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Gene Dmel\CG32486

General Information
Symbol	Dmel\CG32486	Species	D. melanogaster
Name		Annotation symbol	CG32486
Feature type	protein_coding_gene	FlyBase ID	FBgn0052486
Gene Model Status	Current	Stock availability	4 publicly available
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	63A5-63A6	Sequence location	3L:3,055,904..3,070,839 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	This gene is referred to in FlyBase by the symbol Dmel\CG32486 (FBgn0052486). It is a protein_coding_gene from Drosophila melanogaster. An electronic pipeline based on InterPro domains suggests that it has the molecular function: ubiquitin-protein ligase activity; zinc ion binding. An electronic pipeline based on InterPro domains suggests that it is involved in the biological process: protein ubiquitination. 6 alleles are reported. The phenotypes of these alleles are annotated with: trichogen cell; mesothoracic tergum. It has one annotated transcript and one annotated polypeptide. Protein features are: Seven In Absentia Homolog-type; TRAF-like; Zinc finger, RING-type; Zinc finger, RING/FYVE/PHD-type; Zinc finger, TRAF-type. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderate expression. Peak expression observed within 06-18 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, adult crop, adult Malpighian tubules, adult male accessory gland, adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, larval/adult midgut, larval/adult hindgut, larval Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult testis, larval carcass. Comments on Affy2 ProbeSet: ProbeSet 1624119_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of CG32486. Gene sequence location is 3L:3055904..3070839.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2011_10
FB2012_01	Sequence features BKN30743 HFA08488 HFA08413
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 63A5-63A6   Limits computationally determined from genome sequence between P{PZ}l(3)0680306803 and P{PZ}kst01318
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\CG32486 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) CG32486-RD FBtr0073004  NM_139508   2360   412
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein CG32486-PD   FBpp0072874   45.4   412   7.65     NP_647765   AAF47711
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Zinc finger, TRAF-type (IPR001293) Zinc finger, RING-type (IPR001841) TRAF-like (IPR008974) Zinc finger, RING/FYVE/PHD-type (IPR013083) Seven In Absentia Homolog-type (IPR013323)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA440953   AA699228   AE014296 AAF47711   AI293335   AI517784   AI518505   AI544175   AW943926   AX094143   AY052026 AAK93450   BF490164   BF497351   BF497380   BF501895   BG633516   BG634944   BG637180   BI163779   BI164358   BI165136   BI166354   BI169571   BI213245   BI215837   BI227491   BI230557   BI231575   BI232433   BI235023   BI235695   BI235928   BI239994   BI243594   BI361456   BI364682   BI368383   BI369733   BI371363   BI374404   BI374731   BI375207   BI487252   BI565790   BI576900   BI582996   BI587094   BI612481   BI619147   BI630141   BP543751   BP549731   BP550167   BP553143   BP557908   CA804703   CA805704   CA806241   CA806248   CO177391   CO177405   CO188794   CO263414   CO264375   CO265620   CO270253   CO275863   CO275883   CO277651   CO277764   CO278374   CO279574   CO281385   CO281898   CO284350   CO286145   CO288224   CO295821   CO301784   CO308868   CO309071   CO311020   CO319779   CO333798   CO340455   CO341976   CO342740   CO342748   CZ471910   CZ471911   EC061327   EC062580   EC065610   EC066293   EC070083   EC073401   EC075300   EC081537   EC084291   EC089440   EC194652   EC195948   EC196926   EC198679   EC201556   EC202158   EC206135   EC206773   EC208237   EC208341   EC209898   EC211172   EC218482   EC221563   EC226680   EC231284   EC231953   EC233180   EC243121   EC252337   EC253043   EC253623   EC255849
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VZV5
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0052486    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0052486 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderate expression. Peak expression observed within 06-18 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0052486 expression level Developmental Stage   Expression Level embryo 00-02hr    627 embryo 02-04hr    2453 embryo 04-06hr    7992 embryo 06-08hr    11344 embryo 08-10hr    11093 embryo 10-12hr    11664 embryo 12-14hr    10679 embryo 14-16hr    4633 embryo 16-18hr    3857 embryo 18-20hr    4007 embryo 20-22hr    2133 embryo 22-24hr    2863 larva L1    1130 larva L2    1028 larva L3 12hr old    1286 larva L3 puffstage 1-2    1367 larva L3 puffstage 3-6    1856 larva L3 puffstage 7-9    2702 white prepupae new    2493 white prepupae 12hr    4488 white prepupae 24hr    4253 pupae 2d postWPP    3644 pupae 3d postWPP    2026 pupae 4d postWPP    1856 adult male 01day    2652 adult male 05day    2654 adult male 30day    2705 adult female 01day    1353 adult female 05day    882 adult female 30day    1001 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (627) embryo 02-04hr  (2453) embryo 04-06hr  (7992) embryo 06-08hr  (11344) embryo 08-10hr  (11093) embryo 10-12hr  (11664) embryo 12-14hr  (10679) embryo 14-16hr  (4633) embryo 16-18hr  (3857) embryo 18-20hr  (4007) embryo 20-22hr  (2133) embryo 22-24hr  (2863) larva L1  (1130) larva L2  (1028) larva L3 12hr old  (1286) larva L3 puffstage 1-2  (1367) larva L3 puffstage 3-6  (1856) larva L3 puffstage 7-9  (2702) white prepupae new  (2493) white prepupae 12hr  (4488) white prepupae 24hr  (4253) pupae 2d postWPP  (3644) pupae 3d postWPP  (2026) pupae 4d postWPP  (1856) adult male 01day  (2652) adult male 05day  (2654) adult male 30day  (2705) adult female 01day  (1353) adult female 05day  (882) adult female 30day  (1001) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    627 embryo 02-04hr  (2453) embryo 04-06hr  (7992) embryo 06-08hr  (11344) embryo 08-10hr  (11093) embryo 10-12hr  (11664) embryo 12-14hr  (10679) embryo 14-16hr  (4633) embryo 16-18hr  (3857) embryo 18-20hr  (4007) embryo 20-22hr  (2133) embryo 22-24hr  (2863) larva L1    1130 larva L2    1028 larva L3 12hr old    1286 larva L3 puffstage 1-2    1367 larva L3 puffstage 3-6  (1856) larva L3 puffstage 7-9  (2702) white prepupae new  (2493) white prepupae 12hr  (4488) white prepupae 24hr  (4253) pupae 2d postWPP  (3644) pupae 3d postWPP  (2026) pupae 4d postWPP  (1856) adult male 01day  (2652) adult male 05day  (2654) adult male 30day  (2705) adult female 01day    1353 adult female 05day    882 adult female 30day    1001 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    627 embryo 02-04hr    2453 embryo 04-06hr    7992 embryo 06-08hr  (11344) embryo 08-10hr  (11093) embryo 10-12hr  (11664) embryo 12-14hr  10679 embryo 14-16hr    4633 embryo 16-18hr    3857 embryo 18-20hr    4007 embryo 20-22hr    2133 embryo 22-24hr    2863 larva L1    1130 larva L2    1028 larva L3 12hr old    1286 larva L3 puffstage 1-2    1367 larva L3 puffstage 3-6    1856 larva L3 puffstage 7-9    2702 white prepupae new    2493 white prepupae 12hr    4488 white prepupae 24hr    4253 pupae 2d postWPP    3644 pupae 3d postWPP    2026 pupae 4d postWPP    1856 adult male 01day    2652 adult male 05day    2654 adult male 30day    2705 adult female 01day    1353 adult female 05day    882 adult female 30day    1001 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    627 embryo 02-04hr    2453 embryo 04-06hr    7992 embryo 06-08hr    11344 embryo 08-10hr    11093 embryo 10-12hr    11664 embryo 12-14hr    10679 embryo 14-16hr    4633 embryo 16-18hr    3857 embryo 18-20hr    4007 embryo 20-22hr    2133 embryo 22-24hr    2863 larva L1    1130 larva L2    1028 larva L3 12hr old    1286 larva L3 puffstage 1-2    1367 larva L3 puffstage 3-6    1856 larva L3 puffstage 7-9    2702 white prepupae new    2493 white prepupae 12hr    4488 white prepupae 24hr    4253 pupae 2d postWPP    3644 pupae 3d postWPP    2026 pupae 4d postWPP    1856 adult male 01day    2652 adult male 05day    2654 adult male 30day    2705 adult female 01day    1353 adult female 05day    882 adult female 30day    1001 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0052486 expression level Developmental Stage   Expression Level embryo 00-02hr    627 embryo 02-04hr    2453 embryo 04-06hr    7992 embryo 06-08hr    11344 embryo 08-10hr    11093 embryo 10-12hr    11664 embryo 12-14hr    10679 embryo 14-16hr    4633 embryo 16-18hr    3857 embryo 18-20hr    4007 embryo 20-22hr    2133 embryo 22-24hr    2863 larva L1    1130 larva L2    1028 larva L3 12hr old    1286 larva L3 puffstage 1-2    1367 larva L3 puffstage 3-6    1856 larva L3 puffstage 7-9    2702 white prepupae new    2493 white prepupae 12hr    4488 white prepupae 24hr    4253 pupae 2d postWPP    3644 pupae 3d postWPP    2026 pupae 4d postWPP    1856 adult male 01day    2652 adult male 05day    2654 adult male 30day    2705 adult female 01day    1353 adult female 05day    882 adult female 30day    1001 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (627) embryo 02-04hr  (2453) embryo 04-06hr  (7992) embryo 06-08hr  (11344) embryo 08-10hr  (11093) embryo 10-12hr  (11664) embryo 12-14hr  (10679) embryo 14-16hr  (4633) embryo 16-18hr  (3857) embryo 18-20hr  (4007) embryo 20-22hr  (2133) embryo 22-24hr  (2863) larva L1  (1130) larva L2  (1028) larva L3 12hr old  (1286) larva L3 puffstage 1-2  (1367) larva L3 puffstage 3-6  (1856) larva L3 puffstage 7-9  (2702) white prepupae new  (2493) white prepupae 12hr  (4488) white prepupae 24hr  (4253) pupae 2d postWPP  (3644) pupae 3d postWPP  (2026) pupae 4d postWPP  (1856) adult male 01day  (2652) adult male 05day  (2654) adult male 30day  (2705) adult female 01day  (1353) adult female 05day  (882) adult female 30day  (1001) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    627 embryo 02-04hr  2453 embryo 04-06hr  (7992) embryo 06-08hr  (11344) embryo 08-10hr  (11093) embryo 10-12hr  (11664) embryo 12-14hr  (10679) embryo 14-16hr  (4633) embryo 16-18hr  (3857) embryo 18-20hr  (4007) embryo 20-22hr  2133 embryo 22-24hr  (2863) larva L1    1130 larva L2    1028 larva L3 12hr old    1286 larva L3 puffstage 1-2    1367 larva L3 puffstage 3-6  1856 larva L3 puffstage 7-9  (2702) white prepupae new  (2493) white prepupae 12hr  (4488) white prepupae 24hr  (4253) pupae 2d postWPP  (3644) pupae 3d postWPP  2026 pupae 4d postWPP  1856 adult male 01day  (2652) adult male 05day  (2654) adult male 30day  (2705) adult female 01day    1353 adult female 05day    882 adult female 30day    1001 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    627 embryo 02-04hr    2453 embryo 04-06hr    7992 embryo 06-08hr  11344 embryo 08-10hr  11093 embryo 10-12hr  11664 embryo 12-14hr  10679 embryo 14-16hr    4633 embryo 16-18hr    3857 embryo 18-20hr    4007 embryo 20-22hr    2133 embryo 22-24hr    2863 larva L1    1130 larva L2    1028 larva L3 12hr old    1286 larva L3 puffstage 1-2    1367 larva L3 puffstage 3-6    1856 larva L3 puffstage 7-9    2702 white prepupae new    2493 white prepupae 12hr    4488 white prepupae 24hr    4253 pupae 2d postWPP    3644 pupae 3d postWPP    2026 pupae 4d postWPP    1856 adult male 01day    2652 adult male 05day    2654 adult male 30day    2705 adult female 01day    1353 adult female 05day    882 adult female 30day    1001 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    627 embryo 02-04hr    2453 embryo 04-06hr    7992 embryo 06-08hr    11344 embryo 08-10hr    11093 embryo 10-12hr    11664 embryo 12-14hr    10679 embryo 14-16hr    4633 embryo 16-18hr    3857 embryo 18-20hr    4007 embryo 20-22hr    2133 embryo 22-24hr    2863 larva L1    1130 larva L2    1028 larva L3 12hr old    1286 larva L3 puffstage 1-2    1367 larva L3 puffstage 3-6    1856 larva L3 puffstage 7-9    2702 white prepupae new    2493 white prepupae 12hr    4488 white prepupae 24hr    4253 pupae 2d postWPP    3644 pupae 3d postWPP    2026 pupae 4d postWPP    1856 adult male 01day    2652 adult male 05day    2654 adult male 30day    2705 adult female 01day    1353 adult female 05day    882 adult female 30day    1001 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0052486    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0052486 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, adult crop, adult Malpighian tubules, adult male accessory gland, adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, larval/adult midgut, larval/adult hindgut, larval Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult testis, larval carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0052486 expression level Tissue   Expression Level Larval Central Nervous System    463.5 Larval Midgut    175.7 Larval Hindgut    310.4 Larval Malpighian Tubules    361.3 Larval Fat Body    134.9 Larval Salivary Gland    204.9 Larval Trachea    156.55 Larval Carcass    205.675 Adult Head    712 Adult Eye    804.55 Adult Brain    1688 Adult Thoracic-Abdominal Ganglion    1853 Adult Crop    521.4 Adult Midgut    341.8 Adult Hindgut    474.7 Adult Malpighian Tubules    557.7 Adult Fat Body    232.1 Adult Salivary Gland    482.7 Adult Heart    297.375 Adult VirginFemale Spermatheca    288.6 Adult InseminatedFemale Spermatheca    225.4 Adult Ovary    145.3 Adult Testis    291.4 Adult Male Accessory Gland    779.2 Adult Carcass    563.8 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    463.5 Larval Midgut    175.7 Larval Hindgut    310.4 Larval Malpighian Tubules    361.3 Larval Fat Body    134.9 Larval Salivary Gland    204.9 Larval Trachea    156.55 Larval Carcass    205.675 Adult Head  (712) Adult Eye  (804.55) Adult Brain  (1688) Adult Thoracic-Abdominal Ganglion  (1853) Adult Crop  521.4 Adult Midgut    341.8 Adult Hindgut    474.7 Adult Malpighian Tubules  (557.7) Adult Fat Body    232.1 Adult Salivary Gland    482.7 Adult Heart    297.375 Adult VirginFemale Spermatheca    288.6 Adult InseminatedFemale Spermatheca    225.4 Adult Ovary    145.3 Adult Testis    291.4 Adult Male Accessory Gland  (779.2) Adult Carcass  (563.8) Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    463.5 Larval Midgut    175.7 Larval Hindgut    310.4 Larval Malpighian Tubules    361.3 Larval Fat Body    134.9 Larval Salivary Gland    204.9 Larval Trachea    156.55 Larval Carcass    205.675 Adult Head    712 Adult Eye    804.55 Adult Brain  (1688) Adult Thoracic-Abdominal Ganglion  (1853) Adult Crop    521.4 Adult Midgut    341.8 Adult Hindgut    474.7 Adult Malpighian Tubules    557.7 Adult Fat Body    232.1 Adult Salivary Gland    482.7 Adult Heart    297.375 Adult VirginFemale Spermatheca    288.6 Adult InseminatedFemale Spermatheca    225.4 Adult Ovary    145.3 Adult Testis    291.4 Adult Male Accessory Gland    779.2 Adult Carcass    563.8 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    463.5 Larval Midgut    175.7 Larval Hindgut    310.4 Larval Malpighian Tubules    361.3 Larval Fat Body    134.9 Larval Salivary Gland    204.9 Larval Trachea    156.55 Larval Carcass    205.675 Adult Head    712 Adult Eye    804.55 Adult Brain    1688 Adult Thoracic-Abdominal Ganglion    1853 Adult Crop    521.4 Adult Midgut    341.8 Adult Hindgut    474.7 Adult Malpighian Tubules    557.7 Adult Fat Body    232.1 Adult Salivary Gland    482.7 Adult Heart    297.375 Adult VirginFemale Spermatheca    288.6 Adult InseminatedFemale Spermatheca    225.4 Adult Ovary    145.3 Adult Testis    291.4 Adult Male Accessory Gland    779.2 Adult Carcass    563.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0052486 expression level Tissue   Expression Level Larval Central Nervous System    463.5 Larval Midgut    175.7 Larval Hindgut    310.4 Larval Malpighian Tubules    361.3 Larval Fat Body    134.9 Larval Salivary Gland    204.9 Larval Trachea    156.55 Larval Carcass    205.675 Adult Head    712 Adult Eye    804.55 Adult Brain    1688 Adult Thoracic-Abdominal Ganglion    1853 Adult Crop    521.4 Adult Midgut    341.8 Adult Hindgut    474.7 Adult Malpighian Tubules    557.7 Adult Fat Body    232.1 Adult Salivary Gland    482.7 Adult Heart    297.375 Adult VirginFemale Spermatheca    288.6 Adult InseminatedFemale Spermatheca    225.4 Adult Ovary    145.3 Adult Testis    291.4 Adult Male Accessory Gland    779.2 Adult Carcass    563.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    463.5 Larval Midgut    175.7 Larval Hindgut    310.4 Larval Malpighian Tubules    361.3 Larval Fat Body    134.9 Larval Salivary Gland    204.9 Larval Trachea    156.55 Larval Carcass    205.675 Adult Head  712 Adult Eye  804.55 Adult Brain  (1688) Adult Thoracic-Abdominal Ganglion  (1853) Adult Crop  521.4 Adult Midgut    341.8 Adult Hindgut    474.7 Adult Malpighian Tubules  557.7 Adult Fat Body    232.1 Adult Salivary Gland    482.7 Adult Heart    297.375 Adult VirginFemale Spermatheca    288.6 Adult InseminatedFemale Spermatheca    225.4 Adult Ovary    145.3 Adult Testis    291.4 Adult Male Accessory Gland  779.2 Adult Carcass  563.8 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    463.5 Larval Midgut    175.7 Larval Hindgut    310.4 Larval Malpighian Tubules    361.3 Larval Fat Body    134.9 Larval Salivary Gland    204.9 Larval Trachea    156.55 Larval Carcass    205.675 Adult Head    712 Adult Eye    804.55 Adult Brain  1688 Adult Thoracic-Abdominal Ganglion  (1853) Adult Crop    521.4 Adult Midgut    341.8 Adult Hindgut    474.7 Adult Malpighian Tubules    557.7 Adult Fat Body    232.1 Adult Salivary Gland    482.7 Adult Heart    297.375 Adult VirginFemale Spermatheca    288.6 Adult InseminatedFemale Spermatheca    225.4 Adult Ovary    145.3 Adult Testis    291.4 Adult Male Accessory Gland    779.2 Adult Carcass    563.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    463.5 Larval Midgut    175.7 Larval Hindgut    310.4 Larval Malpighian Tubules    361.3 Larval Fat Body    134.9 Larval Salivary Gland    204.9 Larval Trachea    156.55 Larval Carcass    205.675 Adult Head    712 Adult Eye    804.55 Adult Brain    1688 Adult Thoracic-Abdominal Ganglion    1853 Adult Crop    521.4 Adult Midgut    341.8 Adult Hindgut    474.7 Adult Malpighian Tubules    557.7 Adult Fat Body    232.1 Adult Salivary Gland    482.7 Adult Heart    297.375 Adult VirginFemale Spermatheca    288.6 Adult InseminatedFemale Spermatheca    225.4 Adult Ovary    145.3 Adult Testis    291.4 Adult Male Accessory Gland    779.2 Adult Carcass    563.8 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    712   NA  Eye    804.55   NA  Brain    1688   463.5  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    1853   NA  Crop    521.4   175.7  Midgut    341.8   310.4  Hindgut    474.7   361.3  Malpighian Tubules    557.7   134.9  Fat Body    232.1   204.9  Salivary Gland    482.7   NA  Heart    297.375   156.55  Trachea    NA   NA  VirginFemale Spermatheca    288.6   NA  InseminatedFemale Spermatheca    225.4   NA  Ovary    145.3   NA  Testis    291.4   NA  Male Accessory Gland    779.2   205.675  Carcass    563.8 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_11
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0052486 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG32486-RD FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for CG32486 Stages(s) 4-6 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal CG32486CB03414 semi-lethal | pupal stage, with Scer\GAL4pnr-MD237 CG32486GD10912 viable CG32486CC00904 Other Phenotypes Allele visible, with Scer\GAL4pnr-MD237 CG32486GD10912 Phenotype manifest in Allele mesothoracic tergum, with Scer\GAL4pnr-MD237 CG32486GD10912 trichogen cell, with Scer\GAL4pnr-MD237 CG32486GD10912
Classical Alleles ( 4 )
For All Classical Alleles Show
Allele of CG32486 Class Mutagen Stocks Known lesion CG32486A077 1 -- CG32486CB03414 P-element activity 0 Yes CG32486CC00904 P-element activity 0 Yes CG32486d00943 P-element activity 0 --
Alleles Carried on Transgenic Constructs ( 2 )
For All Alleles Carried on Transgenic Constructs Show
Allele of CG32486 Class Mutagen Stocks Known lesion CG32486GD10912 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes CG32486GL00391 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD10912} NA P{TRiP.GL00391} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}CG11486EP3109 NA P{XP}CG32486d00943 NA insertion of enhancer trap P{lacW}l(3)SH095SH095 No miscellaneous insertions P{PTT-GB}CG32486CB03414 NA P{PTT-GC}CG32486CC00904 NA PBac{5HPw+}CG32486A077 NA
Gene Ontology: Function, Process & Cellular Component ( 3 unique terms )
Terms Based on Experimental Evidence ( 0 terms )
Terms Based on Predictions or Assertions ( 3 terms )
Molecular Function
CV term Evidence References
ubiquitin-protein ligase activity inferred from electronic annotation with InterPro:IPR013323 (FlyBase Curators et al., 2004-) zinc ion binding inferred from electronic annotation with InterPro:IPR001293, InterPro:IPR001841, InterPro:IPR013323 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
protein ubiquitination inferred from electronic annotation with InterPro:IPR013323 (FlyBase Curators et al., 2004-)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions CG32486 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 63866 DroID - A comprehensive database of gene and protein interactions. • FBgn0052486 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 38367
Orthologs
Genome-wide drosophilid orthologs	Dana\GF10226 Dere\GG14485 Dgri\GH16998 Dmoj\GI11411 Dpse\GA16940 Dsec\GM14088 Dsim\GD13360 Dvir\GJ13607 Dwil\GK12313 Dyak\GE21673
Curated drosophilid orthologs	Dpse\GA16940
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL011575-PA Aedes aegypti (mosquito) XP_396554.2 Apis mellifera (honey bee) XP_001948062.1 Acyrthosiphon pisum (pea aphid) 75398 Branchiostoma floridae (lancelet) ENSBTAP00000037191 Bos taurus (bovine) ENSCAFP00000034779 Canis familiaris (dog) ENSCINP00000022659 Ciona intestinalis (sea squirt) CPIJ018620 Culex pipens (mosquito) ENSCPOP00000015091 Cavia porcellus (guinea pig) ENSCSAVP00000007412 Ciona savignyi (sedentary tunicate) 168572 Capitella spl (polychaete worm) DDB0252617 Dictyostelium discoideum (amoeba) 190307 Daphnia pulex (daphnia) ENSDARP00000081094 Danio rerio (zebra fish) ENSECAP00000015474 Equus caballus (horse) ENSGACP00000007385 Gymnopilus aculeatus (mushroom) 165872 Hypsipyla robusta (cedar shoot borer) ENSP00000292543 Homo sapiens (human) ISCW016475-PA Ixodes scapularis (deer tick) 138168 Lottia gigantea (owl limpet) ENSMODP00000013672 Monodelphis domestica (gray opossum) ENSMMUP00000003581 Macaca mulatta (rhesus monkey) ENSMUSP00000086943 Mus musculus (house mouse) 201051 Nematostella vectensis (starlet anemone) XP_001606747.1 Nasonia vitripennis (wasp) ENSOANP00000023585 Ornithorynchus anatinus (platypus) ENSORLP00000014528 Oryzias latipes (japanese killifish) PHUM000658-PA Pediculus humanus (human body louse) ENSPPYP00000021283 Pongo pygmaeus (orangutan) ENSPTRP00000035418 Pan troglodytes (chimpanzee) ENSRNOP00000042921 Rattus norvegicus (norway rat) XP_784749.1 Strongylocentrotus purpuratus (purple sea urchin) XP_966730.1 Tribolium castaneum (red flour beetle) ENSTNIP00000020100 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000034050 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000021194 Xenopus tropicalis (frog) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV50120 NV13061 LH16740 ISCW016475 FBgn0238922 FBgn0232956 FBgn0214323 FBgn0200829 FBgn0185075 FBgn0169017 JGI_V11_190307 FBgn0076954 FBgn0134172 FBgn0124469 FBgn0106744 FBgn0087267 CPIJ018620 BGIBMGA004143 ACYPI006476 GB19485 AGAP002264 AAEL011575 PB22489 PHUM381100 GLEAN_14541
Stocks & Reagents
Stocks Listed in FlyBase ( 4 )
Bloomington	35465 y1 sc* v1; P{TRiP.GL00391}attP2 16033 y1 w1118; PBac{5HPw+}CG32486A077
VDRC	v41907 w1118; P{GD10912}v41907 v41908 w1118; P{GD10912}v41908
Genomic Clones ( 2 )
	BACR21A02 BACR48E19 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 112 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD47625
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT11507 AT11542 AT17473 AT26465 AT31355 GH16527 HL08007 LD12769 LD38043 RE03279 RE04021 RE04958 RE06457 RE10784 RE14258 RE18610 RE22454 RE22576 RE24549 RE28495 RE30818 RE31609 RE31885 RE36633 RE41281 RE45673 RE49849 RE54348 RE56011 RE58238 RE61983 RE62403 RE63031 RE70768 RH21427 RH27362 RH33558 RH41357 RH50124 RH59122 RH63614 SD15054
Other clones	15852 17807 18703 32066 32727 42196 51772 55502 74786 78168 100530 120396 156404 157056 161832 214275.8 214279.20 214317.16 214385.13 CK01.10C.H7 CK01.12A.H7 CK01.51B.H1 CK01.65C.A6 CK01.79D.C5 CK01.84D.A10 CK01.86A.E1 CK01.105D.E8 CK01.108D.E12 CK01.124D.E1 CK01.142B.B10 EK041929 EK053335 EK053358 EK061911 EK068017 EK088452 EK098052 EK119005 EK119037 EK125716 EK126243 EK129916 EK135408 EK138901 EK143231 EK157025 EK159039 EK165847 EK182146 EK211649 EK223156 EK259541 EK260051 EK280706 EK308009 EP10664 EP13553 EP15430 EP15438 ESG012c.E21_A05 ESG012c.E21_B01 ESG0114d.B21_F09 ESG0119b.B21_D07 FGC022B10 FGM103B07 FGM105F06 FGM222G07 FGM311H05 GH28844
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0052486 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 38367
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: CG14954 CG16745 (Misra, 2001.8.10) Source for merge of: CG32486 anon-WO0118547.267 (FlyBase, 1996-)
Additional comments	Annotations CG14954, CG16745 merged as CG32486 in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003) Source for merge of CG32486 anon-WO0118547.267 was sequence comparison (date:051113). (FlyBase, 1996-)
Other Comments
	Area matching Drosophila EST AA440953. (Davies, 2001.3.15)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_139508 RefSeq (Proteins) • NP_647765 DDBJ / EMBL / GenBank • AA440953 AAF47711 AAK93450 AI544175 AW943926 AX094143 AY052026 CZ471910 CZ471911 Entrez Gene - A searchable database of RefSeq genes. • 38367 UniProtKB/TrEMBL • Q9VZV5
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Zinc finger, TRAF-type (IPR001293) Zinc finger, RING-type (IPR001841) TRAF-like (IPR008974) Zinc finger, RING/FYVE/PHD-type (IPR013083) Seven In Absentia Homolog-type (IPR013323)
Linkouts
	BioGRID - A database of protein and genetic interactions • 63866 DroID - A comprehensive database of gene and protein interactions. • FBgn0052486 DRSC - Results from RNAi screens. • FBgn0052486 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0052486 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG32486-RD FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0052486 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 38367 InParanoid A subset of ortholog calls from InParanoid. • AAEL011575-PA Aedes aegypti (mosquito) XP_396554.2 Apis mellifera (honey bee) XP_001948062.1 Acyrthosiphon pisum (pea aphid) 75398 Branchiostoma floridae (lancelet) ENSBTAP00000037191 Bos taurus (bovine) ENSCAFP00000034779 Canis familiaris (dog) ENSCINP00000022659 Ciona intestinalis (sea squirt) CPIJ018620 Culex pipens (mosquito) ENSCPOP00000015091 Cavia porcellus (guinea pig) ENSCSAVP00000007412 Ciona savignyi (sedentary tunicate) 168572 Capitella spl (polychaete worm) DDB0252617 Dictyostelium discoideum (amoeba) 190307 Daphnia pulex (daphnia) ENSDARP00000081094 Danio rerio (zebra fish) ENSECAP00000015474 Equus caballus (horse) ENSGACP00000007385 Gymnopilus aculeatus (mushroom) 165872 Hypsipyla robusta (cedar shoot borer) ENSP00000292543 Homo sapiens (human) ISCW016475-PA Ixodes scapularis (deer tick) 138168 Lottia gigantea (owl limpet) ENSMODP00000013672 Monodelphis domestica (gray opossum) ENSMMUP00000003581 Macaca mulatta (rhesus monkey) ENSMUSP00000086943 Mus musculus (house mouse) 201051 Nematostella vectensis (starlet anemone) XP_001606747.1 Nasonia vitripennis (wasp) ENSOANP00000023585 Ornithorynchus anatinus (platypus) ENSORLP00000014528 Oryzias latipes (japanese killifish) PHUM000658-PA Pediculus humanus (human body louse) ENSPPYP00000021283 Pongo pygmaeus (orangutan) ENSPTRP00000035418 Pan troglodytes (chimpanzee) ENSRNOP00000042921 Rattus norvegicus (norway rat) XP_784749.1 Strongylocentrotus purpuratus (purple sea urchin) XP_966730.1 Tribolium castaneum (red flour beetle) ENSTNIP00000020100 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000034050 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000021194 Xenopus tropicalis (frog) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 38367 modMine - Data generated by the modENCODE project. • FBgn0052486 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV50120 NV13061 LH16740 ISCW016475 FBgn0238922 FBgn0232956 FBgn0214323 FBgn0200829 FBgn0185075 FBgn0169017 JGI_V11_190307 FBgn0076954 FBgn0134172 FBgn0124469 FBgn0106744 FBgn0087267 CPIJ018620 BGIBMGA004143 ACYPI006476 GB19485 AGAP002264 AAEL011575 PB22489 PHUM381100 GLEAN_14541
Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	anon-WO0118547.267   CG14954   CG16745 (Misra, 2001.8.10, ) CG32486 (Buszczak et al., 2007, Hurlbut et al., 2009, Li et al., 2010, Ni et al., 2011.7.19)
Name Synonym
Secondary FlyBase IDs
	FBgn0035394 FBgn0035395 FBgn0062132
References ( 22 )
Generate a list of	All references relating to this gene ( 22 )
List References by type	Research paper  ( 3 ) Supplementary material  ( 4 ) Personal communication to FlyBase  ( 5 ) FlyBase analysis  ( 4 ) Patent  ( 1 ) DNA/RNA sequence record  ( 1 ) Computer file  ( 3 ) Automatic genome annotation  ( 1 )
Recent research papers ( 1 )
	Li et al., 2010, BMC Cell Biol. 11: 7 Nutritional control of gene expression in Drosophila larvae via TOR, Myc and a novel cis-regulatory element. [FBrf0210137] Show all research papers ...