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Gene Dmel\GstE8

General Information
Symbol	Dmel\GstE8	Species	D. melanogaster
Name	Glutathione S transferase E8	Annotation symbol	CG17533
Feature type	protein_coding_gene	FlyBase ID	FBgn0063492
Gene Model Status	Current	Stock availability	3 publicly available
Also Known As	DmGSTE8, GST3-2
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	55C7-55C8	Sequence location	2R:14,295,339..14,296,067 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Glutathione S transferase E8 is referred to in FlyBase by the symbol Dmel\GstE8 (CG17533, FBgn0063492). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: glutathione transferase activity. Based on sequence similarity, it is predicted to be involved in the biological process: glutathione metabolic process. 2 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Glutathione S-transferase, C-terminal; Glutathione S-transferase, C-terminal-like; Glutathione S-transferase, N-terminal; Glutathione S-transferase/chloride channel, C-terminal; Thioredoxin-like fold. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-06 and 18-24 hour embryonic stages, at stages throughout the larval period, in stages of adults of both sexes. Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. Comments on Affy2 ProbeSet: ProbeSet 1633238_at partially aligns to an exonic region of the only FlyBase-annotated transcript isoform of GstE8: 3 out of 14 probes fall into this exonic region. Gene sequence location is 2R:14295339..14296067.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03	Controlled Vocabulary Terms cytoplasm
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 55C7-55C8   Limits computationally determined from genome sequence between P{PZ}sbb04525&P{EP}EP1081EP1081 and P{lacW}l(2)08770k04808
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\GstE8 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.43 Gene model reviewed during 5.50
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) GstE8-RA FBtr0086676  NM_137486   729   222
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein GstE8-PA   FBpp0085857   24.9   222   6.02     NP_611330   AAF57694
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Glutathione S-transferase, N-terminal (IPR004045) Glutathione S-transferase, C-terminal (IPR004046) Glutathione S-transferase, C-terminal-like (IPR010987) Thioredoxin-like fold (IPR012336) Glutathione S-transferase/chloride channel, C-terminal (IPR017933)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA696749   AE013599 AAF57694   AJ437579 CAD26834   AW941310   AY075363 AAL68213   CO302921   CO302922   CO307211   CO327089   CO337106   EC074485   EC200526   EC226421   EC234918   EC256916   EC266952
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	A1ZB73 Q8T8R2 Q8T0X2
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    4.95 Larval Midgut    5.8 Larval Hindgut    6.5 Larval Malpighian Tubules    4.2 Larval Fat Body    7.4 Larval Salivary Gland    5.6 Larval Trachea    3.35 Larval Carcass    8 Adult Head    4.9 Adult Eye    4.15 Adult Brain    2.6 Adult Thoracic-Abdominal Ganglion    3.6 Adult Crop    2.5 Adult Midgut    13.7 Adult Hindgut    3.9 Adult Malpighian Tubules    6.4 Adult Fat Body    14.5 Adult Salivary Gland    2.4 Adult Heart    10.225 Adult VirginFemale Spermatheca    21.9 Adult InseminatedFemale Spermatheca    5.2 Adult Ovary    11.2 Adult Testis    5.1 Adult Male Accessory Gland    4.7 Adult Carcass    6.5 Expression Level Scale  None   Low  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    4.95 Larval Midgut    5.8 Larval Hindgut    6.5 Larval Malpighian Tubules    4.2 Larval Fat Body    7.4 Larval Salivary Gland    5.6 Larval Trachea    3.35 Larval Carcass    8 Adult Head    4.9 Adult Eye    4.15 Adult Brain    2.6 Adult Thoracic-Abdominal Ganglion    3.6 Adult Crop    2.5 Adult Midgut    13.7 Adult Hindgut    3.9 Adult Malpighian Tubules    6.4 Adult Fat Body    14.5 Adult Salivary Gland    2.4 Adult Heart    10.225 Adult VirginFemale Spermatheca    21.9 Adult InseminatedFemale Spermatheca    5.2 Adult Ovary    11.2 Adult Testis    5.1 Adult Male Accessory Gland    4.7 Adult Carcass    6.5 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    4.95 Larval Midgut    5.8 Larval Hindgut    6.5 Larval Malpighian Tubules    4.2 Larval Fat Body    7.4 Larval Salivary Gland    5.6 Larval Trachea    3.35 Larval Carcass    8 Adult Head    4.9 Adult Eye    4.15 Adult Brain    2.6 Adult Thoracic-Abdominal Ganglion    3.6 Adult Crop    2.5 Adult Midgut    13.7 Adult Hindgut    3.9 Adult Malpighian Tubules    6.4 Adult Fat Body    14.5 Adult Salivary Gland    2.4 Adult Heart    10.225 Adult VirginFemale Spermatheca    21.9 Adult InseminatedFemale Spermatheca    5.2 Adult Ovary    11.2 Adult Testis    5.1 Adult Male Accessory Gland    4.7 Adult Carcass    6.5 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    4.95 Larval Midgut    5.8 Larval Hindgut    6.5 Larval Malpighian Tubules    4.2 Larval Fat Body    7.4 Larval Salivary Gland    5.6 Larval Trachea    3.35 Larval Carcass    8 Adult Head    4.9 Adult Eye    4.15 Adult Brain    2.6 Adult Thoracic-Abdominal Ganglion    3.6 Adult Crop    2.5 Adult Midgut    13.7 Adult Hindgut    3.9 Adult Malpighian Tubules    6.4 Adult Fat Body    14.5 Adult Salivary Gland    2.4 Adult Heart    10.225 Adult VirginFemale Spermatheca    21.9 Adult InseminatedFemale Spermatheca    5.2 Adult Ovary    11.2 Adult Testis    5.1 Adult Male Accessory Gland    4.7 Adult Carcass    6.5 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    4.95 Larval Midgut    5.8 Larval Hindgut    6.5 Larval Malpighian Tubules    4.2 Larval Fat Body    7.4 Larval Salivary Gland    5.6 Larval Trachea    3.35 Larval Carcass    8 Adult Head    4.9 Adult Eye    4.15 Adult Brain    2.6 Adult Thoracic-Abdominal Ganglion    3.6 Adult Crop    2.5 Adult Midgut    13.7 Adult Hindgut    3.9 Adult Malpighian Tubules    6.4 Adult Fat Body    14.5 Adult Salivary Gland    2.4 Adult Heart    10.225 Adult VirginFemale Spermatheca    21.9 Adult InseminatedFemale Spermatheca    5.2 Adult Ovary    11.2 Adult Testis    5.1 Adult Male Accessory Gland    4.7 Adult Carcass    6.5 Expression Level Scale  None   Low  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    4.95 Larval Midgut    5.8 Larval Hindgut    6.5 Larval Malpighian Tubules    4.2 Larval Fat Body    7.4 Larval Salivary Gland    5.6 Larval Trachea    3.35 Larval Carcass    8 Adult Head    4.9 Adult Eye    4.15 Adult Brain    2.6 Adult Thoracic-Abdominal Ganglion    3.6 Adult Crop    2.5 Adult Midgut    13.7 Adult Hindgut    3.9 Adult Malpighian Tubules    6.4 Adult Fat Body    14.5 Adult Salivary Gland    2.4 Adult Heart    10.225 Adult VirginFemale Spermatheca    21.9 Adult InseminatedFemale Spermatheca    5.2 Adult Ovary    11.2 Adult Testis    5.1 Adult Male Accessory Gland    4.7 Adult Carcass    6.5 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    4.95 Larval Midgut    5.8 Larval Hindgut    6.5 Larval Malpighian Tubules    4.2 Larval Fat Body    7.4 Larval Salivary Gland    5.6 Larval Trachea    3.35 Larval Carcass    8 Adult Head    4.9 Adult Eye    4.15 Adult Brain    2.6 Adult Thoracic-Abdominal Ganglion    3.6 Adult Crop    2.5 Adult Midgut    13.7 Adult Hindgut    3.9 Adult Malpighian Tubules    6.4 Adult Fat Body    14.5 Adult Salivary Gland    2.4 Adult Heart    10.225 Adult VirginFemale Spermatheca    21.9 Adult InseminatedFemale Spermatheca    5.2 Adult Ovary    11.2 Adult Testis    5.1 Adult Male Accessory Gland    4.7 Adult Carcass    6.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    4.95 Larval Midgut    5.8 Larval Hindgut    6.5 Larval Malpighian Tubules    4.2 Larval Fat Body    7.4 Larval Salivary Gland    5.6 Larval Trachea    3.35 Larval Carcass    8 Adult Head    4.9 Adult Eye    4.15 Adult Brain    2.6 Adult Thoracic-Abdominal Ganglion    3.6 Adult Crop    2.5 Adult Midgut    13.7 Adult Hindgut    3.9 Adult Malpighian Tubules    6.4 Adult Fat Body    14.5 Adult Salivary Gland    2.4 Adult Heart    10.225 Adult VirginFemale Spermatheca    21.9 Adult InseminatedFemale Spermatheca    5.2 Adult Ovary    11.2 Adult Testis    5.1 Adult Male Accessory Gland    4.7 Adult Carcass    6.5 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    4.9   NA  Eye    4.15   NA  Brain    2.6   4.95  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    3.6   NA  Crop    2.5   5.8  Midgut    13.7   6.5  Hindgut    3.9   4.2  Malpighian Tubules    6.4   7.4  Fat Body    no informative data   5.6  Salivary Gland    2.4   NA  Heart    no informative data   3.35  Trachea    NA   NA  VirginFemale Spermatheca    21.9   NA  InseminatedFemale Spermatheca    5.2   NA  Ovary    11.2   NA  Testis    5.1   NA  Male Accessory Gland    4.7   8  Carcass    6.5 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    3 central nervous system, larvae L3    0 central nervous system, pupae P8    1 head, virgin 1-day female    2 head, virgin 4-day female    2 head, virgin 20-day female    2 head, mated 1-day female    0 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    4 head, mated 4-day male    4 head, mated 20-day male    6 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    38 digestive system, 4-day adult    16 digestive system, 20-day adult    20 fat body, larvae L3 wandering    2 fat body, white prepupae    2 fat body, pupae P8    0 carcass, larvae L3 wandering    0 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    3 ovary, virgin 4-day female    23 ovary, mated 4-day female    16 testis, mated 4-day male    47 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    3 central nervous system, larvae L3    0 central nervous system, pupae P8    1 head, virgin 1-day female    2 head, virgin 4-day female    2 head, virgin 20-day female    2 head, mated 1-day female    0 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    4 head, mated 4-day male    4 head, mated 20-day male    6 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    1 digestive system, 1-day adult  (38) digestive system, 4-day adult    16 digestive system, 20-day adult    20 fat body, larvae L3 wandering    2 fat body, white prepupae    2 fat body, pupae P8    0 carcass, larvae L3 wandering    0 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    3 ovary, virgin 4-day female    23 ovary, mated 4-day female    16 testis, mated 4-day male  (47) accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    3 central nervous system, larvae L3    0 central nervous system, pupae P8    1 head, virgin 1-day female    2 head, virgin 4-day female    2 head, virgin 20-day female    2 head, mated 1-day female    0 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    4 head, mated 4-day male    4 head, mated 20-day male    6 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    38 digestive system, 4-day adult    16 digestive system, 20-day adult    20 fat body, larvae L3 wandering    2 fat body, white prepupae    2 fat body, pupae P8    0 carcass, larvae L3 wandering    0 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    3 ovary, virgin 4-day female    23 ovary, mated 4-day female    16 testis, mated 4-day male    47 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    3 central nervous system, larvae L3    0 central nervous system, pupae P8    1 head, virgin 1-day female    2 head, virgin 4-day female    2 head, virgin 20-day female    2 head, mated 1-day female    0 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    4 head, mated 4-day male    4 head, mated 20-day male    6 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    38 digestive system, 4-day adult    16 digestive system, 20-day adult    20 fat body, larvae L3 wandering    2 fat body, white prepupae    2 fat body, pupae P8    0 carcass, larvae L3 wandering    0 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    3 ovary, virgin 4-day female    23 ovary, mated 4-day female    16 testis, mated 4-day male    47 accessory gland, mated 4-day male    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    3 central nervous system, larvae L3    0 central nervous system, pupae P8    1 head, virgin 1-day female    2 head, virgin 4-day female    2 head, virgin 20-day female    2 head, mated 1-day female    0 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    4 head, mated 4-day male    4 head, mated 20-day male    6 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    38 digestive system, 4-day adult    16 digestive system, 20-day adult    20 fat body, larvae L3 wandering    2 fat body, white prepupae    2 fat body, pupae P8    0 carcass, larvae L3 wandering    0 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    3 ovary, virgin 4-day female    23 ovary, mated 4-day female    16 testis, mated 4-day male    47 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    3 central nervous system, larvae L3    0 central nervous system, pupae P8    1 head, virgin 1-day female    2 head, virgin 4-day female    2 head, virgin 20-day female    2 head, mated 1-day female    0 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    4 head, mated 4-day male    4 head, mated 20-day male    6 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    1 digestive system, 1-day adult  (38) digestive system, 4-day adult    16 digestive system, 20-day adult    20 fat body, larvae L3 wandering    2 fat body, white prepupae    2 fat body, pupae P8    0 carcass, larvae L3 wandering    0 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    3 ovary, virgin 4-day female    23 ovary, mated 4-day female    16 testis, mated 4-day male  (47) accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    3 central nervous system, larvae L3    0 central nervous system, pupae P8    1 head, virgin 1-day female    2 head, virgin 4-day female    2 head, virgin 20-day female    2 head, mated 1-day female    0 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    4 head, mated 4-day male    4 head, mated 20-day male    6 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    38 digestive system, 4-day adult    16 digestive system, 20-day adult    20 fat body, larvae L3 wandering    2 fat body, white prepupae    2 fat body, pupae P8    0 carcass, larvae L3 wandering    0 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    3 ovary, virgin 4-day female    23 ovary, mated 4-day female    16 testis, mated 4-day male    47 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    3 central nervous system, larvae L3    0 central nervous system, pupae P8    1 head, virgin 1-day female    2 head, virgin 4-day female    2 head, virgin 20-day female    2 head, mated 1-day female    0 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    4 head, mated 4-day male    4 head, mated 20-day male    6 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    38 digestive system, 4-day adult    16 digestive system, 20-day adult    20 fat body, larvae L3 wandering    2 fat body, white prepupae    2 fat body, pupae P8    0 carcass, larvae L3 wandering    0 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    3 ovary, virgin 4-day female    23 ovary, mated 4-day female    16 testis, mated 4-day male    47 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-06 and 18-24 hour embryonic stages, at stages throughout the larval period, in stages of adults of both sexes. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    35 embryo 02-04hr    11 embryo 04-06hr    7 embryo 06-08hr    6 embryo 08-10hr    11 embryo 10-12hr    14 embryo 12-14hr    8 embryo 14-16hr    13 embryo 16-18hr    18 embryo 18-20hr    21 embryo 20-22hr    27 embryo 22-24hr    34 larva L1    22 larva L2    37 larva L3 12hr old    22 larva L3 puffstage 1-2    19 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    5 white prepupae new    4 white prepupae 12hr    9 white prepupae 24hr    9 pupae 2d postWPP    8 pupae 3d postWPP    4 pupae 4d postWPP    14 adult male 01day    18 adult male 05day    19 adult male 30day    24 adult female 01day    17 adult female 05day    28 adult female 30day    27 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (35) embryo 02-04hr    11 embryo 04-06hr    7 embryo 06-08hr    6 embryo 08-10hr    11 embryo 10-12hr    14 embryo 12-14hr    8 embryo 14-16hr    13 embryo 16-18hr    18 embryo 18-20hr    21 embryo 20-22hr  (27) embryo 22-24hr  (34) larva L1    22 larva L2  (37) larva L3 12hr old    22 larva L3 puffstage 1-2    19 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    5 white prepupae new    4 white prepupae 12hr    9 white prepupae 24hr    9 pupae 2d postWPP    8 pupae 3d postWPP    4 pupae 4d postWPP    14 adult male 01day    18 adult male 05day    19 adult male 30day    24 adult female 01day    17 adult female 05day  (28) adult female 30day  (27) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    35 embryo 02-04hr    11 embryo 04-06hr    7 embryo 06-08hr    6 embryo 08-10hr    11 embryo 10-12hr    14 embryo 12-14hr    8 embryo 14-16hr    13 embryo 16-18hr    18 embryo 18-20hr    21 embryo 20-22hr    27 embryo 22-24hr    34 larva L1    22 larva L2    37 larva L3 12hr old    22 larva L3 puffstage 1-2    19 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    5 white prepupae new    4 white prepupae 12hr    9 white prepupae 24hr    9 pupae 2d postWPP    8 pupae 3d postWPP    4 pupae 4d postWPP    14 adult male 01day    18 adult male 05day    19 adult male 30day    24 adult female 01day    17 adult female 05day    28 adult female 30day    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    35 embryo 02-04hr    11 embryo 04-06hr    7 embryo 06-08hr    6 embryo 08-10hr    11 embryo 10-12hr    14 embryo 12-14hr    8 embryo 14-16hr    13 embryo 16-18hr    18 embryo 18-20hr    21 embryo 20-22hr    27 embryo 22-24hr    34 larva L1    22 larva L2    37 larva L3 12hr old    22 larva L3 puffstage 1-2    19 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    5 white prepupae new    4 white prepupae 12hr    9 white prepupae 24hr    9 pupae 2d postWPP    8 pupae 3d postWPP    4 pupae 4d postWPP    14 adult male 01day    18 adult male 05day    19 adult male 30day    24 adult female 01day    17 adult female 05day    28 adult female 30day    27 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    35 embryo 02-04hr    11 embryo 04-06hr    7 embryo 06-08hr    6 embryo 08-10hr    11 embryo 10-12hr    14 embryo 12-14hr    8 embryo 14-16hr    13 embryo 16-18hr    18 embryo 18-20hr    21 embryo 20-22hr    27 embryo 22-24hr    34 larva L1    22 larva L2    37 larva L3 12hr old    22 larva L3 puffstage 1-2    19 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    5 white prepupae new    4 white prepupae 12hr    9 white prepupae 24hr    9 pupae 2d postWPP    8 pupae 3d postWPP    4 pupae 4d postWPP    14 adult male 01day    18 adult male 05day    19 adult male 30day    24 adult female 01day    17 adult female 05day    28 adult female 30day    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (35) embryo 02-04hr    11 embryo 04-06hr    7 embryo 06-08hr    6 embryo 08-10hr    11 embryo 10-12hr    14 embryo 12-14hr    8 embryo 14-16hr    13 embryo 16-18hr    18 embryo 18-20hr    21 embryo 20-22hr  27 embryo 22-24hr  (34) larva L1    22 larva L2  (37) larva L3 12hr old    22 larva L3 puffstage 1-2    19 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    5 white prepupae new    4 white prepupae 12hr    9 white prepupae 24hr    9 pupae 2d postWPP    8 pupae 3d postWPP    4 pupae 4d postWPP    14 adult male 01day    18 adult male 05day    19 adult male 30day    24 adult female 01day    17 adult female 05day  28 adult female 30day  27 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    35 embryo 02-04hr    11 embryo 04-06hr    7 embryo 06-08hr    6 embryo 08-10hr    11 embryo 10-12hr    14 embryo 12-14hr    8 embryo 14-16hr    13 embryo 16-18hr    18 embryo 18-20hr    21 embryo 20-22hr    27 embryo 22-24hr    34 larva L1    22 larva L2    37 larva L3 12hr old    22 larva L3 puffstage 1-2    19 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    5 white prepupae new    4 white prepupae 12hr    9 white prepupae 24hr    9 pupae 2d postWPP    8 pupae 3d postWPP    4 pupae 4d postWPP    14 adult male 01day    18 adult male 05day    19 adult male 30day    24 adult female 01day    17 adult female 05day    28 adult female 30day    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    35 embryo 02-04hr    11 embryo 04-06hr    7 embryo 06-08hr    6 embryo 08-10hr    11 embryo 10-12hr    14 embryo 12-14hr    8 embryo 14-16hr    13 embryo 16-18hr    18 embryo 18-20hr    21 embryo 20-22hr    27 embryo 22-24hr    34 larva L1    22 larva L2    37 larva L3 12hr old    22 larva L3 puffstage 1-2    19 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    5 white prepupae new    4 white prepupae 12hr    9 white prepupae 24hr    9 pupae 2d postWPP    8 pupae 3d postWPP    4 pupae 4d postWPP    14 adult male 01day    18 adult male 05day    19 adult male 30day    24 adult female 01day    17 adult female 05day    28 adult female 30day    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    71 Schneider line 2 Sg4    5 embryonic 1182-4H    18 embryonic GM2    65 embryonic Kc167    102 embryonic S1    57 embryonic S3    18 leg disc CME L1    4 wing disc CME-W2    8 wing disc ML-DmD8    14 wing disc ML-DmD9    21 wing disc ML-DmD16-c3    2 wing disc ML-DmD21    14 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    27 antennal disc ML-DmD20-c5    36 mixed discs ML-DmD4-c1    9 CNS ML-DmBG1-c1    28 CNS ML-DmBG2-c2    121 tumorous blood cells mbn2    157 ovary fGS/OSS    7 ovary OSC    3 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (71) Schneider line 2 Sg4    5 embryonic 1182-4H    18 embryonic GM2  (65) embryonic Kc167  (102) embryonic S1  (57) embryonic S3    18 leg disc CME L1    4 wing disc CME-W2    8 wing disc ML-DmD8    14 wing disc ML-DmD9    21 wing disc ML-DmD16-c3    2 wing disc ML-DmD21    14 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11  (27) antennal disc ML-DmD20-c5  (36) mixed discs ML-DmD4-c1    9 CNS ML-DmBG1-c1  (28) CNS ML-DmBG2-c2  (121) tumorous blood cells mbn2  (157) ovary fGS/OSS    7 ovary OSC    3 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    71 Schneider line 2 Sg4    5 embryonic 1182-4H    18 embryonic GM2    65 embryonic Kc167  102 embryonic S1    57 embryonic S3    18 leg disc CME L1    4 wing disc CME-W2    8 wing disc ML-DmD8    14 wing disc ML-DmD9    21 wing disc ML-DmD16-c3    2 wing disc ML-DmD21    14 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    27 antennal disc ML-DmD20-c5    36 mixed discs ML-DmD4-c1    9 CNS ML-DmBG1-c1    28 CNS ML-DmBG2-c2  (121) tumorous blood cells mbn2  (157) ovary fGS/OSS    7 ovary OSC    3 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    71 Schneider line 2 Sg4    5 embryonic 1182-4H    18 embryonic GM2    65 embryonic Kc167    102 embryonic S1    57 embryonic S3    18 leg disc CME L1    4 wing disc CME-W2    8 wing disc ML-DmD8    14 wing disc ML-DmD9    21 wing disc ML-DmD16-c3    2 wing disc ML-DmD21    14 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    27 antennal disc ML-DmD20-c5    36 mixed discs ML-DmD4-c1    9 CNS ML-DmBG1-c1    28 CNS ML-DmBG2-c2    121 tumorous blood cells mbn2    157 ovary fGS/OSS    7 ovary OSC    3 ovary OSS    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    71 Schneider line 2 Sg4    5 embryonic 1182-4H    18 embryonic GM2    65 embryonic Kc167    102 embryonic S1    57 embryonic S3    18 leg disc CME L1    4 wing disc CME-W2    8 wing disc ML-DmD8    14 wing disc ML-DmD9    21 wing disc ML-DmD16-c3    2 wing disc ML-DmD21    14 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    27 antennal disc ML-DmD20-c5    36 mixed discs ML-DmD4-c1    9 CNS ML-DmBG1-c1    28 CNS ML-DmBG2-c2    121 tumorous blood cells mbn2    157 ovary fGS/OSS    7 ovary OSC    3 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (71) Schneider line 2 Sg4    5 embryonic 1182-4H    18 embryonic GM2  (65) embryonic Kc167  (102) embryonic S1  (57) embryonic S3    18 leg disc CME L1    4 wing disc CME-W2    8 wing disc ML-DmD8    14 wing disc ML-DmD9    21 wing disc ML-DmD16-c3    2 wing disc ML-DmD21    14 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11  27 antennal disc ML-DmD20-c5  (36) mixed discs ML-DmD4-c1    9 CNS ML-DmBG1-c1  28 CNS ML-DmBG2-c2  (121) tumorous blood cells mbn2  (157) ovary fGS/OSS    7 ovary OSC    3 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    71 Schneider line 2 Sg4    5 embryonic 1182-4H    18 embryonic GM2    65 embryonic Kc167  102 embryonic S1    57 embryonic S3    18 leg disc CME L1    4 wing disc CME-W2    8 wing disc ML-DmD8    14 wing disc ML-DmD9    21 wing disc ML-DmD16-c3    2 wing disc ML-DmD21    14 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    27 antennal disc ML-DmD20-c5    36 mixed discs ML-DmD4-c1    9 CNS ML-DmBG1-c1    28 CNS ML-DmBG2-c2  121 tumorous blood cells mbn2  (157) ovary fGS/OSS    7 ovary OSC    3 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    71 Schneider line 2 Sg4    5 embryonic 1182-4H    18 embryonic GM2    65 embryonic Kc167    102 embryonic S1    57 embryonic S3    18 leg disc CME L1    4 wing disc CME-W2    8 wing disc ML-DmD8    14 wing disc ML-DmD9    21 wing disc ML-DmD16-c3    2 wing disc ML-DmD21    14 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    27 antennal disc ML-DmD20-c5    36 mixed discs ML-DmD4-c1    9 CNS ML-DmBG1-c1    28 CNS ML-DmBG2-c2    121 tumorous blood cells mbn2    157 ovary fGS/OSS    7 ovary OSC    3 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    4 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    23 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    8 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    25 Ethanol 5% 3 hrs, larvae L3    39 Ethanol 10% 3 hrs, larvae L3    20 Caffeine 1.5 mg/ml 4 hrs, larvae L3    14 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    11 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    59 Rotenone 2 μg 12 hrs, larvae L3    16 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    4 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    23 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    8 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    25 Ethanol 5% 3 hrs, larvae L3  (39) Ethanol 10% 3 hrs, larvae L3    20 Caffeine 1.5 mg/ml 4 hrs, larvae L3    14 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    11 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult  (59) Rotenone 2 μg 12 hrs, larvae L3    16 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    4 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    23 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    8 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    25 Ethanol 5% 3 hrs, larvae L3    39 Ethanol 10% 3 hrs, larvae L3    20 Caffeine 1.5 mg/ml 4 hrs, larvae L3    14 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    11 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    59 Rotenone 2 μg 12 hrs, larvae L3    16 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    4 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    23 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    8 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    25 Ethanol 5% 3 hrs, larvae L3    39 Ethanol 10% 3 hrs, larvae L3    20 Caffeine 1.5 mg/ml 4 hrs, larvae L3    14 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    11 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    59 Rotenone 2 μg 12 hrs, larvae L3    16 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    4 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    23 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    8 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    25 Ethanol 5% 3 hrs, larvae L3    39 Ethanol 10% 3 hrs, larvae L3    20 Caffeine 1.5 mg/ml 4 hrs, larvae L3    14 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    11 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    59 Rotenone 2 μg 12 hrs, larvae L3    16 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    4 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    23 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    8 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    25 Ethanol 5% 3 hrs, larvae L3  (39) Ethanol 10% 3 hrs, larvae L3    20 Caffeine 1.5 mg/ml 4 hrs, larvae L3    14 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    11 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult  (59) Rotenone 2 μg 12 hrs, larvae L3    16 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    4 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    23 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    8 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    25 Ethanol 5% 3 hrs, larvae L3    39 Ethanol 10% 3 hrs, larvae L3    20 Caffeine 1.5 mg/ml 4 hrs, larvae L3    14 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    11 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    59 Rotenone 2 μg 12 hrs, larvae L3    16 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    4 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    23 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    8 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    25 Ethanol 5% 3 hrs, larvae L3    39 Ethanol 10% 3 hrs, larvae L3    20 Caffeine 1.5 mg/ml 4 hrs, larvae L3    14 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    11 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    59 Rotenone 2 μg 12 hrs, larvae L3    16 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_20
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0063492
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable, with Scer\GAL4pnr-MD237 GstE8GD14799 Phenotype manifest in Allele
Classical Alleles ( 0 )
For All Classical Alleles Show
Allele of GstE8 Class Mutagen Stocks Known lesion
Alleles Carried on Transgenic Constructs ( 2 )
For All Alleles Carried on Transgenic Constructs Show
Allele of GstE8 Class Mutagen Stocks Known lesion GstE8GD14799 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes GstE8KK104725 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD14799} NA P{KK104725} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{lacW}l(2)SH1140SH1140 No
Gene Ontology: Function, Process & Cellular Component ( 3 unique terms )
Terms Based on Experimental Evidence ( 0 terms )
Terms Based on Predictions or Assertions ( 3 terms )
Molecular Function
CV term Evidence References
glutathione transferase activity inferred from sequence or structural similarity with GstE1 (Curators, 2008--)
Biological Process
CV term Evidence References
glutathione metabolic process inferred from sequence or structural similarity with GstE1 (Curators, 2008--)
Cellular Component
CV term Evidence References
cytoplasm inferred from sequence or structural similarity with rat Gsto1 (Curators, 2008--)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
GstE8 - CG12123 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) GstE8 - CG14109 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) GstE8 - CG14262 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) GstE8 - CG3358 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) GstE8 - CG5660 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) GstE8 - CG5805 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) GstE8 - CG6506 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) GstE8 - CG8026 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) GstE8 - CG8950 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) GstE8 - CG9596 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) GstE8 - CycA anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) GstE8 - Dic4 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) GstE8 - Hsp22 experimental knowledge based (Guruharsha et al., 2011) GstE8 - Hsp23 experimental knowledge based (Guruharsha et al., 2011) GstE8 - Hsp27 experimental knowledge based (Guruharsha et al., 2011) GstE8 - Hsp68 experimental knowledge based (Guruharsha et al., 2011) GstE8 - Hsp70Aa experimental knowledge based (Guruharsha et al., 2011) GstE8 - Mcm3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) GstE8 - Pk17E anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) GstE8 - RagC-D anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) GstE8 - Socs44A anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) GstE8 - Tgt anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) GstE8 - morgue anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) GstE8 - ref(2)P experimental knowledge based (Guruharsha et al., 2011)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions GstE8 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 62792 DroID - A comprehensive database of gene and protein interactions. • FBgn0063492 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 37113
Orthologs
OrthoDB Orthologs (64) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6282MX Dipteran inclusive ortholog search No orthologs identified Insect inclusive ortholog search EOG6G79VV Arthropod inclusive ortholog search EOG6PNW0C Metazoa inclusive ortholog search EOG69D33R
Orthologs in Drosophila Species (EOG6282MX)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\GstE6     Drosophila melanogaster fruit fly  Dmel\GstE8     Drosophila melanogaster fruit fly  Dmel\GstE5     Drosophila melanogaster fruit fly  Dmel\GstE7     Drosophila simulans   Dsim\GD11377 Y Y Drosophila simulans   Dsim\GD11375   Y Drosophila simulans   Dsim\GD11374   Y Drosophila simulans   Dsim\GD11376   Y Drosophila sechellia   Dsec\GM21881   Y Drosophila sechellia   Dsec\GM21880   Y Drosophila sechellia   Dsec\GM21882 Y Y Drosophila erecta   Dere\GG21889   Y Drosophila erecta   Dere\GG21888   Y Drosophila erecta   Dere\GG21887   Y Drosophila erecta   Dere\GG21890 Y Y Drosophila yakuba   Dyak\GE11962   Y Drosophila yakuba   Dyak\GE11963   Y Drosophila yakuba   Dyak\GE11965 Y Y Drosophila yakuba   Dyak\GE11964   Y Drosophila ananassae   Dana\GF12173   Y Drosophila ananassae   Dana\GF12172   Y Drosophila ananassae   Dana\GF12171   Y Drosophila ananassae   Dana\GF12174   Y Drosophila pseudoobscura pseudoobscura   Dpse\GA14545   Y Drosophila persimilis   Dper\GL17773   Y Drosophila willistoni   Dwil\GK22990   Y Drosophila willistoni   Dwil\GK22989   Y Drosophila willistoni   Dwil\GK22991   Y Drosophila virilis   Dvir\GJ19892   Y Drosophila virilis   Dvir\GJ19893   Y Drosophila mojavensis   Dmoj\GI20123   Y Drosophila mojavensis   Dmoj\GI20122   Y Drosophila grimshawi   Dgri\GH21669   Y
Orthologs in non-Drosophila Dipterans (None identified)
No non-Drosophilid orthologies identified
Orthologs in non-Dipteran Insects (EOG6G79VV)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Bombyx mori Silkmoth  Bmor\BGIBMGA002279 Y Bombyx mori Silkmoth  Bmor\BGIBMGA010094 Y Bombyx mori Silkmoth  Bmor\BGIBMGA010093 Y Bombyx mori Silkmoth  Bmor\BGIBMGA009951 Y Tribolium castaneum Red flour beetle  Tcas\TC004448 Y Tribolium castaneum Red flour beetle  Tcas\TC004444 Y Tribolium castaneum Red flour beetle  Tcas\TC003346 Y Tribolium castaneum Red flour beetle  Tcas\TC004942 Y Tribolium castaneum Red flour beetle  Tcas\TC004940 Y Tribolium castaneum Red flour beetle  Tcas\TC003104 Y Tribolium castaneum Red flour beetle  Tcas\TC003345 Y Tribolium castaneum Red flour beetle  Tcas\TC004446 Y Tribolium castaneum Red flour beetle  Tcas\TC003348 Y Tribolium castaneum Red flour beetle  Tcas\TC004442 Y Tribolium castaneum Red flour beetle  Tcas\TC003103 Y Tribolium castaneum Red flour beetle  Tcas\TC004447 Y Tribolium castaneum Red flour beetle  Tcas\TC004941 Y Tribolium castaneum Red flour beetle  Tcas\TC004443 Y Tribolium castaneum Red flour beetle  Tcas\TC004445 Y Tribolium castaneum Red flour beetle  Tcas\TC004449 Y
Orthologs in non-Insect Arthropods (EOG6PNW0C)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Ixodes scapularis Deer tick  Isca\ISCW007012 Y Ixodes scapularis Deer tick  Isca\ISCW015490 Y Ixodes scapularis Deer tick  Isca\ISCW005805 Y Ixodes scapularis Deer tick  Isca\ISCW008097 Y Ixodes scapularis Deer tick  Isca\ISCW005804 Y
Orthologs in non-Arthropod Metazoa (EOG69D33R)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_027539gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_006492gn Y Mus musculus House mouse  Mmus\Gstt2 Y Rattus norvegicus Norway rat  Rnor\Gstt2 Y Homo sapiens Human  Hsap\GSTT2 Y Homo sapiens Human  Hsap\GSTT2B Y
Human Orthologs (2)
Gene OMIM HGNC Hsap\GSTT2     Hsap\GSTT2B
AAA Orthologs (4) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD11377 Drosophila sechellia Dsec\GM21882 Drosophila erecta Dere\GG21890 Drosophila yakuba Dyak\GE11965
Stocks & Reagents
Stocks Listed in FlyBase ( 3 )
VDRC	v100516 P{KK104725}VIE-260B v36534 w1118; P{GD14799}v36534 v36535 w1118; P{GD14799}v36535
Genomic Clones ( 1 )
	BACR48A20 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 12 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GM08326
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones	61411 61796 61862 148623 214371.3 562167 EK185152 EK185153 EK253948 EN13838 EP05734
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0063492 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37113
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Gst3-2 CG17533 (FlyBase, 1996-)
Source for database merge of	Source for merge of: Gst3-2 BcDNA:GM08326 (FlyBase, 1996-)
Additional comments	Source for merge of Gst3-2 BcDNA:GM08326 was a shared cDNA (date:030728). (FlyBase, 1996-) Source for identity of Gst3-2 CG17533 was sequence comparison (date:020320). (FlyBase, 1996-)
Other Comments
	The form of GstE8 cloned from S2 cells is truncated and lacks part of the active site. (Saisawang et al., 2012)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_137486 RefSeq (Proteins) • NP_611330 DDBJ / EMBL / GenBank • AA696749 AAF57694 AAL68213 AJ437579 AW941310 AY075363 CAD26834 Entrez Gene - A searchable database of RefSeq genes. • 37113 UniProtKB/TrEMBL • A1ZB73 Q8T0X2 Q8T8R2
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Glutathione S-transferase, N-terminal (IPR004045) Glutathione S-transferase, C-terminal (IPR004046) Glutathione S-transferase, C-terminal-like (IPR010987) Thioredoxin-like fold (IPR012336) Glutathione S-transferase/chloride channel, C-terminal (IPR017933)
Linkouts
	BioGRID - A database of protein and genetic interactions • 62792 DroID - A comprehensive database of gene and protein interactions. • FBgn0063492 DRSC - Results from RNAi screens. • FBgn0063492 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0063492 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0063492 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37113 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 37113 modMine - Data generated by the modENCODE project. • FBgn0063492
Synonyms & Secondary IDs ( 9 )
Reported As
Symbol Synonym	BcDNA:GM08326   CG17533 (van Steensel et al., 2003, Ranson et al., 2001, Keleman et al., 2009.8.5, Marco et al., 2004) DmGSTE8 (Sawicki et al., 2003, Zimniak and Benes, 2002.7.15) GST3-2 (Hovemann, 2002.2.28) Gst3-2 (Giot et al., 2003, ) GstE8 (Gruenewald et al., 2009, Drnevich et al., 2004, Kim et al., 2005, Neal et al., 2006, Awofala et al., 2012) GSTE8 (Low et al., 2007, Li et al., 2008) gste8 (Saisawang et al., 2012)
Name Synonym	Glutathione S transferase E8 (Zimniak and Benes, 2002.7.15)
Secondary FlyBase IDs
	FBgn0034342 FBgn0061421
References ( 29 )
Generate a list of	All references relating to this gene ( 29 )
List References by type	Research paper  ( 14 ) Supplementary material  ( 5 ) Personal communication to FlyBase  ( 5 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 1 ) Curated genome annotation  ( 1 ) Thesis  ( 1 )
Recent research papers ( 3 )
	Awofala et al., 2012, Funct. Integr. Genomics 12(2): 305--315 Functional roles for redox genes in ethanol sensitivity in Drosophila. [FBrf0218408] Saisawang et al., 2012, Biochem. J. 442(1): 181--190 A preliminary characterization of the cytosolic glutathione transferase proteome from Drosophila melanogaster. [FBrf0217315] Weber et al., 2012, PLoS ONE 7(4): e34745 Genome-Wide Association Analysis of Oxidative Stress Resistance in Drosophila melanogaster. [FBrf0218073] Show all research papers ...