-20hr    10 embryo 20-22hr    27 embryo 22-24hr    20 larva L1    5 larva L2    4 larva L3 12hr old    3 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    9 white prepupae new    9 white prepupae 12hr    11 white prepupae 24hr    14 pupae 2d postWPP    10 pupae 3d postWPP    6 pupae 4d postWPP    4 adult male 01day    6 adult male 05day    9 adult male 30day    7 adult female 01day    14 adult female 05day    30 adult female 30day    32 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    61 embryo 02-04hr    68 embryo 04-06hr    30 embryo 06-08hr    29 embryo 08-10hr    28 embryo 10-12hr    21 embryo 12-14hr    17 embryo 14-16hr    20 embryo 16-18hr    16 embryo 18-20hr    10 embryo 20-22hr    27 embryo 22-24hr    20 larva L1    5 larva L2    4 larva L3 12hr old    3 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    9 white prepupae new    9 white prepupae 12hr    11 white prepupae 24hr    14 pupae 2d postWPP    10 pupae 3d postWPP    6 pupae 4d postWPP    4 adult male 01day    6 adult male 05day    9 adult male 30day    7 adult female 01day    14 adult female 05day    30 adult female 30day    32 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (61) embryo 02-04hr  (68) embryo 04-06hr  30 embryo 06-08hr  29 embryo 08-10hr  28 embryo 10-12hr    21 embryo 12-14hr    17 embryo 14-16hr    20 embryo 16-18hr    16 embryo 18-20hr    10 embryo 20-22hr  27 embryo 22-24hr    20 larva L1    5 larva L2    4 larva L3 12hr old    3 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    9 white prepupae new    9 white prepupae 12hr    11 white prepupae 24hr    14 pupae 2d postWPP    10 pupae 3d postWPP    6 pupae 4d postWPP    4 adult male 01day    6 adult male 05day    9 adult male 30day    7 adult female 01day    14 adult female 05day  30 adult female 30day  32 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    61 embryo 02-04hr    68 embryo 04-06hr    30 embryo 06-08hr    29 embryo 08-10hr    28 embryo 10-12hr    21 embryo 12-14hr    17 embryo 14-16hr    20 embryo 16-18hr    16 embryo 18-20hr    10 embryo 20-22hr    27 embryo 22-24hr    20 larva L1    5 larva L2    4 larva L3 12hr old    3 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    9 white prepupae new    9 white prepupae 12hr    11 white prepupae 24hr    14 pupae 2d postWPP    10 pupae 3d postWPP    6 pupae 4d postWPP    4 adult male 01day    6 adult male 05day    9 adult male 30day    7 adult female 01day    14 adult female 05day    30 adult female 30day    32 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    61 embryo 02-04hr    68 embryo 04-06hr    30 embryo 06-08hr    29 embryo 08-10hr    28 embryo 10-12hr    21 embryo 12-14hr    17 embryo 14-16hr    20 embryo 16-18hr    16 embryo 18-20hr    10 embryo 20-22hr    27 embryo 22-24hr    20 larva L1    5 larva L2    4 larva L3 12hr old    3 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    9 white prepupae new    9 white prepupae 12hr    11 white prepupae 24hr    14 pupae 2d postWPP    10 pupae 3d postWPP    6 pupae 4d postWPP    4 adult male 01day    6 adult male 05day    9 adult male 30day    7 adult female 01day    14 adult female 05day    30 adult female 30day    32 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    25 Schneider line 2 Sg4    19 embryonic 1182-4H    13 embryonic GM2    9 embryonic Kc167    34 embryonic S1    18 embryonic S3    16 leg disc CME L1    15 wing disc CME-W2    6 wing disc ML-DmD8    16 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    21 wing disc ML-DmD21    15 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    20 eye-antennal disc ML-DmD11    14 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    18 CNS ML-DmBG1-c1    15 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    16 ovary fGS/OSS    20 ovary OSC    20 ovary OSS    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    25 Schneider line 2 Sg4    19 embryonic 1182-4H    13 embryonic GM2    9 embryonic Kc167  (34) embryonic S1    18 embryonic S3    16 leg disc CME L1    15 wing disc CME-W2    6 wing disc ML-DmD8    16 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    21 wing disc ML-DmD21    15 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    20 eye-antennal disc ML-DmD11    14 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    18 CNS ML-DmBG1-c1    15 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    16 ovary fGS/OSS    20 ovary OSC    20 ovary OSS    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    25 Schneider line 2 Sg4    19 embryonic 1182-4H    13 embryonic GM2    9 embryonic Kc167    34 embryonic S1    18 embryonic S3    16 leg disc CME L1    15 wing disc CME-W2    6 wing disc ML-DmD8    16 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    21 wing disc ML-DmD21    15 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    20 eye-antennal disc ML-DmD11    14 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    18 CNS ML-DmBG1-c1    15 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    16 ovary fGS/OSS    20 ovary OSC    20 ovary OSS    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    25 Schneider line 2 Sg4    19 embryonic 1182-4H    13 embryonic GM2    9 embryonic Kc167    34 embryonic S1    18 embryonic S3    16 leg disc CME L1    15 wing disc CME-W2    6 wing disc ML-DmD8    16 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    21 wing disc ML-DmD21    15 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    20 eye-antennal disc ML-DmD11    14 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    18 CNS ML-DmBG1-c1    15 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    16 ovary fGS/OSS    20 ovary OSC    20 ovary OSS    11 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    25 Schneider line 2 Sg4    19 embryonic 1182-4H    13 embryonic GM2    9 embryonic Kc167    34 embryonic S1    18 embryonic S3    16 leg disc CME L1    15 wing disc CME-W2    6 wing disc ML-DmD8    16 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    21 wing disc ML-DmD21    15 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    20 eye-antennal disc ML-DmD11    14 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    18 CNS ML-DmBG1-c1    15 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    16 ovary fGS/OSS    20 ovary OSC    20 ovary OSS    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    25 Schneider line 2 Sg4    19 embryonic 1182-4H    13 embryonic GM2    9 embryonic Kc167  (34) embryonic S1    18 embryonic S3    16 leg disc CME L1    15 wing disc CME-W2    6 wing disc ML-DmD8    16 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    21 wing disc ML-DmD21    15 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    20 eye-antennal disc ML-DmD11    14 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    18 CNS ML-DmBG1-c1    15 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    16 ovary fGS/OSS    20 ovary OSC    20 ovary OSS    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    25 Schneider line 2 Sg4    19 embryonic 1182-4H    13 embryonic GM2    9 embryonic Kc167    34 embryonic S1    18 embryonic S3    16 leg disc CME L1    15 wing disc CME-W2    6 wing disc ML-DmD8    16 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    21 wing disc ML-DmD21    15 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    20 eye-antennal disc ML-DmD11    14 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    18 CNS ML-DmBG1-c1    15 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    16 ovary fGS/OSS    20 ovary OSC    20 ovary OSS    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    25 Schneider line 2 Sg4    19 embryonic 1182-4H    13 embryonic GM2    9 embryonic Kc167    34 embryonic S1    18 embryonic S3    16 leg disc CME L1    15 wing disc CME-W2    6 wing disc ML-DmD8    16 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    21 wing disc ML-DmD21    15 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    20 eye-antennal disc ML-DmD11    14 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    18 CNS ML-DmBG1-c1    15 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    16 ovary fGS/OSS    20 ovary OSC    20 ovary OSS    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    17 cold shock, 4-day adult    14 heat shock, 4-day adult    19 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    26 Cadmium 100 mM 48 hrs, 4-day adult    68 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    68 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    27 Ethanol 2.5% 3 hrs, larvae L3    40 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    63 Caffeine 25 mg/ml 48 hrs, 4-day adult    26 Paraquat 5 mM 48 hrs, 4-day adult    45 Paraquat 10 mM 48 hrs, 4-day adult    30 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    17 cold shock, 4-day adult    14 heat shock, 4-day adult    19 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult  26 Cadmium 100 mM 48 hrs, 4-day adult  (68) Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult  (68) Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult  (27) Ethanol 2.5% 3 hrs, larvae L3  (40) Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (63) Caffeine 25 mg/ml 48 hrs, 4-day adult  26 Paraquat 5 mM 48 hrs, 4-day adult  (45) Paraquat 10 mM 48 hrs, 4-day adult  (30) Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    17 cold shock, 4-day adult    14 heat shock, 4-day adult    19 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    26 Cadmium 100 mM 48 hrs, 4-day adult    68 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    68 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    27 Ethanol 2.5% 3 hrs, larvae L3    40 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    63 Caffeine 25 mg/ml 48 hrs, 4-day adult    26 Paraquat 5 mM 48 hrs, 4-day adult    45 Paraquat 10 mM 48 hrs, 4-day adult    30 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    17 cold shock, 4-day adult    14 heat shock, 4-day adult    19 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    26 Cadmium 100 mM 48 hrs, 4-day adult    68 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    68 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    27 Ethanol 2.5% 3 hrs, larvae L3    40 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    63 Caffeine 25 mg/ml 48 hrs, 4-day adult    26 Paraquat 5 mM 48 hrs, 4-day adult    45 Paraquat 10 mM 48 hrs, 4-day adult    30 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    17 cold shock, 4-day adult    14 heat shock, 4-day adult    19 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    26 Cadmium 100 mM 48 hrs, 4-day adult    68 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    68 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    27 Ethanol 2.5% 3 hrs, larvae L3    40 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    63 Caffeine 25 mg/ml 48 hrs, 4-day adult    26 Paraquat 5 mM 48 hrs, 4-day adult    45 Paraquat 10 mM 48 hrs, 4-day adult    30 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    17 cold shock, 4-day adult    14 heat shock, 4-day adult    19 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult  26 Cadmium 100 mM 48 hrs, 4-day adult  (68) Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult  (68) Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult  27 Ethanol 2.5% 3 hrs, larvae L3  (40) Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (63) Caffeine 25 mg/ml 48 hrs, 4-day adult  26 Paraquat 5 mM 48 hrs, 4-day adult  (45) Paraquat 10 mM 48 hrs, 4-day adult  30 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    17 cold shock, 4-day adult    14 heat shock, 4-day adult    19 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    26 Cadmium 100 mM 48 hrs, 4-day adult    68 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    68 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    27 Ethanol 2.5% 3 hrs, larvae L3    40 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    63 Caffeine 25 mg/ml 48 hrs, 4-day adult    26 Paraquat 5 mM 48 hrs, 4-day adult    45 Paraquat 10 mM 48 hrs, 4-day adult    30 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    17 cold shock, 4-day adult    14 heat shock, 4-day adult    19 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    26 Cadmium 100 mM 48 hrs, 4-day adult    68 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    68 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    27 Ethanol 2.5% 3 hrs, larvae L3    40 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    63 Caffeine 25 mg/ml 48 hrs, 4-day adult    26 Paraquat 5 mM 48 hrs, 4-day adult    45 Paraquat 10 mM 48 hrs, 4-day adult    30 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_17 mE1_20_mRNA_expression_cluster_04
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0043900 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG11518-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal pygoGD1516 lethal (with pygoS28) pygoS123 lethal (with pygoS123) pygoS28 lethal | embryonic stage | germline clone | maternal effect pygo10 lethal | embryonic stage | maternal effect | recessive pygoF15 pygoF66 pygoF107 lethal | germline clone | recessive | segment polarity expression pattern pygo130 lethal | larval stage | recessive pygounspecified lethal | pupal stage, with Scer\GAL4[-]: pygoEP1076 lethal | pupal stage (with pygo9) pygo10 lethal | pupal stage (with pygo10) pygo9 lethal | pupal stage | recessive pygo10 pygo130 pygounspecified partially lethal - majority die | pupal stage, with Scer\GAL4pnr-MD237 pygoGD1516 Other Phenotypes Allele cell growth defective | somatic clone pygoS28 cell shape defective | pupal stage | somatic clone pygoS123 cell shape defective | somatic clone | third instar larval stage pygoS123 decreased cell death | somatic clone pygo10 increased cell death | somatic clone pygoS28 neuroanatomy defective | larval stage pygoGD1516 visible, with Scer\GAL4en-e16E pygoEP1076 visible, with Scer\GAL4ey.PH pygoEP1076 visible, with Scer\GAL4pnr-MD237 pygoGD1516 visible, with Scer\GAL4ptc-559.1 pygoScer\UAS.cPa visible, with Scer\GAL4sca-537.4 pygoEP1076 visible | recessive | somatic clone pygoF66 visible | somatic clone pygoS28 wild-type pygoαTub84B.PK Phenotype manifest in Allele adult cuticle, with Scer\GAL4[-]: pygoEP1076 cone cell | pupal stage | somatic clone pygoS123 cone cell | somatic clone | third instar larval stage pygoS123 cuticle pygo10 denticle | ectopic pygoS28 denticle | ectopic | germline clone | rescuable maternal effect pygoS123 denticle belt pygo10 denticle belt | maternal effect pygoF15 pygoF66 denticle belt | rescuable maternal effect pygoF107 dorsocentral bristle, with Scer\GAL4sca-537.4 pygoEP1076 embryo | maternal effect | pair rule expression pattern pygoF15 pygoF66 embryo | maternal effect | segment polarity expression pattern pygoF15 pygoF66 embryo | rescuable maternal effect | segment polarity expression pattern pygoF107 embryonic/first instar larval cuticle pygoS28 embryonic/first instar larval cuticle | germline clone pygoS123 pygoS28 embryonic/first instar larval cuticle | germline clone | rescuable maternal effect pygoS123 embryonic/first instar larval cuticle | maternal effect pygoF15 pygoF66 pygoS123 pygoS28 embryonic/first instar larval cuticle | rescuable maternal effect pygoF107 embryonic/larval neuroblast | larval stage pygoGD1516 embryonic epidermis pygo10 eye, with Scer\GAL4ey.PH pygoEP1076 eye photoreceptor cell | P-stage | ectopic | somatic clone pygo10 ganglion mother cell | larval stage pygoGD1516 imaginal disc pygo10 leg, with Scer\GAL4[-]: pygoEP1076 leg | distal | somatic clone pygoS28 mesothoracic tergum, with Scer\GAL4pnr-MD237 pygoGD1516 midgut constriction 2 | germline clone pygoS123 pygoS28 ommatidium | P-stage | ectopic | somatic clone pygo10 photoreceptor cell | ectopic | somatic clone pygo10 RP2 motor neuron pygounspecified scutellar bristle, with Scer\GAL4sca-537.4 pygoEP1076 sex comb, with Scer\GAL4[-]: pygoEP1076 trichogen cell, with Scer\GAL4pnr-MD237 pygoGD1516 wing, with Scer\GAL430A pygoScer\UAS.cPa wing, with Scer\GAL4en-e16E pygoEP1076 wing | somatic clone pygo10 pygoF66 pygoS28 wing disc | somatic clone pygoS28 wing margin, with Scer\GAL4ptc-559.1 pygoScer\UAS.cPa wing margin | somatic clone pygo10 pygoS28 wing margin bristle | cell non-autonomous | ectopic | somatic clone pygoF66
Classical Alleles ( 15 )
For All Classical Alleles Show
Allele of pygo Class Mutagen Stocks Known lesion pygo10 Delta2-3 1 Yes pygoCB-6097-3 1 -- pygoS123 loss of function allele ethyl methanesulfonate 1 Yes pygoS28 loss of function allele, hypomorphic allele - genetic evidence ethyl methanesulfonate 1 Yes pygo11-3 0 Yes pygo130 ethyl methanesulfonate 0 Yes pygo577 Delta2-3 0 Yes pygo677 Delta2-3 0 Yes pygo9 Delta2-3 0 Yes pygoe01745 piggyBac transposase 0 -- pygoEP1076 P-element activity 0 Yes pygoF107 ethyl methanesulfonate 0 Yes pygoF15 loss of function allele ethyl methanesulfonate 0 Yes pygoF66 ethyl methanesulfonate 0 Yes pygounspecified 0 --
Alleles Carried on Transgenic Constructs ( 23 )
For All Alleles Carried on Transgenic Constructs Show
Allele of pygo Class Mutagen Stocks Known lesion pygoGD1516 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes pygoGL00647 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes pygoKK108114 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes pygoScer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 1 Yes pygo+tHa in vitro construct - genomic fragment 0 Yes pygoA763E.T:Ivir\HA1 in vitro construct - amino acid replacement 0 Yes pygoAct5C.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes pygodsRNA.cMa in vitro construct - RNAi 0 -- pygodsRNA.cTa in vitro construct - RNAi 0 Yes pygoF78A in vitro construct - site directed mutagenesis 0 Yes pygoF99A.tHa in vitro construct - amino acid replacement 0 Yes pygoL789A.T:Ivir\HA1 in vitro construct - amino acid replacement 0 Yes pygomLoop1.Scer\UAS.T:Ivir\HA1 in vitro construct - amino acid replacement 0 Yes pygomLoop2.Scer\UAS.T:Ivir\HA1 in vitro construct - amino acid replacement 0 Yes pygoNIG.11518R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes pygoNnpf.Scer\UAS.T:Ivir\HA1 in vitro construct - amino acid replacement 0 Yes pygoScer\UAS.cPa in vitro construct - regulatory fusion 0 Yes pygoT:Ivir\HA1 in vitro construct - coding region fusion 0 Yes pygoV757E.T:Ivir\HA1 in vitro construct - amino acid replacement 0 Yes pygoY748A.A763E.T:Ivir\HA1 in vitro construct - amino acid replacement 0 Yes pygoY748A.F773E.T:Ivir\HA1 in vitro construct - amino acid replacement 0 Yes pygoY748A.T:Ivir\HA1 in vitro construct - amino acid replacement 0 Yes pygoαTub84B.PK in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Not disrupted in	Df(3R)awd-KRB (Cook, 2006.1.31) Df(3R)tll-e (Cook, 2006.1.31) Df(3R)tll-g (Cook, 2006.1.31)
Partially disrupted in	Df(3R)pygo11-3 (Grieder et al., 2008) Df(3R)γCop577 (Grieder et al., 2008) Df(3R)γCop677 (Grieder et al., 2008)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD1516} NA P{KK108114} NA P{NIG.11518R} NA P{TRiP.GL00647} NA P{UAS-pygo.HA} NA P{UAS-pygo.mLoop1} NA P{UAS-pygo.mLoop2} NA P{UAS-pygo.Nnpf} NA P{UAS-pygo.P} NA characterization construct P{pygo+tHa} NA P{pygoA763E.T:Ivir\HA1} NA P{pygoF78A} NA P{pygoF99A.tHa} NA P{pygoL789A.T:Ivir\HA1} NA P{pygoT:Ivir\HA1} NA P{pygoV757E.T:Ivir\HA1} NA P{pygoY748A.A763E.T:Ivir\HA1} NA P{pygoY748A.F773E.T:Ivir\HA1} NA P{pygoY748A.T:Ivir\HA1} NA P{pygo-pygo::lgs.wt} NA P{pygo-pygo::lgs.ΔHD1.Y748A.L789A} NA P{pygo-pygo::lgs.ΔHD1.ΔHD2} NA P{pygo-pygo::lgs.ΔHD1} NA P{pygo-pygo::lgs.ΔPHD-HD1} NA P{UAS-pygoΔPHD-ΔNpan} NA P{αTub84B-lgs::pygo.ΔPHD.HD2} NA P{αTub84B-pygo.K} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}pygoEP1076 NA P{EP}rodEP3408 NA miscellaneous insertions P{5'lArB}γCop677 NA P{RS3}pygoCB-6097-3 NA P{SUPor-P}CG3558KG00037 NA PBac{RB}pygoe01745 NA
Gene Ontology: Function, Process & Cellular Component ( 16 unique terms )
Terms Based on Experimental Evidence ( 15 terms )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with lgs (assigned by UniProtKB) (Kramps et al., 2002) contributes_to RNA polymerase II transcription coactivator activity inferred from physical interaction with arm (Thompson, 2004) inferred from physical interaction with lgs (Thompson, 2004)
Biological Process
CV term Evidence References
delamination inferred from mutant phenotype (Kiger et al., 2007) dendrite morphogenesis inferred from mutant phenotype (Parrish et al., 2006) embryonic pattern specification inferred from mutant phenotype (Thompson et al., 2002) eye-antennal disc development inferred from mutant phenotype (Lin et al., 2004) imaginal disc-derived wing expansion inferred from mutant phenotype (Kiger et al., 2007) neurogenesis inferred from mutant phenotype (Neumüller et al., 2011) positive regulation of transcription from RNA polymerase II promoter inferred from direct assay (Thompson, 2004) positive regulation of Wnt receptor signaling pathway inferred from genetic interaction with lgs AND inferred from genetic interaction with wg (assigned by UniProtKB) (Kramps et al., 2002) inferred from mutant phenotype (Carrera et al., 2008) transcription, DNA-dependent inferred from mutant phenotype (Thompson et al., 2002) wing disc morphogenesis inferred from mutant phenotype (Kiger et al., 2007) Wnt receptor signaling pathway inferred from genetic interaction with arm (Thompson et al., 2002) inferred from genetic interaction with Axn AND inferred from genetic interaction with arm AND inferred from genetic interaction with wg AND inferred from mutant phenotype (Parker et al., 2002) inferred from mutant phenotype (Belenkaya et al., 2002) inferred from mutant phenotype AND inferred from physical interaction with lgs (assigned by UniProtKB) (Kramps et al., 2002) Wnt receptor signaling pathway, calcium modulating pathway inferred from mutant phenotype (Parker et al., 2002)
Cellular Component
CV term Evidence References
nucleus inferred from direct assay (Thompson et al., 2002, Hoffmans et al., 2005, Thompson, 2004)
Terms Based on Predictions or Assertions ( 3 terms )
Molecular Function
CV term Evidence References
zinc ion binding inferred from electronic annotation with InterPro:IPR001965 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
Wnt receptor signaling pathway non-traceable author statement (Parker, 2002.1.24, Cadigan, 2002.1.24, Kramps, 2001.12.11)
Cellular Component
CV term Evidence References
nucleus non-traceable author statement (Parker, 2002.1.24, Kramps, 2001.12.11) non-traceable author statement (assigned by UniProtKB) (Kramps et al., 2002)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
pygo - ebd1 pull down, anti tag western blot, anti tag coimmunoprecipitation (Benchabane et al., 2011)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions pygo allele Gene References pygo10 Axn (Parker et al., 2002) pygo130 wg (Kramps et al., 2002) pygoEP1076 arm (Parker et al., 2002, Thompson et al., 2002) CycE (Tseng and Hariharan, 2002) wg (Parker et al., 2002) pygoS28 arm (Thompson et al., 2002, Townsley et al., 2004) pan::pygo (Thompson, 2004) pygoS123 arm (Thompson et al., 2002, Townsley et al., 2004) pygoScer\UAS.cPa arm (Parker et al., 2002) wg (Parker et al., 2002) pygounspecified Axn (Parker et al., 2002) unspecified arm (Cadigan, 2002.1.24) Axn (Cadigan, 2002.1.24) dsh (Cadigan, 2002.1.24) wg (Cadigan et al., 2001)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 68561 DroID - A comprehensive database of gene and protein interactions. • FBgn0043900 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43718 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q9V9W8
Orthologs
OrthoDB Orthologs (63) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6FTWKB Dipteran inclusive ortholog search EOG6D26Z5 Insect inclusive ortholog search EOG62V7B2 Arthropod inclusive ortholog search EOG6QBZM4 Metazoa inclusive ortholog search EOG6W45DM
Orthologs in Drosophila Species (EOG6FTWKB)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\pygo     Drosophila melanogaster fruit fly  Dmel\futsch     Drosophila melanogaster fruit fly  Dmel\A2bp1     Drosophila melanogaster fruit fly  Dmel\CG13068     Drosophila erecta   Dere\GG17842   Y Drosophila erecta   Dere\GG23534   Y Drosophila erecta   Dere\GG15438   Y Drosophila erecta   Dere\futsch   Y Drosophila yakuba   Dyak\GE16228   Y Drosophila yakuba   Dyak\GE13919   Y Drosophila yakuba   Dyak\GE21749   Y Drosophila yakuba   Dyak\GE18704   Y Drosophila yakuba   Dyak\GE16687   Y Drosophila ananassae   Dana\GF12771   Y Drosophila ananassae   Dana\GF21016   Y Drosophila persimilis   Dper\GL14252   Y Drosophila willistoni   Dwil\GK23126   Y Drosophila willistoni   Dwil\GK15084   Y Drosophila willistoni   Dwil\GK17683   Y Drosophila willistoni   Dwil\GK16856   Y Drosophila willistoni   Dwil\GK24846   Y Drosophila willistoni   Dwil\GK16278   Y Drosophila willistoni   Dwil\GK17163   Y Drosophila willistoni   Dwil\GK23239   Y Drosophila willistoni   Dwil\GK15277   Y Drosophila virilis   Dvir\GJ15230   Y Drosophila mojavensis   Dmoj\GI15698   Y Drosophila grimshawi   Dgri\GH22172   Y Drosophila grimshawi   Dgri\GH10947   Y Drosophila grimshawi   Dgri\GH14949   Y Drosophila grimshawi   Dgri\GH11381   Y Drosophila grimshawi   Dgri\GH19116   Y Drosophila grimshawi   Dgri\GH21846   Y Drosophila grimshawi   Dgri\GH24882   Y
Orthologs in non-Drosophila Dipterans (EOG6D26Z5)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL011181 Y Aedes aegypti Yellow fever mosquito  Aaeg\AAEL009847 Y Aedes aegypti Yellow fever mosquito  Aaeg\AAEL007042 Y Anopheles gambiae Malaria mosquito  Agam\AGAP007155 Y Anopheles gambiae Malaria mosquito  Agam\AGAP000869 Y Anopheles gambiae Malaria mosquito  Agam\AGAP001194 Y Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ013059 Y
Orthologs in non-Dipteran Insects (EOG62V7B2)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB49223 Y Apis mellifera Western honey bee  Amel\GB48862 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG019950 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG011333 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG000493 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH13728 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH14789 Y Atta cephalotes Leafcutter ant  Acep\ACEP23131 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO13277 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL12623 Y Linepithema humile Argentine ant  Lhum\LH26236 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB27254 Y Solenopsis invicta Red fire ant  Sinv\SINV22270 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI000252 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI073896 Y Bombyx mori Silkmoth  Bmor\BGIBMGA007208 Y Pediculus humanus Human body louse  Phum\PHUM399550 Y Tribolium castaneum Red flour beetle  Tcas\TC001001 Y
Orthologs in non-Insect Arthropods (EOG6QBZM4)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Ixodes scapularis Deer tick  Isca\ISCW009767 Y
Orthologs in non-Arthropod Metazoa (EOG6W45DM)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\F32A7.5 Y Caenorhabditis elegans Nematode  Cele\C36A4.5 Y Danio rerio Zebrafish  Drer\map1aa Y
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD16392 Drosophila sechellia Dsec\GM16428 Drosophila erecta Dere\GG11864 Drosophila yakuba Dyak\GE23314 Drosophila ananassae Dana\GF23262 Drosophila pseudoobscura pseudoobscura Dpse\GA11046 Drosophila persimilis Dper\GL23889 Drosophila willistoni Dwil\GK11378 Drosophila virilis Dvir\GJ24130 Drosophila mojavensis Dmoj\GI24202 Drosophila grimshawi Dgri\GH18944
Stocks & Reagents
Stocks Listed in FlyBase ( 9 )
Bloomington	29049 w*; P{UAS-pygo.HA}3/TM6B, Tb1 29720 w*; pygo10/TM6B, Tb1 38208 y1 sc* v1; P{TRiP.GL00647}attP40 7208 w*; P{neoFRT}82B pygoS28/TM3, Sb1 Ser1 7209 w*; P{neoFRT}82B pygoS123/TM6B, P{Ubi-GFP.S65T}PAD2, Tb1
Kyoto	123912 w1118; P{RS3}pygoCB-6097-3
VDRC	v100724 P{KK108114}VIE-260B v19692 w1118; P{GD1516}v19692 v19693 w1118; P{GD1516}v19693
Genomic Clones ( 2 )
	BACR10O09 BACR16C04 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 59 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD18280
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT01724 GH25362 GH28687 LD21971 RE21684 RE45211 RE46758
Other clones	30206 58988 64594 154500 163074 214567.26 214569.24 CK01.76C.H1 CK01.95D.A10 CK01.100C.B6 CK01.108B.C2 CK01.108B.G2 CK01.119A.H11 CK01.121C.E7 CK01.133D.G12 Dmel_neb_014041_0632_1263 EC32728 EC051383 EC052466 EK059822 EK074526 EK084939 EK088602 EK090232 EK090930 EK092341 EK092920 EK138614 EK182521 EK182902 EK258325 EP13324 EP17036 LD16979 RP001006207 RP001009631 RP001012869 RP001014522 RP001016183 RP001020040 RP001023491 RP001025759 RP001030133 RP001030319 RP001038618 RP001042192 RP001045048 RP001049045 RP001049411 RP001051525 RP001054774
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0043900 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43718
Antibody Information
	polyclonal (de la Roche and Bienz, 2007)
Other Information
Discoverer
Etymology
	A pygopus is a legless lizard with scaly skin. (Kramps et al., 2002)
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: pygo gam (Cadigan et al., 2002.3.13)
Source for database merge of	Source for merge of: BEST:LD21971 CG11518 (Bayraktaroglu, 2000.11.2) Source for merge of: gam BEST:LD21971 (Cadigan, 2002.1.24) Source for merge of: pygo anon-WO02077023.1 (FlyBase, 1996-)
Additional comments	FlyBase curator comment: "EP3408" overexpression phenotype stated to be due to its effect on pygo but orientation of the P{EP} element suggests otherwise. (Singh et al., 2005) Source for merge of pygo anon-WO02077023.1 was sequence comparison (date:051113). (FlyBase, 1996-)
Other Comments
	dsRNA has been made from templates generated with primers directed against this gene. RNAi of pygo causes a reduction in the arborization of ddaD and ddaE neurons. There is a severe reduction in primary branch outgrowth, lateral branching and lateral branch length. Additionally, there is also inappropriate dendritic routing of ddaE neurons. (Parrish et al., 2006) dsRNA made from templates generated with primers directed against this gene. (Hoffmans et al., 2005, Fang et al., 2006) pygo is necessary for apoptosis of excess photoreceptors at the periphery of the developing eye during pupal stages. (Lin et al., 2004) In order to activate pan protein, arm protein must enter the nucleus and form a complex with lgs and pygo proteins. (Thompson, 2004) Null mutations have been isolated that affect the epidermis, denticle belts, antenna, legs and wings and are recessive viable. (Cadigan, 2002.1.24) pygo and lgs are required for wg signal transduction at the level of nuclear arm. (Kramps et al., 2002) pygo is required for wg signalling. (Parker et al., 2002) Identification: in a screen to identify P{EP} lines that suppress the small eye phenotype caused by overexpression of wg when coexpressed in the eye. (Cadigan et al., 2001) pygo inhibits wg signalling when overexpressed and when reduced in activity. (Cadigan et al., 2001) Identification: transcription unit identified during molecular analysis of the rod genomic region. (Scaerou et al., 1999)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_143615 RefSeq (Proteins) • NP_651872 DDBJ / EMBL / GenBank • AA538694 AA817007 AAF57161 AAL13729 AAL79357 AAL91369 AF457206 AI405379 AQ025168 AW942476 AX566017 AY058500 AY075095 CZ479557 Entrez Gene - A searchable database of RefSeq genes. • 43718 UniProtKB/Swiss-Prot • Q9V9W8
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Zinc finger, PHD-type (IPR001965) Zinc finger, FYVE/PHD-type (IPR011011) Zinc finger, PHD-type, conserved site (IPR019786) Zinc finger, PHD-finger (IPR019787) Zinc finger, RING/FYVE/PHD-type (IPR013083)
Linkouts
	BioGRID - A database of protein and genetic interactions • 68561 DroID - A comprehensive database of gene and protein interactions. • FBgn0043900 DRSC - Results from RNAi screens. • FBgn0043900 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0043900 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG11518-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0043900 FlyReactome - A curated knowledgebase of Drosophila melanogaster pathways • 99372 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43718 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /segment/pygopus1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43718 modMine - Data generated by the modENCODE project. • FBgn0043900 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q9V9W8
Synonyms & Secondary IDs ( 16 )
Reported As
Symbol Synonym	anon-WO02077023.1   BEST:LD21971 (Abdelilah-Seyfried et al., 2000, ) CG11518 (Tseng and Hariharan, 2002, Bienz, 2006, Keleman et al., 2009.8.5, Ni et al., 2010.12.1) Dm Pygo (Konikoff et al., 2010) dPygo (Kessler et al., 2009) gam (Kramps et al., 2002, Cadigan, 2002.1.24, Cadigan et al., 2001, ) pNB5.2 (Scaerou et al., 1999) pyg (Parrish et al., 2006) pygo (Thompson, 2004, DasGupta et al., 2005, Mosimann et al., 2006, Fang et al., 2006, Bienz, 2006, Jordan et al., 2005, Joulia et al., 2006, Jordan et al., 2006, Ma et al., 2006, Staedeli and Basler, 2005, Cordero and Cagan, 2010, Fiedler et al., 2008, Parker et al., 2008, Kiger et al., 2007, Toku et al., 2011, de la Roche and Bienz, 2007, Grieder et al., 2008, Benchabane et al., 2008, Lebreton et al., 2008, Mieszczanek et al., 2008, Kessler et al., 2009, DasGupta et al., 2007, Japanese National Institute of Genetics, 2012.5.21) Pygo (Parker and Cadigan, 2005, Stadeli, 2006, Benchabane et al., 2011, Benchabane et al., 2011, Carrera et al., 2008, Wright and Tjian, 2009, Bhambhani et al., 2011, Buechling et al., 2011) pypo (Furlong, 2005) unnamed (Tseng and Hariharan, 2001)
Name Synonym	gammy legs (Cadigan et al., 2001) pygopous (Grieder et al., 2008) pygopus (Kramps, 2001.12.11, Cadigan et al., 2002.3.13, Parker, 2002.1.24, Budde, 2006, Parrish et al., 2006, Fang et al., 2006, Jordan et al., 2005, Pfleger et al., 2007, Ma et al., 2006, Cordero and Cagan, 2010, Parker et al., 2008, Lebreton et al., 2008) Pygopus (He and Axelrod, 2006, Clevers, 2006, Bienz, 2006, Tountas and Fortini, 2007, Staedeli and Basler, 2005, Fiedler et al., 2008, de la Roche and Bienz, 2007, Carrera et al., 2008, Wright and Tjian, 2009, Kessler et al., 2009, Bhambhani et al., 2011)
Secondary FlyBase IDs
	FBgn0028951 FBgn0039853 FBgn0064198
References ( 91 )
Generate a list of	All references relating to this gene ( 91 )
List References by type	Research paper  ( 44 ) Supplementary material  ( 5 ) Review  ( 11 ) Personal communication to FlyBase  ( 11 ) Abstract  ( 9 ) FlyBase analysis  ( 3 ) DNA/RNA sequence record  ( 2 ) Conference report  ( 4 ) Stock list  ( 1 ) Patent  ( 1 )
Recent research papers ( 4 )
	Benchabane et al., 2011, EMBO J. 30(8): 1444--1458 Jerky/Earthbound facilitates cell-specific Wnt/Wingless signalling by modulating β-catenin-TCF activity. [FBrf0213544] Bhambhani et al., 2011, EMBO J. 30(10): 2031--2043 The oligomeric state of CtBP determines its role as a transcriptional co-activator and co-repressor of Wingless targets. [FBrf0213805] Buechling et al., 2011, EMBO Rep. 12(12): 1265--1272 p24 proteins are required for secretion of Wnt ligands. [FBrf0216831] Toku et al., 2011, Comput. Biol. Chem. 35(5): 282--292 Reconstruction and crosstalk of protein-protein interaction networks of Wnt and Hedgehog signaling in Drosophila melanogaster. [FBrf0216393] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...