General Information
Symbol
Dmel\Ilp5
Species
D. melanogaster
Name
Insulin-like peptide 5
Annotation Symbol
CG33273
Feature Type
FlyBase ID
FBgn0044048
Gene Model Status
Stock Availability
Gene Snapshot
In progress.Contributions welcome.
Also Known As
dilp5, DILP, dilp-5, Dilp 5, Ilp-5
Genomic Location
Cytogenetic map
Sequence location
3L:9,823,349..9,823,897 [-]
Recombination map
3-31
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
GO Summary Ribbons
Families, Domains and Molecular Function
Protein Family (UniProt, Sequence Similarities)
Belongs to the insulin family. (Q7KUD5)
Molecular Function (see GO section for details)
Summaries
Gene Group Membership
NEUROPEPTIDES -
Neuropeptides are secreted into the extracellular space where they interact with cell surface receptors (usually G protein coupled receptors). They are extremely diverse, acting as neurotransmitters, neuromodulators, hormones or growth factors. (Adapted from FBrf0211443 and PMID:27813667).
INSULIN-LIKE PEPTIDES -
The insulin superfamily consists of peptides such as vertebrate insulins, mammalian relaxin and insulin-like growth factors, and invertebrate insulin-like peptides (ILP). They are synthesized as pre-propeptides and processed to yield two peptides, A and B, linked by disulfide bonds. ILPs have been identified throughout invertebrate species. In D.mel, ILPs may function as peptide hormones and/or neuropeptides. They have been linked to the regulation of growth and development. (Adapted from FBrf0189697 and FBrf0152330).
Insulin-like Receptor Signaling Pathway Core Components -
The Insulin-like Receptor (IR) signaling pathway in Drosophila is initiated by the binding of an insulin-like peptides (ILPs) to the Insulin-like receptor (InR). The core components of the IR signaling pathway are functional in the activated pathway (Adapted from FBrf0232297, FBrf0230017 and FBrf0229989).
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\Ilp5 or the JBrowse view of Dmel\Ilp5 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Gene model reviewed during 5.45
Gene model reviewed during 6.16
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0472645
478
108
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0422627
12.0
108
7.80
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)
Heterodimer of a B chain and an A chain linked by two disulfide bonds.
(UniProt, Q7KUD5)
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ilp5 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (12 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from electronic annotation with InterPro:IPR016179
(assigned by InterPro )
inferred from sequence or structural similarity with UniProtKB:Q9VT51
(assigned by UniProt )
Biological Process (7 terms)
Terms Based on Experimental Evidence (7 terms)
CV Term
Evidence
References
inferred from direct assay
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from sequence or structural similarity with UniProtKB:Q9VT51
(assigned by UniProt )
Cellular Component (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
inferred from direct assay
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from sequence or structural similarity with HGNC:6081
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
RT-PCR
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Expression of Ilp5 in dorsomedial neurosecretory cells increases in first instar larvae and remains high in second and third instar larvae.
Ilp5 is expressed in a bilaterally symmetrical cluster of 5 to 7 neurosecretory cells in the pars intercerebralis. It is co-expressed with Ilp3 and Ilp2.
Transcript is expressed in two clusters of cells in the pars intercerebralis corresponding to the medial neurosecretory cell clusters (m-NSCs) from the mid-late third larval instar.
Transcript is detected at high levels in seven cells in each brain hemisphere in an anterior-medial postion and at low levels in the gut.
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Ilp5 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 3 )
For All Classical and Insertion Alleles Show
 
Allele of Ilp5
Class
Mutagen
Associated Insertion
Stocks
Known lesion
Other relevant insertions
Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Ilp5
Allele of Ilp5
Mutagen
Associated Transgenic Construct
Stocks
Transgenic constructs containing regulatory region of Ilp5
GAL4 construct
Name
Expression Data
reporter construct
Name
Expression Data
Deletions and Duplications ( 2 )
Phenotypes
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
3 of 15
Yes
Yes
 
2 of 15
No
Yes
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
4 of 15
Yes
Yes
3 of 15
No
Yes
Rattus norvegicus (Norway rat) (2)
4 of 13
Yes
Yes
4 of 13
Yes
Yes
Xenopus tropicalis (Western clawed frog) (1)
3 of 12
Yes
Yes
Danio rerio (Zebrafish) (4)
5 of 15
Yes
Yes
5 of 15
Yes
Yes
1 of 15
No
Yes
1 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
3 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (0)
No orthologs reported.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No orthologs reported.
Schizosaccharomyces pombe (Fission yeast) (0)
No orthologs reported.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190K7A )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150I8L )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0SG3 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Tribolium castaneum
Red flour beetle
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0H2F )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )
No non-Arthropod Metazoa orthologies identified
Human Disease Model Data
FlyBase Human Disease Model Reports
    Alleles Reported to Model Human Disease (Disease Ontology)
    Download
    Models ( 1 )
    Allele
    Disease
    Evidence
    References
    model of  sleep disorder
    inferred from mutant phenotype
    Interactions ( 0 )
    Allele
    Disease
    Interaction
    References
    Comments ( 1 )
     
    Ilp51 results in sleep defects when Ilp2 and Ilp3 are also removed using the deficiency Df(3L)Ilp2-3.
    Human Orthologs (via DIOPT v7.1)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Subunit Structure (UniProtKB)
    Heterodimer of a B chain and an A chain linked by two disulfide bonds.
    (UniProt, Q7KUD5 )
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    Pathways
    Gene Group - Pathway Membership (FlyBase)
    Insulin-like Receptor Signaling Pathway Core Components -
    The Insulin-like Receptor (IR) signaling pathway in Drosophila is initiated by the binding of an insulin-like peptides (ILPs) to the Insulin-like receptor (InR). The core components of the IR signaling pathway are functional in the activated pathway (Adapted from FBrf0232297, FBrf0230017 and FBrf0229989).
    External Data
    Linkouts
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    SignaLink - A signaling pathway resource with multi-layered regulatory networks.
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    3L
    Recombination map
    3-31
    Cytogenetic map
    Sequence location
    3L:9,823,349..9,823,897 [-]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    67C9-67C9
    Limits computationally determined from genome sequence between P{PZ}fry02240 and P{lacW}l(3)L0539L0539&P{PZ}Dhh1rL562 67C9
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    Experimentally Determined Recombination Data
    Location
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (14)
    Genomic Clones (16)
     
    cDNA Clones (2)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    BDGP DGC clones
      Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
        Other clones
          RNAi and Array Information
          Linkouts
          DRSC - Results frm RNAi screens
          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
          Antibody Information
          Laboratory Generated Antibodies
          Commercially Available Antibodies
           
          Other Information
          Relationship to Other Genes
          Source for database identify of
          Source for database merge of
          Additional comments
          Other Comments
          New annotation (CG33273) in release 3.2 of the genome annotation.
          Origin and Etymology
          Discoverer
          Etymology
          Identification
          External Crossreferences and Linkouts ( 30 )
          Sequence Crossreferences
          NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
          GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
          RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
          UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
          Other crossreferences
          Linkouts
          Drosophila Genomics Resource Center - Drosophila Genomics Resource Center cDNA clones
          DroID - A comprehensive database of gene and protein interactions.
          DRSC - Results frm RNAi screens
          Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
          FLIGHT - Cell culture data for RNAi and other high-throughput technologies
          FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
          FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
          Flygut - An atlas of the Drosophila adult midgut
          FlyMine - An integrated database for Drosophila genomics
          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
          iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
          InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
          KEGG Genes - Molecular building blocks of life in the genomic space.
          MIST (protein-protein) - An integrated Molecular Interaction Database
          modMine - A data warehouse for the modENCODE project
          Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
          SignaLink - A signaling pathway resource with multi-layered regulatory networks.
          Synonyms and Secondary IDs (28)
          Reported As
          Symbol Synonym
          dilp5
          (Galagovsky et al., 2018, Lin et al., 2018, Semaniuk et al., 2018, Kang et al., 2017, Liao et al., 2017, Schoofs et al., 2017, Zandveld et al., 2017, Hallier et al., 2016, Kubrak et al., 2016, Kučerová et al., 2016, Liu et al., 2016, Schiesari et al., 2016, Wang et al., 2016, Ismail et al., 2015, Kawasaki et al., 2015, Matsuda et al., 2015, Pasco et al., 2015, Sano et al., 2015, Yan et al., 2015, Gündner et al., 2014, Piper et al., 2014, Whitaker et al., 2014, Guirao-Rico and Aguadé, 2013, Hur et al., 2013, Kannan and Fridell, 2013, Karpac et al., 2013, Parisi et al., 2013, Yamamoto et al., 2013, Bai et al., 2012, Banerjee et al., 2012, Kayashima et al., 2012, Mann et al., 2012, Marshall et al., 2012, Rideout et al., 2012, Yu et al., 2012, Alic et al., 2011, Guirao-Rico and Aguadé, 2011, Karpac et al., 2011, McClure et al., 2011, Partridge et al., 2011, Sheldon et al., 2011, Slack et al., 2011, Söderberg et al., 2011, Wigby et al., 2011, De Luca et al., 2010, Grönke et al., 2010, Haselton et al., 2010, Sekine et al., 2010, Slack et al., 2010, Fridell et al., 2009, Hull-Thompson et al., 2009, Humphrey et al., 2009, Karpac et al., 2009, Okamoto et al., 2009, Zhang et al., 2009, Broughton et al., 2008, Buch et al., 2008, Clements et al., 2008, Flatt et al., 2008, Wang et al., 2008, Giannakou et al., 2007, Libert, 2007, Mattaliano et al., 2007, Min et al., 2007, Broughton et al., 2005, Flatt et al., 2005, Tatar et al., 2003, Ikeya and Hafen, 2002, Ikeya et al., 2002, Rulifson et al., 2002, Brogiolo et al., 2001)
          Name Synonyms
          Drosophila insulin-like peptide 5
          Insulin-like peptide
          Insulin-like peptide 5
          insulin like peptide 5
          insulin-like peptide
          insulin-like peptide 5
          insulin-like peptide-5
          Secondary FlyBase IDs
          • FBgn0053273
          Datasets (0)
          Study focus (0)
          Experimental Role
          Project
          Project Type
          Title
          References (244)