General Information
Symbol
Dmel\Mical
Species
D. melanogaster
Name
Molecule interacting with CasL
Annotation Symbol
CG33208
Feature Type
FlyBase ID
FBgn0053208
Gene Model Status
Stock Availability
Gene Snapshot
Molecule interacting with CasL (Mical) encodes a multi-domain flavoprotein monooxygenase (Redox) enzyme. It is activated by actin filaments, and then oxidizes actin on methionines 44 and 47, disassembling the actin filaments and inhibiting actin polymerization. This regulates cell morphology in the nervous system and muscle, working with the the receptor encoded by PlexA and cofilin, encoded by tsr. [Date last reviewed: 2018-01-25]
Genomic Location
Cytogenetic map
Sequence location
3R:10,001,554..10,042,895 [-]
Recombination map
3-49
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
GO Summary Ribbons
Families, Domains and Molecular Function
Protein Family (UniProt, Sequence Similarities)
Belongs to the Mical family. (Q86BA1)
Summaries
Gene Group Membership
PAIRED DONOR OXIDOREDUCTASES, INCORPORATION OF MOLECULAR OXYGEN, NAD(P)H AS DONOR -
Paired donor oxidoreductases, incorporation of molecular oxygen, NAD(P)H as donor, include dehydrogenases that catalyze the oxidation reduction reaction in which hydrogen or electrons are transferred from NADH or NADPH and one other donor, and one atom of oxygen is incorporated into one donor.
UniProt Contributed Function Data
Monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin. Acts by modifying actin subunits at 'Met-44' and 'Met-47' through the addition of oxygen to form methionine-sulfoxide, leading to promote actin filament disassembly and prevent repolymerization. Plays a key role in semaphorin-plexin repulsive axon guidance and cell morphological changes, probably via its ability to modify and regulate actin.
(UniProt, Q86BA1)
Gene Model and Products
Number of Transcripts
14
Number of Unique Polypeptides
11

Please see the GBrowse view of Dmel\Mical or the JBrowse view of Dmel\Mical for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Shares 5' UTR with nested gene (one isoform in each case).
Gene model reviewed during 5.49
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.46
Gene model reviewed during 5.42
Gene model reviewed during 6.02
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0082206
2806
810
FBtr0082207
10149
3002
FBtr0082210
15190
4723
FBtr0082213
15146
4723
FBtr0082208
9345
2734
FBtr0082209
15312
4723
FBtr0082212
15086
4723
FBtr0273185
2560
789
FBtr0306608
15264
4732
FBtr0306609
15345
4743
FBtr0347211
15585
3029
FBtr0347212
15030
4700
FBtr0347213
15210
4689
FBtr0347214
10116
2991
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0081684
91.4
810
9.10
FBpp0081685
332.5
3002
6.41
FBpp0081688
525.0
4723
7.09
FBpp0081691
525.0
4723
7.09
FBpp0081686
301.5
2734
6.11
FBpp0081687
525.0
4723
7.09
FBpp0081690
525.0
4723
7.09
FBpp0271693
88.9
789
8.66
FBpp0297563
526.1
4732
7.04
FBpp0297564
527.3
4743
7.04
FBpp0312509
340.6
3029
8.90
FBpp0312510
522.5
4700
7.03
FBpp0312511
521.2
4689
7.03
FBpp0312512
331.3
2991
6.41
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

4723 aa isoforms: Mical-PC, Mical-PD, Mical-PF, Mical-PG
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)
Interacts with plexA.
(UniProt, Q86BA1)
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Mical using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (17 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
inferred from direct assay
(assigned by UniProt )
Terms Based on Predictions or Assertions (0 terms)
Biological Process (11 terms)
Terms Based on Experimental Evidence (11 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from direct assay
(assigned by UniProt )
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:Sox14; FB:FBgn0005612
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:Sox14; FB:FBgn0005612
inferred from direct assay
(assigned by UniProt )
inferred from mutant phenotype
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
traceable author statement
Cellular Component (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
colocalizes_with plasma membrane
inferred from direct assay
inferred from direct assay
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
non-traceable author statement
Expression Data
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism

Comment: maternally deposited

Additional Descriptive Data
In stage 9 embryos, Mical is expressed in most cells of the germ band. From stage 13 to 15, Mical is most notably expressed in somatic muscles and in the midgut primordia. In stage 15 embryos, Mical becomes detectable in neurons of the CNS.
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Mical protein is not homogenously distributed
in developing muscles but accumulates preferentially at the plasma membrane and at muscle attachment sites. It can be seen to outline the muscles and emphasizing the cleft between neighboring muscle fibers.
Marker for
 
Subcellular Localization
CV Term
Evidence
References
colocalizes_with plasma membrane
inferred from direct assay
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Mical in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 13-16
Alleles, Insertions, Transgenic Constructs and Phenotypes
Classical and Insertion Alleles ( 24 )
Transgenic Constructs ( 25 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Mical
Allele of Mical
Mutagen
Associated Transgenic Construct
Stocks
Transgenic constructs containing regulatory region of Mical
reporter construct
Name
Expression Data
Deletions and Duplications ( 3 )
Summary of Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (5)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
9 of 15
Yes
Yes
8 of 15
No
Yes
6 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (5)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
8 of 15
Yes
Yes
7 of 15
No
Yes
5 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
Rattus norvegicus (Norway rat) (6)
6 of 13
Yes
Yes
5 of 13
No
Yes
2 of 13
No
Yes
1 of 13
No
No
1 of 13
No
Yes
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (2)
4 of 12
Yes
Yes
3 of 12
No
Yes
Danio rerio (Zebrafish) (8)
7 of 15
Yes
Yes
7 of 15
Yes
Yes
5 of 15
No
Yes
5 of 15
No
Yes
5 of 15
No
Yes
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (4)
1 of 15
Yes
Yes
1 of 15
Yes
No
1 of 15
Yes
No
1 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (1)
1 of 9
Yes
No
Saccharomyces cerevisiae (Brewer's yeast) (0)
No orthologs reported.
Schizosaccharomyces pombe (Fission yeast) (0)
No orthologs reported.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG0919001K )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091500J0 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W002J )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X002B )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G003O )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Human Disease Model Data
FlyBase Human Disease Model Reports
    Alleles Reported to Model Human Disease (Disease Ontology)
    Download
    Models ( 0 )
    Allele
    Disease
    Evidence
    References
    Interactions ( 0 )
    Allele
    Disease
    Interaction
    References
    Comments ( 0 )
     
    Human Orthologs (via DIOPT v7.1)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Subunit Structure (UniProtKB)
    Interacts with plexA.
    (UniProt, Q86BA1 )
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    Pathways
    Gene Group - Pathway Membership (FlyBase)
    External Data
    Linkouts
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    3R
    Recombination map
    3-49
    Cytogenetic map
    Sequence location
    3R:10,001,554..10,042,895 [-]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    85F5-85F7
    Limits computationally determined from genome sequence between P{EP}CrcEP3006 and P{lacW}s2681
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    85F3-85F6
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Location
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (21)
    Genomic Clones (35)
    cDNA Clones (120)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of
    Source for identity of: MICAL CG11685 CG11687 CG18668 CG18667
    Source for database merge of
    Additional comments
    Source for identity of MICAL CG11685 CG11687 CG18668 CG18667 was sequence comparison ( date:020710 ).
    Other Comments
    Mical has a cell-autonomous role in dendrite pruning and is specifically required for dendrite severing of class I and IV dendritic arborization neurons, but is dispensable for neuronal apoptosis in other apoptotic dendritic arborization neurons during early metamorphosis.
    Mical is required for the organisation of myofilaments into sarcomeres.
    Origin and Etymology
    Discoverer
    Etymology
    Gene is named "milchstrasse" (German for "milky way") based on the resemblance of bouton accumulations (marked with a fluorescent protein) to star clusters or galaxies at the neuromuscular junctions of mutant larvae.
    Identification
    External Crossreferences and Linkouts ( 91 )
    Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    Linkouts
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center cDNA clones
    DPiM - Drosophila Protein interaction map
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    FlyMine - An integrated database for Drosophila genomics
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    KEGG Genes - Molecular building blocks of life in the genomic space.
    modMine - A data warehouse for the modENCODE project
    Synonyms and Secondary IDs (17)
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (82)