FB2021_01, released Feb 18, 2021

Gene: Dmel\Rif1

Open Close
General Information
Symbol
Dmel\Rif1
Species
D. melanogaster
Name
Rap1 interacting factor 1
Annotation Symbol
CG30085
Feature Type
protein_coding_gene
FlyBase ID
FBgn0050085
Gene Model Status
Current
Stock Availability
5 publicly available
Gene Snapshot
Rap1 interacting factor 1 (Rif1) encodes a protein that binds protein phosphatase PP1 and is required for temporal regulation of replication initiation. [Date last reviewed: 2019-03-14]
Other Summaries
Also Known As

dRif1

Key Links
Genomic Location
Cytogenetic map
52C8-52C8
Sequence location
2R:15,864,309..15,869,147 [-]
Recombination map

2-75

RefSeq locus
NT_033778 REGION:15864309..15869147
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (12 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables protein binding
inferred from physical interaction with UniProtKB:Q9VTE2
(assigned by UniProt )
(Munden et al., 2018)
inferred from physical interaction with UniProtKB:P12982
(assigned by UniProt )
(Seller and O'Farrell, 2018, Sreesankar et al., 2015)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (6 terms)
Terms Based on Experimental Evidence (5 terms)
CV Term
Evidence
References
involved_in embryonic development via the syncytial blastoderm
inferred from mutant phenotype
(assigned by UniProt )
(Seller and O'Farrell, 2018)
involved_in negative regulation of DNA-dependent DNA replication
inferred from mutant phenotype
(Munden et al., 2018)
involved_in negative regulation of mitotic cell cycle phase transition
inferred from mutant phenotype
(assigned by UniProt )
(Seller and O'Farrell, 2018)
involved_in regulation of DNA-dependent DNA replication initiation
inferred from mutant phenotype
(assigned by UniProt )
(Seller and O'Farrell, 2018)
inferred from mutant phenotype
(Sreesankar et al., 2015)
involved_in replication fork arrest involved in DNA replication termination
inferred from mutant phenotype
(assigned by UniProt )
(Munden et al., 2018)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in telomere maintenance
inferred from biological aspect of ancestor with PANTHER:PTN001116630
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (5 terms)
Terms Based on Experimental Evidence (5 terms)
CV Term
Evidence
References
located_in chromocenter
inferred from direct assay
(Sreesankar et al., 2015)
located_in chromosome, telomeric region
inferred from direct assay
(Sreesankar et al., 2015)
located_in heterochromatin
inferred from direct assay
(Sreesankar et al., 2015, Sreesankar et al., 2012)
inferred from direct assay
(assigned by UniProt )
(Seller and O'Farrell, 2018)
located_in nuclear replication fork
inferred from direct assay
(Munden et al., 2018)
located_in nucleus
inferred from direct assay
(Sreesankar et al., 2015, Sreesankar et al., 2012)
inferred from direct assay
(assigned by UniProt )
(Seller and O'Farrell, 2018)
inferred from mutant phenotype
(Munden et al., 2018)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
located_in nucleus
inferred from biological aspect of ancestor with PANTHER:PTN001116630
(assigned by GO_Central )
(Gaudet et al., 2011)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the RIF1 family. Highly divergent. (Q9XZ34)
Protein Signatures (InterPro)
Summaries
Protein Function (UniProtKB)
Regulates the timing of initiation of DNA replication (PubMed:29746464, PubMed:30277458, PubMed:26022086). Functions in copy number control by promoting the underreplication of DNA, which is found in many late replicating euchromatic regions of salivary gland polytene chromosomes (PubMed:30277458). Promotes underreplication by localizing to active DNA replication forks in a partially SuUR-dependent manner, and inhibiting replication fork progression (PubMed:30277458). Might also work as an adapter to recruit Pp1-87B to multiple sites on the chromosome and may function with Pp1-87B to mediate underreplication (PubMed:26022086, PubMed:29746464, PubMed:30277458). Plays an essential role in embryonic development, in the transition from larvae to pupae and, probably, in proliferating tissues later on (PubMed:26022086, PubMed:29746464). In embryos, during mid-blastula transition, binds to and selectively delays the replication of large blocks of repetitive DNA satellite sequences during S phase in response to the activity of Cdk1; maternal Rif1 is specifically required for the normal extension of S phase 14 (PubMed:29746464). Unlike mammalian orthologs, does not appear to play a role in DNA damage repair (PubMed:22712556, PubMed:26022086).
(UniProt, Q9XZ34)
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Rif1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.50

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Rif1-RA
FBtr0087306
NM_166120
4605
1416
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
Rif1-PA
FBpp0086441
156.8
1416
6.61
NP_725497
AAM70965
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Interacts with Pp1-87b (PubMed:26022086, PubMed:29746464). Interacts with SuUR (via SNF2-like region) (PubMed:30277458).

(UniProt, Q9XZ34)
Post Translational Modification

Phosphorylated, probably by Cdk1; phosphorylation regulates dissociation from heterochromatin.

(UniProt, Q9XZ34)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Rif1 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism
(Fisher et al., 2012)
embryonic stage 7 -- 8
head mesoderm
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
embryonic stage 9 -- 10
antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
anterior endoderm
(Fisher et al., 2012)
inclusive hindgut primordium
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
ventral ectoderm
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 11 -- 12
anterior midgut primordium
(Fisher et al., 2012)
central brain primordium
(Fisher et al., 2012)
hindgut proper primordium
(Fisher et al., 2012)
Malpighian tubule primordium
(Fisher et al., 2012)
posterior midgut primordium
(Fisher et al., 2012)
salivary gland body primordium
(Fisher et al., 2012)
ventral epidermis primordium
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 13 -- 16
embryonic brain
(Fisher et al., 2012)
embryonic hindgut
(Fisher et al., 2012)
embryonic Malpighian tubule
(Fisher et al., 2012)
embryonic/larval midgut
(Fisher et al., 2012)
ventral nerve cord
(Fisher et al., 2012)
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage

Comment: abundant

(Sreesankar et al., 2015)
adult stage | female
(Sreesankar et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 4
(Sreesankar et al., 2015)
adult stage | female
ovary
(Sreesankar et al., 2015)
oogenesis
follicle cell
(Sreesankar et al., 2015)
nurse cell
(Sreesankar et al., 2015)
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage

Comment: abundant

(Sreesankar et al., 2015)
adult stage | female
ovary
(Sreesankar et al., 2015)
larval stage
eye disc
(Sreesankar et al., 2015)
wing disc
(Sreesankar et al., 2015)
embryonic/larval central nervous system
(Sreesankar et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in chromocenter
inferred from direct assay
(Sreesankar et al., 2015)
located_in chromosome, telomeric region
inferred from direct assay
(Sreesankar et al., 2015)
located_in heterochromatin
inferred from direct assay
(Sreesankar et al., 2015, Sreesankar et al., 2012)
inferred from direct assay
(assigned by UniProt )
(Seller and O'Farrell, 2018)
located_in nuclear replication fork
inferred from direct assay
(Munden et al., 2018)
located_in nucleus
inferred from direct assay
(Sreesankar et al., 2015, Sreesankar et al., 2012)
inferred from direct assay
(assigned by UniProt )
(Seller and O'Farrell, 2018)
inferred from mutant phenotype
(Munden et al., 2018)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Rif1 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 6 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Rif1
Transgenic constructs containing regulatory region of Rif1
Aberrations (Deficiencies and Duplications) ( 0 )
Inferred from experimentation
Inferred from location
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\RIF1
7 of 15
Yes
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Rif1
8 of 15
Yes
Yes
Rattus norvegicus (Norway rat) (1)
Rnor\Rif1
3 of 13
Yes
Yes
Xenopus tropicalis (Western clawed frog) (1)
Xtro\rif1
5 of 12
Yes
Yes
Danio rerio (Zebrafish) (1)
Drer\rif1
9 of 15
Yes
Yes
Caenorhabditis elegans (Nematode, roundworm) (2)
Cele\F11E6.7
3 of 15
Yes
Yes
Cele\F19H6.3
2 of 15
No
Yes
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\RIF1
2 of 15
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\rif1
4 of 12
Yes
Yes
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091900N4 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00005554
Drosophila simulans
Dsim\GD25592
Drosophila sechellia
Dsec\GM20115
Drosophila erecta
Dere\GG22327
Drosophila yakuba
Dyak\GE14124
Drosophila ananassae
Dana\GF13365
Drosophila pseudoobscura pseudoobscura
Dpse\GA15636
Drosophila persimilis
Dper\GL16842
Drosophila willistoni
Dwil\GK20795
Drosophila virilis
Dvir\GJ22233
Drosophila mojavensis
Dmoj\GI19102
Drosophila grimshawi
Dgri\GH21278
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150AS1 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17021840
Glossina morsitans
Tsetse fly
Gmor\GMOY010870
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_08683
Mayetiola destructor
Hessian fly
Mdes\Mdes009138
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL001322
Anopheles darlingi
American malaria mosquito
Adar\ADAC001300
Anopheles gambiae
Malaria mosquito
Agam\AGAP010393
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ020224
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W00XB )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA006763
Danaus plexippus
Monarch butterfly
Dple\DPOGS208111-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL006219
Apis florea
Little honeybee
Aflo\3310382692
Apis mellifera
Western honey bee
Amel\GB43771
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP17840
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP24350
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP24763
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP20995
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP16368
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP17797
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP12618
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP17681
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP12892
Bombus terrestris
Buff-tailed bumblebee
Bter\15202248
Bombus terrestris
Buff-tailed bumblebee
Bter\15202576
Bombus terrestris
Buff-tailed bumblebee
Bter\15204461
Bombus terrestris
Buff-tailed bumblebee
Bter\15202336
Bombus terrestris
Buff-tailed bumblebee
Bter\15202848
Linepithema humile
Argentine ant
Lhum\LH16569
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:000d35
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG015585
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG015584
Tribolium castaneum
Red flour beetle
Tcas\TC001961
Rhodnius prolixus
Kissing bug
Rpro\RPRC009278
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_13927
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X00VO )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR010906
Ixodes scapularis
Black-legged tick
Isca\ISCW021260
Stegodyphus mimosarum
African social velvet spider
Smim\KFM64336.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM63674.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM62954.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM73328.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM62953.1
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_304874
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G01D4 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\SPU_012553gn
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Rif1
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Rif1L
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Hypp_193
Gallus gallus
Domestic chicken
Ggal\RIF1
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (0)
No records found.
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    RIF1; replication timing regulatory factor 1
    7 of 15
    608952
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        esyN Network Diagram
        Show neighbor-neighbor interactions:
        Select Layout:
        Legend:
        Protein
        RNA
        Selected Interactor(s)
        Interactions Browser
        View in Interactions Browser
        Please see the Physical Interaction reports below for full details
        protein-protein
        Physical Interaction
        Assay
        References
        Rif1 - Pp1-87B
        anti tag coimmunoprecipitation, western blot
        (Seller and O'Farrell, 2018, Sreesankar et al., 2015)
        Rif1 - SuUR
        anti tag coimmunoprecipitation, western blot, Identification by mass spectrometry
        (Munden et al., 2018)
        Rif1 - stumps
        two hybrid
        (Battersby et al., 2003)
        Summary of Genetic Interactions
        esyN Network Diagram
        esyN Network Key:
        Suppression
        Enhancement
        View in Interactions Browser
        Please look at the allele data for full details of the genetic interactions
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Rif1
        enhanceable
        Cdc7
        (Seller and O'Farrell, 2018)
        Rif1
        suppressible
        Pp1-87B
        (Sreesankar et al., 2015)
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Cdc7
        suppressible
        Rif1
        (Seller and O'Farrell, 2018)
        External Data
        Subunit Structure (UniProtKB)
        Interacts with Pp1-87b (PubMed:26022086, PubMed:29746464). Interacts with SuUR (via SNF2-like region) (PubMed:30277458).
        (UniProt, Q9XZ34 )
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        External Data
        Linkouts
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        2R
        Recombination map

        2-75

        Cytogenetic map
        52C8-52C8
        Sequence location
        2R:15,864,309..15,869,147 [-]
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        52C8-52C8
        Limits computationally determined from genome sequence between P{lacW}l(2)k05713k05713 and P{lacW}l(2)k02205k02205&P{PZ}sli05248
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        Experimentally Determined Recombination Data
        Location

        2-75

        (FlyBase, 2016)

        2-79.7

        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (5)
        Genomic Clones (17)
         

        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

        cDNA Clones (54)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
          Drosophila Genomics Resource Center cDNA clones

          For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

          cDNA Clones, End Sequenced (ESTs)
          BDGP DGC clones
          Other clones
          RNAi and Array Information
          Linkouts
          DRSC - Results frm RNAi screens
          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
          Antibody Information
          Laboratory Generated Antibodies
           

          polyclonal

          (Munden et al., 2018, Sreesankar et al., 2012)
          Commercially Available Antibodies
           
          Other Information
          Relationship to Other Genes
          Source for database identify of

          Source for identity of: Rif1 CG30085

          (Sreesankar et al., 2012)
          Source for database merge of
          Additional comments
          Other Comments

          New annotation (CG30085) in release 3 of the genome annotation.

          (FlyBase Genome Annotators, 2002-2003)
          Origin and Etymology
          Discoverer
          Etymology

          Named 'Rif1'/'Rap1 interacting factor 1' after the yeast homolog.

          (Sreesankar et al., 2015)
          Identification
          External Crossreferences and Linkouts ( 30 )
          Sequence Crossreferences
          NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
          GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
          GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
          RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
          UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
          Other crossreferences
          BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
          Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
          Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
          Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
          Flygut - An atlas of the Drosophila adult midgut
          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
          InterPro - A database of protein families, domains and functional sites
          KEGG Genes - Molecular building blocks of life in the genomic space.
          modMine - A data warehouse for the modENCODE project
          Linkouts
          BioGRID - A database of protein and genetic interactions.
          DroID - A comprehensive database of gene and protein interactions.
          DRSC - Results frm RNAi screens
          FLIGHT - Cell culture data for RNAi and other high-throughput technologies
          FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
          FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
          FlyMine - An integrated database for Drosophila genomics
          InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
          MIST (protein-protein) - An integrated Molecular Interaction Database
          Synonyms and Secondary IDs (7)
          Datasets (0)
          Study focus (0)
          Experimental Role
          Project
          Project Type
          Title
          References (38)