Gene Dmel\MtnD

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General Information

Symbol

Dmel\MtnD

Species

D. melanogaster

Name

Metallothionein D

Annotation symbol

CG33192

Feature type

protein_coding_gene

FlyBase ID

FBgn0053192

Gene Model Status

Current

Stock availability

None publicly available

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

92F1-92F1

Sequence location

3R:16,360,684..16,361,023 [+]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

The gene Metallothionein D is referred to in FlyBase by the symbol Dmel\MtnD (CG33192, FBgn0053192). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: metal ion binding. There is experimental evidence that it is involved in the biological process: response to copper ion. 8 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Metallothionein, family 5, Diptera. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of extremely low expression. Peak expression observed during early larval stages, during early pupal stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval/adult midgut, larval Malpighian tubules, adult heart. Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult hindgut, adult Malpighian tubules, larval/adult fat body, adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1631962_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of MtnD. Gene sequence location is 3R:16360684..16361023.

User Contributed Data

Beta version, feedback welcome

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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FB2013_03

Controlled Vocabulary Terms

metal ion homeostasis

cellular_component

FB2013_02

All updates

Click here to see a list of all updates to this record from FB2010_08 and on.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

92F1-92F1

Limits computationally determined from genome sequence between P{PZ}l(3)10585¹⁰⁵⁸⁵ and P{EP}Synd^EP409

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\MtnD for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Gene model reviewed during 5.47

Original gene annotation based on personal communication to FlyBase from Anand Selvaraj (FBrf0155339).

Low-frequency RNA-Seq exon junction(s) not annotated.

Mutation in sequenced strain: premature stop.

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

MtnD-RB

FBtr0308064

NM_176518

274

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

MtnD-PB

FBpp0300409

4.5

8.15

NP_788695

AAO41579

Additional Polypeptide Data & Comments

Reported size (kDa)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Metallothionein, family 5, Diptera (IPR000966)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q8I9B4

UniProtKB/TrEMBL

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval/adult midgut, larval Malpighian tubules, adult heart. Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult hindgut, adult Malpighian tubules, larval/adult fat body, adult carcass.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		3.45
Larval Midgut		3555.3
Larval Hindgut		484.2
Larval Malpighian Tubules		1544.5
Larval Fat Body		274.3
Larval Salivary Gland		15.2
Larval Trachea		17.65
Larval Carcass		25.55
Adult Head		41
Adult Eye		9.625
Adult Brain		9.2
Adult Thoracic-Abdominal Ganglion		10.2
Adult Crop		7.2
Adult Midgut		3204.2
Adult Hindgut		434.3
Adult Malpighian Tubules		113.2
Adult Fat Body		102.3
Adult Salivary Gland		29.1
Adult Heart		2102.05
Adult VirginFemale Spermatheca		69.9
Adult InseminatedFemale Spermatheca		36.2
Adult Ovary		2.8
Adult Testis		12.3
Adult Male Accessory Gland		4.3
Adult Carcass		115
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		3.45
Larval Midgut		(3555.3)
Larval Hindgut		484.2
Larval Malpighian Tubules		(1544.5)
Larval Fat Body		274.3
Larval Salivary Gland		15.2
Larval Trachea		17.65
Larval Carcass		25.55
Adult Head		41
Adult Eye		9.625
Adult Brain		9.2
Adult Thoracic-Abdominal Ganglion		10.2
Adult Crop		7.2
Adult Midgut		(3204.2)
Adult Hindgut		434.3
Adult Malpighian Tubules		113.2
Adult Fat Body		102.3
Adult Salivary Gland		29.1
Adult Heart		(2102.05)
Adult VirginFemale Spermatheca		69.9
Adult InseminatedFemale Spermatheca		36.2
Adult Ovary		2.8
Adult Testis		12.3
Adult Male Accessory Gland		4.3
Adult Carcass		115
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		3.45
Larval Midgut		(3555.3)
Larval Hindgut		484.2
Larval Malpighian Tubules		(1544.5)
Larval Fat Body		274.3
Larval Salivary Gland		15.2
Larval Trachea		17.65
Larval Carcass		25.55
Adult Head		41
Adult Eye		9.625
Adult Brain		9.2
Adult Thoracic-Abdominal Ganglion		10.2
Adult Crop		7.2
Adult Midgut		(3204.2)
Adult Hindgut		434.3
Adult Malpighian Tubules		113.2
Adult Fat Body		102.3
Adult Salivary Gland		29.1
Adult Heart		(2102.05)
Adult VirginFemale Spermatheca		69.9
Adult InseminatedFemale Spermatheca		36.2
Adult Ovary		2.8
Adult Testis		12.3
Adult Male Accessory Gland		4.3
Adult Carcass		115
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		3.45
Larval Midgut		3555.3
Larval Hindgut		484.2
Larval Malpighian Tubules		1544.5
Larval Fat Body		274.3
Larval Salivary Gland		15.2
Larval Trachea		17.65
Larval Carcass		25.55
Adult Head		41
Adult Eye		9.625
Adult Brain		9.2
Adult Thoracic-Abdominal Ganglion		10.2
Adult Crop		7.2
Adult Midgut		3204.2
Adult Hindgut		434.3
Adult Malpighian Tubules		113.2
Adult Fat Body		102.3
Adult Salivary Gland		29.1
Adult Heart		2102.05
Adult VirginFemale Spermatheca		69.9
Adult InseminatedFemale Spermatheca		36.2
Adult Ovary		2.8
Adult Testis		12.3
Adult Male Accessory Gland		4.3
Adult Carcass		115
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		3.45
Larval Midgut		3555.3
Larval Hindgut		484.2
Larval Malpighian Tubules		1544.5
Larval Fat Body		274.3
Larval Salivary Gland		15.2
Larval Trachea		17.65
Larval Carcass		25.55
Adult Head		41
Adult Eye		9.625
Adult Brain		9.2
Adult Thoracic-Abdominal Ganglion		10.2
Adult Crop		7.2
Adult Midgut		3204.2
Adult Hindgut		434.3
Adult Malpighian Tubules		113.2
Adult Fat Body		102.3
Adult Salivary Gland		29.1
Adult Heart		2102.05
Adult VirginFemale Spermatheca		69.9
Adult InseminatedFemale Spermatheca		36.2
Adult Ovary		2.8
Adult Testis		12.3
Adult Male Accessory Gland		4.3
Adult Carcass		115
Expression Level Scale		None Low Moderate High Very high

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		3.45
Larval Midgut		(3555.3)
Larval Hindgut		484.2
Larval Malpighian Tubules		(1544.5)
Larval Fat Body		274.3
Larval Salivary Gland		15.2
Larval Trachea		17.65
Larval Carcass		25.55
Adult Head		41
Adult Eye		9.625
Adult Brain		9.2
Adult Thoracic-Abdominal Ganglion		10.2
Adult Crop		7.2
Adult Midgut		(3204.2)
Adult Hindgut		434.3
Adult Malpighian Tubules		113.2
Adult Fat Body		102.3
Adult Salivary Gland		29.1
Adult Heart		(2102.05)
Adult VirginFemale Spermatheca		69.9
Adult InseminatedFemale Spermatheca		36.2
Adult Ovary		2.8
Adult Testis		12.3
Adult Male Accessory Gland		4.3
Adult Carcass		115
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		3.45
Larval Midgut		(3555.3)
Larval Hindgut		484.2
Larval Malpighian Tubules		1544.5
Larval Fat Body		274.3
Larval Salivary Gland		15.2
Larval Trachea		17.65
Larval Carcass		25.55
Adult Head		41
Adult Eye		9.625
Adult Brain		9.2
Adult Thoracic-Abdominal Ganglion		10.2
Adult Crop		7.2
Adult Midgut		(3204.2)
Adult Hindgut		434.3
Adult Malpighian Tubules		113.2
Adult Fat Body		102.3
Adult Salivary Gland		29.1
Adult Heart		(2102.05)
Adult VirginFemale Spermatheca		69.9
Adult InseminatedFemale Spermatheca		36.2
Adult Ovary		2.8
Adult Testis		12.3
Adult Male Accessory Gland		4.3
Adult Carcass		115
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		3.45
Larval Midgut		3555.3
Larval Hindgut		484.2
Larval Malpighian Tubules		1544.5
Larval Fat Body		274.3
Larval Salivary Gland		15.2
Larval Trachea		17.65
Larval Carcass		25.55
Adult Head		41
Adult Eye		9.625
Adult Brain		9.2
Adult Thoracic-Abdominal Ganglion		10.2
Adult Crop		7.2
Adult Midgut		3204.2
Adult Hindgut		434.3
Adult Malpighian Tubules		113.2
Adult Fat Body		102.3
Adult Salivary Gland		29.1
Adult Heart		2102.05
Adult VirginFemale Spermatheca		69.9
Adult InseminatedFemale Spermatheca		36.2
Adult Ovary		2.8
Adult Testis		12.3
Adult Male Accessory Gland		4.3
Adult Carcass		115
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	41
NA	Eye	9.625
NA	Brain	9.2
3.45	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	no informative data
NA	Crop	7.2
3555.3	Midgut	3204.2
484.2	Hindgut	434.3
1544.5	Malpighian Tubules	113.2
274.3	Fat Body	102.3
no informative data	Salivary Gland	no informative data
NA	Heart	2102.05
no informative data	Trachea	NA
NA	VirginFemale Spermatheca	69.9
NA	InseminatedFemale Spermatheca	36.2
NA	Ovary	2.8
NA	Testis	no informative data
NA	Male Accessory Gland	4.3
25.55	Carcass	115

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		116
central nervous system, larvae L3		1
central nervous system, pupae P8		8
head, virgin 1-day female		30
head, virgin 4-day female		10
head, virgin 20-day female		47
head, mated 1-day female		18
head, mated 4-day female		9
head, mated 20-day female		58
head, mated 1-day male		25
head, mated 4-day male		18
head, mated 20-day male		15
salivary gland, larvae L3 wandering		9
salivary gland, white prepupae		11
digestive system, larvae L3 wandering		1074
digestive system, 1-day adult		2744
digestive system, 4-day adult		2463
digestive system, 20-day adult		982
fat body, larvae L3 wandering		12
fat body, white prepupae		62
fat body, pupae P8		298
carcass, larvae L3 wandering		6
carcass, 1-day adult		72
carcass, 4-day adult		30
carcass, 20-day adult		106
ovary, virgin 4-day female		1
ovary, mated 4-day female		4
testis, mated 4-day male		20
accessory gland, mated 4-day male		34
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(116)
central nervous system, larvae L3		1
central nervous system, pupae P8		8
head, virgin 1-day female		(30)
head, virgin 4-day female		10
head, virgin 20-day female		(47)
head, mated 1-day female		18
head, mated 4-day female		9
head, mated 20-day female		(58)
head, mated 1-day male		25
head, mated 4-day male		18
head, mated 20-day male		15
salivary gland, larvae L3 wandering		9
salivary gland, white prepupae		11
digestive system, larvae L3 wandering		(1074)
digestive system, 1-day adult		(2744)
digestive system, 4-day adult		(2463)
digestive system, 20-day adult		(982)
fat body, larvae L3 wandering		12
fat body, white prepupae		(62)
fat body, pupae P8		(298)
carcass, larvae L3 wandering		6
carcass, 1-day adult		(72)
carcass, 4-day adult		(30)
carcass, 20-day adult		(106)
ovary, virgin 4-day female		1
ovary, mated 4-day female		4
testis, mated 4-day male		20
accessory gland, mated 4-day male		(34)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(116)
central nervous system, larvae L3		1
central nervous system, pupae P8		8
head, virgin 1-day female		30
head, virgin 4-day female		10
head, virgin 20-day female		47
head, mated 1-day female		18
head, mated 4-day female		9
head, mated 20-day female		58
head, mated 1-day male		25
head, mated 4-day male		18
head, mated 20-day male		15
salivary gland, larvae L3 wandering		9
salivary gland, white prepupae		11
digestive system, larvae L3 wandering		(1074)
digestive system, 1-day adult		(2744)
digestive system, 4-day adult		(2463)
digestive system, 20-day adult		(982)
fat body, larvae L3 wandering		12
fat body, white prepupae		62
fat body, pupae P8		(298)
carcass, larvae L3 wandering		6
carcass, 1-day adult		72
carcass, 4-day adult		30
carcass, 20-day adult		106
ovary, virgin 4-day female		1
ovary, mated 4-day female		4
testis, mated 4-day male		20
accessory gland, mated 4-day male		34
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		116
central nervous system, larvae L3		1
central nervous system, pupae P8		8
head, virgin 1-day female		30
head, virgin 4-day female		10
head, virgin 20-day female		47
head, mated 1-day female		18
head, mated 4-day female		9
head, mated 20-day female		58
head, mated 1-day male		25
head, mated 4-day male		18
head, mated 20-day male		15
salivary gland, larvae L3 wandering		9
salivary gland, white prepupae		11
digestive system, larvae L3 wandering		1074
digestive system, 1-day adult		2744
digestive system, 4-day adult		2463
digestive system, 20-day adult		982
fat body, larvae L3 wandering		12
fat body, white prepupae		62
fat body, pupae P8		298
carcass, larvae L3 wandering		6
carcass, 1-day adult		72
carcass, 4-day adult		30
carcass, 20-day adult		106
ovary, virgin 4-day female		1
ovary, mated 4-day female		4
testis, mated 4-day male		20
accessory gland, mated 4-day male		34
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		116
central nervous system, larvae L3		1
central nervous system, pupae P8		8
head, virgin 1-day female		30
head, virgin 4-day female		10
head, virgin 20-day female		47
head, mated 1-day female		18
head, mated 4-day female		9
head, mated 20-day female		58
head, mated 1-day male		25
head, mated 4-day male		18
head, mated 20-day male		15
salivary gland, larvae L3 wandering		9
salivary gland, white prepupae		11
digestive system, larvae L3 wandering		1074
digestive system, 1-day adult		2744
digestive system, 4-day adult		2463
digestive system, 20-day adult		982
fat body, larvae L3 wandering		12
fat body, white prepupae		62
fat body, pupae P8		298
carcass, larvae L3 wandering		6
carcass, 1-day adult		72
carcass, 4-day adult		30
carcass, 20-day adult		106
ovary, virgin 4-day female		1
ovary, mated 4-day female		4
testis, mated 4-day male		20
accessory gland, mated 4-day male		34
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(116)
central nervous system, larvae L3		1
central nervous system, pupae P8		8
head, virgin 1-day female		30
head, virgin 4-day female		10
head, virgin 20-day female		(47)
head, mated 1-day female		18
head, mated 4-day female		9
head, mated 20-day female		(58)
head, mated 1-day male		25
head, mated 4-day male		18
head, mated 20-day male		15
salivary gland, larvae L3 wandering		9
salivary gland, white prepupae		11
digestive system, larvae L3 wandering		(1074)
digestive system, 1-day adult		(2744)
digestive system, 4-day adult		(2463)
digestive system, 20-day adult		(982)
fat body, larvae L3 wandering		12
fat body, white prepupae		(62)
fat body, pupae P8		(298)
carcass, larvae L3 wandering		6
carcass, 1-day adult		(72)
carcass, 4-day adult		30
carcass, 20-day adult		(106)
ovary, virgin 4-day female		1
ovary, mated 4-day female		4
testis, mated 4-day male		20
accessory gland, mated 4-day male		(34)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		116
central nervous system, larvae L3		1
central nervous system, pupae P8		8
head, virgin 1-day female		30
head, virgin 4-day female		10
head, virgin 20-day female		47
head, mated 1-day female		18
head, mated 4-day female		9
head, mated 20-day female		58
head, mated 1-day male		25
head, mated 4-day male		18
head, mated 20-day male		15
salivary gland, larvae L3 wandering		9
salivary gland, white prepupae		11
digestive system, larvae L3 wandering		(1074)
digestive system, 1-day adult		(2744)
digestive system, 4-day adult		(2463)
digestive system, 20-day adult		(982)
fat body, larvae L3 wandering		12
fat body, white prepupae		62
fat body, pupae P8		(298)
carcass, larvae L3 wandering		6
carcass, 1-day adult		72
carcass, 4-day adult		30
carcass, 20-day adult		106
ovary, virgin 4-day female		1
ovary, mated 4-day female		4
testis, mated 4-day male		20
accessory gland, mated 4-day male		34
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		116
central nervous system, larvae L3		1
central nervous system, pupae P8		8
head, virgin 1-day female		30
head, virgin 4-day female		10
head, virgin 20-day female		47
head, mated 1-day female		18
head, mated 4-day female		9
head, mated 20-day female		58
head, mated 1-day male		25
head, mated 4-day male		18
head, mated 20-day male		15
salivary gland, larvae L3 wandering		9
salivary gland, white prepupae		11
digestive system, larvae L3 wandering		1074
digestive system, 1-day adult		2744
digestive system, 4-day adult		2463
digestive system, 20-day adult		982
fat body, larvae L3 wandering		12
fat body, white prepupae		62
fat body, pupae P8		298
carcass, larvae L3 wandering		6
carcass, 1-day adult		72
carcass, 4-day adult		30
carcass, 20-day adult		106
ovary, virgin 4-day female		1
ovary, mated 4-day female		4
testis, mated 4-day male		20
accessory gland, mated 4-day male		34
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of extremely low expression. Peak expression observed during early larval stages, during early pupal stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		0
embryo 16-18hr		7
embryo 18-20hr		26
embryo 20-22hr		90
embryo 22-24hr		96
larva L1		165
larva L2		80
larva L3 12hr old		98
larva L3 puffstage 1-2		90
larva L3 puffstage 3-6		74
larva L3 puffstage 7-9		99
white prepupae new		168
white prepupae 12hr		111
white prepupae 24hr		125
pupae 2d postWPP		42
pupae 3d postWPP		27
pupae 4d postWPP		18
adult male 01day		15
adult male 05day		6
adult male 30day		16
adult female 01day		7
adult female 05day		13
adult female 30day		25
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		0
embryo 16-18hr		7
embryo 18-20hr		26
embryo 20-22hr		(90)
embryo 22-24hr		(96)
larva L1		(165)
larva L2		(80)
larva L3 12hr old		(98)
larva L3 puffstage 1-2		(90)
larva L3 puffstage 3-6		(74)
larva L3 puffstage 7-9		(99)
white prepupae new		(168)
white prepupae 12hr		(111)
white prepupae 24hr		(125)
pupae 2d postWPP		(42)
pupae 3d postWPP		(27)
pupae 4d postWPP		18
adult male 01day		15
adult male 05day		6
adult male 30day		16
adult female 01day		7
adult female 05day		13
adult female 30day		25
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		0
embryo 16-18hr		7
embryo 18-20hr		26
embryo 20-22hr		90
embryo 22-24hr		96
larva L1		(165)
larva L2		80
larva L3 12hr old		98
larva L3 puffstage 1-2		90
larva L3 puffstage 3-6		74
larva L3 puffstage 7-9		99
white prepupae new		(168)
white prepupae 12hr		(111)
white prepupae 24hr		(125)
pupae 2d postWPP		42
pupae 3d postWPP		27
pupae 4d postWPP		18
adult male 01day		15
adult male 05day		6
adult male 30day		16
adult female 01day		7
adult female 05day		13
adult female 30day		25
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		0
embryo 16-18hr		7
embryo 18-20hr		26
embryo 20-22hr		90
embryo 22-24hr		96
larva L1		165
larva L2		80
larva L3 12hr old		98
larva L3 puffstage 1-2		90
larva L3 puffstage 3-6		74
larva L3 puffstage 7-9		99
white prepupae new		168
white prepupae 12hr		111
white prepupae 24hr		125
pupae 2d postWPP		42
pupae 3d postWPP		27
pupae 4d postWPP		18
adult male 01day		15
adult male 05day		6
adult male 30day		16
adult female 01day		7
adult female 05day		13
adult female 30day		25
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		0
embryo 16-18hr		7
embryo 18-20hr		26
embryo 20-22hr		90
embryo 22-24hr		96
larva L1		165
larva L2		80
larva L3 12hr old		98
larva L3 puffstage 1-2		90
larva L3 puffstage 3-6		74
larva L3 puffstage 7-9		99
white prepupae new		168
white prepupae 12hr		111
white prepupae 24hr		125
pupae 2d postWPP		42
pupae 3d postWPP		27
pupae 4d postWPP		18
adult male 01day		15
adult male 05day		6
adult male 30day		16
adult female 01day		7
adult female 05day		13
adult female 30day		25
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		0
embryo 16-18hr		7
embryo 18-20hr		26
embryo 20-22hr		(90)
embryo 22-24hr		(96)
larva L1		(165)
larva L2		(80)
larva L3 12hr old		(98)
larva L3 puffstage 1-2		(90)
larva L3 puffstage 3-6		(74)
larva L3 puffstage 7-9		(99)
white prepupae new		(168)
white prepupae 12hr		(111)
white prepupae 24hr		(125)
pupae 2d postWPP		(42)
pupae 3d postWPP		27
pupae 4d postWPP		18
adult male 01day		15
adult male 05day		6
adult male 30day		16
adult female 01day		7
adult female 05day		13
adult female 30day		25
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		0
embryo 16-18hr		7
embryo 18-20hr		26
embryo 20-22hr		90
embryo 22-24hr		96
larva L1		(165)
larva L2		80
larva L3 12hr old		98
larva L3 puffstage 1-2		90
larva L3 puffstage 3-6		74
larva L3 puffstage 7-9		99
white prepupae new		(168)
white prepupae 12hr		111
white prepupae 24hr		125
pupae 2d postWPP		42
pupae 3d postWPP		27
pupae 4d postWPP		18
adult male 01day		15
adult male 05day		6
adult male 30day		16
adult female 01day		7
adult female 05day		13
adult female 30day		25
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		0
embryo 16-18hr		7
embryo 18-20hr		26
embryo 20-22hr		90
embryo 22-24hr		96
larva L1		165
larva L2		80
larva L3 12hr old		98
larva L3 puffstage 1-2		90
larva L3 puffstage 3-6		74
larva L3 puffstage 7-9		99
white prepupae new		168
white prepupae 12hr		111
white prepupae 24hr		125
pupae 2d postWPP		42
pupae 3d postWPP		27
pupae 4d postWPP		18
adult male 01day		15
adult male 05day		6
adult male 30day		16
adult female 01day		7
adult female 05day		13
adult female 30day		25
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		1
Schneider line 2 Sg4		5
embryonic 1182-4H		0
embryonic GM2		8
embryonic Kc167		9
embryonic S1		0
embryonic S3		0
leg disc CME L1		5
wing disc CME-W2		13
wing disc ML-DmD8		0
wing disc ML-DmD9		0
wing disc ML-DmD16-c3		1
wing disc ML-DmD21		0
wing disc ML-DmD32		11
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		0
antennal disc ML-DmD20-c5		3
mixed discs ML-DmD4-c1		136
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		0
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		1
Schneider line 2 Sg4		5
embryonic 1182-4H		0
embryonic GM2		8
embryonic Kc167		9
embryonic S1		0
embryonic S3		0
leg disc CME L1		5
wing disc CME-W2		13
wing disc ML-DmD8		0
wing disc ML-DmD9		0
wing disc ML-DmD16-c3		1
wing disc ML-DmD21		0
wing disc ML-DmD32		11
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		0
antennal disc ML-DmD20-c5		3
mixed discs ML-DmD4-c1		(136)
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		0
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		0
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		1
Schneider line 2 Sg4		5
embryonic 1182-4H		0
embryonic GM2		8
embryonic Kc167		9
embryonic S1		0
embryonic S3		0
leg disc CME L1		5
wing disc CME-W2		13
wing disc ML-DmD8		0
wing disc ML-DmD9		0
wing disc ML-DmD16-c3		1
wing disc ML-DmD21		0
wing disc ML-DmD32		11
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		0
antennal disc ML-DmD20-c5		3
mixed discs ML-DmD4-c1		(136)
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		0
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		1
Schneider line 2 Sg4		5
embryonic 1182-4H		0
embryonic GM2		8
embryonic Kc167		9
embryonic S1		0
embryonic S3		0
leg disc CME L1		5
wing disc CME-W2		13
wing disc ML-DmD8		0
wing disc ML-DmD9		0
wing disc ML-DmD16-c3		1
wing disc ML-DmD21		0
wing disc ML-DmD32		11
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		0
antennal disc ML-DmD20-c5		3
mixed discs ML-DmD4-c1		136
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		0
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		0
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		1
Schneider line 2 Sg4		5
embryonic 1182-4H		0
embryonic GM2		8
embryonic Kc167		9
embryonic S1		0
embryonic S3		0
leg disc CME L1		5
wing disc CME-W2		13
wing disc ML-DmD8		0
wing disc ML-DmD9		0
wing disc ML-DmD16-c3		1
wing disc ML-DmD21		0
wing disc ML-DmD32		11
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		0
antennal disc ML-DmD20-c5		3
mixed discs ML-DmD4-c1		136
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		0
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		1
Schneider line 2 Sg4		5
embryonic 1182-4H		0
embryonic GM2		8
embryonic Kc167		9
embryonic S1		0
embryonic S3		0
leg disc CME L1		5
wing disc CME-W2		13
wing disc ML-DmD8		0
wing disc ML-DmD9		0
wing disc ML-DmD16-c3		1
wing disc ML-DmD21		0
wing disc ML-DmD32		11
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		0
antennal disc ML-DmD20-c5		3
mixed discs ML-DmD4-c1		(136)
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		0
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		0
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		1
Schneider line 2 Sg4		5
embryonic 1182-4H		0
embryonic GM2		8
embryonic Kc167		9
embryonic S1		0
embryonic S3		0
leg disc CME L1		5
wing disc CME-W2		13
wing disc ML-DmD8		0
wing disc ML-DmD9		0
wing disc ML-DmD16-c3		1
wing disc ML-DmD21		0
wing disc ML-DmD32		11
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		0
antennal disc ML-DmD20-c5		3
mixed discs ML-DmD4-c1		136
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		0
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		1
Schneider line 2 Sg4		5
embryonic 1182-4H		0
embryonic GM2		8
embryonic Kc167		9
embryonic S1		0
embryonic S3		0
leg disc CME L1		5
wing disc CME-W2		13
wing disc ML-DmD8		0
wing disc ML-DmD9		0
wing disc ML-DmD16-c3		1
wing disc ML-DmD21		0
wing disc ML-DmD32		11
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		0
antennal disc ML-DmD20-c5		3
mixed discs ML-DmD4-c1		136
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		0
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		77
cold shock, 4-day adult		85
heat shock, 4-day adult		1284
Cadmium 50 mM 6 hrs, larvae L3		9028
Cadmium 50 mM 12 hrs, larvae L3		10662
Cadmium 50 mM 48 hrs, 4-day adult		2505
Cadmium 100 mM 48 hrs, 4-day adult		2807
Copper 0.5 mM 12 hrs, larvae L3		3667
Copper 15 mM 48 hrs, 4-day adult		7482
Zinc 5 mM 12 hrs, larvae L3		1861
Zinc 4.5 mM 48 hrs, 4-day adult		3026
Ethanol 2.5% 3 hrs, larvae L3		982
Ethanol 5% 3 hrs, larvae L3		2008
Ethanol 10% 3 hrs, larvae L3		1547
Caffeine 1.5 mg/ml 4 hrs, larvae L3		1292
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		118
Caffeine 25 mg/ml 48 hrs, 4-day adult		195
Paraquat 5 mM 48 hrs, 4-day adult		33
Paraquat 10 mM 48 hrs, 4-day adult		76
Rotenone 2 μg 12 hrs, larvae L3		4122
Rotenone 8 μg 12 hrs, larvae L3		5219
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		(77)
cold shock, 4-day adult		(85)
heat shock, 4-day adult		(1284)
Cadmium 50 mM 6 hrs, larvae L3		(9028)
Cadmium 50 mM 12 hrs, larvae L3		(10662)
Cadmium 50 mM 48 hrs, 4-day adult		(2505)
Cadmium 100 mM 48 hrs, 4-day adult		(2807)
Copper 0.5 mM 12 hrs, larvae L3		(3667)
Copper 15 mM 48 hrs, 4-day adult		(7482)
Zinc 5 mM 12 hrs, larvae L3		(1861)
Zinc 4.5 mM 48 hrs, 4-day adult		(3026)
Ethanol 2.5% 3 hrs, larvae L3		(982)
Ethanol 5% 3 hrs, larvae L3		(2008)
Ethanol 10% 3 hrs, larvae L3		(1547)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(1292)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(118)
Caffeine 25 mg/ml 48 hrs, 4-day adult		(195)
Paraquat 5 mM 48 hrs, 4-day adult		(33)
Paraquat 10 mM 48 hrs, 4-day adult		(76)
Rotenone 2 μg 12 hrs, larvae L3		(4122)
Rotenone 8 μg 12 hrs, larvae L3		(5219)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		77
cold shock, 4-day adult		85
heat shock, 4-day adult		(1284)
Cadmium 50 mM 6 hrs, larvae L3		(9028)
Cadmium 50 mM 12 hrs, larvae L3		(10662)
Cadmium 50 mM 48 hrs, 4-day adult		(2505)
Cadmium 100 mM 48 hrs, 4-day adult		(2807)
Copper 0.5 mM 12 hrs, larvae L3		(3667)
Copper 15 mM 48 hrs, 4-day adult		(7482)
Zinc 5 mM 12 hrs, larvae L3		(1861)
Zinc 4.5 mM 48 hrs, 4-day adult		(3026)
Ethanol 2.5% 3 hrs, larvae L3		(982)
Ethanol 5% 3 hrs, larvae L3		(2008)
Ethanol 10% 3 hrs, larvae L3		(1547)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(1292)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(118)
Caffeine 25 mg/ml 48 hrs, 4-day adult		(195)
Paraquat 5 mM 48 hrs, 4-day adult		33
Paraquat 10 mM 48 hrs, 4-day adult		76
Rotenone 2 μg 12 hrs, larvae L3		(4122)
Rotenone 8 μg 12 hrs, larvae L3		(5219)
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		77
cold shock, 4-day adult		85
heat shock, 4-day adult		1284
Cadmium 50 mM 6 hrs, larvae L3		9028
Cadmium 50 mM 12 hrs, larvae L3		10662
Cadmium 50 mM 48 hrs, 4-day adult		2505
Cadmium 100 mM 48 hrs, 4-day adult		2807
Copper 0.5 mM 12 hrs, larvae L3		3667
Copper 15 mM 48 hrs, 4-day adult		7482
Zinc 5 mM 12 hrs, larvae L3		1861
Zinc 4.5 mM 48 hrs, 4-day adult		3026
Ethanol 2.5% 3 hrs, larvae L3		982
Ethanol 5% 3 hrs, larvae L3		2008
Ethanol 10% 3 hrs, larvae L3		1547
Caffeine 1.5 mg/ml 4 hrs, larvae L3		1292
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		118
Caffeine 25 mg/ml 48 hrs, 4-day adult		195
Paraquat 5 mM 48 hrs, 4-day adult		33
Paraquat 10 mM 48 hrs, 4-day adult		76
Rotenone 2 μg 12 hrs, larvae L3		4122
Rotenone 8 μg 12 hrs, larvae L3		5219
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		77
cold shock, 4-day adult		85
heat shock, 4-day adult		1284
Cadmium 50 mM 6 hrs, larvae L3		9028
Cadmium 50 mM 12 hrs, larvae L3		10662
Cadmium 50 mM 48 hrs, 4-day adult		2505
Cadmium 100 mM 48 hrs, 4-day adult		2807
Copper 0.5 mM 12 hrs, larvae L3		3667
Copper 15 mM 48 hrs, 4-day adult		7482
Zinc 5 mM 12 hrs, larvae L3		1861
Zinc 4.5 mM 48 hrs, 4-day adult		3026
Ethanol 2.5% 3 hrs, larvae L3		982
Ethanol 5% 3 hrs, larvae L3		2008
Ethanol 10% 3 hrs, larvae L3		1547
Caffeine 1.5 mg/ml 4 hrs, larvae L3		1292
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		118
Caffeine 25 mg/ml 48 hrs, 4-day adult		195
Paraquat 5 mM 48 hrs, 4-day adult		33
Paraquat 10 mM 48 hrs, 4-day adult		76
Rotenone 2 μg 12 hrs, larvae L3		4122
Rotenone 8 μg 12 hrs, larvae L3		5219
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		(77)
cold shock, 4-day adult		(85)
heat shock, 4-day adult		(1284)
Cadmium 50 mM 6 hrs, larvae L3		(9028)
Cadmium 50 mM 12 hrs, larvae L3		(10662)
Cadmium 50 mM 48 hrs, 4-day adult		(2505)
Cadmium 100 mM 48 hrs, 4-day adult		(2807)
Copper 0.5 mM 12 hrs, larvae L3		(3667)
Copper 15 mM 48 hrs, 4-day adult		(7482)
Zinc 5 mM 12 hrs, larvae L3		(1861)
Zinc 4.5 mM 48 hrs, 4-day adult		(3026)
Ethanol 2.5% 3 hrs, larvae L3		(982)
Ethanol 5% 3 hrs, larvae L3		(2008)
Ethanol 10% 3 hrs, larvae L3		(1547)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(1292)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(118)
Caffeine 25 mg/ml 48 hrs, 4-day adult		(195)
Paraquat 5 mM 48 hrs, 4-day adult		(33)
Paraquat 10 mM 48 hrs, 4-day adult		(76)
Rotenone 2 μg 12 hrs, larvae L3		(4122)
Rotenone 8 μg 12 hrs, larvae L3		(5219)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		77
cold shock, 4-day adult		85
heat shock, 4-day adult		(1284)
Cadmium 50 mM 6 hrs, larvae L3		(9028)
Cadmium 50 mM 12 hrs, larvae L3		(10662)
Cadmium 50 mM 48 hrs, 4-day adult		(2505)
Cadmium 100 mM 48 hrs, 4-day adult		(2807)
Copper 0.5 mM 12 hrs, larvae L3		(3667)
Copper 15 mM 48 hrs, 4-day adult		(7482)
Zinc 5 mM 12 hrs, larvae L3		(1861)
Zinc 4.5 mM 48 hrs, 4-day adult		(3026)
Ethanol 2.5% 3 hrs, larvae L3		(982)
Ethanol 5% 3 hrs, larvae L3		(2008)
Ethanol 10% 3 hrs, larvae L3		(1547)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(1292)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		118
Caffeine 25 mg/ml 48 hrs, 4-day adult		(195)
Paraquat 5 mM 48 hrs, 4-day adult		33
Paraquat 10 mM 48 hrs, 4-day adult		76
Rotenone 2 μg 12 hrs, larvae L3		(4122)
Rotenone 8 μg 12 hrs, larvae L3		(5219)
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		77
cold shock, 4-day adult		85
heat shock, 4-day adult		1284
Cadmium 50 mM 6 hrs, larvae L3		9028
Cadmium 50 mM 12 hrs, larvae L3		10662
Cadmium 50 mM 48 hrs, 4-day adult		2505
Cadmium 100 mM 48 hrs, 4-day adult		2807
Copper 0.5 mM 12 hrs, larvae L3		3667
Copper 15 mM 48 hrs, 4-day adult		7482
Zinc 5 mM 12 hrs, larvae L3		1861
Zinc 4.5 mM 48 hrs, 4-day adult		3026
Ethanol 2.5% 3 hrs, larvae L3		982
Ethanol 5% 3 hrs, larvae L3		2008
Ethanol 10% 3 hrs, larvae L3		1547
Caffeine 1.5 mg/ml 4 hrs, larvae L3		1292
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		118
Caffeine 25 mg/ml 48 hrs, 4-day adult		195
Paraquat 5 mM 48 hrs, 4-day adult		33
Paraquat 10 mM 48 hrs, 4-day adult		76
Rotenone 2 μg 12 hrs, larvae L3		4122
Rotenone 8 μg 12 hrs, larvae L3		5219
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010)

mE2_34_mRNA_expression_cluster_18

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0053192

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG33192-RA

Alleles & Phenotypes

Summary of Allele Phenotypes

Phenotype manifest in

Allele

Classical Alleles ( 3 )

For All Classical Alleles Show

Allele of MtnD	Mutagen	Known lesion
MtnD^dsRed	FLPase recombination SCEI endonuclease I-CreI endonuclease gene targeting by homologous recombination	Yes
MtnD^OreR	spontaneous	Yes
MtnD^ΔATG	FLPase recombination SCEI endonuclease I-CreI endonuclease gene targeting by homologous recombination	Yes

Alleles Carried on Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Allele of MtnD	Mutagen	Known lesion
MtnD^{dsRed.Scer\SceI.RS}	in vitro construct	Yes
MtnD^Scer\UAS.cEa	in vitro construct - regulatory fusion	Yes
MtnD^T:Avic\GFP	in vitro construct - coding region fusion	Yes
MtnD^{T:Disc\RFP-mCherry}	in vitro construct - coding region fusion	Yes
MtnD^{ΔATG.Scer\SceI.RS}	in vitro construct	Yes

Aneuploid Aberrations

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{UAS-MtnD.E}

characterization construct

P{(FRT.w^3xP3.MtnD^{dsRed.Scer\SceI.RS})}

P{(FRT.w^3xP3.MtnD^{ΔATG.Scer\SceI.RS})}

P{MtnD.GFP}

P{MtnD.mCherry}

Insertions

Type of insertions

Name

Expression data

Gene Ontology: Function, Process & Cellular Component ( 4 unique terms )

There is no information supporting a cellular component term for this gene.

(Based on a review of available data on: 2013-03-07)

Terms Based on Experimental Evidence ( 4 terms )

Molecular Function

CV term

Evidence

References

metal ion binding

inferred from direct assay

(Pérez-Rafael et al., 2012)

Biological Process

CV term

Evidence

References

metal ion homeostasis

inferred from mutant phenotype

(Egli et al., 2006)

response to copper ion

inferred from direct assay

(Atanesyan et al., 2011)

response to metal ion

inferred from direct assay

(Egli et al., 2006)

Cellular Component ( 0 terms)

Terms Based on Predictions or Assertions ( 1 term )

Molecular Function ( 0 terms)

Biological Process

CV term

Evidence

References

response to metal ion

inferred from sequence or structural similarity with MtnE

(Atanesyan et al., 2011)

Cellular Component ( 0 terms)

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

MtnD allele

Gene

References

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0053192

Orthologs

OrthoDB Orthologs (12) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG6J3X3X

Dipteran inclusive ortholog search

EOG69S6H5

Insect inclusive ortholog search

No orthologs identified

Arthropod inclusive ortholog search

No orthologs identified

Metazoa inclusive ortholog search

No orthologs identified

Orthologs in Drosophila Species (EOG6J3X3X)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila pseudoobscura pseudoobscura

Dpse\MtnD

Drosophila persimilis

Dper\MtnD

Drosophila willistoni

Dwil\MtnD

Drosophila virilis

Dvir\MtnD

Drosophila grimshawi

Dgri\MtnC

Orthologs in non-Drosophila Dipterans (EOG69S6H5)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL008176

Anopheles gambiae

Malaria mosquito

Agam\AGAP001890

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ008379

Orthologs in non-Dipteran Insects (None identified)

No non-Dipteran orthologies identified

Orthologs in non-Insect Arthropods (None identified)

No non-Insect Arthropod orthologies identified

Orthologs in non-Arthropod Metazoa (None identified)

No non-Arthropod Metazoa orthologies identified

Human Orthologs (0)

Gene

OMIM

HGNC

AAA Orthologs (0) based on analysis using Dmel annotation version 4.3

No orthologs identified

Stocks & Reagents

Stocks Listed in FlyBase ( 0 )

Genomic Clones ( 2 )

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 4 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Linkouts

DRSC - Results from RNAi screens.

•

FBgn0053192

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

326270

Antibody Information

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for identity of: MtnD CG33192

(FlyBase, 1996-)

Source for database merge of

Additional comments

Source for identity of MtnD CG33192 was sequence comparison (date:021205).

(FlyBase, 1996-)

Other Comments

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_176518

RefSeq (Proteins)

•

NP_788695

DDBJ / EMBL / GenBank

•

Entrez Gene - A searchable database of RefSeq genes.

•

326270

UniProtKB/Swiss-Prot

•

Q8I9B4

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Metallothionein, family 5, Diptera (IPR000966)

Linkouts

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0053192

DRSC - Results from RNAi screens.

•

FBgn0053192

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0053192

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG33192-RA

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0053192

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

326270

modMine - Data generated by the modENCODE project.

•

FBgn0053192

Synonyms & Secondary IDs ( 6 )

Reported As

Symbol Synonym

CG33192

(Herranz et al., 2012)

MtnD

(Marr et al., 2006, Egli et al., 2006, Pérez-Rafael et al., 2012, Egli et al., 2006, Egli et al., 2003, Yepiskoposyan et al., 2006, Jungreis, 2011.11.18, Telonis-Scott et al., 2009, Wang et al., 2009, Chamilos et al., 2008, Adryan et al., 2007, Cherbas et al., 2011, Guirola et al., 2011, Atanesyan et al., 2011)

mtnD

(Kappeler et al., 2008)

unnamed

(Selvaraj, 2002.9.15)

Name Synonym

metallothionein

(Balamurugan et al., 2007)

Metallothionein D

(Zhang et al., 2011)

Secondary FlyBase IDs

References ( 26 )

Generate a list of

All references relating to this gene ( 26 )

List References by type

Recent research papers ( 7 )

Günther et al., 2012, Int. J. Biochem. & Cell Biol. 44(2): 404--411: Dissection of Drosophila MTF-1 reveals a domain for differential target gene activation upon copper overload vs. copper starvation. [FBrf0217154]
Herranz et al., 2012, BMC Genomics 13: 52: Microgravity simulation by diamagnetic levitation: effects of a strong gradient magnetic field on the transcriptional profile of Drosophila melanogaster. [FBrf0217750]
Pérez-Rafael et al., 2012, Metallomics 4(4): 342--349: Is MtnE, the fifth Drosophila metallothionein, functionally distinct from the other members of this polymorphic protein family? [FBrf0217892]
Atanesyan et al., 2011, J. Biol. Inorg. Chem. 16(7): 1047--1056: Characterization of MtnE, the fifth metallothionein member in Drosophila. [FBrf0215591]
Cherbas et al., 2011, Genome Res. 21(2): 301--314: The transcriptional diversity of 25 Drosophila cell lines. [FBrf0213077]
Guirola et al., 2011, J. Inorg. Biochem. 105(8): 1050--1059: Comparative genomics analysis of metallothioneins in twelve Drosophila species. [FBrf0214507]
Zhang et al., 2011, J. Exp. Biol. 214(23): 4021--4029: Divergent transcriptomic responses to repeated and single cold exposures in Drosophila melanogaster. [FBrf0216629]

Show all research papers ...