Gene Dmel\GstE4

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General Information

Symbol

Dmel\GstE4

Species

D. melanogaster

Name

Glutathione S transferase E4

Annotation symbol

CG17525

Feature type

protein_coding_gene

FlyBase ID

FBgn0063496

Gene Model Status

Current

Stock availability

2 publicly available

Also Known As

GSTE4, DmGSTE4

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

55C7-55C7

Sequence location

2R:14,290,753..14,291,591 [+]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

The gene Glutathione S transferase E4 is referred to in FlyBase by the symbol Dmel\GstE4 (CG17525, FBgn0063496). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: glutathione transferase activity. There is experimental evidence that it is involved in the biological process: glutathione metabolic process. 2 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Glutathione S-transferase, C-terminal; Glutathione S-transferase, C-terminal-like; Glutathione S-transferase, N-terminal; Glutathione S-transferase/chloride channel, C-terminal; Thioredoxin-like fold. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of extremely low expression. Peak expression observed within 18-24 hour embryonic stages, during late pupal stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult head. Comments on Affy2 ProbeSet: ProbeSet 1632539_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of GstE4. Gene sequence location is 2R:14290753..14291591.

User Contributed Data

Beta version, feedback welcome

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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FB2013_03

Controlled Vocabulary Terms

cytoplasm

FB2013_02

All updates

Click here to see a list of all updates to this record from FB2010_08 and on.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

55C7-55C7

Limits computationally determined from genome sequence between P{PZ}sbb⁰⁴⁵²⁵&P{EP}EP1081^EP1081 and P{lacW}l(2)08770^k04808

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\GstE4 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Gene model reviewed during 5.43

Gene model reviewed during 5.50

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

GstE4-RA

FBtr0086672

NM_137482

839

222

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

GstE4-PA

FBpp0085853

25.3

222

4.52

NP_611326

AAF57698

Additional Polypeptide Data & Comments

Reported size (kDa)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Glutathione S-transferase, N-terminal (IPR004045)

Glutathione S-transferase, C-terminal (IPR004046)

Glutathione S-transferase, C-terminal-like (IPR010987)

Thioredoxin-like fold (IPR012336)

Glutathione S-transferase/chloride channel, C-terminal (IPR017933)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

A1ZB69

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult head.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		2.7
Larval Midgut		3.3
Larval Hindgut		3.6
Larval Malpighian Tubules		0.7
Larval Fat Body		2.9
Larval Salivary Gland		5.7
Larval Trachea		0.625
Larval Carcass		3.8
Adult Head		524.5
Adult Eye		78.625
Adult Brain		7
Adult Thoracic-Abdominal Ganglion		2.9
Adult Crop		2.6
Adult Midgut		5.8
Adult Hindgut		6.2
Adult Malpighian Tubules		2.4
Adult Fat Body		2.7
Adult Salivary Gland		6
Adult Heart		2.825
Adult VirginFemale Spermatheca		3.2
Adult InseminatedFemale Spermatheca		1.2
Adult Ovary		2.2
Adult Testis		1.1
Adult Male Accessory Gland		7.3
Adult Carcass		7.6
Expression Level Scale		None Low Moderate High

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		2.7
Larval Midgut		3.3
Larval Hindgut		3.6
Larval Malpighian Tubules		0.7
Larval Fat Body		2.9
Larval Salivary Gland		5.7
Larval Trachea		0.625
Larval Carcass		3.8
Adult Head		524.5
Adult Eye		78.625
Adult Brain		7
Adult Thoracic-Abdominal Ganglion		2.9
Adult Crop		2.6
Adult Midgut		5.8
Adult Hindgut		6.2
Adult Malpighian Tubules		2.4
Adult Fat Body		2.7
Adult Salivary Gland		6
Adult Heart		2.825
Adult VirginFemale Spermatheca		3.2
Adult InseminatedFemale Spermatheca		1.2
Adult Ovary		2.2
Adult Testis		1.1
Adult Male Accessory Gland		7.3
Adult Carcass		7.6
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		2.7
Larval Midgut		3.3
Larval Hindgut		3.6
Larval Malpighian Tubules		0.7
Larval Fat Body		2.9
Larval Salivary Gland		5.7
Larval Trachea		0.625
Larval Carcass		3.8
Adult Head		524.5
Adult Eye		78.625
Adult Brain		7
Adult Thoracic-Abdominal Ganglion		2.9
Adult Crop		2.6
Adult Midgut		5.8
Adult Hindgut		6.2
Adult Malpighian Tubules		2.4
Adult Fat Body		2.7
Adult Salivary Gland		6
Adult Heart		2.825
Adult VirginFemale Spermatheca		3.2
Adult InseminatedFemale Spermatheca		1.2
Adult Ovary		2.2
Adult Testis		1.1
Adult Male Accessory Gland		7.3
Adult Carcass		7.6
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		2.7
Larval Midgut		3.3
Larval Hindgut		3.6
Larval Malpighian Tubules		0.7
Larval Fat Body		2.9
Larval Salivary Gland		5.7
Larval Trachea		0.625
Larval Carcass		3.8
Adult Head		524.5
Adult Eye		78.625
Adult Brain		7
Adult Thoracic-Abdominal Ganglion		2.9
Adult Crop		2.6
Adult Midgut		5.8
Adult Hindgut		6.2
Adult Malpighian Tubules		2.4
Adult Fat Body		2.7
Adult Salivary Gland		6
Adult Heart		2.825
Adult VirginFemale Spermatheca		3.2
Adult InseminatedFemale Spermatheca		1.2
Adult Ovary		2.2
Adult Testis		1.1
Adult Male Accessory Gland		7.3
Adult Carcass		7.6
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		2.7
Larval Midgut		3.3
Larval Hindgut		3.6
Larval Malpighian Tubules		0.7
Larval Fat Body		2.9
Larval Salivary Gland		5.7
Larval Trachea		0.625
Larval Carcass		3.8
Adult Head		524.5
Adult Eye		78.625
Adult Brain		7
Adult Thoracic-Abdominal Ganglion		2.9
Adult Crop		2.6
Adult Midgut		5.8
Adult Hindgut		6.2
Adult Malpighian Tubules		2.4
Adult Fat Body		2.7
Adult Salivary Gland		6
Adult Heart		2.825
Adult VirginFemale Spermatheca		3.2
Adult InseminatedFemale Spermatheca		1.2
Adult Ovary		2.2
Adult Testis		1.1
Adult Male Accessory Gland		7.3
Adult Carcass		7.6
Expression Level Scale		None Low Moderate High

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		2.7
Larval Midgut		3.3
Larval Hindgut		3.6
Larval Malpighian Tubules		0.7
Larval Fat Body		2.9
Larval Salivary Gland		5.7
Larval Trachea		0.625
Larval Carcass		3.8
Adult Head		524.5
Adult Eye		78.625
Adult Brain		7
Adult Thoracic-Abdominal Ganglion		2.9
Adult Crop		2.6
Adult Midgut		5.8
Adult Hindgut		6.2
Adult Malpighian Tubules		2.4
Adult Fat Body		2.7
Adult Salivary Gland		6
Adult Heart		2.825
Adult VirginFemale Spermatheca		3.2
Adult InseminatedFemale Spermatheca		1.2
Adult Ovary		2.2
Adult Testis		1.1
Adult Male Accessory Gland		7.3
Adult Carcass		7.6
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		2.7
Larval Midgut		3.3
Larval Hindgut		3.6
Larval Malpighian Tubules		0.7
Larval Fat Body		2.9
Larval Salivary Gland		5.7
Larval Trachea		0.625
Larval Carcass		3.8
Adult Head		524.5
Adult Eye		78.625
Adult Brain		7
Adult Thoracic-Abdominal Ganglion		2.9
Adult Crop		2.6
Adult Midgut		5.8
Adult Hindgut		6.2
Adult Malpighian Tubules		2.4
Adult Fat Body		2.7
Adult Salivary Gland		6
Adult Heart		2.825
Adult VirginFemale Spermatheca		3.2
Adult InseminatedFemale Spermatheca		1.2
Adult Ovary		2.2
Adult Testis		1.1
Adult Male Accessory Gland		7.3
Adult Carcass		7.6
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		2.7
Larval Midgut		3.3
Larval Hindgut		3.6
Larval Malpighian Tubules		0.7
Larval Fat Body		2.9
Larval Salivary Gland		5.7
Larval Trachea		0.625
Larval Carcass		3.8
Adult Head		524.5
Adult Eye		78.625
Adult Brain		7
Adult Thoracic-Abdominal Ganglion		2.9
Adult Crop		2.6
Adult Midgut		5.8
Adult Hindgut		6.2
Adult Malpighian Tubules		2.4
Adult Fat Body		2.7
Adult Salivary Gland		6
Adult Heart		2.825
Adult VirginFemale Spermatheca		3.2
Adult InseminatedFemale Spermatheca		1.2
Adult Ovary		2.2
Adult Testis		1.1
Adult Male Accessory Gland		7.3
Adult Carcass		7.6
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	524.5
NA	Eye	no informative data
NA	Brain	7
2.7	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	2.9
NA	Crop	2.6
3.3	Midgut	5.8
3.6	Hindgut	6.2
0.7	Malpighian Tubules	2.4
2.9	Fat Body	2.7
5.7	Salivary Gland	6
NA	Heart	2.825
0.625	Trachea	NA
NA	VirginFemale Spermatheca	3.2
NA	InseminatedFemale Spermatheca	1.2
NA	Ovary	2.2
NA	Testis	1.1
NA	Male Accessory Gland	7.3
3.8	Carcass	7.6

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		0
central nervous system, larvae L3		0
central nervous system, pupae P8		0
head, virgin 1-day female		1
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		0
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		0
digestive system, 1-day adult		0
digestive system, 4-day adult		0
digestive system, 20-day adult		0
fat body, larvae L3 wandering		0
fat body, white prepupae		0
fat body, pupae P8		0
carcass, larvae L3 wandering		0
carcass, 1-day adult		0
carcass, 4-day adult		0
carcass, 20-day adult		0
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		0
accessory gland, mated 4-day male		0
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		0
central nervous system, larvae L3		0
central nervous system, pupae P8		0
head, virgin 1-day female		1
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		0
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		0
digestive system, 1-day adult		0
digestive system, 4-day adult		0
digestive system, 20-day adult		0
fat body, larvae L3 wandering		0
fat body, white prepupae		0
fat body, pupae P8		0
carcass, larvae L3 wandering		0
carcass, 1-day adult		0
carcass, 4-day adult		0
carcass, 20-day adult		0
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		0
accessory gland, mated 4-day male		0
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		0
central nervous system, larvae L3		0
central nervous system, pupae P8		0
head, virgin 1-day female		1
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		0
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		0
digestive system, 1-day adult		0
digestive system, 4-day adult		0
digestive system, 20-day adult		0
fat body, larvae L3 wandering		0
fat body, white prepupae		0
fat body, pupae P8		0
carcass, larvae L3 wandering		0
carcass, 1-day adult		0
carcass, 4-day adult		0
carcass, 20-day adult		0
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		0
accessory gland, mated 4-day male		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		0
central nervous system, larvae L3		0
central nervous system, pupae P8		0
head, virgin 1-day female		1
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		0
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		0
digestive system, 1-day adult		0
digestive system, 4-day adult		0
digestive system, 20-day adult		0
fat body, larvae L3 wandering		0
fat body, white prepupae		0
fat body, pupae P8		0
carcass, larvae L3 wandering		0
carcass, 1-day adult		0
carcass, 4-day adult		0
carcass, 20-day adult		0
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		0
accessory gland, mated 4-day male		0
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		0
central nervous system, larvae L3		0
central nervous system, pupae P8		0
head, virgin 1-day female		1
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		0
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		0
digestive system, 1-day adult		0
digestive system, 4-day adult		0
digestive system, 20-day adult		0
fat body, larvae L3 wandering		0
fat body, white prepupae		0
fat body, pupae P8		0
carcass, larvae L3 wandering		0
carcass, 1-day adult		0
carcass, 4-day adult		0
carcass, 20-day adult		0
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		0
accessory gland, mated 4-day male		0
Expression Level Scale		Very low Low Moderate

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		0
central nervous system, larvae L3		0
central nervous system, pupae P8		0
head, virgin 1-day female		1
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		0
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		0
digestive system, 1-day adult		0
digestive system, 4-day adult		0
digestive system, 20-day adult		0
fat body, larvae L3 wandering		0
fat body, white prepupae		0
fat body, pupae P8		0
carcass, larvae L3 wandering		0
carcass, 1-day adult		0
carcass, 4-day adult		0
carcass, 20-day adult		0
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		0
accessory gland, mated 4-day male		0
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		0
central nervous system, larvae L3		0
central nervous system, pupae P8		0
head, virgin 1-day female		1
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		0
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		0
digestive system, 1-day adult		0
digestive system, 4-day adult		0
digestive system, 20-day adult		0
fat body, larvae L3 wandering		0
fat body, white prepupae		0
fat body, pupae P8		0
carcass, larvae L3 wandering		0
carcass, 1-day adult		0
carcass, 4-day adult		0
carcass, 20-day adult		0
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		0
accessory gland, mated 4-day male		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		0
central nervous system, larvae L3		0
central nervous system, pupae P8		0
head, virgin 1-day female		1
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		0
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		0
digestive system, 1-day adult		0
digestive system, 4-day adult		0
digestive system, 20-day adult		0
fat body, larvae L3 wandering		0
fat body, white prepupae		0
fat body, pupae P8		0
carcass, larvae L3 wandering		0
carcass, 1-day adult		0
carcass, 4-day adult		0
carcass, 20-day adult		0
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		0
accessory gland, mated 4-day male		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of extremely low expression. Peak expression observed within 18-24 hour embryonic stages, during late pupal stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		4
embryo 16-18hr		7
embryo 18-20hr		10
embryo 20-22hr		16
embryo 22-24hr		18
larva L1		18
larva L2		6
larva L3 12hr old		1
larva L3 puffstage 1-2		0
larva L3 puffstage 3-6		0
larva L3 puffstage 7-9		0
white prepupae new		1
white prepupae 12hr		0
white prepupae 24hr		0
pupae 2d postWPP		1
pupae 3d postWPP		1
pupae 4d postWPP		21
adult male 01day		11
adult male 05day		8
adult male 30day		15
adult female 01day		4
adult female 05day		2
adult female 30day		2
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		4
embryo 16-18hr		7
embryo 18-20hr		10
embryo 20-22hr		16
embryo 22-24hr		18
larva L1		18
larva L2		6
larva L3 12hr old		1
larva L3 puffstage 1-2		0
larva L3 puffstage 3-6		0
larva L3 puffstage 7-9		0
white prepupae new		1
white prepupae 12hr		0
white prepupae 24hr		0
pupae 2d postWPP		1
pupae 3d postWPP		1
pupae 4d postWPP		21
adult male 01day		11
adult male 05day		8
adult male 30day		15
adult female 01day		4
adult female 05day		2
adult female 30day		2
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		4
embryo 16-18hr		7
embryo 18-20hr		10
embryo 20-22hr		16
embryo 22-24hr		18
larva L1		18
larva L2		6
larva L3 12hr old		1
larva L3 puffstage 1-2		0
larva L3 puffstage 3-6		0
larva L3 puffstage 7-9		0
white prepupae new		1
white prepupae 12hr		0
white prepupae 24hr		0
pupae 2d postWPP		1
pupae 3d postWPP		1
pupae 4d postWPP		21
adult male 01day		11
adult male 05day		8
adult male 30day		15
adult female 01day		4
adult female 05day		2
adult female 30day		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		4
embryo 16-18hr		7
embryo 18-20hr		10
embryo 20-22hr		16
embryo 22-24hr		18
larva L1		18
larva L2		6
larva L3 12hr old		1
larva L3 puffstage 1-2		0
larva L3 puffstage 3-6		0
larva L3 puffstage 7-9		0
white prepupae new		1
white prepupae 12hr		0
white prepupae 24hr		0
pupae 2d postWPP		1
pupae 3d postWPP		1
pupae 4d postWPP		21
adult male 01day		11
adult male 05day		8
adult male 30day		15
adult female 01day		4
adult female 05day		2
adult female 30day		2
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		4
embryo 16-18hr		7
embryo 18-20hr		10
embryo 20-22hr		16
embryo 22-24hr		18
larva L1		18
larva L2		6
larva L3 12hr old		1
larva L3 puffstage 1-2		0
larva L3 puffstage 3-6		0
larva L3 puffstage 7-9		0
white prepupae new		1
white prepupae 12hr		0
white prepupae 24hr		0
pupae 2d postWPP		1
pupae 3d postWPP		1
pupae 4d postWPP		21
adult male 01day		11
adult male 05day		8
adult male 30day		15
adult female 01day		4
adult female 05day		2
adult female 30day		2
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		4
embryo 16-18hr		7
embryo 18-20hr		10
embryo 20-22hr		16
embryo 22-24hr		18
larva L1		18
larva L2		6
larva L3 12hr old		1
larva L3 puffstage 1-2		0
larva L3 puffstage 3-6		0
larva L3 puffstage 7-9		0
white prepupae new		1
white prepupae 12hr		0
white prepupae 24hr		0
pupae 2d postWPP		1
pupae 3d postWPP		1
pupae 4d postWPP		21
adult male 01day		11
adult male 05day		8
adult male 30day		15
adult female 01day		4
adult female 05day		2
adult female 30day		2
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		4
embryo 16-18hr		7
embryo 18-20hr		10
embryo 20-22hr		16
embryo 22-24hr		18
larva L1		18
larva L2		6
larva L3 12hr old		1
larva L3 puffstage 1-2		0
larva L3 puffstage 3-6		0
larva L3 puffstage 7-9		0
white prepupae new		1
white prepupae 12hr		0
white prepupae 24hr		0
pupae 2d postWPP		1
pupae 3d postWPP		1
pupae 4d postWPP		21
adult male 01day		11
adult male 05day		8
adult male 30day		15
adult female 01day		4
adult female 05day		2
adult female 30day		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		4
embryo 16-18hr		7
embryo 18-20hr		10
embryo 20-22hr		16
embryo 22-24hr		18
larva L1		18
larva L2		6
larva L3 12hr old		1
larva L3 puffstage 1-2		0
larva L3 puffstage 3-6		0
larva L3 puffstage 7-9		0
white prepupae new		1
white prepupae 12hr		0
white prepupae 24hr		0
pupae 2d postWPP		1
pupae 3d postWPP		1
pupae 4d postWPP		21
adult male 01day		11
adult male 05day		8
adult male 30day		15
adult female 01day		4
adult female 05day		2
adult female 30day		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		0
cold shock, 4-day adult		0
heat shock, 4-day adult		0
Cadmium 50 mM 6 hrs, larvae L3		0
Cadmium 50 mM 12 hrs, larvae L3		0
Cadmium 50 mM 48 hrs, 4-day adult		0
Cadmium 100 mM 48 hrs, 4-day adult		0
Copper 0.5 mM 12 hrs, larvae L3		0
Copper 15 mM 48 hrs, 4-day adult		2
Zinc 5 mM 12 hrs, larvae L3		0
Zinc 4.5 mM 48 hrs, 4-day adult		0
Ethanol 2.5% 3 hrs, larvae L3		0
Ethanol 5% 3 hrs, larvae L3		0
Ethanol 10% 3 hrs, larvae L3		0
Caffeine 1.5 mg/ml 4 hrs, larvae L3		0
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		0
Caffeine 25 mg/ml 48 hrs, 4-day adult		0
Paraquat 5 mM 48 hrs, 4-day adult		1
Paraquat 10 mM 48 hrs, 4-day adult		1
Rotenone 2 μg 12 hrs, larvae L3		0
Rotenone 8 μg 12 hrs, larvae L3		0
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		0
cold shock, 4-day adult		0
heat shock, 4-day adult		0
Cadmium 50 mM 6 hrs, larvae L3		0
Cadmium 50 mM 12 hrs, larvae L3		0
Cadmium 50 mM 48 hrs, 4-day adult		0
Cadmium 100 mM 48 hrs, 4-day adult		0
Copper 0.5 mM 12 hrs, larvae L3		0
Copper 15 mM 48 hrs, 4-day adult		2
Zinc 5 mM 12 hrs, larvae L3		0
Zinc 4.5 mM 48 hrs, 4-day adult		0
Ethanol 2.5% 3 hrs, larvae L3		0
Ethanol 5% 3 hrs, larvae L3		0
Ethanol 10% 3 hrs, larvae L3		0
Caffeine 1.5 mg/ml 4 hrs, larvae L3		0
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		0
Caffeine 25 mg/ml 48 hrs, 4-day adult		0
Paraquat 5 mM 48 hrs, 4-day adult		1
Paraquat 10 mM 48 hrs, 4-day adult		1
Rotenone 2 μg 12 hrs, larvae L3		0
Rotenone 8 μg 12 hrs, larvae L3		0
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		0
cold shock, 4-day adult		0
heat shock, 4-day adult		0
Cadmium 50 mM 6 hrs, larvae L3		0
Cadmium 50 mM 12 hrs, larvae L3		0
Cadmium 50 mM 48 hrs, 4-day adult		0
Cadmium 100 mM 48 hrs, 4-day adult		0
Copper 0.5 mM 12 hrs, larvae L3		0
Copper 15 mM 48 hrs, 4-day adult		2
Zinc 5 mM 12 hrs, larvae L3		0
Zinc 4.5 mM 48 hrs, 4-day adult		0
Ethanol 2.5% 3 hrs, larvae L3		0
Ethanol 5% 3 hrs, larvae L3		0
Ethanol 10% 3 hrs, larvae L3		0
Caffeine 1.5 mg/ml 4 hrs, larvae L3		0
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		0
Caffeine 25 mg/ml 48 hrs, 4-day adult		0
Paraquat 5 mM 48 hrs, 4-day adult		1
Paraquat 10 mM 48 hrs, 4-day adult		1
Rotenone 2 μg 12 hrs, larvae L3		0
Rotenone 8 μg 12 hrs, larvae L3		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		0
cold shock, 4-day adult		0
heat shock, 4-day adult		0
Cadmium 50 mM 6 hrs, larvae L3		0
Cadmium 50 mM 12 hrs, larvae L3		0
Cadmium 50 mM 48 hrs, 4-day adult		0
Cadmium 100 mM 48 hrs, 4-day adult		0
Copper 0.5 mM 12 hrs, larvae L3		0
Copper 15 mM 48 hrs, 4-day adult		2
Zinc 5 mM 12 hrs, larvae L3		0
Zinc 4.5 mM 48 hrs, 4-day adult		0
Ethanol 2.5% 3 hrs, larvae L3		0
Ethanol 5% 3 hrs, larvae L3		0
Ethanol 10% 3 hrs, larvae L3		0
Caffeine 1.5 mg/ml 4 hrs, larvae L3		0
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		0
Caffeine 25 mg/ml 48 hrs, 4-day adult		0
Paraquat 5 mM 48 hrs, 4-day adult		1
Paraquat 10 mM 48 hrs, 4-day adult		1
Rotenone 2 μg 12 hrs, larvae L3		0
Rotenone 8 μg 12 hrs, larvae L3		0
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		0
cold shock, 4-day adult		0
heat shock, 4-day adult		0
Cadmium 50 mM 6 hrs, larvae L3		0
Cadmium 50 mM 12 hrs, larvae L3		0
Cadmium 50 mM 48 hrs, 4-day adult		0
Cadmium 100 mM 48 hrs, 4-day adult		0
Copper 0.5 mM 12 hrs, larvae L3		0
Copper 15 mM 48 hrs, 4-day adult		2
Zinc 5 mM 12 hrs, larvae L3		0
Zinc 4.5 mM 48 hrs, 4-day adult		0
Ethanol 2.5% 3 hrs, larvae L3		0
Ethanol 5% 3 hrs, larvae L3		0
Ethanol 10% 3 hrs, larvae L3		0
Caffeine 1.5 mg/ml 4 hrs, larvae L3		0
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		0
Caffeine 25 mg/ml 48 hrs, 4-day adult		0
Paraquat 5 mM 48 hrs, 4-day adult		1
Paraquat 10 mM 48 hrs, 4-day adult		1
Rotenone 2 μg 12 hrs, larvae L3		0
Rotenone 8 μg 12 hrs, larvae L3		0
Expression Level Scale		Very low Low Moderate

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		0
cold shock, 4-day adult		0
heat shock, 4-day adult		0
Cadmium 50 mM 6 hrs, larvae L3		0
Cadmium 50 mM 12 hrs, larvae L3		0
Cadmium 50 mM 48 hrs, 4-day adult		0
Cadmium 100 mM 48 hrs, 4-day adult		0
Copper 0.5 mM 12 hrs, larvae L3		0
Copper 15 mM 48 hrs, 4-day adult		2
Zinc 5 mM 12 hrs, larvae L3		0
Zinc 4.5 mM 48 hrs, 4-day adult		0
Ethanol 2.5% 3 hrs, larvae L3		0
Ethanol 5% 3 hrs, larvae L3		0
Ethanol 10% 3 hrs, larvae L3		0
Caffeine 1.5 mg/ml 4 hrs, larvae L3		0
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		0
Caffeine 25 mg/ml 48 hrs, 4-day adult		0
Paraquat 5 mM 48 hrs, 4-day adult		1
Paraquat 10 mM 48 hrs, 4-day adult		1
Rotenone 2 μg 12 hrs, larvae L3		0
Rotenone 8 μg 12 hrs, larvae L3		0
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		0
cold shock, 4-day adult		0
heat shock, 4-day adult		0
Cadmium 50 mM 6 hrs, larvae L3		0
Cadmium 50 mM 12 hrs, larvae L3		0
Cadmium 50 mM 48 hrs, 4-day adult		0
Cadmium 100 mM 48 hrs, 4-day adult		0
Copper 0.5 mM 12 hrs, larvae L3		0
Copper 15 mM 48 hrs, 4-day adult		2
Zinc 5 mM 12 hrs, larvae L3		0
Zinc 4.5 mM 48 hrs, 4-day adult		0
Ethanol 2.5% 3 hrs, larvae L3		0
Ethanol 5% 3 hrs, larvae L3		0
Ethanol 10% 3 hrs, larvae L3		0
Caffeine 1.5 mg/ml 4 hrs, larvae L3		0
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		0
Caffeine 25 mg/ml 48 hrs, 4-day adult		0
Paraquat 5 mM 48 hrs, 4-day adult		1
Paraquat 10 mM 48 hrs, 4-day adult		1
Rotenone 2 μg 12 hrs, larvae L3		0
Rotenone 8 μg 12 hrs, larvae L3		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		0
cold shock, 4-day adult		0
heat shock, 4-day adult		0
Cadmium 50 mM 6 hrs, larvae L3		0
Cadmium 50 mM 12 hrs, larvae L3		0
Cadmium 50 mM 48 hrs, 4-day adult		0
Cadmium 100 mM 48 hrs, 4-day adult		0
Copper 0.5 mM 12 hrs, larvae L3		0
Copper 15 mM 48 hrs, 4-day adult		2
Zinc 5 mM 12 hrs, larvae L3		0
Zinc 4.5 mM 48 hrs, 4-day adult		0
Ethanol 2.5% 3 hrs, larvae L3		0
Ethanol 5% 3 hrs, larvae L3		0
Ethanol 10% 3 hrs, larvae L3		0
Caffeine 1.5 mg/ml 4 hrs, larvae L3		0
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		0
Caffeine 25 mg/ml 48 hrs, 4-day adult		0
Paraquat 5 mM 48 hrs, 4-day adult		1
Paraquat 10 mM 48 hrs, 4-day adult		1
Rotenone 2 μg 12 hrs, larvae L3		0
Rotenone 8 μg 12 hrs, larvae L3		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010, Graveley et al., 2011)

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0063496

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG17525-RA

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable, with Scer\GAL4^pnr-MD237

GstE4^GD9405

Phenotype manifest in

Allele

Classical Alleles ( 0 )

For All Classical Alleles Show

Allele of GstE4	Class	Mutagen	Stocks	Known lesion

Alleles Carried on Transgenic Constructs ( 2 )

For All Alleles Carried on Transgenic Constructs Show

Allele of GstE4	Class	Mutagen	Stocks	Known lesion
GstE4^GD9405		in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
GstE4^KK106474		in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes

Aneuploid Aberrations

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD9405}

P{KK106474}

Insertions

Type of insertions

Name

Expression data

Gene Ontology: Function, Process & Cellular Component ( 3 unique terms )

Terms Based on Experimental Evidence ( 2 terms )

Molecular Function

CV term

Evidence

References

glutathione transferase activity

inferred from direct assay

(Saisawang et al., 2012)

Biological Process

CV term

Evidence

References

glutathione metabolic process

inferred from direct assay

(Saisawang et al., 2012)

Cellular Component ( 0 terms)

Terms Based on Predictions or Assertions ( 2 terms )

Molecular Function

CV term

Evidence

References

glutathione transferase activity

inferred from sequence or structural similarity with UniProtKB:P46430

(FlyBase, 1992-)

Biological Process ( 0 terms)

Cellular Component

CV term

Evidence

References

cytoplasm

inferred from sequence or structural similarity with rat Gsto1

(Curators, 2008--)

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

protein-protein

Interacting group

Assay

References

GstE4 - CG9890

anti tag coimmunoprecipitation, peptide massfingerprinting

(Mintseris et al., 2009.11.23)

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

GstE4 allele

Gene

References

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0063496

Orthologs

OrthoDB Orthologs (46) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG69GM30

Dipteran inclusive ortholog search

EOG6Q2DQN

Insect inclusive ortholog search

EOG6G79VV

Arthropod inclusive ortholog search

EOG6PNW0C

Metazoa inclusive ortholog search

EOG69D33R

Orthologs in Drosophila Species (EOG69GM30)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA14542

Drosophila persimilis

Dper\GL17771

Drosophila willistoni

Dwil\GK22983

Drosophila virilis

Dvir\GJ19891

Drosophila mojavensis

Dmoj\GI20121

Drosophila grimshawi

Dgri\GH21668

Orthologs in non-Drosophila Dipterans (EOG6Q2DQN)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ018628

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ018630

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ018629

Orthologs in non-Dipteran Insects (EOG6G79VV)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA002279

Bombyx mori

Silkmoth

Bmor\BGIBMGA010094

Bombyx mori

Silkmoth

Bmor\BGIBMGA010093

Bombyx mori

Silkmoth

Bmor\BGIBMGA009951

Tribolium castaneum

Red flour beetle

Tcas\TC004448

Tribolium castaneum

Red flour beetle

Tcas\TC004444

Tribolium castaneum

Red flour beetle

Tcas\TC003346

Tribolium castaneum

Red flour beetle

Tcas\TC004942

Tribolium castaneum

Red flour beetle

Tcas\TC004940

Tribolium castaneum

Red flour beetle

Tcas\TC003104

Tribolium castaneum

Red flour beetle

Tcas\TC003345

Tribolium castaneum

Red flour beetle

Tcas\TC004446

Tribolium castaneum

Red flour beetle

Tcas\TC003348

Tribolium castaneum

Red flour beetle

Tcas\TC004442

Tribolium castaneum

Red flour beetle

Tcas\TC003103

Tribolium castaneum

Red flour beetle

Tcas\TC004447

Tribolium castaneum

Red flour beetle

Tcas\TC004941

Tribolium castaneum

Red flour beetle

Tcas\TC004443

Tribolium castaneum

Red flour beetle

Tcas\TC004445

Tribolium castaneum

Red flour beetle

Tcas\TC004449

Orthologs in non-Insect Arthropods (EOG6PNW0C)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Ixodes scapularis

Deer tick

Isca\ISCW007012

Ixodes scapularis

Deer tick

Isca\ISCW015490

Ixodes scapularis

Deer tick

Isca\ISCW005805

Ixodes scapularis

Deer tick

Isca\ISCW008097

Ixodes scapularis

Deer tick

Isca\ISCW005804

Orthologs in non-Arthropod Metazoa (EOG69D33R)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_027539gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_006492gn

Mus musculus

House mouse

Mmus\Gstt2

Rattus norvegicus

Norway rat

Rnor\Gstt2

Homo sapiens

Human

Hsap\GSTT2

Homo sapiens

Human

Hsap\GSTT2B

Human Orthologs (2)

Gene

OMIM

HGNC

Hsap\GSTT2

Hsap\GSTT2B

AAA Orthologs (9) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA14542

Drosophila persimilis

Dper\GL17771

Drosophila willistoni

Dwil\GK22983

Drosophila virilis

Dvir\GJ19891

Stocks & Reagents

Stocks Listed in FlyBase ( 2 )

VDRC

v100986

P{KK106474}VIE-260B

v20472

w¹¹¹⁸; P{GD9405}v20472/TM3

Genomic Clones ( 1 )

BACR48A20

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 2 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Linkouts

DRSC - Results from RNAi screens.

•

FBgn0063496

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

37109

Antibody Information

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for database merge of

Additional comments

Other Comments

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_137482

RefSeq (Proteins)

•

NP_611326

DDBJ / EMBL / GenBank

•

AAF57698

Entrez Gene - A searchable database of RefSeq genes.

•

37109

UniProtKB/TrEMBL

•

A1ZB69

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Glutathione S-transferase, N-terminal (IPR004045)

Glutathione S-transferase, C-terminal (IPR004046)

Glutathione S-transferase, C-terminal-like (IPR010987)

Thioredoxin-like fold (IPR012336)

Glutathione S-transferase/chloride channel, C-terminal (IPR017933)

Linkouts

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0063496

DRSC - Results from RNAi screens.

•

FBgn0063496

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0063496

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG17525-RA

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0063496

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

37109

modMine - Data generated by the modENCODE project.

•

FBgn0063496

Synonyms & Secondary IDs ( 6 )

Reported As

Symbol Synonym

CG17525

(McGraw et al., 2004, Ranson et al., 2001, Keleman et al., 2009.8.5, Marco et al., 2004)

DmGSTE4

(Sawicki et al., 2003, Zimniak and Benes, 2002.7.15)

GstE4

GSTE4

(Low et al., 2007, Li et al., 2008)

gste4

(Saisawang et al., 2012)

Name Synonym

Glutathione S transferase E4

(Zimniak and Benes, 2002.7.15)

Secondary FlyBase IDs

FBgn0034338

References ( 19 )

Research paper

Saisawang et al., 2012, Biochem. J. 442(1): 181--190: A preliminary characterization of the cytosolic glutathione transferase proteome from Drosophila melanogaster. [FBrf0217315]
Li et al., 2008, Insect Mol. Biol. 17(4): 325--339: Transcriptomic profiles of Drosophila melanogaster third instar larval midgut and responses to oxidative stress. [FBrf0205872]
Low et al., 2007, Genetics 177(3): 1363--1375: Molecular evolution of glutathione S-transferases in the genus drosophila. [FBrf0202224]
Marco et al., 2004, Mol. Biol. Evol. 21(1): 176--187: Evolutionary and structural analyses of GDAP1, involved in Charcot-Marie-Tooth disease, characterize a novel class of glutathione transferase-related genes. [FBrf0212680]
McGraw et al., 2004, Curr. Biol. 14(16): 1509--1514: Genes regulated by mating, sperm, or seminal proteins in mated female Drosophila melanogaster. [FBrf0180059]
Sawicki et al., 2003, Biochem. J. 370(2): 661--669: Cloning, expression and biochemical characterization of one Epsilon-class (GST-3) and ten Delta-class (GST-1) glutathione S-transferases from Drosophila melanogaster, and identification of additional nine members of the Epsilon class. [FBrf0158728]
Ranson et al., 2001, Biochem. J. 359(2): 295--304: Identification of a novel class of insect glutathione S-transferases involved in resistance to DDT in the malaria vector Anopheles gambiae. [FBrf0151633]

Supplementary material

Graveley et al., 2011, Nature 471(7339):: Supplementary Table 33. Fuzzy Cluster Members. [FBrf0213980]
The modENCODE Consortium, 2010, Science 330(6012):: DataS14: Gene co-expression clusters. [FBrf0213979]
Mummery-Widmer et al., 2009, Nature 458(7241):: Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]

Personal communication to FlyBase

Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510]
Mintseris et al., 2009.11.23, DPiM: the Drosophila Protein Interaction Mapping project: DPiM: the Drosophila Protein Interaction Mapping project [FBrf0209452]
Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]
Zimniak and Benes, 2002.7.15, Insect GSTs.: Insect GSTs. [FBrf0150782]

FlyBase analysis

Curators, 2008--, Assigning Gene Ontology (GO) terms by sequence similarity in FlyBase.: Assigning Gene Ontology (GO) terms by sequence similarity in FlyBase. [FBrf0202953]
FlyBase, 1992-, FlyBase curation.: FlyBase curation. [FBrf0105495]

Curated genome annotation

FamiliarityBreedsContempt, 1999.11, [Automatic annotations performed at the Celera Jamboreee.]: [Automatic annotations performed at the Celera Jamboreee.] [FBrf0126705]
Gong, 1999.11, [Annotations performed at the Celera Jamboreee.]: [Annotations performed at the Celera Jamboreee.] [FBrf0126669]