FlyBase Gene Report: Dmel\ZnT33D

General Information

Symbol

Dmel\ZnT33D

Species

D. melanogaster

Name

Zinc transporter 33D

Annotation Symbol

CG31860

Feature Type

protein_coding_gene

FlyBase ID

FBgn0051860

Gene Model Status

Current

Stock Availability

5 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

dZnT33D

Key Links

Genomic Location

Cytogenetic map

33D2-33D2

Sequence location

2L:12,164,241..12,166,704 [+]

Recombination map

2-46

RefSeq locus

NT_033779 REGION:12164241..12166704

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (10 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables zinc ion transmembrane transporter activity

inferred by curator from GO:0016323

(Lye et al., 2013)

inferred from sequence or structural similarity with UniProtKB:Q62941

(FlyBase Curators, 2008-)

inferred from biological aspect of ancestor with PANTHER:PTN000169409

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (6 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

involved_in cellular zinc ion homeostasis

inferred from mutant phenotype

(Qin et al., 2013)

involved_in detoxification of zinc ion

inferred from mutant phenotype

(Lye et al., 2013)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

involved_in regulation of sequestering of zinc ion

inferred from biological aspect of ancestor with PANTHER:PTN000169310

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in response to zinc ion

inferred from biological aspect of ancestor with PANTHER:PTN000169310

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in zinc ion transmembrane transport

inferred from biological aspect of ancestor with PANTHER:PTN000169409

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in zinc ion transport

inferred from sequence or structural similarity with UniProtKB:Q62941

(FlyBase Curators, 2008-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in basolateral plasma membrane

inferred from direct assay

(Lye et al., 2013)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

located_in integral component of membrane

inferred from electronic annotation with InterPro:IPR002524

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

is_active_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN000169409

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

SLC30 FAMILY OF ZINC TRANSPORTERS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

SLC30 FAMILY OF ZINC TRANSPORTERS -: Solute carrier family 30 (SLC30) or Zinc Transporters (ZnT) proteins transport Zn²⁺ from the cytosol across the plasma membrane or organelle membranes allowing accumulation into intracellular compartments. (Adapted from FBrf0220942).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\ZnT33D for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VKA3)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.45

Alternative translation stop created by use of multiphasic reading frames within coding region.

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

ZnT33D-RB

FBtr0302984

NM_164998

2257

701

ZnT33D-RC

FBtr0302985

NM_001201864

2264

436

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

ZnT33D-PB

FBpp0292109

78.1

701

7.23

NP_723732

AAF53175

ZnT33D-PC

FBpp0292110

48.7

436

7.43

NP_001188793

ADV37043

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ZnT33D using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

ZnT33D_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in basolateral plasma membrane

inferred from direct assay

(Lye et al., 2013)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\ZnT33D in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\ZnT33D

View exonic expression by tissue for Dmel\ZnT33D

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0051860

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

326167/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0051860

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0051860

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0051860

Flygut - An atlas of the Drosophila adult midgut

FBgn0051860

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 1 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 8 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of ZnT33D

Transgenic constructs containing regulatory region of ZnT33D

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(2L)Exel6032

(Ryder, 2004.4.26)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^pnr-MD237

lethal - all die during larval stage, with Scer\GAL4^Mef2.PR

ZnT33D^GD1098

lethal - all die during larval stage, with Scer\GAL4^Tub.PU

ZnT33D^VDRC.cUa

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

ZnT33D^GD1098

viable, with Scer\GAL4^tin.CΔ4

ZnT33D^GD1098

Other Phenotypes

Allele

abnormal body color, with Scer\GAL4^pnr-MD237

ZnT33D^VDRC.cUa

some die during pupal stage, with Scer\GAL4^pnr-MD237

ZnT33D^VDRC.cUa

visible, with Scer\GAL4^GMR.PFa

ZnT33D^VDRC.cUa

visible, with Scer\GAL4^pnr-MD237

visible, with Scer\GAL4^Tub.PU

ZnT33D^{UAS.PB.Tag:FLAG}

Phenotype manifest in

Allele

adult thorax, with Scer\GAL4^pnr-MD237

ZnT33D^VDRC.cUa

eye, with Scer\GAL4^GMR.PFa

ZnT33D^VDRC.cUa

humeral bristle, with Scer\GAL4^pnr-MD237

ZnT33D^{UAS.PB.Tag:FLAG}

humeral bristle, with Scer\GAL4^Tub.PU

ZnT33D^{UAS.PB.Tag:FLAG}

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (9)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\SLC30A2

10 of 15

Yes

Hsap\SLC30A3

9 of 15

Yes

Hsap\SLC30A8

8 of 15

Yes

3 of 15

1 of 15

1 of 15

1 of 15

Yes

Hsap\SLC30A7

1 of 15

Hsap\SLC30A10

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (9)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Slc30a2

9 of 15

Yes

Mmus\Slc30a3

9 of 15

Yes

Mmus\Slc30a8

8 of 15

Yes

2 of 15

1 of 15

1 of 15

1 of 15

Yes

Mmus\Slc30a7

1 of 15

Mmus\Slc30a10

1 of 15

Rattus norvegicus (Norway rat) (9)

Rnor\Slc30a3

10 of 13

Yes

Rnor\Slc30a8

8 of 13

Yes

7 of 13

3 of 13

1 of 13

1 of 13

1 of 13

Yes

Rnor\Slc30a7

1 of 13

Rnor\Slc30a10

1 of 13

Xenopus tropicalis (Western clawed frog) (9)

Xtro\slc30a2

9 of 12

Yes

Xtro\slc30a8

8 of 12

Yes

Xtro\slc30a3

6 of 12

Yes

3 of 12

1 of 12

1 of 12

1 of 12

Yes

Xtro\slc30a7

1 of 12

Xtro\slc30a10

1 of 12

Danio rerio (Zebrafish) (9)

Drer\slc30a2

8 of 15

Yes

6 of 15

3 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Drer\slc30a7

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (5)

Cele\cdf-2

8 of 15

Yes

Cele\ttm-1

3 of 15

Cele\cdf-1

1 of 15

Cele\toc-1

1 of 15

Yes

Cele\Y105E8A.3

1 of 15

Arabidopsis thaliana (thale-cress) (4)

Atha\MTPA1

7 of 9

Yes

Atha\MTPA2

6 of 9

Atha\MTPB1

6 of 9

Atha\ZAT

6 of 9

Saccharomyces cerevisiae (Brewer's yeast) (5)

Scer\COT1

1 of 15

Yes

Scer\MMT1

1 of 15

Yes

Scer\MMT2

1 of 15

Yes

Scer\MSC2

1 of 15

Yes

Scer\ZRC1

1 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (5)

Gene Symbol

Score

Source

Alignment

6 of 10

4 of 10

2 of 10

2 of 10

2 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

SLC30A8; solute carrier family 30 member 8

model of type 2 diabetes mellitus

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

SLC30A2; solute carrier family 30 member 2

10 of 15

609617

ZINC DEFICIENCY, TRANSIENT NEONATAL; TNZD

SLC30A3; solute carrier family 30 member 3

9 of 15

602878

SLC30A8; solute carrier family 30 member 8

8 of 15

611145

TYPE 2 DIABETES MELLITUS; T2D

type 2 diabetes mellitus

SLC30A4; solute carrier family 30 member 4

3 of 15

602095

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Lye et al., 2013)

enhanceable

(Dechen et al., 2015)

Zip71B

suppressible

ZnT33D

(Dechen et al., 2015)

ZnT86D

enhanceable

ZnT33D

(Dechen et al., 2015)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0051860

MIST (genetic) - An integrated Molecular Interaction Database

ZnT33D

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-46

Cytogenetic map

33D2-33D2

Sequence location

2L:12,164,241..12,166,704 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

33D2-33D2

Limits computationally determined from genome sequence between P{PZ}Rab6⁰⁸³²³ and P{EP}Elf^EP476&P{EP}bun^EP488

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-46

(FlyBase, 2016)

2-39.7

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (5)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

26425

w¹¹¹⁸; Mi{ET1}CG31760^MB08990 ZnT33D^MB08990

68011

y¹ sc^* v¹ sev²¹; P{TOE.GS00255}attP40

38312

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01776}attP40

VDRC

v103398

P{KK100432}VIE-260B

v7688

w¹¹¹⁸; P{GD1098}v7688

Genomic Clones (12)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (5)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0051860

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0051860

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

326167

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: ZnT33D CG31860

(Lye et al., 2012)

Source for database merge of

Additional comments

Annotation CG17215 split into CG31860, CG31861 and CG31760 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Other Comments

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 33 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

326167

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VKA3

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VKA3

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0051860

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0051860

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

326167/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0051860

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

ZnT33D_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0051860

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0051860

Flygut - An atlas of the Drosophila adult midgut

FBgn0051860

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

326167

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG31860

MARRVEL_MODEL - MARRVEL (model organism gene)

326167

modMine - A data warehouse for the modENCODE project

FBgn0051860

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0051860

DRSC - Results frm RNAi screens

FBgn0051860

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0051860

FlyMine - An integrated database for Drosophila genomics

FBgn0051860

MIST (genetic) - An integrated Molecular Interaction Database

ZnT33D

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

CG17215

(Brody and Cravchik, 2000)

CG31860

(Yin et al., 2017, Richards and Burke, 2016, Xiao and Zhou, 2016, Hosono et al., 2015, Qin et al., 2013, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Wang et al., 2009)

ZNT33D

(Richards and Burke, 2016)

ZnT33D

(Richards et al., 2017, Transgenic RNAi Project members, 2017-)

cg31860

(Lye et al., 2012)

dZnT33D

(Calap-Quintana et al., 2017, Yin et al., 2017, Xiao and Zhou, 2016, Dechen et al., 2015, Lye et al., 2013, Lye et al., 2012, Lye et al., 2012)

Name Synonyms

Zinc transporter 33D

Secondary FlyBase IDs