FB2020_03, released Jun 16, 2020

Gene: Dmel\AspRS-m

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General Information
Symbol
Dmel\AspRS-m
Species
D. melanogaster
Name
Aspartyl-tRNA synthetase, mitochondrial
Annotation Symbol
CG31739
Feature Type
protein_coding_gene
FlyBase ID
FBgn0051739
Gene Model Status
Current
Stock Availability
5 publicly available
Enzyme Name (EC)
Aspartate--tRNA ligase (6.1.1.12)
Gene Snapshot
Aspartyl-tRNA synthetase, mitochondrial (AspRS-m) encodes a protein predicted to function within mitochondria to catalyze the ligation of aspartate to its cognate tRNA to form aspartyl-tRNA. [Date last reviewed: 2019-03-07]
Other Summaries
Key Links
Genomic Location
Cytogenetic map
36B2-36B2
Sequence location
2L:16,827,078..16,832,093 [+]
Recombination map

2-53

RefSeq locus
NT_033779 REGION:16827078..16832093
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (8 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
aminoacyl-tRNA ligase activity
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
aspartate-tRNA ligase activity
traceable author statement
(Lu et al., 2015)
ATP binding
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
nucleic acid binding
inferred from electronic annotation with InterPro:IPR004365
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
Biological Process (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
aspartyl-tRNA aminoacylation
traceable author statement
(Lu et al., 2015)
tRNA aminoacylation for protein translation
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
cytoplasm
inferred from electronic annotation with InterPro:IPR004115
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
mitochondrion
traceable author statement
(Lu et al., 2015)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Catalytic Activity (EC)
Experimental Evidence
-
Predictions / Assertions
ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp) (6.1.1.12)
Summaries
Gene Group (FlyBase)
MITOCHONDRIAL AMINOACYL-TRNA SYNTHETASES -
Mitochondrial aminoacyl-tRNA synthetases catalyze the ligation of amino acids to their cognate tRNAs in mitochondria. They are encoded by nuclear genes and are imported into mitochondria with the guidance of a 'mitochondrial targeting sequence'. (Adapted from FBrf0230668.)
Gene Model and Products
Number of Transcripts
3
Number of Unique Polypeptides
2

Please see the GBrowse view of Dmel\AspRS-m or the JBrowse view of Dmel\AspRS-m for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.45

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 6.32

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
AspRS-m-RA
FBtr0080919
NM_165196
3899
1082
AspRS-m-RB
FBtr0331622
NM_001273590
4153
1082
AspRS-m-RD
FBtr0479831
NM_001273591
3865
1139
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
AspRS-m-PA
FBpp0080475
121.5
1082
5.57
NP_724018
AAF53577
AspRS-m-PB
FBpp0304012
121.5
1082
5.57
NP_001260519
AGB93054
AspRS-m-PD
FBpp0428165
128.0
1139
6.08
AGB93055
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1082 aa isoforms: AspRS-m-PA, AspRS-m-PB
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\AspRS-m using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\AspRS-m in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 3 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of AspRS-m
Transgenic constructs containing regulatory region of AspRS-m
Deletions and Duplications ( 3 )
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\DARS2
14 of 15
Yes
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Dars2
13 of 15
Yes
Yes
Rattus norvegicus (Norway rat) (1)
Rnor\Dars2
12 of 13
Yes
Yes
Xenopus tropicalis (Western clawed frog) (2)
Xtro\dars2
8 of 12
Yes
Yes
Xtro\LOC101734189
1 of 12
No
Yes
Danio rerio (Zebrafish) (1)
Drer\dars2
13 of 15
Yes
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\dars-2
12 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (2)
Atha\AT4G33760
7 of 9
Yes
Yes
Atha\AT3G30770
1 of 9
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\MSD1
13 of 15
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\SPCC736.06
10 of 12
Yes
Yes
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091901UR )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00000514
Drosophila simulans
Dsim\GD24088
Drosophila sechellia
Dsec\GM17213
Drosophila erecta
Dere\GG22777
Drosophila yakuba
Dyak\GE12772
Drosophila ananassae
Dana\GF15370
Drosophila pseudoobscura pseudoobscura
Dpse\GA16436
Drosophila persimilis
Dper\GL18794
Drosophila willistoni
Dwil\GK18240
Drosophila virilis
Dvir\GJ17510
Drosophila mojavensis
Dmoj\GI14195
Drosophila grimshawi
Dgri\GH13102
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091501HO )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17026306
Glossina morsitans
Tsetse fly
Gmor\GMOY010037
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_07185
Mayetiola destructor
Hessian fly
Mdes\Mdes018869
Mayetiola destructor
Hessian fly
Mdes\Mdes012753
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL001200
Anopheles darlingi
American malaria mosquito
Adar\ADAC007063
Anopheles gambiae
Malaria mosquito
Agam\AGAP007844
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ005915
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W01AE )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA010863
Bombyx mori
Silkmoth
Bmor\BGIBMGA010864
Danaus plexippus
Monarch butterfly
Dple\DPOGS211403-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL009858
Apis florea
Little honeybee
Aflo\2147623043
Apis mellifera
Western honey bee
Amel\LOC725136
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP13357
Bombus terrestris
Buff-tailed bumblebee
Bter\15206525
Linepithema humile
Argentine ant
Lhum\LH14923
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:001ace
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG010510
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_00274
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_03223
Tribolium castaneum
Red flour beetle
Tcas\TC013060
Pediculus humanus
Human body louse
Phum\PHUM137290
Rhodnius prolixus
Kissing bug
Rpro\RPRC012018
Cimex lectularius
Bed bug
Clec\CLEC007027
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI008435
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_04150
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X03YJ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR007313
Ixodes scapularis
Black-legged tick
Isca\ISCW013693
Stegodyphus mimosarum
African social velvet spider
Smim\KFM69657.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur35g01480
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_264578
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G04HW )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Asps
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Dars2
Ciona intestinalis
Vase tunicate
Cint\LOC100187179
Gallus gallus
Domestic chicken
Ggal\LOC771323
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (4)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
AsnRS
2 of 10
AsnRS-m
2 of 10
AspRS
1 of 10
LysRS
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    DARS2; aspartyl-tRNA synthetase 2, mitochondrial
    14 of 15
    610956
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      esyN Network Diagram
      Show neighbor-neighbor interactions:
      Select Layout:
      Legend:
      Protein
      RNA
      Selected Interactor(s)
      Interactions Browser
      View in Interactions Browser
      Please see the Physical Interaction reports below for full details
      protein-protein
      Physical Interaction
      Assay
      References
      AspRS-m - Dsor1
      anti tag coimmunoprecipitation, peptide massfingerprinting
      (Friedman et al., 2011)
      AspRS-m - Pten
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      AspRS-m - Raf
      anti tag coimmunoprecipitation, peptide massfingerprinting
      (Friedman et al., 2011)
      Summary of Genetic Interactions
      esyN Network Diagram
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      External Data
      Linkouts
      DroID - A comprehensive database of gene and protein interactions.
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Pathways
      Signaling Pathways (FlyBase)
      Metabolic Pathways
      FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel
      KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
      External Data
      Linkouts
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      2L
      Recombination map

      2-53

      Cytogenetic map
      36B2-36B2
      Sequence location
      2L:16,827,078..16,832,093 [+]
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      36B2-36B2
      Limits computationally determined from genome sequence between P{lacW}Mhck10423&P{lacW}Cask03902 and P{lacW}Aac11k06710
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      Experimentally Determined Recombination Data
      Location

      2-53

      (FlyBase, 2016)

      2-51.3

      (Comeron et al., 2012)
      Left of (cM)
      Right of (cM)
      Notes
      Stocks and Reagents
      Stocks (5)
      Genomic Clones (16)
       

      Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

      cDNA Clones (48)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequenced
      BDGP DGC clones
      Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        Antibody Information
        Laboratory Generated Antibodies
         
        Commercially Available Antibodies
         
        Other Information
        Relationship to Other Genes
        Source for database identify of

        Source for identity of: AspRS-m CG31739

        (Lu et al., 2015)
        Source for database merge of
        Additional comments

        Release 2 annotation CG17938 split into CG31739 and CG31991 (mdy) in release 3 of the genome annotation. In addition, CG31991 contains sequences corresponding to release 2 annotation CG13273.

        (FlyBase Genome Annotators, 2002-2003)
        Other Comments
        Origin and Etymology
        Discoverer
        Etymology
        Identification
        External Crossreferences and Linkouts ( 43 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
        Other crossreferences
        BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
        Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
        InterPro - A database of protein families, domains and functional sites
        KEGG Genes - Molecular building blocks of life in the genomic space.
        modMine - A data warehouse for the modENCODE project
        Linkouts
        DroID - A comprehensive database of gene and protein interactions.
        DRSC - Results frm RNAi screens
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
        FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel
        FlyMine - An integrated database for Drosophila genomics
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Synonyms and Secondary IDs (4)
        Datasets (0)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        References (39)