FlyBase Gene Report: Dmel\Nadk1b

General Information

Symbol

Dmel\Nadk1b

Species

D. melanogaster

Name

NAD kinase 1b

Annotation Symbol

CG33156

Feature Type

protein_coding_gene

FlyBase ID

FBgn0053156

Gene Model Status

Current

Stock Availability

10 publicly available

Enzyme Name (EC)

NAD(+) kinase (2.7.1.23)

Gene Summary

NAD kinase 1b (Nadk1b) encodes a putative enzyme predicted to phosphorylate NAD(+) to form NADP(+). [Date last reviewed: 2025-01-23] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

50B1-50B1

Sequence location

2R:13,548,244..13,553,240 [-]

Recombination map

2-68

RefSeq locus

NT_033778 REGION:13548244..13553240

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (5 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables NAD+ kinase activity

inferred from sequence or structural similarity with SGD:S000003810

(Gene Ontology Curators, 2002-)

inferred from electronic annotation with InterPro:IPR002504, InterPro:IPR017437

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000463643

(GO Reference Genome Project, 2011-)

Biological Process (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in NAD metabolic process

inferred from electronic annotation with InterPro:IPR017437

(InterPro Project Members, 2004-)

involved_in NADP biosynthetic process

inferred from biological aspect of ancestor with PANTHER:PTN000463643

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR002504

(InterPro Project Members, 2004-)

inferred from sequence or structural similarity with SGD:S000003810

(Gene Ontology Curators, 2002-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from sequence or structural similarity with SGD:S000003810

(Gene Ontology Curators, 2002-)

located_in nucleus

inferred from sequence or structural similarity with SGD:S000003810

(Gene Ontology Curators, 2002-)

Gene Group (FlyBase)

UNCLASSIFIED KINASES

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

NAD+ kinase activity

NAD(+) + ATP = ADP + NADP(+) + H(+) (2.7.1.23)

RHEA 18629:

Summaries

Gene Snapshot

NAD kinase 1b (Nadk1b) encodes a putative enzyme predicted to phosphorylate NAD(+) to form NADP(+). [Date last reviewed: 2025-01-23]

Gene Group (FlyBase)

UNCLASSIFIED KINASES -: This group comprises kinases that do not classify under other groups in FlyBase.

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Nadk1b for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry A1Z9F4)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.50

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Nadk1b-RA

FBtr0087682

NM_176165

1874

454

Nadk1b-RB

FBtr0087684

NM_176167

1824

453

Nadk1b-RC

FBtr0087681

NM_176168

2030

375

Nadk1b-RE

FBtr0087683

NM_176166

2330

490

Nadk1b-RF

FBtr0113450

NM_001103832

1795

450

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Nadk1b-PA

FBpp0086802

50.0

454

4.97

FBpp0086804

50.7

453

5.83

FBpp0086801

41.7

375

5.11

FBpp0086803

54.8

490

7.14

FBpp0112362

49.9

450

5.50

A8DYC9

NP_001097302

ABV53790

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Nadk1b using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.02

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

ring gland

(Nakaoka et al., 2017)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

ring gland

(Nakaoka et al., 2017)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Nadk1b in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Nadk1b

View exonic expression by tissue for Dmel\Nadk1b

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0053156

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36507/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0053156

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0053156

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0053156

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG33156

Flygut - An atlas of the Drosophila adult midgut

FBgn0053156

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 4 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 7 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Nadk1b

Transgenic constructs containing regulatory region of Nadk1b

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 3 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal - all die before end of larval stage, with Scer\GAL4^2-286

Nadk1b^NIG.6152R

viable

Nadk1b^MB05931

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

viable, with Scer\GAL4^Mef2.PR

viable, with Scer\GAL4^pnr-MD237

viable, with Scer\GAL4^tin.CΔ4

Nadk1b^GD15181

Other Phenotypes

Allele

abnormal developmental rate, with Scer\GAL4^phtm.PU

Nadk1b^NIG.6152R

increased body size | third instar larval stage, with Scer\GAL4^phtm.PU

Nadk1b^NIG.6152R

majority die during third instar larval stage, with Scer\GAL4^phtm.PU

Nadk1b^NIG.6152R

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (2)

Hsap\NADK

14 of 14

Yes

Hsap\NADK2

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (2)

Rnor\Nadk

14 of 14

Yes

Rnor\Nadk2

1 of 14

Mus musculus (laboratory mouse) (1)

Mmus\Nadk

13 of 14

Yes

Xenopus tropicalis (Western clawed frog) (2)

Xtro\nadk

8 of 13

Yes

Xtro\XB5949753

1 of 13

Yes

Danio rerio (Zebrafish) (2)

Drer\nadkb

13 of 14

Yes

Drer\nadka

6 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (2)

Cele\nadk-1

1 of 14

Yes

Cele\nadk-2

1 of 14

Yes

Anopheles gambiae (African malaria mosquito) (2)

Agam\AgaP_AGAP011122

11 of 12

Yes

Agam\AgaP_AGAP006767

1 of 12

Arabidopsis thaliana (thale-cress) (3)

Atha\NADK1

10 of 13

Yes

Atha\NADK2

8 of 13

Yes

Atha\NADK3

2 of 13

Saccharomyces cerevisiae (Brewer's yeast) (3)

Scer\UTR1

11 of 13

Yes

Scer\YEF1

11 of 13

Yes

Scer\POS5

5 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (4)

Spom\SPAC3H5.11

10 of 12

Yes

Spom\SPCC24B10.02c

7 of 12

Yes

Spom\SPAC1B1.02c

6 of 12

Yes

Spom\pos5

5 of 12

Yes

Escherichia coli (enterobacterium) (1)

Ecol\nadK

9 of 11

Yes

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Nadk1b. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (2)

Nadk1a

13 of 13

Nadk2

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Nadk1b^MB05931

exacerbates nervous system disease

modeled by Atpα^DTS1

(Talsma et al., 2014)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

NADK; NAD kinase

14 of 14

611616

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Show neighbor-neighbor interactions:

Off

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

Select Layout:

Force Directed

Circle

Concentric

Legend:

Protein

RNA

Selected Interactor(s)

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Nadk1b

two hybrid

(Hu et al., 2017.6.13)

Nadk1b - Nadk1a

experimental knowledge based

(Guruharsha et al., 2011)

Nadk1b - Vta1

two hybrid

(Hu et al., 2017.6.13)

Summary of Genetic Interactions

esyN Network Diagram

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

esyN Network Key:

Suppression

Enhancement

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Nadk1a

suppressible

Nadk1b

(Xu et al., 2021)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

62263

DroID - A comprehensive database of gene and protein interactions.

FBgn0053156

MIST (protein-protein) - An integrated Molecular Interaction Database

36507

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-68

Cytogenetic map

50B1-50B1

Sequence location

2R:13,548,244..13,553,240 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

50B1-50B1

Limits computationally determined from genome sequence between P{lacW}drk^k13809&P{EP}mip120^EP606 and P{lacW}l(2)03105^k16702&P{lacW}fl(2)d^k16105

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-68

(FlyBase, 2016)

2-72.5

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (10)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

59051

w^*; P{UAS-Nadk1b.R}3

59052

w^*; P{UAS-Nadk1b.R}2

25470

w¹¹¹⁸; Mi{ET1}Nadk1b^MB05931

14957

y¹; P{SUPor-P}Nadk1b^KG08567/CyO; ry⁵⁰⁶

62858

y¹ sc^* v¹ sev²¹; P{TRiP.HMC05331}attP40

Kyoto

114628

y^d2 w¹¹¹⁸ P{ey-FLP.N}2; P{neoFRT}42D P{SUPor-P}Nadk1b^KG08567 /CyO y⁺

NIG-Fly

6152R-4

P{NIG.6152R}4

2RG-1063

y² VhaAC39-1^cho-1 v¹; P{U6-Nadk1b.NIG.sgRNA}attP40/CyO

VDRC

v103902

P{KK114677}VIE-260B

v41825

w¹¹¹⁸; P{GD10645}v41825/TM3

Genomic Clones (9)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (291)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0053156

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0053156

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

There are two homologs of human cytosolic NADK, CG6145 and CG33156, which are adjacent in the genome suggesting they were generated by gene duplication. CG6145 (now 'Nadk1a') is broadly expressed in many tissues, while CG33156 (now 'Nadk1b') is specifically and highly expressed in the ovary.

(Xu et al., 2021)

Relationship to Other Genes

Source for database merge of

Source for merge of: CG6152 CG30063 as CG33156

(FlyBase, 1996-)

Additional comments

Source for merge of CG6152 CG30063 as CG33156 was sequence comparison ( date:021117 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: Nadk1b CG33156

(Xu et al., 2021)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

CG30063

CG33156

(National Institute of Genetics Fly Stocks, 2022-, Xu et al., 2021, Hu et al., 2017.6.13, Nakaoka et al., 2017, Hosono et al., 2015, Richardson, 2015.3.11, Talsma et al., 2014, Kwon et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Keleman et al., 2009.8.5, Perkins et al., 2009)

CG6152

NADK

(Xu et al., 2021)

Nadk1b

Name Synonyms

NAD kinase

(Nakaoka et al., 2017)

NAD kinase 1b

Secondary FlyBase IDs

FBgn0033854
FBgn0050063

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 71 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

36507

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

A1Z9F4

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

A1Z9F4

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0053156

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36507/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0053156

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0053156

FlyMine - An integrated database for Drosophila genomics

FBgn0053156

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG33156

MARRVEL_MODEL - MARRVEL (model organism gene)

36507

Linkouts

BioGRID - A database of protein and genetic interactions.

62263

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0053156

DroID - A comprehensive database of gene and protein interactions.

FBgn0053156

DRSC - Results frm RNAi screens

FBgn0053156

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0053156

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0053156

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG33156

Flygut - An atlas of the Drosophila adult midgut

FBgn0053156

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC006614

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MIST (protein-protein) - An integrated Molecular Interaction Database

36507

References (38)

The following external sites may use different assemblies or annotations than FlyBase.

-0.02

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