• Home
• Tools
  • Tools Overview
  • Query Tools

    
    • QuickSearch
    • QueryBuilder
    • TermLink
    • CytoSearch
    • Interactions Browser
    • ImageBrowse
    • Find A Person
    • Google™ FlyBase
  • Genomic/Map Tools

    
    • BLAST
    • GBrowse
    • CytoSearch
    • Aberrations Maps
    • Chromosome Maps
    • Coordinates Converter
  • Retrieve/Convert

    
    • Batch Download
    • Coordinates Converter
    • ID Converter
  • Data Submission

    
    • Fast-Track Your Paper
    • Submit Personal Comm
    • Add A New Person
    • Update Person Info
  • People

    
    • Find A Person
    • Add A New Person
    • Update Person Info
• Files
  • Files Overview
  • Downloads:
  • Precomputed files
  • Archived data
  • Releases (FTP)
  • Genomes (FTP)
  • AAA data (FTP)
  • Lists:
  • Transposons (Dmel)
  • Transposons (other)
  • Vectors & Constructs
  • Anatomical Terms
  • Map Conversion
• Species
  • Phylogeny
  • Sequenced Species
  • Synteny Table
  • Color Illustrations
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All Resources
  • Model Organisms
  • Stock Collections
  • Other links:
  • BDGP
  • DGRC
  • FlyExpress
  • Interactive Fly
  • modENCODE
  • Textpresso for Fly
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • FlyBase Guides
  • Video Tutorials
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Contact FlyBase
• Archives
  • Prior Release
  • Other Archives
  • Coordinate Converter

Gene Dmel\Nnf1b

General Information
Symbol	Dmel\Nnf1b	Species	D. melanogaster
Name	Nnf1b	Annotation symbol	CG31658
Feature type	protein_coding_gene	FlyBase ID	FBgn0051658
Gene Model Status	Current	Stock availability	2 publicly available
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	21E2-21E2	Sequence location	2L:810,586..811,531 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Nnf1b is referred to in FlyBase by the symbol Dmel\Nnf1b (CG31658, FBgn0051658). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: mitotic metaphase plate congression; chromosome segregation. 5 alleles are reported. The phenotypes of these alleles are annotated with: metaphase; mesothoracic tergum. It has one annotated transcript and one annotated polypeptide. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of very low expression. Peak expression observed within 00-12 hour embryonic stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. Comments on Affy2 ProbeSet: ProbeSet 1638348_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Nnf1b. Gene sequence location is 2L:810586..811531.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2011_10
FB2012_01	Sequence features BKN33222
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 21E2-21E2   Limits computationally determined from genome sequence between P{lacW}l(2)k09610k09610&P{lacW}Sk09538a and P{PZ}l(2)1068510685&P{lacW}Tango14k00619
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Nnf1b for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Nnf1b-RA FBtr0078008  NM_164388   774   204
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Nnf1b-PA   FBpp0077673   23.7   204   4.72     NP_722652   AAN10485
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014134 AAN10485   BI617291   BT015998   EV588353
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q8IPW3
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
epitope tag Stage Tissue/Position (including subcellular localization) Reference   cell   • kinetochore (Schittenhelm et al., 2007) Comment:Localization observed in mitotic cells; assayed in cultured S2R+ cells
Additional Descriptive Data
	Subcellular localization to kinetochores of mitotic cultured S2R+ cells assayed using the transgenic allele Nnf1bScer\UAS.T:Avic\GFP-EGFP. (Schittenhelm et al., 2007)
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0051658    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0051658 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of very low expression. Peak expression observed within 00-12 hour embryonic stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0051658 expression level Developmental Stage   Expression Level embryo 00-02hr    1273 embryo 02-04hr    995 embryo 04-06hr    788 embryo 06-08hr    566 embryo 08-10hr    510 embryo 10-12hr    411 embryo 12-14hr    347 embryo 14-16hr    247 embryo 16-18hr    222 embryo 18-20hr    105 embryo 20-22hr    249 embryo 22-24hr    268 larva L1    47 larva L2    57 larva L3 12hr old    55 larva L3 puffstage 1-2    72 larva L3 puffstage 3-6    125 larva L3 puffstage 7-9    151 white prepupae new    149 white prepupae 12hr    179 white prepupae 24hr    129 pupae 2d postWPP    133 pupae 3d postWPP    74 pupae 4d postWPP    45 adult male 01day    127 adult male 05day    137 adult male 30day    147 adult female 01day    241 adult female 05day    777 adult female 30day    740 Expression Level Scale  None   Extremely low   Very low   Low   Moderate  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (1273) embryo 02-04hr  (995) embryo 04-06hr  (788) embryo 06-08hr  (566) embryo 08-10hr  (510) embryo 10-12hr  (411) embryo 12-14hr  (347) embryo 14-16hr  (247) embryo 16-18hr  (222) embryo 18-20hr  105 embryo 20-22hr  (249) embryo 22-24hr  (268) larva L1    47 larva L2    57 larva L3 12hr old    55 larva L3 puffstage 1-2    72 larva L3 puffstage 3-6  (125) larva L3 puffstage 7-9  (151) white prepupae new  (149) white prepupae 12hr  (179) white prepupae 24hr  (129) pupae 2d postWPP  (133) pupae 3d postWPP    74 pupae 4d postWPP    45 adult male 01day  (127) adult male 05day  (137) adult male 30day  (147) adult female 01day  (241) adult female 05day  (777) adult female 30day  (740) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    1273 embryo 02-04hr    995 embryo 04-06hr    788 embryo 06-08hr    566 embryo 08-10hr    510 embryo 10-12hr    411 embryo 12-14hr    347 embryo 14-16hr    247 embryo 16-18hr    222 embryo 18-20hr    105 embryo 20-22hr    249 embryo 22-24hr    268 larva L1    47 larva L2    57 larva L3 12hr old    55 larva L3 puffstage 1-2    72 larva L3 puffstage 3-6    125 larva L3 puffstage 7-9    151 white prepupae new    149 white prepupae 12hr    179 white prepupae 24hr    129 pupae 2d postWPP    133 pupae 3d postWPP    74 pupae 4d postWPP    45 adult male 01day    127 adult male 05day    137 adult male 30day    147 adult female 01day    241 adult female 05day    777 adult female 30day    740 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1273 embryo 02-04hr    995 embryo 04-06hr    788 embryo 06-08hr    566 embryo 08-10hr    510 embryo 10-12hr    411 embryo 12-14hr    347 embryo 14-16hr    247 embryo 16-18hr    222 embryo 18-20hr    105 embryo 20-22hr    249 embryo 22-24hr    268 larva L1    47 larva L2    57 larva L3 12hr old    55 larva L3 puffstage 1-2    72 larva L3 puffstage 3-6    125 larva L3 puffstage 7-9    151 white prepupae new    149 white prepupae 12hr    179 white prepupae 24hr    129 pupae 2d postWPP    133 pupae 3d postWPP    74 pupae 4d postWPP    45 adult male 01day    127 adult male 05day    137 adult male 30day    147 adult female 01day    241 adult female 05day    777 adult female 30day    740 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1273 embryo 02-04hr    995 embryo 04-06hr    788 embryo 06-08hr    566 embryo 08-10hr    510 embryo 10-12hr    411 embryo 12-14hr    347 embryo 14-16hr    247 embryo 16-18hr    222 embryo 18-20hr    105 embryo 20-22hr    249 embryo 22-24hr    268 larva L1    47 larva L2    57 larva L3 12hr old    55 larva L3 puffstage 1-2    72 larva L3 puffstage 3-6    125 larva L3 puffstage 7-9    151 white prepupae new    149 white prepupae 12hr    179 white prepupae 24hr    129 pupae 2d postWPP    133 pupae 3d postWPP    74 pupae 4d postWPP    45 adult male 01day    127 adult male 05day    137 adult male 30day    147 adult female 01day    241 adult female 05day    777 adult female 30day    740 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0051658 expression level Developmental Stage   Expression Level embryo 00-02hr    1273 embryo 02-04hr    995 embryo 04-06hr    788 embryo 06-08hr    566 embryo 08-10hr    510 embryo 10-12hr    411 embryo 12-14hr    347 embryo 14-16hr    247 embryo 16-18hr    222 embryo 18-20hr    105 embryo 20-22hr    249 embryo 22-24hr    268 larva L1    47 larva L2    57 larva L3 12hr old    55 larva L3 puffstage 1-2    72 larva L3 puffstage 3-6    125 larva L3 puffstage 7-9    151 white prepupae new    149 white prepupae 12hr    179 white prepupae 24hr    129 pupae 2d postWPP    133 pupae 3d postWPP    74 pupae 4d postWPP    45 adult male 01day    127 adult male 05day    137 adult male 30day    147 adult female 01day    241 adult female 05day    777 adult female 30day    740 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (1273) embryo 02-04hr  (995) embryo 04-06hr  (788) embryo 06-08hr  (566) embryo 08-10hr  (510) embryo 10-12hr  (411) embryo 12-14hr  (347) embryo 14-16hr  (247) embryo 16-18hr  (222) embryo 18-20hr  105 embryo 20-22hr  (249) embryo 22-24hr  (268) larva L1    47 larva L2    57 larva L3 12hr old    55 larva L3 puffstage 1-2    72 larva L3 puffstage 3-6  125 larva L3 puffstage 7-9  (151) white prepupae new  (149) white prepupae 12hr  (179) white prepupae 24hr  129 pupae 2d postWPP  133 pupae 3d postWPP    74 pupae 4d postWPP    45 adult male 01day  127 adult male 05day  137 adult male 30day  (147) adult female 01day  (241) adult female 05day  (777) adult female 30day  (740) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    1273 embryo 02-04hr    995 embryo 04-06hr    788 embryo 06-08hr    566 embryo 08-10hr    510 embryo 10-12hr    411 embryo 12-14hr    347 embryo 14-16hr    247 embryo 16-18hr    222 embryo 18-20hr    105 embryo 20-22hr    249 embryo 22-24hr    268 larva L1    47 larva L2    57 larva L3 12hr old    55 larva L3 puffstage 1-2    72 larva L3 puffstage 3-6    125 larva L3 puffstage 7-9    151 white prepupae new    149 white prepupae 12hr    179 white prepupae 24hr    129 pupae 2d postWPP    133 pupae 3d postWPP    74 pupae 4d postWPP    45 adult male 01day    127 adult male 05day    137 adult male 30day    147 adult female 01day    241 adult female 05day    777 adult female 30day    740 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1273 embryo 02-04hr    995 embryo 04-06hr    788 embryo 06-08hr    566 embryo 08-10hr    510 embryo 10-12hr    411 embryo 12-14hr    347 embryo 14-16hr    247 embryo 16-18hr    222 embryo 18-20hr    105 embryo 20-22hr    249 embryo 22-24hr    268 larva L1    47 larva L2    57 larva L3 12hr old    55 larva L3 puffstage 1-2    72 larva L3 puffstage 3-6    125 larva L3 puffstage 7-9    151 white prepupae new    149 white prepupae 12hr    179 white prepupae 24hr    129 pupae 2d postWPP    133 pupae 3d postWPP    74 pupae 4d postWPP    45 adult male 01day    127 adult male 05day    137 adult male 30day    147 adult female 01day    241 adult female 05day    777 adult female 30day    740 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1273 embryo 02-04hr    995 embryo 04-06hr    788 embryo 06-08hr    566 embryo 08-10hr    510 embryo 10-12hr    411 embryo 12-14hr    347 embryo 14-16hr    247 embryo 16-18hr    222 embryo 18-20hr    105 embryo 20-22hr    249 embryo 22-24hr    268 larva L1    47 larva L2    57 larva L3 12hr old    55 larva L3 puffstage 1-2    72 larva L3 puffstage 3-6    125 larva L3 puffstage 7-9    151 white prepupae new    149 white prepupae 12hr    179 white prepupae 24hr    129 pupae 2d postWPP    133 pupae 3d postWPP    74 pupae 4d postWPP    45 adult male 01day    127 adult male 05day    137 adult male 30day    147 adult female 01day    241 adult female 05day    777 adult female 30day    740 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0051658    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0051658 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0051658 expression level Tissue   Expression Level Larval Central Nervous System    39.1 Larval Midgut    4.8 Larval Hindgut    5.2 Larval Malpighian Tubules    6.9 Larval Fat Body    5.7 Larval Salivary Gland    5.5 Larval Trachea    21.1 Larval Carcass no informative data Adult Head    3.3 Adult Eye    1.875 Adult Brain    3.6 Adult Thoracic-Abdominal Ganglion    2.4 Adult Crop    5 Adult Midgut    16.1 Adult Hindgut    6.7 Adult Malpighian Tubules    3.5 Adult Fat Body    4.3 Adult Salivary Gland    2.8 Adult Heart    1.875 Adult VirginFemale Spermatheca    1.1 Adult InseminatedFemale Spermatheca    3 Adult Ovary    98.2 Adult Testis    20.1 Adult Male Accessory Gland    3.2 Adult Carcass    9.1 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    39.1 Larval Midgut    4.8 Larval Hindgut    5.2 Larval Malpighian Tubules    6.9 Larval Fat Body    5.7 Larval Salivary Gland    5.5 Larval Trachea    21.1 Larval Carcass no informative data Adult Head    3.3 Adult Eye    1.875 Adult Brain    3.6 Adult Thoracic-Abdominal Ganglion    2.4 Adult Crop    5 Adult Midgut    16.1 Adult Hindgut    6.7 Adult Malpighian Tubules    3.5 Adult Fat Body    4.3 Adult Salivary Gland    2.8 Adult Heart    1.875 Adult VirginFemale Spermatheca    1.1 Adult InseminatedFemale Spermatheca    3 Adult Ovary    98.2 Adult Testis    20.1 Adult Male Accessory Gland    3.2 Adult Carcass    9.1 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    39.1 Larval Midgut    4.8 Larval Hindgut    5.2 Larval Malpighian Tubules    6.9 Larval Fat Body    5.7 Larval Salivary Gland    5.5 Larval Trachea    21.1 Larval Carcass no informative data Adult Head    3.3 Adult Eye    1.875 Adult Brain    3.6 Adult Thoracic-Abdominal Ganglion    2.4 Adult Crop    5 Adult Midgut    16.1 Adult Hindgut    6.7 Adult Malpighian Tubules    3.5 Adult Fat Body    4.3 Adult Salivary Gland    2.8 Adult Heart    1.875 Adult VirginFemale Spermatheca    1.1 Adult InseminatedFemale Spermatheca    3 Adult Ovary    98.2 Adult Testis    20.1 Adult Male Accessory Gland    3.2 Adult Carcass    9.1 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    39.1 Larval Midgut    4.8 Larval Hindgut    5.2 Larval Malpighian Tubules    6.9 Larval Fat Body    5.7 Larval Salivary Gland    5.5 Larval Trachea    21.1 Larval Carcass no informative data Adult Head    3.3 Adult Eye    1.875 Adult Brain    3.6 Adult Thoracic-Abdominal Ganglion    2.4 Adult Crop    5 Adult Midgut    16.1 Adult Hindgut    6.7 Adult Malpighian Tubules    3.5 Adult Fat Body    4.3 Adult Salivary Gland    2.8 Adult Heart    1.875 Adult VirginFemale Spermatheca    1.1 Adult InseminatedFemale Spermatheca    3 Adult Ovary    98.2 Adult Testis    20.1 Adult Male Accessory Gland    3.2 Adult Carcass    9.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0051658 expression level Tissue   Expression Level Larval Central Nervous System    39.1 Larval Midgut    4.8 Larval Hindgut    5.2 Larval Malpighian Tubules    6.9 Larval Fat Body    5.7 Larval Salivary Gland    5.5 Larval Trachea    21.1 Larval Carcass no informative data Adult Head    3.3 Adult Eye    1.875 Adult Brain    3.6 Adult Thoracic-Abdominal Ganglion    2.4 Adult Crop    5 Adult Midgut    16.1 Adult Hindgut    6.7 Adult Malpighian Tubules    3.5 Adult Fat Body    4.3 Adult Salivary Gland    2.8 Adult Heart    1.875 Adult VirginFemale Spermatheca    1.1 Adult InseminatedFemale Spermatheca    3 Adult Ovary    98.2 Adult Testis    20.1 Adult Male Accessory Gland    3.2 Adult Carcass    9.1 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    39.1 Larval Midgut    4.8 Larval Hindgut    5.2 Larval Malpighian Tubules    6.9 Larval Fat Body    5.7 Larval Salivary Gland    5.5 Larval Trachea    21.1 Larval Carcass no informative data Adult Head    3.3 Adult Eye    1.875 Adult Brain    3.6 Adult Thoracic-Abdominal Ganglion    2.4 Adult Crop    5 Adult Midgut    16.1 Adult Hindgut    6.7 Adult Malpighian Tubules    3.5 Adult Fat Body    4.3 Adult Salivary Gland    2.8 Adult Heart    1.875 Adult VirginFemale Spermatheca    1.1 Adult InseminatedFemale Spermatheca    3 Adult Ovary    98.2 Adult Testis    20.1 Adult Male Accessory Gland    3.2 Adult Carcass    9.1 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    39.1 Larval Midgut    4.8 Larval Hindgut    5.2 Larval Malpighian Tubules    6.9 Larval Fat Body    5.7 Larval Salivary Gland    5.5 Larval Trachea    21.1 Larval Carcass no informative data Adult Head    3.3 Adult Eye    1.875 Adult Brain    3.6 Adult Thoracic-Abdominal Ganglion    2.4 Adult Crop    5 Adult Midgut    16.1 Adult Hindgut    6.7 Adult Malpighian Tubules    3.5 Adult Fat Body    4.3 Adult Salivary Gland    2.8 Adult Heart    1.875 Adult VirginFemale Spermatheca    1.1 Adult InseminatedFemale Spermatheca    3 Adult Ovary    98.2 Adult Testis    20.1 Adult Male Accessory Gland    3.2 Adult Carcass    9.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    39.1 Larval Midgut    4.8 Larval Hindgut    5.2 Larval Malpighian Tubules    6.9 Larval Fat Body    5.7 Larval Salivary Gland    5.5 Larval Trachea    21.1 Larval Carcass no informative data Adult Head    3.3 Adult Eye    1.875 Adult Brain    3.6 Adult Thoracic-Abdominal Ganglion    2.4 Adult Crop    5 Adult Midgut    16.1 Adult Hindgut    6.7 Adult Malpighian Tubules    3.5 Adult Fat Body    4.3 Adult Salivary Gland    2.8 Adult Heart    1.875 Adult VirginFemale Spermatheca    1.1 Adult InseminatedFemale Spermatheca    3 Adult Ovary    98.2 Adult Testis    20.1 Adult Male Accessory Gland    3.2 Adult Carcass    9.1 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass no informative data Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    3.3   NA  Eye    1.875   NA  Brain    3.6   39.1  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    2.4   NA  Crop    5   4.8  Midgut    16.1   5.2  Hindgut    6.7   6.9  Malpighian Tubules    3.5   5.7  Fat Body    4.3   5.5  Salivary Gland    2.8   NA  Heart    1.875   21.1  Trachea    NA   NA  VirginFemale Spermatheca    1.1   NA  InseminatedFemale Spermatheca    3   NA  Ovary    98.2   NA  Testis    20.1   NA  Male Accessory Gland    3.2   no informative data  Carcass    9.1 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_01
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0051658 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG31658-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele semi-lethal | pupal stage, with Scer\GAL4pnr-MD237 Nnf1bGD13477 Other Phenotypes Allele mitotic cell cycle defective Nnf1bdsRNA.cPa visible, with Scer\GAL4pnr-MD237 Nnf1bGD13477 Phenotype manifest in Allele mesothoracic tergum, with Scer\GAL4pnr-MD237 Nnf1bGD13477 metaphase Nnf1bdsRNA.cPa
Classical Alleles ( 0 )
For All Classical Alleles Show
Allele of Nnf1b Class Mutagen Stocks Known lesion
Alleles Carried on Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Nnf1b Class Mutagen Stocks Known lesion Nnf1bGD13477 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Nnf1bAct5C.C.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Nnf1bAct5C.N.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Nnf1bdsRNA.cPa in vitro construct - RNAi 0 Yes Nnf1bScer\UAS.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2L)Exel6002 (Ryder, 2004.4.26) Df(2L)Exel7005 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD13477} NA
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 5 unique terms )
	There is no information supporting a molecular function term for this gene. (Based on a review of available data before: 2006)
Terms Based on Experimental Evidence ( 4 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
chromosome segregation inferred from genetic interaction with Nnf1a (Przewloka et al., 2007) mitotic metaphase plate congression inferred from genetic interaction with Nnf1a (Przewloka et al., 2007)
Cellular Component
CV term Evidence References
kinetochore inferred from direct assay (Przewloka et al., 2007, Schittenhelm et al., 2007) Ndc80 complex inferred from direct assay (Schittenhelm et al., 2007)
Terms Based on Predictions or Assertions ( 1 term )
Molecular Function ( 0 terms)
Biological Process ( 0 terms)
Cellular Component
CV term Evidence References
NMS complex inferred from sequence or structural similarity with human PMF1 (Przewloka et al., 2007)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Nnf1b allele Gene References
External Data
Linkouts	DPiM - DPiM, Drosophila Protein interaction Map • FBgn0051658 DroID - A comprehensive database of gene and protein interactions. • FBgn0051658
Orthologs
Genome-wide drosophilid orthologs	Dere\GG24745 Dsec\GM16767 Dyak\GE17078
Curated drosophilid orthologs	Dere\Nnf1b (Schittenhelm et al., 2007) Dsec\Nnf1b (Schittenhelm et al., 2007) Dsim\Nnf1R-1 (Przewloka et al., 2007) Dsim\Nnf1b (Schittenhelm et al., 2007) Dyak\Nnf1R-1 (Przewloka et al., 2007) Dyak\Nnf1b (Schittenhelm et al., 2007)
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • CPIJ003660 Culex pipens (mosquito) 51856 Trichoplax adhaerens (metazoa) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • FBgn0234561 FBgn0194437 FBgn0171680 FBgn0116876
Stocks & Reagents
Stocks Listed in FlyBase ( 2 )
VDRC	v28788 w1118; P{GD13477}v28788/TM3 v28789 w1118; P{GD13477}v28789/CyO
Genomic Clones ( 2 )
	BACR16I01 BACR30G21 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 4 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE44027
Other clones	DPiM_RE44027
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	RH47570
Other clones	Dmel_neb_007040_0074_2792
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0051658 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 318874
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Nnf1b CG31658 (Schittenhelm et al., 2007)
Source for database merge of
Additional comments
Other Comments
	New annotation (CG31658) in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_164388 RefSeq (Proteins) • NP_722652 DDBJ / EMBL / GenBank • AAN10485 Entrez Gene - A searchable database of RefSeq genes. • 318874 UniProtKB/TrEMBL • Q8IPW3
Other Crossreferences
Linkouts
	DPiM - DPiM, Drosophila Protein interaction Map • FBgn0051658 DroID - A comprehensive database of gene and protein interactions. • FBgn0051658 DRSC - Results from RNAi screens. • FBgn0051658 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0051658 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG31658-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0051658 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 318874 InParanoid A subset of ortholog calls from InParanoid. • CPIJ003660 Culex pipens (mosquito) 51856 Trichoplax adhaerens (metazoa) modMine - Data generated by the modENCODE project. • FBgn0051658 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • FBgn0234561 FBgn0194437 FBgn0171680 FBgn0116876
Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	CG31658 (Schittenhelm et al., 2007, Przewloka et al., 2007) dmNnf1R-1 (Przewloka et al., 2007) Nnf1b (Schittenhelm et al., 2007)
Name Synonym	CG31658   Nnf1b   Nnf1-related protein 1 (Przewloka et al., 2007)
Secondary FlyBase IDs
References ( 8 )
Research paper	Przewloka et al., 2007, PLoS ONE 2(5): e478 Molecular analysis of core kinetochore composition and assembly in Drosophila melanogaster. [FBrf0200394] Schittenhelm et al., 2007, Chromosoma 116(4): 385--402 Spatial organization of a ubiquitous eukaryotic kinetochore protein network in Drosophila chromosomes. [FBrf0200315]
Supplementary material	The modENCODE Consortium, 2010, Science 330(6012): DataS14: Gene co-expression clusters. [FBrf0213979] Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]
Personal communication to FlyBase	Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327] Ryder, 2004.4.26, Exelixis CG deletion data. Exelixis CG deletion data. [FBrf0184335]
FlyBase analysis	FlyBase Genome Annotators, 2002-2003, Changes affecting gene model number or type in releases 3.0 and 3.1 of the annotated D.melanogaster genome. Changes affecting gene model number or type in releases 3.0 and 3.1 of the annotated D.melanogaster genome. [FBrf0148886] FlyBase Curators, 2002-, Use of the ND evidence code for Gene Ontology (GO) terms in FlyBase. Use of the ND evidence code for Gene Ontology (GO) terms in FlyBase. [FBrf0159398]