FlyBase Gene Report: Dmel\CG34031

NK-LIKE HOMEOBOX TRANSCRIPTION FACTORS -: NK-like (NKL) homeobox transcription factors are sequence-specific DNA binding proteins that regulate transcription. NKL transcription factors are homeobox genes closely related to Hox-like genes, a number of which are found in the NK cluster. Many of the NKL members contain an Engrailed Homology 1 (EH1) motif. (Adapted from FBrf0232555 and PMID:22094586).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\CG34031 or the JBrowse view of Dmel\CG34031 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.56

Gene model reviewed during 5.46

Gene model reviewed during 5.40

Gene model reviewed during 6.02

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

(FlyBase Genome Annotators, 2011)

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

CG34031-RC

FBtr0308948

NM_001259965

740

219

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

CG34031-PC

FBpp0301047

25.3

219

10.79

NP_001246894

AFH04565

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

M9NDG6

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CG34031 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG34031_1

Linkouts

Gene Ontology (2 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

sequence-specific DNA binding

inferred from electronic annotation with InterPro:IPR017970

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Biological Process (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

regulation of transcription, DNA-templated

inferred from electronic annotation with InterPro:IPR017970

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Cellular Component (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\CG34031 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\CG34031

View exonic expression by tissue for Dmel\CG34031

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0054031

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

HDC11617

Flygut - An atlas of the Drosophila adult midgut

FBgn0054031

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 0 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 2 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of CG34031

Transgenic constructs containing regulatory region of CG34031

Deletions and Duplications ( 0 )

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Scer\GAL4^Tub.PU

CG34031^KK115531

Sterility

Allele

fertile, with Scer\GAL4^Tub.PU

CG34031^KK115531

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (13)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\BARHL1

1 of 15

Yes

Hsap\BARHL2

1 of 15

Yes

Hsap\BARX1

1 of 15

Yes

Hsap\BARX2

1 of 15

Yes

Hsap\DBX1

1 of 15

Yes

Hsap\DBX2

1 of 15

Yes

Hsap\HLX

1 of 15

Yes

Hsap\LBX1

1 of 15

Yes

Hsap\LBX2

1 of 15

Yes

Hsap\NKX1-1

1 of 15

Yes

Hsap\TLX1

1 of 15

Yes

Hsap\TLX2

1 of 15

Yes

Hsap\TLX3

1 of 15

Yes

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (14)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Barhl1

1 of 15

Yes

Mmus\Barhl2

1 of 15

Yes

Mmus\Barx1

1 of 15

Yes

Mmus\Barx2

1 of 15

Yes

Mmus\Dbx1

1 of 15

Yes

Mmus\Dbx2

1 of 15

Yes

Mmus\Hlx

1 of 15

Yes

Mmus\Lbx1

1 of 15

Yes

Mmus\Lbx2

1 of 15

Yes

Mmus\Nkx1-1

1 of 15

Yes

Mmus\Nkx1-2

1 of 15

Yes

Mmus\Tlx1

1 of 15

Yes

Mmus\Tlx2

1 of 15

Yes

Mmus\Tlx3

1 of 15

Yes

Rattus norvegicus (Norway rat) (13)

Rnor\Barhl1

1 of 13

Yes

Rnor\Barhl2

1 of 13

Yes

Rnor\Barx1

1 of 13

Yes

Rnor\Barx2

1 of 13

Yes

Rnor\Dbx1

1 of 13

Yes

Rnor\Dbx2

1 of 13

Yes

Rnor\Hlx

1 of 13

Yes

Rnor\Lbx1

1 of 13

Yes

Rnor\LOC103692168

1 of 13

Yes

Rnor\Nkx1-1

1 of 13

Yes

Rnor\Nkx1-2

1 of 13

Yes

Rnor\Tlx1

1 of 13

Yes

Rnor\Tlx3

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (11)

Xtro\barhl1

1 of 12

Yes

Xtro\barhl2

1 of 12

Yes

Xtro\barx1

1 of 12

Yes

Xtro\dbx1

1 of 12

Yes

Xtro\hlx

1 of 12

Yes

Xtro\nkx1-1

1 of 12

Yes

Xtro\nkx1-2

1 of 12

Yes

Xtro\tlx1

1 of 12

Yes

Xtro\tlx2

1 of 12

Yes

Xtro\tlx3

1 of 12

Yes

Xtro\ventx1.2

1 of 12

Yes

Danio rerio (Zebrafish) (17)

Drer\barhl1b

1 of 15

Yes

Drer\barhl2

1 of 15

Yes

Drer\barx1

1 of 15

Yes

Drer\barx2

1 of 15

Yes

Drer\dbx1b

1 of 15

Yes

Drer\hlx1

1 of 15

Yes

Drer\lbx1a

1 of 15

Yes

Drer\lbx1b

1 of 15

Yes

Drer\lbx2

1 of 15

Yes

Drer\LOC100008066

1 of 15

Yes

Drer\nkx1.2lb

1 of 15

Yes

Drer\pnx

1 of 15

Yes

Drer\tlx1

1 of 15

Yes

Drer\tlx2

1 of 15

Yes

Drer\tlx3b

1 of 15

Yes

Drer\ved

1 of 15

Yes

Drer\vox

1 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (3)

Cele\ceh-19

3 of 15

Yes

Cele\ceh-30

1 of 15

Cele\ceh-31

1 of 15

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190GD6 )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila simulans

Drosophila simulans

Drosophila erecta

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA31647

Drosophila willistoni

Dwil\GK28065

Drosophila virilis

Dvir\GJ26136

Drosophila mojavensis

Dmoj\GI23625

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150BVN )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17004364

Mayetiola destructor

Hessian fly

Mdes\Mdes007026

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ011541

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W05QE )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA012499

Danaus plexippus

Monarch butterfly

Dple\DPOGS201237-PA

Apis florea

Little honeybee

Aflo\2147574019

Apis mellifera

Western honey bee

Amel\LOC726825

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP14923

Bombus terrestris

Buff-tailed bumblebee

Bter\15203977

Linepithema humile

Argentine ant

Lhum\LH21788

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:002432

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG008875

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_08078

Tribolium castaneum

Red flour beetle

Tcas\TC001164

Pediculus humanus

Human body louse

Phum\PHUM606500

Rhodnius prolixus

Kissing bug

Rpro\RPRC000954

Cimex lectularius

Bed bug

Clec\CLEC003809

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI42465

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_18185

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X05MD )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR006886

Tetranychus urticae

Two-spotted spider mite

Turt\tetur11g02100

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_29136

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G08M2 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Phb2

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (16)

Gene Symbol

Score

Source

Alignment

2 of 10

1 of 10

1 of 10

btn

1 of 10

C15

1 of 10

CG11085

1 of 10

Dbx

1 of 10

eve

1 of 10

exex

1 of 10

H2.0

1 of 10

lbe

1 of 10

lbl

1 of 10

lms

1 of 10

NK7.1

1 of 10

unpg

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0054031

Pathways

Gene Group - Pathway Membership (FlyBase)

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-47

Cytogenetic map

80D4-80D4

Sequence location

3L:23,174,446..23,185,750 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

80D4-80D4

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-47

(FlyBase, 2016)

3-44.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (2)

Bloomington

57454

y¹ sc^* v¹ sev²¹; P{TRiP.HMC04762}attP40

VDRC

v109096

P{KK115531}VIE-260B

Genomic Clones (11)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (12)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0054031

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

3885665

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: CG34031 BP1068

(FlyBase Genome Annotators, 2005)

Source for database merge of

Additional comments

Other Comments

Annotation CR34031 renamed CG34031 in release 5.40 of the genome annotation.

(FlyBase Genome Annotators, 2011)

Annotation CR34031 reclassified as a pseudogene in release 5.26 of the genome annotation (renamed CR34031 in the prior release).

(FlyBase Genome Annotators, 2010)

New annotation (CG34031) in release 4.3 of the genome annotation.

(FlyBase Genome Annotators, 2005)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 22 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

3885665

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

AFH04565

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

M9NDG6

Other crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG34031_1

Flygut - An atlas of the Drosophila adult midgut

FBgn0054031

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

3885665

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC001164

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG34031

modMine - A data warehouse for the modENCODE project

FBgn0054031

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0054031

DRSC - Results frm RNAi screens

FBgn0054031

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0054031

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

HDC11617

FlyMine - An integrated database for Drosophila genomics

FBgn0054031

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

BP1068

(Yandell et al., 2005)

CG34031

(Moskalev et al., 2019, Syrzycka et al., 2019, Bürglin and Affolter, 2016, FlyBase Genome Annotators, 2011, Ni et al., 2010.12.1, Keleman et al., 2009.8.5)

CR34031

(FlyBase Genome Annotators, 2010)

Name Synonyms

Secondary FlyBase IDs

FBan0034031
FBgn0082838

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (16)

Report Sections

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