FB2020_06, released Dec 22, 2020

Gene: Dmel\ave

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General Information
Symbol
Dmel\ave
Species
D. melanogaster
Name
aveugle
Annotation Symbol
CG30476
Feature Type
protein_coding_gene
FlyBase ID
FBgn0050476
Gene Model Status
Current
Stock Availability
4 publicly available
Gene Snapshot
aveugle (ave) encodes an adaptor protein that binds to the scaffolding protein encoded by cnk and promotes the activation of the product of Raf by recruiting the product of ksr. [Date last reviewed: 2019-03-07]
Other Summaries
Also Known As

HYP

Key Links
Genomic Location
Cytogenetic map
51C2-51C2
Sequence location
2R:14,752,521..14,753,269 [+]
Recombination map

2-72

RefSeq locus
NT_033778 REGION:14752521..14753269
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (11 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables scaffold protein binding
inferred from physical interaction with FLYBASE:ksr; FB:FBgn0015402, FLYBASE:cnk; FB:FBgn0286070
(Douziech et al., 2006)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (7 terms)
Terms Based on Experimental Evidence (7 terms)
CV Term
Evidence
References
involved_in compound eye cone cell differentiation
inferred from mutant phenotype
(Roignant et al., 2006)
involved_in compound eye photoreceptor cell differentiation
inferred from mutant phenotype
(Roignant et al., 2006)
involved_in epidermal growth factor receptor signaling pathway
inferred from mutant phenotype
(Roignant et al., 2006)
involved_in eye photoreceptor cell differentiation
inferred from mutant phenotype
(assigned by UniProt )
(Roignant et al., 2006)
involved_in positive regulation of ERK1 and ERK2 cascade
inferred from mutant phenotype
(Wolfstetter et al., 2017)
inferred from direct assay
(Douziech et al., 2006)
involved_in positive regulation of Ras protein signal transduction
inferred from high throughput genetic interaction with FLYBASE:Ras85D; FB:FBgn0003205
(Ashton-Beaucage et al., 2014)
involved_in transmembrane receptor protein tyrosine kinase signaling pathway
inferred from mutant phenotype
(Wolfstetter et al., 2017)
Terms Based on Predictions or Assertions (0 terms)
Cellular Component (3 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
located_in cytoplasmic side of plasma membrane
inferred from direct assay
(Roignant et al., 2006)
located_in cytoplasm
inferred from direct assay
(assigned by UniProt )
(Roignant et al., 2006)
located_in cytosol
inferred from direct assay
(Roignant et al., 2006)
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Pathway (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Pathway (FlyBase)
EGFR Signaling Pathway Core Components -
The Epidermal Growth Factor Receptor (EGFR) signaling pathway is used multiple times during development (FBrf0190321). It is activated by the binding of a secreted ligand to the receptor tyrosine kinase Egfr and acts via the canonical Ras/Raf/MAP kinase (ERK) cascade. (Adapted from FBrf0190321 and FBrf0221727).
Protein Function (UniProtKB)
Required for normal photoreceptor differentiation between Ras and Raf for EGFR signaling in the eye and for mitogen-activated protein kinase phosphorylation. Probably acts together with Cnk to promote Raf activation, perhaps by recruiting an activating kinase.
(UniProt, Q8ML92)
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\ave for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.50

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
ave-RA
FBtr0087439
NM_166066
546
106
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
ave-PA
FBpp0086569
12.8
106
9.77
NP_725413
AAM68548
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Interacts with the SAM domain of cnk.

(UniProt, Q8ML92)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ave using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in cytoplasmic side of plasma membrane
inferred from direct assay
(Roignant et al., 2006)
located_in cytoplasm
inferred from direct assay
(assigned by UniProt )
(Roignant et al., 2006)
located_in cytosol
inferred from direct assay
(Roignant et al., 2006)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\ave in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 4 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of ave
Transgenic constructs containing regulatory region of ave
Deletions and Duplications ( 2 )
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (4)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\SAMD10
9 of 15
Yes
Yes
1  
Hsap\SAMD12
9 of 15
Yes
Yes
Hsap\HBB
1 of 15
No
No
0  
Hsap\SAMD15
1 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Samd12
12 of 15
Yes
Yes
Mmus\Samd10
8 of 15
No
Yes
Rattus norvegicus (Norway rat) (2)
Rnor\Samd10
6 of 13
Yes
Yes
Rnor\Samd12
5 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (2)
Xtro\LOC101731956
2 of 12
Yes
Yes
Xtro\samd12
2 of 12
Yes
Yes
Danio rerio (Zebrafish) (3)
Drer\samd10b
10 of 15
Yes
Yes
Drer\samd10a
7 of 15
No
Yes
Drer\samd12
3 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (2)
Cele\ave-1
11 of 15
Yes
Yes
Cele\C05D10.4
1 of 15
No
Yes
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190J68 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00002560
Drosophila simulans
Dsim\GD11074
Drosophila sechellia
Dsec\GM21568
Drosophila erecta
Dere\GG20478
Drosophila yakuba
Dyak\GE13608
Drosophila ananassae
Dana\GF11340
Drosophila pseudoobscura pseudoobscura
Dpse\GA15868
Drosophila persimilis
Dper\GL11459
Drosophila willistoni
Dwil\GK19633
Drosophila virilis
Dvir\GJ20573
Drosophila mojavensis
Dmoj\GI20838
Drosophila grimshawi
Dgri\GH20753
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150G1L )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17019868
Glossina morsitans
Tsetse fly
Gmor\GMOY000455
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_09388
Mayetiola destructor
Hessian fly
Mdes\Mdes005134
Mayetiola destructor
Hessian fly
Mdes\Mdes010972
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL013728
Anopheles darlingi
American malaria mosquito
Adar\ADAC003937
Anopheles gambiae
Malaria mosquito
Agam\AGAP012177
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ002192
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0LC0 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA010954
Danaus plexippus
Monarch butterfly
Dple\DPOGS215271-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL011989
Apis florea
Little honeybee
Aflo\2230625300
Apis mellifera
Western honey bee
Amel\ave
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP17710
Bombus terrestris
Buff-tailed bumblebee
Bter\15213319
Linepithema humile
Argentine ant
Lhum\LH12544
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:000741
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG002728
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_12702
Tribolium castaneum
Red flour beetle
Tcas\TC005991
Rhodnius prolixus
Kissing bug
Rpro\RPRC010305
Cimex lectularius
Bed bug
Clec\CLEC002374
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI005987
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_04911
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0LJK )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR010062
Stegodyphus mimosarum
African social velvet spider
Smim\KFM62770.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur01g13240
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_92909
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0YT6 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\SPU_027075gn
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000003778
Gallus gallus
Domestic chicken
Ggal\SAMD12
Gallus gallus
Domestic chicken
Ggal\SAMD10
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (0)
No records found.
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 1 )
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    SAMD10; sterile alpha motif domain containing 10
    9 of 15
         
        SAMD12; sterile alpha motif domain containing 12
        9 of 15
        618073
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        esyN Network Diagram
        Show neighbor-neighbor interactions:
        Select Layout:
        Legend:
        Protein
        RNA
        Selected Interactor(s)
        Interactions Browser
        View in Interactions Browser
        Please see the Physical Interaction reports below for full details
        protein-protein
        Physical Interaction
        Assay
        References
        ave - cnk
        two hybrid
        (Wolfstetter et al., 2017)
        Summary of Genetic Interactions
        esyN Network Diagram
        esyN Network Key:
        Suppression
        Enhancement
        View in Interactions Browser
        Please look at the allele data for full details of the genetic interactions
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        ave
        suppressible
        Raf
        (Roignant et al., 2006)
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Ras85D
        suppressible
        ave
        (Roignant et al., 2006)
        External Data
        Subunit Structure (UniProtKB)
        Interacts with the SAM domain of cnk.
        (UniProt, Q8ML92 )
        Linkouts
        DroID - A comprehensive database of gene and protein interactions.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        EGFR Signaling Pathway Core Components -
        The Epidermal Growth Factor Receptor (EGFR) signaling pathway is used multiple times during development (FBrf0190321). It is activated by the binding of a secreted ligand to the receptor tyrosine kinase Egfr and acts via the canonical Ras/Raf/MAP kinase (ERK) cascade. (Adapted from FBrf0190321 and FBrf0221727).
        Metabolic Pathways
        External Data
        Linkouts
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        2R
        Recombination map

        2-72

        Cytogenetic map
        51C2-51C2
        Sequence location
        2R:14,752,521..14,753,269 [+]
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        51C2-51C2
        Limits computationally determined from genome sequence between P{lacW}Rpn6k00103 and P{PZ}chn02064
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        Experimentally Determined Recombination Data
        Location

        2-72

        (FlyBase, 2016)

        2-76.4

        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (4)
        Genomic Clones (23)
        cDNA Clones (9)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        Antibody Information
        Laboratory Generated Antibodies
         
        Commercially Available Antibodies
         
        Other Information
        Relationship to Other Genes
        Source for database identify of

        Source for identity of: ave CG30476

        (Roignant, 2006.3.6)
        Source for database merge of

        Source for merge of: CG30476 BcDNA:RE67675

        (FlyBase, 1996-)
        Additional comments

        Source for merge of CG30476 BcDNA:RE67675 was a shared cDNA ( date:030728 ).

        (FlyBase, 1996-)
        Other Comments

        dsRNA made from templates generated with primers directed against this gene.

        (Douziech et al., 2006)

        The ave protein recruits ksrto cnk-bound phl. ksr is a central component of the phl activation process.

        (Douziech et al., 2006)

        ave encodes a small SAM domain protein that can physically interact with the scaffold protein cnk.

        (Roignant et al., 2006)

        ave is required between Ras85D and phl for EGFR signaling in the eye and for MAP kinase phosphorylation in cell culture.

        (Roignant et al., 2006)

        ave acts downstream of Ras85D, but upstream of phl and rl in the EGFR pathway.

        (Roignant et al., 2006)

        New annotation (CG30476) in release 3 of the genome annotation.

        (FlyBase Genome Annotators, 2002-2003)
        Origin and Etymology
        Discoverer
        Etymology

        Named "Hyphen" based on the presumed ability of the product to functionally link critical proteins within the Ras/MAPK pathway.

        (Douziech et al., 2006)
        Identification

        In a mosaic genetic screen for genes required for photoreceptor differentiation.

        (Roignant et al., 2006)

        dsRNA has been made from templates generated with primers directed against this gene.

        (Roignant et al., 2006)
        External Crossreferences and Linkouts ( 28 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
        Other crossreferences
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
        Flygut - An atlas of the Drosophila adult midgut
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        InterPro - A database of protein families, domains and functional sites
        KEGG Genes - Molecular building blocks of life in the genomic space.
        modMine - A data warehouse for the modENCODE project
        Linkouts
        DPiM - Drosophila Protein interaction map
        DroID - A comprehensive database of gene and protein interactions.
        DRSC - Results frm RNAi screens
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
        FlyMine - An integrated database for Drosophila genomics
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        Synonyms and Secondary IDs (9)
        Datasets (0)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        References (38)