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Gene Dmel\Pak3

General Information
Symbol	Dmel\Pak3	Species	D. melanogaster
Name	Pak3	Annotation symbol	CG14895
Feature type	protein_coding_gene	FlyBase ID	FBgn0044826
Gene Model Status	Current	Stock availability	14 publicly available
Also Known As	DPAK3, DmPAK3
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	89B16-89B17	Sequence location	3R:12,270,729..12,279,550 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Pak3 is referred to in FlyBase by the symbol Dmel\Pak3 (CG14895, FBgn0044826). It is a protein_coding_gene from Drosophila melanogaster. It is reported to have molecular function: receptor signaling protein serine/threonine kinase activity. There is experimental evidence that it is involved in the biological process: actin filament organization. 17 alleles are reported. The phenotypes of these alleles are annotated with: trichogen cell; embryonic/larval cuticle. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: PAK-box/P21-Rho-binding; Protein kinase, ATP binding site; Protein kinase, catalytic domain; Protein kinase-like domain; Serine/threonine-protein kinase, active site; Serine/threonine-protein kinase, catalytic domain; Serine/threonine-protein kinase-like domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: larval/adult midgut. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1628149_a_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Pak3. Gene sequence location is 3R:12270729..12279550.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10	Transgenic Constructs P{UAS-Pak3.GFP}
FB2012_01	Sequence features BKN51184 BKN31386 BKN50615 BKN45897 HFA15017
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 89B16-89B17   Limits computationally determined from genome sequence between P{PZ}gish04895&P{EP}EP3171 and P{lacW}CSN5L4032
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Pak3 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	First Priority for Reannotation: check LD01046 (5' exon; first intron?). Notice high-scoring protein homologies.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Pak3-RA FBtr0083312  NM_142288   3251   569 Pak3-RC FBtr0305003     4990   569
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Pak3-PA   FBpp0082763   64.1   569   6.00     NP_650545   AAF55316 Pak3-PC   FBpp0293540   64.1   569   6.00
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • PAK-box/P21-Rho-binding (IPR000095) Protein kinase, catalytic domain (IPR000719) Serine/threonine-protein kinase, catalytic domain (IPR002290) Serine/threonine-protein kinase, active site (IPR008271) Protein kinase-like domain (IPR011009) Protein kinase, ATP binding site (IPR017441) Serine/threonine-protein kinase-like domain (IPR017442)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA264049   AA438500   AA735111   AE014297 AAF55316   AI292571   AI389798   AI541866   AI543291   AI546679   AW941812   AX094161   AY060714 AAL28262   AY070845   BG635421   BI162301   BI240856   BP554800   BP554801   BT001523 AAN71278   BT003278 AAO25035   CA807609   CO184086   CO190187   CO264986   CO272515   CO278180   CO279280   CO280749   CO281741   CO283013   CO283560   CO294684   CO295848   CO296340   CO297254   CO299201   CO302813   CO307817   CO309900   CO310433   CO315163   CO320859   CO321753   CO321788   CO324630   CO325722   CO328123   CO333850   CO336486   CO339373   CO341326   CZ472838   DU708601   EC051972   EC052521   EC053024   EC053521   EC055013   EC056440   EC061897   EC062868   EC078424   EC082663   EC085246   EC087536   EC090132   EC210006   EC210157   EC211699   EC213044   EC213338   EC214137   EC214528   EC220073   EC221664   EC223947   EC224565   EC226281   EC226489   EC228356   EC229921   EC234378   EC235294   EC235855   EC237533   EC238950   EC243738   EC246395   EC247775   EC248360   EC250728   EC251840   EC251896   EC257145   EC257812   EC259923   EC262344   EC263264   GH943139   GH958579   GH959538   GH961725   GH964157   GH968298   GH971848   GH972480   GH979746   GH984648
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q8IGZ0 Q8INB3 Q95SL7 Q9VEV1
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0044826    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0044826 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0044826 expression level Developmental Stage   Expression Level embryo 00-02hr    3245 embryo 02-04hr    4263 embryo 04-06hr    5191 embryo 06-08hr    3804 embryo 08-10hr    3626 embryo 10-12hr    3270 embryo 12-14hr    3405 embryo 14-16hr    1909 embryo 16-18hr    1723 embryo 18-20hr    1900 embryo 20-22hr    1687 embryo 22-24hr    1755 larva L1    1042 larva L2    1160 larva L3 12hr old    847 larva L3 puffstage 1-2    722 larva L3 puffstage 3-6    1444 larva L3 puffstage 7-9    1881 white prepupae new    2939 white prepupae 12hr    2874 white prepupae 24hr    2656 pupae 2d postWPP    1754 pupae 3d postWPP    1010 pupae 4d postWPP    926 adult male 01day    1030 adult male 05day    1040 adult male 30day    855 adult female 01day    1653 adult female 05day    2583 adult female 30day    2748 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (3245) embryo 02-04hr  (4263) embryo 04-06hr  (5191) embryo 06-08hr  (3804) embryo 08-10hr  (3626) embryo 10-12hr  (3270) embryo 12-14hr  (3405) embryo 14-16hr  (1909) embryo 16-18hr  (1723) embryo 18-20hr  (1900) embryo 20-22hr  (1687) embryo 22-24hr  (1755) larva L1  (1042) larva L2  (1160) larva L3 12hr old  (847) larva L3 puffstage 1-2  (722) larva L3 puffstage 3-6  (1444) larva L3 puffstage 7-9  (1881) white prepupae new  (2939) white prepupae 12hr  (2874) white prepupae 24hr  (2656) pupae 2d postWPP  (1754) pupae 3d postWPP  (1010) pupae 4d postWPP  (926) adult male 01day  (1030) adult male 05day  (1040) adult male 30day  (855) adult female 01day  (1653) adult female 05day  (2583) adult female 30day  (2748) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (3245) embryo 02-04hr  (4263) embryo 04-06hr  (5191) embryo 06-08hr  (3804) embryo 08-10hr  (3626) embryo 10-12hr  (3270) embryo 12-14hr  (3405) embryo 14-16hr  (1909) embryo 16-18hr  (1723) embryo 18-20hr  (1900) embryo 20-22hr  (1687) embryo 22-24hr  (1755) larva L1    1042 larva L2    1160 larva L3 12hr old    847 larva L3 puffstage 1-2    722 larva L3 puffstage 3-6  1444 larva L3 puffstage 7-9  (1881) white prepupae new  (2939) white prepupae 12hr  (2874) white prepupae 24hr  (2656) pupae 2d postWPP  (1754) pupae 3d postWPP    1010 pupae 4d postWPP    926 adult male 01day    1030 adult male 05day    1040 adult male 30day    855 adult female 01day  (1653) adult female 05day  (2583) adult female 30day  (2748) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    3245 embryo 02-04hr    4263 embryo 04-06hr    5191 embryo 06-08hr    3804 embryo 08-10hr    3626 embryo 10-12hr    3270 embryo 12-14hr    3405 embryo 14-16hr    1909 embryo 16-18hr    1723 embryo 18-20hr    1900 embryo 20-22hr    1687 embryo 22-24hr    1755 larva L1    1042 larva L2    1160 larva L3 12hr old    847 larva L3 puffstage 1-2    722 larva L3 puffstage 3-6    1444 larva L3 puffstage 7-9    1881 white prepupae new    2939 white prepupae 12hr    2874 white prepupae 24hr    2656 pupae 2d postWPP    1754 pupae 3d postWPP    1010 pupae 4d postWPP    926 adult male 01day    1030 adult male 05day    1040 adult male 30day    855 adult female 01day    1653 adult female 05day    2583 adult female 30day    2748 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    3245 embryo 02-04hr    4263 embryo 04-06hr    5191 embryo 06-08hr    3804 embryo 08-10hr    3626 embryo 10-12hr    3270 embryo 12-14hr    3405 embryo 14-16hr    1909 embryo 16-18hr    1723 embryo 18-20hr    1900 embryo 20-22hr    1687 embryo 22-24hr    1755 larva L1    1042 larva L2    1160 larva L3 12hr old    847 larva L3 puffstage 1-2    722 larva L3 puffstage 3-6    1444 larva L3 puffstage 7-9    1881 white prepupae new    2939 white prepupae 12hr    2874 white prepupae 24hr    2656 pupae 2d postWPP    1754 pupae 3d postWPP    1010 pupae 4d postWPP    926 adult male 01day    1030 adult male 05day    1040 adult male 30day    855 adult female 01day    1653 adult female 05day    2583 adult female 30day    2748 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0044826 expression level Developmental Stage   Expression Level embryo 00-02hr    3245 embryo 02-04hr    4263 embryo 04-06hr    5191 embryo 06-08hr    3804 embryo 08-10hr    3626 embryo 10-12hr    3270 embryo 12-14hr    3405 embryo 14-16hr    1909 embryo 16-18hr    1723 embryo 18-20hr    1900 embryo 20-22hr    1687 embryo 22-24hr    1755 larva L1    1042 larva L2    1160 larva L3 12hr old    847 larva L3 puffstage 1-2    722 larva L3 puffstage 3-6    1444 larva L3 puffstage 7-9    1881 white prepupae new    2939 white prepupae 12hr    2874 white prepupae 24hr    2656 pupae 2d postWPP    1754 pupae 3d postWPP    1010 pupae 4d postWPP    926 adult male 01day    1030 adult male 05day    1040 adult male 30day    855 adult female 01day    1653 adult female 05day    2583 adult female 30day    2748 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (3245) embryo 02-04hr  (4263) embryo 04-06hr  (5191) embryo 06-08hr  (3804) embryo 08-10hr  (3626) embryo 10-12hr  (3270) embryo 12-14hr  (3405) embryo 14-16hr  (1909) embryo 16-18hr  (1723) embryo 18-20hr  (1900) embryo 20-22hr  (1687) embryo 22-24hr  (1755) larva L1  (1042) larva L2  (1160) larva L3 12hr old  (847) larva L3 puffstage 1-2  (722) larva L3 puffstage 3-6  (1444) larva L3 puffstage 7-9  (1881) white prepupae new  (2939) white prepupae 12hr  (2874) white prepupae 24hr  (2656) pupae 2d postWPP  (1754) pupae 3d postWPP  (1010) pupae 4d postWPP  (926) adult male 01day  (1030) adult male 05day  (1040) adult male 30day  (855) adult female 01day  (1653) adult female 05day  (2583) adult female 30day  (2748) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (3245) embryo 02-04hr  (4263) embryo 04-06hr  (5191) embryo 06-08hr  (3804) embryo 08-10hr  (3626) embryo 10-12hr  (3270) embryo 12-14hr  (3405) embryo 14-16hr  1909 embryo 16-18hr  1723 embryo 18-20hr  1900 embryo 20-22hr  1687 embryo 22-24hr  1755 larva L1    1042 larva L2    1160 larva L3 12hr old    847 larva L3 puffstage 1-2    722 larva L3 puffstage 3-6  1444 larva L3 puffstage 7-9  1881 white prepupae new  (2939) white prepupae 12hr  (2874) white prepupae 24hr  (2656) pupae 2d postWPP  1754 pupae 3d postWPP    1010 pupae 4d postWPP    926 adult male 01day    1030 adult male 05day    1040 adult male 30day    855 adult female 01day  1653 adult female 05day  (2583) adult female 30day  (2748) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    3245 embryo 02-04hr    4263 embryo 04-06hr    5191 embryo 06-08hr    3804 embryo 08-10hr    3626 embryo 10-12hr    3270 embryo 12-14hr    3405 embryo 14-16hr    1909 embryo 16-18hr    1723 embryo 18-20hr    1900 embryo 20-22hr    1687 embryo 22-24hr    1755 larva L1    1042 larva L2    1160 larva L3 12hr old    847 larva L3 puffstage 1-2    722 larva L3 puffstage 3-6    1444 larva L3 puffstage 7-9    1881 white prepupae new    2939 white prepupae 12hr    2874 white prepupae 24hr    2656 pupae 2d postWPP    1754 pupae 3d postWPP    1010 pupae 4d postWPP    926 adult male 01day    1030 adult male 05day    1040 adult male 30day    855 adult female 01day    1653 adult female 05day    2583 adult female 30day    2748 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    3245 embryo 02-04hr    4263 embryo 04-06hr    5191 embryo 06-08hr    3804 embryo 08-10hr    3626 embryo 10-12hr    3270 embryo 12-14hr    3405 embryo 14-16hr    1909 embryo 16-18hr    1723 embryo 18-20hr    1900 embryo 20-22hr    1687 embryo 22-24hr    1755 larva L1    1042 larva L2    1160 larva L3 12hr old    847 larva L3 puffstage 1-2    722 larva L3 puffstage 3-6    1444 larva L3 puffstage 7-9    1881 white prepupae new    2939 white prepupae 12hr    2874 white prepupae 24hr    2656 pupae 2d postWPP    1754 pupae 3d postWPP    1010 pupae 4d postWPP    926 adult male 01day    1030 adult male 05day    1040 adult male 30day    855 adult female 01day    1653 adult female 05day    2583 adult female 30day    2748 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0044826    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0044826 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: larval/adult midgut. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0044826 expression level Tissue   Expression Level Larval Central Nervous System    279.875 Larval Midgut    665.3 Larval Hindgut    361.3 Larval Malpighian Tubules    395.6 Larval Fat Body    157.3 Larval Salivary Gland    262.3 Larval Trachea    338.75 Larval Carcass    147.45 Adult Head    126.8 Adult Eye    133.15 Adult Brain    243.4 Adult Thoracic-Abdominal Ganglion    226.3 Adult Crop    231.9 Adult Midgut    561.7 Adult Hindgut    267.6 Adult Malpighian Tubules    436.4 Adult Fat Body    139.5 Adult Salivary Gland    159.4 Adult Heart    237.8 Adult VirginFemale Spermatheca    162 Adult InseminatedFemale Spermatheca    177.2 Adult Ovary    312 Adult Testis    105.9 Adult Male Accessory Gland    284.8 Adult Carcass    101.2 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    279.875 Larval Midgut  (665.3) Larval Hindgut    361.3 Larval Malpighian Tubules    395.6 Larval Fat Body    157.3 Larval Salivary Gland    262.3 Larval Trachea    338.75 Larval Carcass    147.45 Adult Head    126.8 Adult Eye    133.15 Adult Brain    243.4 Adult Thoracic-Abdominal Ganglion    226.3 Adult Crop    231.9 Adult Midgut  (561.7) Adult Hindgut    267.6 Adult Malpighian Tubules    436.4 Adult Fat Body    139.5 Adult Salivary Gland    159.4 Adult Heart    237.8 Adult VirginFemale Spermatheca    162 Adult InseminatedFemale Spermatheca    177.2 Adult Ovary    312 Adult Testis    105.9 Adult Male Accessory Gland    284.8 Adult Carcass    101.2 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    279.875 Larval Midgut    665.3 Larval Hindgut    361.3 Larval Malpighian Tubules    395.6 Larval Fat Body    157.3 Larval Salivary Gland    262.3 Larval Trachea    338.75 Larval Carcass    147.45 Adult Head    126.8 Adult Eye    133.15 Adult Brain    243.4 Adult Thoracic-Abdominal Ganglion    226.3 Adult Crop    231.9 Adult Midgut    561.7 Adult Hindgut    267.6 Adult Malpighian Tubules    436.4 Adult Fat Body    139.5 Adult Salivary Gland    159.4 Adult Heart    237.8 Adult VirginFemale Spermatheca    162 Adult InseminatedFemale Spermatheca    177.2 Adult Ovary    312 Adult Testis    105.9 Adult Male Accessory Gland    284.8 Adult Carcass    101.2 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    279.875 Larval Midgut    665.3 Larval Hindgut    361.3 Larval Malpighian Tubules    395.6 Larval Fat Body    157.3 Larval Salivary Gland    262.3 Larval Trachea    338.75 Larval Carcass    147.45 Adult Head    126.8 Adult Eye    133.15 Adult Brain    243.4 Adult Thoracic-Abdominal Ganglion    226.3 Adult Crop    231.9 Adult Midgut    561.7 Adult Hindgut    267.6 Adult Malpighian Tubules    436.4 Adult Fat Body    139.5 Adult Salivary Gland    159.4 Adult Heart    237.8 Adult VirginFemale Spermatheca    162 Adult InseminatedFemale Spermatheca    177.2 Adult Ovary    312 Adult Testis    105.9 Adult Male Accessory Gland    284.8 Adult Carcass    101.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0044826 expression level Tissue   Expression Level Larval Central Nervous System    279.875 Larval Midgut    665.3 Larval Hindgut    361.3 Larval Malpighian Tubules    395.6 Larval Fat Body    157.3 Larval Salivary Gland    262.3 Larval Trachea    338.75 Larval Carcass    147.45 Adult Head    126.8 Adult Eye    133.15 Adult Brain    243.4 Adult Thoracic-Abdominal Ganglion    226.3 Adult Crop    231.9 Adult Midgut    561.7 Adult Hindgut    267.6 Adult Malpighian Tubules    436.4 Adult Fat Body    139.5 Adult Salivary Gland    159.4 Adult Heart    237.8 Adult VirginFemale Spermatheca    162 Adult InseminatedFemale Spermatheca    177.2 Adult Ovary    312 Adult Testis    105.9 Adult Male Accessory Gland    284.8 Adult Carcass    101.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    279.875 Larval Midgut  665.3 Larval Hindgut    361.3 Larval Malpighian Tubules    395.6 Larval Fat Body    157.3 Larval Salivary Gland    262.3 Larval Trachea    338.75 Larval Carcass    147.45 Adult Head    126.8 Adult Eye    133.15 Adult Brain    243.4 Adult Thoracic-Abdominal Ganglion    226.3 Adult Crop    231.9 Adult Midgut  561.7 Adult Hindgut    267.6 Adult Malpighian Tubules    436.4 Adult Fat Body    139.5 Adult Salivary Gland    159.4 Adult Heart    237.8 Adult VirginFemale Spermatheca    162 Adult InseminatedFemale Spermatheca    177.2 Adult Ovary    312 Adult Testis    105.9 Adult Male Accessory Gland    284.8 Adult Carcass    101.2 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    279.875 Larval Midgut    665.3 Larval Hindgut    361.3 Larval Malpighian Tubules    395.6 Larval Fat Body    157.3 Larval Salivary Gland    262.3 Larval Trachea    338.75 Larval Carcass    147.45 Adult Head    126.8 Adult Eye    133.15 Adult Brain    243.4 Adult Thoracic-Abdominal Ganglion    226.3 Adult Crop    231.9 Adult Midgut    561.7 Adult Hindgut    267.6 Adult Malpighian Tubules    436.4 Adult Fat Body    139.5 Adult Salivary Gland    159.4 Adult Heart    237.8 Adult VirginFemale Spermatheca    162 Adult InseminatedFemale Spermatheca    177.2 Adult Ovary    312 Adult Testis    105.9 Adult Male Accessory Gland    284.8 Adult Carcass    101.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    279.875 Larval Midgut    665.3 Larval Hindgut    361.3 Larval Malpighian Tubules    395.6 Larval Fat Body    157.3 Larval Salivary Gland    262.3 Larval Trachea    338.75 Larval Carcass    147.45 Adult Head    126.8 Adult Eye    133.15 Adult Brain    243.4 Adult Thoracic-Abdominal Ganglion    226.3 Adult Crop    231.9 Adult Midgut    561.7 Adult Hindgut    267.6 Adult Malpighian Tubules    436.4 Adult Fat Body    139.5 Adult Salivary Gland    159.4 Adult Heart    237.8 Adult VirginFemale Spermatheca    162 Adult InseminatedFemale Spermatheca    177.2 Adult Ovary    312 Adult Testis    105.9 Adult Male Accessory Gland    284.8 Adult Carcass    101.2 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    126.8   NA  Eye    133.15   NA  Brain    243.4   279.875  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    226.3   NA  Crop    231.9   665.3  Midgut    561.7   361.3  Hindgut    267.6   395.6  Malpighian Tubules    436.4   157.3  Fat Body    139.5   262.3  Salivary Gland    159.4   NA  Heart    237.8   338.75  Trachea    NA   NA  VirginFemale Spermatheca    162   NA  InseminatedFemale Spermatheca    177.2   NA  Ovary    312   NA  Testis    105.9   NA  Male Accessory Gland    284.8   147.45  Carcass    101.2 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_30
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0044826 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG14895-RB
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | recessive Pak3LA00012 semi-lethal | adult stage, with Scer\GAL4pnr-MD237 Pak3GD8481 viable Pak3CB04117 Other Phenotypes Allele visible, with Scer\GAL4pnr-MD237 Pak3GD8481 Phenotype manifest in Allele embryonic/larval cuticle Pak376A Pak327A trichogen cell, with Scer\GAL4pnr-MD237 Pak3GD8481
Classical Alleles ( 12 )
For All Classical Alleles Show
Allele of Pak3 Class Mutagen Stocks Known lesion Pak3LA00012 P-element activity 2 -- Pak3CB-0469-3 1 -- Pak3CB-5374-3 1 -- Pak3CB-6089-3 1 -- Pak3d02472 P-element activity 1 -- Pak3GS3243 1 -- Pak3KG00430 1 -- Pak3NP4472 1 -- Pak327A P-element activity 0 Yes Pak376A P-element activity 0 Yes Pak3CB04117 P-element activity 0 Yes Pak3EP1191 P-element activity 0 --
Alleles Carried on Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Pak3 Class Mutagen Stocks Known lesion Pak3GD8481 in vitro construct - RNAi in vitro construct - regulatory fusion 3 Yes Pak3GL00287 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Pak3KK111386 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Pak3dsRNA.cCa in vitro construct - RNAi 0 Yes Pak3Scer\UAS.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)Exel6269 (Ryder, 2004.4.26) Df(3R)Exel7330 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD8481} NA P{KK111386} NA P{TRiP.GL00287} NA P{UAS-Pak3.GFP} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}Pak3EP1191 NA P{GSV1}CG10405GS3243 NA P{GSV1}EP-167EP-167 NA P{Mae-UAS.6.11}Pak3LA00012 NA P{XP}Pak3d02472 NA miscellaneous insertions P{PTT-GB}Pak3CB04117 NA P{RS3}Pak3CB-0469-3 NA P{RS3}Pak3CB-5374-3 NA P{RS3}Pak3CB-6089-3 NA P{SUPor-P}Pak3KG00430 NA insertion of enhancer trap binary system P{GawB}Pak3NP4472 No
Gene Ontology: Function, Process & Cellular Component ( 5 unique terms )
Terms Based on Experimental Evidence ( 1 term )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
actin filament organization inferred from mutant phenotype (Kiger et al., 2003)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002290, InterPro:IPR017441 (FlyBase Curators et al., 2004-) protein serine/threonine kinase activity inferred from electronic annotation with InterPro:IPR008271 (FlyBase Curators et al., 2004-) receptor signaling protein serine/threonine kinase activity non-traceable author statement (Morrison et al., 2000)
Biological Process
CV term Evidence References
protein phosphorylation inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002290, InterPro:IPR008271 (FlyBase Curators et al., 2004-) non-traceable author statement (Morrison et al., 2000)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Pak3 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 67040 DroID - A comprehensive database of gene and protein interactions. • FBgn0044826 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41995
Orthologs
Genome-wide drosophilid orthologs	Dana\GF16514 Dere\GG19949 Dgri\GH18242 Dmoj\GI10198 Dper\GL22239 Dpse\Pak3 Dsec\GM15431 Dsim\GD20287 Dvir\GJ23486 Dwil\GK14065 Dyak\GE26311
Curated drosophilid orthologs	Dpse\Pak3 (Mentzel and Raabe, 2005, )
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL000489-PA Aedes aegypti (mosquito) AGAP001790-PA Anopheles gambiae (mosquito) At1g53570.1 Arabidopsis thaliana (wall cress) BGIBMGA007577-PA Bombyx mori (silk worm) CN20110 Caenorhabditis brenneri (small nematode) CPIJ006042 Culex pipens (mosquito) RP40240 Caenorhabditis remanei (nematode) GL50803_15514 Giardia lamblia (flagellated protozoan) KLLA0B13607g Kluyveromyces lactis (yeast) LmjF17.0490 Leishmania major (virus) 36217 Monocosta brevicollis (choanoflagellate) XP_001601853.1 Nasonia vitripennis (wasp) PHUM002418-PA Pediculus humanus (human body louse) 123192 Physcomitrella patens (moss) 147244 Physcomitrella patens (moss) 178475 Physcomitrella patens (moss) YHL007C Saccharomyces cerevisiae (budding yeast) XP_966936.2 Tribolium castaneum (red flour beetle) Tc00.1047053503473.10 Trypanosoma cruzi (parasitic trypanosome) Tc00.1047053506743.160 Trypanosoma cruzi (parasitic trypanosome) 83472.m00456 Trichomonas vaginalis (parasitic flagellated protozoan) 95629.m00070 Trichomonas vaginalis (parasitic flagellated protozoan) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV18586 NV15829 LH17234 ISCW000704 FBgn0243341 FBgn0228088 FBgn0216071 FBgn0214186 FBgn0211679 FBgn0210584 FBgn0191762 FBgn0191358 FBgn0170349 FBgn0165836 JGI_V11_301193 JGI_V11_301285 FBgn0073369 FBgn0070283 FBgn0159831 FBgn0159334 FBgn0145735 FBgn0132964 FBgn0014001 FBgn0126996 FBgn0125710 FBgn0112149 FBgn0105651 FBgn0095374 FBgn0093535 CPIJ006042 BGIBMGA010956 BGIBMGA007577 ACYPI001405 GB18379 GB13173 AGAP002986 AGAP001790 AAEL002594 AAEL000489 PB21029 PHUM310720 PHUM373950 GLEAN_10914 GLEAN_02075
Stocks & Reagents
Stocks Listed in FlyBase ( 14 )
Bloomington	13661 y1; ry506 P{SUPor-P}Pak3KG00430 35373 y1 sc* v1; P{TRiP.GL00287}attP2 22211 y1 w*; P{Mae-UAS.6.11}Pak3LA00012
Harvard	- P{XP}Pak3d02472
Kyoto	123187 w1118; P{RS3}Pak3CB-0469-3 123594 w1118; P{RS3}Pak3CB-5374-3 123909 w1118; P{RS3}Pak3CB-6089-3 120012 y* w*; P{Mae-UAS.6.11}Pak3LA00012
VDRC	v107260 P{KK111386}VIE-260B v39844 w1118; P{GD8481}v39844 v44607 w1118; P{GD8481}v44607 v39843 w1118; P{GD8481}v39843/TM3
	More stocks available...
Genomic Clones ( 1 )
	BACR01H23 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 98 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH15507 GH26692 LD10376 RE01659
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH21061 LD01046 LD07473 RE37791 SD07816 SD10005 SD10007 SD12829
Other clones	8663 17407 20204 31576 41445 51277 53729 59709 59747 61658 66752 69845 75294 78003 80371 82841 97295 99139 104588 108652 119604 122886 145769 149552 174211 183522 191424 214257.33 214443.35 214503.35 214563.35 214575.20 282353 557903 557905 CK01.58B.G1 CK01.125D.H5 CK01.126B.F6 EC27082 EC051972 EC052521 EC053024 EC053521 EC055013 EC056440 EK046925 EK107451 EK129216 EK133943 EK141135 EK154757 EK158705 EK162017 EK163649 EK174754 EK184939 EK186319 EK186363 EK194415 EK197324 EK207443 EK211746 EK213422 EK216463 EK219723 EK221633 EK239544 EK255760 EK288526 EK308153 EN12630 EP01761 EP08617 EP12251 ESG018c.E21.1_G11 FGM231G09 RP004029639 RP004196039 RP004247909 RP004253341 RP004396607 RP004406558 RP004456721 RP004506523 RP004532212 RP004556866
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0044826 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41995
Antibody Information
	polyclonal (Bahri et al., 2010)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: Pak3 anon-WO0118547.285 (FlyBase, 1996-) Source for merge of: Pak3 CG14895 (Burbelo, 2001.7.27)
Additional comments	Source for merge of Pak3 anon-WO0118547.285 was sequence comparison (date:051113). (FlyBase, 1996-)
Other Comments
	RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 and S2R+ cells: increased or polarized (uneven) accumulation of F-actin. (Kiger et al., 2003) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) Area matching Drosophila EST AA438500 (inverted). (Davies, 2001.3.15)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_142288 RefSeq (Proteins) • NP_650545 DDBJ / EMBL / GenBank • AA438500 AAF55316 AAL28262 AAN13710 AAN71278 AAO25035 AI292571 AW941812 AX094161 AY060714 BI162301 BT001523 BT003278 CZ472838 DU708601 Entrez Gene - A searchable database of RefSeq genes. • 41995 UniProtKB/TrEMBL • Q8IGZ0 Q8INB3 Q9VEV1 Q95SL7
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • PAK-box/P21-Rho-binding (IPR000095) Protein kinase, catalytic domain (IPR000719) Serine/threonine-protein kinase, catalytic domain (IPR002290) Serine/threonine-protein kinase, active site (IPR008271) Protein kinase-like domain (IPR011009) Protein kinase, ATP binding site (IPR017441) Serine/threonine-protein kinase-like domain (IPR017442)
Linkouts
	BioGRID - A database of protein and genetic interactions • 67040 DroID - A comprehensive database of gene and protein interactions. • FBgn0044826 DRSC - Results from RNAi screens. • FBgn0044826 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0044826 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG14895-RB FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0044826 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41995 InParanoid A subset of ortholog calls from InParanoid. • AAEL000489-PA Aedes aegypti (mosquito) AGAP001790-PA Anopheles gambiae (mosquito) At1g53570.1 Arabidopsis thaliana (wall cress) BGIBMGA007577-PA Bombyx mori (silk worm) CN20110 Caenorhabditis brenneri (small nematode) CPIJ006042 Culex pipens (mosquito) RP40240 Caenorhabditis remanei (nematode) GL50803_15514 Giardia lamblia (flagellated protozoan) KLLA0B13607g Kluyveromyces lactis (yeast) LmjF17.0490 Leishmania major (virus) 36217 Monocosta brevicollis (choanoflagellate) XP_001601853.1 Nasonia vitripennis (wasp) PHUM002418-PA Pediculus humanus (human body louse) 123192 Physcomitrella patens (moss) 147244 Physcomitrella patens (moss) 178475 Physcomitrella patens (moss) YHL007C Saccharomyces cerevisiae (budding yeast) XP_966936.2 Tribolium castaneum (red flour beetle) Tc00.1047053503473.10 Trypanosoma cruzi (parasitic trypanosome) Tc00.1047053506743.160 Trypanosoma cruzi (parasitic trypanosome) 83472.m00456 Trichomonas vaginalis (parasitic flagellated protozoan) 95629.m00070 Trichomonas vaginalis (parasitic flagellated protozoan) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41995 modMine - Data generated by the modENCODE project. • FBgn0044826 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV18586 NV15829 LH17234 ISCW000704 FBgn0243341 FBgn0228088 FBgn0216071 FBgn0214186 FBgn0211679 FBgn0210584 FBgn0191762 FBgn0191358 FBgn0170349 FBgn0165836 JGI_V11_301193 JGI_V11_301285 FBgn0073369 FBgn0070283 FBgn0159831 FBgn0159334 FBgn0145735 FBgn0132964 FBgn0014001 FBgn0126996 FBgn0125710 FBgn0112149 FBgn0105651 FBgn0095374 FBgn0093535 CPIJ006042 BGIBMGA010956 BGIBMGA007577 ACYPI001405 GB18379 GB13173 AGAP002986 AGAP001790 AAEL002594 AAEL000489 PB21029 PHUM310720 PHUM373950 GLEAN_10914 GLEAN_02075
Synonyms & Secondary IDs ( 11 )
Reported As
Symbol Synonym	anon-WO0118547.285   CG14895 (Dan et al., 2001, Laviolette et al., 2005, Molnar et al., 2006, Keleman et al., 2009.8.5, Ni et al., 2011.7.19) DmPAK3 (Mentzel and Raabe, 2005, Mentzel et al., 2009) D-Pak3 (Mentzel et al., 2003) DPAK3 (Chen et al., 2004) dPAK3 (Burbelo, 2001.7.27, Pirone et al., 2001) DPak3 (Hofmann et al., 2004) PaK3 (Bjorklund et al., 2006) pak3 (Orian et al., 2003, Davidson et al., 2009, Bahri et al., 2010) PAK3 (Morrison et al., 2000) Pak3 (Laviolette et al., 2005, Buszczak et al., 2007, Molnar et al., 2006, Asano et al., 2009)
Name Synonym	Pak3
Secondary FlyBase IDs
	FBgn0038430 FBgn0062119
References ( 41 )
Generate a list of	All references relating to this gene ( 41 )
List References by type	Research paper  ( 15 ) Supplementary material  ( 5 ) Review  ( 3 ) Personal communication to FlyBase  ( 7 ) Abstract  ( 1 ) FlyBase analysis  ( 3 ) Patent  ( 1 ) DNA/RNA sequence record  ( 1 ) Computer file  ( 3 ) Automatic genome annotation  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 1 )
	Bahri et al., 2010, Development 137(12): 2023--2032 The leading edge during dorsal closure as a model for epithelial plasticity: Pak is required for recruitment of the Scribble complex and septate junction formation. [FBrf0210918] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...