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Gene Dmel\Mical

General Information
Symbol	Dmel\Mical	Species	D. melanogaster
Name	Molecule interacting with CasL	Annotation symbol	CG33208
Feature type	protein_coding_gene	FlyBase ID	FBgn0053208
Gene Model Status	Current	Stock availability	19 publicly available
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	85F5-85F7	Sequence location	3R:5,827,276..5,868,617 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Molecule interacting with CasL is referred to in FlyBase by the symbol Dmel\Mical (CG33208, FBgn0053208). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: actin binding; NADPH:sulfur oxidoreductase activity. There is experimental evidence that it is involved in the biological process: axon guidance; dendrite morphogenesis; actin filament depolymerization; neuron remodeling; sulfur oxidation; sarcomere organization; NADPH oxidation. 44 alleles are reported. The phenotypes of these alleles are annotated with: organ system; organ system subdivision; multicellular structure; peripheral nervous system; organelle; cytoplasmic part; adult segment; sense organ; anatomical structure; intracellular non-membrane-bounded organelle. It has 12 annotated transcripts and 12 annotated polypeptides. Protein features are: Calponin homology domain; Domain of unknown function DUF3585; Zinc finger, LIM-type. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed during early pupal stages. Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at moderate levels in the following post-embryonic organs or tissues: adult brain, adult crop, larval fat body, adult ovary, adult testis. Gene sequence location is 3R:5827276..5868617.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 85F5-85F7   Limits computationally determined from genome sequence between P{EP}CrcEP3006 and P{lacW}s2681
Experimentally Determined Cytological Location
Cytogenetic map Notes References 85F3-85F6   (determined by in situ hybridisation)   (Terman et al., 2002)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Mical for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.42 Gene model reviewed during 5.46 Gene model reviewed during 5.49 Low-frequency RNA-Seq exon junction(s) not annotated. Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters. Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Mical-RA FBtr0082206  NM_176450   2806   810 Mical-RB FBtr0082207  NM_176444   10149   3002 Mical-RC FBtr0082210  NM_176445   15190   4723 Mical-RD FBtr0082213  NM_176446   15146   4723 Mical-RE FBtr0082208  NM_176443   9345   2734 Mical-RF FBtr0082209  NM_176447   15312   4723 Mical-RG FBtr0082212  NM_176448   15086   4723 Mical-RH FBtr0082211  NM_176449   15099   4723 Mical-RI FBtr0273185  NM_001144560   2682   789 Mical-RK FBtr0306608  NM_001260085   15264   4732 Mical-RL FBtr0306609  NM_001260086   15345   4743 Mical-RM FBtr0306610  NM_001260087   15589   3112
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Mical-PA   FBpp0081684   91.4   810   9.10     NP_788627   AAF54478 Mical-PB   FBpp0081685   332.5   3002   6.41     NP_788621   AAO41532 Mical-PC   FBpp0081688   525.0   4723   7.09     NP_788622   AAO41533 Mical-PD   FBpp0081691   525.0   4723   7.09     NP_788623   AAO41534 Mical-PE   FBpp0081686   301.5   2734   6.11     NP_788620   AAO41531 Mical-PF   FBpp0081687   525.0   4723   7.09     NP_788624   AAO41535 Mical-PG   FBpp0081690   525.0   4723   7.09     NP_788625   AAO41536 Mical-PH   FBpp0081689   525.0   4723   7.09     NP_788626   AAO41537 Mical-PI   FBpp0271693   88.9   789   8.66     NP_001138032   ACL83491 Mical-PK   FBpp0297563   526.1   4732   7.04     NP_001247014   AFH06332 Mical-PL   FBpp0297564   527.3   4743   7.04     NP_001247015   AFH06333 Mical-PM   FBpp0297565   349.5   3112   8.99     NP_001247016   AFH06334
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Calponin homology domain (IPR001715) Zinc finger, LIM-type (IPR001781) Domain of unknown function DUF3585 (IPR022735)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA567663   AA567932   AA801710   AE014297 AAF54478   AE014297 AAO41531   AE014297 AAO41532   AE014297 AAO41533   AE014297 AAO41534   AE014297 AAO41535   AE014297 AAO41536   AE014297 AAO41537   AE014297 ACL83491   AF520713 AAM55242   AF520714 AAM55243   AF520715 AAM55244   AE014297 AFH06332   AE014297 AFH06333   AE014297 AFH06334   AI107348   AI257046   AI257522   AI257581   AI258463   AI259234   AI294964   AI297803   AI404788   AY061016   AY118290 AAM48319   BE975949   BE976070   BE977237   BE977948   BF486156   BF487898   BF487900   BF488337   BF488519   BF489326   BF489573   BF489726   BF489810   BF489821   BF490787   BF491352   BF493863   BF493905   BF496072   BF496488   BF496702   BF497487   BF497598   BF498039   BF498228   BF498618   BF499164   BF499350   BF499603   BF499677   BF500057   BF500157   BF500283   BF501971   BF502107   BF502550   BF504440   BF504781   BF505234   BF505579   BF505820   BF506237   BF506364   BF506492   BF506594   BG631942   BG634946   BG635305   BH610056   BI352054   BI355092   BI364471   BI365301   BI369252   BI369555   BI607235   BI609563   BI613269   BI615539   BI617074   BI618896   BT122067   BT126017   BT126186   CA803387   CA803390   CA803433   CA803626   CA803784   CA803894   CA804786   CA806162   CA806726   CA806743   CA807555   CA807779   CK135857   CK657127   CK658647   CK659008   CK659774   CK659825   CK659901   CO185577   CO190486   CO264991   CO271998   CO297275   CO297288   CO300843   CO303112   CO303617   CO316807   CO319966   CO322731   CO328502   CO343148   CZ466744   CZ468403   CZ470378   CZ474891   CZ484505   CZ484506   EC069930   EC072185   EC075301   EC084484   EC087352   EC088474   EC195120   EC197782   EC198818   EC201891   EC206940   EC216424   EC223198   EC229596   EC246521   EC248881   EC249879   EC253968   EC259950   EL884353   EL884354   EL884468   EL884469   EL884595   EL884708
UniProtKB/Swiss-Prot	Q86BA1
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 13 -- 16   dorsal pharyngeal muscle   (Fisher et al., 2012) embryonic/larval midgut   (Fisher et al., 2012) embryonic/larval muscle system   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Wasbrough et al., 2010)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
plasma membrane inferred from direct assay (Beuchle et al., 2007) terminal bouton inferred from direct assay (Beuchle et al., 2007)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at moderate levels in the following post-embryonic organs or tissues: adult brain, adult crop, larval fat body, adult ovary, adult testis. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3    46 central nervous system, pupae P8    36 head, virgin 1-day female    23 head, virgin 4-day female    22 head, virgin 20-day female    15 head, mated 1-day female    24 head, mated 4-day female    18 head, mated 20-day female    14 head, mated 1-day male    34 head, mated 4-day male    28 head, mated 20-day male    27 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    18 digestive system, larvae L3 wandering    23 digestive system, 1-day adult    6 digestive system, 4-day adult    9 digestive system, 20-day adult    9 fat body, larvae L3 wandering    18 fat body, white prepupae    52 fat body, pupae P8    34 carcass, larvae L3 wandering    97 carcass, 1-day adult    25 carcass, 4-day adult    22 carcass, 20-day adult    17 ovary, virgin 4-day female    12 ovary, mated 4-day female    14 testis, mated 4-day male    26 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3  (46) central nervous system, pupae P8  (36) head, virgin 1-day female    23 head, virgin 4-day female    22 head, virgin 20-day female    15 head, mated 1-day female    24 head, mated 4-day female    18 head, mated 20-day female    14 head, mated 1-day male  (34) head, mated 4-day male  (28) head, mated 20-day male  (27) salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    18 digestive system, larvae L3 wandering    23 digestive system, 1-day adult    6 digestive system, 4-day adult    9 digestive system, 20-day adult    9 fat body, larvae L3 wandering    18 fat body, white prepupae  (52) fat body, pupae P8  (34) carcass, larvae L3 wandering  (97) carcass, 1-day adult    25 carcass, 4-day adult    22 carcass, 20-day adult    17 ovary, virgin 4-day female    12 ovary, mated 4-day female    14 testis, mated 4-day male  26 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3    46 central nervous system, pupae P8    36 head, virgin 1-day female    23 head, virgin 4-day female    22 head, virgin 20-day female    15 head, mated 1-day female    24 head, mated 4-day female    18 head, mated 20-day female    14 head, mated 1-day male    34 head, mated 4-day male    28 head, mated 20-day male    27 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    18 digestive system, larvae L3 wandering    23 digestive system, 1-day adult    6 digestive system, 4-day adult    9 digestive system, 20-day adult    9 fat body, larvae L3 wandering    18 fat body, white prepupae    52 fat body, pupae P8    34 carcass, larvae L3 wandering    97 carcass, 1-day adult    25 carcass, 4-day adult    22 carcass, 20-day adult    17 ovary, virgin 4-day female    12 ovary, mated 4-day female    14 testis, mated 4-day male    26 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3    46 central nervous system, pupae P8    36 head, virgin 1-day female    23 head, virgin 4-day female    22 head, virgin 20-day female    15 head, mated 1-day female    24 head, mated 4-day female    18 head, mated 20-day female    14 head, mated 1-day male    34 head, mated 4-day male    28 head, mated 20-day male    27 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    18 digestive system, larvae L3 wandering    23 digestive system, 1-day adult    6 digestive system, 4-day adult    9 digestive system, 20-day adult    9 fat body, larvae L3 wandering    18 fat body, white prepupae    52 fat body, pupae P8    34 carcass, larvae L3 wandering    97 carcass, 1-day adult    25 carcass, 4-day adult    22 carcass, 20-day adult    17 ovary, virgin 4-day female    12 ovary, mated 4-day female    14 testis, mated 4-day male    26 accessory gland, mated 4-day male    6 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3    46 central nervous system, pupae P8    36 head, virgin 1-day female    23 head, virgin 4-day female    22 head, virgin 20-day female    15 head, mated 1-day female    24 head, mated 4-day female    18 head, mated 20-day female    14 head, mated 1-day male    34 head, mated 4-day male    28 head, mated 20-day male    27 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    18 digestive system, larvae L3 wandering    23 digestive system, 1-day adult    6 digestive system, 4-day adult    9 digestive system, 20-day adult    9 fat body, larvae L3 wandering    18 fat body, white prepupae    52 fat body, pupae P8    34 carcass, larvae L3 wandering    97 carcass, 1-day adult    25 carcass, 4-day adult    22 carcass, 20-day adult    17 ovary, virgin 4-day female    12 ovary, mated 4-day female    14 testis, mated 4-day male    26 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3  (46) central nervous system, pupae P8  (36) head, virgin 1-day female    23 head, virgin 4-day female    22 head, virgin 20-day female    15 head, mated 1-day female    24 head, mated 4-day female    18 head, mated 20-day female    14 head, mated 1-day male  (34) head, mated 4-day male  28 head, mated 20-day male  27 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    18 digestive system, larvae L3 wandering    23 digestive system, 1-day adult    6 digestive system, 4-day adult    9 digestive system, 20-day adult    9 fat body, larvae L3 wandering    18 fat body, white prepupae  (52) fat body, pupae P8  (34) carcass, larvae L3 wandering  (97) carcass, 1-day adult    25 carcass, 4-day adult    22 carcass, 20-day adult    17 ovary, virgin 4-day female    12 ovary, mated 4-day female    14 testis, mated 4-day male  26 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3    46 central nervous system, pupae P8    36 head, virgin 1-day female    23 head, virgin 4-day female    22 head, virgin 20-day female    15 head, mated 1-day female    24 head, mated 4-day female    18 head, mated 20-day female    14 head, mated 1-day male    34 head, mated 4-day male    28 head, mated 20-day male    27 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    18 digestive system, larvae L3 wandering    23 digestive system, 1-day adult    6 digestive system, 4-day adult    9 digestive system, 20-day adult    9 fat body, larvae L3 wandering    18 fat body, white prepupae    52 fat body, pupae P8    34 carcass, larvae L3 wandering    97 carcass, 1-day adult    25 carcass, 4-day adult    22 carcass, 20-day adult    17 ovary, virgin 4-day female    12 ovary, mated 4-day female    14 testis, mated 4-day male    26 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3    46 central nervous system, pupae P8    36 head, virgin 1-day female    23 head, virgin 4-day female    22 head, virgin 20-day female    15 head, mated 1-day female    24 head, mated 4-day female    18 head, mated 20-day female    14 head, mated 1-day male    34 head, mated 4-day male    28 head, mated 20-day male    27 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    18 digestive system, larvae L3 wandering    23 digestive system, 1-day adult    6 digestive system, 4-day adult    9 digestive system, 20-day adult    9 fat body, larvae L3 wandering    18 fat body, white prepupae    52 fat body, pupae P8    34 carcass, larvae L3 wandering    97 carcass, 1-day adult    25 carcass, 4-day adult    22 carcass, 20-day adult    17 ovary, virgin 4-day female    12 ovary, mated 4-day female    14 testis, mated 4-day male    26 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed during early pupal stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    9 embryo 04-06hr    14 embryo 06-08hr    14 embryo 08-10hr    16 embryo 10-12hr    12 embryo 12-14hr    22 embryo 14-16hr    19 embryo 16-18hr    15 embryo 18-20hr    16 embryo 20-22hr    10 embryo 22-24hr    11 larva L1    10 larva L2    8 larva L3 12hr old    7 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    19 larva L3 puffstage 7-9    48 white prepupae new    94 white prepupae 12hr    181 white prepupae 24hr    133 pupae 2d postWPP    107 pupae 3d postWPP    82 pupae 4d postWPP    31 adult male 01day    22 adult male 05day    20 adult male 30day    20 adult female 01day    15 adult female 05day    11 adult female 30day    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    9 embryo 04-06hr    14 embryo 06-08hr    14 embryo 08-10hr    16 embryo 10-12hr    12 embryo 12-14hr    22 embryo 14-16hr    19 embryo 16-18hr    15 embryo 18-20hr    16 embryo 20-22hr    10 embryo 22-24hr    11 larva L1    10 larva L2    8 larva L3 12hr old    7 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    19 larva L3 puffstage 7-9  (48) white prepupae new  (94) white prepupae 12hr  (181) white prepupae 24hr  (133) pupae 2d postWPP  (107) pupae 3d postWPP  (82) pupae 4d postWPP  (31) adult male 01day    22 adult male 05day    20 adult male 30day    20 adult female 01day    15 adult female 05day    11 adult female 30day    12 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    9 embryo 04-06hr    14 embryo 06-08hr    14 embryo 08-10hr    16 embryo 10-12hr    12 embryo 12-14hr    22 embryo 14-16hr    19 embryo 16-18hr    15 embryo 18-20hr    16 embryo 20-22hr    10 embryo 22-24hr    11 larva L1    10 larva L2    8 larva L3 12hr old    7 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    19 larva L3 puffstage 7-9    48 white prepupae new    94 white prepupae 12hr  (181) white prepupae 24hr  (133) pupae 2d postWPP  107 pupae 3d postWPP    82 pupae 4d postWPP    31 adult male 01day    22 adult male 05day    20 adult male 30day    20 adult female 01day    15 adult female 05day    11 adult female 30day    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    9 embryo 04-06hr    14 embryo 06-08hr    14 embryo 08-10hr    16 embryo 10-12hr    12 embryo 12-14hr    22 embryo 14-16hr    19 embryo 16-18hr    15 embryo 18-20hr    16 embryo 20-22hr    10 embryo 22-24hr    11 larva L1    10 larva L2    8 larva L3 12hr old    7 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    19 larva L3 puffstage 7-9    48 white prepupae new    94 white prepupae 12hr    181 white prepupae 24hr    133 pupae 2d postWPP    107 pupae 3d postWPP    82 pupae 4d postWPP    31 adult male 01day    22 adult male 05day    20 adult male 30day    20 adult female 01day    15 adult female 05day    11 adult female 30day    12 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    9 embryo 04-06hr    14 embryo 06-08hr    14 embryo 08-10hr    16 embryo 10-12hr    12 embryo 12-14hr    22 embryo 14-16hr    19 embryo 16-18hr    15 embryo 18-20hr    16 embryo 20-22hr    10 embryo 22-24hr    11 larva L1    10 larva L2    8 larva L3 12hr old    7 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    19 larva L3 puffstage 7-9    48 white prepupae new    94 white prepupae 12hr    181 white prepupae 24hr    133 pupae 2d postWPP    107 pupae 3d postWPP    82 pupae 4d postWPP    31 adult male 01day    22 adult male 05day    20 adult male 30day    20 adult female 01day    15 adult female 05day    11 adult female 30day    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    9 embryo 04-06hr    14 embryo 06-08hr    14 embryo 08-10hr    16 embryo 10-12hr    12 embryo 12-14hr    22 embryo 14-16hr    19 embryo 16-18hr    15 embryo 18-20hr    16 embryo 20-22hr    10 embryo 22-24hr    11 larva L1    10 larva L2    8 larva L3 12hr old    7 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    19 larva L3 puffstage 7-9  (48) white prepupae new  (94) white prepupae 12hr  (181) white prepupae 24hr  (133) pupae 2d postWPP  (107) pupae 3d postWPP  (82) pupae 4d postWPP  31 adult male 01day    22 adult male 05day    20 adult male 30day    20 adult female 01day    15 adult female 05day    11 adult female 30day    12 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    9 embryo 04-06hr    14 embryo 06-08hr    14 embryo 08-10hr    16 embryo 10-12hr    12 embryo 12-14hr    22 embryo 14-16hr    19 embryo 16-18hr    15 embryo 18-20hr    16 embryo 20-22hr    10 embryo 22-24hr    11 larva L1    10 larva L2    8 larva L3 12hr old    7 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    19 larva L3 puffstage 7-9    48 white prepupae new    94 white prepupae 12hr  (181) white prepupae 24hr  133 pupae 2d postWPP  107 pupae 3d postWPP    82 pupae 4d postWPP    31 adult male 01day    22 adult male 05day    20 adult male 30day    20 adult female 01day    15 adult female 05day    11 adult female 30day    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    9 embryo 04-06hr    14 embryo 06-08hr    14 embryo 08-10hr    16 embryo 10-12hr    12 embryo 12-14hr    22 embryo 14-16hr    19 embryo 16-18hr    15 embryo 18-20hr    16 embryo 20-22hr    10 embryo 22-24hr    11 larva L1    10 larva L2    8 larva L3 12hr old    7 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    19 larva L3 puffstage 7-9    48 white prepupae new    94 white prepupae 12hr    181 white prepupae 24hr    133 pupae 2d postWPP    107 pupae 3d postWPP    82 pupae 4d postWPP    31 adult male 01day    22 adult male 05day    20 adult male 30day    20 adult female 01day    15 adult female 05day    11 adult female 30day    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    11 Schneider line 2 Sg4    4 embryonic 1182-4H    11 embryonic GM2    18 embryonic Kc167    7 embryonic S1    6 embryonic S3    26 leg disc CME L1    6 wing disc CME-W2    6 wing disc ML-DmD8    8 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    13 wing disc ML-DmD21    2 wing disc ML-DmD32    11 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    2 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    10 ovary fGS/OSS    48 ovary OSC    37 ovary OSS    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    11 Schneider line 2 Sg4    4 embryonic 1182-4H    11 embryonic GM2    18 embryonic Kc167    7 embryonic S1    6 embryonic S3  26 leg disc CME L1    6 wing disc CME-W2    6 wing disc ML-DmD8    8 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    13 wing disc ML-DmD21    2 wing disc ML-DmD32    11 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    2 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    10 ovary fGS/OSS  (48) ovary OSC  (37) ovary OSS    24 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    11 Schneider line 2 Sg4    4 embryonic 1182-4H    11 embryonic GM2    18 embryonic Kc167    7 embryonic S1    6 embryonic S3    26 leg disc CME L1    6 wing disc CME-W2    6 wing disc ML-DmD8    8 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    13 wing disc ML-DmD21    2 wing disc ML-DmD32    11 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    2 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    10 ovary fGS/OSS    48 ovary OSC    37 ovary OSS    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    11 Schneider line 2 Sg4    4 embryonic 1182-4H    11 embryonic GM2    18 embryonic Kc167    7 embryonic S1    6 embryonic S3    26 leg disc CME L1    6 wing disc CME-W2    6 wing disc ML-DmD8    8 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    13 wing disc ML-DmD21    2 wing disc ML-DmD32    11 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    2 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    10 ovary fGS/OSS    48 ovary OSC    37 ovary OSS    24 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    11 Schneider line 2 Sg4    4 embryonic 1182-4H    11 embryonic GM2    18 embryonic Kc167    7 embryonic S1    6 embryonic S3    26 leg disc CME L1    6 wing disc CME-W2    6 wing disc ML-DmD8    8 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    13 wing disc ML-DmD21    2 wing disc ML-DmD32    11 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    2 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    10 ovary fGS/OSS    48 ovary OSC    37 ovary OSS    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    11 Schneider line 2 Sg4    4 embryonic 1182-4H    11 embryonic GM2    18 embryonic Kc167    7 embryonic S1    6 embryonic S3  26 leg disc CME L1    6 wing disc CME-W2    6 wing disc ML-DmD8    8 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    13 wing disc ML-DmD21    2 wing disc ML-DmD32    11 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    2 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    10 ovary fGS/OSS  (48) ovary OSC  (37) ovary OSS    24 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    11 Schneider line 2 Sg4    4 embryonic 1182-4H    11 embryonic GM2    18 embryonic Kc167    7 embryonic S1    6 embryonic S3    26 leg disc CME L1    6 wing disc CME-W2    6 wing disc ML-DmD8    8 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    13 wing disc ML-DmD21    2 wing disc ML-DmD32    11 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    2 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    10 ovary fGS/OSS    48 ovary OSC    37 ovary OSS    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    11 Schneider line 2 Sg4    4 embryonic 1182-4H    11 embryonic GM2    18 embryonic Kc167    7 embryonic S1    6 embryonic S3    26 leg disc CME L1    6 wing disc CME-W2    6 wing disc ML-DmD8    8 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    13 wing disc ML-DmD21    2 wing disc ML-DmD32    11 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    2 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    10 ovary fGS/OSS    48 ovary OSC    37 ovary OSS    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    21 cold shock, 4-day adult    19 heat shock, 4-day adult    10 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    17 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    20 Ethanol 2.5% 3 hrs, larvae L3    18 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    17 Caffeine 25 mg/ml 48 hrs, 4-day adult    15 Paraquat 5 mM 48 hrs, 4-day adult    17 Paraquat 10 mM 48 hrs, 4-day adult    25 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    21 cold shock, 4-day adult    19 heat shock, 4-day adult    10 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    17 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    20 Ethanol 2.5% 3 hrs, larvae L3    18 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    17 Caffeine 25 mg/ml 48 hrs, 4-day adult    15 Paraquat 5 mM 48 hrs, 4-day adult    17 Paraquat 10 mM 48 hrs, 4-day adult    25 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    21 cold shock, 4-day adult    19 heat shock, 4-day adult    10 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    17 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    20 Ethanol 2.5% 3 hrs, larvae L3    18 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    17 Caffeine 25 mg/ml 48 hrs, 4-day adult    15 Paraquat 5 mM 48 hrs, 4-day adult    17 Paraquat 10 mM 48 hrs, 4-day adult    25 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    21 cold shock, 4-day adult    19 heat shock, 4-day adult    10 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    17 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    20 Ethanol 2.5% 3 hrs, larvae L3    18 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    17 Caffeine 25 mg/ml 48 hrs, 4-day adult    15 Paraquat 5 mM 48 hrs, 4-day adult    17 Paraquat 10 mM 48 hrs, 4-day adult    25 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    21 cold shock, 4-day adult    19 heat shock, 4-day adult    10 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    17 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    20 Ethanol 2.5% 3 hrs, larvae L3    18 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    17 Caffeine 25 mg/ml 48 hrs, 4-day adult    15 Paraquat 5 mM 48 hrs, 4-day adult    17 Paraquat 10 mM 48 hrs, 4-day adult    25 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    21 cold shock, 4-day adult    19 heat shock, 4-day adult    10 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    17 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    20 Ethanol 2.5% 3 hrs, larvae L3    18 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    17 Caffeine 25 mg/ml 48 hrs, 4-day adult    15 Paraquat 5 mM 48 hrs, 4-day adult    17 Paraquat 10 mM 48 hrs, 4-day adult    25 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    21 cold shock, 4-day adult    19 heat shock, 4-day adult    10 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    17 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    20 Ethanol 2.5% 3 hrs, larvae L3    18 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    17 Caffeine 25 mg/ml 48 hrs, 4-day adult    15 Paraquat 5 mM 48 hrs, 4-day adult    17 Paraquat 10 mM 48 hrs, 4-day adult    25 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    21 cold shock, 4-day adult    19 heat shock, 4-day adult    10 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    17 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    20 Ethanol 2.5% 3 hrs, larvae L3    18 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    17 Caffeine 25 mg/ml 48 hrs, 4-day adult    15 Paraquat 5 mM 48 hrs, 4-day adult    17 Paraquat 10 mM 48 hrs, 4-day adult    25 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_25
External Data & Images
Linkouts	BDGP expression data • AT26738 HL03793 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0053208 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG33208-RB;CG33208-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Mical Stages(s) 1-3 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable Micalunspecified viable, with Scer\GAL4pnr-MD237 MicalGD7362 MicalGD9629 MicalGD9630 MicalGD13442 Sterility Allele fertile Micalunspecified Other Phenotypes Allele flight defective (with MicalG56) MicalI666 flight defective (with MicalI666) MicalG56 flightless Micalunspecified flightless, with Scer\GAL4Mef2.PR MicalGD9629 neuroanatomy defective Mical15256 MicalG56 MicalI696 MicalI1367 MicalK1496 Micalunspecified neuroanatomy defective, with Scer\GAL4221 MicalScer\UAS.fl neuroanatomy defective, with Scer\GAL4elav.PLu MicalCT.Scer\UAS.T:Myr-Src64B MicalGW.Scer\UAS MicalScer\UAS.cTa neuroanatomy defective, with Scer\GAL4ppk.1.9 MicalGD13442 MicalScer\UAS.fl neuroanatomy defective (with Df(3R)swp2MICAL) Mical15256 neuroanatomy defective (with MicalG56) MicalI666 neuroanatomy defective (with MicalI666) MicalG56 MicalK1496 neuroanatomy defective (with MicalK1496) MicalI666 neuroanatomy defective | embryonic stage (with Df(3R)swp2MICAL) MicalK583 neuroanatomy defective | first instar larval stage (with MicalI666) MicalK583 neuroanatomy defective | first instar larval stage (with MicalK583) MicalI666 neuroanatomy defective | larval stage MicalI666 MicalK583 neuroanatomy defective | larval stage (with MicalI666) MicalK1496 neuroanatomy defective | larval stage (with MicalK1496) MicalI666 neuroanatomy defective | third instar larval stage MicalI666 neuroanatomy defective | third instar larval stage (with Df(3R)swp2MICAL) MicalG158 MicalI666 MicalK583 MicalK1496 neuroanatomy defective | third instar larval stage (with MicalI666) MicalK583 neuroanatomy defective | third instar larval stage (with MicalK583) MicalI666 visible, with Scer\GAL4B11-98 MicalredoxCH.Scer\UAS.T:Zzzz\His6 MicalScer\UAS.s MicalΔCH.Scer\UAS MicalΔPIR.Scer\UAS MicalΔredox.Scer\UAS visible, with Scer\GAL4pnr-MD237 MicalGD7362 visible (with Df(3R)swp2MICAL) Micalc00824 Micalc03080 Micald06043 MicalEY09452 Micalf02010 MicalG56 MicalI666 MicalI696 MicalK583 MicalK584 MicalK1496 MicalKG00339 MicalKG06518 Phenotype manifest in Allele abdominal intersegmental nerve Micalunspecified abdominal segmental nerve Micalunspecified abdominal segmental nerve (with Df(3R)swp2MICAL) MicalK583 bouton MicalI666 bouton (with Df(3R)swp2MICAL) MicalG158 MicalI666 MicalK583 MicalK1496 bouton (with MicalG56) MicalI666 bouton (with MicalI666) MicalG56 MicalK583 MicalK1496 bouton (with MicalK583) MicalI666 bouton (with MicalK1496) MicalI666 dendrite Mical15256 dendrite, with Scer\GAL4221 MicalScer\UAS.fl dendrite, with Scer\GAL4ppk.1.9 MicalGD13442 MicalScer\UAS.fl dendrite (with Df(3R)swp2MICAL) Mical15256 dorsal multidendritic neuron ddaC Mical15256 dorsal multidendritic neuron ddaC, with Scer\GAL4221 MicalScer\UAS.fl dorsal multidendritic neuron ddaC, with Scer\GAL4ppk.1.9 MicalGD13442 MicalScer\UAS.fl dorsal multidendritic neuron ddaC (with Df(3R)swp2MICAL) Mical15256 embryonic/larval muscle system MicalI666 embryonic/larval muscle system (with Df(3R)swp2MICAL) MicalG158 MicalI666 MicalK583 MicalK1496 embryonic/larval neuromuscular junction MicalG56 MicalG158 MicalI666 MicalI696 MicalK583 MicalK1496 MicalI1367 embryonic/larval neuromuscular junction (with Df(3R)swp2MICAL) MicalG158 MicalI666 MicalK583 MicalK1496 embryonic/larval neuromuscular junction (with MicalG56) MicalI666 embryonic/larval neuromuscular junction (with MicalI666) MicalG56 MicalK583 MicalK1496 embryonic/larval neuromuscular junction (with MicalK583) MicalI666 embryonic/larval neuromuscular junction (with MicalK1496) MicalI666 embryonic/larval somatic muscle MicalK1496 embryonic/larval somatic muscle, with Scer\GAL4Mef2.PR MicalGD9629 embryonic/larval somatic muscle (with MicalG56) MicalI666 embryonic/larval somatic muscle (with MicalI666) MicalG56 MicalK583 embryonic/larval somatic muscle (with MicalK583) MicalI666 filamentous actin Micalunspecified filamentous actin, with Scer\GAL4B11-98 MicalScer\UAS.s MicalredoxCH.Scer\UAS.T:Zzzz\His6 filamentous actin (with MicalI666) MicalK583 filamentous actin (with MicalK583) MicalI666 flight muscle, with Scer\GAL4Mef2.PR MicalGD9629 flight muscle (with Df(3R)Exel6155) MicalK1496 growth cone, with Scer\GAL4eve.RN2 MicalredoxCH.Scer\UAS.T:Zzzz\His6 MicalΔPIR.Scer\UAS indirect flight muscle, with Scer\GAL4Mef2.PR MicalGD9629 indirect flight muscle (with Df(3R)swp2MICAL) MicalG158 intersegmental nerve, with Scer\GAL4elav.PLu MicalCT.Scer\UAS.T:Myr-Src64B MicalGW.Scer\UAS MicalScer\UAS.cTa macrochaeta, with Scer\GAL4B11-98 MicalScer\UAS.s MicalΔredox.Scer\UAS MicalΔCH.Scer\UAS MicalredoxCH.Scer\UAS.T:Zzzz\His6 MicalΔPIR.Scer\UAS macrochaeta (with Df(3R)swp2MICAL) MicalKG00339 Micalc00824 Micalc03080 Micald06043 Micalf02010 MicalG56 MicalI666 MicalI696 MicalK583 MicalK1496 MicalK584 MicalEY09452 MicalKG06518 myofibril (with Df(3R)swp2MICAL) MicalG158 presumptive embryonic/larval central nervous system Micalunspecified sarcomere MicalK1496 sarcomere, with Scer\GAL4Mef2.PR MicalGD9629 sarcomere (with MicalG56) MicalI666 sarcomere (with MicalI666) MicalG56 MicalK583 sarcomere (with MicalK583) MicalI666 segmental nerve, with Scer\GAL4elav.PLu MicalCT.Scer\UAS.T:Myr-Src64B MicalGW.Scer\UAS MicalScer\UAS.cTa trichogen cell, with Scer\GAL4pnr-MD237 MicalGD7362 ventral nerve cord Micalunspecified
Classical Alleles ( 22 )
For All Classical Alleles Show
Allele of Mical Class Mutagen Stocks Known lesion Micalc00824 piggyBac transposase 1 -- Micalc03080 piggyBac transposase 1 -- Micald06043 P-element activity 1 -- MicalEY09452 1 -- Micalf02010 piggyBac transposase 1 -- MicalGS15400 1 -- MicalKG00339 P-element activity 1 -- MicalKG06518 1 -- MicalMI01335 1 -- MicalMI03653 1 -- Mical15256 ethyl methanesulfonate 0 Yes Micalc05946 piggyBac transposase 0 -- MicalDG02306 0 -- MicalG158 ethyl methanesulfonate 0 Yes MicalG56 ethyl methanesulfonate 0 Yes MicalI1367 ethyl methanesulfonate 0 Yes MicalI666 ethyl methanesulfonate 0 Yes MicalI696 ethyl methanesulfonate 0 Yes MicalK1496 ethyl methanesulfonate 0 Yes MicalK583 ethyl methanesulfonate 0 Yes MicalK584 0 -- Micalunspecified 0 --
Alleles Carried on Transgenic Constructs ( 22 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Mical Class Mutagen Stocks Known lesion MicalGD13442 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes MicalGD9629 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes MicalGD9630 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes MicalGD7362 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes MicalJF01625 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes MicalKK102751 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes MicalCT.Scer\UAS.T:Myr-Src64B in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes MicalGW.Scer\UAS in vitro construct - amino acid replacement 0 Yes MicalN-ter.Scer\UAS in vitro construct - regulatory fusion 0 Yes MicalNIG.11685R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes MicalNIG.11687R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes MicalNIG.18667R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes MicalNIG.18668R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Micalredox.Scer\UAS.T:Zzzz\His6 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes MicalredoxCH.Scer\UAS.T:Zzzz\His6 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes MicalScer\UAS.cTa in vitro construct - regulatory fusion 0 Yes MicalScer\UAS.fl in vitro construct - regulatory fusion 0 Yes MicalScer\UAS.s in vitro construct - regulatory fusion 0 Yes MicalScer\UAS.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes MicalΔCH.Scer\UAS in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes MicalΔPIR.Scer\UAS in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes MicalΔredox.Scer\UAS in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)ED5438 (Kirilly et al., 2009) Df(3R)Exel6155 (Ryder, 2004.4.26, Kirilly et al., 2009) Df(3R)swp2MICAL (Ayoob et al., 2006, Beuchle et al., 2007, Kirilly et al., 2009, Terman et al., 2002)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{KK102751} NA P{NIG.11685R} NA P{NIG.11687R} NA P{NIG.18667R} NA P{NIG.18668R} NA P{TRiP.JF01625} NA P{UAS-EGFP-Mical} NA P{UAS-Mical.fl} NA P{UAS-Mical.N-ter} NA P{UAS-Mical.redox} NA P{UAS-Mical.redoxCH} NA P{UAS-Mical.s} NA P{UAS-Mical.ΔCH} NA P{UAS-Mical.ΔPIR} NA P{UAS-Mical.Δredox} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}MicalEY09452 NA P{GSV6}MicalGS15400 NA P{XP}Micald06043 NA miscellaneous insertions Mi{MIC}MicalMI01335 NA Mi{MIC}MicalMI03653 NA P{SUPor-P}MicalKG00339 NA P{SUPor-P}MicalKG06518 NA P{wHy}MicalDG02306 NA PBac{PB}Micalc00824 NA PBac{PB}Micalc03080 NA PBac{PB}Micalc05946 NA PBac{WH}Micalf02010 NA
Gene Ontology: Function, Process & Cellular Component ( 13 unique terms )
Terms Based on Experimental Evidence ( 11 terms )
Molecular Function
CV term Evidence References
actin binding inferred from direct assay (Hung et al., 2010) NADPH:sulfur oxidoreductase activity inferred from direct assay (Hung et al., 2011)
Biological Process
CV term Evidence References
actin filament depolymerization inferred from direct assay (Hung et al., 2010) inferred from mutant phenotype (Hung et al., 2011) axon guidance inferred from mutant phenotype (Terman et al., 2002, Terman and Kolodkin, 2004) dendrite morphogenesis inferred from genetic interaction with Sox14 (Kirilly et al., 2009) inferred from mutant phenotype (Kirilly et al., 2009) NADPH oxidation inferred from direct assay (Hung et al., 2011) neuron remodeling inferred from genetic interaction with Sox14 (Kirilly et al., 2009) inferred from mutant phenotype (Kirilly et al., 2009) sarcomere organization inferred from mutant phenotype (Beuchle et al., 2007) sulfur oxidation inferred from direct assay (Hung et al., 2011)
Cellular Component
CV term Evidence References
plasma membrane inferred from direct assay (Beuchle et al., 2007) terminal bouton inferred from direct assay (Beuchle et al., 2007)
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
actin binding inferred from sequence or structural similarity with UniProtKB:Q07931 (FlyBase, 1992-) zinc ion binding inferred from electronic annotation with InterPro:IPR001781 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
axon guidance traceable author statement (Castellani and Rougon, 2002)
Cellular Component
CV term Evidence References
cytosol non-traceable author statement (Castellani and Rougon, 2002)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Mical - HIP-R anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Mical allele Gene References
External Data
Linkouts	DPiM - DPiM, Drosophila Protein interaction Map • FBgn0053208 DroID - A comprehensive database of gene and protein interactions. • FBgn0053208 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41225
Orthologs
OrthoDB Orthologs (47) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6K6GPH Dipteran inclusive ortholog search EOG679FFZ Insect inclusive ortholog search EOG6280WC, EOG6PNWBQ Arthropod inclusive ortholog search EOG65QFV1, EOG6B5MKX Metazoa inclusive ortholog search EOG6MM17W
Orthologs in Drosophila Species (EOG6K6GPH)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Mical     Drosophila simulans   Dsim\GD20743     Drosophila simulans   Dsim\GD20744     Drosophila sechellia   Dsec\GM26195     Drosophila sechellia   Dsec\GM26194     Drosophila erecta   Dere\GG17310 Y   Drosophila yakuba   Dyak\GE24713 Y   Drosophila ananassae   Dana\GF17141 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA17370 Y   Drosophila persimilis   Dper\GL13150     Drosophila persimilis   Dper\GL17883     Drosophila persimilis   Dper\GL18281     Drosophila persimilis   Dper\GL24658     Drosophila persimilis   Dper\GL18764     Drosophila persimilis   Dper\GL26249     Drosophila persimilis   Dper\GL26031     Drosophila persimilis   Dper\GL20165     Drosophila persimilis   Dper\GL12215     Drosophila persimilis   Dper\GL23688     Drosophila persimilis   Dper\GL24957     Drosophila persimilis   Dper\GL16727     Drosophila persimilis   Dper\GL13276     Drosophila persimilis   Dper\GL27285 Y   Drosophila persimilis   Dper\GL16423     Drosophila persimilis   Dper\GL25841     Drosophila persimilis   Dper\GL18849     Drosophila willistoni   Dwil\GK11895 Y   Drosophila willistoni   Dwil\GK18065     Drosophila virilis   Dvir\GJ22902 Y   Drosophila mojavensis   Dmoj\GI13578     Drosophila mojavensis   Dmoj\GI22083     Drosophila mojavensis   Dmoj\GI22082     Drosophila grimshawi   Dgri\GH16326     Drosophila grimshawi   Dgri\GH21438     Drosophila grimshawi   Dgri\GH17953 Y   Drosophila grimshawi   Dgri\GH13270
Orthologs in non-Drosophila Dipterans (EOG679FFZ)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL005439   Aedes aegypti Yellow fever mosquito  Aaeg\AAEL006897   Aedes aegypti Yellow fever mosquito  Aaeg\AAEL005430   Anopheles gambiae Malaria mosquito  Agam\AGAP000222   Anopheles gambiae Malaria mosquito  Agam\AGAP003983   Anopheles gambiae Malaria mosquito  Agam\AGAP009473   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ013403   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ013401
Orthologs in non-Dipteran Insects (EOG6280WC, EOG6PNWBQ)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Bombyx mori Silkmoth  Bmor\BGIBMGA002106   Pediculus humanus Human body louse  Phum\PHUM131550
Orthologs in non-Insect Arthropods (EOG65QFV1, EOG6B5MKX)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Ixodes scapularis Deer tick  Isca\ISCW003395
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (8) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila erecta Dere\GG17310 Drosophila yakuba Dyak\GE24713 Drosophila ananassae Dana\GF17141 Drosophila pseudoobscura pseudoobscura Dpse\GA17370 Drosophila persimilis Dper\GL27285 Drosophila willistoni Dwil\GK11895 Drosophila virilis Dvir\GJ22902 Drosophila grimshawi Dgri\GH17953
Stocks & Reagents
Stocks Listed in FlyBase ( 19 )
Harvard	- P{XP}Micald06043 - PBac{PB}Micalc00824 - PBac{PB}Micalc03080 - PBac{WH}Micalf02010
VDRC	v105837 P{KK102751}VIE-260B v20672 w1118; P{GD9630}v20672 v20673 w1118; P{GD9630}v20673 v25371 w1118; P{GD9629}v25371 v25372 w1118; P{GD9629}v25372 v46096 w1118; P{GD13442}v46096 v46097 w1118; P{GD13442}v46097 v16267 w1118; P{GD7362}v16267/TM3
	More stocks available...
Genomic Clones ( 2 )
	BACR06O03 BACR39F04 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 131 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	AT26738 HL03793 LP14093 RH74552
Other clones	DPiM_AT26738 MIP19435
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT02232 AT02277 AT05801 AT06805 AT07418 AT07832 AT08208 AT08662 AT08812 AT08979 AT09121 AT09783 AT10420 AT10596 AT11665 AT11806 AT12393 AT12606 AT13059 AT13670 AT13890 AT14196 AT14305 AT14856 AT15067 AT15128 AT17569 AT17739 AT18216 AT20363 AT23004 AT23006 AT23578 AT23895 AT25295 AT25640 AT25861 AT25969 AT25982 AT27373 AT28074 AT31358 AT31839 GH04155 GH24621 GM03369 GM17371 GM29453 HL01602 HL02017 LP01669 LP02602 LP05295 LP05920 LP05986 LP08459 LP12188 LP15755 LP16407 LP17774 LP17880 LP18029 LP23171 RE49590 RE50564 RE55374 RE55770 RH14361 RH42384 RH45212 RH47288 RH49834
Other clones	5095 15189 31580 35392 58307 63178 74789 83116 83146 120128 162296 194726 214469.33 214545.39 bs46b05 bs46g08 bs61d04 bs71d02 CK01.35A.G10 CK01.39D.B10 CK01.59D.E2 CK01.78A.G6 EK010303 EK048349 EK105427 EK141143 EK179520 EK188746 EK216534 EK216562 EK240034 EK242234 EK265933 EK281605 EP09319 EP16203 ESG011a.E21_B05 ESG011d.E7.1_D07 ESG011d.E7.1_D11 ESG011d.E21_D07 ESG012b.TE_A02 ESG014d.E21_B07 ESG014d.E21_D05 ESG018c.E21.1_A08 ESG019c.E21_C10 ESG0110c.E21.1_C06 ESG0111a.B21.1_E12 ESG0115b.B21_A01 IP15662 IP15762 IP15862 IP15962 RH74254
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0053208 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41225
Antibody Information
Other Information
Discoverer
Etymology
	Gene is named "milchstrasse" (German for "milky way") based on the resemblance of bouton accumulations (marked with a fluorescent protein) to star clusters or galaxies at the neuromuscular junctions of mutant larvae. (Beuchle et al., 2007)
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: MICAL CG11685 CG11687 CG18668 CG18667 (FlyBase, 1996-)
Source for database merge of
Additional comments	Source for identity of MICAL CG11685 CG11687 CG18668 CG18667 was sequence comparison (date:020710). (FlyBase, 1996-)
Other Comments
	Mical has a cell-autonomous role in dendrite pruning and is specifically required for dendrite severing of class I and IV dendritic arborization neurons, but is dispensable for neuronal apoptosis in other apoptotic dendritic arborization neurons during early metamorphosis. (Kirilly et al., 2009) Mical is required for the organisation of myofilaments into sarcomeres. (Beuchle et al., 2007)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_176443 NM_176444 NM_176445 NM_176446 NM_176447 NM_176448 NM_176449 NM_176450 NM_001144560 NM_001260085 NM_001260086 NM_001260087 RefSeq (Proteins) • NP_788620 NP_788621 NP_788622 NP_788623 NP_788624 NP_788625 NP_788626 NP_788627 NP_001138032 NP_001247014 NP_001247015 NP_001247016 DDBJ / EMBL / GenBank • AAF54478 AAM48319 AAM55242 AAM55243 AAM55244 AAO41531 AAO41532 AAO41533 AAO41534 AAO41535 AAO41536 AAO41537 ACL83491 AF520713 AF520714 AF520715 AFH06332 AFH06333 AFH06334 AI259234 AY118290 BG631942 BH610056 CZ466744 CZ468403 CZ470378 CZ474891 CZ484505 CZ484506 Entrez Gene - A searchable database of RefSeq genes. • 41225 UniProtKB/Swiss-Prot • Q86BA1
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Calponin homology domain (IPR001715) Zinc finger, LIM-type (IPR001781) Domain of unknown function DUF3585 (IPR022735) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • AT26738 HL03793
Linkouts
	DPiM - DPiM, Drosophila Protein interaction Map • FBgn0053208 DroID - A comprehensive database of gene and protein interactions. • FBgn0053208 DRSC - Results from RNAi screens. • FBgn0053208 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0053208 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG33208-RB;CG33208-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0053208 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41225 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /genebrief/mical.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41225 modMine - Data generated by the modENCODE project. • FBgn0053208
Synonyms & Secondary IDs ( 15 )
Reported As
Symbol Synonym	CG11685   CG11687 (Yano et al., 2012) CG18484   CG18667   CG18668   CG33186   CG33190   CG33208 (Beuchle et al., 2007, Keleman et al., 2009.8.5, Ni et al., 2008) Mical (Hung et al., 2010, Yang and Terman, 2012, Langer et al., 2010, Toku et al., 2011, Kirilly et al., 2009, Jacques et al., 2009, Wasbrough et al., 2010, Hung et al., 2010, Hung et al., 2011) MICAL (Yazdani et al., 2008, Ayoob et al., 2004, Bakal et al., 2007, Ayoob et al., 2006, Magalhaes et al., 2007, Schnorrer et al., 2010, Tao and Rolls, 2011, Yano et al., 2012, Japanese National Institute of Genetics, 2012.5.21) mical (Beuchle et al., 2007, Kirilly et al., 2009) milch (Beuchle et al., 2007)
Name Synonym	mical (Beuchle et al., 2007) MICAL   milchstrasse (Beuchle et al., 2007) Molecule interacting with CasL   molecule that interacts with CasL (Ayoob et al., 2006)
Secondary FlyBase IDs
	FBgn0037774 FBgn0037775 FBgn0037776 FBgn0040538 FBgn0040539 FBgn0062839
References ( 51 )
Generate a list of	All references relating to this gene ( 51 )
List References by type	Research paper  ( 20 ) Supplementary material  ( 7 ) Review  ( 4 ) Personal communication to FlyBase  ( 11 ) Abstract  ( 1 ) FlyBase analysis  ( 3 ) Stock list  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 2 ) DNA/RNA sequence record  ( 1 )
Recent research papers ( 7 )
	Weber et al., 2012, PLoS ONE 7(4): e34745 Genome-Wide Association Analysis of Oxidative Stress Resistance in Drosophila melanogaster. [FBrf0218073] Yang and Terman, 2012, Neuron 74(1): 108--121 14-3-3ε couples protein kinase A to semaphorin signaling and silences plexin RasGAP-mediated axonal repulsion. [FBrf0218615] Yano et al., 2012, J. Neurogenet. 26(1): 53--63 Identification of proteasome components required for apical localization of chaoptin using functional genomics. [FBrf0217843] Hung et al., 2011, Science 334(6063): 1710--1713 Direct redox regulation of F-actin assembly and disassembly by Mical. [FBrf0217252] Kirilly et al., 2011, Neuron 72(1): 86--100 Intrinsic epigenetic factors cooperate with the steroid hormone ecdysone to govern dendrite pruning in Drosophila. [FBrf0216319] Tao and Rolls, 2011, J. Neurosci. 31(14): 5398--5405 Dendrites have a rapid program of injury-induced degeneration that is molecularly distinct from developmental pruning. [FBrf0213404] Toku et al., 2011, Comput. Biol. Chem. 35(5): 282--292 Reconstruction and crosstalk of protein-protein interaction networks of Wnt and Hedgehog signaling in Drosophila melanogaster. [FBrf0216393] Show all research papers ...
Recent reviews ( 1 )
	Broadie et al., 2011, Dev. Neurobiol. 71(11): 1102--1130 Extracellular matrix and its receptors in drosophila neural development. [FBrf0216377] Show all reviews ...