FlyBase Gene Report: Dmel\CG33129

General Information

Symbol

Dmel\CG33129

Species

D. melanogaster

Name

Annotation Symbol

CG33129

Feature Type

protein_coding_gene

FlyBase ID

FBgn0053129

Gene Model Status

Stock Availability

9 publicly available

Gene Snapshot

In progress.Contributions welcome.

Other Summaries

Alliance
Auto summary
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

Sequence location

2L:10,964,905..10,970,275 [-]

Recombination map

2-44

RefSeq locus

NT_033779 REGION:10964905..10970275

Sequence

Get Decorated FASTA

Genomic Maps

GBrowse JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

[Detailed GO annotations]

Protein Family (UniProt)

-

Protein Signatures (InterPro)

Molecular Function (GO)
[Detailed GO annotations]

Experimental Evidence

-

Predictions / Assertions

-

Summaries

Gene Model and Products

Number of Transcripts

7

Number of Unique Polypeptides

2

Please see the GBrowse view of Dmel\CG33129 or the JBrowse view of Dmel\CG33129 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.51

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.55

Sequence Ontology: Class of Gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0080170

1504

237

FBtr0080171

1533

237

FBtr0080172

1473

237

FBtr0080169

2652

677

FBtr0340550

2815

677

FBtr0340551

1782

237

FBtr0340552

1661

237

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

FBpp0079759

25.8

237

10.33

FBpp0079760

25.8

237

10.33

FBpp0079761

25.8

237

10.33

FBpp0079758

75.7

677

9.92

FBpp0309448

75.7

677

9.92

FBpp0309449

25.8

237

10.33

FBpp0309450

25.8

237

10.33

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

677 aa isoforms: CG33129-PE, CG33129-PF

237 aa isoforms: CG33129-PA, CG33129-PB, CG33129-PC, CG33129-PG, CG33129-PH

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CG33129 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Gene Ontology (4 terms)

Molecular Function (0 terms)

There is no information supporting a Molecular Function term for this gene. (Based on a review of available data on: 2003-04-10 )

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

response to hyperoxia

inferred from mutant phenotype

(Zhao et al., 2010)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

endomembrane system

inferred from high throughput direct assay

(Tan et al., 2009)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

endoplasmic reticulum

inferred from biological aspect of ancestor with PANTHER:PTN000335433

(assigned by GO_Central )

(Gaudet et al., 2011)

Golgi apparatus

inferred from biological aspect of ancestor with PANTHER:PTN000335433

(assigned by GO_Central )

(Gaudet et al., 2011)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

dorsal epidermis primordium

Comment: reported as dorsal epidermis anlage

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

ventral ectoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

dorsal epidermis primordium

(Fisher et al., 2012)

dorsal head epidermis primordium

Comment: reported as head epidermis primordium

(Fisher et al., 2012)

lateral head epidermis primordium

Comment: reported as head epidermis primordium

(Fisher et al., 2012)

ventral head epidermis primordium

Comment: reported as head epidermis primordium

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

ventral epidermis primordium

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 7 of adulthood -- day 49 of adulthood

(Cammarato et al., 2011)

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

endomembrane system

inferred from high throughput direct assay

(Tan et al., 2009)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\CG33129 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\CG33129

View exonic expression by tissue for Dmel\CG33129

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0053129

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0053129

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG33129-RC

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG33129

Flygut - An atlas of the Drosophila adult midgut

FBgn0053129

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\CG33129

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 9 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 6 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of CG33129

Transgenic constructs containing regulatory region of CG33129

Deletions and Duplications ( 1 )

Disrupted in

(Ryder, 2004.4.26)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

partially lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

CG33129^GD1081

CG33129^f07745

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

CG33129^GD11811

viable, with Scer\GAL4^Mef2.PR

CG33129^GD11811

viable, with Scer\GAL4^pnr-MD237

viable, with Scer\GAL4^tin.CΔ4

CG33129^GD11811

Sterility

Allele

CG33129^f07745

Other Phenotypes

Allele

long lived | conditional

CG33129^f07745

oxidative stress response defective

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

13 of 15

Yes

Yes

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

13 of 15

Yes

Yes

Rattus norvegicus (Norway rat) (1)

13 of 13

Yes

Yes

Xenopus tropicalis (Western clawed frog) (1)

1 of 12

Yes

Yes

Danio rerio (Zebrafish) (1)

12 of 15

Yes

Yes

Caenorhabditis elegans (Nematode, roundworm) (0)

No records found.

Arabidopsis thaliana (thale-cress) (4)

3 of 9

Yes

Yes

2 of 9

No

Yes

2 of 9

No

Yes

1 of 9

No

Yes

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091904IW )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila suzukii

Spotted wing Drosophila

Dsuz\DS10_00001872

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Drosophila persimilis

Drosophila willistoni

Drosophila virilis

Drosophila mojavensis

Drosophila grimshawi

Drosophila grimshawi

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091502N2 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Glossina morsitans

Tsetse fly

Gmor\GMOY002629

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_03942

Mayetiola destructor

Hessian fly

Mdes\Mdes011326

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL000944

Anopheles darlingi

American malaria mosquito

Adar\ADAC009780

Anopheles gambiae

Malaria mosquito

Agam\AGAP009823

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ000883

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0DIO )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA001842

Danaus plexippus

Monarch butterfly

Dple\DPOGS209131-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL008143

Apis florea

Little honeybee

Aflo\2907755108

Apis mellifera

Western honey bee

Bombus impatiens

Common eastern bumble bee

Bombus terrestris

Buff-tailed bumblebee

Linepithema humile

Argentine ant

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:000487

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG011515

Dendroctonus ponderosae

Mountain pine beetle

Tribolium castaneum

Red flour beetle

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_03706

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X08PC )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR000551

Ixodes scapularis

Black-legged tick

Isca\ISCW020051

Stegodyphus mimosarum

African social velvet spider

Smim\KFM72027.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur04g00040

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_242020

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0WVG )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Tmem214_1

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Tmem214

Ciona intestinalis

Vase tunicate

Cint\LOC100186389

Gallus gallus

Domestic chicken

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (0)

No records found.

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

TMEM214; transmembrane protein 214

13 of 15

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

RNA-protein

Physical Interaction

Assay

References

anti tag coimmunoprecipitation, quantitative reverse transcription pcr, full identification by DNA sequencing

(Vazquez-Pianzola et al., 2017)

protein-protein

Physical Interaction

Assay

References

CG33129 - CG2064

experimental knowledge based

(Guruharsha et al., 2011)

CG33129 - Ugt36D1

experimental knowledge based

(Guruharsha et al., 2011)

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

60584

DroID - A comprehensive database of gene and protein interactions.

FBgn0053129

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

34514

MIST (protein-protein) - An integrated Molecular Interaction Database

CG33129

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

2L

Recombination map

2-44

Cytogenetic map

Sequence location

2L:10,964,905..10,970,275 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

32C1-32C1

Limits computationally determined from genome sequence between P{PZ}dbf¹ and P{lacW}SCAR^k03107

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-44

(FlyBase, 2016)

2-37.1

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (9)

12754

w¹¹¹⁸; P{GT1}CG33129^BG02672

19104

w¹¹¹⁸; PBac{WH}CG33129^f07745

85268

w¹¹¹⁸; PBac{WH}CG33129^f07748

15213

y¹; P{SUPor-P}CG33129^KG09776; ry⁵⁰⁶

33751

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01091}attP2

104232

w^*; P{GawB}CG33129^NP2532 / CyO

v107365

P{KK106525}VIE-260B

v9699

w¹¹¹⁸ P{GD1081}v9699

v27554

w¹¹¹⁸; P{GD11811}v27554

Genomic Clones (16)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (299)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

Other clones

DPiM_AT01533

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0053129

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0053129

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

34514

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: CG6087 CG6089

(FlyBase Genome Annotators, 2002-2003)

Additional comments

Annotations CG6087 and CG6089 merged as CG33129 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Other Comments

Host gene for maternally inherited stable intronic sequence RNA (sisRNA).

(Tay and Pek, 2017)

Candidate stable intronic sequence RNA (sisRNA) identified within 5'UTR of this gene.

(Pek et al., 2015)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 60 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34514

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0053129

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0053129

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG33129

Flygut - An atlas of the Drosophila adult midgut

FBgn0053129

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

34514

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC009435

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG33129

modMine - A data warehouse for the modENCODE project

FBgn0053129

Linkouts

BioGRID - A database of protein and genetic interactions.

60584

DPiM - Drosophila Protein interaction map

FBgn0053129

DroID - A comprehensive database of gene and protein interactions.

FBgn0053129

DRSC - Results frm RNAi screens

FBgn0053129

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0053129

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG33129-RC

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0053129

FlyMine - An integrated database for Drosophila genomics

FBgn0053129

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

34514

MIST (protein-protein) - An integrated Molecular Interaction Database

CG33129

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Name Synonyms

Secondary FlyBase IDs

FBgn0032319
FBgn0032320

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (57)

Report Sections