FlyBase Gene Report: Dmel\Muc4B

General Information

Symbol

Dmel\Muc4B

Species

D. melanogaster

Name

Mucin 4B

Annotation Symbol

CG32774

Feature Type

protein_coding_gene

FlyBase ID

FBgn0052774

Gene Model Status

Current

Stock Availability

5 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

4B4-4B4

Sequence location

X:4,244,095..4,246,139 [-]

Recombination map

1-7

RefSeq locus

NC_004354 REGION:4244095..4246139

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (4 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables extracellular matrix structural constituent

inferred from sequence model

(Syed et al., 2008)

Biological Process (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in egg chorion assembly

inferred from expression pattern

(Tootle et al., 2011)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in egg chorion assembly

inferred from genomic context

(Tootle et al., 2011)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

located_in egg chorion

inferred from genomic context

(Tootle et al., 2011)

located_in extracellular matrix

inferred from sequence model

(Syed et al., 2008)

Gene Group (FlyBase)

MUCINS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

MUCINS -: Mucin/mucin-like proteins are heavily O-glycosylated proteins characterized by proline, threonine and serine tandem repeats. Mucins form extracellular matrix networks or act as mucosal secretion lubricants. (Adapted from FBrf0205867.)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Muc4B for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9W4M2)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.52

Gene model reviewed during 5.56

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Muc4B-RB

FBtr0307050

NM_167006

1716

496

Muc4B-RC

FBtr0343573

NM_001297927

1987

496

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Muc4B-PB

FBpp0297893

51.2

496

4.87

FBpp0310173

51.2

496

4.87

Q9W4M2

NP_001284856

AHN59327

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

496 aa isoforms: Muc4B-PB, Muc4B-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9W4M2

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Muc4B using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.08

Transcript Expression

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism

(Syed et al., 2008)

third instar larval stage

embryonic/larval salivary gland

(Syed et al., 2008)

organism

(Syed et al., 2008)

pupal stage

organism

(Syed et al., 2008)

adult stage | female

organism

(Syed et al., 2008)

adult stage | male

organism

(Syed et al., 2008)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Muc4B in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Muc4B

View exonic expression by tissue for Dmel\Muc4B

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

31368/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0052774

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0052774

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0052774

Flygut - An atlas of the Drosophila adult midgut

FBgn0052774

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 0 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Muc4B

Transgenic constructs containing regulatory region of Muc4B

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 13 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^pnr-MD237

Muc4B^GD13127

partially lethal - majority die | pupal stage, with Scer\GAL4^pnr-MD237

Muc4B^GD13127

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Muc4B^GD13127

viable, with Scer\GAL4^Mef2.PR

Muc4B^GD13127

Other Phenotypes

Allele

visible, with Scer\GAL4^pnr-MD237

Muc4B^GD13127

Phenotype manifest in

Allele

trichogen cell, with Scer\GAL4^pnr-MD237

Muc4B^GD13127

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (0)

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (0)

Mus musculus (laboratory mouse) (0)

Xenopus tropicalis (Western clawed frog) (0)

Danio rerio (Zebrafish) (0)

Caenorhabditis elegans (Nematode, roundworm) (2)

Cele\clec-174

1 of 14

Yes

Cele\cpg-19

1 of 14

Yes

Anopheles gambiae (African malaria mosquito) (1)

Agam\AgaP_AGAP010704

1 of 12

Yes

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Muc4B. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (3)

Muc55B

2 of 13

CG7567

1 of 13

CG11470

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Other Interaction Browsers

Summary of Genetic Interactions

esyN Network Diagram

Other Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0052774

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-7

Cytogenetic map

4B4-4B4

Sequence location

X:4,244,095..4,246,139 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

4B4-4B4

Limits computationally determined from genome sequence between P{EP}CG2930^EP1352 and P{EP}EP425

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

1-7

(FlyBase, 2016)

1-6.5

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (5)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

67940

y¹ v¹; P{TRiP.HMS05774}attP2

76072

y¹ v¹; P{TRiP.HMS05885}attP2/TM3, Sb¹

NIG-Fly

HMJ23062

y¹ v¹; P{TRiP.HMJ23062}attP40/CyO

VDRC

v42100

w¹¹¹⁸; P{GD13127}v42100

v42101

w¹¹¹⁸; P{GD13127}v42101/CyO

Genomic Clones (20)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (6)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0052774

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Source for merge of: CG15574 CG15575

(FlyBase Genome Annotators, 2002-2003)

Additional comments

Annotations CG15574 and CG15575 merged as CG32774 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Nomenclature History

Source for database identify of

Source for identity of: Muc4B CG32774

(Syed et al., 2008)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

CG15574

CG15575

(Wang et al., 2004)

CG32774

(National Institute of Genetics Fly Stocks, 2023-, Hosono et al., 2015, Tootle et al., 2011, Perkins et al., 2009, Syed et al., 2008, Yakoby et al., 2008)

Muc4B

(Isaacson et al., 2024, Miao et al., 2024, Pignataro et al., 2024, Bossen et al., 2023, Pang et al., 2021, Lefebvre et al., 2016, Hsu et al., 2015, Syed et al., 2008)

muc4B

(Tootle et al., 2011)

Name Synonyms

CG32774

Muc4B

Mucin 4B

(Syed et al., 2008)

Mucin4B

(Li et al., 2022)

Secondary FlyBase IDs

FBgn0029699
FBgn0040903

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 23 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

31368

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9W4M2

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q9W4M2

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9W4M2

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

31368/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0052774

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0052774

FlyMine - An integrated database for Drosophila genomics

FBgn0052774

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG32774

MARRVEL_MODEL - MARRVEL (model organism gene)

31368

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0052774

DRSC - Results frm RNAi screens

FBgn0052774

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0052774

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0052774

Flygut - An atlas of the Drosophila adult midgut

FBgn0052774

References (26)

The following external sites may use different assemblies or annotations than FlyBase.

0.08

Report Sections