FlyBase Gene Report: Dmel\Obp57e

General Information

Symbol

Dmel\Obp57e

Species

D. melanogaster

Name

Odorant-binding protein 57e

Annotation Symbol

CG30145

Feature Type

protein_coding_gene

FlyBase ID

FBgn0050145

Gene Model Status

Current

Stock Availability

3 publicly available

Gene Summary

Odorant-binding protein 57e (Obp57e) encodes a member of the odorant-binding protein family. It is involved in taste perception of middle- to long-chain fatty acids, influencing feeding and oviposition-site preference behavior. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

Obp57d, 57e, DmelObp57e, Dmel\Obp57e

Key Links

Genomic Location

Cytogenetic map

57A5-57A5

Sequence location

2R:20,547,816..20,548,291 [-]

Recombination map

2-91

RefSeq locus

NT_033778 REGION:20547816..20548291

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (6 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables odorant binding

non-traceable author statement

(Hekmat-Scafe et al., 2002)

inferred from sequence or structural similarity with FLYBASE:lush; FB:FBgn0020277

(Vieira and Rozas, 2011)

inferred from electronic annotation with InterPro:IPR006170, InterPro:IPR036728

(InterPro Project Members, 2004-)

Biological Process (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in response to odorant

non-traceable author statement

(Hekmat-Scafe et al., 2002)

involved_in sensory perception of chemical stimulus

inferred from sequence or structural similarity with FLYBASE:lush; FB:FBgn0020277

(Vieira and Rozas, 2011)

involved_in sensory perception of smell

inferred from biological aspect of ancestor with PANTHER:PTN000220814

(GO Reference Genome Project, 2011-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

located_in extracellular region

inferred from sequence model

(Vieira and Rozas, 2011)

is_active_in extracellular space

inferred from biological aspect of ancestor with PANTHER:PTN000220814

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

ODORANT BINDING PROTEINS

Protein Family (UniProt)

Belongs to the PBP/GOBP family. (Q9V938)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

ODORANT BINDING PROTEINS -: Odorant Binding Proteins (OBP) are small (10 to 30 kDa) proteins secreted by auxiliary cells surrounding the olfactory receptor neurons. OBPs are characterized by a specific protein domain that comprises six α-helices joined by three disulfide bonds. Although the full function of the OBPs is not well established, it is believed that they may act as molecular carriers that transport odorants and deliver them to the olfactory receptors located on the sensory neurons. (Adapted from FBrf0205523).

Protein Function (UniProtKB)

Present in the aqueous fluid surrounding olfactory sensory dendrites and are thought to aid in the capture and transport of hydrophobic odorants into and through this fluid.

(UniProt, Q9V938)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Obp57e for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9V938)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

This gene model is based upon FBrf0131082 and gene prediction data.

Gene model reviewed during 5.51

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Obp57e-RA

FBtr0086265

NM_137644

411

136

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Obp57e-PA

FBpp0085577

15.6

136

4.26

Q9V938

NP_611488

AAF57460

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9V938

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Obp57e using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Obp57e_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

NA

Transcript Expression

microinjection

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

photoreceptor neuron

(Xu and Wang, 2016)

Additional Descriptive Data

mRNAs are enriched in photoreceptor cells (found by comparing mRNAs isolated from wild-type (w¹¹¹⁸) heads with gl³ heads or wild-type bodies).

(Xu and Wang, 2016)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

Reporter: P{Obp57e-GFP.M}

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | male

prothoracic leg tarsal taste bristle 4s

(Yasukawa et al., 2010)

prothoracic leg tarsal taste bristle 4c

(Yasukawa et al., 2010)

prothoracic leg tarsal taste bristle 5b

(Yasukawa et al., 2010)

prothoracic leg tarsal taste bristle 5s

(Yasukawa et al., 2010)

mesothoracic leg tarsal taste bristle 5b

(Yasukawa et al., 2010)

mesothoracic leg tarsal taste bristle 4s

(Yasukawa et al., 2010)

metathoracic leg tarsal taste bristle 5b

(Yasukawa et al., 2010)

metathoracic leg tarsal taste bristle 4s

(Yasukawa et al., 2010)

adult stage | female

prothoracic leg tarsal taste bristle 4s

(Yasukawa et al., 2010)

prothoracic leg tarsal taste bristle 4c

(Yasukawa et al., 2010)

prothoracic leg tarsal taste bristle 5b

(Yasukawa et al., 2010)

prothoracic leg tarsal taste bristle 5s

(Yasukawa et al., 2010)

mesothoracic leg tarsal taste bristle 5b

(Yasukawa et al., 2010)

mesothoracic leg tarsal taste bristle 4s

(Yasukawa et al., 2010)

metathoracic leg tarsal taste bristle 5b

(Yasukawa et al., 2010)

metathoracic leg tarsal taste bristle 4s

(Yasukawa et al., 2010)

Reporter: P{Obp57e-lacZ.G}

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

leg taste bristle

(Galindo and Smith, 2001)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Obp57e in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Obp57e

View exonic expression by tissue for Dmel\Obp57e

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

326110/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0050145

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0050145

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0050145

Flygut - An atlas of the Drosophila adult midgut

FBgn0050145

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 2 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Obp57e

Transgenic constructs containing regulatory region of Obp57e

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene disrupted in

Df(2R)Exel7163

(Ryder, 2004.4.26)

Df(2R)Obp57d-e^KO

(Matsuo et al., 2007)

Inferred from location ( 7 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Scer\GAL4^Mef2.PR

Obp57e^GD5012

viable, with Scer\GAL4^pnr-MD237

Obp57e^GD5012

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (0)

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (0)

Mus musculus (laboratory mouse) (0)

Xenopus tropicalis (Western clawed frog) (0)

Danio rerio (Zebrafish) (0)

Caenorhabditis elegans (Nematode, roundworm) (0)

Anopheles gambiae (African malaria mosquito) (20)

Agam\AgaP_AGAP012322

1 of 12

Yes

Agam\AgaP_AGAP012714

1 of 12

Yes

Agam\AgaP_AGAP013182

1 of 12

Yes

Agam\OBP2

1 of 12

Yes

Agam\OBP3

1 of 12

Yes

Agam\OBP4

1 of 12

Yes

Agam\OBP5

1 of 12

Yes

Agam\OBP6

1 of 12

Yes

Agam\OBP7

1 of 12

Yes

Agam\OBP9

1 of 12

Yes

Agam\OBP10

1 of 12

Yes

Agam\OBP17

1 of 12

Yes

Agam\OBP18

1 of 12

Yes

Agam\OBP19

1 of 12

Yes

Agam\OBP20

1 of 12

Yes

Agam\OBP21

1 of 12

Yes

Agam\OBP23

1 of 12

Yes

Agam\OBP25

1 of 12

Yes

Agam\OBP26

1 of 12

Yes

Agam\OBP28

1 of 12

Yes

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Obp57e. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (32)

4 of 13

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Other Interaction Browsers

Summary of Genetic Interactions

esyN Network Diagram

Other Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0050145

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-91

Cytogenetic map

57A5-57A5

Sequence location

2R:20,547,816..20,548,291 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

57A5-57A5

Limits computationally determined from genome sequence between P{EP}l(2)05510^EP2587&P{lacW}rig^k07917 and P{lacW}l(2)k02206^k02206

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-91

(FlyBase, 2016)

2-97.4

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (3)

Aberrations including deletions of this gene Aberrations including duplications of this gene

NIG-Fly

HMJ24092

y¹ v¹; P{TRiP.HMJ24092}attP40/CyO

2RG-1321

y² VhaAC39-1^cho-1 v¹; P{U6-Obp57e.NIG.sgRNA}attP40/CyO

VDRC

v105001

P{KK112540}VIE-260B

Genomic Clones (17)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (1)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

RT04042

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0050145

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0050145

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Additional comments

Annotation CG13429 split into CG30145 (Obp57e) and CG30148 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

FlyBase curator comment: The gene referred to as "Obp57d" in FBrf0151704 is not the same gene as the one referred to as "Obp57d" in FlyBase (FBgn0043536). The gene referred to as "Obp57d" in FBrf0151704 actually corresponds to FBgn0034516 which is referred to in FlyBase with the symbol "Obp57e" (and corresponds to release 3 annotation CG30145).

(Hekmat-Scafe et al., 2002)

FlyBase curator comment: The gene referred to as "Obp57d" in FBrf0151651 is not the same gene as the one referred to as "Obp57d" in FlyBase (FBgn0043536). The gene referred to as "Obp57d" in FBrf0151651 actually corresponds to FBgn0034516 which is referred to in FlyBase with the symbol "Obp57e" (and corresponds to release 3 annotation CG30145).

(Hekmat-Scafe, 2002.3.5)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

57e

(Graham and Davies, 2002, Smith, 2002.1.14)

CG13429

(Graham, 2002.6.18, Hekmat-Scafe, 2002.3.5, Hekmat-Scafe et al., 2002, Vogt et al., 2002, Robertson, 2000.8.17)

CG30145

(National Institute of Genetics Fly Stocks, 2023-, National Institute of Genetics Fly Stocks, 2022-, Chen et al., 2012, Zhang et al., 2010, Keleman et al., 2009.8.5)

DmelObp57e

(Vieira and Rozas, 2011, Sánchez-Gracia et al., 2009)

Dmel\Obp57e

(Tomioka et al., 2012, Yasukawa et al., 2010)

OBP57e

(Rihani et al., 2021, Galindo and Smith, 2001)

Obp57d

(Hekmat-Scafe, 2002.3.5, Hekmat-Scafe et al., 2002)

Obp57e

(Anholt, 2020, Anholt et al., 2020, He et al., 2019, Markow, 2019, Mohapatra and Menuz, 2019, Xu and Wang, 2016, Librado and Rozas, 2013, Yamamoto and Ishikawa, 2013, Koganezawa et al., 2010, Yasukawa et al., 2010, Zhou et al., 2009, Harada et al., 2008, Huang et al., 2008, Matsuo, 2008, Matsuo et al., 2007, Vieira et al., 2007)

Name Synonyms

Odorant-binding protein 57e

(Matsuo et al., 2007, Galindo and Smith, 2001)

Secondary FlyBase IDs

FBgn0034516

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 22 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

326110

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

AAF57460

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9V938

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9V938

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9V938

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

326110/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0050145

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0050145

FlyMine - An integrated database for Drosophila genomics

FBgn0050145

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG30145

MARRVEL_MODEL - MARRVEL (model organism gene)

326110

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0050145

DroID - A comprehensive database of gene and protein interactions.

FBgn0050145

DRSC - Results frm RNAi screens

FBgn0050145

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Obp57e_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0050145

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0050145

Flygut - An atlas of the Drosophila adult midgut

FBgn0050145

References (51)

The following external sites may use different assemblies or annotations than FlyBase.

NA

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