FB2020_03, released Jun 16, 2020

Gene: Dmel\Ube3a

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General Information
Symbol
Dmel\Ube3a
Species
D. melanogaster
Name
Ubiquitin protein ligase E3A
Annotation Symbol
CG6190
Feature Type
protein_coding_gene
FlyBase ID
FBgn0061469
Gene Model Status
Current
Stock Availability
16 publicly available
Gene Snapshot
Ubiquitin protein ligase E3A (Ube3a) encodes the founding member of the HECT-type ubiquitin E3 ligase family of enzymes. It is involved in the final step of conjugation of ubiquitin to its target substrates. It regulates protein degradation by targeting modified proteins to the proteasome or by regulating the proteasome activity through ubiquitination of its subunits, which in turn affects many aspects of neuronal function, such as synaptic plasticity, long-term memory or dendritic development. [Date last reviewed: 2019-02-28]
Other Summaries
Also Known As

dube3a, Angelman syndrome, As, Drosophila Angelman syndrome, das

Key Links
Genomic Location
Cytogenetic map
68B1-68B1
Sequence location
3L:11,206,422..11,210,583 [-]
Recombination map

3-35

RefSeq locus
NT_037436 REGION:11206422..11210583
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (13 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
ubiquitin protein ligase activity
inferred from direct assay
(Li et al., 2016)
inferred from mutant phenotype
(Lee et al., 2014)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (10 terms)
Terms Based on Experimental Evidence (10 terms)
CV Term
Evidence
References
circadian rhythm
inferred from mutant phenotype
(Wu et al., 2008)
dendrite morphogenesis
inferred from mutant phenotype
(Lu et al., 2009)
locomotion
inferred from mutant phenotype
(Wu et al., 2008)
long-term memory
inferred from mutant phenotype
(Wu et al., 2008)
negative regulation of BMP signaling pathway
inferred from mutant phenotype
(Li et al., 2016)
inferred from genetic interaction with FLYBASE:tkv; FB:FBgn0003716
(Li et al., 2016)
inferred from genetic interaction with FLYBASE:Mad; FB:FBgn0011648
(Li et al., 2016)
negative regulation of synaptic growth at neuromuscular junction
inferred from mutant phenotype
(Li et al., 2016)
peripheral nervous system neuron development
inferred from mutant phenotype
(Lu et al., 2009)
proteasome-mediated ubiquitin-dependent protein catabolic process
inferred from genetic interaction with FLYBASE:Rpn10; FB:FBgn0015283
(Lee et al., 2014)
inferred from direct assay
(Li et al., 2016)
protein polyubiquitination
inferred from mutant phenotype
(Lee et al., 2014)
ubiquitin-dependent protein catabolic process
inferred from mutant phenotype
(Lee et al., 2014)
Terms Based on Predictions or Assertions (0 terms)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
cytoplasm
inferred from direct assay
(Wu et al., 2008)
cytosol
inferred from direct assay
(Li et al., 2016)
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Pathway (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
HECT UBIQUITIN LIGASES -
E3 (ubiquitin) ligases interact with both ubiquitin-E2 (ubiquitin-conjugating) enzymes and the target protein, transferring the ubiquitin from the E2 to the substrate. Members of the HECT family of E3 ubiquitin ligases are characterized by a C-terminal HECT domain. This catalytic domain forms an intermediate thioester bond with ubiquitin via a conserved Cys when transferring ubiquitin from the E2 to the target protein. (Adapted from FBrf0225009).
Pathway (FlyBase)
Negative Regulators of BMP Signaling Pathway -
Negative regulators of Bone Morphogenetic Protein (BMP) signaling down-regulate the pathway, ultimately resulting in the decreased nuclear activity of the Mad/Med transcription factor complex.
Summary (Interactive Fly)

Ubiquitination - protein degradation - model for Angelman syndrome - neuromuscular junction - regulation of the actin cytoskeleton, the formation of terminal dendritic branches, and dopamine/serotonin synthesis

Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\Ube3a or the JBrowse view of Dmel\Ube3a for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.45

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Ube3a-RA
FBtr0076186
NM_140195
3674
973
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
Ube3a-PA
FBpp0075916
107.6
973
4.40
NP_648452
AAF50078
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ube3a using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism
(Fisher et al., 2012)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 5
embryonic cortex | ubiquitous
(Wu et al., 2008)
embryonic stage
organism | ubiquitous
(Wu et al., 2008)
larval stage
organism | ubiquitous
(Wu et al., 2008)
third instar larval stage
ventral nerve cord
(Li et al., 2016)
embryonic/larval motor neuron of ventral nerve cord
(Li et al., 2016)
interneuron of ventral nerve cord
(Li et al., 2016)
neuromuscular junction
(Li et al., 2016)
oogenesis & adult stage | female
ovary
(Li et al., 2016)
spermatogenesis & adult stage | male
testis
(Li et al., 2016)
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
third instar larval stage
eye disc
(Wu et al., 2008)
Additional Descriptive Data

Ube3a protein is broadly expressed, and mainly localized to the cytoplasm. Expression at post-embryonic stages is insufficiently robust to detect using immunocytochemistry. Ube3a protein is ubiquitously expressed in third star larvae, and is cytoplasmically localized. In the larval ventral nerve cord and brain , Ube3a protein expression is elevated in neuroblasts that are too young to express elav protein. Ube3a protein is expressed broadly in the adult brain, with particularly high levels in the mushroom bodies.

(Wu et al., 2008)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
cytoplasm
inferred from direct assay
(Wu et al., 2008)
cytosol
inferred from direct assay
(Li et al., 2016)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Ube3a in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 16 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 28 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Ube3a
Transgenic constructs containing regulatory region of Ube3a
Deletions and Duplications ( 4 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal, with Scer\GAL4αTub84B.PL
lethal | embryonic stage, with Scer\GAL4VP16.mat.αTub67C
lethal | first instar larval stage, with Scer\GAL4VP16.mat.αTub67C
lethal | male limited, with Scer\GAL4elav-C155
lethal | recessive
viable
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
viable, with Scer\GAL4αTub84B.PL
viable (with Df(3L)ED4470)
viable (with Df(3L)vin5)
Sterility
Allele
fertile
fertile (with Df(3L)ED4470)
fertile (with Df(3L)vin5)
Other Phenotypes
Allele
bang sensitive | adult stage | progressive, with Scer\GAL4repo
chemosensitive behavior defective | adult stage
endocytosis defective | third instar larval stage
learning defective | adult stage
learning defective | adult stage, with Scer\GAL4elav.PU
learning defective | adult stage, with Scer\GAL4ey-OK107
learning defective | adult stage, with Scer\GAL4Tab2-201Y
learning defective | adult stage | adult stage | RU486 conditional, with Scer\GAL4elav.Switch.PO
learning defective | adult stage | adult stage | RU486 conditional, with Scer\GAL4Mef2.247.Switch
learning defective | adult stage | dominant | maternal effect
learning defective | dominant | larval stage | maternal effect
locomotor behavior defective
locomotor behavior defective, with Scer\GAL4elav-C155
locomotor behavior defective | adult stage
locomotor behavior defective | adult stage, with Scer\GAL4elav.PLu
locomotor rhythm defective
memory defective
memory defective | adult stage
memory defective | adult stage | RU486 conditional, with Scer\GAL4elav.Switch.PO
memory defective | adult stage | RU486 conditional, with Scer\GAL4Mef2.247.Switch
neuroanatomy defective
neuroanatomy defective, with Scer\GAL4221
neuroanatomy defective, with Ube3aGD450, Scer\GAL4477
neuroanatomy defective, with Ube3aΔ15-8, Scer\GAL4477
neuroanatomy defective (with Ube3aΔ3-8)
neuroanatomy defective (with Ube3aΔ15-8)
neuroanatomy defective | adult stage | recessive
neuroanatomy defective | third instar larval stage
neuroanatomy defective | third instar larval stage, with Scer\GAL4elav.PU
neuroanatomy defective | third instar larval stage, with Scer\GAL4elav-C155
neuroanatomy defective | third instar larval stage, with Scer\GAL4RapGAP1-OK6
neuroanatomy defective | third instar larval stage (with Df(3L)ED4470)
neuroanatomy defective | third instar larval stage (with Ube3a15B)
neuroanatomy defective | third instar larval stage (with Ube3a35)
neuroanatomy defective | third instar larval stage (with Ube3a549)
neurophysiology defective | adult stage, with Scer\GAL4repo
neurophysiology defective | third instar larval stage
neurophysiology defective | third instar larval stage, with Scer\GAL4elav-C155
paralytic | adult stage | conditional, with Scer\GAL4repo
paralytic | adult stage | heat sensitive, with Scer\GAL4repo
size defective, with Scer\GAL4GMR.PU
visible, with Scer\GAL4GMR.PU
wild-type
Phenotype manifest in
Allele
adult mushroom body, with Scer\GAL4repo
adult mushroom body alpha-lobe
adult optic lobe | adult stage, with Scer\GAL4repo
embryonic/larval neuromuscular junction | third instar larval stage
embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4elav-C155
eye, with Scer\GAL4GMR.PF
eye, with Scer\GAL4GMR.PU
interommatidial bristle, with Scer\GAL4GMR.PF
larval dorsal multidendritic neuron ddaC
larval dorsal multidendritic neuron ddaC, with Ube3aGD450, Scer\GAL4477
larval dorsal multidendritic neuron ddaC, with Ube3aΔ15-8, Scer\GAL4477
larval dorsal multidendritic neuron ddaC (with Ube3aΔ3-8)
larval dorsal multidendritic neuron ddaC (with Ube3aΔ15-8)
larval dorsal multidendritic neuron ddaE
larval dorsal multidendritic neuron ddaE, with Scer\GAL4221
larval dorsal multidendritic neuron ddaF
larval dorsal multidendritic neuron ddaF, with Scer\GAL4221
mushroom body beta-lobe
NMJ bouton | supernumerary | third instar larval stage
NMJ bouton | supernumerary | third instar larval stage, with Scer\GAL4elav.PU
NMJ bouton | supernumerary | third instar larval stage, with Scer\GAL4RapGAP1-OK6
NMJ bouton | supernumerary | third instar larval stage (with Df(3L)ED4470)
NMJ bouton | supernumerary | third instar larval stage (with Ube3a15B)
NMJ bouton | supernumerary | third instar larval stage (with Ube3a35)
NMJ bouton | third instar larval stage (with Df(3L)ED4470)
NMJ bouton | third instar larval stage (with Ube3a35)
NMJ bouton | third instar larval stage (with Ube3a549)
ommatidium, with Scer\GAL4GMR.PF
photoreceptor cell, with Scer\GAL4GMR.PF
retina, with Scer\GAL4GMR.PF
synaptic vesicle | third instar larval stage
synaptic vesicle | third instar larval stage, with Scer\GAL4elav-C155
wing, with Scer\GAL4Bx-MS1096
wing margin, with Scer\GAL4vg.PM
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (38)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\UBE3A
14 of 15
Yes
Yes
 
5  
Hsap\HECTD2
3 of 15
No
No
Hsap\HERC3
2 of 15
No
No
1  
Hsap\HERC4
2 of 15
No
No
1  
Hsap\HERC5
2 of 15
No
No
Hsap\HERC6
2 of 15
No
No
Hsap\AREL1
1 of 15
No
No
Hsap\ASCC1
1 of 15
No
No
Hsap\BAG3
1 of 15
No
No
Hsap\GAS7
1 of 15
No
No
Hsap\HACE1
1 of 15
No
No
Hsap\HECTD1
1 of 15
No
No
Hsap\HECTD3
1 of 15
No
No
Hsap\HECW1
1 of 15
No
No
Hsap\HECW2
1 of 15
No
No
Hsap\HUWE1
1 of 15
No
No
1  
Hsap\ITCH
1 of 15
No
No
Hsap\MAGI1
1 of 15
No
No
Hsap\MAGI2
1 of 15
No
No
Hsap\MAGI3
1 of 15
No
No
Hsap\MAGIX
1 of 15
No
No
Hsap\NEDD4
1 of 15
No
No
Hsap\NEDD4L
1 of 15
No
No
Hsap\PLEKHA5
1 of 15
No
No
Hsap\PLEKHA7
1 of 15
No
No
Hsap\SMURF1
1 of 15
No
No
Hsap\SMURF2
1 of 15
No
No
Hsap\TRIP12
1 of 15
No
No
1  
Hsap\UBE3B
1 of 15
No
No
Hsap\UBE3C
1 of 15
No
No
Hsap\UBR5
1 of 15
No
No
Hsap\WWC1
1 of 15
No
No
Hsap\WWC2
1 of 15
No
No
Hsap\WWC3
1 of 15
No
No
1  
Hsap\WWP1
1 of 15
No
No
2  
Hsap\WWP2
1 of 15
No
No
Hsap\WWTR1
1 of 15
No
No
Hsap\YAP1
1 of 15
No
No
14  
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (34)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Ube3a
14 of 15
Yes
Yes
Mmus\Hectd2
3 of 15
No
No
Mmus\Herc4
2 of 15
No
No
Mmus\Herc6
2 of 15
No
No
Mmus\Arel1
1 of 15
No
No
Mmus\Bag3
1 of 15
No
No
Mmus\Gas7
1 of 15
No
No
Mmus\Hace1
1 of 15
No
No
Mmus\Hectd3
1 of 15
No
No
Mmus\Hecw1
1 of 15
No
No
Mmus\Hecw2
1 of 15
No
No
Mmus\Herc3
1 of 15
No
No
Mmus\Huwe1
1 of 15
No
No
Mmus\Itch
1 of 15
No
No
Mmus\Magi1
1 of 15
No
No
Mmus\Magi2
1 of 15
No
No
Mmus\Magi3
1 of 15
No
No
Mmus\Magix
1 of 15
No
No
Mmus\Nedd4l
1 of 15
No
No
Mmus\Nedd4
1 of 15
No
No
Mmus\Plekha5
1 of 15
No
No
Mmus\Plekha7
1 of 15
No
No
Mmus\Smurf1
1 of 15
No
No
Mmus\Smurf2
1 of 15
No
No
Mmus\Trip12
1 of 15
No
No
Mmus\Ube3b
1 of 15
No
No
Mmus\Ube3c
1 of 15
No
No
Mmus\Ubr5
1 of 15
No
No
Mmus\Wwc1
1 of 15
No
No
Mmus\Wwc2
1 of 15
No
No
Mmus\Wwp1
1 of 15
No
No
Mmus\Wwp2
1 of 15
No
No
Mmus\Wwtr1
1 of 15
No
No
Mmus\Yap1
1 of 15
No
No
Rattus norvegicus (Norway rat) (31)
Rnor\Ube3a
13 of 13
Yes
Yes
Rnor\Herc3
2 of 13
No
No
Rnor\Herc6
2 of 13
No
No
Rnor\Arel1
1 of 13
No
No
Rnor\Bag3
1 of 13
No
No
Rnor\Hace1
1 of 13
No
No
Rnor\Hectd1
1 of 13
No
No
Rnor\Hectd2
1 of 13
No
No
Rnor\Hectd3
1 of 13
No
No
Rnor\Hecw1
1 of 13
No
No
Rnor\Hecw2
1 of 13
No
No
Rnor\Herc4
1 of 13
No
No
Rnor\Magi1
1 of 13
No
No
Rnor\Magi2
1 of 13
No
No
Rnor\Magi3
1 of 13
No
No
Rnor\Magix
1 of 13
No
No
Rnor\Nedd4l
1 of 13
No
No
Rnor\Plekha5
1 of 13
No
No
Rnor\Plekha7
1 of 13
No
Yes
Rnor\Smurf1
1 of 13
No
No
Rnor\Smurf2
1 of 13
No
No
Rnor\Trip12
1 of 13
No
No
Rnor\Ube3b
1 of 13
No
No
Rnor\Ube3c
1 of 13
No
No
Rnor\Ubr5
1 of 13
No
No
Rnor\Wwc1
1 of 13
No
No
Rnor\Wwc2
1 of 13
No
No
Rnor\Wwp1
1 of 13
No
No
Rnor\Wwp2
1 of 13
No
No
Rnor\Wwtr1
1 of 13
No
No
Rnor\Yap1
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (27)
Xtro\ube3a
9 of 12
Yes
Yes
Xtro\herc3
2 of 12
No
No
Xtro\herc4
2 of 12
No
No
Xtro\herc6
2 of 12
No
No
Xtro\arel1
1 of 12
No
No
Xtro\bag3
1 of 12
No
No
Xtro\hace1
1 of 12
No
No
Xtro\hectd1
1 of 12
No
No
Xtro\hectd2
1 of 12
No
No
Xtro\hectd3
1 of 12
No
No
Xtro\hecw1
1 of 12
No
No
Xtro\itch
1 of 12
No
No
Xtro\LOC101730663
1 of 12
No
Yes
Xtro\magi1
1 of 12
No
No
Xtro\magi2
1 of 12
No
No
Xtro\magi3
1 of 12
No
No
Xtro\nedd4l
1 of 12
No
No
Xtro\nedd4
1 of 12
No
No
Xtro\smurf1
1 of 12
No
No
Xtro\trip12
1 of 12
No
No
Xtro\ube3b
1 of 12
No
No
Xtro\ube3c
1 of 12
No
No
Xtro\wwc1
1 of 12
No
No
Xtro\wwc2
1 of 12
No
No
Xtro\wwc3
1 of 12
No
No
Xtro\wwp2
1 of 12
No
No
Xtro\wwtr1
1 of 12
No
No
Danio rerio (Zebrafish) (40)
Drer\ube3a
10 of 15
Yes
Yes
Drer\hectd2
3 of 15
No
No
Drer\herc3
2 of 15
No
No
Drer\herc4
2 of 15
No
No
Drer\si:ch73-190m4.1
2 of 15
No
No
Drer\si:ch211-281g13.4
2 of 15
No
No
Drer\si:ch211-281g13.5
2 of 15
No
No
Drer\slc47a2.2
2 of 15
No
No
Drer\arel1
1 of 15
No
No
Drer\bag3
1 of 15
No
No
Drer\hace1
1 of 15
No
No
Drer\hectd1
1 of 15
No
No
Drer\hecw1b
1 of 15
No
No
Drer\hecw2a
1 of 15
No
No
Drer\hecw2b
1 of 15
No
No
Drer\huwe1
1 of 15
No
No
Drer\itcha
1 of 15
No
No
Drer\LOC556872
1 of 15
No
No
Drer\LOC564220
1 of 15
No
No
Drer\LOC100334952
1 of 15
No
No
Drer\magi1a
1 of 15
No
No
Drer\magi2a
1 of 15
No
No
Drer\magi2b
1 of 15
No
No
Drer\magi3a
1 of 15
No
No
Drer\magixa
1 of 15
No
No
Drer\nedd4a
1 of 15
No
No
Drer\nedd4l
1 of 15
No
No
Drer\plekha5
1 of 15
No
No
Drer\plekha7a
1 of 15
No
No
Drer\plekha7b
1 of 15
No
No
Drer\smurf1
1 of 15
No
No
Drer\smurf2
1 of 15
No
No
Drer\trip12
1 of 15
No
No
Drer\ube3b
1 of 15
No
No
Drer\ube3c
1 of 15
No
No
Drer\ubr5
1 of 15
No
No
Drer\wwc3
1 of 15
No
No
Drer\wwp2
1 of 15
No
No
Drer\wwtr1
1 of 15
No
No
Drer\yap1
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (11)
Cele\herc-1
2 of 15
Yes
No
Cele\eel-1
1 of 15
No
No
Cele\etc-1
1 of 15
No
No
Cele\hecd-1
1 of 15
No
No
Cele\hecw-1
1 of 15
No
No
Cele\magi-1
1 of 15
No
No
Cele\oxi-1
1 of 15
No
No
Cele\wwp-1
1 of 15
No
No
Cele\Y48G8AL.13
1 of 15
No
Yes
Cele\Y92H12A.2
1 of 15
No
No
Cele\yap-1
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (3)
Atha\AT3G02510
1 of 9
Yes
No
Atha\AT5G16040
1 of 9
Yes
No
Atha\UVR8
1 of 9
Yes
No
Saccharomyces cerevisiae (Brewer's yeast) (5)
Scer\HUL4
5 of 15
Yes
No
Scer\HUL5
1 of 15
No
No
Scer\RSP5
1 of 15
No
No
Scer\TOM1
1 of 15
No
No
Scer\UFD4
1 of 15
No
No
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\mug30
4 of 12
Yes
Yes
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG0919024T )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00013003
Drosophila simulans
Dsim\GD12813
Drosophila sechellia
Dsec\GM24764
Drosophila erecta
Dere\GG13928
Drosophila yakuba
Dyak\GE20227
Drosophila ananassae
Dana\GF24560
Drosophila pseudoobscura pseudoobscura
Dpse\GA19425
Drosophila willistoni
Dwil\GK16632
Drosophila virilis
Dvir\GJ11295
Drosophila mojavensis
Dmoj\GI11616
Drosophila grimshawi
Dgri\GH16658
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915027H )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17005485
Musca domestica
House fly
Mdoa\17004452
Musca domestica
House fly
Mdoa\17007483
Glossina morsitans
Tsetse fly
Gmor\GMOY011091
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_08477
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_10387
Mayetiola destructor
Hessian fly
Mdes\Mdes000317
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL012498
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL012500
Anopheles darlingi
American malaria mosquito
Adar\ADAC000429
Anopheles darlingi
American malaria mosquito
Adar\ADAC000438
Anopheles gambiae
Malaria mosquito
Agam\AGAP012366
Anopheles gambiae
Malaria mosquito
Agam\AGAP012365
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ001453
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ001454
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W01ZY )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA001267
Danaus plexippus
Monarch butterfly
Dple\DPOGS205164-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL005465
Heliconius melpomene
Postman butterfly
Hmel\HMEL009982
Apis florea
Little honeybee
Aflo\2795569860
Apis mellifera
Western honey bee
Amel\GB51221
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP17332
Bombus terrestris
Buff-tailed bumblebee
Bter\15211220
Linepithema humile
Argentine ant
Lhum\LH15843
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:000c0f
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG009268
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_00642
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_00643
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_00164
Tribolium castaneum
Red flour beetle
Tcas\TC011206
Pediculus humanus
Human body louse
Phum\PHUM588800
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI003529
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_04242
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X01XG )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR003231
Ixodes scapularis
Black-legged tick
Isca\ISCW009535
Stegodyphus mimosarum
African social velvet spider
Smim\KFM81417.1
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_340714
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_209652
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G01LV )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Ube3a-3
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Ube3a
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000007494
Gallus gallus
Domestic chicken
Ggal\UBE3A
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (18)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
Herc4
4 of 10
CG3356
2 of 10
CG4238
2 of 10
CG5087
2 of 10
CG42797
2 of 10
HERC2
2 of 10
HUWE1
2 of 10
hyd
2 of 10
Nedd4
2 of 10
Smurf
2 of 10
Su(dx)
2 of 10
Ufd4
2 of 10
CG6678
1 of 10
CG7420
1 of 10
ctrip
1 of 10
kibra
1 of 10
Magi
1 of 10
yki
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 10 )
Potential Models Based on Orthology ( 1 )
Human Ortholog
Disease
Evidence
References
UBE3A; ubiquitin protein ligase E3A
model of  Angelman syndrome
IEA
(FlyBase, 2019-)
Modifiers Based on Experimental Evidence ( 2 )
Allele
Disease
Interaction
References
Ube3aunspecified
model of  intellectual disability
is ameliorated by Hsap\UBE3AUAS.iso1.cCa
(Chakraborty et al., 2015)
is ameliorated by drlUAS.cCa
(Chakraborty et al., 2015)
Ube3a35
model of  Angelman syndrome
is ameliorated by Mad12
(Li et al., 2016)
is ameliorated by tkv8
(Li et al., 2016)
is ameliorated by tkvk16713
(Li et al., 2016)
Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
UBE3A; ubiquitin protein ligase E3A
14 of 15
601623
 
HECTD2; HECT domain E3 ubiquitin protein ligase 2
3 of 15
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Dmel gene
      Ortholog showing functional complementation
      Supporting References
      Ube3a
      Hsap\UBE3A
      (Chakraborty et al., 2015)
      Interactions
      Summary of Physical Interactions
      esyN Network Diagram
      Show neighbor-neighbor interactions:
      Select Layout:
      Legend:
      Protein
      RNA
      Selected Interactor(s)
      Interactions Browser
      View in Interactions Browser
      Please see the Physical Interaction reports below for full details
      protein-protein
      Physical Interaction
      Assay
      References
      Ube3a - Atpα
      enzymatic study, western blot
      (Jensen et al., 2013)
      Ube3a - S6KL
      anti tag coimmunoprecipitation, anti tag western blot
      (Li et al., 2016)
      Ube3a - pbl
      anti tag coimmunoprecipitation, anti tag western blot
      (Reiter et al., 2006)
      Ube3a - tkv
      enzymatic study, western blot, anti tag coimmunoprecipitation, anti tag western blot
      (Li et al., 2016)
      Ube3a - trpl
      anti tag coimmunoprecipitation, anti tag western blot
      (Zhang et al., 2013)
      Summary of Genetic Interactions
      esyN Network Diagram
      esyN Network Key:
      Suppression
      Enhancement
      View in Interactions Browser
      Please look at the allele data for full details of the genetic interactions
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Ube3a
      suppressible
      Atpα
      (Hope et al., 2017)
      Ube3a
      suppressible
      drl
      (Chakraborty et al., 2015)
      Ube3a
      enhanceable
      eff
      (Lu et al., 2009)
      Ube3a
      suppressible
      Mad
      (Li et al., 2016)
      Ube3a
      enhanceable
      Pu
      (Ferdousy et al., 2011)
      Ube3a
      suppressible
      Rpn10
      (Lee et al., 2014)
      Ube3a
      suppressible
      tkv
      (Li et al., 2016)
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      eff
      enhanceable
      Ube3a
      (Lu et al., 2009)
      External Data
      Linkouts
      DroID - A comprehensive database of gene and protein interactions.
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Pathways
      Signaling Pathways (FlyBase)
      Negative Regulators of BMP Signaling Pathway -
      Negative regulators of Bone Morphogenetic Protein (BMP) signaling down-regulate the pathway, ultimately resulting in the decreased nuclear activity of the Mad/Med transcription factor complex.
      Metabolic Pathways
      External Data
      Linkouts
      KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
      Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      3L
      Recombination map

      3-35

      Cytogenetic map
      68B1-68B1
      Sequence location
      3L:11,206,422..11,210,583 [-]
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      68B1-68B1
      Limits computationally determined from genome sequence between P{PZ}l(3)0123901239&P{lacW}l(3)01239j9B4 and P{PZ}CycA02461&P{PZ}CycA03946
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      Experimentally Determined Recombination Data
      Location

      3-35

      (FlyBase, 2016)

      3-28.9

      (Comeron et al., 2012)
      Left of (cM)
      Right of (cM)
      Notes
      Stocks and Reagents
      Stocks (16)
      Genomic Clones (15)
       

      Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

      cDNA Clones (68)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequenced
      BDGP DGC clones
      Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        Antibody Information
        Laboratory Generated Antibodies
         

        monoclonal

        (Li et al., 2016)

        polyclonal

        (Lu et al., 2009, Wu et al., 2008)
        Commercially Available Antibodies
         
        Other Information
        Relationship to Other Genes
        Source for database identify of

        Source for identity of: Ube3a As

        (Reiter et al., 2006)
        Source for database merge of

        Source for merge of: As CG6190

        (Bier, 2005)
        Additional comments
        Other Comments

        Ube3a regulates monoamine synthesis by increasing GTP cyclohydrolase 1 activity via a non-ubiquitin ligase mechanism.

        (Ferdousy et al., 2011)

        One of 42 Drosophila genes identified as being most likely to reveal molecular and cellular mechanisms of nervous system development or plasticity relevant to human Mental Retardation disorders.

        (Inlow and Restifo, 2004)

        Misexpression of As in the wing results in ectopic wing veins and blisters.

        (Bowers et al., 2002)
        Origin and Etymology
        Discoverer
        Etymology
        Identification
        External Crossreferences and Linkouts ( 36 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
        Other crossreferences
        BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
        Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
        Flygut - An atlas of the Drosophila adult midgut
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
        InterPro - A database of protein families, domains and functional sites
        KEGG Genes - Molecular building blocks of life in the genomic space.
        modMine - A data warehouse for the modENCODE project
        Linkouts
        DroID - A comprehensive database of gene and protein interactions.
        DRSC - Results frm RNAi screens
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
        FlyMine - An integrated database for Drosophila genomics
        Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        Synonyms and Secondary IDs (20)
        Reported As
        Symbol Synonym
        AS
        (Wu et al., 2008)
        As
        (Franco et al., 2011, Arancio et al., 2010)
        CG6190
        (Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Lu et al., 2009, Perkins et al., 2009, Wu et al., 2008, Wu et al., 2008, Goodman et al., 2006, Molnar et al., 2006, Muraro and Moffat, 2006, Peterson and Reiter, 2006, Reiter et al., 2006, Brumby et al., 2004, Inlow and Restifo, 2004, Driscoll and Gerstbrein, 2003)
        Dube3A
        (Androschuk et al., 2015)
        Dube3a
        (Hope et al., 2019, Hope et al., 2017, Hope et al., 2016, Valdez et al., 2015, Jensen et al., 2013, Summers et al., 2008)
        NM_140195.2
        (Marin, 2010)
        UBE3A
        (Ferdousy et al., 2011, Lu et al., 2009, Cukier et al., 2008)
        Ube3a
        (Reiter, 2020.5.3, Singh et al., 2020, Straub et al., 2020, Ueoka et al., 2019, Ramirez et al., 2018, Ketosugbo et al., 2017, Transgenic RNAi Project members, 2017-, Crocker et al., 2016, Dong et al., 2016, Ramirez et al., 2015, Gossan et al., 2014, Lee et al., 2014, Jensen et al., 2013, Kim et al., 2013, Du et al., 2011)
        dUBE3A
        (Jana, 2012, Lu et al., 2009)
        dUbe3A
        (Copf, 2015)
        dUbe3a
        (Kim et al., 2013)
        das
        (Bowers et al., 2002, Bier and Reiter, 2001)
        dube3A
        (Peterson and Reiter, 2006, Bier, 2005)
        dube3a
        (Chakraborty et al., 2015, Okray and Hassan, 2013, Wu et al., 2008, Wu et al., 2008, Kracklauer et al., 2007, Elsisi et al., 2006, Reiter et al., 2006)
        ube3a
        (Cai et al., 2019, Iyer et al., 2016, Li et al., 2016, Oortveld et al., 2013, Zhang et al., 2013)
        Name Synonyms
        Angelman syndrome
        (Lu et al., 2009, Wu et al., 2008, Bowers et al., 2002, Bier and Reiter, 2001)
        Drosophila Angelman syndrome
        (Bowers et al., 2002, Bier and Reiter, 2001)
        Ubiquitin protein ligase E3A
        Ubiquitin-protein ligase E3A
        (Dong et al., 2016)
        ubiquitin protein ligase 3a
        (Oortveld et al., 2013)
        Secondary FlyBase IDs
        • FBgn0036148
        Datasets (0)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        References (84)