FlyBase Gene Report: Dmel\CG30122

General Information

Symbol

Dmel\CG30122

Species

D. melanogaster

Name

Annotation Symbol

CG30122

Feature Type

protein_coding_gene

FlyBase ID

FBgn0050122

Gene Model Status

Current

Stock Availability

7 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

55E3-55E3

Sequence location

2R:18,632,328..18,639,270 [-]

Recombination map

2-86

RefSeq locus

NT_033778 REGION:18632328..18639270

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (1 term)

Molecular Function (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in mRNA splicing, via spliceosome

inferred by curator from GO:0071011

(Herold et al., 2009)

Cellular Component (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\CG30122 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.48

Sequence Ontology: Class of Gene

gene_with_edited_transcript

(Graveley et al., 2011)

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

CG30122-RB

FBtr0086660

NM_137510

4620

1271

CG30122-RC

FBtr0300923

NM_001169725

4623

1272

CG30122-RD

FBtr0300924

NM_001169726

4620

1271

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

CG30122-PB

FBpp0085841

140.5

1271

4.64

NP_611354

AAF57660

CG30122-PC

FBpp0290145

140.6

1272

4.64

NP_001163196

ACZ94468

CG30122-PD

FBpp0290146

140.4

1271

4.64

NP_001163197

ACZ94469

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CG30122 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG30122_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

ventral nerve cord primordium

Comment: reported as ventral nerve cord anlage

(Fisher et al., 2012)

embryonic stage 9 -- 10

central brain primordium

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 11 -- 12

central brain primordium

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

larval ventral nerve cord

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 5 of adulthood & male -- day 7 of adulthood & male

spermatozoon

(Wasbrough et al., 2010)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\CG30122 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\CG30122

View exonic expression by tissue for Dmel\CG30122

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0050122

DRscDB

37146/tissue=All

EMBL-EBI Single Cell Expression Atlas

FBgn0050122

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG30122-RB

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG30122

Flygut - An atlas of the Drosophila adult midgut

FBgn0050122

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 5 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of CG30122

Transgenic constructs containing regulatory region of CG30122

Aberrations (Deficiencies and Duplications) ( 3 )

Inferred from experimentation ( 3 )

Gene disrupted in

Df(2R)edl-S0520

(Schwarz et al., 2018)

Df(2R)Exel7157

(Ryder, 2004.4.26)

Gene partially disrupted in

Df(2R)Ex212

(Murthy et al., 2005)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Scer\GAL4^pnr-MD237

CG30122^GD10000

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (6)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\HNRNPUL1

11 of 15

Yes

Hsap\HNRNPU

10 of 15

Yes

Hsap\HNRNPUL2

8 of 15

Yes

Hsap\HNRNPUL2-BSCL2

1 of 15

Yes

Hsap\RNF123

1 of 15

Hsap\RSPRY1

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (5)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Hnrnpul1

11 of 15

Yes

Mmus\Hnrnpu

11 of 15

Yes

Mmus\Hnrnpul2

7 of 15

Yes

Mmus\Rnf123

1 of 15

Mmus\Rspry1

1 of 15

Rattus norvegicus (Norway rat) (4)

Rnor\Hnrnpul1

9 of 13

Yes

Rnor\Hnrnpu

9 of 13

Yes

Rnor\Hnrnpul2

7 of 13

Yes

Rnor\Rnf123

1 of 13

Xenopus tropicalis (Western clawed frog) (5)

Xtro\hnrnpu

6 of 12

Yes

Xtro\hnrnpul2

5 of 12

Yes

Xtro\hnrnpul1

4 of 12

Yes

Xtro\rnf123

1 of 12

Xtro\rspry1

1 of 12

Danio rerio (Zebrafish) (6)

Drer\hnrnpub

11 of 15

Yes

Drer\hnrnpul1

11 of 15

Yes

Drer\hnrnpua

8 of 15

Yes

Drer\hnrnpul1l

7 of 15

Yes

Drer\si:ch211-107m4.1

6 of 15

Yes

Drer\rspry1

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (3)

Cele\hrpu-1

8 of 15

Yes

Cele\acin-1

1 of 15

Cele\F16A11.1

1 of 15

Arabidopsis thaliana (thale-cress) (1)

Atha\TRO

1 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG0919047K )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA24297

Drosophila persimilis

Dper\GL16796

Drosophila willistoni

Dwil\GK21907

Drosophila virilis

Dvir\GJ21813

Drosophila mojavensis

Dmoj\GI18786

Drosophila grimshawi

Dgri\GH20069

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091504MN )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17018415

Glossina morsitans

Tsetse fly

Gmor\GMOY009352

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_09374

Mayetiola destructor

Hessian fly

Mdes\Mdes008289

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL011089

Anopheles darlingi

American malaria mosquito

Adar\ADAC005484

Anopheles gambiae

Malaria mosquito

Agam\AGAP005305

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ009187

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W07B6 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA000103

Danaus plexippus

Monarch butterfly

Dple\DPOGS205868-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL015003

Apis florea

Little honeybee

Aflo\2478935668

Apis mellifera

Western honey bee

Amel\GB41701

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP12084

Bombus terrestris

Buff-tailed bumblebee

Bter\15207529

Linepithema humile

Argentine ant

Lhum\LH15977

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:001118

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG008716

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_02754

Tribolium castaneum

Red flour beetle

Tcas\TC003021

Pediculus humanus

Human body louse

Phum\PHUM163920

Rhodnius prolixus

Kissing bug

Rpro\RPRC005722

Cimex lectularius

Bed bug

Clec\CLEC005130

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_13217

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0769 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR007274

Ixodes scapularis

Black-legged tick

Isca\ISCW001427

Stegodyphus mimosarum

African social velvet spider

Smim\KFM69215.1

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_262452

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_216229

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_30537

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )

No non-Arthropod Metazoa orthologies identified

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (1)

Gene Symbol

Score

Source

Alignment

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

HNRNPU; heterogeneous nuclear ribonucleoprotein U

model of developmental and epileptic encephalopathy 54

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

CG30122^KK100713

exacerbates neurodegenerative disease

modeled by TBPH^Δ23, TBPH^{dsRNA.UAS.cUa}

(Appocher et al., 2017)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

HNRNPUL1; heterogeneous nuclear ribonucleoprotein U like 1

11 of 15

605800

HNRNPU; heterogeneous nuclear ribonucleoprotein U

10 of 15

602869

DEVELOPMENTAL AND EPILEPTIC ENCEPHALOPATHY 54; DEE54

developmental and epileptic encephalopathy 54

HNRNPUL2; heterogeneous nuclear ribonucleoprotein U like 2

8 of 15

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

CG30122 - Drep2

anti tag coimmunoprecipitation, peptide massfingerprinting

(Andlauer et al., 2014)

CG30122 - InR

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

CG30122 - Myc

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

CG30122 - Pdk1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

CG30122 - Pi3K21B

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

CG30122 - Pi3K92E

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

CG30122 - Pten

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

CG30122 - S6kII

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

CG30122 - Tsc1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

CG30122 - foxo

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

CG30122 - gig

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

CG30122 - kel

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

CG30122 - osk

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Hurd et al., 2016)

CG30122 - rictor

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

TBPH

enhanceable

CG30122

(Appocher et al., 2017)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

62819

DroID - A comprehensive database of gene and protein interactions.

FBgn0050122

MIST (protein-protein) - An integrated Molecular Interaction Database

CG30122

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-86

Cytogenetic map

55E3-55E3

Sequence location

2R:18,632,328..18,639,270 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

55E3-55E3

Limits computationally determined from genome sequence between P{EP}EP437 and P{PZ}l(2)03709⁰³⁷⁰⁹

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-86

(FlyBase, 2016)

2-91.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (7)

Bloomington

55209

y¹ sc^* v¹ sev²¹; P{TRiP.HMC03927}attP40

83212

y¹ w^*; TI{CRIMIC.TG4.0}CG30122^{CR00953-TG4.0}/SM6a

Kyoto

123267

w¹¹¹⁸; P{RS3}CG30122^CB-0637-3

125100

w¹¹¹⁸; P{RS5}CG30122^5-HA-1139

105041

w^*; P{GawB}CG30122^NP5613 / CyO

VDRC

v330214

P{VSH330214}attP40

v106984

P{KK100713}VIE-260B

Genomic Clones (27)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (75)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0050122

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0050122

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

37146

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: CG18610 CG5477

(BDGP Project Members, 2000-)

Source for merge of: CG5477 CG18610

(FlyBase, 1996-)

Additional comments

FlyBase curator comment: Correspondence with author Alfonso revealed that 'SAF-A' = 'CG30122'.

(Alfonso and Maggert, 2008)

Annotations CG18610, CG5477 merged as CG30122 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Source for merge of CG5477 CG18610 was a shared cDNA ( date:010720 ).

(FlyBase, 1996-)

Other Comments

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 53 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

37146

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis