FB2022_02, released March 29, 2022

Gene: Dmel\mesh

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General Information
Symbol
Dmel\mesh
Species
D. melanogaster
Name
mesh
Annotation Symbol
CG31004
Feature Type
protein_coding_gene
FlyBase ID
FBgn0051004
Gene Model Status
Current
Stock Availability
5 publicly available
Gene Summary
Required, together with Ssk and Tsp2A, for the proper organization of smooth septate junctions (sSJs), probably by mediating cell adhesion via its homophilic interaction (PubMed:22854041, PubMed:26848177). Also required for the correct subcellular localization of several sSJ components, such as Ssk, cora and l(2)gl, and for the barrier function of the midgut epithelium (PubMed:22854041). Required for maintaining the three-layered structure of the proventriculus (PubMed:22854041). (UniProt, Q0KHY3)
Contribute a Gene Snapshot for this gene.
All Summaries
Key Links
Genomic Location
Cytogenetic map
100B5-100B5
Sequence location
Recombination map
3-102
RefSeq locus
NT_033777 REGION:31178389..31195762
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (6 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables protein binding
inferred from physical interaction with UniProtKB:O46101
(assigned by UniProt )
(Izumi et al., 2016)
inferred from physical interaction with UniProtKB:Q9VW87
(assigned by UniProt )
(Izumi et al., 2012)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
involved_in smooth septate junction assembly
inferred from mutant phenotype
(Izumi et al., 2012)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in cell-matrix adhesion
inferred from electronic annotation with InterPro:IPR003886
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
Cellular Component (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
located_in apicolateral plasma membrane
inferred from direct assay
(Izumi et al., 2021)
located_in smooth septate junction
inferred from direct assay
(Izumi et al., 2021, Izumi et al., 2012)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
is_active_in extracellular space
inferred from biological aspect of ancestor with PANTHER:PTN004437744
(assigned by GO_Central )
(Gaudet et al., 2011)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Protein Function (UniProtKB)
Required, together with Ssk and Tsp2A, for the proper organization of smooth septate junctions (sSJs), probably by mediating cell adhesion via its homophilic interaction (PubMed:22854041, PubMed:26848177). Also required for the correct subcellular localization of several sSJ components, such as Ssk, cora and l(2)gl, and for the barrier function of the midgut epithelium (PubMed:22854041). Required for maintaining the three-layered structure of the proventriculus (PubMed:22854041).
(UniProt, Q0KHY3)
Summary (Interactive Fly)

transmembrane protein - component of smooth septate junctions, functions cooperates with Tetraspanin 2A and Snakeskin to organize septate junctions - midgut - Malpighian tubules

Gene Model and Products
Number of Transcripts
5
Number of Unique Polypeptides
3

Please see the JBrowse view of Dmel\mesh for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure New Section
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q0KHY3)

If you don't see the viewer to the right, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.53

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
mesh-RA
FBtr0085779
NM_170539
4734
1431
mesh-RB
FBtr0085780
NM_170540
4731
1431
mesh-RC
FBtr0300960
NM_001170310
4008
1243
mesh-RD
FBtr0300961
NM_001170311
4803
1454
mesh-RE
FBtr0344066
NM_001300689
3949
1243
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
mesh-PA
FBpp0085140
162.4
1431
6.17
NP_733418
AAF57124
mesh-PB
FBpp0085141
162.4
1431
6.17
NP_733419
AAF57123
mesh-PC
FBpp0290182
141.8
1243
6.74
NP_001163781
ACZ95074
mesh-PD
FBpp0290183
165.0
1454
6.23
NP_001163782
ACZ95075
mesh-PE
FBpp0310483
141.8
1243
6.74
NP_001287618
AHN57617
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1431 aa isoforms: mesh-PA, mesh-PB
1243 aa isoforms: mesh-PC, mesh-PE
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Forms a complex with Ssk and Tsp2A (PubMed:26848177). Interacts with Ssk; the interaction may be necessary for the localization of both proteins to the cell apicolateral region (PubMed:22854041).

(UniProt, Q0KHY3)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\mesh using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 13 -- 16
embryonic hindgut
(Fisher et al., 2012)
embryonic Malpighian tubule
(Fisher et al., 2012)
embryonic proventriculus
(Fisher et al., 2012)
embryonic/larval midgut
(Fisher et al., 2012)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\mesh in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 5 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of mesh
Transgenic constructs containing regulatory region of mesh
Aberrations (Deficiencies and Duplications) ( 3 )
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (5)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\SUSD2
13 of 15
Yes
Yes
Hsap\MUC4
3 of 15
No
Yes
Hsap\RTL9
1 of 15
No
Yes
Hsap\SNED1
1 of 15
No
Yes
Hsap\TECTA
1 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (6)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Susd2
13 of 15
Yes
Yes
Mmus\Muc4
2 of 15
No
Yes
Mmus\Adgrl4
1 of 15
No
No
Mmus\Rtl9
1 of 15
No
Yes
Mmus\Sned1
1 of 15
No
Yes
Mmus\Tecta
1 of 15
No
No
Rattus norvegicus (Norway rat) (6)
Rnor\Susd2
9 of 13
Yes
Yes
Rnor\Muc4
1 of 13
No
Yes
Rnor\Nid1
1 of 13
No
No
Rnor\Rtl9
1 of 13
No
Yes
Rnor\Sned1
1 of 13
No
Yes
Rnor\Tecta
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (3)
Xtro\muc4
2 of 12
Yes
Yes
Xtro\susd2
1 of 12
No
Yes
Xtro\tecta.1
1 of 12
No
No
Danio rerio (Zebrafish) (11)
Drer\im:7156467
7 of 15
Yes
Yes
Drer\LOC101882496
1 of 15
No
Yes
Drer\si:ch73-105b23.6
1 of 15
No
Yes
Drer\si:ch73-181m17.1
1 of 15
No
Yes
Drer\si:ch73-329n5.6
1 of 15
No
Yes
Drer\si:ch211-14k19.8
1 of 15
No
Yes
Drer\si:ch211-39f2.3
1 of 15
No
No
Drer\si:dkey-32n7.7
1 of 15
No
No
Drer\sned1
1 of 15
No
Yes
Drer\tecta
1 of 15
No
No
Drer\zgc:112964
1 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (6)
Cele\K03H1.5
14 of 15
Yes
Yes
Cele\F54D1.6
4 of 15
No
Yes
Cele\R09E10.5
4 of 15
No
Yes
Cele\B0393.5
1 of 15
No
Yes
Cele\dex-1
1 of 15
No
Yes
Cele\nid-1
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Other Organism Orthologs (via OrthoDB)
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (1)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
Ndg
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    SUSD2; sushi domain containing 2
    13 of 15
    615825
        MUC4; mucin 4, cell surface associated
        3 of 15
        158372
            Functional Complementation Data
            Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
            Interactions
            Summary of Physical Interactions
            esyN Network Diagram
            Show neighbor-neighbor interactions:
            Select Layout:
            Legend:
            Protein
            RNA
            Selected Interactor(s)
            Interactions Browser
            View in Interactions Browser
            Please see the Physical Interaction reports below for full details
            protein-protein
            Physical Interaction
            Assay
            References
            mesh - Ssk
            anti bait coimmunoprecipitation, western blot
            (Izumi et al., 2021, Izumi et al., 2012)
            mesh - Tsp2A
            anti tag coimmunoprecipitation, western blot
            (Izumi et al., 2016)
            mesh - hoka
            anti bait coimmunoprecipitation, western blot
            (Izumi et al., 2021)
            mesh - yki
            anti tag coimmunoprecipitation, anti tag western blot
            (Chen et al., 2020)
            Summary of Genetic Interactions
            esyN Network Diagram
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            External Data
            Subunit Structure (UniProtKB)
            Forms a complex with Ssk and Tsp2A (PubMed:26848177). Interacts with Ssk; the interaction may be necessary for the localization of both proteins to the cell apicolateral region (PubMed:22854041).
            (UniProt, Q0KHY3 )
            Linkouts
            DroID - A comprehensive database of gene and protein interactions.
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Pathways
            Signaling Pathways (FlyBase)
            Metabolic Pathways
            FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
            External Data
            Linkouts
            Genomic Location and Detailed Mapping Data
            Chromosome (arm)
            3R
            Recombination map
            3-102
            Cytogenetic map
            100B5-100B5
            Sequence location
            FlyBase Computed Cytological Location
            Cytogenetic map
            Evidence for location
            100B5-100B5
            Limits computationally determined from genome sequence between P{PZ}Aph-407028&P{PZ}dcorK215 and P{PZ}l(3)rM731rM731
            Experimentally Determined Cytological Location
            Cytogenetic map
            Notes
            References
            Experimentally Determined Recombination Data
            Location

            3-102

            (FlyBase, 2016)

            3-99.1

            (Comeron et al., 2012)
            Left of (cM)
            Right of (cM)
            Notes
            Stocks and Reagents
            Stocks (5)
            Genomic Clones (34)
            cDNA Clones (76)
             

            Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

            cDNA clones, fully sequenced
            BDGP DGC clones
            Other clones
            Drosophila Genomics Resource Center cDNA clones

            For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

            cDNA Clones, End Sequenced (ESTs)
            BDGP DGC clones
            Other clones
            RNAi and Array Information
            Linkouts
            DRSC - Results frm RNAi screens
            GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
            Antibody Information
            Laboratory Generated Antibodies
             

            polyclonal

            (Izumi et al., 2012)
            Commercially Available Antibodies
             
            Other Information
            Relationship to Other Genes
            Source for database identify of

            Source for identity of: mesh CG31004

            (Izumi et al., 2012)
            Source for database merge of

            Source for merge of: CG15560 CG12074

            (FlyBase, 1996-)

            Source for merge of: CG31004 anon-WO0140519.233

            (FlyBase, 1996-)
            Additional comments

            Annotations CG12074, CG15560 merged as CG31004 in release 3 of the genome annotation.

            (FlyBase Genome Annotators, 2002-2003)

            Source for merge of CG15560 CG12074 was a shared cDNA ( date:010720 ).

            (FlyBase, 1996-)

            Source for merge of CG31004 anon-WO0140519.233 was sequence comparison ( date:051113 ).

            (FlyBase, 1996-)
            Other Comments

            mesh is required for smooth septate junction formation in the midgut.

            (Izumi et al., 2012)
            Origin and Etymology
            Discoverer
            Etymology

            The gene is named 'mesh' for its immunofluorescence staining pattern in the midgut.

            (Izumi et al., 2012)
            Identification
            External Crossreferences and Linkouts ( 56 )
            Sequence Crossreferences
            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
            GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
            UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
            UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
            UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
            Other crossreferences
            AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
            BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
            Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
            DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
            EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
            Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
            FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
            Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
            Flygut - An atlas of the Drosophila adult midgut
            FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
            GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
            iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
            InterPro - A database of protein families, domains and functional sites
            KEGG Genes - Molecular building blocks of life in the genomic space.
            MARRVEL_MODEL - MARRVEL (model organism gene)
            modMine - A data warehouse for the modENCODE project
            Linkouts
            DroID - A comprehensive database of gene and protein interactions.
            DRSC - Results frm RNAi screens
            FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
            FlyMine - An integrated database for Drosophila genomics
            Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Synonyms and Secondary IDs (8)
            Datasets (5)
            Study focus (0)
            Experimental Role
            Project
            Project Type
            Title
            Study result (5)
            Result
            Result Type
            Title
            scRNAseq_2020_Cattenoz_NI_seq_clustering
            Clustering analysis of hemocytes from non-infested third instar larvae
            scRNAseq_2020_Cho_NI72LG_seq_clustering
            Clustering analysis of lymph gland cells from non-infested larvae at 72 h after egg-laying
            scRNAseq_2020_Cho_NI96LG_seq_clustering
            Clustering analysis of lymph gland cells from non-infested larvae at 96 h after egg-laying
            scRNAseq_2020_Cho_NI120LG_seq_clustering
            Clustering analysis of lymph gland cells from non-infested larvae at 120 h after egg-laying
            scRNAseq_2020_Cho_WI96LG_seq_clustering
            Clustering analysis of lymph gland cells from wasp-infested larvae at 96 h after egg-laying
            References (48)