FB2021_01, released Feb 18, 2021

Gene: Dmel\metro

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General Information
Symbol
Dmel\metro
Species
D. melanogaster
Name
menage a trois
Annotation Symbol
CG30021
Feature Type
protein_coding_gene
FlyBase ID
FBgn0050021
Gene Model Status
Current
Stock Availability
10 publicly available
Gene Snapshot
menage a trois (metro) encodes controls the expansion of larval neuromuscular junctions (NMJs) and the proper establishment of synaptic boutons. At NMJs Metro associates with the adaptor protein encoded by veli to stabilize the product of dlg1 in a trimeric scaffold complex that limits the size of active zones and glutamate receptor fields. [Date last reviewed: 2019-03-14]
Other Summaries
Also Known As

skiff

Key Links
Genomic Location
Cytogenetic map
47E1-47E1
Sequence location
2R:11,226,458..11,232,516 [-]
Recombination map

2-64

RefSeq locus
NT_033778 REGION:11226458..11232516
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (2 terms)
Molecular Function (0 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
involved_in regulation of synaptic growth at neuromuscular junction
inferred from mutant phenotype
(Bachmann et al., 2010)
Terms Based on Predictions or Assertions (0 terms)
Cellular Component (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
located_in subsynaptic reticulum
inferred from direct assay
(Bachmann et al., 2010)
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Summary (Interactive Fly)

a MAGUK serving as a major binding partner of perisynaptic Dlg at larval neuromuscular junctions - stabilizes Dlg in a complex with adaptor protein Lin-7 - controls NMJ expansion and proper establishment of synaptic boutons

Gene Model and Products
Number of Transcripts
4
Number of Unique Polypeptides
4

Please see the JBrowse view of Dmel\metro for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.49

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
metro-RA
FBtr0088196
NM_136798
2782
595
metro-RB
FBtr0114569
NM_001144170
2665
556
metro-RC
FBtr0114570
NM_001144171
2752
585
metro-RD
FBtr0339484
NM_001299380
2635
546
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
metro-PA
FBpp0087292
66.7
595
5.43
NP_610642
AAF58707
metro-PB
FBpp0113061
62.6
556
5.18
NP_001137642
ACL83096
metro-PC
FBpp0113062
65.7
585
5.52
NP_001137643
ACL83097
metro-PD
FBpp0308566
61.7
546
5.25
NP_001286309
AHN56107
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\metro using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
third instar larval stage
embryonic/larval neuromuscular junction
(Bachmann et al., 2010)
embryonic/larval neuromuscular junction
(Bachmann et al., 2010)
embryonic/larval neuromuscular junction
(Bachmann et al., 2010)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
day 7 of adulthood -- day 49 of adulthood
adult heart
(Cammarato et al., 2011)
adult stage
adult head
(Aradska et al., 2015)
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
third instar larval stage
embryonic/larval hypodermal muscle
(Bachmann et al., 2010)
Additional Descriptive Data

metro protein is detected in the type I boutons and in the subsynaptic reticulum, in the larval neuromuscular junction. Its expression colocalises with the expression of veli protein.

(Bachmann et al., 2010)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in subsynaptic reticulum
inferred from direct assay
(Bachmann et al., 2010)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\metro in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 8 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of metro
Transgenic constructs containing regulatory region of metro
Aberrations (Deficiencies and Duplications) ( 1 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
viable
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
Sterility
Allele
fertile
Other Phenotypes
Allele
abnormal neuroanatomy
Phenotype manifest in
Allele
neuromuscular junction
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (21)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\MPP7
13 of 15
Yes
Yes
Hsap\MPP3
11 of 15
No
Yes
Hsap\MPP4
8 of 15
No
Yes
Hsap\MPP5
3 of 15
No
No
Hsap\MPP1
2 of 15
No
No
Hsap\MPP2
2 of 15
No
No
Hsap\MPP6
2 of 15
No
No
Hsap\CARD10
1 of 15
No
Yes
Hsap\CARD11
1 of 15
No
Yes
Hsap\CARD14
1 of 15
No
Yes
Hsap\CASK
1 of 15
No
No
2  
Hsap\DLG1
1 of 15
No
No
Hsap\DLG2
1 of 15
No
No
Hsap\DLG3
1 of 15
No
No
Hsap\DLG4
1 of 15
No
No
2  
Hsap\DLG5
1 of 15
No
No
Hsap\GUK1
1 of 15
No
No
1  
Hsap\LRGUK
1 of 15
No
No
Hsap\MAGI3
1 of 15
No
No
Hsap\PATJ
1 of 15
No
No
Hsap\PDZD2
1 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (21)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Mpp7
12 of 15
Yes
Yes
Mmus\Mpp3
11 of 15
No
Yes
Mmus\Mpp4
6 of 15
No
Yes
Mmus\Mpp5
3 of 15
No
No
Mmus\Mpp1
2 of 15
No
No
Mmus\Mpp2
2 of 15
No
No
Mmus\Mpp6
2 of 15
No
No
Mmus\Card10
1 of 15
No
Yes
Mmus\Card11
1 of 15
No
Yes
Mmus\Card14
1 of 15
No
Yes
Mmus\Cask
1 of 15
No
No
Mmus\Dlg1
1 of 15
No
No
Mmus\Dlg2
1 of 15
No
No
Mmus\Dlg3
1 of 15
No
No
Mmus\Dlg4
1 of 15
No
No
Mmus\Dlg5
1 of 15
No
No
Mmus\Guk1
1 of 15
No
No
Mmus\Lrguk
1 of 15
No
No
Mmus\Magi3
1 of 15
No
No
Mmus\Patj
1 of 15
No
No
Mmus\Pdzd2
1 of 15
No
No
Rattus norvegicus (Norway rat) (18)
Rnor\Mpp7
11 of 13
Yes
Yes
Rnor\Mpp3
10 of 13
No
Yes
Rnor\Mpp4
6 of 13
No
Yes
Rnor\Mpp5
3 of 13
No
No
Rnor\Mpp6
2 of 13
No
No
Rnor\Card10
1 of 13
No
Yes
Rnor\Card11
1 of 13
No
Yes
Rnor\Card14
1 of 13
No
Yes
Rnor\Dlg1
1 of 13
No
No
Rnor\Dlg2
1 of 13
No
No
Rnor\Dlg3
1 of 13
No
No
Rnor\Dlg4
1 of 13
No
No
Rnor\Dlg5
1 of 13
No
No
Rnor\Guk1
1 of 13
No
No
Rnor\Lrguk
1 of 13
No
No
Rnor\Magi3
1 of 13
No
No
Rnor\Mpp2
1 of 13
No
No
Rnor\Patj
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (14)
Xtro\mpp7
11 of 12
Yes
Yes
Xtro\mpp5
3 of 12
No
No
Xtro\mpp1
2 of 12
No
No
Xtro\mpp2
2 of 12
No
No
Xtro\mpp6
2 of 12
No
No
Xtro\card14
1 of 12
No
Yes
Xtro\cask
1 of 12
No
No
Xtro\dlg1
1 of 12
No
No
Xtro\dlg2
1 of 12
No
No
Xtro\dlg4
1 of 12
No
No
Xtro\dlg5
1 of 12
No
No
Xtro\guk1
1 of 12
No
No
Xtro\magi3
1 of 12
No
No
Xtro\mpp4
1 of 12
No
Yes
Danio rerio (Zebrafish) (29)
Drer\mpp7a
12 of 15
Yes
Yes
Drer\mpp3b
10 of 15
No
Yes
Drer\mpp3a
9 of 15
No
Yes
Drer\mpp4a
6 of 15
No
Yes
Drer\mpp4b
6 of 15
No
Yes
Drer\mpp7b
6 of 15
No
Yes
Drer\LOC556594
4 of 15
No
Yes
Drer\mpp1
2 of 15
No
No
Drer\mpp2a
2 of 15
No
No
Drer\mpp2b
2 of 15
No
No
Drer\mpp5a
2 of 15
No
No
Drer\mpp5b
2 of 15
No
No
Drer\mpp6a
2 of 15
No
No
Drer\mpp6b
2 of 15
No
No
Drer\card11
1 of 15
No
Yes
Drer\caska
1 of 15
No
No
Drer\dlg1
1 of 15
No
No
Drer\dlg3
1 of 15
No
No
Drer\dlg4b
1 of 15
No
No
Drer\dlg4
1 of 15
No
No
Drer\dlg5a
1 of 15
No
No
Drer\dlg5b.1
1 of 15
No
No
Drer\guk1b
1 of 15
No
No
Drer\im:7140055
1 of 15
No
Yes
Drer\LOC564220
1 of 15
No
No
Drer\LOC100534780
1 of 15
No
Yes
Drer\LOC100537443
1 of 15
No
No
Drer\lrguk
1 of 15
No
No
Drer\magi3a
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (8)
Cele\magu-1
14 of 15
Yes
Yes
Cele\magu-2
3 of 15
No
No
Cele\dlg-1
1 of 15
No
No
Cele\guk-1
1 of 15
No
No
Cele\lin-2
1 of 15
No
No
Cele\magi-1
1 of 15
No
No
Cele\magu-3
1 of 15
No
No
Cele\magu-4
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (3)
Atha\GK-1
1 of 9
Yes
No
Atha\MAPKKK6
1 of 9
Yes
Yes
Atha\MAPKKK7
1 of 9
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\GUK1
2 of 15
Yes
No
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\SPBC1198.05
1 of 12
Yes
No
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091904HE )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00002653
Drosophila simulans
Dsim\GD25940
Drosophila sechellia
Dsec\GM20471
Drosophila erecta
Dere\GG22696
Drosophila yakuba
Dyak\GE13049
Drosophila ananassae
Dana\GF12435
Drosophila pseudoobscura pseudoobscura
Dpse\GA15582
Drosophila persimilis
Dper\GL20641
Drosophila willistoni
Dwil\GK20656
Drosophila virilis
Dvir\GJ15026
Drosophila mojavensis
Dmoj\GI19661
Drosophila grimshawi
Dgri\GH21953
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091502OH )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17014563
Glossina morsitans
Tsetse fly
Gmor\GMOY006357
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_02034
Mayetiola destructor
Hessian fly
Mdes\Mdes012869
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL010294
Anopheles darlingi
American malaria mosquito
Adar\ADAC005861
Anopheles gambiae
Malaria mosquito
Agam\AGAP010230
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ003839
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W02FX )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA000711
Danaus plexippus
Monarch butterfly
Dple\DPOGS206150-PA
Danaus plexippus
Monarch butterfly
Dple\DPOGS206151-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL013473
Heliconius melpomene
Postman butterfly
Hmel\HMEL015061
Apis florea
Little honeybee
Aflo\2624677716
Apis mellifera
Western honey bee
Amel\GB45852
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP22431
Bombus terrestris
Buff-tailed bumblebee
Bter\15214057
Linepithema humile
Argentine ant
Lhum\LH18517
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:003190
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG000966
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_11106
Tribolium castaneum
Red flour beetle
Tcas\TC003628
Pediculus humanus
Human body louse
Phum\PHUM565090
Rhodnius prolixus
Kissing bug
Rpro\RPRC002666
Cimex lectularius
Bed bug
Clec\CLEC000429
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI002650
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_03175
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X02DG )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR013138
Ixodes scapularis
Black-legged tick
Isca\ISCW022002
Stegodyphus mimosarum
African social velvet spider
Smim\KFM61655.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM67965.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM61654.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur24g01670
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_98778
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G065I )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Cask
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Mpp5-2
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Cask_2
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Mpp5-3
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Mpp5
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Mpp6
Strongylocentrotus purpuratus
Purple sea urchin
Spur\SPU_023432gn
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000020496
Ciona intestinalis
Vase tunicate
Cint\LOC100181746
Ciona intestinalis
Vase tunicate
Cint\LOC100179311
Ciona intestinalis
Vase tunicate
Cint\LOC100176249
Gallus gallus
Domestic chicken
Ggal\MPP1
Gallus gallus
Domestic chicken
Ggal\MPP3
Gallus gallus
Domestic chicken
Ggal\MPP4
Gallus gallus
Domestic chicken
Ggal\MPP6
Gallus gallus
Domestic chicken
Ggal\CASK
Gallus gallus
Domestic chicken
Ggal\MPP7
Gallus gallus
Domestic chicken
Ggal\MPP5
Gallus gallus
Domestic chicken
Ggal\MPP2
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (6)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
sdt
5 of 10
CASK
3 of 10
vari
3 of 10
CG11811
1 of 10
dlg1
1 of 10
Dlg5
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    MPP7; membrane palmitoylated protein 7
    13 of 15
    610973
        MPP3; membrane palmitoylated protein 3
        11 of 15
        601114
            MPP4; membrane palmitoylated protein 4
            8 of 15
            606575
                MPP5; membrane palmitoylated protein 5
                3 of 15
                606958
                    Functional Complementation Data
                    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
                    Interactions
                    Summary of Physical Interactions
                    esyN Network Diagram
                    Show neighbor-neighbor interactions:
                    Select Layout:
                    Legend:
                    Protein
                    RNA
                    Selected Interactor(s)
                    Interactions Browser
                    View in Interactions Browser
                    Please see the Physical Interaction reports below for full details
                    RNA-protein
                    Physical Interaction
                    Assay
                    References
                    metro - B52
                    systematic evolution of ligands by exponential enrichment, full identification by DNA sequencing, electrophoretic mobility shift assay, autoradiography
                    (Kim et al., 2003)
                    protein-protein
                    Physical Interaction
                    Assay
                    References
                    metro - dlg1
                    pull down, anti tag western blot
                    (Bachmann et al., 2010)
                    metro - veli
                    pull down, anti tag western blot, two hybrid
                    (Hu et al., 2017.6.13, Bachmann et al., 2010)
                    Summary of Genetic Interactions
                    esyN Network Diagram
                    Starting gene(s)
                    Interaction type
                    Interacting gene(s)
                    Reference
                    Starting gene(s)
                    Interaction type
                    Interacting gene(s)
                    Reference
                    External Data
                    Linkouts
                    BioGRID - A database of protein and genetic interactions.
                    DroID - A comprehensive database of gene and protein interactions.
                    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
                    MIST (protein-protein) - An integrated Molecular Interaction Database
                    Pathways
                    Signaling Pathways (FlyBase)
                    Metabolic Pathways
                    External Data
                    Linkouts
                    Genomic Location and Detailed Mapping Data
                    Chromosome (arm)
                    2R
                    Recombination map

                    2-64

                    Cytogenetic map
                    47E1-47E1
                    Sequence location
                    2R:11,226,458..11,232,516 [-]
                    FlyBase Computed Cytological Location
                    Cytogenetic map
                    Evidence for location
                    47E1-47E1
                    Limits computationally determined from genome sequence between P{EP}shnEP2359&P{EP}shnEP644 and P{PZ}rM547
                    Experimentally Determined Cytological Location
                    Cytogenetic map
                    Notes
                    References
                    47D7-47D7
                    (Bilder, 2002.3.25)
                    Experimentally Determined Recombination Data
                    Location

                    2-64

                    (FlyBase, 2016)

                    2-64.1

                    (Comeron et al., 2012)
                    Left of (cM)
                    Right of (cM)
                    Notes
                    Stocks and Reagents
                    Stocks (10)
                    Genomic Clones (27)
                    cDNA Clones (32)
                     

                    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

                    cDNA clones, fully sequenced
                    BDGP DGC clones
                    Other clones
                    Drosophila Genomics Resource Center cDNA clones

                    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

                    cDNA Clones, End Sequenced (ESTs)
                    BDGP DGC clones
                    Other clones
                    RNAi and Array Information
                    Linkouts
                    DRSC - Results frm RNAi screens
                    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
                    Antibody Information
                    Laboratory Generated Antibodies
                     

                    polyclonal

                    (Bachmann et al., 2010)
                    Commercially Available Antibodies
                     
                    Other Information
                    Relationship to Other Genes
                    Source for database identify of
                    Source for database merge of

                    Source for merge of: CG13219 CG18338

                    (FlyBase Genome Annotators, 2002-2003)
                    Additional comments

                    The CG30021 annotation was previously referred to as "skiff" in FlyBase. However, this name refers to an embryonic dorsal closure phenotype which subsequently turned out to result from a mutation in a separate gene.

                    (Bachmann et al., 2010)

                    FlyBase curator comment: Since the "skiff" name refers to a mutation associated with a separate gene from the CG30021 annotation, the "skiff" synonyms have been removed from the CG30021 gene record and it has been renamed to "metro".

                    (Bachmann et al., 2010)

                    Annotations CG13219 and CG18338 merged as CG30021 in release 3 of the genome annotation.

                    (FlyBase Genome Annotators, 2002-2003)
                    Other Comments

                    Expression is enriched in embryonic gonads.

                    (Shigenobu et al., 2006)

                    dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

                    (Kiger et al., 2003)
                    Origin and Etymology
                    Discoverer
                    Etymology

                    The gene is named "metro" regarding its involvement in a menage a trois with the dlg1 S97 isoform and veli proteins.

                    (Bachmann et al., 2010)
                    Identification
                    External Crossreferences and Linkouts ( 59 )
                    Sequence Crossreferences
                    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                    Other crossreferences
                    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
                    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                    Flygut - An atlas of the Drosophila adult midgut
                    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
                    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                    InterPro - A database of protein families, domains and functional sites
                    KEGG Genes - Molecular building blocks of life in the genomic space.
                    modMine - A data warehouse for the modENCODE project
                    SignaLink - A signaling pathway resource with multi-layered regulatory networks.
                    Linkouts
                    BioGRID - A database of protein and genetic interactions.
                    DPiM - Drosophila Protein interaction map
                    DroID - A comprehensive database of gene and protein interactions.
                    DRSC - Results frm RNAi screens
                    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
                    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                    FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                    FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel
                    FlyMine - An integrated database for Drosophila genomics
                    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
                    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
                    MIST (protein-protein) - An integrated Molecular Interaction Database
                    Synonyms and Secondary IDs (11)
                    Datasets (0)
                    Study focus (0)
                    Experimental Role
                    Project
                    Project Type
                    Title
                    References (47)