FlyBase Gene Report: Dmel\CG30394

General Information

Symbol

Dmel\CG30394

Species

D. melanogaster

Name

Annotation Symbol

CG30394

Feature Type

protein_coding_gene

FlyBase ID

FBgn0050394

Gene Model Status

Current

Stock Availability

4 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

57D11-57D11

Sequence location

2R:21,320,040..21,323,320 [-]

Recombination map

2-94

RefSeq locus

NT_033778 REGION:21320040..21323320

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (2 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables amino acid transmembrane transporter activity

inferred from sequence or structural similarity with FLYBASE:Eaat1; FB:FBgn0026439

(Romero-Calderon et al., 2007)

inferred from biological aspect of ancestor with PANTHER:PTN000535793

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in amino acid transmembrane transport

inferred from biological aspect of ancestor with PANTHER:PTN000535793

(assigned by GO_Central )

(Gaudet et al., 2011)

inferred from sequence or structural similarity with FLYBASE:Eaat1; FB:FBgn0026439

(Romero-Calderon et al., 2007)

Cellular Component (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

SLC38 FAMILY OF SODIUM-COUPLED NEUTRAL AMINO ACID TRANSPORTERS

Protein Family (UniProt)

Protein Signatures (InterPro)

Amino acid transporter, transmembrane domain

Summaries

Gene Group (FlyBase)

SLC38 FAMILY OF SODIUM-COUPLED NEUTRAL AMINO ACID TRANSPORTERS -: Solute carrier family 38 (SLC38) or sodium-coupled neutral amino acid transporters (SNAT) mediate the transmembrane transport of glutamine, asparagine, histidine and small, aliphatic amino acids. (Adapted from PMID:12845534 and PMID:23506890).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\CG30394 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.42

Gene model reviewed during 5.51

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

CG30394-RA

FBtr0071614

NM_137735

3055

831

CG30394-RB

FBtr0071613

NM_137734

2959

831

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

CG30394-PA

FBpp0071540

91.3

831

5.51

NP_611579

AAF46715

CG30394-PB

FBpp0071539

91.3

831

5.51

NP_611578

AAF46714

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

831 aa isoforms: CG30394-PA, CG30394-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Amino acid transporter, transmembrane domain

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CG30394 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG30394_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism | ubiquitous

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

garland cell primordium

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

salivary gland body primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic proventriculus

(Fisher et al., 2012)

embryonic proventriculus inner layer

(Fisher et al., 2012)

embryonic/larval garland cell

(Fisher et al., 2012)

embryonic/larval salivary gland

(Fisher et al., 2012)

embryonic/larval salivary gland body

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

(Romero-Calderon et al., 2007)

adult thorax | faint

Comment: Thorax and abdomen assayed together.

(Romero-Calderon et al., 2007)

adult abdomen | faint

Comment: Thorax and abdomen assayed together.

(Romero-Calderon et al., 2007)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\CG30394 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\CG30394

View exonic expression by tissue for Dmel\CG30394

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0050394

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0050394

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG30394-RB

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG30394

Flygut - An atlas of the Drosophila adult midgut

FBgn0050394

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\CG30394

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 5 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of CG30394

Transgenic constructs containing regulatory region of CG30394

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene disrupted in

Df(2R)Exel6074

(Ryder, 2004.4.26)

Df(2R)Exel6075

(Ryder, 2004.4.26)

Inferred from location ( 0 )

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

CG30394^GD2127

viable, with Scer\GAL4^Mef2.PR

CG30394^GD2127

viable, with Scer\GAL4^pnr-MD237

CG30394^GD2127

viable, with Scer\GAL4^tin.CΔ4

CG30394^GD2127

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (16)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\SLC38A10

11 of 15

Yes

Hsap\SLC38A1

3 of 15

Yes

Hsap\SLC38A2

3 of 15

Yes

Hsap\SLC38A6

3 of 15

Yes

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (16)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Slc38a10

10 of 15

Yes

Mmus\Slc38a1

3 of 15

Yes

Mmus\Slc38a2

3 of 15

Yes

Mmus\Slc38a6

3 of 15

Yes

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Rattus norvegicus (Norway rat) (16)

Rnor\Slc38a10

7 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Xenopus tropicalis (Western clawed frog) (12)

Xtro\slc38a10

6 of 12

Yes

Xtro\slc38a6

3 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Danio rerio (Zebrafish) (18)

Drer\slc38a10

9 of 15

Yes

Drer\slc38a2

3 of 15

Yes

Drer\slc38a5a

3 of 15

Yes

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

Drer\si:dkey-126h10.1

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (11)

Cele\Y51F10.4

9 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Arabidopsis thaliana (thale-cress) (5)

Atha\AT1G80510

2 of 9

Yes

Atha\AT2G40420

2 of 9

Yes

Atha\AT3G30390

2 of 9

Yes

Atha\AT3G56200

2 of 9

Yes

Atha\AT5G38820

1 of 9

Saccharomyces cerevisiae (Brewer's yeast) (7)

Scer\AVT6

4 of 15

Yes

Scer\AVT5

3 of 15

Yes

Scer\AVT7

3 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\avt5

4 of 12

Yes

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190356 )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA15814

Drosophila persimilis

Dper\GL10750

Drosophila willistoni

Dwil\GK21706

Drosophila virilis

Dvir\GJ22382

Drosophila mojavensis

Dmoj\GI20533

Drosophila grimshawi

Dgri\GH21245

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091505NE )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17021665

Glossina morsitans

Tsetse fly

Gmor\GMOY004385

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_00310

Mayetiola destructor

Hessian fly

Mdes\Mdes010895

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL001114

Anopheles darlingi

American malaria mosquito

Adar\ADAC010009

Anopheles gambiae

Malaria mosquito

Agam\AGAP001923

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ007329

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W05LG )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA012053

Danaus plexippus

Monarch butterfly

Dple\DPOGS214820-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL004971

Apis florea

Little honeybee

Aflo\2597403828

Apis mellifera

Western honey bee

Amel\LOC552798

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP25290

Bombus terrestris

Buff-tailed bumblebee

Bter\15207352

Linepithema humile

Argentine ant

Lhum\LH17251

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:0027ef

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG020715

Tribolium castaneum

Red flour beetle

Tcas\TC003653

Pediculus humanus

Human body louse

Phum\PHUM158020

Rhodnius prolixus

Kissing bug

Rpro\RPRC010239

Cimex lectularius

Bed bug

Clec\CLEC004573

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI000627

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI089276

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI082216

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_05942

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X05HL )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR007933

Stegodyphus mimosarum

African social velvet spider

Smim\KFM74121.1

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_304718

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0IDE )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-AAT-aminoacidtransporter

Gallus gallus

Domestic chicken

Ggal\SLC38A10

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (14)

Gene Symbol

Score

Source

Alignment

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

mah

3 of 10

3 of 10

3 of 10

3 of 10

2 of 10

2 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

SLC38A10; solute carrier family 38 member 10

11 of 15

616525

SLC38A1; solute carrier family 38 member 1

3 of 15

608490

SLC38A2; solute carrier family 38 member 2

3 of 15

605180

SLC38A6; solute carrier family 38 member 6

3 of 15

616518

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0050394

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

37437

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-94

Cytogenetic map

57D11-57D11

Sequence location

2R:21,320,040..21,323,320 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

57D11-57D11

Limits computationally determined from genome sequence between P{EP}CG4266^EP2258 and P{EP}CG30394^EP962&P{lacW}dom^k08108

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-94

(FlyBase, 2016)

2-101.3

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (4)

Bloomington

55589

y¹ w^*; Mi{MIC}CG30394^MI11189

Kyoto

104727

w^*; P{GawB}CG30394^NP4644 / CyO

VDRC

v330312

P{VSH330312}attP40

v3470

w¹¹¹⁸; P{GD2127}v3470

Genomic Clones (12)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (18)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0050394

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0050394

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

37437

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: CG30394 anon-WO0172774.187 anon-WO0172774.188

(FlyBase, 1996-)

Source for merge of: CG10500 CG15670

(FlyBase Genome Annotators, 2002-2003)

Additional comments

Annotations CG10500 and CG15670 merged as CG30394 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Source for merge of CG30394 anon-WO0172774.187 anon-WO0172774.188 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Other Comments

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 38 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

37437

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0050394

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0050394

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG30394_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG30394

Flygut - An atlas of the Drosophila adult midgut

FBgn0050394

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

37437

InterPro - A database of protein families, domains and functional sites

Amino acid transporter, transmembrane domain

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG30394

modMine - A data warehouse for the modENCODE project

FBgn0050394

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0050394

DRSC - Results frm RNAi screens

FBgn0050394

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0050394

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG30394-RB

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0050394

FlyMine - An integrated database for Drosophila genomics

FBgn0050394

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

37437

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

CG10500

CG15670

CG30394

(Aggarwal et al., 2019, Yang et al., 2018, Meadows, 2017.1.11, Limmer et al., 2014, Japanese National Institute of Genetics, 2012.5.21, Featherstone, 2011, Grieder et al., 2007, Romero-Calderon et al., 2007)

anon-WO0172774.187

anon-WO0172774.188

Name Synonyms

Secondary FlyBase IDs

FBgn0034619
FBgn0034620
FBgn0046415
FBgn0046416

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (33)

The following external sites may use different assemblies or annotations than FlyBase.

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