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Gene Dmel\CG32276

General Information
Symbol	Dmel\CG32276	Species	D. melanogaster
Name		Annotation symbol	CG32276
Feature type	protein_coding_gene	FlyBase ID	FBgn0047135
Gene Model Status	Current	Stock availability	2 publicly available
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	63B11-63B11	Sequence location	3L:3,197,862..3,199,043 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	This gene is referred to in FlyBase by the symbol Dmel\CG32276 (FBgn0047135). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. Based on sequence similarity, it is predicted to be involved in the biological process: protein modification process. 2 alleles are reported. No phenotypic data is available. It has 3 annotated transcripts and 3 annotated polypeptides. Protein features are: Endoplasmic reticulum, stress-associated Ramp4. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of high expression. Peak expression observed at stages throughout embryogenesis, at stages throughout the larval period, at stages throughout the pupal period, in stages of adults of both sexes. Summary of FlyAtlas Anatomical Expression Data: High levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1634918_a_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of CG32276. Gene sequence location is 3L:3197862..3199043.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2011_10
FB2012_01	Sequence features HFA08027 BKN45762 BKN28603
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 63B11-63B11   Limits computationally determined from genome sequence between P{PZ}l(3)0680306803 and P{PZ}kst01318
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\CG32276 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	BLASTX homology to ribosome associated membrane protein 4
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) CG32276-RA FBtr0073106  NM_167998   861   64 CG32276-RB FBtr0073107  NM_167999   735   64 CG32276-RC FBtr0306014     643   65
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein CG32276-PA   FBpp0072968   7.1   64   11.01     NP_728830   AAN11540 CG32276-PB   FBpp0072969   7.1   64   11.01     NP_728831   AAN11541 CG32276-PC   FBpp0297156   7.7   65   11.40
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Endoplasmic reticulum, stress-associated Ramp4 (IPR010580)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA990729   AE014296 AAN11540   AE014296 AAN11541   AI109816   AI109817   AI113600   AI259839   AI294884   AI297696   AI456769   AI513920   AY071239 AAL48861   BE975611   BI162885   BI170430   BI228771   BI229990   BI231970   BI355004   BI355764   BP549275   BP551479   BP555298   BQ103315   BQ103348   BT120259   CL527806   CO179744   CO179799   CO182311   CO183109   CO183168   CO184395   CO188268   CO189754   CO191576   CO191975   CO284907   CO296180   CO297283   CO312130   CO314040   CO332755   CO335843   CO335962   EC065343   EC076195   EC079296   EC080269   EC087344   EC224552   EC226737   EC263193   EC267739   ES689874   ES689960   EV577760   EV577816   EV578057   EV579384   EV581302   EV584786   EV592541   EV593323
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q8SYY6
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0047135    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0047135 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of high expression. Peak expression observed at stages throughout embryogenesis, at stages throughout the larval period, at stages throughout the pupal period, in stages of adults of both sexes. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0047135 expression level Developmental Stage   Expression Level embryo 00-02hr    8883 embryo 02-04hr    13997 embryo 04-06hr    11841 embryo 06-08hr    16348 embryo 08-10hr    25841 embryo 10-12hr    30588 embryo 12-14hr    36799 embryo 14-16hr    17476 embryo 16-18hr    18238 embryo 18-20hr    10148 embryo 20-22hr    11599 embryo 22-24hr    14322 larva L1    11878 larva L2    29802 larva L3 12hr old    21655 larva L3 puffstage 1-2    24133 larva L3 puffstage 3-6    20129 larva L3 puffstage 7-9    25719 white prepupae new    25380 white prepupae 12hr    13172 white prepupae 24hr    14705 pupae 2d postWPP    20769 pupae 3d postWPP    10886 pupae 4d postWPP    5414 adult male 01day    11805 adult male 05day    8888 adult male 30day    8756 adult female 01day    7621 adult female 05day    13127 adult female 30day    12499 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (8883) embryo 02-04hr  (13997) embryo 04-06hr  (11841) embryo 06-08hr  (16348) embryo 08-10hr  (25841) embryo 10-12hr  (30588) embryo 12-14hr  (36799) embryo 14-16hr  (17476) embryo 16-18hr  (18238) embryo 18-20hr  (10148) embryo 20-22hr  (11599) embryo 22-24hr  (14322) larva L1  (11878) larva L2  (29802) larva L3 12hr old  (21655) larva L3 puffstage 1-2  (24133) larva L3 puffstage 3-6  (20129) larva L3 puffstage 7-9  (25719) white prepupae new  (25380) white prepupae 12hr  (13172) white prepupae 24hr  (14705) pupae 2d postWPP  (20769) pupae 3d postWPP  (10886) pupae 4d postWPP  (5414) adult male 01day  (11805) adult male 05day  (8888) adult male 30day  (8756) adult female 01day  (7621) adult female 05day  (13127) adult female 30day  (12499) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (8883) embryo 02-04hr  (13997) embryo 04-06hr  (11841) embryo 06-08hr  (16348) embryo 08-10hr  (25841) embryo 10-12hr  (30588) embryo 12-14hr  (36799) embryo 14-16hr  (17476) embryo 16-18hr  (18238) embryo 18-20hr  (10148) embryo 20-22hr  (11599) embryo 22-24hr  (14322) larva L1  (11878) larva L2  (29802) larva L3 12hr old  (21655) larva L3 puffstage 1-2  (24133) larva L3 puffstage 3-6  (20129) larva L3 puffstage 7-9  (25719) white prepupae new  (25380) white prepupae 12hr  (13172) white prepupae 24hr  (14705) pupae 2d postWPP  (20769) pupae 3d postWPP  (10886) pupae 4d postWPP  (5414) adult male 01day  (11805) adult male 05day  (8888) adult male 30day  (8756) adult female 01day  (7621) adult female 05day  (13127) adult female 30day  (12499) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    8883 embryo 02-04hr  (13997) embryo 04-06hr  (11841) embryo 06-08hr  (16348) embryo 08-10hr  (25841) embryo 10-12hr  (30588) embryo 12-14hr  (36799) embryo 14-16hr  (17476) embryo 16-18hr  (18238) embryo 18-20hr  10148 embryo 20-22hr  (11599) embryo 22-24hr  (14322) larva L1  (11878) larva L2  (29802) larva L3 12hr old  (21655) larva L3 puffstage 1-2  (24133) larva L3 puffstage 3-6  (20129) larva L3 puffstage 7-9  (25719) white prepupae new  (25380) white prepupae 12hr  (13172) white prepupae 24hr  (14705) pupae 2d postWPP  (20769) pupae 3d postWPP  (10886) pupae 4d postWPP    5414 adult male 01day  (11805) adult male 05day    8888 adult male 30day    8756 adult female 01day    7621 adult female 05day  (13127) adult female 30day  (12499) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    8883 embryo 02-04hr    13997 embryo 04-06hr    11841 embryo 06-08hr    16348 embryo 08-10hr    25841 embryo 10-12hr    30588 embryo 12-14hr    36799 embryo 14-16hr    17476 embryo 16-18hr    18238 embryo 18-20hr    10148 embryo 20-22hr    11599 embryo 22-24hr    14322 larva L1    11878 larva L2    29802 larva L3 12hr old    21655 larva L3 puffstage 1-2    24133 larva L3 puffstage 3-6    20129 larva L3 puffstage 7-9    25719 white prepupae new    25380 white prepupae 12hr    13172 white prepupae 24hr    14705 pupae 2d postWPP    20769 pupae 3d postWPP    10886 pupae 4d postWPP    5414 adult male 01day    11805 adult male 05day    8888 adult male 30day    8756 adult female 01day    7621 adult female 05day    13127 adult female 30day    12499 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0047135 expression level Developmental Stage   Expression Level embryo 00-02hr    8883 embryo 02-04hr    13997 embryo 04-06hr    11841 embryo 06-08hr    16348 embryo 08-10hr    25841 embryo 10-12hr    30588 embryo 12-14hr    36799 embryo 14-16hr    17476 embryo 16-18hr    18238 embryo 18-20hr    10148 embryo 20-22hr    11599 embryo 22-24hr    14322 larva L1    11878 larva L2    29802 larva L3 12hr old    21655 larva L3 puffstage 1-2    24133 larva L3 puffstage 3-6    20129 larva L3 puffstage 7-9    25719 white prepupae new    25380 white prepupae 12hr    13172 white prepupae 24hr    14705 pupae 2d postWPP    20769 pupae 3d postWPP    10886 pupae 4d postWPP    5414 adult male 01day    11805 adult male 05day    8888 adult male 30day    8756 adult female 01day    7621 adult female 05day    13127 adult female 30day    12499 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (8883) embryo 02-04hr  (13997) embryo 04-06hr  (11841) embryo 06-08hr  (16348) embryo 08-10hr  (25841) embryo 10-12hr  (30588) embryo 12-14hr  (36799) embryo 14-16hr  (17476) embryo 16-18hr  (18238) embryo 18-20hr  (10148) embryo 20-22hr  (11599) embryo 22-24hr  (14322) larva L1  (11878) larva L2  (29802) larva L3 12hr old  (21655) larva L3 puffstage 1-2  (24133) larva L3 puffstage 3-6  (20129) larva L3 puffstage 7-9  (25719) white prepupae new  (25380) white prepupae 12hr  (13172) white prepupae 24hr  (14705) pupae 2d postWPP  (20769) pupae 3d postWPP  (10886) pupae 4d postWPP  (5414) adult male 01day  (11805) adult male 05day  (8888) adult male 30day  (8756) adult female 01day  (7621) adult female 05day  (13127) adult female 30day  (12499) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (8883) embryo 02-04hr  (13997) embryo 04-06hr  (11841) embryo 06-08hr  (16348) embryo 08-10hr  (25841) embryo 10-12hr  (30588) embryo 12-14hr  (36799) embryo 14-16hr  (17476) embryo 16-18hr  (18238) embryo 18-20hr  (10148) embryo 20-22hr  (11599) embryo 22-24hr  (14322) larva L1  (11878) larva L2  (29802) larva L3 12hr old  (21655) larva L3 puffstage 1-2  (24133) larva L3 puffstage 3-6  (20129) larva L3 puffstage 7-9  (25719) white prepupae new  (25380) white prepupae 12hr  (13172) white prepupae 24hr  (14705) pupae 2d postWPP  (20769) pupae 3d postWPP  (10886) pupae 4d postWPP  (5414) adult male 01day  (11805) adult male 05day  (8888) adult male 30day  (8756) adult female 01day  (7621) adult female 05day  (13127) adult female 30day  (12499) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    8883 embryo 02-04hr  13997 embryo 04-06hr  11841 embryo 06-08hr  16348 embryo 08-10hr  (25841) embryo 10-12hr  (30588) embryo 12-14hr  (36799) embryo 14-16hr  17476 embryo 16-18hr  18238 embryo 18-20hr  10148 embryo 20-22hr  11599 embryo 22-24hr  14322 larva L1  11878 larva L2  (29802) larva L3 12hr old  (21655) larva L3 puffstage 1-2  (24133) larva L3 puffstage 3-6  20129 larva L3 puffstage 7-9  (25719) white prepupae new  (25380) white prepupae 12hr  13172 white prepupae 24hr  14705 pupae 2d postWPP  (20769) pupae 3d postWPP  10886 pupae 4d postWPP    5414 adult male 01day  11805 adult male 05day    8888 adult male 30day    8756 adult female 01day    7621 adult female 05day  13127 adult female 30day  12499 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    8883 embryo 02-04hr    13997 embryo 04-06hr    11841 embryo 06-08hr    16348 embryo 08-10hr    25841 embryo 10-12hr    30588 embryo 12-14hr    36799 embryo 14-16hr    17476 embryo 16-18hr    18238 embryo 18-20hr    10148 embryo 20-22hr    11599 embryo 22-24hr    14322 larva L1    11878 larva L2    29802 larva L3 12hr old    21655 larva L3 puffstage 1-2    24133 larva L3 puffstage 3-6    20129 larva L3 puffstage 7-9    25719 white prepupae new    25380 white prepupae 12hr    13172 white prepupae 24hr    14705 pupae 2d postWPP    20769 pupae 3d postWPP    10886 pupae 4d postWPP    5414 adult male 01day    11805 adult male 05day    8888 adult male 30day    8756 adult female 01day    7621 adult female 05day    13127 adult female 30day    12499 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0047135    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0047135 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: High levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0047135 expression level Tissue   Expression Level Larval Central Nervous System    1049.975 Larval Midgut    3100 Larval Hindgut    1990.3 Larval Malpighian Tubules    1290 Larval Fat Body    6939.3 Larval Salivary Gland    7365.6 Larval Trachea    3365.1 Larval Carcass    2421.35 Adult Head    2358.5 Adult Eye    1475.7 Adult Brain    891.4 Adult Thoracic-Abdominal Ganglion    964.2 Adult Crop    1572.8 Adult Midgut    3061.9 Adult Hindgut    1208 Adult Malpighian Tubules    1777.5 Adult Fat Body    3284.8 Adult Salivary Gland    10450.5 Adult Heart    2553.325 Adult VirginFemale Spermatheca    4349.6 Adult InseminatedFemale Spermatheca    4804.8 Adult Ovary    1535.5 Adult Testis    576.5 Adult Male Accessory Gland    7756.7 Adult Carcass    2020.3 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1049.975) Larval Midgut  (3100) Larval Hindgut  (1990.3) Larval Malpighian Tubules  (1290) Larval Fat Body  (6939.3) Larval Salivary Gland  (7365.6) Larval Trachea  (3365.1) Larval Carcass  (2421.35) Adult Head  (2358.5) Adult Eye  (1475.7) Adult Brain  (891.4) Adult Thoracic-Abdominal Ganglion  (964.2) Adult Crop  (1572.8) Adult Midgut  (3061.9) Adult Hindgut  (1208) Adult Malpighian Tubules  (1777.5) Adult Fat Body  (3284.8) Adult Salivary Gland  (10450.5) Adult Heart  (2553.325) Adult VirginFemale Spermatheca  (4349.6) Adult InseminatedFemale Spermatheca  (4804.8) Adult Ovary  (1535.5) Adult Testis  (576.5) Adult Male Accessory Gland  (7756.7) Adult Carcass  (2020.3) Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  1049.975 Larval Midgut  (3100) Larval Hindgut  (1990.3) Larval Malpighian Tubules  (1290) Larval Fat Body  (6939.3) Larval Salivary Gland  (7365.6) Larval Trachea  (3365.1) Larval Carcass  (2421.35) Adult Head  (2358.5) Adult Eye  (1475.7) Adult Brain    891.4 Adult Thoracic-Abdominal Ganglion    964.2 Adult Crop  (1572.8) Adult Midgut  (3061.9) Adult Hindgut  (1208) Adult Malpighian Tubules  (1777.5) Adult Fat Body  (3284.8) Adult Salivary Gland  (10450.5) Adult Heart  (2553.325) Adult VirginFemale Spermatheca  (4349.6) Adult InseminatedFemale Spermatheca  (4804.8) Adult Ovary  (1535.5) Adult Testis    576.5 Adult Male Accessory Gland  (7756.7) Adult Carcass  (2020.3) Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1049.975 Larval Midgut    3100 Larval Hindgut    1990.3 Larval Malpighian Tubules    1290 Larval Fat Body    6939.3 Larval Salivary Gland    7365.6 Larval Trachea    3365.1 Larval Carcass    2421.35 Adult Head    2358.5 Adult Eye    1475.7 Adult Brain    891.4 Adult Thoracic-Abdominal Ganglion    964.2 Adult Crop    1572.8 Adult Midgut    3061.9 Adult Hindgut    1208 Adult Malpighian Tubules    1777.5 Adult Fat Body    3284.8 Adult Salivary Gland    10450.5 Adult Heart    2553.325 Adult VirginFemale Spermatheca    4349.6 Adult InseminatedFemale Spermatheca    4804.8 Adult Ovary    1535.5 Adult Testis    576.5 Adult Male Accessory Gland    7756.7 Adult Carcass    2020.3 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0047135 expression level Tissue   Expression Level Larval Central Nervous System    1049.975 Larval Midgut    3100 Larval Hindgut    1990.3 Larval Malpighian Tubules    1290 Larval Fat Body    6939.3 Larval Salivary Gland    7365.6 Larval Trachea    3365.1 Larval Carcass    2421.35 Adult Head    2358.5 Adult Eye    1475.7 Adult Brain    891.4 Adult Thoracic-Abdominal Ganglion    964.2 Adult Crop    1572.8 Adult Midgut    3061.9 Adult Hindgut    1208 Adult Malpighian Tubules    1777.5 Adult Fat Body    3284.8 Adult Salivary Gland    10450.5 Adult Heart    2553.325 Adult VirginFemale Spermatheca    4349.6 Adult InseminatedFemale Spermatheca    4804.8 Adult Ovary    1535.5 Adult Testis    576.5 Adult Male Accessory Gland    7756.7 Adult Carcass    2020.3 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1049.975) Larval Midgut  (3100) Larval Hindgut  (1990.3) Larval Malpighian Tubules  (1290) Larval Fat Body  (6939.3) Larval Salivary Gland  (7365.6) Larval Trachea  (3365.1) Larval Carcass  (2421.35) Adult Head  (2358.5) Adult Eye  (1475.7) Adult Brain  (891.4) Adult Thoracic-Abdominal Ganglion  (964.2) Adult Crop  (1572.8) Adult Midgut  (3061.9) Adult Hindgut  (1208) Adult Malpighian Tubules  (1777.5) Adult Fat Body  (3284.8) Adult Salivary Gland  (10450.5) Adult Heart  (2553.325) Adult VirginFemale Spermatheca  (4349.6) Adult InseminatedFemale Spermatheca  (4804.8) Adult Ovary  (1535.5) Adult Testis  576.5 Adult Male Accessory Gland  (7756.7) Adult Carcass  (2020.3) Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  1049.975 Larval Midgut  (3100) Larval Hindgut  (1990.3) Larval Malpighian Tubules  1290 Larval Fat Body  (6939.3) Larval Salivary Gland  (7365.6) Larval Trachea  (3365.1) Larval Carcass  (2421.35) Adult Head  (2358.5) Adult Eye  1475.7 Adult Brain    891.4 Adult Thoracic-Abdominal Ganglion    964.2 Adult Crop  1572.8 Adult Midgut  (3061.9) Adult Hindgut  1208 Adult Malpighian Tubules  (1777.5) Adult Fat Body  (3284.8) Adult Salivary Gland  (10450.5) Adult Heart  (2553.325) Adult VirginFemale Spermatheca  (4349.6) Adult InseminatedFemale Spermatheca  (4804.8) Adult Ovary  1535.5 Adult Testis    576.5 Adult Male Accessory Gland  (7756.7) Adult Carcass  (2020.3) Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1049.975 Larval Midgut    3100 Larval Hindgut    1990.3 Larval Malpighian Tubules    1290 Larval Fat Body    6939.3 Larval Salivary Gland    7365.6 Larval Trachea    3365.1 Larval Carcass    2421.35 Adult Head    2358.5 Adult Eye    1475.7 Adult Brain    891.4 Adult Thoracic-Abdominal Ganglion    964.2 Adult Crop    1572.8 Adult Midgut    3061.9 Adult Hindgut    1208 Adult Malpighian Tubules    1777.5 Adult Fat Body    3284.8 Adult Salivary Gland    10450.5 Adult Heart    2553.325 Adult VirginFemale Spermatheca    4349.6 Adult InseminatedFemale Spermatheca    4804.8 Adult Ovary    1535.5 Adult Testis    576.5 Adult Male Accessory Gland    7756.7 Adult Carcass    2020.3 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    2358.5   NA  Eye    1475.7   NA  Brain    891.4   1049.975  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    964.2   NA  Crop    1572.8   3100  Midgut    3061.9   1990.3  Hindgut    1208   1290  Malpighian Tubules    1777.5   6939.3  Fat Body    3284.8   7365.6  Salivary Gland    10450.5   NA  Heart    2553.325   3365.1  Trachea    NA   NA  VirginFemale Spermatheca    4349.6   NA  InseminatedFemale Spermatheca    4804.8   NA  Ovary    1535.5   NA  Testis    576.5   NA  Male Accessory Gland    7756.7   2421.35  Carcass    2020.3 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_09
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0047135 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG32276-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for CG32276 Stages(s) 1-3 Stages(s) 4-6 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable CG32276EY05425 viable, with Scer\GAL4pnr-MD237 CG32276GD16921 Sterility Allele fertile CG32276EY05425 Phenotype manifest in Allele
Classical Alleles ( 1 )
For All Classical Alleles Show
Allele of CG32276 Class Mutagen Stocks Known lesion CG32276EY05425 P-element activity 1 --
Alleles Carried on Transgenic Constructs ( 1 )
For All Alleles Carried on Transgenic Constructs Show
Allele of CG32276 Class Mutagen Stocks Known lesion CG32276GD16921 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD16921} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}CG32276EY05425 NA
Gene Ontology: Function, Process & Cellular Component ( 3 unique terms )
Terms Based on Experimental Evidence ( 0 terms )
Terms Based on Predictions or Assertions ( 3 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
protein modification process inferred from sequence or structural similarity with NCBI:NP_055260 (FlyBase Genome Annotators, 2004)
Cellular Component
CV term Evidence References
endoplasmic reticulum inferred from sequence or structural similarity with NCBI:NP_055260 (FlyBase Genome Annotators, 2004) ribosome inferred from sequence or structural similarity with NCBI:NP_055260 (FlyBase Genome Annotators, 2004)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions CG32276 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 75573 DroID - A comprehensive database of gene and protein interactions. • FBgn0047135 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 251645
Orthologs
Genome-wide drosophilid orthologs	Dana\GF24382 Dere\GG14279 Dgri\GH15253 Dmoj\GI11962 Dper\GL25245 Dpse\GA16804 Dsec\GM14072 Dsim\GD13346 Dvir\GJ12186 Dwil\GK25584 Dyak\GE20707
Curated drosophilid orthologs	Dpse\GA16804
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL012684-PA Aedes aegypti (mosquito) AGAP001548-PA Anopheles gambiae (mosquito) XP_001119845.1 Apis mellifera (honey bee) XP_001947005.1 Acyrthosiphon pisum (pea aphid) At1g27330.1 Arabidopsis thaliana (wall cress) At1g27350.1 Arabidopsis thaliana (wall cress) XP_001891615.1 Brugia malayi (roundworm nematode) BGIBMGA004406-PA Bombyx mori (silk worm) ENSBTAP00000012000 Bos taurus (bovine) CBP27690 Caenorhabditis briggsae (nematode) CE31064 Caenorhabditis elegans (worm) ENSCAFP00000006869 Canis familiaris (dog) ENSCINP00000022609 Ciona intestinalis (sea squirt) JA01820 Caenorhabditis japonica (nematode) cgd2_140 Cryptosporidium parvum (protozoan parasite) CPIJ001216 Culex pipens (mosquito) ENSCPOP00000010135 Cavia porcellus (guinea pig) 192290 Chlamidomonas reinhardtii (green alga) RP28419 Caenorhabditis remanei (nematode) 175858 Capitella spl (polychaete worm) 304099 Daphnia pulex (daphnia) ENSDARP00000090232 Danio rerio (zebra fish) ENSGACP00000009157 Gymnopilus aculeatus (mushroom) 64670 Hypsipyla robusta (cedar shoot borer) ENSP00000239944 Homo sapiens (human) ISCW016512-PA Ixodes scapularis (deer tick) 193286 Lottia gigantea (owl limpet) ENSMODP00000018636 Monodelphis domestica (gray opossum) ENSMMUP00000023276 Macaca mulatta (rhesus monkey) ENSMUSP00000068508 Mus musculus (house mouse) 169659 Nematostella vectensis (starlet anemone) XP_001606906.1 Nasonia vitripennis (wasp) LOC_Os12g32950.1 Oryza sativa (asian rice) PHUM010298-PA Pediculus humanus (human body louse) PP17358 Pristionchus pacificus (nematode) 205447 Physcomitrella patens (moss) ENSPPYP00000006070 Pongo pygmaeus (orangutan) 717321 Populus trichocarpa (black cottonwood) ENSPTRP00000026732 Pan troglodytes (chimpanzee) ENSRNOP00000017868 Rattus norvegicus (norway rat) 148940 Sorghum bicolor (sorghum) 4892779 Sorghum bicolor (sorghum) Smp_089570 Schistosoma mansoni (trematode parasite) XP_001182842.1 Strongylocentrotus purpuratus (purple sea urchin) XP_001200035.1 Strongylocentrotus purpuratus (purple sea urchin) 64256 Trichoplax adhaerens (metazoa) XP_970109.1 Tribolium castaneum (red flour beetle) ENSTNIP00000022999 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000046168 Takifugu rubripes (pufferfish Takifugu) 3811.m00990 Tetrahymena thermophila (ciliate protozoa) ENSXETP00000056277 Xenopus tropicalis (frog) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB11020 NV15087 LH23451 ISCW016512 FBgn0238011 FBgn0227543 FBgn0199438 FBgn0185062 FBgn0169001 JGI_V11_304099 FBgn0076818 FBgn0162829 FBgn0134719 FBgn0122728 FBgn0106540 FBgn0101376 CPIJ001216 BGIBMGA004406 ACYPI002437 GB19388 AGAP001548 AAEL012684 PHUM495990 GLEAN_03121
Stocks & Reagents
Stocks Listed in FlyBase ( 2 )
Bloomington	16370 y1 w67c23; P{EPgy2}CG32276EY05425
VDRC	v49568 w1118; P{GD16921}v49568/TM3
Genomic Clones ( 1 )
	BACR48E19 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 58 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE27904
Other clones	MIP17161
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH09181 GH09182 GH09967 GM29178 GM32217 LD34570 LD36890 LD45957 LP03345 LP08364 LP12059 RE02152 RE11873 RE25156 RE26414
Other clones	77890 83076 101578 108231 158780 277508 542790 542900 bs44g03 CK01.24B.F12 Dmel_dig_000484_0598_0465 Dmel_dig_003591_0706_2296 Dmel_dig_004908_0622_1676 Dmel_dig_005550_0712_0695 Dmel_dig_006474_0633_1906 Dmel_dig_020201_0711_3195 Dmel_neb_012359_0622_0314 Dmel_neb_015867_0700_2085 EC00126 EC00226 EC22429 EC24580 EC25032 EC28031 EC31438 EC32518 EK038848 EK045104 EK167445 EK213002 EK216549 EK227922 EK234755 EK304455 EN11332 EN11553 FGM004F05 FGM213B02 FGM234E06 UUGC0718 UUGC0751
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0047135 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 251645
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	New annotation (CG32276) in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_167998 NM_167999 RefSeq (Proteins) • NP_728830 NP_728831 DDBJ / EMBL / GenBank • AAL48861 AAN11540 AAN11541 AY071239 BI229990 CL527806 Entrez Gene - A searchable database of RefSeq genes. • 251645 UniProtKB/TrEMBL • Q8SYY6
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Endoplasmic reticulum, stress-associated Ramp4 (IPR010580)
Linkouts
	BioGRID - A database of protein and genetic interactions • 75573 DroID - A comprehensive database of gene and protein interactions. • FBgn0047135 DRSC - Results from RNAi screens. • FBgn0047135 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0047135 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG32276-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0047135 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 251645 InParanoid A subset of ortholog calls from InParanoid. • AAEL012684-PA Aedes aegypti (mosquito) AGAP001548-PA Anopheles gambiae (mosquito) XP_001119845.1 Apis mellifera (honey bee) XP_001947005.1 Acyrthosiphon pisum (pea aphid) At1g27330.1 Arabidopsis thaliana (wall cress) At1g27350.1 Arabidopsis thaliana (wall cress) XP_001891615.1 Brugia malayi (roundworm nematode) BGIBMGA004406-PA Bombyx mori (silk worm) ENSBTAP00000012000 Bos taurus (bovine) CBP27690 Caenorhabditis briggsae (nematode) CE31064 Caenorhabditis elegans (worm) ENSCAFP00000006869 Canis familiaris (dog) ENSCINP00000022609 Ciona intestinalis (sea squirt) JA01820 Caenorhabditis japonica (nematode) cgd2_140 Cryptosporidium parvum (protozoan parasite) CPIJ001216 Culex pipens (mosquito) ENSCPOP00000010135 Cavia porcellus (guinea pig) 192290 Chlamidomonas reinhardtii (green alga) RP28419 Caenorhabditis remanei (nematode) 175858 Capitella spl (polychaete worm) 304099 Daphnia pulex (daphnia) ENSDARP00000090232 Danio rerio (zebra fish) ENSGACP00000009157 Gymnopilus aculeatus (mushroom) 64670 Hypsipyla robusta (cedar shoot borer) ENSP00000239944 Homo sapiens (human) ISCW016512-PA Ixodes scapularis (deer tick) 193286 Lottia gigantea (owl limpet) ENSMODP00000018636 Monodelphis domestica (gray opossum) ENSMMUP00000023276 Macaca mulatta (rhesus monkey) ENSMUSP00000068508 Mus musculus (house mouse) 169659 Nematostella vectensis (starlet anemone) XP_001606906.1 Nasonia vitripennis (wasp) LOC_Os12g32950.1 Oryza sativa (asian rice) PHUM010298-PA Pediculus humanus (human body louse) PP17358 Pristionchus pacificus (nematode) 205447 Physcomitrella patens (moss) ENSPPYP00000006070 Pongo pygmaeus (orangutan) 717321 Populus trichocarpa (black cottonwood) ENSPTRP00000026732 Pan troglodytes (chimpanzee) ENSRNOP00000017868 Rattus norvegicus (norway rat) 148940 Sorghum bicolor (sorghum) 4892779 Sorghum bicolor (sorghum) Smp_089570 Schistosoma mansoni (trematode parasite) XP_001182842.1 Strongylocentrotus purpuratus (purple sea urchin) XP_001200035.1 Strongylocentrotus purpuratus (purple sea urchin) 64256 Trichoplax adhaerens (metazoa) XP_970109.1 Tribolium castaneum (red flour beetle) ENSTNIP00000022999 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000046168 Takifugu rubripes (pufferfish Takifugu) 3811.m00990 Tetrahymena thermophila (ciliate protozoa) ENSXETP00000056277 Xenopus tropicalis (frog) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 251645 modMine - Data generated by the modENCODE project. • FBgn0047135 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB11020 NV15087 LH23451 ISCW016512 FBgn0238011 FBgn0227543 FBgn0199438 FBgn0185062 FBgn0169001 JGI_V11_304099 FBgn0076818 FBgn0162829 FBgn0134719 FBgn0122728 FBgn0106540 FBgn0101376 CPIJ001216 BGIBMGA004406 ACYPI002437 GB19388 AGAP001548 AAEL012684 PHUM495990 GLEAN_03121
Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	BcDNA:RE27904   CG32276 (Isogai et al., 2007, Birch-Machin et al., 2005)
Name Synonym
Secondary FlyBase IDs
	FBgn0052276
References ( 12 )
Research paper	Isogai et al., 2007, Genes Dev. 21(22): 2936--2949 Transcription of histone gene cluster by differential core-promoter factors. [FBrf0202929] Birch-Machin et al., 2005, GenomeBiol. 6(7): R63 Genomic analysis of heat-shock factor targets in Drosophila. [FBrf0187687]
Supplementary material	The modENCODE Consortium, 2010, Science 330(6012): DataS14: Gene co-expression clusters. [FBrf0213979] Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]
Personal communication to FlyBase	Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327] Ashburner and Marygold, 2004.7.2, Ribosomal proteins. Ribosomal proteins. [FBrf0178764]
FlyBase analysis	FlyBase Genome Annotators, 2004, Changes affecting gene model number or type in release 3.2 of the annotated D.melanogaster genome. Changes affecting gene model number or type in release 3.2 of the annotated D.melanogaster genome. [FBrf0166453] FlyBase Genome Annotators, 2002-2003, Changes affecting gene model number or type in releases 3.0 and 3.1 of the annotated D.melanogaster genome. Changes affecting gene model number or type in releases 3.0 and 3.1 of the annotated D.melanogaster genome. [FBrf0148886] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0125078]