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Gene Dmel\CG30344

General Information
Symbol	Dmel\CG30344	Species	D. melanogaster
Name		Annotation symbol	CG30344
Feature type	protein_coding_gene	FlyBase ID	FBgn0050344
Gene Model Status	Current	Stock availability	3 publicly available
Also Known As	CG8054, MET
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	45A12-45A13	Sequence location	2R:5,032,765..5,036,017 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	This gene is referred to in FlyBase by the symbol Dmel\CG30344 (FBgn0050344). It is a protein_coding_gene from Drosophila melanogaster. An electronic pipeline based on InterPro domains suggests that it has the molecular function: transporter activity. There is experimental evidence that it is involved in the biological process: response to methotrexate. 2 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Major facilitator superfamily; Major facilitator superfamily domain; Major facilitator superfamily domain, general substrate transporter; Sugar transporter, conserved site. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed during late larval stages, during early pupal stages. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult organs/tissues expressed at moderate or high levels. Expression at high levels in the following post-embryonic organs or tissues: larval midgut, larval/adult Malpighian tubules, adult heart, inseminated spermatheca, adult male accessory gland. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult crop, adult midgut, larval/adult hindgut, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1631387_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of CG30344. Gene sequence location is 2R:5032765..5036017.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 45A12-45A13   Limits computationally determined from genome sequence between P{lacW}CG8788k04512&P{lacW}l(2)k13412k13412 and P{PZ}Pkn06736
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\CG30344 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Low-frequency RNA-Seq exon junction(s) not annotated. Gene model reviewed during 5.49
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) CG30344-RA FBtr0088583  NM_165655   2147   507
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein CG30344-PA   FBpp0087664   55.9   507   5.85     NP_724759   AAM68813
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Sugar transporter, conserved site (IPR005829) Major facilitator superfamily (IPR011701) Major facilitator superfamily domain, general substrate transporter (IPR016196) Major facilitator superfamily domain (IPR020846)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA142295   AE013599 AAM68813   AI292950   AI403908   AW940906   AY051590 AAK93014   BG637383   BI163853   BI164719   BI212793   BI231588   BI242146   BI371089   BI371146   BI565842   BI613954   BI616931   CA803210   CA806402   CA807059   CA807112   CO285210   CO299497   CO309007   CO325852   CO326196   EC066323   EC211045   EC211209   EC256809   EL882407   EL887339   EV576819
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q961H2
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 9 -- 10   amnioserosa   (Fisher et al., 2012) head mesoderm   (Fisher et al., 2012) embryonic stage 11 -- 12   amnioserosa   (Fisher et al., 2012) garland cell primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic/larval garland cell   (Fisher et al., 2012) yolk nucleus   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 7 of adulthood -- day 49 of adulthood   adult heart   (Cammarato et al., 2011)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult organs/tissues expressed at moderate or high levels. Expression at high levels in the following post-embryonic organs or tissues: larval midgut, larval/adult Malpighian tubules, adult heart, inseminated spermatheca, adult male accessory gland. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult crop, adult midgut, larval/adult hindgut, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    67.85 Larval Midgut    836 Larval Hindgut    201.9 Larval Malpighian Tubules    1228.9 Larval Fat Body    211.6 Larval Salivary Gland    181.8 Larval Trachea    114.975 Larval Carcass    126 Adult Head    164.7 Adult Eye    172.075 Adult Brain    92.4 Adult Thoracic-Abdominal Ganglion    91.5 Adult Crop    222.8 Adult Midgut    184.5 Adult Hindgut    241.8 Adult Malpighian Tubules    1522.8 Adult Fat Body    158.1 Adult Salivary Gland    200.2 Adult Heart    715.525 Adult VirginFemale Spermatheca    395.5 Adult InseminatedFemale Spermatheca    544.2 Adult Ovary    115.5 Adult Testis    36.3 Adult Male Accessory Gland    1191.4 Adult Carcass    151.6 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    67.85 Larval Midgut  (836) Larval Hindgut    201.9 Larval Malpighian Tubules  (1228.9) Larval Fat Body    211.6 Larval Salivary Gland    181.8 Larval Trachea    114.975 Larval Carcass    126 Adult Head    164.7 Adult Eye    172.075 Adult Brain    92.4 Adult Thoracic-Abdominal Ganglion    91.5 Adult Crop    222.8 Adult Midgut    184.5 Adult Hindgut    241.8 Adult Malpighian Tubules  (1522.8) Adult Fat Body    158.1 Adult Salivary Gland    200.2 Adult Heart  (715.525) Adult VirginFemale Spermatheca    395.5 Adult InseminatedFemale Spermatheca  (544.2) Adult Ovary    115.5 Adult Testis    36.3 Adult Male Accessory Gland  (1191.4) Adult Carcass    151.6 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    67.85 Larval Midgut    836 Larval Hindgut    201.9 Larval Malpighian Tubules  (1228.9) Larval Fat Body    211.6 Larval Salivary Gland    181.8 Larval Trachea    114.975 Larval Carcass    126 Adult Head    164.7 Adult Eye    172.075 Adult Brain    92.4 Adult Thoracic-Abdominal Ganglion    91.5 Adult Crop    222.8 Adult Midgut    184.5 Adult Hindgut    241.8 Adult Malpighian Tubules  (1522.8) Adult Fat Body    158.1 Adult Salivary Gland    200.2 Adult Heart    715.525 Adult VirginFemale Spermatheca    395.5 Adult InseminatedFemale Spermatheca    544.2 Adult Ovary    115.5 Adult Testis    36.3 Adult Male Accessory Gland  (1191.4) Adult Carcass    151.6 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    67.85 Larval Midgut    836 Larval Hindgut    201.9 Larval Malpighian Tubules    1228.9 Larval Fat Body    211.6 Larval Salivary Gland    181.8 Larval Trachea    114.975 Larval Carcass    126 Adult Head    164.7 Adult Eye    172.075 Adult Brain    92.4 Adult Thoracic-Abdominal Ganglion    91.5 Adult Crop    222.8 Adult Midgut    184.5 Adult Hindgut    241.8 Adult Malpighian Tubules    1522.8 Adult Fat Body    158.1 Adult Salivary Gland    200.2 Adult Heart    715.525 Adult VirginFemale Spermatheca    395.5 Adult InseminatedFemale Spermatheca    544.2 Adult Ovary    115.5 Adult Testis    36.3 Adult Male Accessory Gland    1191.4 Adult Carcass    151.6 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    67.85 Larval Midgut    836 Larval Hindgut    201.9 Larval Malpighian Tubules    1228.9 Larval Fat Body    211.6 Larval Salivary Gland    181.8 Larval Trachea    114.975 Larval Carcass    126 Adult Head    164.7 Adult Eye    172.075 Adult Brain    92.4 Adult Thoracic-Abdominal Ganglion    91.5 Adult Crop    222.8 Adult Midgut    184.5 Adult Hindgut    241.8 Adult Malpighian Tubules    1522.8 Adult Fat Body    158.1 Adult Salivary Gland    200.2 Adult Heart    715.525 Adult VirginFemale Spermatheca    395.5 Adult InseminatedFemale Spermatheca    544.2 Adult Ovary    115.5 Adult Testis    36.3 Adult Male Accessory Gland    1191.4 Adult Carcass    151.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    67.85 Larval Midgut  (836) Larval Hindgut    201.9 Larval Malpighian Tubules  (1228.9) Larval Fat Body    211.6 Larval Salivary Gland    181.8 Larval Trachea    114.975 Larval Carcass    126 Adult Head    164.7 Adult Eye    172.075 Adult Brain    92.4 Adult Thoracic-Abdominal Ganglion    91.5 Adult Crop    222.8 Adult Midgut    184.5 Adult Hindgut    241.8 Adult Malpighian Tubules  (1522.8) Adult Fat Body    158.1 Adult Salivary Gland    200.2 Adult Heart  715.525 Adult VirginFemale Spermatheca    395.5 Adult InseminatedFemale Spermatheca  544.2 Adult Ovary    115.5 Adult Testis    36.3 Adult Male Accessory Gland  (1191.4) Adult Carcass    151.6 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    67.85 Larval Midgut    836 Larval Hindgut    201.9 Larval Malpighian Tubules  1228.9 Larval Fat Body    211.6 Larval Salivary Gland    181.8 Larval Trachea    114.975 Larval Carcass    126 Adult Head    164.7 Adult Eye    172.075 Adult Brain    92.4 Adult Thoracic-Abdominal Ganglion    91.5 Adult Crop    222.8 Adult Midgut    184.5 Adult Hindgut    241.8 Adult Malpighian Tubules  1522.8 Adult Fat Body    158.1 Adult Salivary Gland    200.2 Adult Heart    715.525 Adult VirginFemale Spermatheca    395.5 Adult InseminatedFemale Spermatheca    544.2 Adult Ovary    115.5 Adult Testis    36.3 Adult Male Accessory Gland  1191.4 Adult Carcass    151.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    67.85 Larval Midgut    836 Larval Hindgut    201.9 Larval Malpighian Tubules    1228.9 Larval Fat Body    211.6 Larval Salivary Gland    181.8 Larval Trachea    114.975 Larval Carcass    126 Adult Head    164.7 Adult Eye    172.075 Adult Brain    92.4 Adult Thoracic-Abdominal Ganglion    91.5 Adult Crop    222.8 Adult Midgut    184.5 Adult Hindgut    241.8 Adult Malpighian Tubules    1522.8 Adult Fat Body    158.1 Adult Salivary Gland    200.2 Adult Heart    715.525 Adult VirginFemale Spermatheca    395.5 Adult InseminatedFemale Spermatheca    544.2 Adult Ovary    115.5 Adult Testis    36.3 Adult Male Accessory Gland    1191.4 Adult Carcass    151.6 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    164.7   NA  Eye    172.075   NA  Brain    92.4   67.85  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    91.5   NA  Crop    222.8   836  Midgut    184.5   201.9  Hindgut    241.8   1228.9  Malpighian Tubules    1522.8   211.6  Fat Body    158.1   181.8  Salivary Gland    200.2   NA  Heart    715.525   114.975  Trachea    NA   NA  VirginFemale Spermatheca    395.5   NA  InseminatedFemale Spermatheca    544.2   NA  Ovary    115.5   NA  Testis    36.3   NA  Male Accessory Gland    1191.4   126  Carcass    151.6 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    5 central nervous system, larvae L3    5 central nervous system, pupae P8    3 head, virgin 1-day female    7 head, virgin 4-day female    6 head, virgin 20-day female    8 head, mated 1-day female    5 head, mated 4-day female    6 head, mated 20-day female    6 head, mated 1-day male    8 head, mated 4-day male    7 head, mated 20-day male    10 salivary gland, larvae L3 wandering    61 salivary gland, white prepupae    229 digestive system, larvae L3 wandering    74 digestive system, 1-day adult    14 digestive system, 4-day adult    8 digestive system, 20-day adult    12 fat body, larvae L3 wandering    2 fat body, white prepupae    6 fat body, pupae P8    4 carcass, larvae L3 wandering    7 carcass, 1-day adult    7 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    10 ovary, mated 4-day female    7 testis, mated 4-day male    6 accessory gland, mated 4-day male    38 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    5 central nervous system, larvae L3    5 central nervous system, pupae P8    3 head, virgin 1-day female    7 head, virgin 4-day female    6 head, virgin 20-day female    8 head, mated 1-day female    5 head, mated 4-day female    6 head, mated 20-day female    6 head, mated 1-day male    8 head, mated 4-day male    7 head, mated 20-day male    10 salivary gland, larvae L3 wandering  (61) salivary gland, white prepupae  (229) digestive system, larvae L3 wandering  (74) digestive system, 1-day adult    14 digestive system, 4-day adult    8 digestive system, 20-day adult    12 fat body, larvae L3 wandering    2 fat body, white prepupae    6 fat body, pupae P8    4 carcass, larvae L3 wandering    7 carcass, 1-day adult    7 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    10 ovary, mated 4-day female    7 testis, mated 4-day male    6 accessory gland, mated 4-day male  (38) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    5 central nervous system, larvae L3    5 central nervous system, pupae P8    3 head, virgin 1-day female    7 head, virgin 4-day female    6 head, virgin 20-day female    8 head, mated 1-day female    5 head, mated 4-day female    6 head, mated 20-day female    6 head, mated 1-day male    8 head, mated 4-day male    7 head, mated 20-day male    10 salivary gland, larvae L3 wandering    61 salivary gland, white prepupae  (229) digestive system, larvae L3 wandering    74 digestive system, 1-day adult    14 digestive system, 4-day adult    8 digestive system, 20-day adult    12 fat body, larvae L3 wandering    2 fat body, white prepupae    6 fat body, pupae P8    4 carcass, larvae L3 wandering    7 carcass, 1-day adult    7 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    10 ovary, mated 4-day female    7 testis, mated 4-day male    6 accessory gland, mated 4-day male    38 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    5 central nervous system, larvae L3    5 central nervous system, pupae P8    3 head, virgin 1-day female    7 head, virgin 4-day female    6 head, virgin 20-day female    8 head, mated 1-day female    5 head, mated 4-day female    6 head, mated 20-day female    6 head, mated 1-day male    8 head, mated 4-day male    7 head, mated 20-day male    10 salivary gland, larvae L3 wandering    61 salivary gland, white prepupae    229 digestive system, larvae L3 wandering    74 digestive system, 1-day adult    14 digestive system, 4-day adult    8 digestive system, 20-day adult    12 fat body, larvae L3 wandering    2 fat body, white prepupae    6 fat body, pupae P8    4 carcass, larvae L3 wandering    7 carcass, 1-day adult    7 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    10 ovary, mated 4-day female    7 testis, mated 4-day male    6 accessory gland, mated 4-day male    38 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    5 central nervous system, larvae L3    5 central nervous system, pupae P8    3 head, virgin 1-day female    7 head, virgin 4-day female    6 head, virgin 20-day female    8 head, mated 1-day female    5 head, mated 4-day female    6 head, mated 20-day female    6 head, mated 1-day male    8 head, mated 4-day male    7 head, mated 20-day male    10 salivary gland, larvae L3 wandering    61 salivary gland, white prepupae    229 digestive system, larvae L3 wandering    74 digestive system, 1-day adult    14 digestive system, 4-day adult    8 digestive system, 20-day adult    12 fat body, larvae L3 wandering    2 fat body, white prepupae    6 fat body, pupae P8    4 carcass, larvae L3 wandering    7 carcass, 1-day adult    7 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    10 ovary, mated 4-day female    7 testis, mated 4-day male    6 accessory gland, mated 4-day male    38 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    5 central nervous system, larvae L3    5 central nervous system, pupae P8    3 head, virgin 1-day female    7 head, virgin 4-day female    6 head, virgin 20-day female    8 head, mated 1-day female    5 head, mated 4-day female    6 head, mated 20-day female    6 head, mated 1-day male    8 head, mated 4-day male    7 head, mated 20-day male    10 salivary gland, larvae L3 wandering  (61) salivary gland, white prepupae  (229) digestive system, larvae L3 wandering  (74) digestive system, 1-day adult    14 digestive system, 4-day adult    8 digestive system, 20-day adult    12 fat body, larvae L3 wandering    2 fat body, white prepupae    6 fat body, pupae P8    4 carcass, larvae L3 wandering    7 carcass, 1-day adult    7 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    10 ovary, mated 4-day female    7 testis, mated 4-day male    6 accessory gland, mated 4-day male  (38) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    5 central nervous system, larvae L3    5 central nervous system, pupae P8    3 head, virgin 1-day female    7 head, virgin 4-day female    6 head, virgin 20-day female    8 head, mated 1-day female    5 head, mated 4-day female    6 head, mated 20-day female    6 head, mated 1-day male    8 head, mated 4-day male    7 head, mated 20-day male    10 salivary gland, larvae L3 wandering    61 salivary gland, white prepupae  (229) digestive system, larvae L3 wandering    74 digestive system, 1-day adult    14 digestive system, 4-day adult    8 digestive system, 20-day adult    12 fat body, larvae L3 wandering    2 fat body, white prepupae    6 fat body, pupae P8    4 carcass, larvae L3 wandering    7 carcass, 1-day adult    7 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    10 ovary, mated 4-day female    7 testis, mated 4-day male    6 accessory gland, mated 4-day male    38 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    5 central nervous system, larvae L3    5 central nervous system, pupae P8    3 head, virgin 1-day female    7 head, virgin 4-day female    6 head, virgin 20-day female    8 head, mated 1-day female    5 head, mated 4-day female    6 head, mated 20-day female    6 head, mated 1-day male    8 head, mated 4-day male    7 head, mated 20-day male    10 salivary gland, larvae L3 wandering    61 salivary gland, white prepupae    229 digestive system, larvae L3 wandering    74 digestive system, 1-day adult    14 digestive system, 4-day adult    8 digestive system, 20-day adult    12 fat body, larvae L3 wandering    2 fat body, white prepupae    6 fat body, pupae P8    4 carcass, larvae L3 wandering    7 carcass, 1-day adult    7 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    10 ovary, mated 4-day female    7 testis, mated 4-day male    6 accessory gland, mated 4-day male    38 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed during late larval stages, during early pupal stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    27 embryo 04-06hr    25 embryo 06-08hr    27 embryo 08-10hr    33 embryo 10-12hr    49 embryo 12-14hr    51 embryo 14-16hr    52 embryo 16-18hr    48 embryo 18-20hr    25 embryo 20-22hr    11 embryo 22-24hr    12 larva L1    6 larva L2    12 larva L3 12hr old    11 larva L3 puffstage 1-2    30 larva L3 puffstage 3-6    49 larva L3 puffstage 7-9    85 white prepupae new    67 white prepupae 12hr    34 white prepupae 24hr    14 pupae 2d postWPP    15 pupae 3d postWPP    25 pupae 4d postWPP    18 adult male 01day    44 adult male 05day    33 adult male 30day    33 adult female 01day    36 adult female 05day    24 adult female 30day    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr  (27) embryo 04-06hr    25 embryo 06-08hr  (27) embryo 08-10hr  (33) embryo 10-12hr  (49) embryo 12-14hr  (51) embryo 14-16hr  (52) embryo 16-18hr  (48) embryo 18-20hr    25 embryo 20-22hr    11 embryo 22-24hr    12 larva L1    6 larva L2    12 larva L3 12hr old    11 larva L3 puffstage 1-2  (30) larva L3 puffstage 3-6  (49) larva L3 puffstage 7-9  (85) white prepupae new  (67) white prepupae 12hr  (34) white prepupae 24hr    14 pupae 2d postWPP    15 pupae 3d postWPP    25 pupae 4d postWPP    18 adult male 01day  (44) adult male 05day  (33) adult male 30day  (33) adult female 01day  (36) adult female 05day    24 adult female 30day    24 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    27 embryo 04-06hr    25 embryo 06-08hr    27 embryo 08-10hr    33 embryo 10-12hr    49 embryo 12-14hr    51 embryo 14-16hr    52 embryo 16-18hr    48 embryo 18-20hr    25 embryo 20-22hr    11 embryo 22-24hr    12 larva L1    6 larva L2    12 larva L3 12hr old    11 larva L3 puffstage 1-2    30 larva L3 puffstage 3-6    49 larva L3 puffstage 7-9    85 white prepupae new    67 white prepupae 12hr    34 white prepupae 24hr    14 pupae 2d postWPP    15 pupae 3d postWPP    25 pupae 4d postWPP    18 adult male 01day    44 adult male 05day    33 adult male 30day    33 adult female 01day    36 adult female 05day    24 adult female 30day    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    27 embryo 04-06hr    25 embryo 06-08hr    27 embryo 08-10hr    33 embryo 10-12hr    49 embryo 12-14hr    51 embryo 14-16hr    52 embryo 16-18hr    48 embryo 18-20hr    25 embryo 20-22hr    11 embryo 22-24hr    12 larva L1    6 larva L2    12 larva L3 12hr old    11 larva L3 puffstage 1-2    30 larva L3 puffstage 3-6    49 larva L3 puffstage 7-9    85 white prepupae new    67 white prepupae 12hr    34 white prepupae 24hr    14 pupae 2d postWPP    15 pupae 3d postWPP    25 pupae 4d postWPP    18 adult male 01day    44 adult male 05day    33 adult male 30day    33 adult female 01day    36 adult female 05day    24 adult female 30day    24 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    27 embryo 04-06hr    25 embryo 06-08hr    27 embryo 08-10hr    33 embryo 10-12hr    49 embryo 12-14hr    51 embryo 14-16hr    52 embryo 16-18hr    48 embryo 18-20hr    25 embryo 20-22hr    11 embryo 22-24hr    12 larva L1    6 larva L2    12 larva L3 12hr old    11 larva L3 puffstage 1-2    30 larva L3 puffstage 3-6    49 larva L3 puffstage 7-9    85 white prepupae new    67 white prepupae 12hr    34 white prepupae 24hr    14 pupae 2d postWPP    15 pupae 3d postWPP    25 pupae 4d postWPP    18 adult male 01day    44 adult male 05day    33 adult male 30day    33 adult female 01day    36 adult female 05day    24 adult female 30day    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr  27 embryo 04-06hr    25 embryo 06-08hr  27 embryo 08-10hr  (33) embryo 10-12hr  (49) embryo 12-14hr  (51) embryo 14-16hr  (52) embryo 16-18hr  (48) embryo 18-20hr    25 embryo 20-22hr    11 embryo 22-24hr    12 larva L1    6 larva L2    12 larva L3 12hr old    11 larva L3 puffstage 1-2  30 larva L3 puffstage 3-6  (49) larva L3 puffstage 7-9  (85) white prepupae new  (67) white prepupae 12hr  (34) white prepupae 24hr    14 pupae 2d postWPP    15 pupae 3d postWPP    25 pupae 4d postWPP    18 adult male 01day  (44) adult male 05day  (33) adult male 30day  (33) adult female 01day  (36) adult female 05day    24 adult female 30day    24 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    27 embryo 04-06hr    25 embryo 06-08hr    27 embryo 08-10hr    33 embryo 10-12hr    49 embryo 12-14hr    51 embryo 14-16hr    52 embryo 16-18hr    48 embryo 18-20hr    25 embryo 20-22hr    11 embryo 22-24hr    12 larva L1    6 larva L2    12 larva L3 12hr old    11 larva L3 puffstage 1-2    30 larva L3 puffstage 3-6    49 larva L3 puffstage 7-9    85 white prepupae new    67 white prepupae 12hr    34 white prepupae 24hr    14 pupae 2d postWPP    15 pupae 3d postWPP    25 pupae 4d postWPP    18 adult male 01day    44 adult male 05day    33 adult male 30day    33 adult female 01day    36 adult female 05day    24 adult female 30day    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    27 embryo 04-06hr    25 embryo 06-08hr    27 embryo 08-10hr    33 embryo 10-12hr    49 embryo 12-14hr    51 embryo 14-16hr    52 embryo 16-18hr    48 embryo 18-20hr    25 embryo 20-22hr    11 embryo 22-24hr    12 larva L1    6 larva L2    12 larva L3 12hr old    11 larva L3 puffstage 1-2    30 larva L3 puffstage 3-6    49 larva L3 puffstage 7-9    85 white prepupae new    67 white prepupae 12hr    34 white prepupae 24hr    14 pupae 2d postWPP    15 pupae 3d postWPP    25 pupae 4d postWPP    18 adult male 01day    44 adult male 05day    33 adult male 30day    33 adult female 01day    36 adult female 05day    24 adult female 30day    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    27 Schneider line 2 Sg4    26 embryonic 1182-4H    29 embryonic GM2    205 embryonic Kc167    21 embryonic S1    14 embryonic S3    77 leg disc CME L1    20 wing disc CME-W2    20 wing disc ML-DmD8    16 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    19 wing disc ML-DmD21    8 wing disc ML-DmD32    34 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    19 antennal disc ML-DmD20-c5    30 mixed discs ML-DmD4-c1    54 CNS ML-DmBG1-c1    8 CNS ML-DmBG2-c2    70 tumorous blood cells mbn2    190 ovary fGS/OSS    4 ovary OSC    3 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (27) Schneider line 2 Sg4  26 embryonic 1182-4H  (29) embryonic GM2  (205) embryonic Kc167    21 embryonic S1    14 embryonic S3  (77) leg disc CME L1    20 wing disc CME-W2    20 wing disc ML-DmD8    16 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    19 wing disc ML-DmD21    8 wing disc ML-DmD32  (34) haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    19 antennal disc ML-DmD20-c5  (30) mixed discs ML-DmD4-c1  (54) CNS ML-DmBG1-c1    8 CNS ML-DmBG2-c2  (70) tumorous blood cells mbn2  (190) ovary fGS/OSS    4 ovary OSC    3 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    27 Schneider line 2 Sg4    26 embryonic 1182-4H    29 embryonic GM2  (205) embryonic Kc167    21 embryonic S1    14 embryonic S3    77 leg disc CME L1    20 wing disc CME-W2    20 wing disc ML-DmD8    16 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    19 wing disc ML-DmD21    8 wing disc ML-DmD32    34 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    19 antennal disc ML-DmD20-c5    30 mixed discs ML-DmD4-c1    54 CNS ML-DmBG1-c1    8 CNS ML-DmBG2-c2    70 tumorous blood cells mbn2  (190) ovary fGS/OSS    4 ovary OSC    3 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    27 Schneider line 2 Sg4    26 embryonic 1182-4H    29 embryonic GM2    205 embryonic Kc167    21 embryonic S1    14 embryonic S3    77 leg disc CME L1    20 wing disc CME-W2    20 wing disc ML-DmD8    16 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    19 wing disc ML-DmD21    8 wing disc ML-DmD32    34 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    19 antennal disc ML-DmD20-c5    30 mixed discs ML-DmD4-c1    54 CNS ML-DmBG1-c1    8 CNS ML-DmBG2-c2    70 tumorous blood cells mbn2    190 ovary fGS/OSS    4 ovary OSC    3 ovary OSS    3 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    27 Schneider line 2 Sg4    26 embryonic 1182-4H    29 embryonic GM2    205 embryonic Kc167    21 embryonic S1    14 embryonic S3    77 leg disc CME L1    20 wing disc CME-W2    20 wing disc ML-DmD8    16 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    19 wing disc ML-DmD21    8 wing disc ML-DmD32    34 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    19 antennal disc ML-DmD20-c5    30 mixed discs ML-DmD4-c1    54 CNS ML-DmBG1-c1    8 CNS ML-DmBG2-c2    70 tumorous blood cells mbn2    190 ovary fGS/OSS    4 ovary OSC    3 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  27 Schneider line 2 Sg4  26 embryonic 1182-4H  29 embryonic GM2  (205) embryonic Kc167    21 embryonic S1    14 embryonic S3  (77) leg disc CME L1    20 wing disc CME-W2    20 wing disc ML-DmD8    16 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    19 wing disc ML-DmD21    8 wing disc ML-DmD32  (34) haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    19 antennal disc ML-DmD20-c5  30 mixed discs ML-DmD4-c1  (54) CNS ML-DmBG1-c1    8 CNS ML-DmBG2-c2  (70) tumorous blood cells mbn2  (190) ovary fGS/OSS    4 ovary OSC    3 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    27 Schneider line 2 Sg4    26 embryonic 1182-4H    29 embryonic GM2  (205) embryonic Kc167    21 embryonic S1    14 embryonic S3    77 leg disc CME L1    20 wing disc CME-W2    20 wing disc ML-DmD8    16 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    19 wing disc ML-DmD21    8 wing disc ML-DmD32    34 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    19 antennal disc ML-DmD20-c5    30 mixed discs ML-DmD4-c1    54 CNS ML-DmBG1-c1    8 CNS ML-DmBG2-c2    70 tumorous blood cells mbn2  (190) ovary fGS/OSS    4 ovary OSC    3 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    27 Schneider line 2 Sg4    26 embryonic 1182-4H    29 embryonic GM2    205 embryonic Kc167    21 embryonic S1    14 embryonic S3    77 leg disc CME L1    20 wing disc CME-W2    20 wing disc ML-DmD8    16 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    19 wing disc ML-DmD21    8 wing disc ML-DmD32    34 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    19 antennal disc ML-DmD20-c5    30 mixed discs ML-DmD4-c1    54 CNS ML-DmBG1-c1    8 CNS ML-DmBG2-c2    70 tumorous blood cells mbn2    190 ovary fGS/OSS    4 ovary OSC    3 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    12 cold shock, 4-day adult    10 heat shock, 4-day adult    9 Cadmium 50 mM 6 hrs, larvae L3    12 Cadmium 50 mM 12 hrs, larvae L3    36 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    11 Ethanol 5% 3 hrs, larvae L3    20 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    20 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    8 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    8 Paraquat 10 mM 48 hrs, 4-day adult    13 Rotenone 2 μg 12 hrs, larvae L3    17 Rotenone 8 μg 12 hrs, larvae L3    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    12 cold shock, 4-day adult    10 heat shock, 4-day adult    9 Cadmium 50 mM 6 hrs, larvae L3    12 Cadmium 50 mM 12 hrs, larvae L3  (36) Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    11 Ethanol 5% 3 hrs, larvae L3    20 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    20 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    8 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    8 Paraquat 10 mM 48 hrs, 4-day adult    13 Rotenone 2 μg 12 hrs, larvae L3    17 Rotenone 8 μg 12 hrs, larvae L3    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    12 cold shock, 4-day adult    10 heat shock, 4-day adult    9 Cadmium 50 mM 6 hrs, larvae L3    12 Cadmium 50 mM 12 hrs, larvae L3    36 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    11 Ethanol 5% 3 hrs, larvae L3    20 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    20 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    8 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    8 Paraquat 10 mM 48 hrs, 4-day adult    13 Rotenone 2 μg 12 hrs, larvae L3    17 Rotenone 8 μg 12 hrs, larvae L3    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    12 cold shock, 4-day adult    10 heat shock, 4-day adult    9 Cadmium 50 mM 6 hrs, larvae L3    12 Cadmium 50 mM 12 hrs, larvae L3    36 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    11 Ethanol 5% 3 hrs, larvae L3    20 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    20 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    8 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    8 Paraquat 10 mM 48 hrs, 4-day adult    13 Rotenone 2 μg 12 hrs, larvae L3    17 Rotenone 8 μg 12 hrs, larvae L3    9 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    12 cold shock, 4-day adult    10 heat shock, 4-day adult    9 Cadmium 50 mM 6 hrs, larvae L3    12 Cadmium 50 mM 12 hrs, larvae L3    36 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    11 Ethanol 5% 3 hrs, larvae L3    20 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    20 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    8 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    8 Paraquat 10 mM 48 hrs, 4-day adult    13 Rotenone 2 μg 12 hrs, larvae L3    17 Rotenone 8 μg 12 hrs, larvae L3    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    12 cold shock, 4-day adult    10 heat shock, 4-day adult    9 Cadmium 50 mM 6 hrs, larvae L3    12 Cadmium 50 mM 12 hrs, larvae L3  (36) Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    11 Ethanol 5% 3 hrs, larvae L3    20 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    20 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    8 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    8 Paraquat 10 mM 48 hrs, 4-day adult    13 Rotenone 2 μg 12 hrs, larvae L3    17 Rotenone 8 μg 12 hrs, larvae L3    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    12 cold shock, 4-day adult    10 heat shock, 4-day adult    9 Cadmium 50 mM 6 hrs, larvae L3    12 Cadmium 50 mM 12 hrs, larvae L3    36 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    11 Ethanol 5% 3 hrs, larvae L3    20 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    20 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    8 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    8 Paraquat 10 mM 48 hrs, 4-day adult    13 Rotenone 2 μg 12 hrs, larvae L3    17 Rotenone 8 μg 12 hrs, larvae L3    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    12 cold shock, 4-day adult    10 heat shock, 4-day adult    9 Cadmium 50 mM 6 hrs, larvae L3    12 Cadmium 50 mM 12 hrs, larvae L3    36 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    11 Ethanol 5% 3 hrs, larvae L3    20 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    20 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    8 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    8 Paraquat 10 mM 48 hrs, 4-day adult    13 Rotenone 2 μg 12 hrs, larvae L3    17 Rotenone 8 μg 12 hrs, larvae L3    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_27 mE1_20_mRNA_expression_cluster_03
External Data & Images
Linkouts	BDGP expression data • GH23453 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0050344 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG30344-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for CG30344 Stages(s) 4-6 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable, with Scer\GAL4pnr-MD237 CG30344GD1890 Phenotype manifest in Allele
Classical Alleles ( 0 )
For All Classical Alleles Show
Allele of CG30344 Class Mutagen Stocks Known lesion
Alleles Carried on Transgenic Constructs ( 2 )
For All Alleles Carried on Transgenic Constructs Show
Allele of CG30344 Class Mutagen Stocks Known lesion CG30344GD1890 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes CG30344KK110555 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD1890} NA P{KK110555} NA
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 4 unique terms )
Terms Based on Experimental Evidence ( 1 term )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
response to methotrexate inferred from expression pattern (Chahine and O'Donnell, 2010, Chahine and O'Donnell, 2009)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 3 terms )
Molecular Function
CV term Evidence References
transporter activity inferred from electronic annotation with InterPro:IPR005829 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
transmembrane transport inferred from electronic annotation with InterPro:IPR005829, InterPro:IPR011701 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
integral to membrane inferred from electronic annotation with InterPro:IPR011701 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
CG30344 - CG10566 experimental knowledge based (Guruharsha et al., 2011) CG30344 - CG3344 experimental knowledge based (Guruharsha et al., 2011) CG30344 - CG7239 experimental knowledge based (Guruharsha et al., 2011) CG30344 - Ca-P60A experimental knowledge based (Guruharsha et al., 2011) CG30344 - Cyp4ae1 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) CG30344 - Ice anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG30344 - KdelR anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG30344 - Rab6 experimental knowledge based (Guruharsha et al., 2011) CG30344 - Surf4 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG30344 - Teh1 experimental knowledge based (Guruharsha et al., 2011) CG30344 - Timp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG30344 - eIF-4B experimental knowledge based (Guruharsha et al., 2011) CG30344 - igl anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG30344 - pps experimental knowledge based (Guruharsha et al., 2011) CG30344 - tmod experimental knowledge based (Guruharsha et al., 2011)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions CG30344 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 73116 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0050344 DroID - A comprehensive database of gene and protein interactions. • FBgn0050344 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 246552
Orthologs
OrthoDB Orthologs (42) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6KD75S Dipteran inclusive ortholog search EOG6NGGW2 Insect inclusive ortholog search EOG6PRRMJ Arthropod inclusive ortholog search EOG6V41QG Metazoa inclusive ortholog search EOG6F554R, EOG60CW9C
Orthologs in Drosophila Species (EOG6KD75S)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\CG30344     Drosophila simulans   Dsim\GD10638     Drosophila sechellia   Dsec\GM21103 Y   Drosophila erecta   Dere\GG23422     Drosophila yakuba   Dyak\GE19261     Drosophila ananassae   Dana\GF11962     Drosophila pseudoobscura pseudoobscura   Dpse\GA15775     Drosophila persimilis   Dper\GL17010     Drosophila willistoni   Dwil\GK23342     Drosophila willistoni   Dwil\GK23339     Drosophila virilis   Dvir\GJ17523     Drosophila mojavensis   Dmoj\GI19716     Drosophila grimshawi   Dgri\GH22011
Orthologs in non-Drosophila Dipterans (EOG6NGGW2)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL001699 Y Aedes aegypti Yellow fever mosquito  Aaeg\AAEL001691 Y Anopheles gambiae Malaria mosquito  Agam\AGAP005317 Y Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ015155 Y Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ015153 Y
Orthologs in non-Dipteran Insects (EOG6PRRMJ)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB49339 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG000129 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG000127 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH12465 Y Atta cephalotes Leafcutter ant  Acep\ACEP14460 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO16643 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL15355 Y Linepithema humile Argentine ant  Lhum\LH20309 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB16999 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB23265 Y Solenopsis invicta Red fire ant  Sinv\SINV16683 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI002425 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI080681 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI072777 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI23467 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI50787 Y Bombyx mori Silkmoth  Bmor\BGIBMGA014434 Y Bombyx mori Silkmoth  Bmor\BGIBMGA004081 Y Pediculus humanus Human body louse  Phum\PHUM424170 Y
Orthologs in non-Insect Arthropods (None identified)
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (EOG6F554R, EOG60CW9C)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_025146gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_018750gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_012775gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_008807gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_008808gn Y
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (1) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila sechellia Dsec\GM21103
Stocks & Reagents
Stocks Listed in FlyBase ( 3 )
VDRC	v106870 P{KK110555}VIE-260B v7378 w1118; P{GD1890}v7378 v7379 w1118; P{GD1890}v7379
Genomic Clones ( 1 )
	BACR10M14 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 31 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH23453
Other clones	DPiM_GH23453
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH15988 RE03369 RE04450 RE18122 RE22469 RE39392 RE57909 RE57975 RH43225 RH47102 RH63669 SD15377
Other clones	47548 52798 156852 214309.43 CK01584 Dmel_dig_004227_0657_1516 EK168516 EK175560 EK259911 EP02802 EP03426 ESG011a.E7.4_F06 ESG013a.E21_G08 ESG016a.E21_D07 ESG016b.E21_D05 IP12171 IP18531
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0050344 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 246552
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments	Annotation CG8054 split into CG30342 and CG30344 in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003)
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_165655 RefSeq (Proteins) • NP_724759 DDBJ / EMBL / GenBank • AAK93014 AAM68813 AI403908 AW940906 AY051590 Entrez Gene - A searchable database of RefSeq genes. • 246552 UniProtKB/TrEMBL • Q961H2
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Sugar transporter, conserved site (IPR005829) Major facilitator superfamily (IPR011701) Major facilitator superfamily domain, general substrate transporter (IPR016196) Major facilitator superfamily domain (IPR020846) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • GH23453
Linkouts
	BioGRID - A database of protein and genetic interactions • 73116 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0050344 DroID - A comprehensive database of gene and protein interactions. • FBgn0050344 DRSC - Results from RNAi screens. • FBgn0050344 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0050344 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG30344-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0050344 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 246552 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 246552 modMine - Data generated by the modENCODE project. • FBgn0050344
Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	CG8054 (Freeman et al., 2003, Wang et al., 2004, Roxstrom-Lindquist et al., 2004) CG30344 (Wang et al., 2004, Keleman et al., 2009.8.5, Chahine and O'Donnell, 2010, Chahine and O'Donnell, 2009, Cammarato et al., 2011) MET (Chahine and O'Donnell, 2010, Chahine and O'Donnell, 2009)
Name Synonym	multidrug efflux transporter (Chahine and O'Donnell, 2010, Chahine and O'Donnell, 2009)
Secondary FlyBase IDs
	FBgn0033386
References ( 23 )
Generate a list of	All references relating to this gene ( 23 )
List References by type	Research paper  ( 7 ) Supplementary material  ( 6 ) Personal communication to FlyBase  ( 5 ) FlyBase analysis  ( 2 ) Website  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 1 )
	Chahine et al., 2012, Arch. Insect Biochem. Physiol. 81(4): 228--240 EFFECTS OF GENETIC KNOCK-DOWN OF ORGANIC ANION TRANSPORTER GENES ON SECRETION OF FLUORESCENT ORGANIC IONS BY MALPIGHIAN TUBULES OF Drosophila melanogaster. [FBrf0220031] Show all research papers ...