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Gene Dmel\ham

General Information
Symbol	Dmel\ham	Species	D. melanogaster
Name	hamlet	Annotation symbol	CG31753
Feature type	protein_coding_gene	FlyBase ID	FBgn0045852
Gene Model Status	Current	Stock availability	8 publicly available
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	37A2-37A4	Sequence location	2L:18,762,874..18,787,500 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene hamlet is referred to in FlyBase by the symbol Dmel\ham (CG31753, FBgn0045852). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: sequence-specific DNA binding transcription factor activity. There is experimental evidence that it is involved in the biological process: regulation of dendrite morphogenesis; regulation of transcription, DNA-dependent; sensory organ development; neuron development. 12 alleles are reported. The phenotypes of these alleles are annotated with: trichogen cell; dorsal abdominal cluster; embryonic/larval nervous system; chaeta; multidendritic neuron; abdominal ventral sensillum campaniformium vc4a; tormogen cell; eo neuron. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Zinc finger, C2H2; Zinc finger, C2H2-like; Zinc finger, C2H2-type/integrase, DNA-binding. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of very low expression. Peak expression observed at stages throughout embryogenesis, during early larval stages. Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. Gene sequence location is 2L:18762874..18787500.
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01	Sequence features BKN22290 HFA02512 HFA02513 HFA01657 HFA02070
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 37A2-37A4   Limits computationally determined from genome sequence between P{EP}CG10413EP2164 and P{lacW}l(2)37Dbk16106&P{lacW}Catsupk05424
Experimentally Determined Cytological Location
Cytogenetic map Notes References 37A-37A     (Moore, 2002.10.17)
Experimentally Determined Recombination Data
Location
Left of (cM)	tup (Moore et al., 2002)
Right of (cM)	msl-1 (Moore et al., 2002)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\ham for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene merge based on AY166585.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) ham-RB FBtr0305620     4242   990 ham-RC FBtr0305621     4243   990
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein ham-PB   FBpp0294066   109.2   990   7.35       ham-PC   FBpp0294067   109.2   990   7.35
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Zinc finger, C2H2 (IPR007087) Zinc finger, C2H2-type/integrase, DNA-binding (IPR013087) Zinc finger, C2H2-like (IPR015880)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AY166585 AAN86032   BT132713   CO179145   CO196211   CO196251   CO269392   CO290117   CO306423   CO311694   CZ483172   DU708599   EC058804   EC058976   EC064889   EC076948   EC089570   EC091041   EC201106   EC214668   EC240129   EC240130   EC259215   EL875939
UniProtKB/Swiss-Prot	Q8I7Z8
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 5   organism | 80-90% egg length & ventral   (Moore et al., 2002) embryonic stage 5 -- 15   procephalic neurogenic region   (Moore et al., 2002) embryonic stage 11 -- 15   presumptive embryonic/larval peripheral nervous system   (Moore et al., 2002) external sensory organ precursor cell IIIb   (Moore et al., 2002) embryonic stage 12   dorsal abdominal cluster   (Moore et al., 2002) Comment:weak expression dorsal thoracic cluster   (Moore et al., 2002) Comment:weak expression lateral abdominal cluster   (Moore et al., 2002) Comment:strong expression lateral thoracic cluster   (Moore et al., 2002) Comment:strong expression embryonic stage 13   dorsal abdominal cluster   (Moore et al., 2002) Comment:strong expression dorsal thoracic cluster   (Moore et al., 2002) Comment:strong expression lateral abdominal cluster   (Moore et al., 2002) Comment:weak expression lateral thoracic cluster   (Moore et al., 2002) Comment:weak expression ventral abdominal cluster   (Moore et al., 2002) Comment:strong expression ventral thoracic cluster   (Moore et al., 2002) Comment:strong expression embryonic stage 14 -- 15   thecogen cell of dorsal thoracic cluster   (Moore et al., 2002) thecogen cell of dorsal abdominal cluster   (Moore et al., 2002) embryonic stage 14 -- 17   eo neuron of dorsal thoracic cluster   (Moore et al., 2002) eo neuron of dorsal abdominal cluster   (Moore et al., 2002)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage 5   organism | 80-90% egg length & ventral   (Moore et al., 2002) embryonic stage 5 -- 15   procephalic neurogenic region   • nucleus (Moore et al., 2002) embryonic stage 11 -- 15   presumptive embryonic/larval peripheral nervous system   • nucleus (Moore et al., 2002) external sensory organ precursor cell IIIb   • nucleus (Moore et al., 2002) embryonic stage 12   dorsal abdominal cluster   • nucleus (Moore et al., 2002) Comment:weak expression dorsal thoracic cluster   • nucleus (Moore et al., 2002) Comment:weak expression lateral abdominal cluster   • nucleus (Moore et al., 2002) Comment:strong expression lateral thoracic cluster   • nucleus (Moore et al., 2002) Comment:strong expression embryonic stage 13   dorsal abdominal cluster   • nucleus (Moore et al., 2002) Comment:strong expression dorsal thoracic cluster   • nucleus (Moore et al., 2002) Comment:strong expression lateral abdominal cluster   • nucleus (Moore et al., 2002) Comment:weak expression lateral thoracic cluster   • nucleus (Moore et al., 2002) Comment:weak expression ventral abdominal cluster   • nucleus (Moore et al., 2002) Comment:strong expression ventral thoracic cluster   • nucleus (Moore et al., 2002) Comment:strong expression embryonic stage 14 -- 15   thecogen cell of dorsal thoracic cluster   • nucleus (Moore et al., 2002) thecogen cell of dorsal abdominal cluster   • nucleus (Moore et al., 2002) embryonic stage 14 -- 17   eo neuron of dorsal thoracic cluster   • nucleus (Moore et al., 2002) eo neuron of dorsal abdominal cluster   • nucleus (Moore et al., 2002) western blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage   organism   (Moore et al., 2002)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0045852    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0045852 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of very low expression. Peak expression observed at stages throughout embryogenesis, during early larval stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0045852 expression level Developmental Stage   Expression Level embryo 00-02hr    19 embryo 02-04hr    250 embryo 04-06hr    454 embryo 06-08hr    775 embryo 08-10hr    1042 embryo 10-12hr    1298 embryo 12-14hr    1217 embryo 14-16hr    1284 embryo 16-18hr    1209 embryo 18-20hr    1100 embryo 20-22hr    911 embryo 22-24hr    813 larva L1    567 larva L2    405 larva L3 12hr old    209 larva L3 puffstage 1-2    309 larva L3 puffstage 3-6    398 larva L3 puffstage 7-9    389 white prepupae new    347 white prepupae 12hr    317 white prepupae 24hr    398 pupae 2d postWPP    256 pupae 3d postWPP    159 pupae 4d postWPP    234 adult male 01day    292 adult male 05day    378 adult male 30day    380 adult female 01day    150 adult female 05day    69 adult female 30day    59 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    19 embryo 02-04hr  (250) embryo 04-06hr  (454) embryo 06-08hr  (775) embryo 08-10hr  (1042) embryo 10-12hr  (1298) embryo 12-14hr  (1217) embryo 14-16hr  (1284) embryo 16-18hr  (1209) embryo 18-20hr  (1100) embryo 20-22hr  (911) embryo 22-24hr  (813) larva L1  (567) larva L2  (405) larva L3 12hr old  (209) larva L3 puffstage 1-2  (309) larva L3 puffstage 3-6  (398) larva L3 puffstage 7-9  (389) white prepupae new  (347) white prepupae 12hr  (317) white prepupae 24hr  (398) pupae 2d postWPP  (256) pupae 3d postWPP  (159) pupae 4d postWPP  (234) adult male 01day  (292) adult male 05day  (378) adult male 30day  (380) adult female 01day  (150) adult female 05day    69 adult female 30day    59 Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    19 embryo 02-04hr    250 embryo 04-06hr    454 embryo 06-08hr    775 embryo 08-10hr    1042 embryo 10-12hr    1298 embryo 12-14hr    1217 embryo 14-16hr    1284 embryo 16-18hr    1209 embryo 18-20hr    1100 embryo 20-22hr    911 embryo 22-24hr    813 larva L1    567 larva L2    405 larva L3 12hr old    209 larva L3 puffstage 1-2    309 larva L3 puffstage 3-6    398 larva L3 puffstage 7-9    389 white prepupae new    347 white prepupae 12hr    317 white prepupae 24hr    398 pupae 2d postWPP    256 pupae 3d postWPP    159 pupae 4d postWPP    234 adult male 01day    292 adult male 05day    378 adult male 30day    380 adult female 01day    150 adult female 05day    69 adult female 30day    59 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    19 embryo 02-04hr    250 embryo 04-06hr    454 embryo 06-08hr    775 embryo 08-10hr    1042 embryo 10-12hr    1298 embryo 12-14hr    1217 embryo 14-16hr    1284 embryo 16-18hr    1209 embryo 18-20hr    1100 embryo 20-22hr    911 embryo 22-24hr    813 larva L1    567 larva L2    405 larva L3 12hr old    209 larva L3 puffstage 1-2    309 larva L3 puffstage 3-6    398 larva L3 puffstage 7-9    389 white prepupae new    347 white prepupae 12hr    317 white prepupae 24hr    398 pupae 2d postWPP    256 pupae 3d postWPP    159 pupae 4d postWPP    234 adult male 01day    292 adult male 05day    378 adult male 30day    380 adult female 01day    150 adult female 05day    69 adult female 30day    59 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    19 embryo 02-04hr    250 embryo 04-06hr    454 embryo 06-08hr    775 embryo 08-10hr    1042 embryo 10-12hr    1298 embryo 12-14hr    1217 embryo 14-16hr    1284 embryo 16-18hr    1209 embryo 18-20hr    1100 embryo 20-22hr    911 embryo 22-24hr    813 larva L1    567 larva L2    405 larva L3 12hr old    209 larva L3 puffstage 1-2    309 larva L3 puffstage 3-6    398 larva L3 puffstage 7-9    389 white prepupae new    347 white prepupae 12hr    317 white prepupae 24hr    398 pupae 2d postWPP    256 pupae 3d postWPP    159 pupae 4d postWPP    234 adult male 01day    292 adult male 05day    378 adult male 30day    380 adult female 01day    150 adult female 05day    69 adult female 30day    59 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0045852 expression level Developmental Stage   Expression Level embryo 00-02hr    19 embryo 02-04hr    250 embryo 04-06hr    454 embryo 06-08hr    775 embryo 08-10hr    1042 embryo 10-12hr    1298 embryo 12-14hr    1217 embryo 14-16hr    1284 embryo 16-18hr    1209 embryo 18-20hr    1100 embryo 20-22hr    911 embryo 22-24hr    813 larva L1    567 larva L2    405 larva L3 12hr old    209 larva L3 puffstage 1-2    309 larva L3 puffstage 3-6    398 larva L3 puffstage 7-9    389 white prepupae new    347 white prepupae 12hr    317 white prepupae 24hr    398 pupae 2d postWPP    256 pupae 3d postWPP    159 pupae 4d postWPP    234 adult male 01day    292 adult male 05day    378 adult male 30day    380 adult female 01day    150 adult female 05day    69 adult female 30day    59 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    19 embryo 02-04hr  (250) embryo 04-06hr  (454) embryo 06-08hr  (775) embryo 08-10hr  (1042) embryo 10-12hr  (1298) embryo 12-14hr  (1217) embryo 14-16hr  (1284) embryo 16-18hr  (1209) embryo 18-20hr  (1100) embryo 20-22hr  (911) embryo 22-24hr  (813) larva L1  (567) larva L2  (405) larva L3 12hr old  (209) larva L3 puffstage 1-2  (309) larva L3 puffstage 3-6  (398) larva L3 puffstage 7-9  (389) white prepupae new  (347) white prepupae 12hr  (317) white prepupae 24hr  (398) pupae 2d postWPP  (256) pupae 3d postWPP  (159) pupae 4d postWPP  (234) adult male 01day  (292) adult male 05day  (378) adult male 30day  (380) adult female 01day  (150) adult female 05day    69 adult female 30day    59 Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    19 embryo 02-04hr    250 embryo 04-06hr    454 embryo 06-08hr    775 embryo 08-10hr    1042 embryo 10-12hr    1298 embryo 12-14hr    1217 embryo 14-16hr    1284 embryo 16-18hr    1209 embryo 18-20hr    1100 embryo 20-22hr    911 embryo 22-24hr    813 larva L1    567 larva L2    405 larva L3 12hr old    209 larva L3 puffstage 1-2    309 larva L3 puffstage 3-6    398 larva L3 puffstage 7-9    389 white prepupae new    347 white prepupae 12hr    317 white prepupae 24hr    398 pupae 2d postWPP    256 pupae 3d postWPP    159 pupae 4d postWPP    234 adult male 01day    292 adult male 05day    378 adult male 30day    380 adult female 01day    150 adult female 05day    69 adult female 30day    59 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    19 embryo 02-04hr    250 embryo 04-06hr    454 embryo 06-08hr    775 embryo 08-10hr    1042 embryo 10-12hr    1298 embryo 12-14hr    1217 embryo 14-16hr    1284 embryo 16-18hr    1209 embryo 18-20hr    1100 embryo 20-22hr    911 embryo 22-24hr    813 larva L1    567 larva L2    405 larva L3 12hr old    209 larva L3 puffstage 1-2    309 larva L3 puffstage 3-6    398 larva L3 puffstage 7-9    389 white prepupae new    347 white prepupae 12hr    317 white prepupae 24hr    398 pupae 2d postWPP    256 pupae 3d postWPP    159 pupae 4d postWPP    234 adult male 01day    292 adult male 05day    378 adult male 30day    380 adult female 01day    150 adult female 05day    69 adult female 30day    59 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    19 embryo 02-04hr    250 embryo 04-06hr    454 embryo 06-08hr    775 embryo 08-10hr    1042 embryo 10-12hr    1298 embryo 12-14hr    1217 embryo 14-16hr    1284 embryo 16-18hr    1209 embryo 18-20hr    1100 embryo 20-22hr    911 embryo 22-24hr    813 larva L1    567 larva L2    405 larva L3 12hr old    209 larva L3 puffstage 1-2    309 larva L3 puffstage 3-6    398 larva L3 puffstage 7-9    389 white prepupae new    347 white prepupae 12hr    317 white prepupae 24hr    398 pupae 2d postWPP    256 pupae 3d postWPP    159 pupae 4d postWPP    234 adult male 01day    292 adult male 05day    378 adult male 30day    380 adult female 01day    150 adult female 05day    69 adult female 30day    59 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0045852    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0045852 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Heatmap Tissue   Expression Level Larval Central Nervous System no informative data Larval Midgut no informative data Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain no informative data Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut no informative data Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart no informative data Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis no informative data Adult Male Accessory Gland no informative data Adult Carcass     modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_11
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0045852 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG31753-RA;CG15907-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable hamLA00005 viable, with Scer\GAL4pnr-MD237 hamGD14761 Other Phenotypes Allele neuroanatomy defective ham1 hamdsRNA.cMa neuroanatomy defective, with Scer\GAL4109(2)80 hamScer\UAS.cMa neuroanatomy defective, with Scer\GAL4elav.PLu hamScer\UAS.cMa neuroanatomy defective, with Scer\GAL4pros.PMG hamScer\UAS.cMa visible, with Scer\GAL4neur-GAL4-A101 hamScer\UAS.cMa visible, with Scer\GAL4sca-109-68 hamScer\UAS.cMa Phenotype manifest in Allele abdominal ventral sensillum campaniformium vc4a ham1 chaeta, with Scer\GAL4neur-GAL4-A101 hamScer\UAS.cMa dorsal abdominal cluster ham1 hamdsRNA.cMa embryonic/larval nervous system ham1 embryonic/larval nervous system | somatic clone ham1 eo neuron ham1 hamdsRNA.cMa eo neuron | somatic clone ham1 eo neuron | supernumerary, with Scer\GAL4pros.PMG hamScer\UAS.cMa multidendritic neuron ham1 multidendritic neuron, with Scer\GAL4109(2)80 hamScer\UAS.cMa multidendritic neuron, with Scer\GAL4elav.PLu hamScer\UAS.cMa multidendritic neuron, with Scer\GAL4pros.PMG hamScer\UAS.cMa multidendritic neuron | ectopic | somatic clone ham1 multidendritic neuron | somatic clone ham1 multidendritic neuron | supernumerary ham1 hamdsRNA.cMa tormogen cell, with Scer\GAL4neur-GAL4-A101 hamScer\UAS.cMa tormogen cell, with Scer\GAL4sca-109-68 hamScer\UAS.cMa tormogen cell | adult stage | ectopic | somatic clone ham1 trichogen cell, with Scer\GAL4neur-GAL4-A101 hamScer\UAS.cMa trichogen cell, with Scer\GAL4sca-109-68 hamScer\UAS.cMa trichogen cell | adult stage | ectopic | somatic clone ham1 trichogen cell | ectopic | larval stage ham1 trichogen cell | supernumerary ham1
Classical Alleles ( 5 )
For All Classical Alleles Show
Allele of ham Class Mutagen Stocks Known lesion hamLA00005 P-element activity 2 -- hamf00703 piggyBac transposase 1 -- ham1 loss of function allele 0 Yes hamf07061 0 -- hamunspecified 0 --
Alleles Carried on Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Allele of ham Class Mutagen Stocks Known lesion hamGD14761 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes hamHMS00470 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes hamJF02270 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes hamKK102224 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes hamdsRNA.cMa in vitro construct - RNAi 0 Yes hamGD4127 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes hamScer\UAS.cMa in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Ab(2)2.3-65S12 (Brumby et al., 2004) Df(2L)2.3-59S16 (Brumby et al., 2004) Df(2L)Exel7071 (Ryder, 2004.4.26) Df(2L)Exel7072 (Ryder, 2004.4.26) Df(2L)OD15 (Moore et al., 2002)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD4127} NA P{GD14761} NA P{KK102224} NA P{TRiP.HMS00470} NA P{TRiP.JF02270} NA P{UAS-ham.M} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{WH}hamf00703 NA PBac{WH}hamf07061 NA insertion of mobile activating element P{Mae-UAS.6.11}hamLA00005 NA
Gene Ontology: Function, Process & Cellular Component ( 9 unique terms )
Terms Based on Experimental Evidence ( 6 terms )
Molecular Function
CV term Evidence References
sequence-specific DNA binding transcription factor activity inferred from mutant phenotype (assigned by UniProtKB) (Moore et al., 2004)
Biological Process
CV term Evidence References
neuron development inferred from mutant phenotype (Parrish et al., 2006) regulation of dendrite morphogenesis inferred from mutant phenotype (assigned by UniProtKB) (Moore et al., 2002, Moore et al., 2004) regulation of transcription, DNA-dependent inferred from mutant phenotype (assigned by UniProtKB) (Moore et al., 2004) sensory organ development inferred from mutant phenotype (assigned by UniProtKB) (Moore et al., 2002, Moore et al., 2004)
Cellular Component
CV term Evidence References
nucleus inferred from direct assay (assigned by UniProtKB) (Moore et al., 2002)
Terms Based on Predictions or Assertions ( 5 terms )
Molecular Function
CV term Evidence References
nucleic acid binding inferred from electronic annotation with InterPro:IPR013087 (FlyBase Curators et al., 2004-) sequence-specific DNA binding transcription factor activity non-traceable author statement (Moore, 2002.10.17) zinc ion binding inferred from electronic annotation with InterPro:IPR007087, InterPro:IPR015880 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
dendrite morphogenesis traceable author statement (Grueber and Jan, 2004)
Cellular Component
CV term Evidence References
nucleus traceable author statement (Gao and Bogert, 2003)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions ham allele Gene References ham1 N (Moore et al., 2004)
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0045852 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35135
Orthologs
Genome-wide drosophilid orthologs	Dana\GF14488 Dere\GG21684 Dgri\GH11200 Dmoj\GI18122 Dper\GL18691 Dpse\GA25755 Dsec\GM17063 Dsim\GD21809 Dvir\GJ17745 Dwil\GK15535 Dyak\GE12705
Curated drosophilid orthologs	Dpse\GA16448
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AGAP008232-PA Anopheles gambiae (mosquito) XP_001121599.1 Apis mellifera (honey bee) XP_001945435.1 Acyrthosiphon pisum (pea aphid) 126318 Branchiostoma floridae (lancelet) BGIBMGA008996-PA Bombyx mori (silk worm) ENSBTAP00000007717 Bos taurus (bovine) CN01392 Caenorhabditis brenneri (small nematode) CBP28872 Caenorhabditis briggsae (nematode) CE03572 Caenorhabditis elegans (worm) ENSCAFP00000021630 Canis familiaris (dog) ENSCAFP00000028796 Canis familiaris (dog) JA12978 Caenorhabditis japonica (nematode) CPIJ012100 Culex pipens (mosquito) ENSCPOP00000012219 Cavia porcellus (guinea pig) RP01564 Caenorhabditis remanei (nematode) ENSCSAVP00000007598 Ciona savignyi (sedentary tunicate) 223464 Capitella spl (polychaete worm) ENSECAP00000018770 Equus caballus (horse) ENSGACP00000009660 Gymnopilus aculeatus (mushroom) ENSGALP00000015350 Gallus gallus (chicken) ENSP00000264674 Homo sapiens (human) ISCW010781-PA Ixodes scapularis (deer tick) ENSMODP00000034995 Monodelphis domestica (gray opossum) ENSMMUP00000040663 Macaca mulatta (rhesus monkey) ENSMUSP00000101263 Mus musculus (house mouse) XP_001606323.1 Nasonia vitripennis (wasp) ENSOANP00000012088 Ornithorynchus anatinus (platypus) ENSORLP00000005644 Oryzias latipes (japanese killifish) PHUM000709-PA Pediculus humanus (human body louse) ENSPPYP00000015958 Pongo pygmaeus (orangutan) 144145 Phytophthora sojae (root rot pathogen) ENSPTRP00000026847 Pan troglodytes (chimpanzee) ENSRNOP00000017023 Rattus norvegicus (norway rat) XP_975197.2 Tribolium castaneum (red flour beetle) ENSTNIP00000014935 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000019907 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000026332 Xenopus tropicalis (frog) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV11692 LH12619 ISCW010781 FBgn0230429 FBgn0193229 FBgn0171975 JGI_V11_14809 FBgn0247132 FBgn0156292 FBgn0117531 CPIJ012100 CPIJ008060 BGIBMGA008996 ACYPI007819 GB12947 AGAP008232 AAEL017229 PB19688 PHUM496070 GLEAN_06417
Stocks & Reagents
Stocks Listed in FlyBase ( 8 )
Bloomington	26728 y1 v1; P{TRiP.JF02270}attP2 22209 y1 w*; P{Mae-UAS.6.11}hamLA00005 32470 y1 sc* v1; P{TRiP.HMS00470}attP2
Harvard	- PBac{WH}hamf00703
Kyoto	120005 y* w*; P{Mae-UAS.6.11}hamLA00005
VDRC	v107275 P{KK102224}VIE-260B v40763 w1118; P{GD14761}v40763 v40762 w1118; P{GD14761}v40762/TM3
Genomic Clones ( 1 )
	BACR07J05 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 18 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE64434
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones	71517 102533 119767 144903 164408 214257.30 CK01.74D.D9 CK01.93D.C3 CK01.113A.G1 EK002019 EK002419 EK058615 EK073110 EK094404 EK225921 EK251018 IP05835
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0045852 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35135
Antibody Information
	polyclonal (Moore et al., 2002)
Other Information
Discoverer
Etymology
	The gene is named "hamlet" after the "To be or not to be" soliloquy in the Shakespeare play of the same name, as mutations of the gene appear to affect determination of cells descended from the IIB precursor of the external sensory organ precursor cell lineage. (Moore et al., 2002)
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: CG10568 CG15906 (FlyBase Genome Annotators, 2002-2003) Source for merge of: hamlet CG15907 CG31753 (FlyBase, 1996-)
Additional comments	"ham" does not correspond to "l(2)36Fd"; mutations in these two genes complement each other. (Brumby et al., 2004) Annotations CG15907 and CG31753 merged as CG31753 (which corresponds to ham) in release 3.2 of the genome annotation. (FlyBase Genome Annotators, 2004) Annotations CG10568 and CG15906 merged as CG31753 in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003) Source for merge of hamlet CG15907 CG31753 was sequence comparison (date:021223). (FlyBase, 1996-)
Other Comments
	dsRNA has been made from templates generated with primers directed against this gene. ham RNAi results in supernumerary class I neurons. (Parrish et al., 2006) In ham mutant external sensory organs, the internal cell types (external sensory neuron and thecogen) are converted to external cell types via a cousin-cousin cell-fate respecification event. (Moore et al., 2004) Loss of ham function in hamlet mutant embryos results in a switch of neural fate; the ES neuron is transformed into an MD neuron. (Moore and Jan, 2002) ham acts as a binary genetic switch that acts to repress a multiple dendritic arbor (multidendritic neuron fate) and promotes single-dendrite morphology (external sensory neuron fate). (Moore et al., 2002) Mutants show altered cell fates in the external sensory organ lineage; the fate of the IIIb daughters is altered, with the sensory neuron being converted to a second multiple dendritic neuron and the glia being converted to a second trichogen. (Moore and Jan, 2001)
External Crossreferences & Linkouts
Sequence Crossreferences
	DDBJ / EMBL / GenBank • AAF53703 AAN86032 AY166585 CZ483172 DU708599 Entrez Gene - A searchable database of RefSeq genes. • 35135 UniProtKB/Swiss-Prot • Q8I7Z8
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Zinc finger, C2H2 (IPR007087) Zinc finger, C2H2-type/integrase, DNA-binding (IPR013087) Zinc finger, C2H2-like (IPR015880)
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0045852 DRSC - Results from RNAi screens. • FBgn0045852 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0045852 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG31753-RA;CG15907-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0045852 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35135 InParanoid A subset of ortholog calls from InParanoid. • AGAP008232-PA Anopheles gambiae (mosquito) XP_001121599.1 Apis mellifera (honey bee) XP_001945435.1 Acyrthosiphon pisum (pea aphid) 126318 Branchiostoma floridae (lancelet) BGIBMGA008996-PA Bombyx mori (silk worm) ENSBTAP00000007717 Bos taurus (bovine) CN01392 Caenorhabditis brenneri (small nematode) CBP28872 Caenorhabditis briggsae (nematode) CE03572 Caenorhabditis elegans (worm) ENSCAFP00000021630 Canis familiaris (dog) ENSCAFP00000028796 Canis familiaris (dog) JA12978 Caenorhabditis japonica (nematode) CPIJ012100 Culex pipens (mosquito) ENSCPOP00000012219 Cavia porcellus (guinea pig) RP01564 Caenorhabditis remanei (nematode) ENSCSAVP00000007598 Ciona savignyi (sedentary tunicate) 223464 Capitella spl (polychaete worm) ENSECAP00000018770 Equus caballus (horse) ENSGACP00000009660 Gymnopilus aculeatus (mushroom) ENSGALP00000015350 Gallus gallus (chicken) ENSP00000264674 Homo sapiens (human) ISCW010781-PA Ixodes scapularis (deer tick) ENSMODP00000034995 Monodelphis domestica (gray opossum) ENSMMUP00000040663 Macaca mulatta (rhesus monkey) ENSMUSP00000101263 Mus musculus (house mouse) XP_001606323.1 Nasonia vitripennis (wasp) ENSOANP00000012088 Ornithorynchus anatinus (platypus) ENSORLP00000005644 Oryzias latipes (japanese killifish) PHUM000709-PA Pediculus humanus (human body louse) ENSPPYP00000015958 Pongo pygmaeus (orangutan) 144145 Phytophthora sojae (root rot pathogen) ENSPTRP00000026847 Pan troglodytes (chimpanzee) ENSRNOP00000017023 Rattus norvegicus (norway rat) XP_975197.2 Tribolium castaneum (red flour beetle) ENSTNIP00000014935 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000019907 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000026332 Xenopus tropicalis (frog) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /dbzhnsky/hamlet1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35135 modMine - Data generated by the modENCODE project. • FBgn0045852 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV11692 LH12619 ISCW010781 FBgn0230429 FBgn0193229 FBgn0171975 JGI_V11_14809 FBgn0247132 FBgn0156292 FBgn0117531 CPIJ012100 CPIJ008060 BGIBMGA008996 ACYPI007819 GB12947 AGAP008232 AAEL017229 PB19688 PHUM496070 GLEAN_06417
Synonyms & Secondary IDs ( 8 )
Reported As
Symbol Synonym	CG10568   CG15906   CG15907   CG31753 (Perkins et al., 2009.3.31, Keleman et al., 2009.8.5, Ni et al., 2010.12.1) ham (Hoskins et al., 2005, Crozatier and Vincent, 2008, Andrews et al., 2009) Ham (Rebeiz et al., 2011)
Name Synonym	hamlet (Moore and Jan, 2001, Parrish et al., 2006, Gao and Bogert, 2003) Hamlet (Crozatier and Vincent, 2008, Andrews et al., 2009, Rebeiz et al., 2011)
Secondary FlyBase IDs
	FBgn0032710 FBgn0032711 FBgn0040991 FBgn0051753
References ( 37 )
Generate a list of	All references relating to this gene ( 37 )
List References by type	Research paper  ( 9 ) Supplementary material  ( 3 ) Review  ( 4 ) Personal communication to FlyBase  ( 7 ) Abstract  ( 4 ) FlyBase analysis  ( 5 ) DNA/RNA sequence record  ( 1 ) Computer file  ( 2 ) Automatic genome annotation  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 1 )
	Rebeiz et al., 2011, Development 138(2): 215--225 Notch regulates numb: integration of conditional and autonomous cell fate specification. [FBrf0212636] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...