FB2021_01, released Feb 18, 2021

Gene: Dmel\Pak3

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General Information
Symbol
Dmel\Pak3
Species
D. melanogaster
Name
Pak3
Annotation Symbol
CG14895
Feature Type
protein_coding_gene
FlyBase ID
FBgn0044826
Gene Model Status
Current
Stock Availability
24 publicly available
Gene Snapshot
Pak3 (Pak3) encodes a Ser/Thr kinase implicated in myoblast fusion, synapse development, wound healing and border cell migration. It functions downstream of the small Rac GTPases to regulate actin cytoskeletal organization. [Date last reviewed: 2019-03-14]
Other Summaries
Also Known As

DmPAK3, dPAK3

Key Links
Genomic Location
Cytogenetic map
89B16-89B17
Sequence location
3R:16,445,007..16,453,821 [-]
Recombination map

3-58

RefSeq locus
NT_033777 REGION:16445007..16453821
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (14 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
enables protein homodimerization activity
inferred from physical interaction with FLYBASE:Pak3; FB:FBgn0044826
(Mentzel and Raabe, 2005)
enables protein serine/threonine kinase activity
inferred from direct assay
(Mentzel and Raabe, 2005)
enables small GTPase binding
inferred from physical interaction with FLYBASE:Rac1; FB:FBgn0010333
(Mentzel and Raabe, 2005)
inferred from physical interaction with FLYBASE:Rac2; FB:FBgn0014011
(Mentzel and Raabe, 2005)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
enables ATP binding
inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002290, InterPro:IPR017441
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
enables protein serine/threonine kinase activity
inferred from biological aspect of ancestor with PANTHER:PTN000684825
(assigned by GO_Central )
(Gaudet et al., 2011)
Biological Process (8 terms)
Terms Based on Experimental Evidence (5 terms)
CV Term
Evidence
References
involved_in actin filament organization
inferred from genetic interaction with FLYBASE:Rac1; FB:FBgn0010333
(Mentzel and Raabe, 2005)
involved_in myoblast fusion
inferred from mutant phenotype
(Duan et al., 2012)
involved_in positive regulation of synapse assembly
inferred from genetic interaction with FLYBASE:spas; FB:FBgn0039141
(Ozdowski et al., 2011)
involved_in protein autophosphorylation
inferred from direct assay
(Mentzel and Raabe, 2005)
involved_in protein phosphorylation
inferred from direct assay
(Mentzel and Raabe, 2005)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
involved_in activation of protein kinase activity
inferred from biological aspect of ancestor with PANTHER:PTN000684825
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in regulation of axonogenesis
inferred from biological aspect of ancestor with PANTHER:PTN000685295
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in stress-activated protein kinase signaling cascade
inferred from biological aspect of ancestor with PANTHER:PTN000684825
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
located_in cytoplasm
inferred from direct assay
(Mentzel and Raabe, 2005)
located_in ruffle
inferred from direct assay
(Mentzel and Raabe, 2005)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
located_in cytoplasm
inferred from biological aspect of ancestor with PANTHER:PTN000684825
(assigned by GO_Central )
(Gaudet et al., 2011)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
P21-ACTIVATED KINASES -
PAK proteins are serine/threonine kinases characterised by an C-terminal STE20 kinase domain and an N-terminal p21-binding domain which binds RhoGTPases. PAKs are autoinhibited by homodimerization. PAKs are involved in the regulation of cytoskeleton organisation and other signaling cascades. (Adapted from FBrf0187582).
Gene Model and Products
Number of Transcripts
3
Number of Unique Polypeptides
2

Please see the JBrowse view of Dmel\Pak3 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Stop-codon suppression (UAG) postulated; FBrf0216884.

Gene model reviewed during 5.44

Gene model reviewed during 5.48

Sequence Ontology: Class of Gene
gene_with_stop_codon_read_through
(Jungreis et al., 2011)
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Pak3-RA
FBtr0083312
NM_142288
3244
569
Pak3-RC
FBtr0305003
NM_169719
4983
569
Pak3-RD
FBtr0330047
NM_001275709
4983
583
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
Pak3-PA
FBpp0082763
64.1
569
6.00
NP_650545
AAF55316
Pak3-PC
FBpp0293540
64.1
569
6.00
NP_732141
AAN13710
Pak3-PD
FBpp0303080
65.7
583
6.34
NP_001262638
AGB96018
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

569 aa isoforms: Pak3-PA, Pak3-PC
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Pak3 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 14
fusion competent cell
(Duan et al., 2012)
embryonic stage 13
embryonic dorsal epidermis
(Bahri et al., 2010)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in cytoplasm
inferred from direct assay
(Mentzel and Raabe, 2005)
located_in ruffle
inferred from direct assay
(Mentzel and Raabe, 2005)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Pak3 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 42 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 15 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Pak3
Transgenic constructs containing regulatory region of Pak3
Aberrations (Deficiencies and Duplications) ( 3 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal | recessive
lethal - all die before end of first instar larval stage | recessive
lethal - all die before end of larval stage (with Pak3d02472)
lethal - all die before end of larval stage (with Pak3Df)
partially lethal - majority die
partially lethal - majority die (with Pak3Df)
partially lethal - majority die (with Pak3e00329)
partially lethal - majority die | adult stage, with Scer\GAL4pnr-MD237
partially lethal - majority live
partially lethal - majority live (with Pak3d02472)
partially lethal - majority live (with Pak3e00329)
viable
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
Sterility
Allele
female semi-sterile, with Scer\GAL4nos.PG, Scer\GAL4VP16.nos.UTR, Scer\GAL4VP16.otu
Other Phenotypes
Allele
abnormal neuroanatomy, with Scer\GAL4221
abnormal neuroanatomy, with Scer\GAL4elav.PU
abnormal neuroanatomy, with Scer\GAL4ppk.PG
abnormal neurophysiology (with Pak3d02472)
abnormal neurophysiology (with Pak3e00329)
flightless, with Scer\GAL4Mef2.PR
visible, with Scer\GAL4Mef2.PR
visible, with Scer\GAL4pnr-MD237
Phenotype manifest in
Allele
abdominal dorsal multidendritic neuron ddaC | third instar larval stage, with Scer\GAL4ppk.PG
abdominal dorsal multidendritic neuron ddaD | third instar larval stage, with Scer\GAL4221
abdominal dorsal multidendritic neuron ddaE | third instar larval stage, with Scer\GAL4221
dendritic tree | third instar larval stage, with Scer\GAL4221
dendritic tree | third instar larval stage, with Scer\GAL4ppk.PG
embryonic/larval cuticle
embryonic/larval neuromuscular junction, with Scer\GAL4elav.PU
embryonic myoblast
embryonic myoblast, with Pak3del, Scer\GAL4twi.PG
embryonic myoblast, with Pak3K322A.UAS.C.Tag:V5, Scer\GAL4twi.PG
embryonic myoblast, with Scer\GAL4twi.PG
filamentous actin
microtubule, with Scer\GAL4elav.PU
NMJ bouton, with Scer\GAL4elav.PU
trichogen cell, with Scer\GAL4pnr-MD237
wing, with Scer\GAL4Mef2.PR
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (7)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\PAK1
5 of 15
Yes
No
Hsap\PAK2
5 of 15
Yes
No
Hsap\PAK3
5 of 15
Yes
No
Hsap\PAK4
3 of 15
No
No
Hsap\PAK5
3 of 15
No
No
Hsap\PAK6
3 of 15
No
No
Hsap\BUB1B-PAK6
1 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (6)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Pak3
6 of 15
Yes
No
Mmus\Pak1
5 of 15
No
No
Mmus\Pak2
5 of 15
No
No
Mmus\Pak6
3 of 15
No
No
Mmus\Pak4
2 of 15
No
No
Mmus\Pak7
2 of 15
No
No
Rattus norvegicus (Norway rat) (7)
Rnor\Pak1
5 of 13
Yes
No
Rnor\Pak3
5 of 13
Yes
No
Rnor\LOC100910732
4 of 13
No
No
Rnor\Pak2
3 of 13
No
No
Rnor\Pak4
3 of 13
No
No
Rnor\Pak6
3 of 13
No
No
Rnor\Pak7
3 of 13
No
No
Xenopus tropicalis (Western clawed frog) (6)
Xtro\pak2
4 of 12
Yes
No
Xtro\pak1
3 of 12
No
No
Xtro\pak3
3 of 12
No
No
Xtro\pak4
3 of 12
No
No
Xtro\pak6
3 of 12
No
No
Xtro\pak5
2 of 12
No
No
Danio rerio (Zebrafish) (7)
Drer\pak1
5 of 15
Yes
No
Drer\pak2a
5 of 15
Yes
No
Drer\LOC100536197
4 of 15
No
Yes
Drer\pak2b
4 of 15
No
No
Drer\pak4
3 of 15
No
No
Drer\pak6b
3 of 15
No
No
Drer\pak7
3 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (3)
Cele\max-2
7 of 15
Yes
Yes
Cele\pak-1
5 of 15
No
No
Cele\pak-2
3 of 15
No
No
Arabidopsis thaliana (thale-cress) (5)
Atha\AT3G15220
1 of 9
Yes
No
Atha\ATMAP4K ALPHA1
1 of 9
Yes
No
Atha\MAP3KA
1 of 9
Yes
No
Atha\MAPKKK5
1 of 9
Yes
No
Atha\SIK1
1 of 9
Yes
No
Saccharomyces cerevisiae (Brewer's yeast) (3)
Scer\STE20
8 of 15
Yes
No
Scer\CLA4
6 of 15
No
Yes
Scer\SKM1
6 of 15
No
No
Schizosaccharomyces pombe (Fission yeast) (2)
Spom\shk1
7 of 12
Yes
No
Spom\shk2
5 of 12
No
Yes
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190697 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00010459
Drosophila simulans
Dsim\GD20287
Drosophila sechellia
Dsec\GM15431
Drosophila erecta
Dere\GG19949
Drosophila yakuba
Dyak\GE26311
Drosophila ananassae
Dana\GF16514
Drosophila pseudoobscura pseudoobscura
Dpse\Pak3
Drosophila persimilis
Dper\GL22239
Drosophila willistoni
Dwil\GK14065
Drosophila virilis
Dvir\GJ23486
Drosophila mojavensis
Dmoj\GI10198
Drosophila grimshawi
Dgri\GH18242
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091503FX )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17005455
Musca domestica
House fly
Mdoa\17005448
Musca domestica
House fly
Mdoa\17004434
Musca domestica
House fly
Mdoa\17004421
Musca domestica
House fly
Mdoa\17016159
Musca domestica
House fly
Mdoa\17011977
Glossina morsitans
Tsetse fly
Gmor\GMOY009690
Glossina morsitans
Tsetse fly
Gmor\GMOY001677
Glossina morsitans
Tsetse fly
Gmor\GMOY007691
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_10526
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_03816
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_03761
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_00572
Mayetiola destructor
Hessian fly
Mdes\Mdes005604
Mayetiola destructor
Hessian fly
Mdes\Mdes002302
Mayetiola destructor
Hessian fly
Mdes\Mdes013115
Mayetiola destructor
Hessian fly
Mdes\Mdes009619
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL007931
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL002594
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL000489
Anopheles darlingi
American malaria mosquito
Adar\ADAC004548
Anopheles darlingi
American malaria mosquito
Adar\ADAC008558
Anopheles darlingi
American malaria mosquito
Adar\ADAC004320
Anopheles gambiae
Malaria mosquito
Agam\AGAP002986
Anopheles gambiae
Malaria mosquito
Agam\AGAP010874
Anopheles gambiae
Malaria mosquito
Agam\AGAP001790
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ004448
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ006042
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0548 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA007577
Bombyx mori
Silkmoth
Bmor\BGIBMGA010956
Bombyx mori
Silkmoth
Bmor\BGIBMGA010955
Bombyx mori
Silkmoth
Bmor\BGIBMGA008506
Danaus plexippus
Monarch butterfly
Dple\DPOGS206674-PA
Danaus plexippus
Monarch butterfly
Dple\DPOGS204301-PA
Danaus plexippus
Monarch butterfly
Dple\DPOGS203416-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL003627
Heliconius melpomene
Postman butterfly
Hmel\HMEL015167
Heliconius melpomene
Postman butterfly
Hmel\HMEL012313
Apis florea
Little honeybee
Aflo\3195046868
Apis florea
Little honeybee
Aflo\2189776724
Apis florea
Little honeybee
Aflo\2256741060
Apis mellifera
Western honey bee
Amel\GB48749
Apis mellifera
Western honey bee
Amel\LOC551659
Apis mellifera
Western honey bee
Amel\mbt
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP19291
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP20000
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP22301
Bombus terrestris
Buff-tailed bumblebee
Bter\15211849
Bombus terrestris
Buff-tailed bumblebee
Bter\15212167
Bombus terrestris
Buff-tailed bumblebee
Bter\15213975
Linepithema humile
Argentine ant
Lhum\LH17234
Linepithema humile
Argentine ant
Lhum\LH11301
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:00192e
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG012515
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG037323
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG017820
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG029342
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_06219
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_08178
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_04522
Tribolium castaneum
Red flour beetle
Tcas\TC010914
Tribolium castaneum
Red flour beetle
Tcas\TC002075
Tribolium castaneum
Red flour beetle
Tcas\GLEAN_08012
Pediculus humanus
Human body louse
Phum\PHUM373950
Pediculus humanus
Human body louse
Phum\PHUM392290
Pediculus humanus
Human body louse
Phum\PHUM310720
Rhodnius prolixus
Kissing bug
Rpro\RPRC005491
Rhodnius prolixus
Kissing bug
Rpro\RPRC011211
Cimex lectularius
Bed bug
Clec\CLEC006881
Cimex lectularius
Bed bug
Clec\CLEC009877
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI004180
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI001405
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_01820
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_04560
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0674 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR005367
Strigamia maritima
European centipede
Smar\SMAR014593
Ixodes scapularis
Black-legged tick
Isca\ISCW022207
Ixodes scapularis
Black-legged tick
Isca\ISCW000704
Stegodyphus mimosarum
African social velvet spider
Smim\KFM73493.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM77356.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM63103.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM76380.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM76381.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur02g04160
Tetranychus urticae
Two-spotted spider mite
Turt\tetur11g03920
Tetranychus urticae
Two-spotted spider mite
Turt\tetur02g11510
Tetranychus urticae
Two-spotted spider mite
Turt\tetur02g04150
Tetranychus urticae
Two-spotted spider mite
Turt\tetur13g00010
Tetranychus urticae
Two-spotted spider mite
Turt\tetur13g00020
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_301193
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_306799
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_301285
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_204885
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G04H8 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Pak1
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Pak(p21)ph
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000007043
Gallus gallus
Domestic chicken
Ggal\PAK3
Gallus gallus
Domestic chicken
Ggal\PAK1
Gallus gallus
Domestic chicken
Ggal\PAK2
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (15)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
Pak
6 of 10
mbt
5 of 10
Dsor1
1 of 10
fray
1 of 10
GckIII
1 of 10
hep
1 of 10
hpo
1 of 10
hppy
1 of 10
lic
1 of 10
Mekk1
1 of 10
Mkk4
1 of 10
msn
1 of 10
Slik
1 of 10
Stlk
1 of 10
Tao
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 1 )
    Modifiers Based on Experimental Evidence ( 2 )
    Allele
    Disease
    Interaction
    References
    Pak3d02472
    ameliorates  hereditary spastic paraplegia
    modeled by spas5.75
    (Ozdowski et al., 2011)
    Pak3Df
    ameliorates  hereditary spastic paraplegia
    modeled by spas5.75
    (Ozdowski et al., 2011)
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    PAK1; p21 (RAC1) activated kinase 1
    5 of 15
    602590
      PAK2; p21 (RAC1) activated kinase 2
      5 of 15
      605022
          PAK3; p21 (RAC1) activated kinase 3
          5 of 15
          300142
          PAK4; p21 (RAC1) activated kinase 4
          3 of 15
          605451
              PAK5; p21 (RAC1) activated kinase 5
              3 of 15
              608038
                  PAK6; p21 (RAC1) activated kinase 6
                  3 of 15
                  608110
                      Functional Complementation Data
                      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
                      Interactions
                      Summary of Physical Interactions
                      Summary of Genetic Interactions
                      esyN Network Diagram
                      esyN Network Key:
                      Suppression
                      Enhancement
                      View in Interactions Browser
                      Please look at the allele data for full details of the genetic interactions
                      Starting gene(s)
                      Interaction type
                      Interacting gene(s)
                      Reference
                      Pak3
                      enhanceable
                      Pak
                      (Duan et al., 2012, Bahri et al., 2010)
                      Pak3
                      suppressible
                      Pak
                      (Duan et al., 2012)
                      Pak3
                      enhanceable
                      SCAR
                      (Duan et al., 2012)
                      Pak3
                      enhanceable
                      WASp
                      (Duan et al., 2012)
                      Starting gene(s)
                      Interaction type
                      Interacting gene(s)
                      Reference
                      kn
                      suppressible
                      Pak3
                      (Hattori et al., 2013)
                      Pak
                      enhanceable
                      Pak3
                      (Bahri et al., 2010)
                      SCAR
                      enhanceable
                      Pak3
                      (Duan et al., 2012)
                      spas
                      suppressible
                      Pak3
                      (Ozdowski et al., 2011)
                      spas
                      enhanceable
                      Pak3
                      (Ozdowski et al., 2011)
                      External Data
                      Linkouts
                      BioGRID - A database of protein and genetic interactions.
                      DroID - A comprehensive database of gene and protein interactions.
                      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
                      MIST (genetic) - An integrated Molecular Interaction Database
                      MIST (protein-protein) - An integrated Molecular Interaction Database
                      Pathways
                      Signaling Pathways (FlyBase)
                      Metabolic Pathways
                      External Data
                      Linkouts
                      KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
                      Genomic Location and Detailed Mapping Data
                      Chromosome (arm)
                      3R
                      Recombination map

                      3-58

                      Cytogenetic map
                      89B16-89B17
                      Sequence location
                      3R:16,445,007..16,453,821 [-]
                      FlyBase Computed Cytological Location
                      Cytogenetic map
                      Evidence for location
                      89B16-89B17
                      Limits computationally determined from genome sequence between P{PZ}gish04895&P{EP}EP3171 and P{lacW}CSN5L4032
                      Experimentally Determined Cytological Location
                      Cytogenetic map
                      Notes
                      References
                      Experimentally Determined Recombination Data
                      Location

                      3-58

                      (FlyBase, 2016)

                      3-58.4

                      (Comeron et al., 2012)
                      Left of (cM)
                      Right of (cM)
                      Notes
                      Stocks and Reagents
                      Stocks (24)
                      Genomic Clones (15)
                       

                      Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

                      cDNA Clones (95)
                       

                      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

                      cDNA clones, fully sequenced
                      BDGP DGC clones
                      Other clones
                        Drosophila Genomics Resource Center cDNA clones

                        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

                        cDNA Clones, End Sequenced (ESTs)
                        BDGP DGC clones
                        Other clones
                        RNAi and Array Information
                        Linkouts
                        DRSC - Results frm RNAi screens
                        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
                        Antibody Information
                        Laboratory Generated Antibodies
                         

                        polyclonal

                        (Duan et al., 2012, Bahri et al., 2010)
                        Commercially Available Antibodies
                         
                        Other Information
                        Relationship to Other Genes
                        Source for database identify of
                        Source for database merge of

                        Source for merge of: Pak3 CG14895

                        (Burbelo, 2001.7.27)

                        Source for merge of: Pak3 anon-WO0118547.285

                        (FlyBase, 1996-)
                        Additional comments

                        Source for merge of Pak3 anon-WO0118547.285 was sequence comparison ( date:051113 ).

                        (FlyBase, 1996-)
                        Other Comments

                        dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

                        (Kiger et al., 2003)

                        RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 and S2R+ cells: increased or polarized (uneven) accumulation of F-actin.

                        (Kiger et al., 2003)

                        Area matching Drosophila EST AA438500 (inverted).

                        (Davies, 2001.3.15)
                        Origin and Etymology
                        Discoverer
                        Etymology
                        Identification
                        External Crossreferences and Linkouts ( 56 )
                        Sequence Crossreferences
                        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                        UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                        Other crossreferences
                        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                        Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                        Flygut - An atlas of the Drosophila adult midgut
                        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
                        iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                        InterPro - A database of protein families, domains and functional sites
                        KEGG Genes - Molecular building blocks of life in the genomic space.
                        modMine - A data warehouse for the modENCODE project
                        SignaLink - A signaling pathway resource with multi-layered regulatory networks.
                        Linkouts
                        BioGRID - A database of protein and genetic interactions.
                        DroID - A comprehensive database of gene and protein interactions.
                        DRSC - Results frm RNAi screens
                        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
                        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                        FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                        FlyMine - An integrated database for Drosophila genomics
                        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
                        KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
                        MIST (genetic) - An integrated Molecular Interaction Database
                        MIST (protein-protein) - An integrated Molecular Interaction Database
                        Synonyms and Secondary IDs (15)
                        Datasets (0)
                        Study focus (0)
                        Experimental Role
                        Project
                        Project Type
                        Title
                        References (95)