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Gene Dmel\CG33271

General Information
Symbol	Dmel\CG33271	Species	D. melanogaster
Name		Annotation symbol	CG33271
Feature type	protein_coding_gene	FlyBase ID	FBgn0053271
Gene Model Status	Current	Stock availability	1 publicly available
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	68C13-68C13	Sequence location	3L:11,570,106..11,570,595 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	This gene is referred to in FlyBase by the symbol Dmel\CG33271 (FBgn0053271). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. The biological processes in which it is involved are not known. 2 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of no expression detected. Peak expression observed within 18-24 hour embryonic stages, during early larval stages. Summary of FlyAtlas Anatomical Expression Data: No FlyAtlas data available because no Affy2 ProbeSet aligns to an exon of CG33271. Comments on Affy2 ProbeSet: No relevant information because no Affy2 ProbeSet aligns to an exon of CG33271. Gene sequence location is 3L:11570106..11570595.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 68C13-68C13   Limits computationally determined from genome sequence between P{PZ}l(3)0123901239&P{lacW}l(3)01239j9B4 and P{PZ}CycA02461&P{PZ}CycA03946
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\CG33271 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.50
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) CG33271-RA FBtr0076150  NM_206327   490   88
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein CG33271-PA   FBpp0075881   9.7   88   11.29     NP_996049   AAS65024
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014296 AAS65024   BT001271   BT133392   CO282627   CO314726   CO339434   EC088353   EC210952   EC258930
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q7KUG4
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of no expression detected. Peak expression observed within 18-24 hour embryonic stages, during early larval stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    1 embryo 10-12hr    6 embryo 12-14hr    9 embryo 14-16hr    12 embryo 16-18hr    40 embryo 18-20hr    90 embryo 20-22hr    197 embryo 22-24hr    189 larva L1    258 larva L2    73 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    1 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    1 embryo 10-12hr    6 embryo 12-14hr    9 embryo 14-16hr    12 embryo 16-18hr  (40) embryo 18-20hr  (90) embryo 20-22hr  (197) embryo 22-24hr  (189) larva L1  (258) larva L2  (73) larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    1 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    1 embryo 10-12hr    6 embryo 12-14hr    9 embryo 14-16hr    12 embryo 16-18hr    40 embryo 18-20hr    90 embryo 20-22hr  (197) embryo 22-24hr  (189) larva L1  (258) larva L2    73 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    1 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    1 embryo 10-12hr    6 embryo 12-14hr    9 embryo 14-16hr    12 embryo 16-18hr    40 embryo 18-20hr    90 embryo 20-22hr    197 embryo 22-24hr    189 larva L1    258 larva L2    73 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    1 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    1 embryo 10-12hr    6 embryo 12-14hr    9 embryo 14-16hr    12 embryo 16-18hr    40 embryo 18-20hr    90 embryo 20-22hr    197 embryo 22-24hr    189 larva L1    258 larva L2    73 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    1 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    1 embryo 10-12hr    6 embryo 12-14hr    9 embryo 14-16hr    12 embryo 16-18hr  (40) embryo 18-20hr  (90) embryo 20-22hr  (197) embryo 22-24hr  (189) larva L1  (258) larva L2  (73) larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    1 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    1 embryo 10-12hr    6 embryo 12-14hr    9 embryo 14-16hr    12 embryo 16-18hr    40 embryo 18-20hr    90 embryo 20-22hr  (197) embryo 22-24hr  (189) larva L1  (258) larva L2    73 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    1 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    1 embryo 10-12hr    6 embryo 12-14hr    9 embryo 14-16hr    12 embryo 16-18hr    40 embryo 18-20hr    90 embryo 20-22hr    197 embryo 22-24hr    189 larva L1    258 larva L2    73 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    1 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Treatments RNA-Seq
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_12 mE1_20_mRNA_expression_cluster_13
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0053271
Alleles & Phenotypes
Summary of Allele Phenotypes
Phenotype manifest in Allele
Classical Alleles ( 1 )
For All Classical Alleles Show
Allele of CG33271 Class Mutagen Stocks Known lesion CG33271MI06580 1 --
Alleles Carried on Transgenic Constructs ( 1 )
For All Alleles Carried on Transgenic Constructs Show
Allele of CG33271 Class Mutagen Stocks Known lesion CG33271KK116235 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{KK116235} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions Mi{MIC}CG33271MI06580 NA
Gene Ontology: Function, Process & Cellular Component ( 0 unique terms )
	There is no information supporting a biological process, cellular component or molecular function term for this gene. (Based on a review of available data before: 2006)
Terms Based on Experimental Evidence ( 0 terms )
Terms Based on Predictions or Assertions ( 0 terms )
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions CG33271 allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0053271
Orthologs
OrthoDB Orthologs (0) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search No orthologs identified Dipteran inclusive ortholog search No orthologs identified Insect inclusive ortholog search No orthologs identified Arthropod inclusive ortholog search No orthologs identified Metazoa inclusive ortholog search No orthologs identified
Orthologs in Drosophila Species (None identified)
No orthologies identified
Orthologs in non-Drosophila Dipterans (None identified)
No non-Drosophilid orthologies identified
Orthologs in non-Dipteran Insects (None identified)
No non-Dipteran orthologies identified
Orthologs in non-Insect Arthropods (None identified)
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (0) based on analysis using Dmel annotation version 4.3
No orthologs identified
Stocks & Reagents
Stocks Listed in FlyBase ( 1 )
Bloomington	41451 y1 w*; Mi{MIC}CG33271MI06580
Genomic Clones ( 1 )
	BACR03I05 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 8 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	AT04621
Other clones	FI19905
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones	45217 118144 214249.38 EK160850 EK237553 EP01958
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0053271 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 2768972
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: CG6100 CG14139 (Whitfield, 2000.12.15)
Additional comments	Annotation CG32084 split into CG33269, CG33270 and CG33271 in release 3.2 of the genome annotation. (FlyBase Genome Annotators, 2004) Annotation CG6100 split into CG32084 and CG32086 in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003)
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_206327 RefSeq (Proteins) • NP_996049 DDBJ / EMBL / GenBank • AAS65024 Entrez Gene - A searchable database of RefSeq genes. • 2768972 UniProtKB/TrEMBL • Q7KUG4
Other Crossreferences
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0053271 DRSC - Results from RNAi screens. • FBgn0053271 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0053271 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0053271 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 2768972 modMine - Data generated by the modENCODE project. • FBgn0053271
Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	CG6100 (Whitfield, 2000.12.15, ) CG14139 (Whitfield, 2000.12.15, ) CG32084 (Giot et al., 2003, ) CG33271 (Keleman et al., 2009.8.5)
Name Synonym
Secondary FlyBase IDs
	FBgn0036175 FBgn0040819 FBgn0052084
References ( 10 )
Supplementary material	Graveley et al., 2011, Nature 471(7339): Supplementary Table 33. Fuzzy Cluster Members. [FBrf0213980] The modENCODE Consortium, 2010, Science 330(6012): DataS14: Gene co-expression clusters. [FBrf0213979] Giot et al., 2003, Science 302(5651): Tables S1 to S7. [FBrf0166452]
Personal communication to FlyBase	Bellen, 2012.8.21, set1002.xml set1002.xml [FBrf0219255] Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510] Whitfield, 2000.12.15, Deleted protein_ids - part 1. Deleted protein_ids - part 1. [FBrf0132190]
FlyBase analysis	FlyBase Genome Annotators, 2004, Changes affecting gene model number or type in release 3.2 of the annotated D.melanogaster genome. Changes affecting gene model number or type in release 3.2 of the annotated D.melanogaster genome. [FBrf0166453] FlyBase Genome Annotators, 2002-2003, Changes affecting gene model number or type in releases 3.0 and 3.1 of the annotated D.melanogaster genome. Changes affecting gene model number or type in releases 3.0 and 3.1 of the annotated D.melanogaster genome. [FBrf0148886] FlyBase Curators, 2002-, Use of the ND evidence code for Gene Ontology (GO) terms in FlyBase. Use of the ND evidence code for Gene Ontology (GO) terms in FlyBase. [FBrf0159398]