FlyBase Gene Report: Dmel\CG30158

RAS GTPASES -: The Ras family are members of the Ras superfamily of small GTPases. Ras proteins act in signal transduction cascades, generally the activated GTPase recruits and activates downstream effectors. Members this family are typically membrane-associated via isoprenylation of a CaaX motif in the C-terminus. (Adapted from PMID:15731001).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\CG30158 or the JBrowse view of Dmel\CG30158 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.48

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

CG30158-RA

FBtr0086165

NM_144058

3657

280

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

CG30158-PA

FBpp0085498

30.2

280

9.73

NP_652315

AAF57410

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CG30158 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG30158_1

Linkouts

Gene Ontology (5 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

GDP binding

inferred from biological aspect of ancestor with PANTHER:PTN000631348

(assigned by GO_Central )

(Gaudet et al., 2011)

GTP binding

inferred from biological aspect of ancestor with PANTHER:PTN000631348

(assigned by GO_Central )

(Gaudet et al., 2011)

GTPase activity

inferred from biological aspect of ancestor with PANTHER:PTN000631348

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

signal transduction

inferred from electronic annotation with InterPro:IPR020849

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN000631348

(assigned by GO_Central )

(Gaudet et al., 2011)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\CG30158 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\CG30158

View exonic expression by tissue for Dmel\CG30158

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0050158

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0050158

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG30158-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG30158

Flygut - An atlas of the Drosophila adult midgut

FBgn0050158

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 7 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of CG30158

Transgenic constructs containing regulatory region of CG30158

Deletions and Duplications ( 2 )

Disrupted in

Df(2R)Δ42E

(Lin et al., 2005)

Df(2R)Exel6051

(Ryder, 2004.4.26)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

CG30158^GD8669

viable, with Scer\GAL4^Mef2.PR

CG30158^GD8669

viable, with Scer\GAL4^pnr-MD237

CG30158^GD8669

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (7)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\RASD1

3 of 15

Yes

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (5)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Rasd1

3 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

Rattus norvegicus (Norway rat) (5)

Rnor\Rasd1

2 of 13

Yes

Rnor\Rasd2

2 of 13

Yes

Rnor\Diras1

1 of 13

Rnor\Diras2

1 of 13

Rnor\Rhebl1

1 of 13

Xenopus tropicalis (Western clawed frog) (7)

Xtro\LOC733456

2 of 12

Yes

Xtro\ras-dva

2 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Danio rerio (Zebrafish) (8)

Drer\rasd4

5 of 15

Yes

4 of 15

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (4)

Cele\ssr-2

9 of 15

Yes

Cele\D1081.4

1 of 15

Yes

Cele\drn-1

1 of 15

Cele\rheb-1

1 of 15

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (2)

Scer\RHB1

1 of 15

Yes

Scer\RSR1

1 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\rhb1

1 of 12

Yes

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190EXU )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Dmel\CG30158

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA15685

Drosophila persimilis

Dper\GL11127

Drosophila willistoni

Dwil\GK20887

Drosophila virilis

Dvir\GJ17556

Drosophila mojavensis

Dmoj\GI19720

Drosophila grimshawi

Dgri\GH22014

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150DIF )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17019081

Glossina morsitans

Tsetse fly

Gmor\GMOY002445

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_09060

Mayetiola destructor

Hessian fly

Mdes\Mdes011148

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL007766

Anopheles darlingi

American malaria mosquito

Adar\ADAC010659

Anopheles gambiae

Malaria mosquito

Agam\AGAP005248

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ018718

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0GEC )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis florea

Little honeybee

Aflo\2241033716

Apis mellifera

Western honey bee

Amel\GB53387

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP10379

Bombus terrestris

Buff-tailed bumblebee

Bter\15207457

Linepithema humile

Argentine ant

Lhum\LH20218

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:002801

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG002305

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_11105

Tribolium castaneum

Red flour beetle

Tcas\TC003668

Pediculus humanus

Human body louse

Phum\PHUM192620

Rhodnius prolixus

Kissing bug

Rpro\RPRC003248

Cimex lectularius

Bed bug

Clec\CLEC012628

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI002901

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_05948

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0GEW )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR006211

Strigamia maritima

European centipede

Smar\SMAR013067

Ixodes scapularis

Black-legged tick

Isca\ISCW003069

Stegodyphus mimosarum

African social velvet spider

Smim\KFM74399.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM65329.1

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0U2W )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-RasD1/2L2

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-RasDL

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-RasD1/2

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-RasD1/2L1

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Ras/Dva

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-RasD

Gallus gallus

Domestic chicken

Ggal\RASD2

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000021552

Gallus gallus

Domestic chicken

Ggal\RASD1

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (11)

Gene Symbol

Score

Source

Alignment

6 of 10

4 of 10

3 of 10

2 of 10

2 of 10

2 of 10

Ric

2 of 10

1 of 10

1 of 10

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

3 of 15

605550

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

72565

DroID - A comprehensive database of gene and protein interactions.

FBgn0050158

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

50149

MIST (protein-protein) - An integrated Molecular Interaction Database

CG30158

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-55

Cytogenetic map

42D6-42E1

Sequence location

2R:6,891,760..6,922,613 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

42D6-42E1

Limits computationally determined from genome sequence between P{lacW}ubl^k03203&P{PZ}CG3287⁰⁹⁹⁶⁷ and P{EP}EP2303&P{EP}EP2105

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-55

(FlyBase, 2016)

2-56.1

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (7)

Bloomington

31162

y¹ v¹; P{TRiP.JF01655}attP2

24291

w¹¹¹⁸; Mi{ET1}CG30158^MB04149

19014

w¹¹¹⁸; PBac{WH}CG30158^f06824/CyO

64744

w¹¹¹⁸; PBac{IT.GAL4}CG30158^0798-G4

Kyoto

103889

y^* w^*; P{GawB}CG30158^NP1113 / CyO, P{UAS-lacZ.UW14}UW14

VDRC

v104798

P{KK109540}VIE-260B

v32434

w¹¹¹⁸; P{GD8669}v32434

Genomic Clones (45)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (11)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

RE28276

Other clones

FI21445

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0050158

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RE05206

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0050158

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

50149

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: CG30158 BcDNA:RE28276

(FlyBase, 1996-)

Source for merge of: CG15243 CG15244 CG9464

(FlyBase Genome Annotators, 2002-2003)

Additional comments

Annotations CG15243, CG15244 and CG9464 merged as CG30158 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Source for merge of CG30158 BcDNA:RE28276 was a shared cDNA ( date:030728 ).

(FlyBase, 1996-)

Other Comments

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 34 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

50149

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0050158

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0050158

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG30158_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG30158

Flygut - An atlas of the Drosophila adult midgut

FBgn0050158

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

50149

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC003668

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG30158

modMine - A data warehouse for the modENCODE project

FBgn0050158

Linkouts

BioGRID - A database of protein and genetic interactions.

72565

DroID - A comprehensive database of gene and protein interactions.

FBgn0050158

DRSC - Results frm RNAi screens

FBgn0050158

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0050158

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG30158-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0050158

FlyMine - An integrated database for Drosophila genomics

FBgn0050158

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

50149

MIST (protein-protein) - An integrated Molecular Interaction Database

CG30158

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

BcDNA:RE28276

CG15243

CG15244

CG30158

(Gene Disruption Project members, 2018-, Miller et al., 2018, Clandinin and Owens, 2016-, Diez et al., 2011, Kong et al., 2010, Garrett et al., 2009, Keleman et al., 2009.8.5, Ni et al., 2008, Bakal et al., 2007)

CG9464

Name Synonyms

Secondary FlyBase IDs

FBgn0033116
FBgn0040675
FBgn0040676
FBgn0063683

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (31)

Report Sections

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