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Gene Dmel\Atg8a

General Information
Symbol	Dmel\Atg8a	Species	D. melanogaster
Name	Autophagy-specific gene 8a	Annotation symbol	CG32672
Feature type	protein_coding_gene	FlyBase ID	FBgn0052672
Gene Model Status	Current	Stock availability	8 publicly available
Also Known As	atg8, CG1534, LC3
Genomic Location
Chromosome (arm)	X	Recombination map
Cytogenetic map	9E6-9E7	Sequence location	X:10,658,911..10,662,368 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Autophagy-specific gene 8a is referred to in FlyBase by the symbol Dmel\Atg8a (CG32672, FBgn0052672). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: regulation of protein ubiquitination; determination of adult lifespan; macroautophagy; regulation of autophagy. 20 alleles are reported. The phenotype of these alleles is annotated with: embryonic/larval salivary gland. It has one annotated transcript and one annotated polypeptide. Protein features are: Autophagy protein Atg8 ubiquitin-like. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderately high expression. Peak expression observed at stages throughout embryogenesis, during late larval stages, at stages throughout the pupal period, in stages of adults of both sexes. Summary of FlyAtlas Anatomical Expression Data: High levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1639467_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Atg8a. Gene sequence location is X:10658911..10662368.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02	Controlled Vocabulary Terms amphisome
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 9E6-9E7   Limits computationally determined from genome sequence between P{EP}EP1645 and P{EP}Atg8aEP362&P{EP}CG1826EP1098
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Atg8a for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Low-frequency RNA-Seq exon junction(s) not annotated. Gene model reviewed during 5.47
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Atg8a-RA FBtr0071498  NM_167245   1211   121
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Atg8a-PA   FBpp0071427   14.4   121   9.44     NP_727447   AAF46617
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Autophagy protein Atg8 ubiquitin-like (IPR004241)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA246827   AA696091   AA698699   AE014298 AAF46617   AI135941   AI297572   AI945976   AI946825   AK284211   AQ025324   AW941604   AY122152 AAM52664   AY232215   BE662180   BE977793   BI168958   BI172119   BI173217   BI173372   BI213680   BI213689   BI215755   BI228198   BI228206   BI229727   BI236026   BI237641   BI240553   BI356728   BI364792   BI369066   BI369441   BI481630   BI483035   BI569100   BI569101   BI570461   BI580146   BI580997   BI580998   BI605211   BI605616   BI606145   BI620022   BI625841   BI629628   BI629913   BI639580   BI641022   BI641409   BP540587   BP540779   BP540780   BP540857   BP540858   BP542781   BP545417   BP545418   BP545759   BP546258   BP546452   BP546660   BP546850   BP547030   BP547226   BP547448   BP547464   BP547789   BP548152   BP548392   BP548643   BP548644   BP549131   BP549329   BP550896   BP554502   BP555466   BP555467   BP556159   BP556480   BP558447   BP558448   BP558861   BP558862   BP559663   BP559664   BP560109   BP560110   BP560385   BX012316   BX024259   BX028935   BX049446   BX057613   BX063659   CA803190   CA804976   CA806166   CA807798   CO152753   CO155331   CO156058   CO177694   CO182609   CO183400   CO183921   CO184257   CO185650   CO185725   CO185823   CO188340   CO189083   CO189085   CO190252   CO190588   CO190630   CO191347   CO193753   CO196526   CO269660   CO270923   CO273408   CO275697   CO276060   CO285506   CO286674   CO288735   CO291927   CO293887   CO294609   CO296181   CO298066   CO300096   CO300399   CO302663   CO303004   CO306021   CO306291   CO306648   CO307901   CO310217   CO313640   CO318470   CO320846   CO322628   CO324203   CO325215   CO328663   CO331694   CO332062   CO332641   CO332643   CO336356   CO338724   CO340467   DQ445535   EC058035   EC058690   EC060472   EC063861   EC063968   EC065052   EC065146   EC065607   EC069374   EC069486   EC071226   EC074535   EC076645   EC077994   EC079283   EC080267   EC081032   EC198603   EC200403   EC200973   EC200976   EC205753   EC206279   EC208798   EC211269   EC214654   EC215618   EC216038   EC216135   EC217016   EC217017   EC217018   EC217396   EC218203   EC221055   EC221865   EC222602   EC223059   EC225108   EC226232   EC228036   EC228149   EC232725   EC235166   EC239553   EC239677   EC240455   EC241839   EC246414   EC249150   EC250132   EC251166   EC259272   EC261427   EC262915   EC264440   EC264458   EC267574   EL880386   EL880387   EV585659   EV585984   EV587779   EV593429   GH748039   GH752267   GH757833   GH760331   GH761316   GH772646   GH785294   GH785910   GH788226   GH791284   GH793238   GH793643   GH797721   GH799023   GH801616   GH804759   GH807458   GH807513   GH807529   GH809901   GH810302   GH815091   GH815368   GH820574   GH826614   GH828970   GH829528   GH830353   GH832943   GH843233   GH845550   GH846516   GH847328   GH850992   GH853974   GH855513   GH857613   GH864230   GH866949   GH873584   GH875420   GH877444   GH878321   GH879871   GH890029   GH895572   GH898862   GH899628   GH899856   GH903014   GH908187   GH914384   GH915941   GH916276   GH924510   GH924755   GH925232   GH925520   GH930297
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9W2S2
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
amphisome inferred from direct assay (Wong et al., 2012) autophagic vacuole inferred from direct assay (Scott et al., 2004, Wong et al., 2012)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: High levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    1336.5 Larval Midgut    3403.5 Larval Hindgut    2499.1 Larval Malpighian Tubules    3522.4 Larval Fat Body    3428.9 Larval Salivary Gland    2714.1 Larval Trachea    2379.75 Larval Carcass    2514.675 Adult Head    2328.8 Adult Eye    3755.525 Adult Brain    2032.6 Adult Thoracic-Abdominal Ganglion    2483.7 Adult Crop    3036.6 Adult Midgut    2452.1 Adult Hindgut    2712 Adult Malpighian Tubules    2259.9 Adult Fat Body    2906.4 Adult Salivary Gland    6446.9 Adult Heart    3715.325 Adult VirginFemale Spermatheca    2616.4 Adult InseminatedFemale Spermatheca    2349.1 Adult Ovary    1078 Adult Testis    824.3 Adult Male Accessory Gland    4099.9 Adult Carcass    2816.7 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1336.5) Larval Midgut  (3403.5) Larval Hindgut  (2499.1) Larval Malpighian Tubules  (3522.4) Larval Fat Body  (3428.9) Larval Salivary Gland  (2714.1) Larval Trachea  (2379.75) Larval Carcass  (2514.675) Adult Head  (2328.8) Adult Eye  (3755.525) Adult Brain  (2032.6) Adult Thoracic-Abdominal Ganglion  (2483.7) Adult Crop  (3036.6) Adult Midgut  (2452.1) Adult Hindgut  (2712) Adult Malpighian Tubules  (2259.9) Adult Fat Body  (2906.4) Adult Salivary Gland  (6446.9) Adult Heart  (3715.325) Adult VirginFemale Spermatheca  (2616.4) Adult InseminatedFemale Spermatheca  (2349.1) Adult Ovary  (1078) Adult Testis  (824.3) Adult Male Accessory Gland  (4099.9) Adult Carcass  (2816.7) Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  (1336.5) Larval Midgut  (3403.5) Larval Hindgut  (2499.1) Larval Malpighian Tubules  (3522.4) Larval Fat Body  (3428.9) Larval Salivary Gland  (2714.1) Larval Trachea  (2379.75) Larval Carcass  (2514.675) Adult Head  (2328.8) Adult Eye  (3755.525) Adult Brain  (2032.6) Adult Thoracic-Abdominal Ganglion  (2483.7) Adult Crop  (3036.6) Adult Midgut  (2452.1) Adult Hindgut  (2712) Adult Malpighian Tubules  (2259.9) Adult Fat Body  (2906.4) Adult Salivary Gland  (6446.9) Adult Heart  (3715.325) Adult VirginFemale Spermatheca  (2616.4) Adult InseminatedFemale Spermatheca  (2349.1) Adult Ovary  1078 Adult Testis    824.3 Adult Male Accessory Gland  (4099.9) Adult Carcass  (2816.7) Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1336.5 Larval Midgut    3403.5 Larval Hindgut    2499.1 Larval Malpighian Tubules    3522.4 Larval Fat Body    3428.9 Larval Salivary Gland    2714.1 Larval Trachea    2379.75 Larval Carcass    2514.675 Adult Head    2328.8 Adult Eye    3755.525 Adult Brain    2032.6 Adult Thoracic-Abdominal Ganglion    2483.7 Adult Crop    3036.6 Adult Midgut    2452.1 Adult Hindgut    2712 Adult Malpighian Tubules    2259.9 Adult Fat Body    2906.4 Adult Salivary Gland    6446.9 Adult Heart    3715.325 Adult VirginFemale Spermatheca    2616.4 Adult InseminatedFemale Spermatheca    2349.1 Adult Ovary    1078 Adult Testis    824.3 Adult Male Accessory Gland    4099.9 Adult Carcass    2816.7 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    1336.5 Larval Midgut    3403.5 Larval Hindgut    2499.1 Larval Malpighian Tubules    3522.4 Larval Fat Body    3428.9 Larval Salivary Gland    2714.1 Larval Trachea    2379.75 Larval Carcass    2514.675 Adult Head    2328.8 Adult Eye    3755.525 Adult Brain    2032.6 Adult Thoracic-Abdominal Ganglion    2483.7 Adult Crop    3036.6 Adult Midgut    2452.1 Adult Hindgut    2712 Adult Malpighian Tubules    2259.9 Adult Fat Body    2906.4 Adult Salivary Gland    6446.9 Adult Heart    3715.325 Adult VirginFemale Spermatheca    2616.4 Adult InseminatedFemale Spermatheca    2349.1 Adult Ovary    1078 Adult Testis    824.3 Adult Male Accessory Gland    4099.9 Adult Carcass    2816.7 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1336.5) Larval Midgut  (3403.5) Larval Hindgut  (2499.1) Larval Malpighian Tubules  (3522.4) Larval Fat Body  (3428.9) Larval Salivary Gland  (2714.1) Larval Trachea  (2379.75) Larval Carcass  (2514.675) Adult Head  (2328.8) Adult Eye  (3755.525) Adult Brain  (2032.6) Adult Thoracic-Abdominal Ganglion  (2483.7) Adult Crop  (3036.6) Adult Midgut  (2452.1) Adult Hindgut  (2712) Adult Malpighian Tubules  (2259.9) Adult Fat Body  (2906.4) Adult Salivary Gland  (6446.9) Adult Heart  (3715.325) Adult VirginFemale Spermatheca  (2616.4) Adult InseminatedFemale Spermatheca  (2349.1) Adult Ovary  (1078) Adult Testis  (824.3) Adult Male Accessory Gland  (4099.9) Adult Carcass  (2816.7) Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  1336.5 Larval Midgut  (3403.5) Larval Hindgut  (2499.1) Larval Malpighian Tubules  (3522.4) Larval Fat Body  (3428.9) Larval Salivary Gland  (2714.1) Larval Trachea  (2379.75) Larval Carcass  (2514.675) Adult Head  (2328.8) Adult Eye  (3755.525) Adult Brain  (2032.6) Adult Thoracic-Abdominal Ganglion  (2483.7) Adult Crop  (3036.6) Adult Midgut  (2452.1) Adult Hindgut  (2712) Adult Malpighian Tubules  (2259.9) Adult Fat Body  (2906.4) Adult Salivary Gland  (6446.9) Adult Heart  (3715.325) Adult VirginFemale Spermatheca  (2616.4) Adult InseminatedFemale Spermatheca  (2349.1) Adult Ovary  1078 Adult Testis    824.3 Adult Male Accessory Gland  (4099.9) Adult Carcass  (2816.7) Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1336.5 Larval Midgut    3403.5 Larval Hindgut    2499.1 Larval Malpighian Tubules    3522.4 Larval Fat Body    3428.9 Larval Salivary Gland    2714.1 Larval Trachea    2379.75 Larval Carcass    2514.675 Adult Head    2328.8 Adult Eye    3755.525 Adult Brain    2032.6 Adult Thoracic-Abdominal Ganglion    2483.7 Adult Crop    3036.6 Adult Midgut    2452.1 Adult Hindgut    2712 Adult Malpighian Tubules    2259.9 Adult Fat Body    2906.4 Adult Salivary Gland    6446.9 Adult Heart    3715.325 Adult VirginFemale Spermatheca    2616.4 Adult InseminatedFemale Spermatheca    2349.1 Adult Ovary    1078 Adult Testis    824.3 Adult Male Accessory Gland    4099.9 Adult Carcass    2816.7 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    2328.8   NA  Eye    3755.525   NA  Brain    2032.6   1336.5  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    2483.7   NA  Crop    3036.6   3403.5  Midgut    2452.1   2499.1  Hindgut    2712   3522.4  Malpighian Tubules    2259.9   3428.9  Fat Body    2906.4   2714.1  Salivary Gland    6446.9   NA  Heart    3715.325   2379.75  Trachea    NA   NA  VirginFemale Spermatheca    2616.4   NA  InseminatedFemale Spermatheca    2349.1   NA  Ovary    1078   NA  Testis    824.3   NA  Male Accessory Gland    4099.9   2514.675  Carcass    2816.7 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    212 central nervous system, larvae L3    203 central nervous system, pupae P8    212 head, virgin 1-day female    290 head, virgin 4-day female    290 head, virgin 20-day female    231 head, mated 1-day female    329 head, mated 4-day female    283 head, mated 20-day female    251 head, mated 1-day male    314 head, mated 4-day male    394 head, mated 20-day male    273 salivary gland, larvae L3 wandering    174 salivary gland, white prepupae    686 digestive system, larvae L3 wandering    1242 digestive system, 1-day adult    349 digestive system, 4-day adult    278 digestive system, 20-day adult    271 fat body, larvae L3 wandering    257 fat body, white prepupae    336 fat body, pupae P8    283 carcass, larvae L3 wandering    272 carcass, 1-day adult    435 carcass, 4-day adult    328 carcass, 20-day adult    299 ovary, virgin 4-day female    132 ovary, mated 4-day female    110 testis, mated 4-day male    85 accessory gland, mated 4-day male    260 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (212) central nervous system, larvae L3  (203) central nervous system, pupae P8  (212) head, virgin 1-day female  (290) head, virgin 4-day female  (290) head, virgin 20-day female  (231) head, mated 1-day female  (329) head, mated 4-day female  (283) head, mated 20-day female  (251) head, mated 1-day male  (314) head, mated 4-day male  (394) head, mated 20-day male  (273) salivary gland, larvae L3 wandering  (174) salivary gland, white prepupae  (686) digestive system, larvae L3 wandering  (1242) digestive system, 1-day adult  (349) digestive system, 4-day adult  (278) digestive system, 20-day adult  (271) fat body, larvae L3 wandering  (257) fat body, white prepupae  (336) fat body, pupae P8  (283) carcass, larvae L3 wandering  (272) carcass, 1-day adult  (435) carcass, 4-day adult  (328) carcass, 20-day adult  (299) ovary, virgin 4-day female  (132) ovary, mated 4-day female  (110) testis, mated 4-day male  (85) accessory gland, mated 4-day male  (260) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (212) central nervous system, larvae L3  (203) central nervous system, pupae P8  (212) head, virgin 1-day female  (290) head, virgin 4-day female  (290) head, virgin 20-day female  (231) head, mated 1-day female  (329) head, mated 4-day female  (283) head, mated 20-day female  (251) head, mated 1-day male  (314) head, mated 4-day male  (394) head, mated 20-day male  (273) salivary gland, larvae L3 wandering  (174) salivary gland, white prepupae  (686) digestive system, larvae L3 wandering  (1242) digestive system, 1-day adult  (349) digestive system, 4-day adult  (278) digestive system, 20-day adult  (271) fat body, larvae L3 wandering  (257) fat body, white prepupae  (336) fat body, pupae P8  (283) carcass, larvae L3 wandering  (272) carcass, 1-day adult  (435) carcass, 4-day adult  (328) carcass, 20-day adult  (299) ovary, virgin 4-day female  (132) ovary, mated 4-day female  (110) testis, mated 4-day male    85 accessory gland, mated 4-day male  (260) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    212 central nervous system, larvae L3    203 central nervous system, pupae P8    212 head, virgin 1-day female    290 head, virgin 4-day female    290 head, virgin 20-day female    231 head, mated 1-day female    329 head, mated 4-day female    283 head, mated 20-day female    251 head, mated 1-day male    314 head, mated 4-day male    394 head, mated 20-day male    273 salivary gland, larvae L3 wandering    174 salivary gland, white prepupae    686 digestive system, larvae L3 wandering    1242 digestive system, 1-day adult    349 digestive system, 4-day adult    278 digestive system, 20-day adult    271 fat body, larvae L3 wandering    257 fat body, white prepupae    336 fat body, pupae P8    283 carcass, larvae L3 wandering    272 carcass, 1-day adult    435 carcass, 4-day adult    328 carcass, 20-day adult    299 ovary, virgin 4-day female    132 ovary, mated 4-day female    110 testis, mated 4-day male    85 accessory gland, mated 4-day male    260 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    212 central nervous system, larvae L3    203 central nervous system, pupae P8    212 head, virgin 1-day female    290 head, virgin 4-day female    290 head, virgin 20-day female    231 head, mated 1-day female    329 head, mated 4-day female    283 head, mated 20-day female    251 head, mated 1-day male    314 head, mated 4-day male    394 head, mated 20-day male    273 salivary gland, larvae L3 wandering    174 salivary gland, white prepupae    686 digestive system, larvae L3 wandering    1242 digestive system, 1-day adult    349 digestive system, 4-day adult    278 digestive system, 20-day adult    271 fat body, larvae L3 wandering    257 fat body, white prepupae    336 fat body, pupae P8    283 carcass, larvae L3 wandering    272 carcass, 1-day adult    435 carcass, 4-day adult    328 carcass, 20-day adult    299 ovary, virgin 4-day female    132 ovary, mated 4-day female    110 testis, mated 4-day male    85 accessory gland, mated 4-day male    260 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (212) central nervous system, larvae L3  (203) central nervous system, pupae P8  (212) head, virgin 1-day female  (290) head, virgin 4-day female  (290) head, virgin 20-day female  (231) head, mated 1-day female  (329) head, mated 4-day female  (283) head, mated 20-day female  (251) head, mated 1-day male  (314) head, mated 4-day male  (394) head, mated 20-day male  (273) salivary gland, larvae L3 wandering  (174) salivary gland, white prepupae  (686) digestive system, larvae L3 wandering  (1242) digestive system, 1-day adult  (349) digestive system, 4-day adult  (278) digestive system, 20-day adult  (271) fat body, larvae L3 wandering  (257) fat body, white prepupae  (336) fat body, pupae P8  (283) carcass, larvae L3 wandering  (272) carcass, 1-day adult  (435) carcass, 4-day adult  (328) carcass, 20-day adult  (299) ovary, virgin 4-day female  (132) ovary, mated 4-day female  (110) testis, mated 4-day male  (85) accessory gland, mated 4-day male  (260) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (212) central nervous system, larvae L3  (203) central nervous system, pupae P8  (212) head, virgin 1-day female  (290) head, virgin 4-day female  (290) head, virgin 20-day female  (231) head, mated 1-day female  (329) head, mated 4-day female  (283) head, mated 20-day female  (251) head, mated 1-day male  (314) head, mated 4-day male  (394) head, mated 20-day male  (273) salivary gland, larvae L3 wandering  (174) salivary gland, white prepupae  (686) digestive system, larvae L3 wandering  (1242) digestive system, 1-day adult  (349) digestive system, 4-day adult  (278) digestive system, 20-day adult  (271) fat body, larvae L3 wandering  (257) fat body, white prepupae  (336) fat body, pupae P8  (283) carcass, larvae L3 wandering  (272) carcass, 1-day adult  (435) carcass, 4-day adult  (328) carcass, 20-day adult  (299) ovary, virgin 4-day female  132 ovary, mated 4-day female  110 testis, mated 4-day male    85 accessory gland, mated 4-day male  (260) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    212 central nervous system, larvae L3    203 central nervous system, pupae P8    212 head, virgin 1-day female    290 head, virgin 4-day female    290 head, virgin 20-day female    231 head, mated 1-day female    329 head, mated 4-day female    283 head, mated 20-day female    251 head, mated 1-day male    314 head, mated 4-day male    394 head, mated 20-day male    273 salivary gland, larvae L3 wandering    174 salivary gland, white prepupae    686 digestive system, larvae L3 wandering    1242 digestive system, 1-day adult    349 digestive system, 4-day adult    278 digestive system, 20-day adult    271 fat body, larvae L3 wandering    257 fat body, white prepupae    336 fat body, pupae P8    283 carcass, larvae L3 wandering    272 carcass, 1-day adult    435 carcass, 4-day adult    328 carcass, 20-day adult    299 ovary, virgin 4-day female    132 ovary, mated 4-day female    110 testis, mated 4-day male    85 accessory gland, mated 4-day male    260 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderately high expression. Peak expression observed at stages throughout embryogenesis, during late larval stages, at stages throughout the pupal period, in stages of adults of both sexes. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    77 embryo 02-04hr    151 embryo 04-06hr    272 embryo 06-08hr    197 embryo 08-10hr    215 embryo 10-12hr    184 embryo 12-14hr    216 embryo 14-16hr    180 embryo 16-18hr    183 embryo 18-20hr    151 embryo 20-22hr    161 embryo 22-24hr    226 larva L1    79 larva L2    50 larva L3 12hr old    77 larva L3 puffstage 1-2    204 larva L3 puffstage 3-6    307 larva L3 puffstage 7-9    436 white prepupae new    496 white prepupae 12hr    335 white prepupae 24hr    235 pupae 2d postWPP    177 pupae 3d postWPP    115 pupae 4d postWPP    187 adult male 01day    251 adult male 05day    214 adult male 30day    164 adult female 01day    178 adult female 05day    133 adult female 30day    114 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (77) embryo 02-04hr  (151) embryo 04-06hr  (272) embryo 06-08hr  (197) embryo 08-10hr  (215) embryo 10-12hr  (184) embryo 12-14hr  (216) embryo 14-16hr  (180) embryo 16-18hr  (183) embryo 18-20hr  (151) embryo 20-22hr  (161) embryo 22-24hr  (226) larva L1  (79) larva L2  (50) larva L3 12hr old  (77) larva L3 puffstage 1-2  (204) larva L3 puffstage 3-6  (307) larva L3 puffstage 7-9  (436) white prepupae new  (496) white prepupae 12hr  (335) white prepupae 24hr  (235) pupae 2d postWPP  (177) pupae 3d postWPP  (115) pupae 4d postWPP  (187) adult male 01day  (251) adult male 05day  (214) adult male 30day  (164) adult female 01day  (178) adult female 05day  (133) adult female 30day  (114) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    77 embryo 02-04hr  (151) embryo 04-06hr  (272) embryo 06-08hr  (197) embryo 08-10hr  (215) embryo 10-12hr  (184) embryo 12-14hr  (216) embryo 14-16hr  (180) embryo 16-18hr  (183) embryo 18-20hr  (151) embryo 20-22hr  (161) embryo 22-24hr  (226) larva L1    79 larva L2    50 larva L3 12hr old    77 larva L3 puffstage 1-2  (204) larva L3 puffstage 3-6  (307) larva L3 puffstage 7-9  (436) white prepupae new  (496) white prepupae 12hr  (335) white prepupae 24hr  (235) pupae 2d postWPP  (177) pupae 3d postWPP  (115) pupae 4d postWPP  (187) adult male 01day  (251) adult male 05day  (214) adult male 30day  (164) adult female 01day  (178) adult female 05day  (133) adult female 30day  (114) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    77 embryo 02-04hr    151 embryo 04-06hr    272 embryo 06-08hr    197 embryo 08-10hr    215 embryo 10-12hr    184 embryo 12-14hr    216 embryo 14-16hr    180 embryo 16-18hr    183 embryo 18-20hr    151 embryo 20-22hr    161 embryo 22-24hr    226 larva L1    79 larva L2    50 larva L3 12hr old    77 larva L3 puffstage 1-2    204 larva L3 puffstage 3-6    307 larva L3 puffstage 7-9    436 white prepupae new    496 white prepupae 12hr    335 white prepupae 24hr    235 pupae 2d postWPP    177 pupae 3d postWPP    115 pupae 4d postWPP    187 adult male 01day    251 adult male 05day    214 adult male 30day    164 adult female 01day    178 adult female 05day    133 adult female 30day    114 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    77 embryo 02-04hr    151 embryo 04-06hr    272 embryo 06-08hr    197 embryo 08-10hr    215 embryo 10-12hr    184 embryo 12-14hr    216 embryo 14-16hr    180 embryo 16-18hr    183 embryo 18-20hr    151 embryo 20-22hr    161 embryo 22-24hr    226 larva L1    79 larva L2    50 larva L3 12hr old    77 larva L3 puffstage 1-2    204 larva L3 puffstage 3-6    307 larva L3 puffstage 7-9    436 white prepupae new    496 white prepupae 12hr    335 white prepupae 24hr    235 pupae 2d postWPP    177 pupae 3d postWPP    115 pupae 4d postWPP    187 adult male 01day    251 adult male 05day    214 adult male 30day    164 adult female 01day    178 adult female 05day    133 adult female 30day    114 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (77) embryo 02-04hr  (151) embryo 04-06hr  (272) embryo 06-08hr  (197) embryo 08-10hr  (215) embryo 10-12hr  (184) embryo 12-14hr  (216) embryo 14-16hr  (180) embryo 16-18hr  (183) embryo 18-20hr  (151) embryo 20-22hr  (161) embryo 22-24hr  (226) larva L1  (79) larva L2  (50) larva L3 12hr old  (77) larva L3 puffstage 1-2  (204) larva L3 puffstage 3-6  (307) larva L3 puffstage 7-9  (436) white prepupae new  (496) white prepupae 12hr  (335) white prepupae 24hr  (235) pupae 2d postWPP  (177) pupae 3d postWPP  (115) pupae 4d postWPP  (187) adult male 01day  (251) adult male 05day  (214) adult male 30day  (164) adult female 01day  (178) adult female 05day  (133) adult female 30day  (114) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    77 embryo 02-04hr  (151) embryo 04-06hr  (272) embryo 06-08hr  (197) embryo 08-10hr  (215) embryo 10-12hr  (184) embryo 12-14hr  (216) embryo 14-16hr  (180) embryo 16-18hr  (183) embryo 18-20hr  (151) embryo 20-22hr  (161) embryo 22-24hr  (226) larva L1    79 larva L2    50 larva L3 12hr old    77 larva L3 puffstage 1-2  (204) larva L3 puffstage 3-6  (307) larva L3 puffstage 7-9  (436) white prepupae new  (496) white prepupae 12hr  (335) white prepupae 24hr  (235) pupae 2d postWPP  (177) pupae 3d postWPP  115 pupae 4d postWPP  (187) adult male 01day  (251) adult male 05day  (214) adult male 30day  (164) adult female 01day  (178) adult female 05day  133 adult female 30day  114 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    77 embryo 02-04hr    151 embryo 04-06hr    272 embryo 06-08hr    197 embryo 08-10hr    215 embryo 10-12hr    184 embryo 12-14hr    216 embryo 14-16hr    180 embryo 16-18hr    183 embryo 18-20hr    151 embryo 20-22hr    161 embryo 22-24hr    226 larva L1    79 larva L2    50 larva L3 12hr old    77 larva L3 puffstage 1-2    204 larva L3 puffstage 3-6    307 larva L3 puffstage 7-9    436 white prepupae new    496 white prepupae 12hr    335 white prepupae 24hr    235 pupae 2d postWPP    177 pupae 3d postWPP    115 pupae 4d postWPP    187 adult male 01day    251 adult male 05day    214 adult male 30day    164 adult female 01day    178 adult female 05day    133 adult female 30day    114 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    257 Schneider line 2 Sg4    108 embryonic 1182-4H    141 embryonic GM2    345 embryonic Kc167    691 embryonic S1    205 embryonic S3    126 leg disc CME L1    86 wing disc CME-W2    283 wing disc ML-DmD8    182 wing disc ML-DmD9    305 wing disc ML-DmD16-c3    163 wing disc ML-DmD21    97 wing disc ML-DmD32    183 haltere disc ML-DmD17-c3    174 eye-antennal disc ML-DmD11    183 antennal disc ML-DmD20-c5    159 mixed discs ML-DmD4-c1    191 CNS ML-DmBG1-c1    234 CNS ML-DmBG2-c2    223 tumorous blood cells mbn2    542 ovary fGS/OSS    193 ovary OSC    342 ovary OSS    115 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (257) Schneider line 2 Sg4  (108) embryonic 1182-4H  (141) embryonic GM2  (345) embryonic Kc167  (691) embryonic S1  (205) embryonic S3  (126) leg disc CME L1  (86) wing disc CME-W2  (283) wing disc ML-DmD8  (182) wing disc ML-DmD9  (305) wing disc ML-DmD16-c3  (163) wing disc ML-DmD21  (97) wing disc ML-DmD32  (183) haltere disc ML-DmD17-c3  (174) eye-antennal disc ML-DmD11  (183) antennal disc ML-DmD20-c5  (159) mixed discs ML-DmD4-c1  (191) CNS ML-DmBG1-c1  (234) CNS ML-DmBG2-c2  (223) tumorous blood cells mbn2  (542) ovary fGS/OSS  (193) ovary OSC  (342) ovary OSS  (115) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (257) Schneider line 2 Sg4  (108) embryonic 1182-4H  (141) embryonic GM2  (345) embryonic Kc167  (691) embryonic S1  (205) embryonic S3  (126) leg disc CME L1    86 wing disc CME-W2  (283) wing disc ML-DmD8  (182) wing disc ML-DmD9  (305) wing disc ML-DmD16-c3  (163) wing disc ML-DmD21    97 wing disc ML-DmD32  (183) haltere disc ML-DmD17-c3  (174) eye-antennal disc ML-DmD11  (183) antennal disc ML-DmD20-c5  (159) mixed discs ML-DmD4-c1  (191) CNS ML-DmBG1-c1  (234) CNS ML-DmBG2-c2  (223) tumorous blood cells mbn2  (542) ovary fGS/OSS  (193) ovary OSC  (342) ovary OSS  (115) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    257 Schneider line 2 Sg4    108 embryonic 1182-4H    141 embryonic GM2    345 embryonic Kc167    691 embryonic S1    205 embryonic S3    126 leg disc CME L1    86 wing disc CME-W2    283 wing disc ML-DmD8    182 wing disc ML-DmD9    305 wing disc ML-DmD16-c3    163 wing disc ML-DmD21    97 wing disc ML-DmD32    183 haltere disc ML-DmD17-c3    174 eye-antennal disc ML-DmD11    183 antennal disc ML-DmD20-c5    159 mixed discs ML-DmD4-c1    191 CNS ML-DmBG1-c1    234 CNS ML-DmBG2-c2    223 tumorous blood cells mbn2    542 ovary fGS/OSS    193 ovary OSC    342 ovary OSS    115 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    257 Schneider line 2 Sg4    108 embryonic 1182-4H    141 embryonic GM2    345 embryonic Kc167    691 embryonic S1    205 embryonic S3    126 leg disc CME L1    86 wing disc CME-W2    283 wing disc ML-DmD8    182 wing disc ML-DmD9    305 wing disc ML-DmD16-c3    163 wing disc ML-DmD21    97 wing disc ML-DmD32    183 haltere disc ML-DmD17-c3    174 eye-antennal disc ML-DmD11    183 antennal disc ML-DmD20-c5    159 mixed discs ML-DmD4-c1    191 CNS ML-DmBG1-c1    234 CNS ML-DmBG2-c2    223 tumorous blood cells mbn2    542 ovary fGS/OSS    193 ovary OSC    342 ovary OSS    115 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (257) Schneider line 2 Sg4  (108) embryonic 1182-4H  (141) embryonic GM2  (345) embryonic Kc167  (691) embryonic S1  (205) embryonic S3  (126) leg disc CME L1  (86) wing disc CME-W2  (283) wing disc ML-DmD8  (182) wing disc ML-DmD9  (305) wing disc ML-DmD16-c3  (163) wing disc ML-DmD21  (97) wing disc ML-DmD32  (183) haltere disc ML-DmD17-c3  (174) eye-antennal disc ML-DmD11  (183) antennal disc ML-DmD20-c5  (159) mixed discs ML-DmD4-c1  (191) CNS ML-DmBG1-c1  (234) CNS ML-DmBG2-c2  (223) tumorous blood cells mbn2  (542) ovary fGS/OSS  (193) ovary OSC  (342) ovary OSS  (115) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (257) Schneider line 2 Sg4  108 embryonic 1182-4H  141 embryonic GM2  (345) embryonic Kc167  (691) embryonic S1  (205) embryonic S3  126 leg disc CME L1    86 wing disc CME-W2  (283) wing disc ML-DmD8  (182) wing disc ML-DmD9  (305) wing disc ML-DmD16-c3  (163) wing disc ML-DmD21    97 wing disc ML-DmD32  (183) haltere disc ML-DmD17-c3  (174) eye-antennal disc ML-DmD11  (183) antennal disc ML-DmD20-c5  (159) mixed discs ML-DmD4-c1  (191) CNS ML-DmBG1-c1  (234) CNS ML-DmBG2-c2  (223) tumorous blood cells mbn2  (542) ovary fGS/OSS  (193) ovary OSC  (342) ovary OSS  115 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    257 Schneider line 2 Sg4    108 embryonic 1182-4H    141 embryonic GM2    345 embryonic Kc167    691 embryonic S1    205 embryonic S3    126 leg disc CME L1    86 wing disc CME-W2    283 wing disc ML-DmD8    182 wing disc ML-DmD9    305 wing disc ML-DmD16-c3    163 wing disc ML-DmD21    97 wing disc ML-DmD32    183 haltere disc ML-DmD17-c3    174 eye-antennal disc ML-DmD11    183 antennal disc ML-DmD20-c5    159 mixed discs ML-DmD4-c1    191 CNS ML-DmBG1-c1    234 CNS ML-DmBG2-c2    223 tumorous blood cells mbn2    542 ovary fGS/OSS    193 ovary OSC    342 ovary OSS    115 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    102 cold shock, 4-day adult    77 heat shock, 4-day adult    163 Cadmium 50 mM 6 hrs, larvae L3    159 Cadmium 50 mM 12 hrs, larvae L3    562 Cadmium 50 mM 48 hrs, 4-day adult    229 Cadmium 100 mM 48 hrs, 4-day adult    331 Copper 0.5 mM 12 hrs, larvae L3    57 Copper 15 mM 48 hrs, 4-day adult    911 Zinc 5 mM 12 hrs, larvae L3    67 Zinc 4.5 mM 48 hrs, 4-day adult    624 Ethanol 2.5% 3 hrs, larvae L3    167 Ethanol 5% 3 hrs, larvae L3    439 Ethanol 10% 3 hrs, larvae L3    155 Caffeine 1.5 mg/ml 4 hrs, larvae L3    549 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    353 Caffeine 25 mg/ml 48 hrs, 4-day adult    148 Paraquat 5 mM 48 hrs, 4-day adult    245 Paraquat 10 mM 48 hrs, 4-day adult    245 Rotenone 2 μg 12 hrs, larvae L3    226 Rotenone 8 μg 12 hrs, larvae L3    136 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (102) cold shock, 4-day adult  (77) heat shock, 4-day adult  (163) Cadmium 50 mM 6 hrs, larvae L3  (159) Cadmium 50 mM 12 hrs, larvae L3  (562) Cadmium 50 mM 48 hrs, 4-day adult  (229) Cadmium 100 mM 48 hrs, 4-day adult  (331) Copper 0.5 mM 12 hrs, larvae L3  (57) Copper 15 mM 48 hrs, 4-day adult  (911) Zinc 5 mM 12 hrs, larvae L3  (67) Zinc 4.5 mM 48 hrs, 4-day adult  (624) Ethanol 2.5% 3 hrs, larvae L3  (167) Ethanol 5% 3 hrs, larvae L3  (439) Ethanol 10% 3 hrs, larvae L3  (155) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (549) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (353) Caffeine 25 mg/ml 48 hrs, 4-day adult  (148) Paraquat 5 mM 48 hrs, 4-day adult  (245) Paraquat 10 mM 48 hrs, 4-day adult  (245) Rotenone 2 μg 12 hrs, larvae L3  (226) Rotenone 8 μg 12 hrs, larvae L3  (136) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult  102 cold shock, 4-day adult    77 heat shock, 4-day adult  (163) Cadmium 50 mM 6 hrs, larvae L3  (159) Cadmium 50 mM 12 hrs, larvae L3  (562) Cadmium 50 mM 48 hrs, 4-day adult  (229) Cadmium 100 mM 48 hrs, 4-day adult  (331) Copper 0.5 mM 12 hrs, larvae L3    57 Copper 15 mM 48 hrs, 4-day adult  (911) Zinc 5 mM 12 hrs, larvae L3    67 Zinc 4.5 mM 48 hrs, 4-day adult  (624) Ethanol 2.5% 3 hrs, larvae L3  (167) Ethanol 5% 3 hrs, larvae L3  (439) Ethanol 10% 3 hrs, larvae L3  (155) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (549) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (353) Caffeine 25 mg/ml 48 hrs, 4-day adult  (148) Paraquat 5 mM 48 hrs, 4-day adult  (245) Paraquat 10 mM 48 hrs, 4-day adult  (245) Rotenone 2 μg 12 hrs, larvae L3  (226) Rotenone 8 μg 12 hrs, larvae L3  (136) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    102 cold shock, 4-day adult    77 heat shock, 4-day adult    163 Cadmium 50 mM 6 hrs, larvae L3    159 Cadmium 50 mM 12 hrs, larvae L3    562 Cadmium 50 mM 48 hrs, 4-day adult    229 Cadmium 100 mM 48 hrs, 4-day adult    331 Copper 0.5 mM 12 hrs, larvae L3    57 Copper 15 mM 48 hrs, 4-day adult    911 Zinc 5 mM 12 hrs, larvae L3    67 Zinc 4.5 mM 48 hrs, 4-day adult    624 Ethanol 2.5% 3 hrs, larvae L3    167 Ethanol 5% 3 hrs, larvae L3    439 Ethanol 10% 3 hrs, larvae L3    155 Caffeine 1.5 mg/ml 4 hrs, larvae L3    549 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    353 Caffeine 25 mg/ml 48 hrs, 4-day adult    148 Paraquat 5 mM 48 hrs, 4-day adult    245 Paraquat 10 mM 48 hrs, 4-day adult    245 Rotenone 2 μg 12 hrs, larvae L3    226 Rotenone 8 μg 12 hrs, larvae L3    136 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    102 cold shock, 4-day adult    77 heat shock, 4-day adult    163 Cadmium 50 mM 6 hrs, larvae L3    159 Cadmium 50 mM 12 hrs, larvae L3    562 Cadmium 50 mM 48 hrs, 4-day adult    229 Cadmium 100 mM 48 hrs, 4-day adult    331 Copper 0.5 mM 12 hrs, larvae L3    57 Copper 15 mM 48 hrs, 4-day adult    911 Zinc 5 mM 12 hrs, larvae L3    67 Zinc 4.5 mM 48 hrs, 4-day adult    624 Ethanol 2.5% 3 hrs, larvae L3    167 Ethanol 5% 3 hrs, larvae L3    439 Ethanol 10% 3 hrs, larvae L3    155 Caffeine 1.5 mg/ml 4 hrs, larvae L3    549 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    353 Caffeine 25 mg/ml 48 hrs, 4-day adult    148 Paraquat 5 mM 48 hrs, 4-day adult    245 Paraquat 10 mM 48 hrs, 4-day adult    245 Rotenone 2 μg 12 hrs, larvae L3    226 Rotenone 8 μg 12 hrs, larvae L3    136 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (102) cold shock, 4-day adult  (77) heat shock, 4-day adult  (163) Cadmium 50 mM 6 hrs, larvae L3  (159) Cadmium 50 mM 12 hrs, larvae L3  (562) Cadmium 50 mM 48 hrs, 4-day adult  (229) Cadmium 100 mM 48 hrs, 4-day adult  (331) Copper 0.5 mM 12 hrs, larvae L3  (57) Copper 15 mM 48 hrs, 4-day adult  (911) Zinc 5 mM 12 hrs, larvae L3  (67) Zinc 4.5 mM 48 hrs, 4-day adult  (624) Ethanol 2.5% 3 hrs, larvae L3  (167) Ethanol 5% 3 hrs, larvae L3  (439) Ethanol 10% 3 hrs, larvae L3  (155) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (549) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (353) Caffeine 25 mg/ml 48 hrs, 4-day adult  (148) Paraquat 5 mM 48 hrs, 4-day adult  (245) Paraquat 10 mM 48 hrs, 4-day adult  (245) Rotenone 2 μg 12 hrs, larvae L3  (226) Rotenone 8 μg 12 hrs, larvae L3  (136) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult  102 cold shock, 4-day adult    77 heat shock, 4-day adult  (163) Cadmium 50 mM 6 hrs, larvae L3  (159) Cadmium 50 mM 12 hrs, larvae L3  (562) Cadmium 50 mM 48 hrs, 4-day adult  (229) Cadmium 100 mM 48 hrs, 4-day adult  (331) Copper 0.5 mM 12 hrs, larvae L3    57 Copper 15 mM 48 hrs, 4-day adult  (911) Zinc 5 mM 12 hrs, larvae L3    67 Zinc 4.5 mM 48 hrs, 4-day adult  (624) Ethanol 2.5% 3 hrs, larvae L3  (167) Ethanol 5% 3 hrs, larvae L3  (439) Ethanol 10% 3 hrs, larvae L3  (155) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (549) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (353) Caffeine 25 mg/ml 48 hrs, 4-day adult  (148) Paraquat 5 mM 48 hrs, 4-day adult  (245) Paraquat 10 mM 48 hrs, 4-day adult  (245) Rotenone 2 μg 12 hrs, larvae L3  (226) Rotenone 8 μg 12 hrs, larvae L3  136 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    102 cold shock, 4-day adult    77 heat shock, 4-day adult    163 Cadmium 50 mM 6 hrs, larvae L3    159 Cadmium 50 mM 12 hrs, larvae L3    562 Cadmium 50 mM 48 hrs, 4-day adult    229 Cadmium 100 mM 48 hrs, 4-day adult    331 Copper 0.5 mM 12 hrs, larvae L3    57 Copper 15 mM 48 hrs, 4-day adult    911 Zinc 5 mM 12 hrs, larvae L3    67 Zinc 4.5 mM 48 hrs, 4-day adult    624 Ethanol 2.5% 3 hrs, larvae L3    167 Ethanol 5% 3 hrs, larvae L3    439 Ethanol 10% 3 hrs, larvae L3    155 Caffeine 1.5 mg/ml 4 hrs, larvae L3    549 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    353 Caffeine 25 mg/ml 48 hrs, 4-day adult    148 Paraquat 5 mM 48 hrs, 4-day adult    245 Paraquat 10 mM 48 hrs, 4-day adult    245 Rotenone 2 μg 12 hrs, larvae L3    226 Rotenone 8 μg 12 hrs, larvae L3    136 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_25 mE1_20_mRNA_expression_cluster_10
External Data & Images
Linkouts	BDGP expression data • LD05816 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0052672 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG32672-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable Atg8aKG07569 viable, with Scer\GAL4pnr-MD237 Atg8aGD4654 Other Phenotypes Allele short lived | male, with Scer\GAL4[-]: Atg8aEP362 Phenotype manifest in Allele embryonic/larval fat body | somatic clone | third instar larval stage, with Scer\GAL4Act5C.PP Atg8aKK102155 embryonic/larval salivary gland Atg8aKG07569
Classical Alleles ( 7 )
For All Classical Alleles Show
Allele of Atg8a Class Mutagen Stocks Known lesion Atg8aEP362 P-element activity 1 -- Atg8aKG07569 P-element activity 1 Yes Atg8a2 0 -- Atg8ad4 P-element activity 0 Yes Atg8aex3 P-element activity 0 Yes Atg8aG9749 P-element activity 0 -- Atg8aGG01244 0 --
Alleles Carried on Transgenic Constructs ( 13 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Atg8a Class Mutagen Stocks Known lesion Atg8aGD4654 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Atg8aHMS01328 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Atg8aJF02895 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Atg8aScer\UAS.P\T.T:Disc\RFP-mCherry in vitro construct - regulatory fusion in vitro construct - coding region fusion 1 Yes Atg8aScer\UAS.T:Avic\GFP-EGFP,T:Disc\RFP-mCherry in vitro construct - regulatory fusion in vitro construct - coding region fusion 1 Yes Atg8ahs.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 -- Atg8aKK102155 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Atg8aScer\UAS.P\T.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Atg8aScer\UAS.P\T.T:Avic\GFP in vitro construct - coding region fusion in vitro construct - regulatory fusion 0 Yes Atg8aScer\UAS.T:Avic\GFP in vitro construct - regulatory fusion 0 Yes Atg8aScer\UAS.T:Disc\RFP-mCherry in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Atg8aScer\UAS.T:Disc\RFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Atg8aT:Avic\GFP in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Partially disrupted in	Df(1)Atg8ad4 (Pircs et al., 2012)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD4654} NA P{KK102155} NA P{TRiP.HMS01328} NA P{TRiP.JF02895} NA P{UAS-Atg8a.GFP} NA P{UAS-Atg8a.RFP} NA P{UAS-mCherry-Atg8a} NA P{UASp-Atg8a.EGFP} NA P{UASp-GFP-Atg8a} NA P{UASp-GFP-mCherry-Atg8a} NA P{UASp-mCherry-Atg8a} NA heat-shock construct P{hs-Atg8a.GFP} NA characterization construct P{GFP-Atg8a.D} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}Atg8aEP362 NA P{EP}Atg8aG9749 NA P{Mae-UAS.6.11}Atg8aGG01244 NA miscellaneous insertions P{SUPor-P}Atg8aKG07569 NA insertion of enhancer trap P{lacW}l(1)G0076G0076 No
Gene Ontology: Function, Process & Cellular Component ( 6 unique terms )
Terms Based on Experimental Evidence ( 6 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
determination of adult lifespan inferred from mutant phenotype (Simonsen et al., 2007) macroautophagy inferred from expression pattern (Scott et al., 2004) regulation of autophagy inferred from mutant phenotype (Pircs et al., 2012) regulation of protein ubiquitination inferred from mutant phenotype (Simonsen et al., 2007)
Cellular Component
CV term Evidence References
amphisome inferred from direct assay (Wong et al., 2012) autophagic vacuole inferred from direct assay (Scott et al., 2004, Wong et al., 2012)
Terms Based on Predictions or Assertions ( 0 terms )
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Atg8a - Atg7 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Atg8a - CG11474 experimental knowledge based (Guruharsha et al., 2011) Atg8a - Pvf1 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Atg8a - Su(var)205 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Atg8a allele Gene References unspecified bchs (Simonsen et al., 2007)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 58422 DroID - A comprehensive database of gene and protein interactions. • FBgn0052672 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 32001
Orthologs
OrthoDB Orthologs (47) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6MPJ9T Dipteran inclusive ortholog search EOG61NTWG Insect inclusive ortholog search EOG6HDRR8 Arthropod inclusive ortholog search EOG60001Z Metazoa inclusive ortholog search EOG6X9MCM
Orthologs in Drosophila Species (EOG6MPJ9T)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Atg8a     Drosophila simulans   Dsim\GD16015 Y   Drosophila sechellia   Dsec\GM11288 Y   Drosophila erecta   Dere\GG18902 Y   Drosophila yakuba   Dyak\GE15373 Y   Drosophila ananassae   Dana\GF20824 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA22662 Y   Drosophila persimilis   Dper\GL26902 Y   Drosophila willistoni   Dwil\GK16501 Y   Drosophila virilis   Dvir\GJ14868 Y   Drosophila mojavensis   Dmoj\GI15162 Y   Drosophila grimshawi   Dgri\GH12106 Y
Orthologs in non-Drosophila Dipterans (EOG61NTWG)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL007162   Anopheles gambiae Malaria mosquito  Agam\AGAP002685   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ002897
Orthologs in non-Dipteran Insects (EOG6HDRR8)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\Atg8a   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG012601   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH21110   Atta cephalotes Leafcutter ant  Acep\ACEP22270   Camponotus floridanus Florida carpenter ant  Cflo\CFLO12865   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL17777   Linepithema humile Argentine ant  Lhum\LH16884   Acyrthosiphon pisum Pea aphid  Apim\ACYPI004701   Bombyx mori Silkmoth  Bmor\BGIBMGA011783   Pediculus humanus Human body louse  Phum\PHUM129160   Tribolium castaneum Red flour beetle  Tcas\GLEAN_15729-OG23899
Orthologs in non-Insect Arthropods (EOG60001Z)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Ixodes scapularis Deer tick  Isca\ISCW017654
Orthologs in non-Arthropod Metazoa (EOG6X9MCM)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\lgg-1   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_002177gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_006271gn   Danio rerio Zebrafish  Drer\gabarapl2   Danio rerio Zebrafish  Drer\gabarap   Danio rerio Zebrafish  Drer\ENSDARG00000052082   Xenopus tropicalis Western clawed frog  Xtro\gabarapl1   Xenopus tropicalis Western clawed frog  Xtro\gabarapl2   Gallus gallus Domestic chicken  Ggal\GABARAPL3   Gallus gallus Domestic chicken  Ggal\GABARAPL2   Mus musculus House mouse  Mmus\Gabarapl1   Mus musculus House mouse  Mmus\Gabarapl2   Rattus norvegicus Norway rat  Rnor\RGD1562165   Rattus norvegicus Norway rat  Rnor\Gabarapl2   Rattus norvegicus Norway rat  Rnor\ENSRNOG00000039870   Rattus norvegicus Norway rat  Rnor\Gabarap   Rattus norvegicus Norway rat  Rnor\Gabarapl1   Homo sapiens Human  Hsap\GABARAPL2   Homo sapiens Human  Hsap\GABARAPL3   Homo sapiens Human  Hsap\GABARAP
Human Orthologs (3)
Gene OMIM HGNC Hsap\GABARAPL2     Hsap\GABARAPL3     Hsap\GABARAP
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD16015 Drosophila sechellia Dsec\GM11288 Drosophila erecta Dere\GG18902 Drosophila yakuba Dyak\GE15373 Drosophila ananassae Dana\GF20824 Drosophila pseudoobscura pseudoobscura Dpse\GA22662 Drosophila persimilis Dper\GL26902 Drosophila willistoni Dwil\GK16501 Drosophila virilis Dvir\GJ14868 Drosophila mojavensis Dmoj\GI15162 Drosophila grimshawi Dgri\GH12106
Stocks & Reagents
Stocks Listed in FlyBase ( 8 )
Bloomington	10107 w1118 P{EP}Atg8aEP362 14639 y1 P{SUPor-P}Atg8aKG07569/FM7c 37749 y1 w1118; P{UASp-GFP-mCherry-Atg8a}2 34340 y1 sc* v1; P{TRiP.HMS01328}attP2 28989 y1 v1; P{TRiP.JF02895}attP2 e*/TM3, Sb1 37750 y1 w1118; P{UASp-mCherry-Atg8a}2; Dr1/TM3, Ser1
VDRC	v43096 w1118; P{GD4654}v43096 v43097 w1118; P{GD4654}v43097
Genomic Clones ( 1 )
	CH221-17A11 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 250 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD05816
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH13867 GM05049 HL05503 LP11903 RE09885 RE14084 RE15983 RE16276 RE19134 RE19146 RE22355 RE25657 RE25669 RE27567 RE32012 RE33895 RE37417 RE42929 RE49975 RE55148 RE55626 RE64216 RE65869 RE74286 RH02802 RH51178 RH58526 RH58851 RH66338 RH70759 RH71446 RH72350 SD22207 SD24081 SD24723
Other clones	10787 14886 15127 16561 34675 47075 53716 56499 60386 61904 62623 64727 69550 69640 74874 76571 99064 105025 106000 107419 120304 144303 144676 144723 161639 167122.1 167380.1 179227 183417 194227 211206.1 214213.13 241116 273991 274593 277223 277225 524744 527101 557774 575648 bs19a08 bs31c04 bs69b09 CK01.7C.G10 CK01.8B.D7 CK01.22B.H12 CK01.24A.F7 CK01.53B.E12 CK01.53B.F12 CK01.60C.A12 CK01.66D.F2 CK01.77B.H3 CK01.87C.E10 CK01.113A.D2 CK01.117A.D3 CK01.125A.H6 CK01.132C.C6 Dmel_dig_016094_0631_3148 Dmel_dig_016502_0691_3234 Dmel_neb_011861_0583_1677 Dmel_neb_013383_0192_3166 EC23401 EC25652 EC26675 EC27604 EC30736 EC36736 EK005411 EK010644 EK011210 EK012802 EK039250 EK042952 EK042956 EK047601 EK048736 EK048905 EK054053 EK063536 EK069249 EK095461 EK101043 EK110842 EK118332 EK119548 EK169134 EK171259 EK177401 EK178114 EK184643 EK185337 EK188528 EK192956 EK195842 EK198817 EK205043 EK207303 EK213004 EK220805 EK233628 EK250119 EK250719 EK251655 EK256102 EK272251 EK288455 EK291115 EK301110 EK302422 EK304126 EK304130 EN00348 EN05839 EN07255 EN12352 EN17464 EP10715 ESG011a.E7.4_D04 ESG012b.TE_A06 ESG019c.E21_F01 ESG0116a.B21_A11 FGA006A11 FGA007H03 FGA008E07 FGC016H07 FGC031B10 FGC033C02 FGH104E03 FGH106E10 FGH108G06 FGH110G06 FGH112F09 FGH114G04 FGH117A11 FGH117C03 FGH120G01 FGH124E10 FGH127C09 FGM001A02 FGM003H01 FGM004H09 FGM209G11 FGM230C01 FGM235D10 FGM301G03 FGM303G06 FGM315A10 FGM317E02 FGM322B11 FGM324G02 FGX001G08 IP10221 RH01033 RH01291 RP003007061 RP003019675 RP003021042 RP003046906 RP003050071 RP003051558 RP003052743 RP003063793 RP003078766 RP003083882 RP003086119 RP003089759 RP003090708 RP003100031 RP003138499 RP003138576 RP003154941 RP003162336 RP003187636 RP003223512 RP003234732 RP003244336 RP003245235 RP003276285 RP003301502 RP003325221 RP003335190 RP003342709 RP003349404 RP003351822 RP003353287 RP003356751 RP003371313 RP003371961 RP003372024 RP003379449 RP003379590 RP003385205 RP003399948 RP003409492 RP003410169 RP003422593 RP003446429 RP003447005 RP003461483 RP003489942 RP003496993 RP003526807 RP003536298 RP003570749 RP003582638 RP003600818 RP003613020 RP003619253 RP003621312 RP003642508 RP003643468 RP003645203 RP003646300
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0052672 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 32001
Antibody Information
	polyclonal (Barth et al., 2011)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Atg8a CG32672 (Scott et al., 2004) Source for identity of: Atg8a CG32672 (Scott, 2005.3.2) Source for identity of: Atg8a CG32672 (Scott et al., 2004)
Source for database merge of	Source for merge of: CG32672 BcDNA:LD05816 (FlyBase, 1996-)
Additional comments	The Atg8b gene may have been derived from the Atg8a gene by retroposition. (Dai et al., 2006) Source for merge of CG32672 BcDNA:LD05816 was a shared cDNA (date:030728). (FlyBase Genome Annotators, 2002-2003, FlyBase, 1996-) "l(1)G0076" may affect "CG32672". (Peter et al., 2002)
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_167245 RefSeq (Proteins) • NP_727447 DDBJ / EMBL / GenBank • AA246827 AAF46617 AAM52664 AI297572 AI945976 AI946825 AQ025324 AW941604 AY122152 BE662180 Entrez Gene - A searchable database of RefSeq genes. • 32001 UniProtKB/TrEMBL • Q9W2S2
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Autophagy protein Atg8 ubiquitin-like (IPR004241) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD05816
Linkouts
	BioGRID - A database of protein and genetic interactions • 58422 DroID - A comprehensive database of gene and protein interactions. • FBgn0052672 DRSC - Results from RNAi screens. • FBgn0052672 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0052672 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG32672-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0052672 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 32001 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 32001 modMine - Data generated by the modENCODE project. • FBgn0052672
Synonyms & Secondary IDs ( 16 )
Reported As
Symbol Synonym	atg8 (Berry and Baehrecke, 2007, Wang et al., 2012) Atg8 (Haberman et al., 2010, Midorikawa et al., 2010, Köhler et al., 2009, Liu et al., 2011, Demontis and Perrimon, 2010, Lee et al., 2011, Rahman et al., 2012, Williamson et al., 2010) ATG8 (Füger et al., 2012, Wong et al., 2012) Atg8/LC3 (Juhász et al., 2008) ATG8a (Scott, 2005.3.2) Atg8a (Goodliffe et al., 2007, Botella et al., 2007, Langille and Clark, 2007, Nezis et al., 2009, Kadandale et al., 2010, Simonsen et al., 2007, Zeitouni et al., 2007, Juhász et al., 2007, Juhász et al., 2008, Hou et al., 2008, Scott et al., 2007, Ling et al., 2009, Kim et al., 2008, Csikós et al., 2009, Shelly et al., 2009, Scott et al., 2007, Juhász et al., 2007, Chang and Neufeld, 2009, Rusten et al., 2007, Arsham and Neufeld, 2009, Denton et al., 2009, McPhee et al., 2010, Nezis et al., 2010, Bass et al., 2009, Li et al., 2010, Barth et al., 2011, Köhler et al., 2009, Nisoli et al., 2010, Francis et al., 2010, Simonsen et al., 2008, Tang et al., 2011, Érdi et al., 2012, Kim et al., 2010, Pircs et al., 2012) atg8a (Simonsen et al., 2007, Sakurai et al., 2010, Nezis et al., 2008, Midorikawa et al., 2010, Haberman et al., 2012) Atg8A (Liu et al., 2009) BcDNA:LD05816   CG1534 (Peter et al., 2002, Betran et al., 2002, Zinke et al., 2002, ) CG32672 (FlyBase, 1996-, Dai et al., 2006, Nezis et al., 2009, Bai et al., 2008, Juhász et al., 2007, Rusten et al., 2007, Perkins et al., 2009, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Érdi et al., 2012) DrAtg8a (Lindmo et al., 2008) LC3 (Mohseni et al., 2009, Tang et al., 2011, Pimenta de Castro et al., 2012) LC3/Atg8 (Haberman et al., 2012) unnamed (Scott and Neufeld, 2003)
Name Synonym	Autophagy-specific gene 8a (Scott et al., 2004, Scott, 2005.3.2, Scott et al., 2004)
Secondary FlyBase IDs
	FBgn0030229 FBgn0063168
References ( 87 )
Generate a list of	All references relating to this gene ( 87 )
List References by type	Research paper  ( 62 ) Supplementary material  ( 9 ) Personal communication to FlyBase  ( 9 ) Abstract  ( 2 ) FlyBase analysis  ( 4 ) DNA/RNA sequence record  ( 1 )
Recent research papers ( 17 )
	Burman et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109(26): 10438--10443 Analysis of neural subtypes reveals selective mitochondrial dysfunction in dopaminergic neurons from parkin mutants. [FBrf0218718] Díaz-Castillo and Ranz, 2012, Mol. Biol. Evol. 29(9): 2105--2108 Nuclear chromosome dynamics in the Drosophila male germ line contribute to the nonrandom genomic distribution of retrogenes. [FBrf0219274] Füger et al., 2012, PLoS Genet. 8(11): e1003066 Spastic Paraplegia Mutation N256S in the Neuronal Microtubule Motor KIF5A Disrupts Axonal Transport in a Drosophila HSP Model. [FBrf0220146] Haberman et al., 2012, J. Cell Biol. 196(2): 261--276 The synaptic vesicle SNARE neuronal Synaptobrevin promotes endolysosomal degradation and prevents neurodegeneration. [FBrf0217255] Pimenta de Castro et al., 2012, Cell Death Differ. 19(8): 1308--1316 Genetic analysis of mitochondrial protein misfolding in Drosophila melanogaster. [FBrf0218837] Pircs et al., 2012, PLoS ONE 7(8): e44214 Advantages and Limitations of Different p62-Based Assays for Estimating Autophagic Activity in Drosophila. [FBrf0219364] Poels et al., 2012, Cell Death Differ. 19(10): 1590--1601 Autophagy and phagocytosis-like cell cannibalism exert opposing effects on cellular survival during metabolic stress. [FBrf0219353] Rahman et al., 2012, Traffic 13(12): 1680--1692 Drosophila mauve Mutants Reveal a Role of LYST Homologs Late in the Maturation of Phagosomes and Autophagosomes. [FBrf0220730] Wang et al., 2012, Mol. Cell. Biol. 32(12): 2203--2213 LST8 Regulates Cell Growth via Target-of-Rapamycin Complex 2 (TORC2). [FBrf0218417] Wong et al., 2012, Curr. Biol. 22(17): 1616--1621 Drosophila TRPML Is Required for TORC1 Activation. [FBrf0219457] Érdi et al., 2012, Autophagy 8(7): 1124--1135 Loss of the starvation-induced gene Rack1 leads to glycogen deficiency and impaired autophagic responses in Drosophila. [FBrf0219844] Barth et al., 2011, Cell Death Differ. 18(6): 915--924 Autophagy in Drosophila ovaries is induced by starvation and is required for oogenesis. [FBrf0213616] Lee et al., 2011, Dev. Biol. 356(2): 588--597 UVRAG is required for organ rotation by regulating Notch endocytosis in Drosophila. [FBrf0214470] Liu et al., 2011, J. Neurosci. 31(6): 2052--2063 Drosophila Acyl-CoA Synthetase Long-Chain Family Member 4 Regulates Axonal Transport of Synaptic Vesicles and Is Required for Synaptic Development and Transmission. [FBrf0212968] Moskalev et al., 2011, Biogerontology 12(3): 253--263 Radiation hormesis and radioadaptive response in Drosophila melanogaster flies with different genetic backgrounds: the role of cellular stress-resistance mechanisms. [FBrf0213740] Tang et al., 2011, EMBO J. 30(4): 636--651 Atg1-mediated myosin II activation regulates autophagosome formation during starvation-induced autophagy. [FBrf0213047] Tanner et al., 2011, Development 138(2): 327--338 Bcl-2 proteins and autophagy regulate mitochondrial dynamics during programmed cell death in the Drosophila ovary. [FBrf0212552] Show all research papers ...