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Gene Dmel\CG32376

General Information
Symbol	Dmel\CG32376	Species	D. melanogaster
Name		Annotation symbol	CG32376
Feature type	protein_coding_gene	FlyBase ID	FBgn0052376
Gene Model Status	Current	Stock availability	4 publicly available
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	66A5-66A5	Sequence location	3L:7,534,682..7,535,557 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	This gene is referred to in FlyBase by the symbol Dmel\CG32376 (FBgn0052376). It is a protein_coding_gene from Drosophila melanogaster. An electronic pipeline based on InterPro domains suggests that it has the molecular function: serine-type endopeptidase activity. An electronic pipeline based on InterPro domains suggests that it is involved in the biological process: proteolysis. 3 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Peptidase S1; Peptidase S1, trypsin family, active site; Peptidase S1A, chymotrypsin-type; Trypsin-like cysteine/serine peptidase domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very low expression to a trough of no expression detected. Peak expression observed during early larval stages. Summary of FlyAtlas Anatomical Expression Data: No expression detected in any larval or adult organs/tissues. Comments on Affy2 ProbeSet: ProbeSet 1638532_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of CG32376. Gene sequence location is 3L:7534682..7535557.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 66A5-66A5   Limits computationally determined from genome sequence between P{PZ}l(3)0721708223&P{EP}Rac2EP3118 and P{lacW}Nmtj1C7
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\CG32376 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.45
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) CG32376-RA FBtr0076827  NM_168223   876   291
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein CG32376-PA   FBpp0076538   33.1   291   10.13     NP_729271   AAN12043
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Peptidase S1 (IPR001254) Peptidase S1A, chymotrypsin-type (IPR001314) Trypsin-like cysteine/serine peptidase domain (IPR009003) Peptidase S1, trypsin family, active site (IPR018114)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014296 AAN12043
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q8IQ89
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: No expression detected in any larval or adult organs/tissues. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    2.325 Larval Midgut    3.3 Larval Hindgut    2.9 Larval Malpighian Tubules    2.5 Larval Fat Body    4.8 Larval Salivary Gland    3.8 Larval Trachea    3.7 Larval Carcass    6.875 Adult Head    2.1 Adult Eye    3.875 Adult Brain    1.4 Adult Thoracic-Abdominal Ganglion    0.8 Adult Crop    1.4 Adult Midgut    9.4 Adult Hindgut    1.6 Adult Malpighian Tubules    3.3 Adult Fat Body    4.1 Adult Salivary Gland    3.3 Adult Heart    1.475 Adult VirginFemale Spermatheca    3.3 Adult InseminatedFemale Spermatheca    1.8 Adult Ovary    1.8 Adult Testis    1.4 Adult Male Accessory Gland    4.5 Adult Carcass    3.7 Expression Level Scale  None   Low  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    2.325 Larval Midgut    3.3 Larval Hindgut    2.9 Larval Malpighian Tubules    2.5 Larval Fat Body    4.8 Larval Salivary Gland    3.8 Larval Trachea    3.7 Larval Carcass    6.875 Adult Head    2.1 Adult Eye    3.875 Adult Brain    1.4 Adult Thoracic-Abdominal Ganglion    0.8 Adult Crop    1.4 Adult Midgut    9.4 Adult Hindgut    1.6 Adult Malpighian Tubules    3.3 Adult Fat Body    4.1 Adult Salivary Gland    3.3 Adult Heart    1.475 Adult VirginFemale Spermatheca    3.3 Adult InseminatedFemale Spermatheca    1.8 Adult Ovary    1.8 Adult Testis    1.4 Adult Male Accessory Gland    4.5 Adult Carcass    3.7 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    2.325 Larval Midgut    3.3 Larval Hindgut    2.9 Larval Malpighian Tubules    2.5 Larval Fat Body    4.8 Larval Salivary Gland    3.8 Larval Trachea    3.7 Larval Carcass    6.875 Adult Head    2.1 Adult Eye    3.875 Adult Brain    1.4 Adult Thoracic-Abdominal Ganglion    0.8 Adult Crop    1.4 Adult Midgut    9.4 Adult Hindgut    1.6 Adult Malpighian Tubules    3.3 Adult Fat Body    4.1 Adult Salivary Gland    3.3 Adult Heart    1.475 Adult VirginFemale Spermatheca    3.3 Adult InseminatedFemale Spermatheca    1.8 Adult Ovary    1.8 Adult Testis    1.4 Adult Male Accessory Gland    4.5 Adult Carcass    3.7 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    2.325 Larval Midgut    3.3 Larval Hindgut    2.9 Larval Malpighian Tubules    2.5 Larval Fat Body    4.8 Larval Salivary Gland    3.8 Larval Trachea    3.7 Larval Carcass    6.875 Adult Head    2.1 Adult Eye    3.875 Adult Brain    1.4 Adult Thoracic-Abdominal Ganglion    0.8 Adult Crop    1.4 Adult Midgut    9.4 Adult Hindgut    1.6 Adult Malpighian Tubules    3.3 Adult Fat Body    4.1 Adult Salivary Gland    3.3 Adult Heart    1.475 Adult VirginFemale Spermatheca    3.3 Adult InseminatedFemale Spermatheca    1.8 Adult Ovary    1.8 Adult Testis    1.4 Adult Male Accessory Gland    4.5 Adult Carcass    3.7 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    2.325 Larval Midgut    3.3 Larval Hindgut    2.9 Larval Malpighian Tubules    2.5 Larval Fat Body    4.8 Larval Salivary Gland    3.8 Larval Trachea    3.7 Larval Carcass    6.875 Adult Head    2.1 Adult Eye    3.875 Adult Brain    1.4 Adult Thoracic-Abdominal Ganglion    0.8 Adult Crop    1.4 Adult Midgut    9.4 Adult Hindgut    1.6 Adult Malpighian Tubules    3.3 Adult Fat Body    4.1 Adult Salivary Gland    3.3 Adult Heart    1.475 Adult VirginFemale Spermatheca    3.3 Adult InseminatedFemale Spermatheca    1.8 Adult Ovary    1.8 Adult Testis    1.4 Adult Male Accessory Gland    4.5 Adult Carcass    3.7 Expression Level Scale  None   Low  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    2.325 Larval Midgut    3.3 Larval Hindgut    2.9 Larval Malpighian Tubules    2.5 Larval Fat Body    4.8 Larval Salivary Gland    3.8 Larval Trachea    3.7 Larval Carcass    6.875 Adult Head    2.1 Adult Eye    3.875 Adult Brain    1.4 Adult Thoracic-Abdominal Ganglion    0.8 Adult Crop    1.4 Adult Midgut    9.4 Adult Hindgut    1.6 Adult Malpighian Tubules    3.3 Adult Fat Body    4.1 Adult Salivary Gland    3.3 Adult Heart    1.475 Adult VirginFemale Spermatheca    3.3 Adult InseminatedFemale Spermatheca    1.8 Adult Ovary    1.8 Adult Testis    1.4 Adult Male Accessory Gland    4.5 Adult Carcass    3.7 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    2.325 Larval Midgut    3.3 Larval Hindgut    2.9 Larval Malpighian Tubules    2.5 Larval Fat Body    4.8 Larval Salivary Gland    3.8 Larval Trachea    3.7 Larval Carcass    6.875 Adult Head    2.1 Adult Eye    3.875 Adult Brain    1.4 Adult Thoracic-Abdominal Ganglion    0.8 Adult Crop    1.4 Adult Midgut    9.4 Adult Hindgut    1.6 Adult Malpighian Tubules    3.3 Adult Fat Body    4.1 Adult Salivary Gland    3.3 Adult Heart    1.475 Adult VirginFemale Spermatheca    3.3 Adult InseminatedFemale Spermatheca    1.8 Adult Ovary    1.8 Adult Testis    1.4 Adult Male Accessory Gland    4.5 Adult Carcass    3.7 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    2.325 Larval Midgut    3.3 Larval Hindgut    2.9 Larval Malpighian Tubules    2.5 Larval Fat Body    4.8 Larval Salivary Gland    3.8 Larval Trachea    3.7 Larval Carcass    6.875 Adult Head    2.1 Adult Eye    3.875 Adult Brain    1.4 Adult Thoracic-Abdominal Ganglion    0.8 Adult Crop    1.4 Adult Midgut    9.4 Adult Hindgut    1.6 Adult Malpighian Tubules    3.3 Adult Fat Body    4.1 Adult Salivary Gland    3.3 Adult Heart    1.475 Adult VirginFemale Spermatheca    3.3 Adult InseminatedFemale Spermatheca    1.8 Adult Ovary    1.8 Adult Testis    1.4 Adult Male Accessory Gland    4.5 Adult Carcass    3.7 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    2.1   NA  Eye    3.875   NA  Brain    1.4   2.325  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    0.8   NA  Crop    1.4   3.3  Midgut    9.4   2.9  Hindgut    1.6   2.5  Malpighian Tubules    3.3   4.8  Fat Body    4.1   3.8  Salivary Gland    3.3   NA  Heart    1.475   3.7  Trachea    NA   NA  VirginFemale Spermatheca    3.3   NA  InseminatedFemale Spermatheca    1.8   NA  Ovary    1.8   NA  Testis    1.4   NA  Male Accessory Gland    4.5   6.875  Carcass    3.7 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very low expression to a trough of no expression detected. Peak expression observed during early larval stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    1 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    1 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    1 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    1 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    1 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    1 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    1 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    1 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Treatments RNA-Seq
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (Graveley et al., 2011) mE1_20_mRNA_expression_cluster_08
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0052376 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG32376-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable CG32376f01583 viable, with Scer\GAL4pnr-MD237 CG32376GD10870 Sterility Allele fertile CG32376f01583 Phenotype manifest in Allele
Classical Alleles ( 1 )
For All Classical Alleles Show
Allele of CG32376 Class Mutagen Stocks Known lesion CG32376f01583 piggyBac transposase 2 --
Alleles Carried on Transgenic Constructs ( 2 )
For All Alleles Carried on Transgenic Constructs Show
Allele of CG32376 Class Mutagen Stocks Known lesion CG32376GD10870 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes CG32376KK110233 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD10870} NA P{KK110233} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{WH}CG32376f01583 NA
Gene Ontology: Function, Process & Cellular Component ( 2 unique terms )
Terms Based on Experimental Evidence ( 0 terms )
Terms Based on Predictions or Assertions ( 2 terms )
Molecular Function
CV term Evidence References
serine-type endopeptidase activity inferred from electronic annotation with InterPro:IPR001254, InterPro:IPR001314, InterPro:IPR018114 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
proteolysis inferred from electronic annotation with InterPro:IPR001254, InterPro:IPR001314, InterPro:IPR018114 (FlyBase Curators et al., 2004-)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions CG32376 allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0052376
Orthologs
OrthoDB Orthologs (14) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG618C7G Dipteran inclusive ortholog search No orthologs identified Insect inclusive ortholog search No orthologs identified Arthropod inclusive ortholog search No orthologs identified Metazoa inclusive ortholog search No orthologs identified
Orthologs in Drosophila Species (EOG618C7G)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\CG32376     Drosophila melanogaster fruit fly  Dmel\CG32374     Drosophila simulans   Dsim\GD13048   Y Drosophila sechellia   Dsec\GM13747   Y Drosophila erecta   Dere\GG14952   Y Drosophila yakuba   Dyak\GE20403   Y Drosophila yakuba   Dyak\GE20402 Y Y Drosophila ananassae   Dana\GF23780 Y Y Drosophila ananassae   Dana\GF23781   Y Drosophila pseudoobscura pseudoobscura   Dpse\GA16851   Y Drosophila persimilis   Dper\GL24175   Y Drosophila willistoni   Dwil\GK19265   Y Drosophila virilis   Dvir\GJ12353   Y Drosophila grimshawi   Dgri\GH16608   Y
Orthologs in non-Drosophila Dipterans (None identified)
No non-Drosophilid orthologies identified
Orthologs in non-Dipteran Insects (None identified)
No non-Dipteran orthologies identified
Orthologs in non-Insect Arthropods (None identified)
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (2) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila yakuba Dyak\GE20402 Drosophila ananassae Dana\GF23780
Stocks & Reagents
Stocks Listed in FlyBase ( 4 )
Bloomington	18450 w1118; PBac{WH}CG32376f01583
Harvard	- PBac{WH}CG32376f01583
VDRC	v101917 P{KK110233}VIE-260B v41885 w1118; P{GD10870}v41885/TM3
Genomic Clones ( 1 )
	BACR10H23 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 0 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0052376 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 318002
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments	Annotation CG8528 split into CG32374 and CG32376 in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003)
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_168223 RefSeq (Proteins) • NP_729271 DDBJ / EMBL / GenBank • AAN12043 Entrez Gene - A searchable database of RefSeq genes. • 318002 UniProtKB/TrEMBL • Q8IQ89
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Peptidase S1 (IPR001254) Peptidase S1A, chymotrypsin-type (IPR001314) Trypsin-like cysteine/serine peptidase domain (IPR009003) Peptidase S1, trypsin family, active site (IPR018114)
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0052376 DRSC - Results from RNAi screens. • FBgn0052376 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0052376 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG32376-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0052376 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 318002 modMine - Data generated by the modENCODE project. • FBgn0052376
Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	CG8528   CG32376 (Keleman et al., 2009.8.5, Chen et al., 2010) SP148 (Ross et al., 2003)
Name Synonym
Secondary FlyBase IDs
	FBgn0035796
References ( 12 )
Research paper	Ross et al., 2003, Gene 304(1-2): 117--131 Serine proteases and their homologs in the Drosophila melanogaster genome: an initial analysis of sequence conservation and phylogenetic relationships. [FBrf0155784]
Supplementary material	Graveley et al., 2011, Nature 471(7339): Supplementary Table 33. Fuzzy Cluster Members. [FBrf0213980] Chen et al., 2010, Science 330(6011): Supporting Online Material. [FBrf0216693] Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]
Personal communication to FlyBase	Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510] Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327] Bloomington Drosophila Stock Center, 2004.11.6, Exelixis insertion alleles that are not in FlyBase. Exelixis insertion alleles that are not in FlyBase. [FBrf0179797] FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215]
FlyBase analysis	FlyBase Genome Annotators, 2002-2003, Changes affecting gene model number or type in releases 3.0 and 3.1 of the annotated D.melanogaster genome. Changes affecting gene model number or type in releases 3.0 and 3.1 of the annotated D.melanogaster genome. [FBrf0148886]
Curated genome annotation	FamiliarityBreedsContempt, 1999.11, [Automatic annotations performed at the Celera Jamboreee.] [Automatic annotations performed at the Celera Jamboreee.] [FBrf0126705] Gong, 1999.11, [Annotations performed at the Celera Jamboreee.] [Annotations performed at the Celera Jamboreee.] [FBrf0126669]