General Information
Symbol
Dmel\Ilp4
Species
D. melanogaster
Name
Insulin-like peptide 4
Annotation Symbol
CG6736
Feature Type
FlyBase ID
FBgn0044049
Gene Model Status
Stock Availability
Gene Snapshot
In progress.Contributions welcome.
Also Known As
dilp4, mes3, DILP 4, dilp-4, anon- EST:CLB3
Genomic Location
Cytogenetic map
Sequence location
3L:9,803,399..9,804,263 [-]
Recombination map
3-31
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
GO Summary Ribbons
Families, Domains and Molecular Function
Gene Group Membership (FlyBase)
Protein Family (UniProt, Sequence Similarities)
Belongs to the insulin family. (Q9VT53)
Molecular Function (see GO section for details)
Experimental Evidence
-
Predictions / Assertions
Summaries
Gene Group Membership
NEUROPEPTIDES -
Neuropeptides are secreted into the extracellular space where they interact with cell surface receptors (usually G protein coupled receptors). They are extremely diverse, acting as neurotransmitters, neuromodulators, hormones or growth factors. (Adapted from FBrf0211443 and PMID:27813667).
INSULIN-LIKE PEPTIDES -
The insulin superfamily consists of peptides such as vertebrate insulins, mammalian relaxin and insulin-like growth factors, and invertebrate insulin-like peptides (ILP). They are synthesized as pre-propeptides and processed to yield two peptides, A and B, linked by disulfide bonds. ILPs have been identified throughout invertebrate species. In D.mel, ILPs may function as peptide hormones and/or neuropeptides. They have been linked to the regulation of growth and development. (Adapted from FBrf0189697 and FBrf0152330).
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\Ilp4 or the JBrowse view of Dmel\Ilp4 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.45
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0076372
804
134
Additional Transcript Data and Comments
Reported size (kB)
1 (northern blot)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0076101
15.1
134
9.45
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)
Heterodimer of a B chain and an A chain linked by two disulfide bonds.
(UniProt, Q9VT53)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ilp4 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (7 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000823966
(assigned by GO_Central )
inferred from electronic annotation with InterPro:IPR016179
(assigned by InterPro )
inferred from sequence or structural similarity with HGNC:6081
Biological Process (3 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
inferred from mutant phenotype
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000823966
(assigned by GO_Central )
inferred from sequence or structural similarity with HGNC:6081
Cellular Component (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000823966
(assigned by GO_Central )
inferred from sequence or structural similarity with HGNC:6081
Expression Data
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
mesoderm | ventral

Comment: ventral furrow region

anterior endoderm anlage

Comment: anlage in statu nascendi

dorsal ectoderm anlage

Comment: anlage in statu nascendi

endoderm anlage

Comment: anlage in statu nascendi

head mesoderm anlage

Comment: anlage in statu nascendi

mesoderm anlage

Comment: anlage in statu nascendi

trunk mesoderm anlage

Comment: anlage in statu nascendi

antennal anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

dorsal head epidermis anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

visual anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

Additional Descriptive Data
Transcript is detected in the embryo with high levels in the embryonic mesoderm prior to gastrulation and lower signal after stage 12 and in the anterior midgut rudiment. During the third larval instar transcript is expressed in the midgut.
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
mesoderm | ventral

Comment: ventral furrow region

Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
Reporter: P{Ilp4-lacZ.260}
Stage
Tissue/Position (including subcellular localization)
Reference
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Ilp4 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
Alleles, Insertions, Transgenic Constructs and Phenotypes
Classical and Insertion Alleles ( 1 )
For All Classical and Insertion Alleles Show
 
Allele of Ilp4
Class
Mutagen
Associated Insertion
Stocks
Known lesion
Other relevant insertions
Transgenic Constructs ( 7 )
Deletions and Duplications ( 4 )
Summary of Phenotypes
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
2 of 15
Yes
Yes
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
3 of 15
Yes
Yes
Rattus norvegicus (Norway rat) (1)
4 of 13
Yes
Yes
Xenopus tropicalis (Western clawed frog) (0)
No orthologs reported.
Danio rerio (Zebrafish) (2)
2 of 15
Yes
Yes
1 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (0)
No orthologs reported.
Arabidopsis thaliana (thale-cress) (0)
No orthologs reported.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No orthologs reported.
Schizosaccharomyces pombe (Fission yeast) (0)
No orthologs reported.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190KIL )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150J0R )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Lucilia cuprina
Australian sheep blowfly
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( None identified )
No non-Dipteran orthologies identified
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0PUF )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Stegodyphus mimosarum
African social velvet spider
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )
No non-Arthropod Metazoa orthologies identified
Human Disease Model Data
FlyBase Human Disease Model Reports
    Alleles Reported to Model Human Disease (Disease Ontology)
    Download
    Models ( 0 )
    Allele
    Disease
    Evidence
    References
    Interactions ( 0 )
    Allele
    Disease
    Interaction
    References
    Comments ( 0 )
     
    Human Orthologs (via DIOPT v7.1)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser

    Please look at the Interaction Group reports for full details of the physical interactions
    protein-protein
    Interacting group
    Assay
    References
    Summary of Genetic Interactions
    esyN Network Diagram
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Subunit Structure (UniProtKB)
    Heterodimer of a B chain and an A chain linked by two disulfide bonds.
    (UniProt, Q9VT53 )
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    Pathways
    Gene Group - Pathway Membership (FlyBase)
    External Data
    Linkouts
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    SignaLink - A signaling pathway resource with multi-layered regulatory networks.
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    3L
    Recombination map
    3-31
    Cytogenetic map
    Sequence location
    3L:9,803,399..9,804,263 [-]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    67C8-67C8
    Limits computationally determined from genome sequence between P{PZ}fry02240 and P{lacW}l(3)L0539L0539&P{PZ}Dhh1rL562
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    67D-67E
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Location
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (6)
    Genomic Clones (20)
    cDNA Clones (44)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    BDGP DGC clones
    Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      RNAi and Array Information
      Linkouts
      DRSC - Results frm RNAi screens
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      Antibody Information
      Laboratory Generated Antibodies
       
      Commercially Available Antibodies
       
      Other Information
      Relationship to Other Genes
      Source for database identify of
      Source for database merge of
      Source for merge of: Ilp4 CG6736
      Source for merge of: Ilp4 anon- EST:CLB3
      Source for merge of: Ilp4 BcDNA:RE27042
      Additional comments
      Source for merge of Ilp4 anon- EST:CLB3 was sequence comparison ( date:040624 ).
      Source for merge of Ilp4 BcDNA:RE27042 was a shared cDNA ( date:030728 ).
      Other Comments
      Isolated from a subtractive cDNA library enriched in sequences expressed in the mesoderm.
      An Ilp4 cDNA has been cloned and sequenced. Ilp4 is expressed in the early mesoderm.
      Origin and Etymology
      Discoverer
      Etymology
      Identification
      External Crossreferences and Linkouts ( 35 )
      Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
      UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
      Other crossreferences
      BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
      InterPro - A database of protein families, domains and functional sites
      Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
      Linkouts
      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center cDNA clones
      DroID - A comprehensive database of gene and protein interactions.
      DRSC - Results frm RNAi screens
      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
      FLIGHT - Cell culture data for RNAi and other high-throughput technologies
      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Flygut - An atlas of the Drosophila adult midgut
      FlyMine - An integrated database for Drosophila genomics
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      KEGG Genes - Molecular building blocks of life in the genomic space.
      modMine - A data warehouse for the modENCODE project
      SignaLink - A signaling pathway resource with multi-layered regulatory networks.
      Synonyms and Secondary IDs (29)
      Reported As
      Symbol Synonym
      BcDNA:RE27042
      anon-EST:CLB3
      Name Synonyms
      Drosophila insulin-like peptide 4
      Drosophila insulin/relaxin-like peptide
      Insulin-like peptide 4
      insulin-like peptide
      insulin-like peptide 4
      insulin-like peptide-4
      Secondary FlyBase IDs
      • FBgn0019856
      • FBgn0036048
      • FBgn0047136
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      References (103)