FlyBase Gene Report: Dmel\ppk25

General Information

Symbol

Dmel\ppk25

Species

D. melanogaster

Name

pickpocket 25

Annotation Symbol

CG33349

Feature Type

protein_coding_gene

FlyBase ID

FBgn0053349

Gene Model Status

Current

Stock Availability

5 publicly available

Gene Summary

pickpocket 25 (ppk25) encodes a DEG/ENaC channel subunit involved in male courtship behavior and pheromone detection. [Date last reviewed: 2019-07-11] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

lounge lizard, llz

Key Links

Genomic Location

Cytogenetic map

42E1-42E1

Sequence location

2R:6,950,385..6,952,158 [+]

Recombination map

2-55

RefSeq locus

NT_033778 REGION:6950385..6952158

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (10 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables protein binding

inferred from physical interaction with FLYBASE:CheB42a; FB:FBgn0053348

(Ben-Shahar et al., 2010)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables ligand-gated sodium channel activity

inferred from biological aspect of ancestor with PANTHER:PTN000189658

(assigned by GO_Central )

(Gaudet et al., 2011)

enables sodium channel activity

inferred from sequence or structural similarity with MGI:MGI:101782

(FlyBase Curators, 2008-)

Biological Process (4 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

involved_in male courtship behavior

inferred from mutant phenotype

(Starostina et al., 2012, Lin et al., 2005)

involved_in response to pheromone

inferred from direct assay

(Lin et al., 2005)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in sodium ion transmembrane transport

inferred from biological aspect of ancestor with PANTHER:PTN000189658

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in sodium ion transport

inferred from sequence or structural similarity with MGI:MGI:101782

(FlyBase Curators, 2008-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

part_of protein-containing complex

inferred from physical interaction with FLYBASE:CheB42a; FB:FBgn0053348

(Ben-Shahar et al., 2010)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

located_in integral component of membrane

inferred from sequence or structural similarity with MGI:MGI:101782

(FlyBase Curators, 2008-)

is_active_in integral component of plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN000189658

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

PICKPOCKET GENES

Protein Family (UniProt)

Protein Signatures (InterPro)

Epithelial sodium channel

Summaries

Gene Snapshot

pickpocket 25 (ppk25) encodes a DEG/ENaC channel subunit involved in male courtship behavior and pheromone detection. [Date last reviewed: 2019-07-11]

Gene Group (FlyBase)

PICKPOCKET GENES -: The degenerin (DEG)/epithial Na⁺ channel (ENaC) gene family, known as the pickpocket genes in Drosophila, encodes subunits of non-voltage gated, amiloride-sensitive cation channels. Channels may be formed by homo- or heteromeric arrangements of subunits. Each subunit has two transmembrane domains and a large cysteine-rich extracellular loop domain. They are functionally diverse, with roles in fluid and salt absorbance, mechanosensation and chemosensation. (Adapted from FBrf0220901 and FBrf0159284).

Summary (Interactive Fly)

DEG/ENaC sodium channel subunit - required for activation of courtship behavior by chemosensory perception of female pheromones

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\ppk25 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry A1Z6S4)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Transposon inserted immediately 3' of gene.

Gene model reviewed during 5.46

May be component of a dicistronic gene (FBrf0191875); available data inconclusive.

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

ppk25-RA

FBtr0086169

NM_206044

1362

451

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

ppk25-PA

FBpp0085501

52.5

451

6.26

NP_995766

AAS64793

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Epithelial sodium channel

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

AE013599 / AAS64793

UniProt

A1Z6S4

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ppk25 using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

neuron

(Ben-Shahar et al., 2007)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | male

leg

(Ben-Shahar et al., 2007)

RACE

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | male

prothoracic leg

(Starostina et al., 2012)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | male

leg

(Ben-Shahar et al., 2007)

Additional Descriptive Data

ppk25 transcript is detected by RLM-RACE in male prothoracic legs, but not in adult heads from which the antennae had been removed.

(Starostina et al., 2012)

RT-PCR and northern blots show that ppk25 is enriched in male appendages relative to head and body and female parts. In situ to late embryos detects expression in two cells at the anterior end thought to be neurons. It is expressed in the same cells as CheB42a.

(Ben-Shahar et al., 2007)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of protein-containing complex

inferred from physical interaction with FLYBASE:CheB42a; FB:FBgn0053348

(Ben-Shahar et al., 2010)

Expression Deduced from Reporters

Reporter: P{ppk25-GAL4.S}

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | male

prothoracic leg

(Starostina et al., 2012)

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\ppk25 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\ppk25

View exonic expression by tissue for Dmel\ppk25

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

2768722/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0053349

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0053349

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0053349

Flygut - An atlas of the Drosophila adult midgut

FBgn0053349

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 4 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 8 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of ppk25

Transgenic constructs containing regulatory region of ppk25

Aberrations (Deficiencies and Duplications) ( 3 )

Inferred from experimentation ( 3 )

Gene disrupted in

Df(2R)Δ42E

(Lin et al., 2005)

Gene partially disrupted in

Df(2R)Δ5-2

(Lin et al., 2005)

Df(2R)Δ5-22

(Lin et al., 2005)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Dcr-2^UAS.cDa, Scer\GAL4^e22c, Scer\GAL4^sqh.PW

ppk25^KK109736

viable, with Dcr-2^UAS.cDa, Scer\GAL4^e22c, Scer\GAL4^sqh.PW

ppk25^GD1837

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

ppk25^GD1837

viable, with Scer\GAL4^Mef2.PR

ppk25^GD1837

viable, with Scer\GAL4^pnr-MD237

ppk25^GD1837

Other Phenotypes

Allele

abnormal courtship behavior

ppk25^Δ5-22

abnormal courtship behavior (with Df(2R)Δ5-22)

ppk25^e04217

abnormal courtship behavior (with Df(2R)Δ42E)

ppk25^e04217

abnormal courtship behavior | chemical conditional, with Scer\GAL4^elav.PU

ppk25^GD1837

abnormal courtship behavior | male

ppk25^unspecified

abnormal courtship behavior | male | heat sensitive, with Scer\GAL4^ppk25.PS

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (10)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\ASIC4

4 of 15

Yes

Hsap\ASIC5

4 of 15

Yes

3 of 15

3 of 15

3 of 15

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (8)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Asic5

4 of 15

Yes

3 of 15

3 of 15

3 of 15

3 of 15

2 of 15

2 of 15

2 of 15

Rattus norvegicus (Norway rat) (9)

Rnor\Asic5

4 of 13

Yes

3 of 13

3 of 13

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

Xenopus tropicalis (Western clawed frog) (11)

Xtro\asic1

3 of 12

Yes

Xtro\asic4

3 of 12

Yes

Xtro\asic5

3 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

Yes

Xtro\LOC101731449

1 of 12

Xtro\LOC101734161

1 of 12

Danio rerio (Zebrafish) (7)

Drer\asic1b

4 of 15

Yes

Drer\asic1c

4 of 15

Yes

Drer\asic4a

4 of 15

Yes

3 of 15

3 of 15

3 of 15

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (30)

Cele\acd-1

4 of 15

Yes

Cele\acd-2

4 of 15

Yes

Cele\acd-3

4 of 15

Yes

Cele\acd-4

4 of 15

Yes

Cele\acd-5

4 of 15

Yes

Cele\delm-1

4 of 15

Yes

Cele\delm-2

4 of 15

Yes

Cele\flr-1

4 of 15

Yes

Cele\del-1

3 of 15

Cele\del-9

3 of 15

Yes

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

Yes

2 of 15

2 of 15

2 of 15

2 of 15

Yes

Cele\egas-3

2 of 15

Yes

Cele\egas-4

2 of 15

Yes

2 of 15

2 of 15

2 of 15

1 of 15

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (31)

Gene Symbol

Score

Source

Alignment

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

ppk

3 of 10

rpk

3 of 10

2 of 10

2 of 10

2 of 10

2 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

ASIC4; acid sensing ion channel subunit family member 4

4 of 15

606715

ASIC5; acid sensing ion channel subunit family member 5

4 of 15

616693

ASIC1; acid sensing ion channel subunit 1

3 of 15

602866

ASIC2; acid sensing ion channel subunit 2

3 of 15

601784

ASIC3; acid sensing ion channel subunit 3

3 of 15

611741

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

ppk25 - CheB42a

anti tag coimmunoprecipitation, western blot

(Ben-Shahar et al., 2010)

ppk25 - ppk29

anti tag coimmunoprecipitation, anti tag western blot

(Liu et al., 2012)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

fru

suppressible

ppk25

(Zhang et al., 2020)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0053349

MIST (protein-protein) - An integrated Molecular Interaction Database

ppk25

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0053349

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-55

Cytogenetic map

42E1-42E1

Sequence location

2R:6,950,385..6,952,158 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

42E1-42E1

Limits computationally determined from genome sequence between P{lacW}ubl^k03203&P{PZ}CG3287⁰⁹⁹⁶⁷ and P{EP}EP2303&P{EP}EP2105

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-55

(FlyBase, 2016)

2-56.1

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (5)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

93027

w¹¹¹⁸; P{UAS-ppk25.S}3

27088

y¹ v¹; P{TRiP.JF02434}attP2

93028

w¹¹¹⁸; P{ppk25-GAL4.S}2; P{ppk25-GAL4.S}3

VDRC

v101808

P{KK109736}VIE-260B

v7343

w¹¹¹⁸; P{GD1837}v7343

Genomic Clones (23)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (0)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0053349

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

2768722

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: ppk25 CG33349

(Ben-Shahar, 2005.3.2)

Source for database merge of

Additional comments

Annotation CG15242 split into CG33348 (which corresponds to CheB42a) and CG33349 in release 3.2 of the genome annotation.

(FlyBase Genome Annotators, 2004)

Other Comments

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 25 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

2768722

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

A1Z6S4

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

A1Z6S4

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.