FlyBase Gene Report: Dmel\AIMP3

General Information

Symbol

Dmel\AIMP3

Species

D. melanogaster

Name

aaRS-interacting multifunctional protein 3

Annotation Symbol

CG30185

Feature Type

protein_coding_gene

FlyBase ID

FBgn0050185

Gene Model Status

Current

Stock Availability

3 publicly available

Gene Summary

aaRS-interacting multifunctional protein 3 (AIMP3) is predicted to encode one of 3 non-enzymatic factors that combine with eight aminoacyl-tRNA synthetases to form a 'multi-synthetase complex' (MSC). The functional significance of the MSC is unclear, but the non-enzymatic factors are thought to be responsive to diverse signal transduction pathways. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

59E3-59E3

Sequence location

2R:23,544,024..23,544,901 [+]

Recombination map

2-103

RefSeq locus

NT_033778 REGION:23544024..23544901

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (4 terms)

Molecular Function (0 terms)

There is no information supporting a Molecular Function term for this gene. (Based on a review of available data on: 2021-01-28 )

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in positive regulation of DNA damage response, signal transduction by p53 class mediator

inferred from sequence or structural similarity with UniProtKB:Q9D1M4

(FlyBase Curators, 2008-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

part_of aminoacyl-tRNA synthetase multienzyme complex

inferred from sequence or structural similarity with UniProtKB:Q9D1M4

(FlyBase Curators, 2008-)

inferred from biological aspect of ancestor with PANTHER:PTN002549087

(assigned by GO_Central )

(Gaudet et al., 2011)

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN002549087

(assigned by GO_Central )

(Gaudet et al., 2011)

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN002549087

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\AIMP3 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q8MKK1)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.52

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

AIMP3-RA

FBtr0072060

NM_145193

739

179

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

AIMP3-PA

FBpp0071969

20.0

179

7.05

NP_660194

AAM68253

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\AIMP3 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG30185_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\AIMP3 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\AIMP3

View exonic expression by tissue for Dmel\AIMP3

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0050185

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

246507/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0050185

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0050185

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0050185

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG30185

Flygut - An atlas of the Drosophila adult midgut

FBgn0050185

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 4 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of AIMP3

Transgenic constructs containing regulatory region of AIMP3

Aberrations (Deficiencies and Duplications) ( 7 )

Inferred from experimentation ( 7 )

Gene disrupted in

Df(2R)bw5

(Marygold et al., 2007)

Df(2R)bw-HB132

(Marygold et al., 2007)

Df(2R)bw-S46

(Marygold et al., 2007)

Df(2R)bw-WI3121

(Marygold et al., 2007)

Df(2R)Exel7179

(Ryder, 2004.4.26)

Df(2R)Exel7180

(Marygold et al., 2007)

Df(2R)or-BR6

(Marygold et al., 2007)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

Sterility

Allele

fertile

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (7)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\EEF1E1

10 of 15

Yes

Hsap\EEF1E1-BLOC1S5

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (9)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Eef1e1

9 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Rattus norvegicus (Norway rat) (8)

Rnor\Eef1e1

9 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Xenopus tropicalis (Western clawed frog) (5)

Xtro\eef1e1

6 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

Danio rerio (Zebrafish) (7)

Drer\eef1e1

9 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (4)

Cele\eef-1G

1 of 15

Yes

Cele\gst-42

1 of 15

Yes

Cele\gst-43

1 of 15

Yes

Cele\Y53G8B.1

1 of 15

Yes

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (6)

Scer\CAM1

1 of 15

Yes

Scer\GTT1

1 of 15

Yes

Scer\GTT2

1 of 15

Yes

Scer\TEF4

1 of 15

Yes

Scer\URE2

1 of 15

Yes

Scer\YGR201C

1 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (34)

Gene Symbol

Score

Source

Alignment

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

EEF1E1; eukaryotic translation elongation factor 1 epsilon 1

10 of 15

609206

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

AIMP3 - MetRS

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0050185

MIST (protein-protein) - An integrated Molecular Interaction Database

AIMP3

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-103

Cytogenetic map

59E3-59E3

Sequence location

2R:23,544,024..23,544,901 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

59E3-59E3

Limits computationally determined from genome sequence between P{lacW}l(2)s4830^s4830&P{lacW}chrw^k06908 and P{PZ}Dcp-1⁰²¹³²

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-103

(FlyBase, 2016)

2-108.4

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (3)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

19348

w¹¹¹⁸; P{XP}AIMP3^d11316

Kyoto

124217

w¹¹¹⁸; P{RS3}AIMP3^CB-6776-3

VDRC

v104364

P{KK107934}VIE-260B

Genomic Clones (15)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (18)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0050185

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0050185

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

246507

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: AIMP3 CG30185

(Lu et al., 2015)

Source for database merge of

Source for merge of: CG30185 anon- EST:Posey25

(Ashburner, 2004.6.30)

Additional comments

Annotation CG5365 split into CG33150 (Gr59f) and CG30185 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Other Comments

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 40 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

246507

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q8MKK1

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q8MKK1

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0050185

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0050185

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

246507/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0050185

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG30185_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0050185

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0050185

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG30185

Flygut - An atlas of the Drosophila adult midgut

FBgn0050185

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

246507

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG30185

MARRVEL_MODEL - MARRVEL (model organism gene)

246507

modMine - A data warehouse for the modENCODE project

FBgn0050185

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0050185

DRSC - Results frm RNAi screens

FBgn0050185

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0050185

FlyMine - An integrated database for Drosophila genomics

FBgn0050185

MIST (protein-protein) - An integrated Molecular Interaction Database

AIMP3

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

AIMP3

(Lu et al., 2015, Bischof and FlyORF project members, 2014.6.20)

CG30185

(Kolkhof et al., 2017, Çiçek et al., 2016, Lu et al., 2015, Kwon et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Keleman et al., 2009.8.5, Fisher et al., 2008, Hughes et al., 2008, Marygold et al., 2007, Dworkin and Gibson., 2006)

CG5365

(Dunipace et al., 2001, BDGP Project Members, 2000-)

anon-EST:Posey25

Name Synonyms

aaRS-interacting multifunctional protein 3

(Lu et al., 2015)

aminoacyl-tRNA synthetase-interacting multifunctional protein 3

(Lu et al., 2015)

Secondary FlyBase IDs