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Gene Dmel\Cka

General Information
Symbol	Dmel\Cka	Species	D. melanogaster
Name	Connector of kinase to AP-1	Annotation symbol	CG7392
Feature type	protein_coding_gene	FlyBase ID	FBgn0044323
Gene Model Status	Current	Stock availability	10 publicly available
Also Known As	l(2)05836
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	28D3-28D3	Sequence location	2L:8,030,721..8,040,826 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Connector of kinase to AP-1 is referred to in FlyBase by the symbol Dmel\Cka (CG7392, FBgn0044323). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding. There is experimental evidence that it is involved in the biological process: dorsal closure; compound eye photoreceptor development; positive regulation of JNK cascade; phagocytosis, engulfment. 16 alleles are reported. The phenotypes of these alleles are annotated with: embryonic/first instar larval cuticle; imaginal disc; head. It has 7 annotated transcripts and 7 annotated polypeptides. Protein features are: G-protein beta WD-40 repeat; Striatin, N-terminal; WD40 repeat; WD40 repeat, conserved site; WD40-repeat-containing domain; WD40/YVTN repeat-like-containing domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-18 hour embryonic stages, during early pupal stages, in adult male stages. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval fat body, adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1637815_s_at completely aligns to an exonic region common to each of the 4 FlyBase-annotated transcript isoforms of Cka. Gene sequence location is 2L:8030721..8040826.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03	Controlled Vocabulary Terms regulation of cell cycle
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 28D3-28D3   Limits computationally determined from genome sequence between P{lacW}l(2)k10210k10210 and P{EP}poeEP737
Experimentally Determined Cytological Location
Cytogenetic map Notes References 28E-28E     (Hou, 2001.3.13) 28E1-28E2   (determined by in situ hybridisation)   (Spradling et al., 1999) 28E1-28E2   (determined by in situ hybridisation)   (Perrimon et al., 1996) 28D8-28D11     (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Cka for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.46 Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated Annotated transcripts do not represent all supported alternative splices within 5' UTR. Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Cka-RA FBtr0079536  NM_135333   3274   730 Cka-RB FBtr0079537  NM_164779   5135   730 Cka-RC FBtr0079539  NM_164780   3254   730 Cka-RD FBtr0079538  NM_164781   3003   730 Cka-RE FBtr0306000  NM_001259007   2819   749 Cka-RF FBtr0306001  NM_001259008   5366   747 Cka-RG FBtr0333096  NM_001273292   2738   722
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Cka-PA   FBpp0079158   78.0   730   4.86     NP_609177   AAF52593 Cka-PB   FBpp0079159   78.0   730   4.86     NP_723334   AAF52594 Cka-PC   FBpp0079161   78.0   730   4.86     NP_723335   AAN10651 Cka-PD   FBpp0079160   78.0   730   4.86     NP_723336   AAN10652 Cka-PE   FBpp0297142   80.1   749   4.68     NP_001245936   AFH03610 Cka-PF   FBpp0297143   79.9   747   4.71     NP_001245937   AFH03611 Cka-PG   FBpp0305309   77.1   722   4.71     NP_001260221   AGB92757
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • WD40 repeat (IPR001680) Striatin, N-terminal (IPR013258) WD40-repeat-containing domain (IPR017986) WD40/YVTN repeat-like-containing domain (IPR015943) WD40 repeat, conserved site (IPR019775) G-protein beta WD-40 repeat (IPR020472)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA202703   AA202878   AA390641   AA390731   AA440748   AA441572   AA694798   AA695470   AA817507   AA941575   AA942467   AA948968   AA950264   AE014134 AAF52593   AE014134 AAF52594   AE014134 AAN10651   AE014134 AAN10652   AF011332 AAD01441   AF364305 AAK48946   AE014134 AFH03610   AE014134 AFH03611   AE014134 AGB92757   AI063565   AI107694   AI109336   AI257223   AI257608   AI258275   AI260252   AI260764   AI260826   AI401912   AI455033   AI512346   AI512721   AI515382   AI516026   AI516328   AI516610   AI518360   AI518454   AI520179   AI531103   AI532641   AI533746   AI533784   AQ025643   AQ073650   AX260212   AX260213   AX260215   AX260217   AY061460 AAL29008   BE976905   BF485868   BF486167   BF491556   BF491735   BF496254   BF496555   BF502070   BF503265   BF505830   BG639035   BI141941   BI167008   BI168173   BI168706   BI215529   BI234955   BI239187   BI239950   BI241985   BI242863   BI243081   BI356395   BI357326   BI362164   BI363349   BI363362   BI364021   BI364756   BI364932   BI364945   BI369842   BI371405   BI482131   BI563893   BI563987   BI565462   BI566671   BI567627   BI567984   BI568173   BI575331   BI577801   BI588700   BI613403   BI614751   BI615356   BI619443   BI622779   BI627321   BI630520   BI631426   BI638252   BI640840   BI641845   BP556676   BP556677   BT120179   BX048442   CK657059   CK657901   CK659227   CK660898   CL844365   CO156893   CO179834   CO188976   CO266554   CO280060   CO286098   CO304758   CO313280   CO313486   CO322617   CO329108   CO330730   CO331518   CO332933   CO335937   EC057764   EC065260   EC067537   EC069891   EC077842   EC090498   EC209962   EC216411   EC220437   EC221962   EC222181   EC225589   EC228266   EC235035   EC238695   EC242072   EC249049   EC266813   EC267663   EV582098   GH747901   GH748021   GH748723   GH750130   GH751541   GH751624   GH752419   GH752635   GH755340   GH755835   GH755972   GH757144   GH757787   GH759312   GH759979   GH761027   GH770130   GH770150   GH770980   GH772275   GH774675   GH776096   GH776344   GH777044   GH777165   GH777782   GH780516   GH780662   GH780673   GH783621   GH783807   GH784304   GH784893   GH784948   GH785166   GH787450   GH788333   GH788499   GH790156   GH792623   GH792993   GH793576   GH793808   GH794540   GH795172   GH796388   GH797054   GH797259   GH798121   GH799572   GH799611   GH800308   GH800865   GH801406   GH801991   GH802249   GH803304   GH803491   GH803933   GH804513   GH805427   GH805898   GH806726   GH806879   GH807950   GH807999   GH809666   GH809978   GH810166   GH811936   GH813759   GH814378   GH816846   GH817120   GH819091   GH820270   GH821380   GH822182   GH826458   GH826773   GH830561   GH834195   GH835235   GH836690   GH836825   GH837346   GH837555   GH839298   GH839302   GH841126   GH841270   GH843643   GH843692   GH844145   GH844288   GH844829   GH845592   GH846328   GH846442   GH846840   GH847239   GH847274   GH848105   GH848513   GH853044   GH854487   GH855621   GH856526   GH858589   GH859544   GH860031   GH861501   GH862616   GH863065   GH863227   GH864160   GH865352   GH865476   GH866622   GH867274   GH868434   GH869157   GH869599   GH870466   GH871007   GH871653   GH874075   GH874962   GH876928   GH878165   GH878267   GH878541   GH878657   GH879778   GH882764   GH882939   GH883230   GH883357   GH884449   GH886563   GH886968   GH887498   GH888018   GH888030   GH889285   GH889775   GH889898   GH889969   GH890990   GH891003   GH894545   GH895501   GH895761   GH896292   GH898751   GH900643   GH900888   GH900956   GH902508   GH903048   GH903055   GH903633   GH904060   GH904838   GH905944   GH906156   GH906251   GH906531   GH906763   GH907241   GH907313   GH909186   GH909858   GH910085   GH910350   GH911844   GH912206   GH912942   GH916079   GH916601   GH916814   GH917011   GH917622   GH919202   GH919324   GH919647   GH920790   GH923507   GH924243   GH924747   GH926473   GH927122   GH928062   GH929737   GH931111   GH931675   GH932772   GH934013   GH934139   GH935633   GH936306   GH936762   GH937833   GH940848
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VLT9 Q86M30
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism | ubiquitous   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 13 -- 16   organism | ubiquitous   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
western blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage -- larval stage   organism   (Weng and Du, 2002)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
cytoplasm inferred from direct assay (Chen et al., 2002) mitotic spindle inferred from direct assay (Müller et al., 2010) nucleus inferred from direct assay (Chen et al., 2002)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval fat body, adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    125.275 Larval Midgut    58 Larval Hindgut    121.4 Larval Malpighian Tubules    219 Larval Fat Body    149.4 Larval Salivary Gland    65.2 Larval Trachea    420.675 Larval Carcass    54.05 Adult Head    178.9 Adult Eye    392.125 Adult Brain    421.2 Adult Thoracic-Abdominal Ganglion    363 Adult Crop    222.8 Adult Midgut    157.1 Adult Hindgut    231.6 Adult Malpighian Tubules    218.2 Adult Fat Body    87.2 Adult Salivary Gland    109.4 Adult Heart    342.15 Adult VirginFemale Spermatheca    213.6 Adult InseminatedFemale Spermatheca    234.7 Adult Ovary    240.1 Adult Testis    380.7 Adult Male Accessory Gland    172.7 Adult Carcass    173.7 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    125.275 Larval Midgut    58 Larval Hindgut    121.4 Larval Malpighian Tubules    219 Larval Fat Body    149.4 Larval Salivary Gland    65.2 Larval Trachea    420.675 Larval Carcass    54.05 Adult Head    178.9 Adult Eye    392.125 Adult Brain    421.2 Adult Thoracic-Abdominal Ganglion    363 Adult Crop    222.8 Adult Midgut    157.1 Adult Hindgut    231.6 Adult Malpighian Tubules    218.2 Adult Fat Body    87.2 Adult Salivary Gland    109.4 Adult Heart    342.15 Adult VirginFemale Spermatheca    213.6 Adult InseminatedFemale Spermatheca    234.7 Adult Ovary    240.1 Adult Testis    380.7 Adult Male Accessory Gland    172.7 Adult Carcass    173.7 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    125.275 Larval Midgut    58 Larval Hindgut    121.4 Larval Malpighian Tubules    219 Larval Fat Body    149.4 Larval Salivary Gland    65.2 Larval Trachea    420.675 Larval Carcass    54.05 Adult Head    178.9 Adult Eye    392.125 Adult Brain    421.2 Adult Thoracic-Abdominal Ganglion    363 Adult Crop    222.8 Adult Midgut    157.1 Adult Hindgut    231.6 Adult Malpighian Tubules    218.2 Adult Fat Body    87.2 Adult Salivary Gland    109.4 Adult Heart    342.15 Adult VirginFemale Spermatheca    213.6 Adult InseminatedFemale Spermatheca    234.7 Adult Ovary    240.1 Adult Testis    380.7 Adult Male Accessory Gland    172.7 Adult Carcass    173.7 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    125.275 Larval Midgut    58 Larval Hindgut    121.4 Larval Malpighian Tubules    219 Larval Fat Body    149.4 Larval Salivary Gland    65.2 Larval Trachea    420.675 Larval Carcass    54.05 Adult Head    178.9 Adult Eye    392.125 Adult Brain    421.2 Adult Thoracic-Abdominal Ganglion    363 Adult Crop    222.8 Adult Midgut    157.1 Adult Hindgut    231.6 Adult Malpighian Tubules    218.2 Adult Fat Body    87.2 Adult Salivary Gland    109.4 Adult Heart    342.15 Adult VirginFemale Spermatheca    213.6 Adult InseminatedFemale Spermatheca    234.7 Adult Ovary    240.1 Adult Testis    380.7 Adult Male Accessory Gland    172.7 Adult Carcass    173.7 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    125.275 Larval Midgut    58 Larval Hindgut    121.4 Larval Malpighian Tubules    219 Larval Fat Body    149.4 Larval Salivary Gland    65.2 Larval Trachea    420.675 Larval Carcass    54.05 Adult Head    178.9 Adult Eye    392.125 Adult Brain    421.2 Adult Thoracic-Abdominal Ganglion    363 Adult Crop    222.8 Adult Midgut    157.1 Adult Hindgut    231.6 Adult Malpighian Tubules    218.2 Adult Fat Body    87.2 Adult Salivary Gland    109.4 Adult Heart    342.15 Adult VirginFemale Spermatheca    213.6 Adult InseminatedFemale Spermatheca    234.7 Adult Ovary    240.1 Adult Testis    380.7 Adult Male Accessory Gland    172.7 Adult Carcass    173.7 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    125.275 Larval Midgut    58 Larval Hindgut    121.4 Larval Malpighian Tubules    219 Larval Fat Body    149.4 Larval Salivary Gland    65.2 Larval Trachea    420.675 Larval Carcass    54.05 Adult Head    178.9 Adult Eye    392.125 Adult Brain    421.2 Adult Thoracic-Abdominal Ganglion    363 Adult Crop    222.8 Adult Midgut    157.1 Adult Hindgut    231.6 Adult Malpighian Tubules    218.2 Adult Fat Body    87.2 Adult Salivary Gland    109.4 Adult Heart    342.15 Adult VirginFemale Spermatheca    213.6 Adult InseminatedFemale Spermatheca    234.7 Adult Ovary    240.1 Adult Testis    380.7 Adult Male Accessory Gland    172.7 Adult Carcass    173.7 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    125.275 Larval Midgut    58 Larval Hindgut    121.4 Larval Malpighian Tubules    219 Larval Fat Body    149.4 Larval Salivary Gland    65.2 Larval Trachea    420.675 Larval Carcass    54.05 Adult Head    178.9 Adult Eye    392.125 Adult Brain    421.2 Adult Thoracic-Abdominal Ganglion    363 Adult Crop    222.8 Adult Midgut    157.1 Adult Hindgut    231.6 Adult Malpighian Tubules    218.2 Adult Fat Body    87.2 Adult Salivary Gland    109.4 Adult Heart    342.15 Adult VirginFemale Spermatheca    213.6 Adult InseminatedFemale Spermatheca    234.7 Adult Ovary    240.1 Adult Testis    380.7 Adult Male Accessory Gland    172.7 Adult Carcass    173.7 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    125.275 Larval Midgut    58 Larval Hindgut    121.4 Larval Malpighian Tubules    219 Larval Fat Body    149.4 Larval Salivary Gland    65.2 Larval Trachea    420.675 Larval Carcass    54.05 Adult Head    178.9 Adult Eye    392.125 Adult Brain    421.2 Adult Thoracic-Abdominal Ganglion    363 Adult Crop    222.8 Adult Midgut    157.1 Adult Hindgut    231.6 Adult Malpighian Tubules    218.2 Adult Fat Body    87.2 Adult Salivary Gland    109.4 Adult Heart    342.15 Adult VirginFemale Spermatheca    213.6 Adult InseminatedFemale Spermatheca    234.7 Adult Ovary    240.1 Adult Testis    380.7 Adult Male Accessory Gland    172.7 Adult Carcass    173.7 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    178.9   NA  Eye    392.125   NA  Brain    421.2   125.275  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    363   NA  Crop    222.8   58  Midgut    157.1   121.4  Hindgut    231.6   219  Malpighian Tubules    218.2   149.4  Fat Body    87.2   65.2  Salivary Gland    109.4   NA  Heart    342.15   420.675  Trachea    NA   NA  VirginFemale Spermatheca    213.6   NA  InseminatedFemale Spermatheca    234.7   NA  Ovary    240.1   NA  Testis    380.7   NA  Male Accessory Gland    172.7   54.05  Carcass    173.7 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    63 central nervous system, larvae L3    112 central nervous system, pupae P8    100 head, virgin 1-day female    41 head, virgin 4-day female    39 head, virgin 20-day female    29 head, mated 1-day female    37 head, mated 4-day female    33 head, mated 20-day female    26 head, mated 1-day male    55 head, mated 4-day male    54 head, mated 20-day male    39 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    27 digestive system, larvae L3 wandering    23 digestive system, 1-day adult    19 digestive system, 4-day adult    24 digestive system, 20-day adult    18 fat body, larvae L3 wandering    17 fat body, white prepupae    24 fat body, pupae P8    35 carcass, larvae L3 wandering    49 carcass, 1-day adult    23 carcass, 4-day adult    27 carcass, 20-day adult    23 ovary, virgin 4-day female    33 ovary, mated 4-day female    38 testis, mated 4-day male    53 accessory gland, mated 4-day male    16 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (63) central nervous system, larvae L3  (112) central nervous system, pupae P8  (100) head, virgin 1-day female  (41) head, virgin 4-day female  (39) head, virgin 20-day female  (29) head, mated 1-day female  (37) head, mated 4-day female  (33) head, mated 20-day female  26 head, mated 1-day male  (55) head, mated 4-day male  (54) head, mated 20-day male  (39) salivary gland, larvae L3 wandering    3 salivary gland, white prepupae  (27) digestive system, larvae L3 wandering    23 digestive system, 1-day adult    19 digestive system, 4-day adult    24 digestive system, 20-day adult    18 fat body, larvae L3 wandering    17 fat body, white prepupae    24 fat body, pupae P8  (35) carcass, larvae L3 wandering  (49) carcass, 1-day adult    23 carcass, 4-day adult  (27) carcass, 20-day adult    23 ovary, virgin 4-day female  (33) ovary, mated 4-day female  (38) testis, mated 4-day male  (53) accessory gland, mated 4-day male    16 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    63 central nervous system, larvae L3  (112) central nervous system, pupae P8    100 head, virgin 1-day female    41 head, virgin 4-day female    39 head, virgin 20-day female    29 head, mated 1-day female    37 head, mated 4-day female    33 head, mated 20-day female    26 head, mated 1-day male    55 head, mated 4-day male    54 head, mated 20-day male    39 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    27 digestive system, larvae L3 wandering    23 digestive system, 1-day adult    19 digestive system, 4-day adult    24 digestive system, 20-day adult    18 fat body, larvae L3 wandering    17 fat body, white prepupae    24 fat body, pupae P8    35 carcass, larvae L3 wandering    49 carcass, 1-day adult    23 carcass, 4-day adult    27 carcass, 20-day adult    23 ovary, virgin 4-day female    33 ovary, mated 4-day female    38 testis, mated 4-day male    53 accessory gland, mated 4-day male    16 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    63 central nervous system, larvae L3    112 central nervous system, pupae P8    100 head, virgin 1-day female    41 head, virgin 4-day female    39 head, virgin 20-day female    29 head, mated 1-day female    37 head, mated 4-day female    33 head, mated 20-day female    26 head, mated 1-day male    55 head, mated 4-day male    54 head, mated 20-day male    39 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    27 digestive system, larvae L3 wandering    23 digestive system, 1-day adult    19 digestive system, 4-day adult    24 digestive system, 20-day adult    18 fat body, larvae L3 wandering    17 fat body, white prepupae    24 fat body, pupae P8    35 carcass, larvae L3 wandering    49 carcass, 1-day adult    23 carcass, 4-day adult    27 carcass, 20-day adult    23 ovary, virgin 4-day female    33 ovary, mated 4-day female    38 testis, mated 4-day male    53 accessory gland, mated 4-day male    16 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    63 central nervous system, larvae L3    112 central nervous system, pupae P8    100 head, virgin 1-day female    41 head, virgin 4-day female    39 head, virgin 20-day female    29 head, mated 1-day female    37 head, mated 4-day female    33 head, mated 20-day female    26 head, mated 1-day male    55 head, mated 4-day male    54 head, mated 20-day male    39 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    27 digestive system, larvae L3 wandering    23 digestive system, 1-day adult    19 digestive system, 4-day adult    24 digestive system, 20-day adult    18 fat body, larvae L3 wandering    17 fat body, white prepupae    24 fat body, pupae P8    35 carcass, larvae L3 wandering    49 carcass, 1-day adult    23 carcass, 4-day adult    27 carcass, 20-day adult    23 ovary, virgin 4-day female    33 ovary, mated 4-day female    38 testis, mated 4-day male    53 accessory gland, mated 4-day male    16 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (63) central nervous system, larvae L3  (112) central nervous system, pupae P8  (100) head, virgin 1-day female  (41) head, virgin 4-day female  (39) head, virgin 20-day female  29 head, mated 1-day female  (37) head, mated 4-day female  (33) head, mated 20-day female  26 head, mated 1-day male  (55) head, mated 4-day male  (54) head, mated 20-day male  (39) salivary gland, larvae L3 wandering    3 salivary gland, white prepupae  27 digestive system, larvae L3 wandering    23 digestive system, 1-day adult    19 digestive system, 4-day adult    24 digestive system, 20-day adult    18 fat body, larvae L3 wandering    17 fat body, white prepupae    24 fat body, pupae P8  (35) carcass, larvae L3 wandering  (49) carcass, 1-day adult    23 carcass, 4-day adult  27 carcass, 20-day adult    23 ovary, virgin 4-day female  (33) ovary, mated 4-day female  (38) testis, mated 4-day male  (53) accessory gland, mated 4-day male    16 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    63 central nervous system, larvae L3  112 central nervous system, pupae P8    100 head, virgin 1-day female    41 head, virgin 4-day female    39 head, virgin 20-day female    29 head, mated 1-day female    37 head, mated 4-day female    33 head, mated 20-day female    26 head, mated 1-day male    55 head, mated 4-day male    54 head, mated 20-day male    39 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    27 digestive system, larvae L3 wandering    23 digestive system, 1-day adult    19 digestive system, 4-day adult    24 digestive system, 20-day adult    18 fat body, larvae L3 wandering    17 fat body, white prepupae    24 fat body, pupae P8    35 carcass, larvae L3 wandering    49 carcass, 1-day adult    23 carcass, 4-day adult    27 carcass, 20-day adult    23 ovary, virgin 4-day female    33 ovary, mated 4-day female    38 testis, mated 4-day male    53 accessory gland, mated 4-day male    16 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    63 central nervous system, larvae L3    112 central nervous system, pupae P8    100 head, virgin 1-day female    41 head, virgin 4-day female    39 head, virgin 20-day female    29 head, mated 1-day female    37 head, mated 4-day female    33 head, mated 20-day female    26 head, mated 1-day male    55 head, mated 4-day male    54 head, mated 20-day male    39 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    27 digestive system, larvae L3 wandering    23 digestive system, 1-day adult    19 digestive system, 4-day adult    24 digestive system, 20-day adult    18 fat body, larvae L3 wandering    17 fat body, white prepupae    24 fat body, pupae P8    35 carcass, larvae L3 wandering    49 carcass, 1-day adult    23 carcass, 4-day adult    27 carcass, 20-day adult    23 ovary, virgin 4-day female    33 ovary, mated 4-day female    38 testis, mated 4-day male    53 accessory gland, mated 4-day male    16 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-18 hour embryonic stages, during early pupal stages, in adult male stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    37 embryo 02-04hr    43 embryo 04-06hr    57 embryo 06-08hr    59 embryo 08-10hr    49 embryo 10-12hr    41 embryo 12-14hr    40 embryo 14-16hr    43 embryo 16-18hr    34 embryo 18-20hr    30 embryo 20-22hr    26 embryo 22-24hr    26 larva L1    27 larva L2    16 larva L3 12hr old    13 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    25 larva L3 puffstage 7-9    30 white prepupae new    36 white prepupae 12hr    38 white prepupae 24hr    45 pupae 2d postWPP    37 pupae 3d postWPP    25 pupae 4d postWPP    32 adult male 01day    29 adult male 05day    33 adult male 30day    35 adult female 01day    29 adult female 05day    25 adult female 30day    25 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (37) embryo 02-04hr  (43) embryo 04-06hr  (57) embryo 06-08hr  (59) embryo 08-10hr  (49) embryo 10-12hr  (41) embryo 12-14hr  (40) embryo 14-16hr  (43) embryo 16-18hr  (34) embryo 18-20hr  (30) embryo 20-22hr  26 embryo 22-24hr  26 larva L1  (27) larva L2    16 larva L3 12hr old    13 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    25 larva L3 puffstage 7-9  (30) white prepupae new  (36) white prepupae 12hr  (38) white prepupae 24hr  (45) pupae 2d postWPP  (37) pupae 3d postWPP    25 pupae 4d postWPP  (32) adult male 01day  (29) adult male 05day  (33) adult male 30day  (35) adult female 01day  (29) adult female 05day    25 adult female 30day    25 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    37 embryo 02-04hr    43 embryo 04-06hr    57 embryo 06-08hr    59 embryo 08-10hr    49 embryo 10-12hr    41 embryo 12-14hr    40 embryo 14-16hr    43 embryo 16-18hr    34 embryo 18-20hr    30 embryo 20-22hr    26 embryo 22-24hr    26 larva L1    27 larva L2    16 larva L3 12hr old    13 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    25 larva L3 puffstage 7-9    30 white prepupae new    36 white prepupae 12hr    38 white prepupae 24hr    45 pupae 2d postWPP    37 pupae 3d postWPP    25 pupae 4d postWPP    32 adult male 01day    29 adult male 05day    33 adult male 30day    35 adult female 01day    29 adult female 05day    25 adult female 30day    25 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    37 embryo 02-04hr    43 embryo 04-06hr    57 embryo 06-08hr    59 embryo 08-10hr    49 embryo 10-12hr    41 embryo 12-14hr    40 embryo 14-16hr    43 embryo 16-18hr    34 embryo 18-20hr    30 embryo 20-22hr    26 embryo 22-24hr    26 larva L1    27 larva L2    16 larva L3 12hr old    13 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    25 larva L3 puffstage 7-9    30 white prepupae new    36 white prepupae 12hr    38 white prepupae 24hr    45 pupae 2d postWPP    37 pupae 3d postWPP    25 pupae 4d postWPP    32 adult male 01day    29 adult male 05day    33 adult male 30day    35 adult female 01day    29 adult female 05day    25 adult female 30day    25 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    37 embryo 02-04hr    43 embryo 04-06hr    57 embryo 06-08hr    59 embryo 08-10hr    49 embryo 10-12hr    41 embryo 12-14hr    40 embryo 14-16hr    43 embryo 16-18hr    34 embryo 18-20hr    30 embryo 20-22hr    26 embryo 22-24hr    26 larva L1    27 larva L2    16 larva L3 12hr old    13 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    25 larva L3 puffstage 7-9    30 white prepupae new    36 white prepupae 12hr    38 white prepupae 24hr    45 pupae 2d postWPP    37 pupae 3d postWPP    25 pupae 4d postWPP    32 adult male 01day    29 adult male 05day    33 adult male 30day    35 adult female 01day    29 adult female 05day    25 adult female 30day    25 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (37) embryo 02-04hr  (43) embryo 04-06hr  (57) embryo 06-08hr  (59) embryo 08-10hr  (49) embryo 10-12hr  (41) embryo 12-14hr  (40) embryo 14-16hr  (43) embryo 16-18hr  (34) embryo 18-20hr  30 embryo 20-22hr  26 embryo 22-24hr  26 larva L1  27 larva L2    16 larva L3 12hr old    13 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    25 larva L3 puffstage 7-9  30 white prepupae new  (36) white prepupae 12hr  (38) white prepupae 24hr  (45) pupae 2d postWPP  (37) pupae 3d postWPP    25 pupae 4d postWPP  32 adult male 01day  29 adult male 05day  (33) adult male 30day  (35) adult female 01day  29 adult female 05day    25 adult female 30day    25 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    37 embryo 02-04hr    43 embryo 04-06hr    57 embryo 06-08hr    59 embryo 08-10hr    49 embryo 10-12hr    41 embryo 12-14hr    40 embryo 14-16hr    43 embryo 16-18hr    34 embryo 18-20hr    30 embryo 20-22hr    26 embryo 22-24hr    26 larva L1    27 larva L2    16 larva L3 12hr old    13 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    25 larva L3 puffstage 7-9    30 white prepupae new    36 white prepupae 12hr    38 white prepupae 24hr    45 pupae 2d postWPP    37 pupae 3d postWPP    25 pupae 4d postWPP    32 adult male 01day    29 adult male 05day    33 adult male 30day    35 adult female 01day    29 adult female 05day    25 adult female 30day    25 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    37 embryo 02-04hr    43 embryo 04-06hr    57 embryo 06-08hr    59 embryo 08-10hr    49 embryo 10-12hr    41 embryo 12-14hr    40 embryo 14-16hr    43 embryo 16-18hr    34 embryo 18-20hr    30 embryo 20-22hr    26 embryo 22-24hr    26 larva L1    27 larva L2    16 larva L3 12hr old    13 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    25 larva L3 puffstage 7-9    30 white prepupae new    36 white prepupae 12hr    38 white prepupae 24hr    45 pupae 2d postWPP    37 pupae 3d postWPP    25 pupae 4d postWPP    32 adult male 01day    29 adult male 05day    33 adult male 30day    35 adult female 01day    29 adult female 05day    25 adult female 30day    25 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    64 Schneider line 2 Sg4    39 embryonic 1182-4H    39 embryonic GM2    40 embryonic Kc167    40 embryonic S1    46 embryonic S3    57 leg disc CME L1    29 wing disc CME-W2    26 wing disc ML-DmD8    46 wing disc ML-DmD9    36 wing disc ML-DmD16-c3    75 wing disc ML-DmD21    34 wing disc ML-DmD32    45 haltere disc ML-DmD17-c3    102 eye-antennal disc ML-DmD11    35 antennal disc ML-DmD20-c5    37 mixed discs ML-DmD4-c1    60 CNS ML-DmBG1-c1    56 CNS ML-DmBG2-c2    47 tumorous blood cells mbn2    60 ovary fGS/OSS    53 ovary OSC    45 ovary OSS    31 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (64) Schneider line 2 Sg4  (39) embryonic 1182-4H  (39) embryonic GM2  (40) embryonic Kc167  (40) embryonic S1  (46) embryonic S3  (57) leg disc CME L1  (29) wing disc CME-W2  26 wing disc ML-DmD8  (46) wing disc ML-DmD9  (36) wing disc ML-DmD16-c3  (75) wing disc ML-DmD21  (34) wing disc ML-DmD32  (45) haltere disc ML-DmD17-c3  (102) eye-antennal disc ML-DmD11  (35) antennal disc ML-DmD20-c5  (37) mixed discs ML-DmD4-c1  (60) CNS ML-DmBG1-c1  (56) CNS ML-DmBG2-c2  (47) tumorous blood cells mbn2  (60) ovary fGS/OSS  (53) ovary OSC  (45) ovary OSS  (31) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    64 Schneider line 2 Sg4    39 embryonic 1182-4H    39 embryonic GM2    40 embryonic Kc167    40 embryonic S1    46 embryonic S3    57 leg disc CME L1    29 wing disc CME-W2    26 wing disc ML-DmD8    46 wing disc ML-DmD9    36 wing disc ML-DmD16-c3    75 wing disc ML-DmD21    34 wing disc ML-DmD32    45 haltere disc ML-DmD17-c3  102 eye-antennal disc ML-DmD11    35 antennal disc ML-DmD20-c5    37 mixed discs ML-DmD4-c1    60 CNS ML-DmBG1-c1    56 CNS ML-DmBG2-c2    47 tumorous blood cells mbn2    60 ovary fGS/OSS    53 ovary OSC    45 ovary OSS    31 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    64 Schneider line 2 Sg4    39 embryonic 1182-4H    39 embryonic GM2    40 embryonic Kc167    40 embryonic S1    46 embryonic S3    57 leg disc CME L1    29 wing disc CME-W2    26 wing disc ML-DmD8    46 wing disc ML-DmD9    36 wing disc ML-DmD16-c3    75 wing disc ML-DmD21    34 wing disc ML-DmD32    45 haltere disc ML-DmD17-c3    102 eye-antennal disc ML-DmD11    35 antennal disc ML-DmD20-c5    37 mixed discs ML-DmD4-c1    60 CNS ML-DmBG1-c1    56 CNS ML-DmBG2-c2    47 tumorous blood cells mbn2    60 ovary fGS/OSS    53 ovary OSC    45 ovary OSS    31 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    64 Schneider line 2 Sg4    39 embryonic 1182-4H    39 embryonic GM2    40 embryonic Kc167    40 embryonic S1    46 embryonic S3    57 leg disc CME L1    29 wing disc CME-W2    26 wing disc ML-DmD8    46 wing disc ML-DmD9    36 wing disc ML-DmD16-c3    75 wing disc ML-DmD21    34 wing disc ML-DmD32    45 haltere disc ML-DmD17-c3    102 eye-antennal disc ML-DmD11    35 antennal disc ML-DmD20-c5    37 mixed discs ML-DmD4-c1    60 CNS ML-DmBG1-c1    56 CNS ML-DmBG2-c2    47 tumorous blood cells mbn2    60 ovary fGS/OSS    53 ovary OSC    45 ovary OSS    31 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (64) Schneider line 2 Sg4  (39) embryonic 1182-4H  (39) embryonic GM2  (40) embryonic Kc167  (40) embryonic S1  (46) embryonic S3  (57) leg disc CME L1  29 wing disc CME-W2  26 wing disc ML-DmD8  (46) wing disc ML-DmD9  (36) wing disc ML-DmD16-c3  (75) wing disc ML-DmD21  (34) wing disc ML-DmD32  (45) haltere disc ML-DmD17-c3  (102) eye-antennal disc ML-DmD11  (35) antennal disc ML-DmD20-c5  (37) mixed discs ML-DmD4-c1  (60) CNS ML-DmBG1-c1  (56) CNS ML-DmBG2-c2  (47) tumorous blood cells mbn2  (60) ovary fGS/OSS  (53) ovary OSC  (45) ovary OSS  31 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    64 Schneider line 2 Sg4    39 embryonic 1182-4H    39 embryonic GM2    40 embryonic Kc167    40 embryonic S1    46 embryonic S3    57 leg disc CME L1    29 wing disc CME-W2    26 wing disc ML-DmD8    46 wing disc ML-DmD9    36 wing disc ML-DmD16-c3    75 wing disc ML-DmD21    34 wing disc ML-DmD32    45 haltere disc ML-DmD17-c3  102 eye-antennal disc ML-DmD11    35 antennal disc ML-DmD20-c5    37 mixed discs ML-DmD4-c1    60 CNS ML-DmBG1-c1    56 CNS ML-DmBG2-c2    47 tumorous blood cells mbn2    60 ovary fGS/OSS    53 ovary OSC    45 ovary OSS    31 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    64 Schneider line 2 Sg4    39 embryonic 1182-4H    39 embryonic GM2    40 embryonic Kc167    40 embryonic S1    46 embryonic S3    57 leg disc CME L1    29 wing disc CME-W2    26 wing disc ML-DmD8    46 wing disc ML-DmD9    36 wing disc ML-DmD16-c3    75 wing disc ML-DmD21    34 wing disc ML-DmD32    45 haltere disc ML-DmD17-c3    102 eye-antennal disc ML-DmD11    35 antennal disc ML-DmD20-c5    37 mixed discs ML-DmD4-c1    60 CNS ML-DmBG1-c1    56 CNS ML-DmBG2-c2    47 tumorous blood cells mbn2    60 ovary fGS/OSS    53 ovary OSC    45 ovary OSS    31 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    29 cold shock, 4-day adult    22 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    12 Cadmium 50 mM 12 hrs, larvae L3    14 Cadmium 50 mM 48 hrs, 4-day adult    20 Cadmium 100 mM 48 hrs, 4-day adult    35 Copper 0.5 mM 12 hrs, larvae L3    19 Copper 15 mM 48 hrs, 4-day adult    67 Zinc 5 mM 12 hrs, larvae L3    18 Zinc 4.5 mM 48 hrs, 4-day adult    50 Ethanol 2.5% 3 hrs, larvae L3    32 Ethanol 5% 3 hrs, larvae L3    13 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    12 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    34 Caffeine 25 mg/ml 48 hrs, 4-day adult    26 Paraquat 5 mM 48 hrs, 4-day adult    33 Paraquat 10 mM 48 hrs, 4-day adult    29 Rotenone 2 μg 12 hrs, larvae L3    11 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (29) cold shock, 4-day adult    22 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    12 Cadmium 50 mM 12 hrs, larvae L3    14 Cadmium 50 mM 48 hrs, 4-day adult    20 Cadmium 100 mM 48 hrs, 4-day adult  (35) Copper 0.5 mM 12 hrs, larvae L3    19 Copper 15 mM 48 hrs, 4-day adult  (67) Zinc 5 mM 12 hrs, larvae L3    18 Zinc 4.5 mM 48 hrs, 4-day adult  (50) Ethanol 2.5% 3 hrs, larvae L3  (32) Ethanol 5% 3 hrs, larvae L3    13 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    12 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (34) Caffeine 25 mg/ml 48 hrs, 4-day adult  26 Paraquat 5 mM 48 hrs, 4-day adult  (33) Paraquat 10 mM 48 hrs, 4-day adult  (29) Rotenone 2 μg 12 hrs, larvae L3    11 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    29 cold shock, 4-day adult    22 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    12 Cadmium 50 mM 12 hrs, larvae L3    14 Cadmium 50 mM 48 hrs, 4-day adult    20 Cadmium 100 mM 48 hrs, 4-day adult    35 Copper 0.5 mM 12 hrs, larvae L3    19 Copper 15 mM 48 hrs, 4-day adult    67 Zinc 5 mM 12 hrs, larvae L3    18 Zinc 4.5 mM 48 hrs, 4-day adult    50 Ethanol 2.5% 3 hrs, larvae L3    32 Ethanol 5% 3 hrs, larvae L3    13 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    12 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    34 Caffeine 25 mg/ml 48 hrs, 4-day adult    26 Paraquat 5 mM 48 hrs, 4-day adult    33 Paraquat 10 mM 48 hrs, 4-day adult    29 Rotenone 2 μg 12 hrs, larvae L3    11 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    29 cold shock, 4-day adult    22 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    12 Cadmium 50 mM 12 hrs, larvae L3    14 Cadmium 50 mM 48 hrs, 4-day adult    20 Cadmium 100 mM 48 hrs, 4-day adult    35 Copper 0.5 mM 12 hrs, larvae L3    19 Copper 15 mM 48 hrs, 4-day adult    67 Zinc 5 mM 12 hrs, larvae L3    18 Zinc 4.5 mM 48 hrs, 4-day adult    50 Ethanol 2.5% 3 hrs, larvae L3    32 Ethanol 5% 3 hrs, larvae L3    13 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    12 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    34 Caffeine 25 mg/ml 48 hrs, 4-day adult    26 Paraquat 5 mM 48 hrs, 4-day adult    33 Paraquat 10 mM 48 hrs, 4-day adult    29 Rotenone 2 μg 12 hrs, larvae L3    11 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    29 cold shock, 4-day adult    22 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    12 Cadmium 50 mM 12 hrs, larvae L3    14 Cadmium 50 mM 48 hrs, 4-day adult    20 Cadmium 100 mM 48 hrs, 4-day adult    35 Copper 0.5 mM 12 hrs, larvae L3    19 Copper 15 mM 48 hrs, 4-day adult    67 Zinc 5 mM 12 hrs, larvae L3    18 Zinc 4.5 mM 48 hrs, 4-day adult    50 Ethanol 2.5% 3 hrs, larvae L3    32 Ethanol 5% 3 hrs, larvae L3    13 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    12 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    34 Caffeine 25 mg/ml 48 hrs, 4-day adult    26 Paraquat 5 mM 48 hrs, 4-day adult    33 Paraquat 10 mM 48 hrs, 4-day adult    29 Rotenone 2 μg 12 hrs, larvae L3    11 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  29 cold shock, 4-day adult    22 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    12 Cadmium 50 mM 12 hrs, larvae L3    14 Cadmium 50 mM 48 hrs, 4-day adult    20 Cadmium 100 mM 48 hrs, 4-day adult  (35) Copper 0.5 mM 12 hrs, larvae L3    19 Copper 15 mM 48 hrs, 4-day adult  (67) Zinc 5 mM 12 hrs, larvae L3    18 Zinc 4.5 mM 48 hrs, 4-day adult  (50) Ethanol 2.5% 3 hrs, larvae L3  32 Ethanol 5% 3 hrs, larvae L3    13 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    12 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (34) Caffeine 25 mg/ml 48 hrs, 4-day adult  26 Paraquat 5 mM 48 hrs, 4-day adult  (33) Paraquat 10 mM 48 hrs, 4-day adult  29 Rotenone 2 μg 12 hrs, larvae L3    11 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    29 cold shock, 4-day adult    22 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    12 Cadmium 50 mM 12 hrs, larvae L3    14 Cadmium 50 mM 48 hrs, 4-day adult    20 Cadmium 100 mM 48 hrs, 4-day adult    35 Copper 0.5 mM 12 hrs, larvae L3    19 Copper 15 mM 48 hrs, 4-day adult    67 Zinc 5 mM 12 hrs, larvae L3    18 Zinc 4.5 mM 48 hrs, 4-day adult    50 Ethanol 2.5% 3 hrs, larvae L3    32 Ethanol 5% 3 hrs, larvae L3    13 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    12 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    34 Caffeine 25 mg/ml 48 hrs, 4-day adult    26 Paraquat 5 mM 48 hrs, 4-day adult    33 Paraquat 10 mM 48 hrs, 4-day adult    29 Rotenone 2 μg 12 hrs, larvae L3    11 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    29 cold shock, 4-day adult    22 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    12 Cadmium 50 mM 12 hrs, larvae L3    14 Cadmium 50 mM 48 hrs, 4-day adult    20 Cadmium 100 mM 48 hrs, 4-day adult    35 Copper 0.5 mM 12 hrs, larvae L3    19 Copper 15 mM 48 hrs, 4-day adult    67 Zinc 5 mM 12 hrs, larvae L3    18 Zinc 4.5 mM 48 hrs, 4-day adult    50 Ethanol 2.5% 3 hrs, larvae L3    32 Ethanol 5% 3 hrs, larvae L3    13 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    12 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    34 Caffeine 25 mg/ml 48 hrs, 4-day adult    26 Paraquat 5 mM 48 hrs, 4-day adult    33 Paraquat 10 mM 48 hrs, 4-day adult    29 Rotenone 2 μg 12 hrs, larvae L3    11 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_27 mE1_20_mRNA_expression_cluster_02
External Data & Images
Linkouts	BDGP expression data • LD41760 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0044323 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG7392-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | embryonic stage | fully | rescuable maternal effect Cka05836 lethal | embryonic stage | maternal effect | recessive Cka05836 lethal | pupal stage, with Scer\GAL4pnr-MD237 CkaGD12225 lethal | pupal stage (with Cka05836) Ckas1883 lethal | pupal stage (with Ckas1883) Cka05836 lethal | pupal stage | recessive Cka05836 Ckas1883 lethal | recessive Cka2 Cka3 Cka4 Cka05836 Ckak12603 Ckak12606 Ckas1883 viable CkaEY10818 Sterility Allele fertile CkaEY10818 Phenotype manifest in Allele embryonic/first instar larval cuticle Cka05836 embryonic/first instar larval cuticle | dorsal Cka05836 embryonic/first instar larval cuticle | dorsal | germline clone | rescuable maternal effect Cka05836 embryonic dorsal epidermis | germline clone | maternal effect Cka2 Cka3 embryonic dorsal epidermis | germline clone | rescuable maternal effect Cka05836 embryonic head | germline clone | rescuable maternal effect Cka05836 embryonic leading edge cell | germline clone | maternal effect Cka05836 head Cka05836 imaginal disc Ckak12603 imaginal disc (with Cka05836) Ckas1883 imaginal disc (with Ckas1883) Cka05836
Classical Alleles ( 10 )
For All Classical Alleles Show
Allele of Cka Class Mutagen Stocks Known lesion Cka05836 P-element activity 1 Yes Ckak12603 P-element activity 1 -- Ckak12606 P-element activity 1 -- Ckas1883 P-element activity 1 -- Cka2 Delta2-3 0 Yes Cka3 Delta2-3 0 Yes Cka4 Delta2-3 0 Yes CkaDG29705 P-element activity 0 -- CkaEP2514 P-element activity 0 Yes CkaEY10818 P-element activity 0 --
Alleles Carried on Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Cka Class Mutagen Stocks Known lesion CkaGD12225 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes CkaHM05138 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes CkaHMS00081 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes CkaJF01432 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes CkaKK100338 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Ckahs.PC in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Not disrupted in	Df(2L)TE29Aa-11 (Spradling et al., 1999, BDGP Project Members, 1994-1999) Df(2L)XE-2750 (Spradling et al., 1999, BDGP Project Members, 1994-1999) Df(2L)XE-3801 (BDGP Project Members, 1994-1999)
Disrupted in	Df(2L)baf2 (Furukawa et al., 2003)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD12225} NA P{KK100338} NA P{TRiP.HM05138} NA P{TRiP.HMS00081} NA P{TRiP.JF01432} NA heat-shock construct P{hs-Cka.C} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}CkaEP2514 NA P{EPgy2}CkaEY10818 NA insertion of enhancer trap P{lacW}Ckak12603 No P{lacW}Ckak12606 No P{lacW}Ckas1883 No P{lacW}l(2)SH0824SH0824 No P{PZ}Cka05836 No miscellaneous insertions P{wHy}CkaDG29705 NA
Gene Ontology: Function, Process & Cellular Component ( 10 unique terms )
Terms Based on Experimental Evidence ( 9 terms )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with Jra AND inferred from physical interaction with bsk AND inferred from physical interaction with hep AND inferred from physical interaction with kay (Chen et al., 2002)
Biological Process
CV term Evidence References
compound eye photoreceptor development inferred from mutant phenotype (Weng and Du, 2002) dorsal closure inferred from mutant phenotype (Chen et al., 2002) phagocytosis, engulfment inferred from mutant phenotype (Stroschein-Stevenson et al., 2006) positive regulation of JNK cascade inferred from direct assay (Chen et al., 2002) inferred from genetic interaction with bsk (Chen et al., 2002) inferred from genetic interaction with hep (Chen et al., 2002) inferred from genetic interaction with Jra (Chen et al., 2002) inferred from genetic interaction with kay (Chen et al., 2002) regulation of cell cycle inferred from direct assay (Guest et al., 2011)
Cellular Component
CV term Evidence References
cytoplasm inferred from direct assay (Chen et al., 2002) mitotic spindle inferred from direct assay (Müller et al., 2010) nucleus inferred from direct assay (Chen et al., 2002)
Terms Based on Predictions or Assertions ( 2 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
dorsal closure non-traceable author statement (Chen et al., 2001) traceable author statement (Harden, 2002) JNK cascade non-traceable author statement (Chen et al., 2001, Melnick, 1997.6.26) traceable author statement (Harden, 2002)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Cka - CG10158 anti tag coimmunoprecipitation, peptide massfingerprinting, anti tag western blot (Ribeiro et al., 2010) Cka - CG10915 anti tag coimmunoprecipitation, peptide massfingerprinting (Ribeiro et al., 2010) Cka - CG11526 anti tag coimmunoprecipitation, peptide massfingerprinting (Ribeiro et al., 2010) Cka - CG1246 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cka - GckIII anti tag coimmunoprecipitation, peptide massfingerprinting (Ribeiro et al., 2010) Cka - Mob4 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23, Ribeiro et al., 2010) Cka - Pp2A-29B anti tag coimmunoprecipitation, peptide massfingerprinting (Ribeiro et al., 2010) Cka - Rassf anti tag coimmunoprecipitation, anti tag western blot, peptide massfingerprinting (Ribeiro et al., 2010) Cka - hpo anti tag coimmunoprecipitation, anti tag western blot, peptide massfingerprinting (Ribeiro et al., 2010) Cka - msn anti tag coimmunoprecipitation, peptide massfingerprinting (Ribeiro et al., 2010) Cka - mts anti tag coimmunoprecipitation, peptide massfingerprinting (Ribeiro et al., 2010)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Cka allele Gene References Cka2 hep (Chen et al., 2002) Cka05836 bsk (Chen et al., 2002) hep (Chen et al., 2002) Jra (Chen et al., 2002) kay (Chen et al., 2002) tkv (Chen et al., 2002)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 60230 DroID - A comprehensive database of gene and protein interactions. • FBgn0044323 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 34096 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q86M30
Orthologs
OrthoDB Orthologs (48) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG65TD6P Dipteran inclusive ortholog search EOG6933S1 Insect inclusive ortholog search EOG6W0W85 Arthropod inclusive ortholog search EOG600008 Metazoa inclusive ortholog search EOG6N93XP
Orthologs in Drosophila Species (EOG65TD6P)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Cka     Drosophila simulans   Dsim\GD23499 Y   Drosophila sechellia   Dsec\GM16687 Y   Drosophila erecta   Dere\GG10508 Y   Drosophila yakuba   Dyak\GE18729 Y   Drosophila ananassae   Dana\GF14574 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA20319 Y   Drosophila persimilis   Dper\GL18738 Y   Drosophila willistoni   Dwil\GK24370 Y   Drosophila virilis   Dvir\GJ17687 Y   Drosophila mojavensis   Dmoj\GI17915 Y   Drosophila grimshawi   Dgri\GH11611 Y
Orthologs in non-Drosophila Dipterans (EOG6933S1)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL015320   Anopheles gambiae Malaria mosquito  Agam\AGAP008755   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ003087
Orthologs in non-Dipteran Insects (EOG6W0W85)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB48367   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG002460   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH13945   Atta cephalotes Leafcutter ant  Acep\ACEP16260   Camponotus floridanus Florida carpenter ant  Cflo\CFLO17738   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL20826   Linepithema humile Argentine ant  Lhum\LH22320   Pogonomyrmex barbatus Red harvester ant  Pbar\PB19596   Solenopsis invicta Red fire ant  Sinv\SINV19420   Acyrthosiphon pisum Pea aphid  Apim\ACYPI004457   Bombyx mori Silkmoth  Bmor\BGIBMGA010562   Pediculus humanus Human body louse  Phum\PHUM241320   Tribolium castaneum Red flour beetle  Tcas\GLEAN_07565
Orthologs in non-Insect Arthropods (EOG600008)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_208467   Ixodes scapularis Deer tick  Isca\ISCW012130
Orthologs in non-Arthropod Metazoa (EOG6N93XP)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\cash-1   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_004519gn   Danio rerio Zebrafish  Drer\strn3   Danio rerio Zebrafish  Drer\strn4   Danio rerio Zebrafish  Drer\ENSDARG00000060595   Xenopus tropicalis Western clawed frog  Xtro\strn   Xenopus tropicalis Western clawed frog  Xtro\strn3   Gallus gallus Domestic chicken  Ggal\STRN   Gallus gallus Domestic chicken  Ggal\ENSGALG00000009930   Mus musculus House mouse  Mmus\Strn4   Mus musculus House mouse  Mmus\Strn   Mus musculus House mouse  Mmus\Strn3   Rattus norvegicus Norway rat  Rnor\Strn3   Rattus norvegicus Norway rat  Rnor\Strn4   Rattus norvegicus Norway rat  Rnor\Strn   Homo sapiens Human  Hsap\STRN4   Homo sapiens Human  Hsap\STRN   Homo sapiens Human  Hsap\ENSG00000196792
Human Orthologs (3)
Gene OMIM HGNC Hsap\STRN4     Hsap\STRN     Hsap\ENSG00000196792
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD23499 Drosophila sechellia Dsec\GM16687 Drosophila erecta Dere\GG10508 Drosophila yakuba Dyak\GE18729 Drosophila ananassae Dana\GF14574 Drosophila pseudoobscura pseudoobscura Dpse\GA20319 Drosophila persimilis Dper\GL18738 Drosophila willistoni Dwil\GK24370 Drosophila virilis Dvir\GJ17687 Drosophila mojavensis Dmoj\GI17915 Drosophila grimshawi Dgri\GH11611
Stocks & Reagents
Stocks Listed in FlyBase ( 10 )
Bloomington	12039 w1118; P{lacW}Ckas1883/CyO 28927 y1 v1; P{TRiP.HM05138}attP2 31642 y1 v1; P{TRiP.JF01432}attP2 11451 P{PZ}Cka05836 cn1/CyO; ry506 34522 y1 sc* v1; P{TRiP.HMS00081}attP2
Kyoto	102839 y1 w67c23 ; P{lacW}Ckak12603 / CyO 102840 y1 w67c23 ; P{lacW}Ckak12606 / CyO
VDRC	v106971 P{KK100338}VIE-260B v35232 w1118; P{GD12225}v35232 v35234 w1118; P{GD12225}v35234
Genomic Clones ( 1 )
	BACR08I01 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 335 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD41760
Other clones	MIP15828
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT08055 AT10015 AT10327 AT17688 AT19215 AT19937 AT20378 AT28323 AT28544 GH05516 GH08487 GM01563 GM02877 LD03193 LD03224 LD04963 LD08849 LD08865 LD15621 LD16551 LD23147 LD25774 LD26706 LD27684 LD28758 LD37849 LD37971 LD40250 LD42534 LD44191 LD44689 LD47187 LP01425 LP03975 LP04733 LP04813 LP05529 LP06021 LP16845 LP19850 LP23039 RE07275 RE08745 RE09551 RE22052 RE29136 RE30734 RE35612 RE36580 RE39194 RE40326 RE40631 RE42531 RE43773 RE46657 RE48134 RE48148 RE49046 RE49933 RE50134 RE50148 RE56157 RE58283 RE64782 RE71549 RH30105 RH30641 RH34626 RH34952 RH36380 RH38164 RH39306 RH39560 RH42552 RH44214 RH44963 RH50477 RH54696 RH59581 RH60995 RH63238 RH68602 SD02011 SD04222 SD05704 SD05745 SD15919 SD20494 SD23849 SD25488
Other clones	16901 35945 39366 62853 106267 107273 122956 158822 164908 193342 194299 273054 280614 293161 296250 bs57e04 CK01.48D.G11 CK01.79C.H3 CK01.87C.E2 CK01.168D.G12 Dmel_dig_003746_0720_0741 EC00301 EK042626 EK061730 EK146835 EK152329 EK188514 EK232057 EK233050 EK246054 EK292038 EK296039 EK300035 EK305164 EN09519 EN11524 FGM304H07 GH03445 LD42160 RP003001374 RP003002087 RP003010530 RP003011578 RP003011733 RP003021673 RP003021989 RP003026822 RP003027172 RP003032454 RP003036142 RP003038547 RP003040551 RP003042648 RP003054301 RP003054799 RP003055218 RP003062696 RP003064108 RP003064202 RP003071790 RP003073042 RP003073252 RP003074713 RP003077267 RP003078486 RP003079730 RP003084802 RP003089144 RP003091633 RP003102033 RP003104406 RP003107448 RP003108769 RP003113314 RP003114950 RP003115033 RP003117025 RP003118120 RP003118926 RP003125529 RP003129236 RP003139475 RP003139893 RP003145729 RP003149577 RP003150080 RP003150165 RP003150692 RP003153915 RP003158203 RP003161373 RP003161523 RP003163999 RP003164062 RP003172810 RP003176586 RP003177613 RP003185979 RP003186186 RP003186560 RP003186675 RP003189340 RP003191140 RP003192176 RP003197143 RP003199858 RP003203185 RP003204120 RP003207018 RP003207285 RP003209683 RP003214569 RP003221289 RP003222189 RP003225369 RP003225728 RP003228352 RP003229975 RP003233944 RP003242129 RP003245631 RP003248228 RP003250034 RP003250050 RP003251040 RP003252375 RP003252841 RP003260513 RP003261792 RP003265491 RP003268243 RP003268462 RP003273586 RP003276900 RP003276931 RP003279813 RP003282992 RP003284313 RP003286184 RP003290171 RP003304579 RP003308390 RP003309428 RP003319040 RP003323340 RP003327722 RP003328286 RP003340400 RP003349745 RP003350287 RP003354337 RP003356402 RP003360334 RP003373815 RP003375986 RP003379245 RP003381280 RP003389130 RP003393144 RP003398820 RP003403790 RP003405689 RP003409677 RP003421366 RP003425882 RP003448567 RP003449515 RP003453671 RP003455342 RP003456584 RP003457015 RP003458833 RP003462022 RP003465806 RP003466428 RP003468641 RP003471134 RP003472946 RP003479200 RP003481443 RP003481971 RP003484573 RP003495568 RP003510596 RP003514056 RP003516562 RP003517300 RP003517311 RP003518716 RP003527082 RP003528609 RP003529204 RP003530062 RP003530233 RP003536094 RP003539539 RP003541455 RP003544020 RP003546250 RP003548648 RP003551135 RP003551217 RP003553041 RP003553998 RP003562527 RP003564652 RP003569430 RP003569872 RP003580761 RP003583874 RP003585141 RP003588236 RP003588629 RP003591774 RP003592227 RP003594360 RP003597895 RP003600731 RP003601280 RP003612418 RP003616378 RP003621171 RP003621619 RP003622162 RP003624843 RP003627665 RP003633131 RP003634599 RP003634705 RP003637106 RP003637909 RP003640350 RP003641583 RP003646391 RP003647420 RP003648588 RP003650705 RP003650843 RP003653650 RP003653979 RP003656909 RP003674746 RP003677335
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0044323 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 34096
Antibody Information
	polyclonal (Weng and Du, 2002)
Other Information
Discoverer
Etymology
	The gene is named "connector of kinase to AP-1" based on its presumed role in the JNK signal transduction pathway. (Chen et al., 2002)
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: Cka anon-WO0172774.174 anon-WO0172774.175 anon-WO0172774.177 anon-WO0172774.179 (FlyBase, 1996-) Source for merge of: Cka WD-40-family-member (FlyBase, 1996-) Source for merge of: WD-40-family-member CG7392 (Bayraktaroglu, 2000.4.12) Source for merge of: WD-40-family-member l(2)05836 (Misra, 2000.11.18)
Additional comments	Source for merge of Cka anon-WO0172774.174 anon-WO0172774.175 anon-WO0172774.177 anon-WO0172774.179 was sequence comparison (date:051113). (FlyBase, 1996-) Source for merge of Cka WD-40-family-member was sequence comparison (date:020313). (FlyBase, 1996-)
Other Comments
	S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells. (Stroschein-Stevenson et al., 2006) dsRNA made from templates generated with primers directed against Cka that is transfected into S2 treated with Listeria monocytogenes reveals Cka to be involved in Listeria monocytogenes entry. (Cheng et al., 2005) Cka controls the localized expression of dpp in the leading edge cells. (Chen et al., 2001) Cka functions in the JNK dorsal closure pathway. (Chen et al., 2001) Identification: Enhancer trap expression pattern survey for loci expressed in the ring gland. (Harvie et al., 1998) Mutants isolated in a screen of the second chromosome identifying genes affecting disc morphology. (Roch et al., 1998) The autosomal "FLP-DFS" technique (using the P{ovoD1-18} P{FRT(whs)} P{hsFLP} chromosomes) has been used to identify the specific maternal effect phenotype for the zygotic lethal mutation. (Perrimon et al., 1996)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_135333 NM_164779 NM_164780 NM_164781 NM_001259007 NM_001259008 NM_001273292 RefSeq (Proteins) • NP_609177 NP_723334 NP_723335 NP_723336 NP_001245936 NP_001245937 NP_001260221 DDBJ / EMBL / GenBank • AAD01441 AAF52593 AAF52594 AAK48946 AAL29008 AAN10651 AAN10652 AF011332 AF364305 AFH03610 AFH03611 AGB92757 AI516026 AQ025643 AQ073650 AX260212 AX260213 AX260215 AX260217 AY061460 CL844365 Entrez Gene - A searchable database of RefSeq genes. • 34096 UniProtKB/TrEMBL • Q9VLT9 Q86M30
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • WD40 repeat (IPR001680) Striatin, N-terminal (IPR013258) WD40-repeat-containing domain (IPR017986) WD40/YVTN repeat-like-containing domain (IPR015943) WD40 repeat, conserved site (IPR019775) G-protein beta WD-40 repeat (IPR020472) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD41760
Linkouts
	BioGRID - A database of protein and genetic interactions • 60230 DroID - A comprehensive database of gene and protein interactions. • FBgn0044323 DRSC - Results from RNAi screens. • FBgn0044323 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0044323 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG7392-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0044323 FlyReactome - A curated knowledgebase of Drosophila melanogaster pathways • 205634 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 34096 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 34096 modMine - Data generated by the modENCODE project. • FBgn0044323 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q86M30
Synonyms & Secondary IDs ( 15 )
Reported As
Symbol Synonym	5836 (Harvie et al., 1998) anon-WO0172774.174   anon-WO0172774.175   anon-WO0172774.177   anon-WO0172774.179   CG7392 (Melnick, 1997.6.26, Anholt and Mackay, 2001, Mount, 2001.8.14, Konduri and Beckingham, 2006, Konduri and Beckingham, 2007, Keleman et al., 2009.8.5, Perkins et al., 2009, Ni et al., 2008, Ni et al., 2011.7.19) cka (Chen et al., 2002, Zhang et al., 2010, Ribeiro et al., 2010) CKA (Weng and Du, 2002, Yang et al., 2003, Chen et al., 2001) Cka (Furukawa et al., 2003, Stroschein-Stevenson et al., 2006, Herranz et al., 2010, Guest et al., 2011, Bond and Foley, 2009, Zhou et al., 2008, Müller et al., 2010) Dcka (Oh, 2001.3.26) l(2)05836 (Spradling et al., 1999, Russell et al., 1998, Roch et al., 1998, Perrimon et al., 1996, BDGP Project Members, 1994-1999, ) l(2)s1883
Name Synonym	connector of kinase to AP-1 (Chen et al., 2002) Connector of kinase to AP-1 (Chen et al., 2001, Ribeiro et al., 2010) WD-40-family-member (Misra, 2000.11.18, )
Secondary FlyBase IDs
	FBgn0010608 FBgn0010681 FBgn0026012 FBgn0031978 FBgn0046423 FBgn0046425 FBgn0046426 FBgn0046427
References ( 56 )
Generate a list of	All references relating to this gene ( 56 )
List References by type	Research paper  ( 20 ) Supplementary material  ( 6 ) Review  ( 3 ) Personal communication to FlyBase  ( 16 ) Abstract  ( 3 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 2 ) Website  ( 1 ) Patent  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 2 )
	Guest et al., 2011, BMC Syst. Biol. 5(1): 65 A protein network-guided screen for cell cycle regulators in Drosophila. [FBrf0213913] Horn et al., 2011, Nat. Methods 8(4): 341--346 Mapping of signaling networks through synthetic genetic interaction analysis by RNAi. [FBrf0213352] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...