FlyBase Gene Report: Dmel\CG30158

General Information

Symbol

Dmel\CG30158

Species

D. melanogaster

Name

Annotation Symbol

CG30158

Feature Type

protein_coding_gene

FlyBase ID

FBgn0050158

Gene Model Status

Current

Stock Availability

38 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

42D6-42E1

Sequence location

2R:6,891,760..6,922,613 [+]

Recombination map

2-55

RefSeq locus

NT_033778 REGION:6891760..6922613

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (4 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables GDP binding

inferred from biological aspect of ancestor with PANTHER:PTN000631348

(GO Reference Genome Project, 2011-)

enables GTP binding

inferred from biological aspect of ancestor with PANTHER:PTN000631348

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR001806, InterPro:IPR005225

(InterPro Project Members, 2004-)

enables GTPase activity

inferred from biological aspect of ancestor with PANTHER:PTN000631348

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR001806

(InterPro Project Members, 2004-)

Biological Process (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN000631348

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

RAS GTPASES

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

GTPase activity
RHEA 19669:

Summaries

Gene Group (FlyBase)

RAS GTPASES -: The Ras family are members of the Ras superfamily of small GTPases. Ras proteins act in signal transduction cascades, generally the activated GTPase recruits and activates downstream effectors. Members this family are typically membrane-associated via isoprenylation of a CaaX motif in the C-terminus. (Adapted from PMID:15731001).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\CG30158 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry A1Z6R7)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.48

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

CG30158-RA

FBtr0086165

NM_144058

3657

280

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

CG30158-PA

FBpp0085498

30.2

280

9.73

A1Z6R7

NP_652315

AAF57410

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CG30158 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG30158_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.72

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\CG30158 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\CG30158

View exonic expression by tissue for Dmel\CG30158

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0050158

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

50149/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0050158

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0050158

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0050158

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG30158

Flygut - An atlas of the Drosophila adult midgut

FBgn0050158

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 9 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of CG30158

Transgenic constructs containing regulatory region of CG30158

Aberrations (Deficiencies and Duplications) ( 3 )

Inferred from experimentation ( 3 )

Gene disrupted in

Df(2R)Δ42E

(Lin et al., 2005)

Df(2R)Exel6051

(Ryder, 2004.4.26)

Gene partially disrupted in

Dp(2;2)SM5

(Miller et al., 2018)

Inferred from location ( 8 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

CG30158^GD8669

viable, with Scer\GAL4^Mef2.PR

CG30158^GD8669

viable, with Scer\GAL4^pnr-MD237

CG30158^GD8669

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (58)

Hsap\RASD1

3 of 14

Yes

Hsap\RASD2

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (35)

Rnor\Rasd1

3 of 14

Yes

Rnor\Rasd2

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (38)

Mmus\Rasd1

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (35)

Xtro\rasd1

2 of 13

Yes

Xtro\rasd2

2 of 13

Yes

Xtro\cdc42

1 of 13

Xtro\col11a2

1 of 13

Xtro\comt.2

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (44)

Drer\rasd3

5 of 14

Yes

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Drer\si:dkeyp-59c12.1

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (33)

Cele\ssr-2

9 of 14

Yes

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Cele\D1081.12

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Anopheles gambiae (African malaria mosquito) (29)

Agam\AgaP_AGAP005248

12 of 12

Yes

3 of 12

3 of 12

3 of 12

3 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (11)

Atha\RA-5

1 of 13

Yes

Atha\RAB6A

1 of 13

Yes

Atha\RAB7A

1 of 13

Yes

Atha\RAB7B

1 of 13

Yes

Atha\RABG1

1 of 13

Yes

Atha\RABG3B

1 of 13

Yes

Atha\RABG3d

1 of 13

Yes

Atha\RABG3E

1 of 13

Yes

Atha\RABH1c

1 of 13

Yes

Atha\RABH1d

1 of 13

Yes

Atha\RABH1e

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (7)

Scer\RAS1

2 of 13

Yes

Scer\RAS2

2 of 13

Yes

Scer\RHB1

2 of 13

Yes

Scer\RSR1

2 of 13

Yes

Scer\CDC42

1 of 13

Scer\YPT6

1 of 13

Scer\YPT53

1 of 13

Schizosaccharomyces pombe (Fission yeast) (3)

Spom\ras1

2 of 12

Yes

Spom\rhb1

2 of 12

Yes

Spom\ypt1

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:CG30158. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (29)

4 of 13

4 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

Ric

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

3 of 14

605550

RASD2; RASD family member 2

3 of 14

612842

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Other Interaction Browsers

Summary of Genetic Interactions

esyN Network Diagram

Other Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

72565

DroID - A comprehensive database of gene and protein interactions.

FBgn0050158

MIST (protein-protein) - An integrated Molecular Interaction Database

50149

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-55

Cytogenetic map

42D6-42E1

Sequence location

2R:6,891,760..6,922,613 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

42D6-42E1

Limits computationally determined from genome sequence between P{lacW}ubl^k03203&P{PZ}CG3287⁰⁹⁹⁶⁷ and P{EP}EP2303&P{EP}EP2105

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-55

(FlyBase, 2016)

2-56.1

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (38)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

71194

w¹¹¹⁸; P{VT012760-p65.AD}attP40

Korea

12866

P{VT012761-GAL4}attP2

12867

P{VT012764-GAL4}attP2

12868

P{VT012768-GAL4}attP2

15050

P{VT012759-GAL4}attP2

11668

P{EP}CG30158^G17937

14108

P{VT012761-lexA::GADfl}attP40

14109

P{VT012765-lexA::GADfl}attP40

16405

P{VT012759-lexA::GADfl}attP40

VDRC

v104798

P{KK109540}VIE-260B

v200022

P{VT012760-GAL4}attP2

v32434

w¹¹¹⁸; P{GD8669}v32434

More stocks available...

Genomic Clones (45)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (7)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE28276

Other clones

FI21445

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0050158

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0050158

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Source for merge of: CG30158 BcDNA:RE28276

(FlyBase, 1996-)

Source for merge of: CG15243 CG15244 CG9464

(FlyBase Genome Annotators, 2002-2003)

Additional comments

Annotations CG15243, CG15244 and CG9464 merged as CG30158 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Source for merge of CG30158 BcDNA:RE28276 was a shared cDNA ( date:030728 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

BcDNA:RE28276

CG15243

CG15244

CG30158

(National Institute of Genetics Fly Stocks, 2022-, Gene Disruption Project members, 2018-, Miller et al., 2018, Clandinin and Owens, 2016-, Diez et al., 2011, Kong et al., 2010, Garrett et al., 2009, Keleman et al., 2009.8.5, Ni et al., 2008, Bakal et al., 2007)

CG9464

Name Synonyms

Secondary FlyBase IDs

FBgn0033116
FBgn0040675
FBgn0040676
FBgn0063683

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 36 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

50149

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

A1Z6R7

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

A1Z6R7

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0050158

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

50149/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0050158

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0050158

FlyMine - An integrated database for Drosophila genomics

FBgn0050158

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG30158

MARRVEL_MODEL - MARRVEL (model organism gene)

50149

Linkouts

BioGRID - A database of protein and genetic interactions.

72565

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0050158

DroID - A comprehensive database of gene and protein interactions.

FBgn0050158

DRSC - Results frm RNAi screens

FBgn0050158

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG30158_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0050158

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0050158

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG30158

Flygut - An atlas of the Drosophila adult midgut

FBgn0050158

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC003668

MIST (protein-protein) - An integrated Molecular Interaction Database

50149

References (35)

The following external sites may use different assemblies or annotations than FlyBase.

-0.72

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