Open Close
General Information
Symbol
Dmel\Ilp5
Species
D. melanogaster
Name
Insulin-like peptide 5
Annotation Symbol
CG33273
Feature Type
FlyBase ID
FBgn0044048
Gene Model Status
Stock Availability
Gene Snapshot
Insulin-like peptide 5 (Ilp5) encodes a peptide involved in the insulin signaling pathway, sleep and mating behavior in females. [Date last reviewed: 2019-07-11]
Also Known As
dilp5, DILP, dilp-5, Dilp 5, Ilp-5
Key Links
Genomic Location
Cytogenetic map
Sequence location
3L:9,823,349..9,823,897 [-]
Recombination map
3-31
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Protein Family (UniProt)
Belongs to the insulin family. (Q7KUD5)
Summaries
Gene Group (FlyBase)
NEUROPEPTIDES -
Neuropeptides are secreted into the extracellular space where they interact with cell surface receptors (usually G protein coupled receptors). They are extremely diverse, acting as neurotransmitters, neuromodulators, hormones or growth factors. (Adapted from FBrf0211443 and PMID:27813667).
INSULIN-LIKE PEPTIDES -
The insulin superfamily consists of peptides such as vertebrate insulins, mammalian relaxin and insulin-like growth factors, and invertebrate insulin-like peptides (ILP). They are synthesized as pre-propeptides and processed to yield two peptides, A and B, linked by disulfide bonds. ILPs have been identified throughout invertebrate species. In D.mel, ILPs may function as peptide hormones and/or neuropeptides. They have been linked to the regulation of growth and development. (Adapted from FBrf0189697 and FBrf0152330).
Pathway (FlyBase)
Insulin-like Receptor Signaling Pathway Core Components -
The Insulin-like Receptor (IR) signaling pathway in Drosophila is initiated by the binding of an insulin-like peptides to the Insulin-like receptor (InR). (Adapted from FBrf0232297, FBrf0230017 and FBrf0229989.)
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\Ilp5 or the JBrowse view of Dmel\Ilp5 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Gene model reviewed during 5.45
Gene model reviewed during 6.16
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0472645
478
108
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0422627
12.0
108
7.80
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)
Heterodimer of a B chain and an A chain linked by two disulfide bonds.
(UniProt, Q7KUD5)
Crossreferences
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ilp5 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (12 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from electronic annotation with InterPro:IPR016179
(assigned by InterPro )
inferred from sequence or structural similarity with UniProtKB:Q9VT51
(assigned by UniProt )
Biological Process (7 terms)
Terms Based on Experimental Evidence (7 terms)
CV Term
Evidence
References
inferred from direct assay
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from sequence or structural similarity with UniProtKB:Q9VT51
(assigned by UniProt )
Cellular Component (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
inferred from direct assay
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from sequence or structural similarity with HGNC:6081
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
RT-PCR
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Expression of Ilp5 in dorsomedial neurosecretory cells increases in first instar larvae and remains high in second and third instar larvae.
Ilp5 is expressed in a bilaterally symmetrical cluster of 5 to 7 neurosecretory cells in the pars intercerebralis. It is co-expressed with Ilp3 and Ilp2.
Transcript is expressed in two clusters of cells in the pars intercerebralis corresponding to the medial neurosecretory cell clusters (m-NSCs) from the mid-late third larval instar.
Transcript is detected at high levels in seven cells in each brain hemisphere in an anterior-medial postion and at low levels in the gut.
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Ilp5 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 3 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Ilp5
Transgenic constructs containing regulatory region of Ilp5
Deletions and Duplications ( 2 )
Phenotypes
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
3 of 15
Yes
Yes
 
2 of 15
No
Yes
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
4 of 15
Yes
Yes
3 of 15
No
Yes
Rattus norvegicus (Norway rat) (2)
4 of 13
Yes
Yes
4 of 13
Yes
Yes
Xenopus tropicalis (Western clawed frog) (1)
3 of 12
Yes
Yes
Danio rerio (Zebrafish) (4)
5 of 15
Yes
Yes
5 of 15
Yes
Yes
1 of 15
No
Yes
1 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
3 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190K7A )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150I8L )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0SG3 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Tribolium castaneum
Red flour beetle
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0H2F )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )
No non-Arthropod Metazoa orthologies identified
Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (3)
2 of 10
1 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Allele
Disease
Evidence
References
Potential Models Based on Orthology ( 3 )
Modifiers Based on Experimental Evidence ( 0 )
Allele
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 1 )
 
Ilp51 results in sleep defects when Ilp2 and Ilp3 are also removed using the deficiency Df(3L)Ilp2-3.
Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
Summary of Genetic Interactions
esyN Network Diagram
esyN Network Key:
Suppression
Enhancement

Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
External Data
Subunit Structure (UniProtKB)
Heterodimer of a B chain and an A chain linked by two disulfide bonds.
(UniProt, Q7KUD5 )
Linkouts
DroID - A comprehensive database of gene and protein interactions.
InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
MIST (protein-protein) - An integrated Molecular Interaction Database
Pathways
Gene Group - Pathway Membership (FlyBase)
Insulin-like Receptor Signaling Pathway Core Components -
The Insulin-like Receptor (IR) signaling pathway in Drosophila is initiated by the binding of an insulin-like peptides to the Insulin-like receptor (InR). (Adapted from FBrf0232297, FBrf0230017 and FBrf0229989.)
External Data
Linkouts
Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
SignaLink - A signaling pathway resource with multi-layered regulatory networks.
Genomic Location and Detailed Mapping Data
Chromosome (arm)
3L
Recombination map
3-31
Cytogenetic map
Sequence location
3L:9,823,349..9,823,897 [-]
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
67C9-67C9
Limits computationally determined from genome sequence between P{PZ}fry02240 and P{lacW}l(3)L0539L0539&P{PZ}Dhh1rL562 67C9
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (14)
Genomic Clones (16)
 

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (2)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences
BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    BDGP DGC clones
      Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        Antibody Information
        Laboratory Generated Antibodies
        Commercially Available Antibodies
         
        Other Information
        Relationship to Other Genes
        Source for database identify of
        Source for database merge of
        Additional comments
        Other Comments
        New annotation (CG33273) in release 3.2 of the genome annotation.
        Origin and Etymology
        Discoverer
        Etymology
        Identification
        External Crossreferences and Linkouts ( 34 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
        Other crossreferences
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
        Flygut - An atlas of the Drosophila adult midgut
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
        KEGG Genes - Molecular building blocks of life in the genomic space.
        KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
        modMine - A data warehouse for the modENCODE project
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        SignaLink - A signaling pathway resource with multi-layered regulatory networks.
        Linkouts
        DroID - A comprehensive database of gene and protein interactions.
        DRSC - Results frm RNAi screens
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        FlyMine - An integrated database for Drosophila genomics
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        Synonyms and Secondary IDs (28)
        Reported As
        Symbol Synonym
        dilp5
        (Trinh et al., 2019, Zheng et al., 2019, Galagovsky et al., 2018, Henstridge et al., 2018, Lin et al., 2018, Semaniuk et al., 2018, Kang et al., 2017, Liao et al., 2017, Schoofs et al., 2017, Zandveld et al., 2017, Hallier et al., 2016, Kubrak et al., 2016, Kučerová et al., 2016, Liu et al., 2016, Schiesari et al., 2016, Wang et al., 2016, Ismail et al., 2015, Kawasaki et al., 2015, Li and Gong, 2015, Matsuda et al., 2015, Pasco et al., 2015, Sano et al., 2015, Yan et al., 2015, Gündner et al., 2014, Piper et al., 2014, Whitaker et al., 2014, Guirao-Rico and Aguadé, 2013, Hur et al., 2013, Kannan and Fridell, 2013, Karpac et al., 2013, Parisi et al., 2013, Yamamoto et al., 2013, Bai et al., 2012, Banerjee et al., 2012, Kayashima et al., 2012, Mann et al., 2012, Marshall et al., 2012, Rideout et al., 2012, Yu et al., 2012, Alic et al., 2011, Guirao-Rico and Aguadé, 2011, Karpac et al., 2011, McClure et al., 2011, Partridge et al., 2011, Sheldon et al., 2011, Slack et al., 2011, Söderberg et al., 2011, Wigby et al., 2011, De Luca et al., 2010, Grönke et al., 2010, Haselton et al., 2010, Sekine et al., 2010, Slack et al., 2010, Fridell et al., 2009, Hull-Thompson et al., 2009, Humphrey et al., 2009, Karpac et al., 2009, Okamoto et al., 2009, Zhang et al., 2009, Broughton et al., 2008, Buch et al., 2008, Clements et al., 2008, Flatt et al., 2008, Wang et al., 2008, Giannakou et al., 2007, Libert, 2007, Mattaliano et al., 2007, Min et al., 2007, Broughton et al., 2005, Flatt et al., 2005, Tatar et al., 2003, Ikeya and Hafen, 2002, Ikeya et al., 2002, Rulifson et al., 2002, Brogiolo et al., 2001)
        Name Synonyms
        Drosophila insulin-like peptide 5
        Insulin-like peptide
        Insulin-like peptide 5
        insulin like peptide 5
        insulin-like peptide
        insulin-like peptide 5
        insulin-like peptide-5
        Secondary FlyBase IDs
        • FBgn0053273
        Datasets (0)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        References (265)