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General Information
Symbol
Dmel\Atg8a
Species
D. melanogaster
Name
Autophagy-related 8a
Annotation Symbol
CG32672
Feature Type
FlyBase ID
FBgn0052672
Gene Model Status
Stock Availability
Gene Snapshot
Autophagy-related 8a (Atg8a) encodes a ubiquitin-like protein that is conjugated to phosphatidylethanolamine, and this lipid modification anchors it into the membrane of forming and completed autophagosomes. Atg8a product has roles in autophagosome formation, maintaining neuromuscular function and normal lifespan. [Date last reviewed: 2019-03-07]
Also Known As

Atg8, LC3, Autophagy-specific gene 8a, DrAtg8a

Key Links
Genomic Location
Cytogenetic map
Sequence location
X:10,764,878..10,768,338 [-]
Recombination map

1-32

RefSeq locus
NC_004354 REGION:10764878..10768338
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (21 terms)
Molecular Function (5 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
inferred from physical interaction with FLYBASE:Atg1; FB:FBgn0260945
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000861087
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000861056
(assigned by GO_Central )
inferred from sequence or structural similarity with SGD:S000000174
inferred from biological aspect of ancestor with PANTHER:PTN000103525
(assigned by GO_Central )
Biological Process (10 terms)
Terms Based on Experimental Evidence (6 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:daw; FB:FBgn0031461
inferred from expression pattern
inferred from mutant phenotype
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000103525
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000861056
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000103525
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000103522
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000103523
(assigned by GO_Central )
Cellular Component (6 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000103523
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000103525
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000103523
(assigned by GO_Central )
Gene Group (FlyBase)
Protein Family (UniProt)
-
Summaries
Gene Group (FlyBase)
AUTOPHAGY-RELATED GENES -
The Autophagy-related genes (Atg), characterized extensively in yeast, are a set of approximately twenty evolutionarily conserved genes required for autophagy. Autophagy (specifically, macroautophagy) is the process of bulk degradation of cytoplasmic material. It takes place by the assembly of a double membrane vesicle, the autophagosome, which delivers cargo to the lysosome for recycling. (Adapted from FBrf0219844 and FBrf020902).
Gene Model and Products
Number of Transcripts
3
Number of Unique Polypeptides
3

Please see the GBrowse view of Dmel\Atg8a or the JBrowse view of Dmel\Atg8a for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.55

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.47

Gene model reviewed during 5.56

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0071498
1214
121
FBtr0346375
1010
96
FBtr0346376
1183
107
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0071427
14.4
121
9.44
FBpp0312069
11.3
96
7.75
FBpp0312070
12.6
107
8.45
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Atg8a using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
RT-PCR
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

qRT-PCR of adult female heads assayed at weekly intervals shows that transcript levels decline significantly by 3 weeks and remain supressed.

Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Atg8a in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 11 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 31 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Atg8a
Transgenic constructs containing regulatory region of Atg8a
Deletions and Duplications ( 1 )
Partially disrupted in
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (9)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
12 of 15
Yes
Yes
1  
8 of 15
No
Yes
5 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
2 of 15
No
Yes
2 of 15
No
Yes
2 of 15
No
Yes
1  
1 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (5)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
13 of 15
Yes
Yes
8 of 15
No
Yes
5 of 15
No
Yes
2 of 15
No
Yes
1 of 15
No
Yes
Rattus norvegicus (Norway rat) (6)
11 of 13
Yes
Yes
5 of 13
No
Yes
2 of 13
No
Yes
2 of 13
No
Yes
2 of 13
No
Yes
1 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (7)
9 of 12
Yes
Yes
3 of 12
No
Yes
2 of 12
No
Yes
2 of 12
No
Yes
2 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
Danio rerio (Zebrafish) (8)
14 of 15
Yes
Yes
12 of 15
No
Yes
6 of 15
No
Yes
5 of 15
No
Yes
2 of 15
No
Yes
2 of 15
No
Yes
2 of 15
No
Yes
2 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (3)
13 of 15
Yes
Yes
3 of 15
No
Yes
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (9)
9 of 9
Yes
Yes
8 of 9
No
Yes
8 of 9
No
Yes
8 of 9
No
Yes
8 of 9
No
Yes
8 of 9
No
Yes
7 of 9
No
Yes
3 of 9
No
Yes
3 of 9
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (1)
13 of 15
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
11 of 12
Yes
Yes
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190IKM )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150F7J )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0IZU )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0J49 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G10QX )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (1)
8 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Allele
Disease
Evidence
References
Potential Models Based on Orthology ( 0 )
Human Ortholog
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 4 )
Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Interactions Browser

Please see the Physical Interaction reports below for full details
protein-protein
Physical Interaction
Assay
References
RNA-protein
Physical Interaction
Assay
References
Summary of Genetic Interactions
esyN Network Diagram
esyN Network Key:
Suppression
Enhancement

Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
External Data
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
MIST (genetic) - An integrated Molecular Interaction Database
MIST (protein-protein) - An integrated Molecular Interaction Database
Pathways
Signaling Pathways (FlyBase)
Metabolic Pathways
External Data
Linkouts
KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
Genomic Location and Detailed Mapping Data
Chromosome (arm)
X
Recombination map

1-32

Cytogenetic map
Sequence location
X:10,764,878..10,768,338 [-]
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
9E6-9E7
Limits computationally determined from genome sequence between P{EP}EP1645 and P{EP}Atg8aEP362&P{EP}CG1826EP1098
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (20)
Genomic Clones (9)
 

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (243)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced
BDGP DGC clones
Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    Other clones
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of

    Source for identity of: Atg8a CG32672

    Source for database merge of

    Source for merge of: CG32672 BcDNA:LD05816

    Additional comments

    The Atg8b gene may have been derived from the Atg8a gene by retroposition.

    Source for merge of CG32672 BcDNA:LD05816 was a shared cDNA ( date:030728 ).

    "l(1)G0076" may affect "CG32672".

    Other Comments
    Origin and Etymology
    Discoverer
    Etymology
    Identification
    External Crossreferences and Linkouts ( 50 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    InterPro - A database of protein families, domains and functional sites
    KEGG Genes - Molecular building blocks of life in the genomic space.
    modMine - A data warehouse for the modENCODE project
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
    FlyMine - An integrated database for Drosophila genomics
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    Synonyms and Secondary IDs (25)
    Reported As
    Symbol Synonym
    Atg8a
    (Brooks et al., 2020, Redhai et al., 2020, Wang et al., 2020, Zhuang et al., 2020, Aparicio et al., 2019, Bhattacharjee et al., 2019, Bourouis et al., 2019, Bouska et al., 2019, Chatterjee et al., 2019, Gumeni et al., 2019, Guo et al., 2019, Jacomin et al., 2019, Johnson et al., 2019, Lieber et al., 2019, Loeffler, 2019, Lőrincz et al., 2019, Manola et al., 2019, Mao et al., 2019, Meltzer et al., 2019, Nakamura et al., 2019, Nilangekar et al., 2019, Rodriguez-Fernandez et al., 2019, Şentürk et al., 2019, Shimaji et al., 2019, Shukla et al., 2019, Sim et al., 2019, Su et al., 2019, Texada et al., 2019, Wu et al., 2019, Yan et al., 2019, Zhang et al., 2019, Zhu et al., 2019, Bardai et al., 2018, Billes et al., 2018, Gene Disruption Project members, 2018-, Issa et al., 2018, Liu et al., 2018, Lund et al., 2018, Monnier et al., 2018, O'Keefe and Denton, 2018, Richardson and Portela, 2018, Staats et al., 2018, Swevers et al., 2018, Tang et al., 2018, Zhang et al., 2018, Ding et al., 2017, Galluzzi et al., 2017, Hu et al., 2017.6.13, Kim et al., 2017, Kovács et al., 2017, Liu et al., 2017, Lo Piccolo et al., 2017, Lőrincz et al., 2017, Lőrincz et al., 2017, Manent et al., 2017, Nagy et al., 2017, Nandi et al., 2017, Neisch et al., 2017, Rana et al., 2017, Reynolds-Peterson et al., 2017, Sato and Sato, 2017, Sellin et al., 2017, Transgenic RNAi Project members, 2017-, Tusco et al., 2017, Yan et al., 2017, Barekat et al., 2016, Billes et al., 2016, Danielsen et al., 2016, Gene Disruption Project members, 2016-, Hegedűs et al., 2016, Kandul et al., 2016, Kinghorn et al., 2016, Kuleesha et al., 2016, Lőrincz et al., 2016, Maistrenko et al., 2016, Nakazawa et al., 2016, Pavel et al., 2016, Ratliff et al., 2016, Schmid et al., 2016, Tracy et al., 2016, Wang et al., 2016, Bargiela et al., 2015, Del Caño-Espinel et al., 2015, Jean et al., 2015, Jin et al., 2015, Kim et al., 2015, Kim et al., 2015, Menzies et al., 2015, Nagy et al., 2015, Park et al., 2015, Ratliff et al., 2015, Rojas-Ríos et al., 2015, Tsou et al., 2015, Xu et al., 2015, Zhang and Baehrecke, 2015, Banreti et al., 2014, Dodson et al., 2014, Issman-Zecharya and Schuldiner, 2014, Ling et al., 2014, Liu et al., 2014, Merlo et al., 2014, Morelli et al., 2014, Moy et al., 2014, Mulakkal et al., 2014, Nagy et al., 2014, Nagy et al., 2014, Nandi et al., 2014, Politi et al., 2014, Szatmári et al., 2014, Takáts et al., 2014, Ulgherait et al., 2014, Bai et al., 2013, Chang et al., 2013, Denton et al., 2013, Gupta et al., 2013, Kim et al., 2013, Knævelsrud et al., 2013, Liu et al., 2013, Lőw et al., 2013, Mockett and Nobles, 2013, Nagy et al., 2013, Owusu-Ansah et al., 2013, Shin et al., 2013, Shravage et al., 2013, Takáts et al., 2013, Tang et al., 2013, Bánréti et al., 2012, Denton et al., 2012, Eleftherianos and Castillo, 2012, Érdi et al., 2012, Garcia et al., 2012, Jimenez-Sanchez et al., 2012, Pircs et al., 2012, Barth et al., 2011, Bartlett et al., 2011, Napoletano et al., 2011, Ribeiro et al., 2011, Tang et al., 2011, Chang and Neufeld, 2010, Filimonenko et al., 2010, Francis et al., 2010, Kadandale et al., 2010, Kim et al., 2010, Li et al., 2010, McPhee et al., 2010, Nezis et al., 2010, Nisoli et al., 2010, Zirin and Perrimon, 2010, Arsham and Neufeld, 2009, Bass et al., 2009, Chang and Neufeld, 2009, Csikós et al., 2009, Denton et al., 2009, Köhler et al., 2009, Ling et al., 2009, Nezis et al., 2009, Shelly et al., 2009, Hou et al., 2008, Juhász et al., 2008, Kim et al., 2008, Simonsen et al., 2008, Botella et al., 2007, Goodliffe et al., 2007, Juhász et al., 2007, Juhász et al., 2007, Langille and Clark, 2007, Langille and Clark, 2007, Rusten et al., 2007, Scott et al., 2007, Scott et al., 2007, Simonsen et al., 2007, Zeitouni et al., 2007)
    BcDNA:LD05816
    Name Synonyms
    Autophagy-related 8a
    autophagy-related 8 protein
    autophagy-related 8a
    Secondary FlyBase IDs
    • FBgn0030229
    • FBgn0063168
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (340)