Gene Dmel\CG32576

FlyGene Wiki Help Close All Open All

General Information

Symbol

Dmel\CG32576

Species

D. melanogaster

Name

Annotation symbol

CG32576

Feature type

protein_coding_gene

FlyBase ID

FBgn0052576

Gene Model Status

Current

Stock availability

1 publicly available

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

14B7-14B8

Sequence location

X:16,179,986..16,182,566 [-]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol Dmel\CG32576 (FBgn0052576). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. An electronic pipeline based on InterPro domains suggests that it is involved in the biological process: vesicle-mediated transport. 3 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Vesicle transport protein, Got1/SFT2-like. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderately high expression. Peak expression observed within 12-24 hour embryonic stages, at stages throughout the larval period, during late pupal stages, in stages of adults of both sexes. Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. Comments on Affy2 ProbeSet: ProbeSet 1628409_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of CG32576. Gene sequence location is X:16179986..16182566.

User Contributed Data

Beta version, feedback welcome

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

Update Feed

Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.

FB2013_03

FB2013_02

All updates

Click here to see a list of all updates to this record from FB2010_08 and on.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

14B7-14B8

Limits computationally determined from genome sequence between P{EP}kat80^EP620&P{EP}caz^EP1564 and P{EP}Cyp1^EP1073&P{EP}Dsp1^EP355

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\CG32576 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.47

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

CG32576-RA

FBtr0074245

NM_167492

1709

128

CG32576-RB

FBtr0100556

NM_001014746

686

140

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

CG32576-PA

FBpp0074024

14.1

128

10.68

NP_727945

AAN09388

CG32576-PB

FBpp0100008

15.3

140

10.57

NP_001014746

AAX52500

Additional Polypeptide Data & Comments

Reported size (kDa)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Vesicle transport protein, Got1/SFT2-like (IPR007305)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		26.35
Larval Midgut		53.5
Larval Hindgut		33.1
Larval Malpighian Tubules		28
Larval Fat Body		23.8
Larval Salivary Gland		45.7
Larval Trachea		19.7
Larval Carcass		51.525
Adult Head		31.5
Adult Eye		38.8
Adult Brain		26
Adult Thoracic-Abdominal Ganglion		43.7
Adult Crop		35.1
Adult Midgut		56.4
Adult Hindgut		43.6
Adult Malpighian Tubules		18.2
Adult Fat Body		39.5
Adult Salivary Gland		87.2
Adult Heart		27.55
Adult VirginFemale Spermatheca		56.8
Adult InseminatedFemale Spermatheca		55.1
Adult Ovary		15.2
Adult Testis		4.9
Adult Male Accessory Gland		41.5
Adult Carcass		54.7
Expression Level Scale		None Low

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		26.35
Larval Midgut		53.5
Larval Hindgut		33.1
Larval Malpighian Tubules		28
Larval Fat Body		23.8
Larval Salivary Gland		45.7
Larval Trachea		19.7
Larval Carcass		51.525
Adult Head		31.5
Adult Eye		38.8
Adult Brain		26
Adult Thoracic-Abdominal Ganglion		43.7
Adult Crop		35.1
Adult Midgut		56.4
Adult Hindgut		43.6
Adult Malpighian Tubules		18.2
Adult Fat Body		39.5
Adult Salivary Gland		87.2
Adult Heart		27.55
Adult VirginFemale Spermatheca		56.8
Adult InseminatedFemale Spermatheca		55.1
Adult Ovary		15.2
Adult Testis		4.9
Adult Male Accessory Gland		41.5
Adult Carcass		54.7
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		26.35
Larval Midgut		53.5
Larval Hindgut		33.1
Larval Malpighian Tubules		28
Larval Fat Body		23.8
Larval Salivary Gland		45.7
Larval Trachea		19.7
Larval Carcass		51.525
Adult Head		31.5
Adult Eye		38.8
Adult Brain		26
Adult Thoracic-Abdominal Ganglion		43.7
Adult Crop		35.1
Adult Midgut		56.4
Adult Hindgut		43.6
Adult Malpighian Tubules		18.2
Adult Fat Body		39.5
Adult Salivary Gland		87.2
Adult Heart		27.55
Adult VirginFemale Spermatheca		56.8
Adult InseminatedFemale Spermatheca		55.1
Adult Ovary		15.2
Adult Testis		4.9
Adult Male Accessory Gland		41.5
Adult Carcass		54.7
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		26.35
Larval Midgut		53.5
Larval Hindgut		33.1
Larval Malpighian Tubules		28
Larval Fat Body		23.8
Larval Salivary Gland		45.7
Larval Trachea		19.7
Larval Carcass		51.525
Adult Head		31.5
Adult Eye		38.8
Adult Brain		26
Adult Thoracic-Abdominal Ganglion		43.7
Adult Crop		35.1
Adult Midgut		56.4
Adult Hindgut		43.6
Adult Malpighian Tubules		18.2
Adult Fat Body		39.5
Adult Salivary Gland		87.2
Adult Heart		27.55
Adult VirginFemale Spermatheca		56.8
Adult InseminatedFemale Spermatheca		55.1
Adult Ovary		15.2
Adult Testis		4.9
Adult Male Accessory Gland		41.5
Adult Carcass		54.7
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		26.35
Larval Midgut		53.5
Larval Hindgut		33.1
Larval Malpighian Tubules		28
Larval Fat Body		23.8
Larval Salivary Gland		45.7
Larval Trachea		19.7
Larval Carcass		51.525
Adult Head		31.5
Adult Eye		38.8
Adult Brain		26
Adult Thoracic-Abdominal Ganglion		43.7
Adult Crop		35.1
Adult Midgut		56.4
Adult Hindgut		43.6
Adult Malpighian Tubules		18.2
Adult Fat Body		39.5
Adult Salivary Gland		87.2
Adult Heart		27.55
Adult VirginFemale Spermatheca		56.8
Adult InseminatedFemale Spermatheca		55.1
Adult Ovary		15.2
Adult Testis		4.9
Adult Male Accessory Gland		41.5
Adult Carcass		54.7
Expression Level Scale		None Low Moderate

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		26.35
Larval Midgut		53.5
Larval Hindgut		33.1
Larval Malpighian Tubules		28
Larval Fat Body		23.8
Larval Salivary Gland		45.7
Larval Trachea		19.7
Larval Carcass		51.525
Adult Head		31.5
Adult Eye		38.8
Adult Brain		26
Adult Thoracic-Abdominal Ganglion		43.7
Adult Crop		35.1
Adult Midgut		56.4
Adult Hindgut		43.6
Adult Malpighian Tubules		18.2
Adult Fat Body		39.5
Adult Salivary Gland		87.2
Adult Heart		27.55
Adult VirginFemale Spermatheca		56.8
Adult InseminatedFemale Spermatheca		55.1
Adult Ovary		15.2
Adult Testis		4.9
Adult Male Accessory Gland		41.5
Adult Carcass		54.7
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		26.35
Larval Midgut		53.5
Larval Hindgut		33.1
Larval Malpighian Tubules		28
Larval Fat Body		23.8
Larval Salivary Gland		45.7
Larval Trachea		19.7
Larval Carcass		51.525
Adult Head		31.5
Adult Eye		38.8
Adult Brain		26
Adult Thoracic-Abdominal Ganglion		43.7
Adult Crop		35.1
Adult Midgut		56.4
Adult Hindgut		43.6
Adult Malpighian Tubules		18.2
Adult Fat Body		39.5
Adult Salivary Gland		87.2
Adult Heart		27.55
Adult VirginFemale Spermatheca		56.8
Adult InseminatedFemale Spermatheca		55.1
Adult Ovary		15.2
Adult Testis		4.9
Adult Male Accessory Gland		41.5
Adult Carcass		54.7
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		26.35
Larval Midgut		53.5
Larval Hindgut		33.1
Larval Malpighian Tubules		28
Larval Fat Body		23.8
Larval Salivary Gland		45.7
Larval Trachea		19.7
Larval Carcass		51.525
Adult Head		31.5
Adult Eye		38.8
Adult Brain		26
Adult Thoracic-Abdominal Ganglion		43.7
Adult Crop		35.1
Adult Midgut		56.4
Adult Hindgut		43.6
Adult Malpighian Tubules		18.2
Adult Fat Body		39.5
Adult Salivary Gland		87.2
Adult Heart		27.55
Adult VirginFemale Spermatheca		56.8
Adult InseminatedFemale Spermatheca		55.1
Adult Ovary		15.2
Adult Testis		4.9
Adult Male Accessory Gland		41.5
Adult Carcass		54.7
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	31.5
NA	Eye	38.8
NA	Brain	26
26.35	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	43.7
NA	Crop	35.1
53.5	Midgut	56.4
33.1	Hindgut	43.6
28	Malpighian Tubules	no informative data
no informative data	Fat Body	39.5
45.7	Salivary Gland	87.2
NA	Heart	27.55
19.7	Trachea	NA
NA	VirginFemale Spermatheca	56.8
NA	InseminatedFemale Spermatheca	55.1
NA	Ovary	no informative data
NA	Testis	4.9
NA	Male Accessory Gland	41.5
51.525	Carcass	54.7

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		2
central nervous system, larvae L3		1
central nervous system, pupae P8		2
head, virgin 1-day female		1
head, virgin 4-day female		1
head, virgin 20-day female		2
head, mated 1-day female		1
head, mated 4-day female		1
head, mated 20-day female		1
head, mated 1-day male		1
head, mated 4-day male		1
head, mated 20-day male		1
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		2
digestive system, 1-day adult		2
digestive system, 4-day adult		1
digestive system, 20-day adult		1
fat body, larvae L3 wandering		1
fat body, white prepupae		1
fat body, pupae P8		4
carcass, larvae L3 wandering		2
carcass, 1-day adult		1
carcass, 4-day adult		2
carcass, 20-day adult		1
ovary, virgin 4-day female		3
ovary, mated 4-day female		1
testis, mated 4-day male		1
accessory gland, mated 4-day male		6
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		2
central nervous system, larvae L3		1
central nervous system, pupae P8		2
head, virgin 1-day female		1
head, virgin 4-day female		1
head, virgin 20-day female		2
head, mated 1-day female		1
head, mated 4-day female		1
head, mated 20-day female		1
head, mated 1-day male		1
head, mated 4-day male		1
head, mated 20-day male		1
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		2
digestive system, 1-day adult		2
digestive system, 4-day adult		1
digestive system, 20-day adult		1
fat body, larvae L3 wandering		1
fat body, white prepupae		1
fat body, pupae P8		4
carcass, larvae L3 wandering		2
carcass, 1-day adult		1
carcass, 4-day adult		2
carcass, 20-day adult		1
ovary, virgin 4-day female		3
ovary, mated 4-day female		1
testis, mated 4-day male		1
accessory gland, mated 4-day male		6
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		2
central nervous system, larvae L3		1
central nervous system, pupae P8		2
head, virgin 1-day female		1
head, virgin 4-day female		1
head, virgin 20-day female		2
head, mated 1-day female		1
head, mated 4-day female		1
head, mated 20-day female		1
head, mated 1-day male		1
head, mated 4-day male		1
head, mated 20-day male		1
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		2
digestive system, 1-day adult		2
digestive system, 4-day adult		1
digestive system, 20-day adult		1
fat body, larvae L3 wandering		1
fat body, white prepupae		1
fat body, pupae P8		4
carcass, larvae L3 wandering		2
carcass, 1-day adult		1
carcass, 4-day adult		2
carcass, 20-day adult		1
ovary, virgin 4-day female		3
ovary, mated 4-day female		1
testis, mated 4-day male		1
accessory gland, mated 4-day male		6
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		2
central nervous system, larvae L3		1
central nervous system, pupae P8		2
head, virgin 1-day female		1
head, virgin 4-day female		1
head, virgin 20-day female		2
head, mated 1-day female		1
head, mated 4-day female		1
head, mated 20-day female		1
head, mated 1-day male		1
head, mated 4-day male		1
head, mated 20-day male		1
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		2
digestive system, 1-day adult		2
digestive system, 4-day adult		1
digestive system, 20-day adult		1
fat body, larvae L3 wandering		1
fat body, white prepupae		1
fat body, pupae P8		4
carcass, larvae L3 wandering		2
carcass, 1-day adult		1
carcass, 4-day adult		2
carcass, 20-day adult		1
ovary, virgin 4-day female		3
ovary, mated 4-day female		1
testis, mated 4-day male		1
accessory gland, mated 4-day male		6
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		2
central nervous system, larvae L3		1
central nervous system, pupae P8		2
head, virgin 1-day female		1
head, virgin 4-day female		1
head, virgin 20-day female		2
head, mated 1-day female		1
head, mated 4-day female		1
head, mated 20-day female		1
head, mated 1-day male		1
head, mated 4-day male		1
head, mated 20-day male		1
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		2
digestive system, 1-day adult		2
digestive system, 4-day adult		1
digestive system, 20-day adult		1
fat body, larvae L3 wandering		1
fat body, white prepupae		1
fat body, pupae P8		4
carcass, larvae L3 wandering		2
carcass, 1-day adult		1
carcass, 4-day adult		2
carcass, 20-day adult		1
ovary, virgin 4-day female		3
ovary, mated 4-day female		1
testis, mated 4-day male		1
accessory gland, mated 4-day male		6
Expression Level Scale		Very low Low Moderate

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		2
central nervous system, larvae L3		1
central nervous system, pupae P8		2
head, virgin 1-day female		1
head, virgin 4-day female		1
head, virgin 20-day female		2
head, mated 1-day female		1
head, mated 4-day female		1
head, mated 20-day female		1
head, mated 1-day male		1
head, mated 4-day male		1
head, mated 20-day male		1
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		2
digestive system, 1-day adult		2
digestive system, 4-day adult		1
digestive system, 20-day adult		1
fat body, larvae L3 wandering		1
fat body, white prepupae		1
fat body, pupae P8		4
carcass, larvae L3 wandering		2
carcass, 1-day adult		1
carcass, 4-day adult		2
carcass, 20-day adult		1
ovary, virgin 4-day female		3
ovary, mated 4-day female		1
testis, mated 4-day male		1
accessory gland, mated 4-day male		6
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		2
central nervous system, larvae L3		1
central nervous system, pupae P8		2
head, virgin 1-day female		1
head, virgin 4-day female		1
head, virgin 20-day female		2
head, mated 1-day female		1
head, mated 4-day female		1
head, mated 20-day female		1
head, mated 1-day male		1
head, mated 4-day male		1
head, mated 20-day male		1
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		2
digestive system, 1-day adult		2
digestive system, 4-day adult		1
digestive system, 20-day adult		1
fat body, larvae L3 wandering		1
fat body, white prepupae		1
fat body, pupae P8		4
carcass, larvae L3 wandering		2
carcass, 1-day adult		1
carcass, 4-day adult		2
carcass, 20-day adult		1
ovary, virgin 4-day female		3
ovary, mated 4-day female		1
testis, mated 4-day male		1
accessory gland, mated 4-day male		6
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		2
central nervous system, larvae L3		1
central nervous system, pupae P8		2
head, virgin 1-day female		1
head, virgin 4-day female		1
head, virgin 20-day female		2
head, mated 1-day female		1
head, mated 4-day female		1
head, mated 20-day female		1
head, mated 1-day male		1
head, mated 4-day male		1
head, mated 20-day male		1
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		2
digestive system, 1-day adult		2
digestive system, 4-day adult		1
digestive system, 20-day adult		1
fat body, larvae L3 wandering		1
fat body, white prepupae		1
fat body, pupae P8		4
carcass, larvae L3 wandering		2
carcass, 1-day adult		1
carcass, 4-day adult		2
carcass, 20-day adult		1
ovary, virgin 4-day female		3
ovary, mated 4-day female		1
testis, mated 4-day male		1
accessory gland, mated 4-day male		6
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderately high expression. Peak expression observed within 12-24 hour embryonic stages, at stages throughout the larval period, during late pupal stages, in stages of adults of both sexes.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		5
embryo 02-04hr		6
embryo 04-06hr		6
embryo 06-08hr		8
embryo 08-10hr		10
embryo 10-12hr		12
embryo 12-14hr		12
embryo 14-16hr		6
embryo 16-18hr		8
embryo 18-20hr		5
embryo 20-22hr		5
embryo 22-24hr		6
larva L1		6
larva L2		13
larva L3 12hr old		8
larva L3 puffstage 1-2		6
larva L3 puffstage 3-6		6
larva L3 puffstage 7-9		8
white prepupae new		9
white prepupae 12hr		6
white prepupae 24hr		5
pupae 2d postWPP		7
pupae 3d postWPP		7
pupae 4d postWPP		5
adult male 01day		8
adult male 05day		8
adult male 30day		5
adult female 01day		5
adult female 05day		9
adult female 30day		7
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		5
embryo 02-04hr		6
embryo 04-06hr		6
embryo 06-08hr		8
embryo 08-10hr		10
embryo 10-12hr		12
embryo 12-14hr		12
embryo 14-16hr		6
embryo 16-18hr		8
embryo 18-20hr		5
embryo 20-22hr		5
embryo 22-24hr		6
larva L1		6
larva L2		13
larva L3 12hr old		8
larva L3 puffstage 1-2		6
larva L3 puffstage 3-6		6
larva L3 puffstage 7-9		8
white prepupae new		9
white prepupae 12hr		6
white prepupae 24hr		5
pupae 2d postWPP		7
pupae 3d postWPP		7
pupae 4d postWPP		5
adult male 01day		8
adult male 05day		8
adult male 30day		5
adult female 01day		5
adult female 05day		9
adult female 30day		7
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		5
embryo 02-04hr		6
embryo 04-06hr		6
embryo 06-08hr		8
embryo 08-10hr		10
embryo 10-12hr		12
embryo 12-14hr		12
embryo 14-16hr		6
embryo 16-18hr		8
embryo 18-20hr		5
embryo 20-22hr		5
embryo 22-24hr		6
larva L1		6
larva L2		13
larva L3 12hr old		8
larva L3 puffstage 1-2		6
larva L3 puffstage 3-6		6
larva L3 puffstage 7-9		8
white prepupae new		9
white prepupae 12hr		6
white prepupae 24hr		5
pupae 2d postWPP		7
pupae 3d postWPP		7
pupae 4d postWPP		5
adult male 01day		8
adult male 05day		8
adult male 30day		5
adult female 01day		5
adult female 05day		9
adult female 30day		7
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		5
embryo 02-04hr		6
embryo 04-06hr		6
embryo 06-08hr		8
embryo 08-10hr		10
embryo 10-12hr		12
embryo 12-14hr		12
embryo 14-16hr		6
embryo 16-18hr		8
embryo 18-20hr		5
embryo 20-22hr		5
embryo 22-24hr		6
larva L1		6
larva L2		13
larva L3 12hr old		8
larva L3 puffstage 1-2		6
larva L3 puffstage 3-6		6
larva L3 puffstage 7-9		8
white prepupae new		9
white prepupae 12hr		6
white prepupae 24hr		5
pupae 2d postWPP		7
pupae 3d postWPP		7
pupae 4d postWPP		5
adult male 01day		8
adult male 05day		8
adult male 30day		5
adult female 01day		5
adult female 05day		9
adult female 30day		7
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		5
embryo 02-04hr		6
embryo 04-06hr		6
embryo 06-08hr		8
embryo 08-10hr		10
embryo 10-12hr		12
embryo 12-14hr		12
embryo 14-16hr		6
embryo 16-18hr		8
embryo 18-20hr		5
embryo 20-22hr		5
embryo 22-24hr		6
larva L1		6
larva L2		13
larva L3 12hr old		8
larva L3 puffstage 1-2		6
larva L3 puffstage 3-6		6
larva L3 puffstage 7-9		8
white prepupae new		9
white prepupae 12hr		6
white prepupae 24hr		5
pupae 2d postWPP		7
pupae 3d postWPP		7
pupae 4d postWPP		5
adult male 01day		8
adult male 05day		8
adult male 30day		5
adult female 01day		5
adult female 05day		9
adult female 30day		7
Expression Level Scale		Very low Low Moderate

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		5
embryo 02-04hr		6
embryo 04-06hr		6
embryo 06-08hr		8
embryo 08-10hr		10
embryo 10-12hr		12
embryo 12-14hr		12
embryo 14-16hr		6
embryo 16-18hr		8
embryo 18-20hr		5
embryo 20-22hr		5
embryo 22-24hr		6
larva L1		6
larva L2		13
larva L3 12hr old		8
larva L3 puffstage 1-2		6
larva L3 puffstage 3-6		6
larva L3 puffstage 7-9		8
white prepupae new		9
white prepupae 12hr		6
white prepupae 24hr		5
pupae 2d postWPP		7
pupae 3d postWPP		7
pupae 4d postWPP		5
adult male 01day		8
adult male 05day		8
adult male 30day		5
adult female 01day		5
adult female 05day		9
adult female 30day		7
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		5
embryo 02-04hr		6
embryo 04-06hr		6
embryo 06-08hr		8
embryo 08-10hr		10
embryo 10-12hr		12
embryo 12-14hr		12
embryo 14-16hr		6
embryo 16-18hr		8
embryo 18-20hr		5
embryo 20-22hr		5
embryo 22-24hr		6
larva L1		6
larva L2		13
larva L3 12hr old		8
larva L3 puffstage 1-2		6
larva L3 puffstage 3-6		6
larva L3 puffstage 7-9		8
white prepupae new		9
white prepupae 12hr		6
white prepupae 24hr		5
pupae 2d postWPP		7
pupae 3d postWPP		7
pupae 4d postWPP		5
adult male 01day		8
adult male 05day		8
adult male 30day		5
adult female 01day		5
adult female 05day		9
adult female 30day		7
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		5
embryo 02-04hr		6
embryo 04-06hr		6
embryo 06-08hr		8
embryo 08-10hr		10
embryo 10-12hr		12
embryo 12-14hr		12
embryo 14-16hr		6
embryo 16-18hr		8
embryo 18-20hr		5
embryo 20-22hr		5
embryo 22-24hr		6
larva L1		6
larva L2		13
larva L3 12hr old		8
larva L3 puffstage 1-2		6
larva L3 puffstage 3-6		6
larva L3 puffstage 7-9		8
white prepupae new		9
white prepupae 12hr		6
white prepupae 24hr		5
pupae 2d postWPP		7
pupae 3d postWPP		7
pupae 4d postWPP		5
adult male 01day		8
adult male 05day		8
adult male 30day		5
adult female 01day		5
adult female 05day		9
adult female 30day		7
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		6
Schneider line 2 Sg4		7
embryonic 1182-4H		2
embryonic GM2		6
embryonic Kc167		12
embryonic S1		2
embryonic S3		11
leg disc CME L1		2
wing disc CME-W2		7
wing disc ML-DmD8		4
wing disc ML-DmD9		1
wing disc ML-DmD16-c3		3
wing disc ML-DmD21		1
wing disc ML-DmD32		2
haltere disc ML-DmD17-c3		3
eye-antennal disc ML-DmD11		3
antennal disc ML-DmD20-c5		5
mixed discs ML-DmD4-c1		3
CNS ML-DmBG1-c1		5
CNS ML-DmBG2-c2		6
tumorous blood cells mbn2		9
ovary fGS/OSS		1
ovary OSC		0
ovary OSS		1
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		6
Schneider line 2 Sg4		7
embryonic 1182-4H		2
embryonic GM2		6
embryonic Kc167		12
embryonic S1		2
embryonic S3		11
leg disc CME L1		2
wing disc CME-W2		7
wing disc ML-DmD8		4
wing disc ML-DmD9		1
wing disc ML-DmD16-c3		3
wing disc ML-DmD21		1
wing disc ML-DmD32		2
haltere disc ML-DmD17-c3		3
eye-antennal disc ML-DmD11		3
antennal disc ML-DmD20-c5		5
mixed discs ML-DmD4-c1		3
CNS ML-DmBG1-c1		5
CNS ML-DmBG2-c2		6
tumorous blood cells mbn2		9
ovary fGS/OSS		1
ovary OSC		0
ovary OSS		1
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		6
Schneider line 2 Sg4		7
embryonic 1182-4H		2
embryonic GM2		6
embryonic Kc167		12
embryonic S1		2
embryonic S3		11
leg disc CME L1		2
wing disc CME-W2		7
wing disc ML-DmD8		4
wing disc ML-DmD9		1
wing disc ML-DmD16-c3		3
wing disc ML-DmD21		1
wing disc ML-DmD32		2
haltere disc ML-DmD17-c3		3
eye-antennal disc ML-DmD11		3
antennal disc ML-DmD20-c5		5
mixed discs ML-DmD4-c1		3
CNS ML-DmBG1-c1		5
CNS ML-DmBG2-c2		6
tumorous blood cells mbn2		9
ovary fGS/OSS		1
ovary OSC		0
ovary OSS		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		6
Schneider line 2 Sg4		7
embryonic 1182-4H		2
embryonic GM2		6
embryonic Kc167		12
embryonic S1		2
embryonic S3		11
leg disc CME L1		2
wing disc CME-W2		7
wing disc ML-DmD8		4
wing disc ML-DmD9		1
wing disc ML-DmD16-c3		3
wing disc ML-DmD21		1
wing disc ML-DmD32		2
haltere disc ML-DmD17-c3		3
eye-antennal disc ML-DmD11		3
antennal disc ML-DmD20-c5		5
mixed discs ML-DmD4-c1		3
CNS ML-DmBG1-c1		5
CNS ML-DmBG2-c2		6
tumorous blood cells mbn2		9
ovary fGS/OSS		1
ovary OSC		0
ovary OSS		1
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		6
Schneider line 2 Sg4		7
embryonic 1182-4H		2
embryonic GM2		6
embryonic Kc167		12
embryonic S1		2
embryonic S3		11
leg disc CME L1		2
wing disc CME-W2		7
wing disc ML-DmD8		4
wing disc ML-DmD9		1
wing disc ML-DmD16-c3		3
wing disc ML-DmD21		1
wing disc ML-DmD32		2
haltere disc ML-DmD17-c3		3
eye-antennal disc ML-DmD11		3
antennal disc ML-DmD20-c5		5
mixed discs ML-DmD4-c1		3
CNS ML-DmBG1-c1		5
CNS ML-DmBG2-c2		6
tumorous blood cells mbn2		9
ovary fGS/OSS		1
ovary OSC		0
ovary OSS		1
Expression Level Scale		Very low Low Moderate

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		6
Schneider line 2 Sg4		7
embryonic 1182-4H		2
embryonic GM2		6
embryonic Kc167		12
embryonic S1		2
embryonic S3		11
leg disc CME L1		2
wing disc CME-W2		7
wing disc ML-DmD8		4
wing disc ML-DmD9		1
wing disc ML-DmD16-c3		3
wing disc ML-DmD21		1
wing disc ML-DmD32		2
haltere disc ML-DmD17-c3		3
eye-antennal disc ML-DmD11		3
antennal disc ML-DmD20-c5		5
mixed discs ML-DmD4-c1		3
CNS ML-DmBG1-c1		5
CNS ML-DmBG2-c2		6
tumorous blood cells mbn2		9
ovary fGS/OSS		1
ovary OSC		0
ovary OSS		1
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		6
Schneider line 2 Sg4		7
embryonic 1182-4H		2
embryonic GM2		6
embryonic Kc167		12
embryonic S1		2
embryonic S3		11
leg disc CME L1		2
wing disc CME-W2		7
wing disc ML-DmD8		4
wing disc ML-DmD9		1
wing disc ML-DmD16-c3		3
wing disc ML-DmD21		1
wing disc ML-DmD32		2
haltere disc ML-DmD17-c3		3
eye-antennal disc ML-DmD11		3
antennal disc ML-DmD20-c5		5
mixed discs ML-DmD4-c1		3
CNS ML-DmBG1-c1		5
CNS ML-DmBG2-c2		6
tumorous blood cells mbn2		9
ovary fGS/OSS		1
ovary OSC		0
ovary OSS		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		6
Schneider line 2 Sg4		7
embryonic 1182-4H		2
embryonic GM2		6
embryonic Kc167		12
embryonic S1		2
embryonic S3		11
leg disc CME L1		2
wing disc CME-W2		7
wing disc ML-DmD8		4
wing disc ML-DmD9		1
wing disc ML-DmD16-c3		3
wing disc ML-DmD21		1
wing disc ML-DmD32		2
haltere disc ML-DmD17-c3		3
eye-antennal disc ML-DmD11		3
antennal disc ML-DmD20-c5		5
mixed discs ML-DmD4-c1		3
CNS ML-DmBG1-c1		5
CNS ML-DmBG2-c2		6
tumorous blood cells mbn2		9
ovary fGS/OSS		1
ovary OSC		0
ovary OSS		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		0
cold shock, 4-day adult		0
heat shock, 4-day adult		1
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		4
Cadmium 50 mM 48 hrs, 4-day adult		2
Cadmium 100 mM 48 hrs, 4-day adult		2
Copper 0.5 mM 12 hrs, larvae L3		1
Copper 15 mM 48 hrs, 4-day adult		1
Zinc 5 mM 12 hrs, larvae L3		0
Zinc 4.5 mM 48 hrs, 4-day adult		2
Ethanol 2.5% 3 hrs, larvae L3		1
Ethanol 5% 3 hrs, larvae L3		3
Ethanol 10% 3 hrs, larvae L3		1
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		2
Caffeine 25 mg/ml 48 hrs, 4-day adult		0
Paraquat 5 mM 48 hrs, 4-day adult		2
Paraquat 10 mM 48 hrs, 4-day adult		2
Rotenone 2 μg 12 hrs, larvae L3		3
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		0
cold shock, 4-day adult		0
heat shock, 4-day adult		1
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		4
Cadmium 50 mM 48 hrs, 4-day adult		2
Cadmium 100 mM 48 hrs, 4-day adult		2
Copper 0.5 mM 12 hrs, larvae L3		1
Copper 15 mM 48 hrs, 4-day adult		1
Zinc 5 mM 12 hrs, larvae L3		0
Zinc 4.5 mM 48 hrs, 4-day adult		2
Ethanol 2.5% 3 hrs, larvae L3		1
Ethanol 5% 3 hrs, larvae L3		3
Ethanol 10% 3 hrs, larvae L3		1
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		2
Caffeine 25 mg/ml 48 hrs, 4-day adult		0
Paraquat 5 mM 48 hrs, 4-day adult		2
Paraquat 10 mM 48 hrs, 4-day adult		2
Rotenone 2 μg 12 hrs, larvae L3		3
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		0
cold shock, 4-day adult		0
heat shock, 4-day adult		1
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		4
Cadmium 50 mM 48 hrs, 4-day adult		2
Cadmium 100 mM 48 hrs, 4-day adult		2
Copper 0.5 mM 12 hrs, larvae L3		1
Copper 15 mM 48 hrs, 4-day adult		1
Zinc 5 mM 12 hrs, larvae L3		0
Zinc 4.5 mM 48 hrs, 4-day adult		2
Ethanol 2.5% 3 hrs, larvae L3		1
Ethanol 5% 3 hrs, larvae L3		3
Ethanol 10% 3 hrs, larvae L3		1
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		2
Caffeine 25 mg/ml 48 hrs, 4-day adult		0
Paraquat 5 mM 48 hrs, 4-day adult		2
Paraquat 10 mM 48 hrs, 4-day adult		2
Rotenone 2 μg 12 hrs, larvae L3		3
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		0
cold shock, 4-day adult		0
heat shock, 4-day adult		1
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		4
Cadmium 50 mM 48 hrs, 4-day adult		2
Cadmium 100 mM 48 hrs, 4-day adult		2
Copper 0.5 mM 12 hrs, larvae L3		1
Copper 15 mM 48 hrs, 4-day adult		1
Zinc 5 mM 12 hrs, larvae L3		0
Zinc 4.5 mM 48 hrs, 4-day adult		2
Ethanol 2.5% 3 hrs, larvae L3		1
Ethanol 5% 3 hrs, larvae L3		3
Ethanol 10% 3 hrs, larvae L3		1
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		2
Caffeine 25 mg/ml 48 hrs, 4-day adult		0
Paraquat 5 mM 48 hrs, 4-day adult		2
Paraquat 10 mM 48 hrs, 4-day adult		2
Rotenone 2 μg 12 hrs, larvae L3		3
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		0
cold shock, 4-day adult		0
heat shock, 4-day adult		1
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		4
Cadmium 50 mM 48 hrs, 4-day adult		2
Cadmium 100 mM 48 hrs, 4-day adult		2
Copper 0.5 mM 12 hrs, larvae L3		1
Copper 15 mM 48 hrs, 4-day adult		1
Zinc 5 mM 12 hrs, larvae L3		0
Zinc 4.5 mM 48 hrs, 4-day adult		2
Ethanol 2.5% 3 hrs, larvae L3		1
Ethanol 5% 3 hrs, larvae L3		3
Ethanol 10% 3 hrs, larvae L3		1
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		2
Caffeine 25 mg/ml 48 hrs, 4-day adult		0
Paraquat 5 mM 48 hrs, 4-day adult		2
Paraquat 10 mM 48 hrs, 4-day adult		2
Rotenone 2 μg 12 hrs, larvae L3		3
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		0
cold shock, 4-day adult		0
heat shock, 4-day adult		1
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		4
Cadmium 50 mM 48 hrs, 4-day adult		2
Cadmium 100 mM 48 hrs, 4-day adult		2
Copper 0.5 mM 12 hrs, larvae L3		1
Copper 15 mM 48 hrs, 4-day adult		1
Zinc 5 mM 12 hrs, larvae L3		0
Zinc 4.5 mM 48 hrs, 4-day adult		2
Ethanol 2.5% 3 hrs, larvae L3		1
Ethanol 5% 3 hrs, larvae L3		3
Ethanol 10% 3 hrs, larvae L3		1
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		2
Caffeine 25 mg/ml 48 hrs, 4-day adult		0
Paraquat 5 mM 48 hrs, 4-day adult		2
Paraquat 10 mM 48 hrs, 4-day adult		2
Rotenone 2 μg 12 hrs, larvae L3		3
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		0
cold shock, 4-day adult		0
heat shock, 4-day adult		1
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		4
Cadmium 50 mM 48 hrs, 4-day adult		2
Cadmium 100 mM 48 hrs, 4-day adult		2
Copper 0.5 mM 12 hrs, larvae L3		1
Copper 15 mM 48 hrs, 4-day adult		1
Zinc 5 mM 12 hrs, larvae L3		0
Zinc 4.5 mM 48 hrs, 4-day adult		2
Ethanol 2.5% 3 hrs, larvae L3		1
Ethanol 5% 3 hrs, larvae L3		3
Ethanol 10% 3 hrs, larvae L3		1
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		2
Caffeine 25 mg/ml 48 hrs, 4-day adult		0
Paraquat 5 mM 48 hrs, 4-day adult		2
Paraquat 10 mM 48 hrs, 4-day adult		2
Rotenone 2 μg 12 hrs, larvae L3		3
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		0
cold shock, 4-day adult		0
heat shock, 4-day adult		1
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		4
Cadmium 50 mM 48 hrs, 4-day adult		2
Cadmium 100 mM 48 hrs, 4-day adult		2
Copper 0.5 mM 12 hrs, larvae L3		1
Copper 15 mM 48 hrs, 4-day adult		1
Zinc 5 mM 12 hrs, larvae L3		0
Zinc 4.5 mM 48 hrs, 4-day adult		2
Ethanol 2.5% 3 hrs, larvae L3		1
Ethanol 5% 3 hrs, larvae L3		3
Ethanol 10% 3 hrs, larvae L3		1
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		2
Caffeine 25 mg/ml 48 hrs, 4-day adult		0
Paraquat 5 mM 48 hrs, 4-day adult		2
Paraquat 10 mM 48 hrs, 4-day adult		2
Rotenone 2 μg 12 hrs, larvae L3		3
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010, Graveley et al., 2011)

External Data & Images

Linkouts

BDGP expression data

•

RE29988

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0052576

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG32576-RA

Alleles & Phenotypes

Summary of Allele Phenotypes

Phenotype manifest in

Allele

Classical Alleles ( 0 )

For All Classical Alleles Show

Allele of CG32576	Class	Mutagen	Stocks	Known lesion

Alleles Carried on Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Allele of CG32576	Mutagen	Stocks	Known lesion
CG32576^KK108973	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
CG32576^KK115522	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes
CG32576^Scer\UAS.x10	in vitro construct - regulatory fusion	0	Yes

Aneuploid Aberrations

Disrupted in

Df(1)383

(Wang et al., 2011, Wang et al., 2011)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

P{Mae-UAS.6.11}B0176^B0176

Gene Ontology: Function, Process & Cellular Component ( 1 unique term )

There is no information supporting a cellular component or molecular function term for this gene.

(Based on a review of available data before: 2006)

Terms Based on Experimental Evidence ( 0 terms )

Terms Based on Predictions or Assertions ( 1 term )

Molecular Function ( 0 terms)

Biological Process

CV term

Evidence

References

vesicle-mediated transport

inferred from electronic annotation with InterPro:IPR007305

(FlyBase Curators et al., 2004-)

Cellular Component ( 0 terms)

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

CG32576 allele

Gene

References

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0052576

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

318095

Orthologs

OrthoDB Orthologs (40) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG6RR742

Dipteran inclusive ortholog search

EOG64BBBR

Insect inclusive ortholog search

EOG69W1C5

Arthropod inclusive ortholog search

EOG6SJ3XK

Metazoa inclusive ortholog search

EOG6QK4HR

Orthologs in Drosophila Species (EOG6RR742)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila persimilis

Dper\GL21326

Drosophila willistoni

Dwil\GK25203

Drosophila virilis

Dvir\GJ18545

Drosophila mojavensis

Dmoj\GI10899

Drosophila grimshawi

Dgri\GH12153

Orthologs in non-Drosophila Dipterans (EOG64BBBR)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL010895

Anopheles gambiae

Malaria mosquito

Agam\AGAP002357

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ005942

Orthologs in non-Dipteran Insects (EOG69W1C5)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis mellifera

Western honey bee

Amel\LOC412806

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG007300

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH17193

Atta cephalotes

Leafcutter ant

Acep\ACEP27759

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO23123

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL18077

Linepithema humile

Argentine ant

Lhum\LH21306

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB19963

Solenopsis invicta

Red fire ant

Sinv\SINV14819

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI002493

Bombyx mori

Silkmoth

Bmor\BGIBMGA009559

Pediculus humanus

Human body louse

Phum\PHUM580820

Orthologs in non-Insect Arthropods (EOG6SJ3XK)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Ixodes scapularis

Deer tick

Isca\ISCW021755

Orthologs in non-Arthropod Metazoa (EOG6QK4HR)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Caenorhabditis elegans

Nematode

Cele\F41C3.4

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_023058gn

Danio rerio

Zebrafish

Drer\ENSDARG00000088933

Danio rerio

Zebrafish

Drer\golt1b

Danio rerio

Zebrafish

Drer\si:dkey-13i19.9

Xenopus tropicalis

Western clawed frog

Xtro\golt1b

Gallus gallus

Domestic chicken

Ggal\GOLT1B

Mus musculus

House mouse

Mmus\Golt1b

Mus musculus

House mouse

Mmus\Golt1a

Rattus norvegicus

Norway rat

Rnor\Kiss1

Rattus norvegicus

Norway rat

Rnor\Golt1b

Homo sapiens

Human

Hsap\GOLT1B

Homo sapiens

Human

Hsap\GOLT1A

Human Orthologs (2)

Gene

OMIM

HGNC

Hsap\GOLT1B

Hsap\GOLT1A

AAA Orthologs (10) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila persimilis

Dper\GL21326

Drosophila willistoni

Dwil\GK25203

Drosophila virilis

Dvir\GJ18545

Drosophila mojavensis

Dmoj\GI10899

Drosophila grimshawi

Dgri\GH12153

Stocks & Reagents

Stocks Listed in FlyBase ( 1 )

VDRC

v101529

P{KK108973}VIE-260B

Genomic Clones ( 2 )

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 15 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

RE29988

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Linkouts

DRSC - Results from RNAi screens.

•

FBgn0052576

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

318095

Antibody Information

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for database merge of

Source for merge of: CG32576 anon-AE003501.1

(FlyBase, 1996-)

Source for merge of: CG32576 BcDNA:RE29988

(FlyBase, 1996-)

Source for merge of: CG32576 NEST:bs01h05

(FlyBase, 1996-)

Additional comments

Source for merge of CG32576 NEST:bs01h05 was sequence comparison (date:030903).

(FlyBase, 1996-)

Source for merge of CG32576 BcDNA:RE29988 was a shared cDNA (date:030728).

(FlyBase, 1996-)

Source for merge of CG32576 anon-AE003501.1 was sequence comparison (date:030626).

(FlyBase, 1996-)

Other Comments

New annotation (CG32576) in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

RefSeq (Proteins)

•

DDBJ / EMBL / GenBank

•

Entrez Gene - A searchable database of RefSeq genes.

•

318095

UniProtKB/TrEMBL

•

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Vesicle transport protein, Got1/SFT2-like (IPR007305)

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

•

RE29988

Linkouts

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0052576

DRSC - Results from RNAi screens.

•

FBgn0052576

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0052576

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG32576-RA

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0052576

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

318095

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

318095

modMine - Data generated by the modENCODE project.

•

FBgn0052576

Synonyms & Secondary IDs ( 5 )

Reported As

Symbol Synonym

anon-AE003501.1

BcDNA:RE29988

CG32576

(Legube et al., 2006, Grieder et al., 2007, Wang et al., 2011, Keleman et al., 2009.8.5, Xia et al., 2012)

NEST:bs01h05

unnamed

(Robertson, 2001.1.8)

Name Synonym

Secondary FlyBase IDs

FBgn0043544
FBgn0045434
FBgn0047127

References ( 15 )

Research paper

Xia et al., 2012, Mol. Neurodegener. 7: 10: Motor neuron apoptosis and neuromuscular junction perturbation are prominent features in a Drosophila model of Fus-mediated ALS. [FBrf0218020]
Wang et al., 2011, J. Clin. Invest. 121(10): 4118--4126: The ALS-associated proteins FUS and TDP-43 function together to affect Drosophila locomotion and life span. [FBrf0216240]
Legube et al., 2006, Genes Dev. 20(7): 871--883: X-chromosome-wide profiling of MSL-1 distribution and dosage compensation in Drosophila. [FBrf0190549]

Supplementary material

Graveley et al., 2011, Nature 471(7339):: Supplementary Table 33. Fuzzy Cluster Members. [FBrf0213980]
The modENCODE Consortium, 2010, Science 330(6012):: DataS14: Gene co-expression clusters. [FBrf0213979]
Grieder et al., 2007, Genetics 175(4):: Supplemental Data. [FBrf0204785]

Personal communication to FlyBase

Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510]
FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms.: Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215]
Oliver, 2001.8.16, Genome Res.:: File from B. Oliver containing information on ESTs expressed in the Drosophila testis, described in Andrews et al., 2000, Genome Res. 10(12): 2030-2043 (FBrf0132348). [FBrf0137492]
Robertson, 2001.1.8, FlyBase error report on Mo n Jan 8 14:31:14 2001.: FlyBase error report on Mo n Jan 8 14:31:14 2001. [FBrf0133242]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file): Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078]

FlyBase analysis

FlyBase Genome Annotators, 2002-2003, Changes affecting gene model number or type in releases 3.0 and 3.1 of the annotated D.melanogaster genome.: Changes affecting gene model number or type in releases 3.0 and 3.1 of the annotated D.melanogaster genome. [FBrf0148886]
FlyBase Curators, 2002-, Use of the ND evidence code for Gene Ontology (GO) terms in FlyBase.: Use of the ND evidence code for Gene Ontology (GO) terms in FlyBase. [FBrf0159398]
FlyBase, 1996-, FlyBase inference based on genome sequence analysis.: FlyBase inference based on genome sequence analysis. [FBrf0104946]
FlyBase, 1992-, FlyBase curation.: FlyBase curation. [FBrf0105495]