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Gene Dmel\Unc-89

General Information
Symbol	Dmel\Unc-89	Species	D. melanogaster
Name	Unc-89	Annotation symbol	CG33519
Feature type	protein_coding_gene	FlyBase ID	FBgn0053519
Gene Model Status	Current	Stock availability	8 publicly available
Also Known As	CG30171, BEST:HL01080
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	60B1-60B2	Sequence location	2R:19,883,066..19,901,384 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Unc-89 is referred to in FlyBase by the symbol Dmel\Unc-89 (CG33519, FBgn0053519). It is a protein_coding_gene from Drosophila melanogaster. An electronic pipeline based on InterPro domains suggests that it has the molecular function: Rho guanyl-nucleotide exchange factor activity; protein kinase activity; ATP binding. There is experimental evidence that it is involved in the biological process: adult somatic muscle development; sarcomere organization. 8 alleles are reported. The phenotypes of these alleles are annotated with: M band; indirect flight muscle. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Dbl homology (DH) domain; Fibronectin, type III; Immunoglobulin I-set; Immunoglobulin subtype; Immunoglobulin subtype 2; Immunoglobulin-like; Immunoglobulin-like fold; Myosin light chain kinase-related; Pleckstrin homology-type; Protein kinase, catalytic domain; Protein kinase-like domain; Serine/threonine-protein kinase, catalytic domain; Serine/threonine-protein kinase-like domain; Src homology-3 domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of extremely low expression. Peak expression observed during late pupal stages. Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at high levels in the following post-embryonic organs or tissues: adult crop, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, larval/adult hindgut, adult heart. Gene sequence location is 2R:19883066..19901384.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10	References Miller et al., 2008, Biophys. J. 95(5): 2391--2401
FB2012_01	Sequence features HFA04430 BKN50631 BKN50272 HFA04302 BKN25186 HFA04303 HFA04301 Controlled Vocabulary Terms protein kinase activity
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 60B1-60B2   Limits computationally determined from genome sequence between P{lacW}Phmk07623&P{lacW}tsrk05633 and P{EP}EP503
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Unc-89 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene merge based on protein alignment (BLASTX) data
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Unc-89-RB FBtr0091464  NM_001014545   12616   4158 Unc-89-RC FBtr0113464  NM_001103970   12796   4218
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Unc-89-PB   FBpp0099963   469.1   4158   5.78     NP_001014545   AAX52680 Unc-89-PC   FBpp0112375   475.7   4218   5.62     NP_001097440   ABV53900
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, catalytic domain (IPR000719) Immunoglobulin subtype (IPR003599) Fibronectin, type III (IPR003961) Immunoglobulin-like (IPR007110) Protein kinase-like domain (IPR011009) Dbl homology (DH) domain (IPR000219) Pleckstrin homology-type (IPR011993) Serine/threonine-protein kinase, catalytic domain (IPR002290) Immunoglobulin-like fold (IPR013783) Serine/threonine-protein kinase-like domain (IPR017442) Myosin light chain kinase-related (IPR020675) Src homology-3 domain (IPR001452) Immunoglobulin subtype 2 (IPR003598) Immunoglobulin I-set (IPR013098)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA567371   AA567462   AA698719   AE013599 AAX52680   AE013599 ABV53900   AI389348   AK268246   AW940785   AY051554 AAK92978   AY118318 AAM48347   BG637642   CO178787   CO282272   CO292037   CO304696   CO305608   CO316107   CO339860   CZ467491   DQ431841 ABD83643   EC051002   EC051023   EC051992   EC053028   EC053053   EC053531   EC054481   EC054491   EC055016   EC056191   EC062072   EC074988   EC086632   EC088822   EC195857   EC199938   EC200313   EC205172   EC225855   EC235506   EC237366   EC249240   EC259829   ES689250   ES689319   ES689347   ES689529   ES693751   ES693773   ES693807   ES693831   ES693915   ES694291   ES696126   EW712797   EW712899   GH291255   GH291265   GH291400   GH942086   GR705722   GR705860
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q1ZYJ8 Q7K4Z6 Q8MT81 A8DYP0 Q59E65
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Wasbrough et al., 2010) day 7 of adulthood -- day 49 of adulthood   adult heart   (Cammarato et al., 2011)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0053519    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0053519 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of extremely low expression. Peak expression observed during late pupal stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0053519 expression level Developmental Stage   Expression Level embryo 00-02hr    5 embryo 02-04hr    7 embryo 04-06hr    13 embryo 06-08hr    21 embryo 08-10hr    34 embryo 10-12hr    24 embryo 12-14hr    31 embryo 14-16hr    390 embryo 16-18hr    1698 embryo 18-20hr    4156 embryo 20-22hr    4598 embryo 22-24hr    4353 larva L1    2703 larva L2    2338 larva L3 12hr old    4699 larva L3 puffstage 1-2    1965 larva L3 puffstage 3-6    1004 larva L3 puffstage 7-9    241 white prepupae new    112 white prepupae 12hr    1030 white prepupae 24hr    233 pupae 2d postWPP    3909 pupae 3d postWPP    18223 pupae 4d postWPP    18166 adult male 01day    7929 adult male 05day    4318 adult male 30day    2934 adult female 01day    6335 adult female 05day    801 adult female 30day    630 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    5 embryo 02-04hr    7 embryo 04-06hr    13 embryo 06-08hr    21 embryo 08-10hr    34 embryo 10-12hr    24 embryo 12-14hr    31 embryo 14-16hr  (390) embryo 16-18hr  (1698) embryo 18-20hr  (4156) embryo 20-22hr  (4598) embryo 22-24hr  (4353) larva L1  (2703) larva L2  (2338) larva L3 12hr old  (4699) larva L3 puffstage 1-2  (1965) larva L3 puffstage 3-6  (1004) larva L3 puffstage 7-9  (241) white prepupae new  (112) white prepupae 12hr  (1030) white prepupae 24hr  (233) pupae 2d postWPP  (3909) pupae 3d postWPP  (18223) pupae 4d postWPP  (18166) adult male 01day  (7929) adult male 05day  (4318) adult male 30day  (2934) adult female 01day  (6335) adult female 05day  (801) adult female 30day  (630) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    5 embryo 02-04hr    7 embryo 04-06hr    13 embryo 06-08hr    21 embryo 08-10hr    34 embryo 10-12hr    24 embryo 12-14hr    31 embryo 14-16hr    390 embryo 16-18hr  (1698) embryo 18-20hr  (4156) embryo 20-22hr  (4598) embryo 22-24hr  (4353) larva L1  (2703) larva L2  (2338) larva L3 12hr old  (4699) larva L3 puffstage 1-2  (1965) larva L3 puffstage 3-6    1004 larva L3 puffstage 7-9    241 white prepupae new    112 white prepupae 12hr    1030 white prepupae 24hr    233 pupae 2d postWPP  (3909) pupae 3d postWPP  (18223) pupae 4d postWPP  (18166) adult male 01day  (7929) adult male 05day  (4318) adult male 30day  (2934) adult female 01day  (6335) adult female 05day    801 adult female 30day    630 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    5 embryo 02-04hr    7 embryo 04-06hr    13 embryo 06-08hr    21 embryo 08-10hr    34 embryo 10-12hr    24 embryo 12-14hr    31 embryo 14-16hr    390 embryo 16-18hr    1698 embryo 18-20hr    4156 embryo 20-22hr    4598 embryo 22-24hr    4353 larva L1    2703 larva L2    2338 larva L3 12hr old    4699 larva L3 puffstage 1-2    1965 larva L3 puffstage 3-6    1004 larva L3 puffstage 7-9    241 white prepupae new    112 white prepupae 12hr    1030 white prepupae 24hr    233 pupae 2d postWPP    3909 pupae 3d postWPP  (18223) pupae 4d postWPP  (18166) adult male 01day    7929 adult male 05day    4318 adult male 30day    2934 adult female 01day    6335 adult female 05day    801 adult female 30day    630 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    5 embryo 02-04hr    7 embryo 04-06hr    13 embryo 06-08hr    21 embryo 08-10hr    34 embryo 10-12hr    24 embryo 12-14hr    31 embryo 14-16hr    390 embryo 16-18hr    1698 embryo 18-20hr    4156 embryo 20-22hr    4598 embryo 22-24hr    4353 larva L1    2703 larva L2    2338 larva L3 12hr old    4699 larva L3 puffstage 1-2    1965 larva L3 puffstage 3-6    1004 larva L3 puffstage 7-9    241 white prepupae new    112 white prepupae 12hr    1030 white prepupae 24hr    233 pupae 2d postWPP    3909 pupae 3d postWPP    18223 pupae 4d postWPP    18166 adult male 01day    7929 adult male 05day    4318 adult male 30day    2934 adult female 01day    6335 adult female 05day    801 adult female 30day    630 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0053519 expression level Developmental Stage   Expression Level embryo 00-02hr    5 embryo 02-04hr    7 embryo 04-06hr    13 embryo 06-08hr    21 embryo 08-10hr    34 embryo 10-12hr    24 embryo 12-14hr    31 embryo 14-16hr    390 embryo 16-18hr    1698 embryo 18-20hr    4156 embryo 20-22hr    4598 embryo 22-24hr    4353 larva L1    2703 larva L2    2338 larva L3 12hr old    4699 larva L3 puffstage 1-2    1965 larva L3 puffstage 3-6    1004 larva L3 puffstage 7-9    241 white prepupae new    112 white prepupae 12hr    1030 white prepupae 24hr    233 pupae 2d postWPP    3909 pupae 3d postWPP    18223 pupae 4d postWPP    18166 adult male 01day    7929 adult male 05day    4318 adult male 30day    2934 adult female 01day    6335 adult female 05day    801 adult female 30day    630 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    5 embryo 02-04hr    7 embryo 04-06hr    13 embryo 06-08hr    21 embryo 08-10hr    34 embryo 10-12hr    24 embryo 12-14hr    31 embryo 14-16hr  (390) embryo 16-18hr  (1698) embryo 18-20hr  (4156) embryo 20-22hr  (4598) embryo 22-24hr  (4353) larva L1  (2703) larva L2  (2338) larva L3 12hr old  (4699) larva L3 puffstage 1-2  (1965) larva L3 puffstage 3-6  (1004) larva L3 puffstage 7-9  (241) white prepupae new  112 white prepupae 12hr  (1030) white prepupae 24hr  (233) pupae 2d postWPP  (3909) pupae 3d postWPP  (18223) pupae 4d postWPP  (18166) adult male 01day  (7929) adult male 05day  (4318) adult male 30day  (2934) adult female 01day  (6335) adult female 05day  (801) adult female 30day  (630) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    5 embryo 02-04hr    7 embryo 04-06hr    13 embryo 06-08hr    21 embryo 08-10hr    34 embryo 10-12hr    24 embryo 12-14hr    31 embryo 14-16hr    390 embryo 16-18hr  1698 embryo 18-20hr  (4156) embryo 20-22hr  (4598) embryo 22-24hr  (4353) larva L1  (2703) larva L2  2338 larva L3 12hr old  (4699) larva L3 puffstage 1-2  1965 larva L3 puffstage 3-6    1004 larva L3 puffstage 7-9    241 white prepupae new    112 white prepupae 12hr    1030 white prepupae 24hr    233 pupae 2d postWPP  (3909) pupae 3d postWPP  (18223) pupae 4d postWPP  (18166) adult male 01day  (7929) adult male 05day  (4318) adult male 30day  (2934) adult female 01day  (6335) adult female 05day    801 adult female 30day    630 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    5 embryo 02-04hr    7 embryo 04-06hr    13 embryo 06-08hr    21 embryo 08-10hr    34 embryo 10-12hr    24 embryo 12-14hr    31 embryo 14-16hr    390 embryo 16-18hr    1698 embryo 18-20hr    4156 embryo 20-22hr    4598 embryo 22-24hr    4353 larva L1    2703 larva L2    2338 larva L3 12hr old    4699 larva L3 puffstage 1-2    1965 larva L3 puffstage 3-6    1004 larva L3 puffstage 7-9    241 white prepupae new    112 white prepupae 12hr    1030 white prepupae 24hr    233 pupae 2d postWPP    3909 pupae 3d postWPP  18223 pupae 4d postWPP  18166 adult male 01day    7929 adult male 05day    4318 adult male 30day    2934 adult female 01day    6335 adult female 05day    801 adult female 30day    630 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    5 embryo 02-04hr    7 embryo 04-06hr    13 embryo 06-08hr    21 embryo 08-10hr    34 embryo 10-12hr    24 embryo 12-14hr    31 embryo 14-16hr    390 embryo 16-18hr    1698 embryo 18-20hr    4156 embryo 20-22hr    4598 embryo 22-24hr    4353 larva L1    2703 larva L2    2338 larva L3 12hr old    4699 larva L3 puffstage 1-2    1965 larva L3 puffstage 3-6    1004 larva L3 puffstage 7-9    241 white prepupae new    112 white prepupae 12hr    1030 white prepupae 24hr    233 pupae 2d postWPP    3909 pupae 3d postWPP    18223 pupae 4d postWPP    18166 adult male 01day    7929 adult male 05day    4318 adult male 30day    2934 adult female 01day    6335 adult female 05day    801 adult female 30day    630 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0053519    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0053519 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at high levels in the following post-embryonic organs or tissues: adult crop, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, larval/adult hindgut, adult heart. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut no informative data Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea no informative data Larval Carcass no informative data Adult Head no informative data Adult Eye no informative data Adult Brain no informative data Adult Thoracic-Abdominal Ganglion     Adult Crop no informative data Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules     Adult Fat Body no informative data Adult Salivary Gland     Adult Heart no informative data Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary     Adult Testis no informative data Adult Male Accessory Gland no informative data Adult Carcass no informative data modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_15
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0053519 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG30171-RB;CG30175-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable, with Scer\GAL4pnr-MD237 Unc-89GD10014 Unc-89GD14839 Phenotype manifest in Allele indirect flight muscle, with Scer\GAL4Mef2.PR Unc-89GD14839 M band, with Scer\GAL4Mef2.PR Unc-89GD14839
Classical Alleles ( 4 )
For All Classical Alleles Show
Allele of Unc-89 Class Mutagen Stocks Known lesion Unc-89c01844 piggyBac transposase 1 -- Unc-89EY15484 1 -- Unc-89MB00669 1 -- Unc-89MI02198 1 --
Alleles Carried on Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Unc-89 Class Mutagen Stocks Known lesion Unc-89GD14839 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Unc-89HMS00963 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Unc-89KK104226 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Unc-89GD10014 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)Exel6080 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD10014} NA P{GD14839} NA P{KK104226} NA P{TRiP.HMS00963} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions Mi{MIC}Unc-89MI02198 NA PBac{PB}Unc-89c01844 NA insertion of mobile activating element P{EPgy2}Unc-89EY15484 NA insertion of enhancer trap binary system Mi{ET1}Unc-89MB00669 No
Gene Ontology: Function, Process & Cellular Component ( 8 unique terms )
Terms Based on Experimental Evidence ( 2 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
adult somatic muscle development inferred from mutant phenotype (Schnorrer et al., 2010) sarcomere organization inferred from mutant phenotype (Schnorrer et al., 2010)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 6 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002290 (FlyBase Curators et al., 2004-) protein kinase activity inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002290 (FlyBase Curators et al., 2004-) Rho guanyl-nucleotide exchange factor activity inferred from electronic annotation with InterPro:IPR000219 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
protein phosphorylation inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002290 (FlyBase Curators et al., 2004-) regulation of Rho protein signal transduction inferred from electronic annotation with InterPro:IPR000219 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
intracellular inferred from electronic annotation with InterPro:IPR000219 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Unc-89 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 77919 DroID - A comprehensive database of gene and protein interactions. • FBgn0053519 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 3346201
Orthologs
Genome-wide drosophilid orthologs	Dana\GF12043 Dere\GG19967 Dgri\GH21704 Dmoj\GI20693 Dper\GL10435 Dpse\GA24366 Dsec\GM15480 Dvir\GJ19688 Dwil\GK21935 Dyak\GE11499
Curated drosophilid orthologs
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL013559-PA Aedes aegypti (mosquito) AGAP005471-PA Anopheles gambiae (mosquito) XP_392361.3 Apis mellifera (honey bee) XP_001942691.1 Acyrthosiphon pisum (pea aphid) 74213 Branchiostoma floridae (lancelet) BGIBMGA013531-PA Bombyx mori (silk worm) ENSBTAP00000020278 Bos taurus (bovine) CN17017 Caenorhabditis brenneri (small nematode) CBP02940 Caenorhabditis briggsae (nematode) CE34251 Caenorhabditis elegans (worm) ENSCAFP00000022838 Canis familiaris (dog) JA09251 Caenorhabditis japonica (nematode) ENSCPOP00000002241 Cavia porcellus (guinea pig) RP45264 Caenorhabditis remanei (nematode) 315830 Daphnia pulex (daphnia) ENSDARP00000059571 Danio rerio (zebra fish) ENSECAP00000014761 Equus caballus (horse) ENSGACP00000018885 Gymnopilus aculeatus (mushroom) ENSGALP00000014775 Gallus gallus (chicken) 164811 Hypsipyla robusta (cedar shoot borer) ENSP00000311684 Homo sapiens (human) ISCW023694-PA Ixodes scapularis (deer tick) ENSMMUP00000023577 Macaca mulatta (rhesus monkey) ENSMUSP00000084361 Mus musculus (house mouse) XP_001602095.1 Nasonia vitripennis (wasp) ENSOANP00000013774 Ornithorynchus anatinus (platypus) ENSORLP00000006915 Oryzias latipes (japanese killifish) PHUM000716-PA Pediculus humanus (human body louse) PP27267 Pristionchus pacificus (nematode) ENSPTRP00000022133 Pan troglodytes (chimpanzee) ENSRNOP00000026941 Rattus norvegicus (norway rat) XP_787033.2 Strongylocentrotus purpuratus (purple sea urchin) XP_966368.2 Tribolium castaneum (red flour beetle) ENSTNIP00000011176 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000008607 Takifugu rubripes (pufferfish Takifugu) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV50066 NV11575 LH11800 LH11798 LH11794 ISCW023694 FBgn0229302 FBgn0223920 FBgn0206831 FBgn0206830 FBgn0196297 FBgn0196296 FBgn0196295 FBgn0170398 JGI_V11_315830 FBgn0245763 FBgn0148045 FBgn0143428 FBgn0129165 FBgn0112167 FBgn0089082 BGIBMGA013531 ACYPI008736 GB10576 AGAP005471 AAEL013559 PB24558 PB24563 PB27086 PHUM405120 PHUM405130 GLEAN_02779
Stocks & Reagents
Stocks Listed in FlyBase ( 8 )
Bloomington	34292 y1 w*; Mi{MIC}Unc-89MI02198 22866 y1 w67c23; Mi{ET1}Unc-89MB00669 21139 y1 w67c23; P{EPgy2}Unc-89EY15484 34000 y1 sc* v1; P{TRiP.HMS00963}attP2
Harvard	- PBac{PB}Unc-89c01844
VDRC	v106267 P{KK104226}VIE-260B v29412 w1118; P{GD14839}v29412 v29413 w1118; P{GD14839}v29413/TM3
Genomic Clones ( 1 )
	BACR28M05 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 37 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH20492 HL01080
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	HL01302 HL05552
Other clones	44565 53815 59129 70478 71021 72693 109018 117887 145788 146120 214417.79 273622 CK01.72D.C12 DMG5-chr2R.34.012.pred.a EC051002 EC051023 EC051992 EC053028 EC053053 EC053531 EC054481 EC054491 EC055016 EC056191 EK057544 EK160126 EK199041 EK245807 EK249064 EK263652 EP09529 MB6-CG33519.a RP004260017
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0053519 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37829 246499 3346201
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Unc-89 CG33519 (Russell and Geisler, 2006.3.3)
Source for database merge of	Source for merge of: BEST:HL01080 CG30171 (FlyBase, 1996-) Source for merge of: CG18019 CG3901 (FlyBase Genome Annotators, 2002-2003) Source for merge of: CG18019 CG18020 CG18021 (FlyBase Genome Annotators, 2005, FlyBase, 1996-) Source for merge of: CG18019 CG18020 CG18021 CG3901 (BDGP Project Members, 2000-) Source for merge of: CG18020 CG18021 (Misra, 2001.8.10)
Additional comments	Source for merge of CG18019 CG18020 CG18021 was a shared cDNA (date:010720). (FlyBase Genome Annotators, 2005, FlyBase, 1996-) Annotations CG18019, CG18020, CG18021 and CG3901 merged as CG30171 in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003) Source for merge of BEST:HL01080 CG30171 was a shared cDNA (date:030206). (FlyBase, 1996-)
Other Comments
	dsRNA made from templates generated with primers directed against CG30171 tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) New annotation (CG30175) in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_001014545 NM_001103970 RefSeq (Proteins) • NP_001014545 NP_001097440 DDBJ / EMBL / GenBank • AA567371 AAF47133.2 AAF47134.2 AAK92978 AAM48347 AAM68288.1 AAX52680 ABD83643 ABV53900 AI389348 AW940785 AY051554 AY118318 BG637642 CZ467491 DQ431841 Entrez Gene - A searchable database of RefSeq genes. • 3346201 UniProtKB/TrEMBL • A8DYP0 Q1ZYJ8 Q7K4Z6 Q8MT81 Q59E65
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, catalytic domain (IPR000719) Immunoglobulin subtype (IPR003599) Fibronectin, type III (IPR003961) Immunoglobulin-like (IPR007110) Protein kinase-like domain (IPR011009) Dbl homology (DH) domain (IPR000219) Pleckstrin homology-type (IPR011993) Serine/threonine-protein kinase, catalytic domain (IPR002290) Immunoglobulin-like fold (IPR013783) Serine/threonine-protein kinase-like domain (IPR017442) Myosin light chain kinase-related (IPR020675) Src homology-3 domain (IPR001452) Immunoglobulin subtype 2 (IPR003598) Immunoglobulin I-set (IPR013098)
Linkouts
	BioGRID - A database of protein and genetic interactions • 77919 DroID - A comprehensive database of gene and protein interactions. • FBgn0053519 DRSC - Results from RNAi screens. • FBgn0053519 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0053519 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG30171-RB;CG30175-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0053519 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37829 246499 3346201 InParanoid A subset of ortholog calls from InParanoid. • AAEL013559-PA Aedes aegypti (mosquito) AGAP005471-PA Anopheles gambiae (mosquito) XP_392361.3 Apis mellifera (honey bee) XP_001942691.1 Acyrthosiphon pisum (pea aphid) 74213 Branchiostoma floridae (lancelet) BGIBMGA013531-PA Bombyx mori (silk worm) ENSBTAP00000020278 Bos taurus (bovine) CN17017 Caenorhabditis brenneri (small nematode) CBP02940 Caenorhabditis briggsae (nematode) CE34251 Caenorhabditis elegans (worm) ENSCAFP00000022838 Canis familiaris (dog) JA09251 Caenorhabditis japonica (nematode) ENSCPOP00000002241 Cavia porcellus (guinea pig) RP45264 Caenorhabditis remanei (nematode) 315830 Daphnia pulex (daphnia) ENSDARP00000059571 Danio rerio (zebra fish) ENSECAP00000014761 Equus caballus (horse) ENSGACP00000018885 Gymnopilus aculeatus (mushroom) ENSGALP00000014775 Gallus gallus (chicken) 164811 Hypsipyla robusta (cedar shoot borer) ENSP00000311684 Homo sapiens (human) ISCW023694-PA Ixodes scapularis (deer tick) ENSMMUP00000023577 Macaca mulatta (rhesus monkey) ENSMUSP00000084361 Mus musculus (house mouse) XP_001602095.1 Nasonia vitripennis (wasp) ENSOANP00000013774 Ornithorynchus anatinus (platypus) ENSORLP00000006915 Oryzias latipes (japanese killifish) PHUM000716-PA Pediculus humanus (human body louse) PP27267 Pristionchus pacificus (nematode) ENSPTRP00000022133 Pan troglodytes (chimpanzee) ENSRNOP00000026941 Rattus norvegicus (norway rat) XP_787033.2 Strongylocentrotus purpuratus (purple sea urchin) XP_966368.2 Tribolium castaneum (red flour beetle) ENSTNIP00000011176 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000008607 Takifugu rubripes (pufferfish Takifugu) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 3346201 modMine - Data generated by the modENCODE project. • FBgn0053519 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV50066 NV11575 LH11800 LH11798 LH11794 ISCW023694 FBgn0229302 FBgn0223920 FBgn0206831 FBgn0206830 FBgn0196297 FBgn0196296 FBgn0196295 FBgn0170398 JGI_V11_315830 FBgn0245763 FBgn0148045 FBgn0143428 FBgn0129165 FBgn0112167 FBgn0089082 BGIBMGA013531 ACYPI008736 GB10576 AGAP005471 AAEL013559 PB24558 PB24563 PB27086 PHUM405120 PHUM405130 GLEAN_02779
Synonyms & Secondary IDs ( 17 )
Reported As
Symbol Synonym	BEST:HL01080 (White, 2000.2.23, White et al., 1999, ) CG3901 (Kashevsky et al., 2002, ) CG18019   CG18020 (Arbeitman et al., 2002, ) CG18021 (Misra, 2001.8.10, ) CG30171 (Kiger et al., 2003, Giot et al., 2003, Vogel et al., 2003, Burkart et al., 2007) CG30175   CG33519 (FlyBase, 1996-, Morrison et al., 2000, Keleman et al., 2009.8.5, Burkart et al., 2007, Ni et al., 2010.12.1, Cammarato et al., 2011) CT12925 (Hynes and Zhao, 2000) CT40322 (Hynes and Zhao, 2000) CT40326 (Hynes and Zhao, 2000) obs (Burkart et al., 2007) Unc-89 (Sutter et al., 2004, Wasbrough et al., 2010) UNC-89 (Russell and Geisler, 2006.3.3) unc-89 (Schnorrer et al., 2010)
Name Synonym	obscurin (Burkart et al., 2007, Miller et al., 2008) PROJECTIN-like (Morrison et al., 2000) Unc-89 (Cammarato et al., 2011)
Secondary FlyBase IDs
	FBgn0034952 FBgn0034953 FBgn0034954 FBgn0034955 FBgn0040499 FBgn0050171 FBgn0050175 FBpp0072107 FBpp0072108 FBpp0072109 FBtr0072198 FBtr0072200
References ( 38 )
Generate a list of	All references relating to this gene ( 38 )
List References by type	Research paper  ( 8 ) Supplementary material  ( 10 ) Personal communication to FlyBase  ( 9 ) FlyBase analysis  ( 5 ) DNA/RNA sequence record  ( 1 ) Computer file  ( 3 ) Automatic genome annotation  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 1 )
	Schnorrer et al., 2010, Nature 464(7286): 287--291 Systematic genetic analysis of muscle morphogenesis and function in Drosophila. [FBrf0210226] Show all research papers ...