Gene Dmel\RpL5

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General Information

Symbol

Dmel\RpL5

Species

D. melanogaster

Name

Ribosomal protein L5

Annotation symbol

CG17489

Feature type

protein_coding_gene

FlyBase ID

FBgn0064225

Gene Model Status

Current

Stock availability

2 publicly available

Also Known As

yip6, L5, E-2d

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

40F7-40F7

Sequence location

2L:22,426,649..22,429,419 [-]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

The gene Ribosomal protein L5 is referred to in FlyBase by the symbol Dmel\RpL5 (CG17489, FBgn0064225). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding; structural constituent of ribosome. Based on sequence similarity, it is predicted to be involved in the biological process: translation. 8 alleles are reported. The phenotypes of these alleles are annotated with: macrochaeta; adult heart; chaeta. It has 4 annotated transcripts and 4 annotated polypeptides. Protein features are: Ribosomal protein L18/L5; Ribosomal protein L5 eukaryotic/L18 archaeal; Ribosomal protein L5 eukaryotic/L18 archaeal, C-terminal. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of extremely high expression to a trough of very high expression. Peak expression observed at stages throughout embryogenesis, at stages throughout the larval period, at stages throughout the pupal period, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: High levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1631015_s_at completely aligns to an exonic region common to each of the 3 FlyBase-annotated transcript isoforms of RpL5. Gene sequence location is 2L:22427042..22429419.

User Contributed Data

Beta version, feedback welcome

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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FB2013_03

FB2013_02

All updates

Click here to see a list of all updates to this record from FB2010_08 and on.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

40F7-40F7

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

40A-40B

h35

(Drosophila Heterochromatin Genome Project, 2004)

Experimentally Determined Recombination Data

Location

(Coelho et al., 2005, Marygold et al., 2005)

Left of (cM)

Right of (cM)

Notes

Maps close to the centromere of the second chromosome.

(Marygold et al., 2005)

Gene Model & Products

Please see the GBrowse view of Dmel\RpL5 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Gene model reviewed during 5.45

Gene model reviewed during 5.51

Low-frequency RNA-Seq exon junction(s) not annotated.

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

RpL5-RA

FBtr0111129

NM_001042922

1047

299

RpL5-RB

FBtr0111132

NM_001042923

1024

299

RpL5-RG

FBtr0332633

NM_001042925

1945

299

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

RpL5-PA

FBpp0110421

34.0

299

10.30

NP_001036387

EAA46019

RpL5-PB

FBpp0110424

34.0

299

10.30

NP_001036388

EAA46016

RpL5-PG

FBpp0304879

34.0

299

10.30

NP_001036390

EAA46017

Additional Polypeptide Data & Comments

Reported size (kDa)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Ribosomal protein L5 eukaryotic/L18 archaeal, C-terminal (IPR025607)

Ribosomal protein L18/L5 (IPR005484)

Ribosomal protein L5 eukaryotic/L18 archaeal (IPR005485)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9W5R8

UniProtKB/TrEMBL

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

organism | ubiquitous

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

ribosome

inferred from direct assay

(Alonso and Santaren, 2006)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: High levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval/adult carcass.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		2452.6
Larval Midgut		4643.9
Larval Hindgut		4100
Larval Malpighian Tubules		5385.4
Larval Fat Body		7933
Larval Salivary Gland		7036.6
Larval Trachea		5919.4
Larval Carcass		4529.125
Adult Head		4831.9
Adult Eye		6498.5
Adult Brain		3452.8
Adult Thoracic-Abdominal Ganglion		4640.9
Adult Crop		6230.9
Adult Midgut		5525.1
Adult Hindgut		5415.4
Adult Malpighian Tubules		6671.1
Adult Fat Body		5288.5
Adult Salivary Gland		10492.1
Adult Heart		6576.775
Adult VirginFemale Spermatheca		5096.9
Adult InseminatedFemale Spermatheca		5209.2
Adult Ovary		3432.3
Adult Testis		1078.6
Adult Male Accessory Gland		9171.7
Adult Carcass		6044.4
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		(2452.6)
Larval Midgut		(4643.9)
Larval Hindgut		(4100)
Larval Malpighian Tubules		(5385.4)
Larval Fat Body		(7933)
Larval Salivary Gland		(7036.6)
Larval Trachea		(5919.4)
Larval Carcass		(4529.125)
Adult Head		(4831.9)
Adult Eye		(6498.5)
Adult Brain		(3452.8)
Adult Thoracic-Abdominal Ganglion		(4640.9)
Adult Crop		(6230.9)
Adult Midgut		(5525.1)
Adult Hindgut		(5415.4)
Adult Malpighian Tubules		(6671.1)
Adult Fat Body		(5288.5)
Adult Salivary Gland		(10492.1)
Adult Heart		(6576.775)
Adult VirginFemale Spermatheca		(5096.9)
Adult InseminatedFemale Spermatheca		(5209.2)
Adult Ovary		(3432.3)
Adult Testis		(1078.6)
Adult Male Accessory Gland		(9171.7)
Adult Carcass		(6044.4)
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		(2452.6)
Larval Midgut		(4643.9)
Larval Hindgut		(4100)
Larval Malpighian Tubules		(5385.4)
Larval Fat Body		(7933)
Larval Salivary Gland		(7036.6)
Larval Trachea		(5919.4)
Larval Carcass		(4529.125)
Adult Head		(4831.9)
Adult Eye		(6498.5)
Adult Brain		(3452.8)
Adult Thoracic-Abdominal Ganglion		(4640.9)
Adult Crop		(6230.9)
Adult Midgut		(5525.1)
Adult Hindgut		(5415.4)
Adult Malpighian Tubules		(6671.1)
Adult Fat Body		(5288.5)
Adult Salivary Gland		(10492.1)
Adult Heart		(6576.775)
Adult VirginFemale Spermatheca		(5096.9)
Adult InseminatedFemale Spermatheca		(5209.2)
Adult Ovary		(3432.3)
Adult Testis		(1078.6)
Adult Male Accessory Gland		(9171.7)
Adult Carcass		(6044.4)
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		2452.6
Larval Midgut		4643.9
Larval Hindgut		4100
Larval Malpighian Tubules		5385.4
Larval Fat Body		7933
Larval Salivary Gland		7036.6
Larval Trachea		5919.4
Larval Carcass		4529.125
Adult Head		4831.9
Adult Eye		6498.5
Adult Brain		3452.8
Adult Thoracic-Abdominal Ganglion		4640.9
Adult Crop		6230.9
Adult Midgut		5525.1
Adult Hindgut		5415.4
Adult Malpighian Tubules		6671.1
Adult Fat Body		5288.5
Adult Salivary Gland		10492.1
Adult Heart		6576.775
Adult VirginFemale Spermatheca		5096.9
Adult InseminatedFemale Spermatheca		5209.2
Adult Ovary		3432.3
Adult Testis		1078.6
Adult Male Accessory Gland		9171.7
Adult Carcass		6044.4
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		2452.6
Larval Midgut		4643.9
Larval Hindgut		4100
Larval Malpighian Tubules		5385.4
Larval Fat Body		7933
Larval Salivary Gland		7036.6
Larval Trachea		5919.4
Larval Carcass		4529.125
Adult Head		4831.9
Adult Eye		6498.5
Adult Brain		3452.8
Adult Thoracic-Abdominal Ganglion		4640.9
Adult Crop		6230.9
Adult Midgut		5525.1
Adult Hindgut		5415.4
Adult Malpighian Tubules		6671.1
Adult Fat Body		5288.5
Adult Salivary Gland		10492.1
Adult Heart		6576.775
Adult VirginFemale Spermatheca		5096.9
Adult InseminatedFemale Spermatheca		5209.2
Adult Ovary		3432.3
Adult Testis		1078.6
Adult Male Accessory Gland		9171.7
Adult Carcass		6044.4
Expression Level Scale		None Low Moderate High Very high

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		(2452.6)
Larval Midgut		(4643.9)
Larval Hindgut		(4100)
Larval Malpighian Tubules		(5385.4)
Larval Fat Body		(7933)
Larval Salivary Gland		(7036.6)
Larval Trachea		(5919.4)
Larval Carcass		(4529.125)
Adult Head		(4831.9)
Adult Eye		(6498.5)
Adult Brain		(3452.8)
Adult Thoracic-Abdominal Ganglion		(4640.9)
Adult Crop		(6230.9)
Adult Midgut		(5525.1)
Adult Hindgut		(5415.4)
Adult Malpighian Tubules		(6671.1)
Adult Fat Body		(5288.5)
Adult Salivary Gland		(10492.1)
Adult Heart		(6576.775)
Adult VirginFemale Spermatheca		(5096.9)
Adult InseminatedFemale Spermatheca		(5209.2)
Adult Ovary		(3432.3)
Adult Testis		(1078.6)
Adult Male Accessory Gland		(9171.7)
Adult Carcass		(6044.4)
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		(2452.6)
Larval Midgut		(4643.9)
Larval Hindgut		(4100)
Larval Malpighian Tubules		(5385.4)
Larval Fat Body		(7933)
Larval Salivary Gland		(7036.6)
Larval Trachea		(5919.4)
Larval Carcass		(4529.125)
Adult Head		(4831.9)
Adult Eye		(6498.5)
Adult Brain		(3452.8)
Adult Thoracic-Abdominal Ganglion		(4640.9)
Adult Crop		(6230.9)
Adult Midgut		(5525.1)
Adult Hindgut		(5415.4)
Adult Malpighian Tubules		(6671.1)
Adult Fat Body		(5288.5)
Adult Salivary Gland		(10492.1)
Adult Heart		(6576.775)
Adult VirginFemale Spermatheca		(5096.9)
Adult InseminatedFemale Spermatheca		(5209.2)
Adult Ovary		(3432.3)
Adult Testis		1078.6
Adult Male Accessory Gland		(9171.7)
Adult Carcass		(6044.4)
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		2452.6
Larval Midgut		4643.9
Larval Hindgut		4100
Larval Malpighian Tubules		5385.4
Larval Fat Body		7933
Larval Salivary Gland		7036.6
Larval Trachea		5919.4
Larval Carcass		4529.125
Adult Head		4831.9
Adult Eye		6498.5
Adult Brain		3452.8
Adult Thoracic-Abdominal Ganglion		4640.9
Adult Crop		6230.9
Adult Midgut		5525.1
Adult Hindgut		5415.4
Adult Malpighian Tubules		6671.1
Adult Fat Body		5288.5
Adult Salivary Gland		10492.1
Adult Heart		6576.775
Adult VirginFemale Spermatheca		5096.9
Adult InseminatedFemale Spermatheca		5209.2
Adult Ovary		3432.3
Adult Testis		1078.6
Adult Male Accessory Gland		9171.7
Adult Carcass		6044.4
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	4831.9
NA	Eye	6498.5
NA	Brain	3452.8
2452.6	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	4640.9
NA	Crop	6230.9
4643.9	Midgut	5525.1
4100	Hindgut	5415.4
5385.4	Malpighian Tubules	6671.1
7933	Fat Body	5288.5
7036.6	Salivary Gland	10492.1
NA	Heart	6576.775
5919.4	Trachea	NA
NA	VirginFemale Spermatheca	5096.9
NA	InseminatedFemale Spermatheca	5209.2
NA	Ovary	3432.3
NA	Testis	1078.6
NA	Male Accessory Gland	9171.7
4529.125	Carcass	6044.4

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		341
central nervous system, larvae L3		313
central nervous system, pupae P8		247
head, virgin 1-day female		308
head, virgin 4-day female		434
head, virgin 20-day female		523
head, mated 1-day female		280
head, mated 4-day female		542
head, mated 20-day female		397
head, mated 1-day male		296
head, mated 4-day male		389
head, mated 20-day male		561
salivary gland, larvae L3 wandering		442
salivary gland, white prepupae		840
digestive system, larvae L3 wandering		376
digestive system, 1-day adult		411
digestive system, 4-day adult		393
digestive system, 20-day adult		347
fat body, larvae L3 wandering		267
fat body, white prepupae		316
fat body, pupae P8		393
carcass, larvae L3 wandering		428
carcass, 1-day adult		264
carcass, 4-day adult		369
carcass, 20-day adult		323
ovary, virgin 4-day female		575
ovary, mated 4-day female		580
testis, mated 4-day male		137
accessory gland, mated 4-day male		341
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(341)
central nervous system, larvae L3		(313)
central nervous system, pupae P8		(247)
head, virgin 1-day female		(308)
head, virgin 4-day female		(434)
head, virgin 20-day female		(523)
head, mated 1-day female		(280)
head, mated 4-day female		(542)
head, mated 20-day female		(397)
head, mated 1-day male		(296)
head, mated 4-day male		(389)
head, mated 20-day male		(561)
salivary gland, larvae L3 wandering		(442)
salivary gland, white prepupae		(840)
digestive system, larvae L3 wandering		(376)
digestive system, 1-day adult		(411)
digestive system, 4-day adult		(393)
digestive system, 20-day adult		(347)
fat body, larvae L3 wandering		(267)
fat body, white prepupae		(316)
fat body, pupae P8		(393)
carcass, larvae L3 wandering		(428)
carcass, 1-day adult		(264)
carcass, 4-day adult		(369)
carcass, 20-day adult		(323)
ovary, virgin 4-day female		(575)
ovary, mated 4-day female		(580)
testis, mated 4-day male		(137)
accessory gland, mated 4-day male		(341)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(341)
central nervous system, larvae L3		(313)
central nervous system, pupae P8		(247)
head, virgin 1-day female		(308)
head, virgin 4-day female		(434)
head, virgin 20-day female		(523)
head, mated 1-day female		(280)
head, mated 4-day female		(542)
head, mated 20-day female		(397)
head, mated 1-day male		(296)
head, mated 4-day male		(389)
head, mated 20-day male		(561)
salivary gland, larvae L3 wandering		(442)
salivary gland, white prepupae		(840)
digestive system, larvae L3 wandering		(376)
digestive system, 1-day adult		(411)
digestive system, 4-day adult		(393)
digestive system, 20-day adult		(347)
fat body, larvae L3 wandering		(267)
fat body, white prepupae		(316)
fat body, pupae P8		(393)
carcass, larvae L3 wandering		(428)
carcass, 1-day adult		(264)
carcass, 4-day adult		(369)
carcass, 20-day adult		(323)
ovary, virgin 4-day female		(575)
ovary, mated 4-day female		(580)
testis, mated 4-day male		(137)
accessory gland, mated 4-day male		(341)
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		341
central nervous system, larvae L3		313
central nervous system, pupae P8		247
head, virgin 1-day female		308
head, virgin 4-day female		434
head, virgin 20-day female		523
head, mated 1-day female		280
head, mated 4-day female		542
head, mated 20-day female		397
head, mated 1-day male		296
head, mated 4-day male		389
head, mated 20-day male		561
salivary gland, larvae L3 wandering		442
salivary gland, white prepupae		840
digestive system, larvae L3 wandering		376
digestive system, 1-day adult		411
digestive system, 4-day adult		393
digestive system, 20-day adult		347
fat body, larvae L3 wandering		267
fat body, white prepupae		316
fat body, pupae P8		393
carcass, larvae L3 wandering		428
carcass, 1-day adult		264
carcass, 4-day adult		369
carcass, 20-day adult		323
ovary, virgin 4-day female		575
ovary, mated 4-day female		580
testis, mated 4-day male		137
accessory gland, mated 4-day male		341
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		341
central nervous system, larvae L3		313
central nervous system, pupae P8		247
head, virgin 1-day female		308
head, virgin 4-day female		434
head, virgin 20-day female		523
head, mated 1-day female		280
head, mated 4-day female		542
head, mated 20-day female		397
head, mated 1-day male		296
head, mated 4-day male		389
head, mated 20-day male		561
salivary gland, larvae L3 wandering		442
salivary gland, white prepupae		840
digestive system, larvae L3 wandering		376
digestive system, 1-day adult		411
digestive system, 4-day adult		393
digestive system, 20-day adult		347
fat body, larvae L3 wandering		267
fat body, white prepupae		316
fat body, pupae P8		393
carcass, larvae L3 wandering		428
carcass, 1-day adult		264
carcass, 4-day adult		369
carcass, 20-day adult		323
ovary, virgin 4-day female		575
ovary, mated 4-day female		580
testis, mated 4-day male		137
accessory gland, mated 4-day male		341
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(341)
central nervous system, larvae L3		(313)
central nervous system, pupae P8		(247)
head, virgin 1-day female		(308)
head, virgin 4-day female		(434)
head, virgin 20-day female		(523)
head, mated 1-day female		(280)
head, mated 4-day female		(542)
head, mated 20-day female		(397)
head, mated 1-day male		(296)
head, mated 4-day male		(389)
head, mated 20-day male		(561)
salivary gland, larvae L3 wandering		(442)
salivary gland, white prepupae		(840)
digestive system, larvae L3 wandering		(376)
digestive system, 1-day adult		(411)
digestive system, 4-day adult		(393)
digestive system, 20-day adult		(347)
fat body, larvae L3 wandering		(267)
fat body, white prepupae		(316)
fat body, pupae P8		(393)
carcass, larvae L3 wandering		(428)
carcass, 1-day adult		(264)
carcass, 4-day adult		(369)
carcass, 20-day adult		(323)
ovary, virgin 4-day female		(575)
ovary, mated 4-day female		(580)
testis, mated 4-day male		(137)
accessory gland, mated 4-day male		(341)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(341)
central nervous system, larvae L3		(313)
central nervous system, pupae P8		(247)
head, virgin 1-day female		(308)
head, virgin 4-day female		(434)
head, virgin 20-day female		(523)
head, mated 1-day female		(280)
head, mated 4-day female		(542)
head, mated 20-day female		(397)
head, mated 1-day male		(296)
head, mated 4-day male		(389)
head, mated 20-day male		(561)
salivary gland, larvae L3 wandering		(442)
salivary gland, white prepupae		(840)
digestive system, larvae L3 wandering		(376)
digestive system, 1-day adult		(411)
digestive system, 4-day adult		(393)
digestive system, 20-day adult		(347)
fat body, larvae L3 wandering		(267)
fat body, white prepupae		(316)
fat body, pupae P8		(393)
carcass, larvae L3 wandering		(428)
carcass, 1-day adult		(264)
carcass, 4-day adult		(369)
carcass, 20-day adult		(323)
ovary, virgin 4-day female		(575)
ovary, mated 4-day female		(580)
testis, mated 4-day male		137
accessory gland, mated 4-day male		(341)
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		341
central nervous system, larvae L3		313
central nervous system, pupae P8		247
head, virgin 1-day female		308
head, virgin 4-day female		434
head, virgin 20-day female		523
head, mated 1-day female		280
head, mated 4-day female		542
head, mated 20-day female		397
head, mated 1-day male		296
head, mated 4-day male		389
head, mated 20-day male		561
salivary gland, larvae L3 wandering		442
salivary gland, white prepupae		840
digestive system, larvae L3 wandering		376
digestive system, 1-day adult		411
digestive system, 4-day adult		393
digestive system, 20-day adult		347
fat body, larvae L3 wandering		267
fat body, white prepupae		316
fat body, pupae P8		393
carcass, larvae L3 wandering		428
carcass, 1-day adult		264
carcass, 4-day adult		369
carcass, 20-day adult		323
ovary, virgin 4-day female		575
ovary, mated 4-day female		580
testis, mated 4-day male		137
accessory gland, mated 4-day male		341
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of extremely high expression to a trough of very high expression. Peak expression observed at stages throughout embryogenesis, at stages throughout the larval period, at stages throughout the pupal period, in adult female stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		1043
embryo 02-04hr		1708
embryo 04-06hr		2021
embryo 06-08hr		1670
embryo 08-10hr		2144
embryo 10-12hr		2411
embryo 12-14hr		1968
embryo 14-16hr		1639
embryo 16-18hr		1622
embryo 18-20hr		1494
embryo 20-22hr		1750
embryo 22-24hr		2174
larva L1		1812
larva L2		1632
larva L3 12hr old		1250
larva L3 puffstage 1-2		1249
larva L3 puffstage 3-6		1131
larva L3 puffstage 7-9		1416
white prepupae new		1246
white prepupae 12hr		1222
white prepupae 24hr		1027
pupae 2d postWPP		912
pupae 3d postWPP		782
pupae 4d postWPP		685
adult male 01day		637
adult male 05day		555
adult male 30day		627
adult female 01day		1215
adult female 05day		1360
adult female 30day		1322
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(1043)
embryo 02-04hr		(1708)
embryo 04-06hr		(2021)
embryo 06-08hr		(1670)
embryo 08-10hr		(2144)
embryo 10-12hr		(2411)
embryo 12-14hr		(1968)
embryo 14-16hr		(1639)
embryo 16-18hr		(1622)
embryo 18-20hr		(1494)
embryo 20-22hr		(1750)
embryo 22-24hr		(2174)
larva L1		(1812)
larva L2		(1632)
larva L3 12hr old		(1250)
larva L3 puffstage 1-2		(1249)
larva L3 puffstage 3-6		(1131)
larva L3 puffstage 7-9		(1416)
white prepupae new		(1246)
white prepupae 12hr		(1222)
white prepupae 24hr		(1027)
pupae 2d postWPP		(912)
pupae 3d postWPP		(782)
pupae 4d postWPP		(685)
adult male 01day		(637)
adult male 05day		(555)
adult male 30day		(627)
adult female 01day		(1215)
adult female 05day		(1360)
adult female 30day		(1322)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		(1043)
embryo 02-04hr		(1708)
embryo 04-06hr		(2021)
embryo 06-08hr		(1670)
embryo 08-10hr		(2144)
embryo 10-12hr		(2411)
embryo 12-14hr		(1968)
embryo 14-16hr		(1639)
embryo 16-18hr		(1622)
embryo 18-20hr		(1494)
embryo 20-22hr		(1750)
embryo 22-24hr		(2174)
larva L1		(1812)
larva L2		(1632)
larva L3 12hr old		(1250)
larva L3 puffstage 1-2		(1249)
larva L3 puffstage 3-6		(1131)
larva L3 puffstage 7-9		(1416)
white prepupae new		(1246)
white prepupae 12hr		(1222)
white prepupae 24hr		(1027)
pupae 2d postWPP		(912)
pupae 3d postWPP		(782)
pupae 4d postWPP		(685)
adult male 01day		(637)
adult male 05day		(555)
adult male 30day		(627)
adult female 01day		(1215)
adult female 05day		(1360)
adult female 30day		(1322)
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		1043
embryo 02-04hr		1708
embryo 04-06hr		2021
embryo 06-08hr		1670
embryo 08-10hr		2144
embryo 10-12hr		2411
embryo 12-14hr		1968
embryo 14-16hr		1639
embryo 16-18hr		1622
embryo 18-20hr		1494
embryo 20-22hr		1750
embryo 22-24hr		2174
larva L1		1812
larva L2		1632
larva L3 12hr old		1250
larva L3 puffstage 1-2		1249
larva L3 puffstage 3-6		1131
larva L3 puffstage 7-9		1416
white prepupae new		1246
white prepupae 12hr		1222
white prepupae 24hr		1027
pupae 2d postWPP		912
pupae 3d postWPP		782
pupae 4d postWPP		685
adult male 01day		637
adult male 05day		555
adult male 30day		627
adult female 01day		1215
adult female 05day		1360
adult female 30day		1322
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		1043
embryo 02-04hr		1708
embryo 04-06hr		2021
embryo 06-08hr		1670
embryo 08-10hr		2144
embryo 10-12hr		2411
embryo 12-14hr		1968
embryo 14-16hr		1639
embryo 16-18hr		1622
embryo 18-20hr		1494
embryo 20-22hr		1750
embryo 22-24hr		2174
larva L1		1812
larva L2		1632
larva L3 12hr old		1250
larva L3 puffstage 1-2		1249
larva L3 puffstage 3-6		1131
larva L3 puffstage 7-9		1416
white prepupae new		1246
white prepupae 12hr		1222
white prepupae 24hr		1027
pupae 2d postWPP		912
pupae 3d postWPP		782
pupae 4d postWPP		685
adult male 01day		637
adult male 05day		555
adult male 30day		627
adult female 01day		1215
adult female 05day		1360
adult female 30day		1322
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(1043)
embryo 02-04hr		(1708)
embryo 04-06hr		(2021)
embryo 06-08hr		(1670)
embryo 08-10hr		(2144)
embryo 10-12hr		(2411)
embryo 12-14hr		(1968)
embryo 14-16hr		(1639)
embryo 16-18hr		(1622)
embryo 18-20hr		(1494)
embryo 20-22hr		(1750)
embryo 22-24hr		(2174)
larva L1		(1812)
larva L2		(1632)
larva L3 12hr old		(1250)
larva L3 puffstage 1-2		(1249)
larva L3 puffstage 3-6		(1131)
larva L3 puffstage 7-9		(1416)
white prepupae new		(1246)
white prepupae 12hr		(1222)
white prepupae 24hr		(1027)
pupae 2d postWPP		(912)
pupae 3d postWPP		(782)
pupae 4d postWPP		(685)
adult male 01day		(637)
adult male 05day		(555)
adult male 30day		(627)
adult female 01day		(1215)
adult female 05day		(1360)
adult female 30day		(1322)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		(1043)
embryo 02-04hr		(1708)
embryo 04-06hr		(2021)
embryo 06-08hr		(1670)
embryo 08-10hr		(2144)
embryo 10-12hr		(2411)
embryo 12-14hr		(1968)
embryo 14-16hr		(1639)
embryo 16-18hr		(1622)
embryo 18-20hr		(1494)
embryo 20-22hr		(1750)
embryo 22-24hr		(2174)
larva L1		(1812)
larva L2		(1632)
larva L3 12hr old		(1250)
larva L3 puffstage 1-2		(1249)
larva L3 puffstage 3-6		(1131)
larva L3 puffstage 7-9		(1416)
white prepupae new		(1246)
white prepupae 12hr		(1222)
white prepupae 24hr		(1027)
pupae 2d postWPP		(912)
pupae 3d postWPP		(782)
pupae 4d postWPP		(685)
adult male 01day		(637)
adult male 05day		(555)
adult male 30day		(627)
adult female 01day		(1215)
adult female 05day		(1360)
adult female 30day		(1322)
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		1043
embryo 02-04hr		1708
embryo 04-06hr		2021
embryo 06-08hr		1670
embryo 08-10hr		2144
embryo 10-12hr		2411
embryo 12-14hr		1968
embryo 14-16hr		1639
embryo 16-18hr		1622
embryo 18-20hr		1494
embryo 20-22hr		1750
embryo 22-24hr		2174
larva L1		1812
larva L2		1632
larva L3 12hr old		1250
larva L3 puffstage 1-2		1249
larva L3 puffstage 3-6		1131
larva L3 puffstage 7-9		1416
white prepupae new		1246
white prepupae 12hr		1222
white prepupae 24hr		1027
pupae 2d postWPP		912
pupae 3d postWPP		782
pupae 4d postWPP		685
adult male 01day		637
adult male 05day		555
adult male 30day		627
adult female 01day		1215
adult female 05day		1360
adult female 30day		1322
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		4628
Schneider line 2 Sg4		1522
embryonic 1182-4H		1020
embryonic GM2		1527
embryonic Kc167		2271
embryonic S1		1349
embryonic S3		899
leg disc CME L1		1273
wing disc CME-W2		1156
wing disc ML-DmD8		1626
wing disc ML-DmD9		1229
wing disc ML-DmD16-c3		913
wing disc ML-DmD21		3216
wing disc ML-DmD32		584
haltere disc ML-DmD17-c3		773
eye-antennal disc ML-DmD11		1252
antennal disc ML-DmD20-c5		1379
mixed discs ML-DmD4-c1		1073
CNS ML-DmBG1-c1		903
CNS ML-DmBG2-c2		2382
tumorous blood cells mbn2		2808
ovary fGS/OSS		725
ovary OSC		557
ovary OSS		383
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(4628)
Schneider line 2 Sg4		(1522)
embryonic 1182-4H		(1020)
embryonic GM2		(1527)
embryonic Kc167		(2271)
embryonic S1		(1349)
embryonic S3		(899)
leg disc CME L1		(1273)
wing disc CME-W2		(1156)
wing disc ML-DmD8		(1626)
wing disc ML-DmD9		(1229)
wing disc ML-DmD16-c3		(913)
wing disc ML-DmD21		(3216)
wing disc ML-DmD32		(584)
haltere disc ML-DmD17-c3		(773)
eye-antennal disc ML-DmD11		(1252)
antennal disc ML-DmD20-c5		(1379)
mixed discs ML-DmD4-c1		(1073)
CNS ML-DmBG1-c1		(903)
CNS ML-DmBG2-c2		(2382)
tumorous blood cells mbn2		(2808)
ovary fGS/OSS		(725)
ovary OSC		(557)
ovary OSS		(383)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		(4628)
Schneider line 2 Sg4		(1522)
embryonic 1182-4H		(1020)
embryonic GM2		(1527)
embryonic Kc167		(2271)
embryonic S1		(1349)
embryonic S3		(899)
leg disc CME L1		(1273)
wing disc CME-W2		(1156)
wing disc ML-DmD8		(1626)
wing disc ML-DmD9		(1229)
wing disc ML-DmD16-c3		(913)
wing disc ML-DmD21		(3216)
wing disc ML-DmD32		(584)
haltere disc ML-DmD17-c3		(773)
eye-antennal disc ML-DmD11		(1252)
antennal disc ML-DmD20-c5		(1379)
mixed discs ML-DmD4-c1		(1073)
CNS ML-DmBG1-c1		(903)
CNS ML-DmBG2-c2		(2382)
tumorous blood cells mbn2		(2808)
ovary fGS/OSS		(725)
ovary OSC		(557)
ovary OSS		(383)
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		4628
Schneider line 2 Sg4		1522
embryonic 1182-4H		1020
embryonic GM2		1527
embryonic Kc167		2271
embryonic S1		1349
embryonic S3		899
leg disc CME L1		1273
wing disc CME-W2		1156
wing disc ML-DmD8		1626
wing disc ML-DmD9		1229
wing disc ML-DmD16-c3		913
wing disc ML-DmD21		3216
wing disc ML-DmD32		584
haltere disc ML-DmD17-c3		773
eye-antennal disc ML-DmD11		1252
antennal disc ML-DmD20-c5		1379
mixed discs ML-DmD4-c1		1073
CNS ML-DmBG1-c1		903
CNS ML-DmBG2-c2		2382
tumorous blood cells mbn2		2808
ovary fGS/OSS		725
ovary OSC		557
ovary OSS		383
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		4628
Schneider line 2 Sg4		1522
embryonic 1182-4H		1020
embryonic GM2		1527
embryonic Kc167		2271
embryonic S1		1349
embryonic S3		899
leg disc CME L1		1273
wing disc CME-W2		1156
wing disc ML-DmD8		1626
wing disc ML-DmD9		1229
wing disc ML-DmD16-c3		913
wing disc ML-DmD21		3216
wing disc ML-DmD32		584
haltere disc ML-DmD17-c3		773
eye-antennal disc ML-DmD11		1252
antennal disc ML-DmD20-c5		1379
mixed discs ML-DmD4-c1		1073
CNS ML-DmBG1-c1		903
CNS ML-DmBG2-c2		2382
tumorous blood cells mbn2		2808
ovary fGS/OSS		725
ovary OSC		557
ovary OSS		383
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(4628)
Schneider line 2 Sg4		(1522)
embryonic 1182-4H		(1020)
embryonic GM2		(1527)
embryonic Kc167		(2271)
embryonic S1		(1349)
embryonic S3		(899)
leg disc CME L1		(1273)
wing disc CME-W2		(1156)
wing disc ML-DmD8		(1626)
wing disc ML-DmD9		(1229)
wing disc ML-DmD16-c3		(913)
wing disc ML-DmD21		(3216)
wing disc ML-DmD32		(584)
haltere disc ML-DmD17-c3		(773)
eye-antennal disc ML-DmD11		(1252)
antennal disc ML-DmD20-c5		(1379)
mixed discs ML-DmD4-c1		(1073)
CNS ML-DmBG1-c1		(903)
CNS ML-DmBG2-c2		(2382)
tumorous blood cells mbn2		(2808)
ovary fGS/OSS		(725)
ovary OSC		(557)
ovary OSS		(383)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		(4628)
Schneider line 2 Sg4		(1522)
embryonic 1182-4H		(1020)
embryonic GM2		(1527)
embryonic Kc167		(2271)
embryonic S1		(1349)
embryonic S3		(899)
leg disc CME L1		(1273)
wing disc CME-W2		(1156)
wing disc ML-DmD8		(1626)
wing disc ML-DmD9		(1229)
wing disc ML-DmD16-c3		(913)
wing disc ML-DmD21		(3216)
wing disc ML-DmD32		(584)
haltere disc ML-DmD17-c3		(773)
eye-antennal disc ML-DmD11		(1252)
antennal disc ML-DmD20-c5		(1379)
mixed discs ML-DmD4-c1		(1073)
CNS ML-DmBG1-c1		(903)
CNS ML-DmBG2-c2		(2382)
tumorous blood cells mbn2		(2808)
ovary fGS/OSS		(725)
ovary OSC		(557)
ovary OSS		(383)
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		4628
Schneider line 2 Sg4		1522
embryonic 1182-4H		1020
embryonic GM2		1527
embryonic Kc167		2271
embryonic S1		1349
embryonic S3		899
leg disc CME L1		1273
wing disc CME-W2		1156
wing disc ML-DmD8		1626
wing disc ML-DmD9		1229
wing disc ML-DmD16-c3		913
wing disc ML-DmD21		3216
wing disc ML-DmD32		584
haltere disc ML-DmD17-c3		773
eye-antennal disc ML-DmD11		1252
antennal disc ML-DmD20-c5		1379
mixed discs ML-DmD4-c1		1073
CNS ML-DmBG1-c1		903
CNS ML-DmBG2-c2		2382
tumorous blood cells mbn2		2808
ovary fGS/OSS		725
ovary OSC		557
ovary OSS		383
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		313
cold shock, 4-day adult		274
heat shock, 4-day adult		802
Cadmium 50 mM 6 hrs, larvae L3		718
Cadmium 50 mM 12 hrs, larvae L3		879
Cadmium 50 mM 48 hrs, 4-day adult		597
Cadmium 100 mM 48 hrs, 4-day adult		581
Copper 0.5 mM 12 hrs, larvae L3		404
Copper 15 mM 48 hrs, 4-day adult		481
Zinc 5 mM 12 hrs, larvae L3		276
Zinc 4.5 mM 48 hrs, 4-day adult		448
Ethanol 2.5% 3 hrs, larvae L3		799
Ethanol 5% 3 hrs, larvae L3		874
Ethanol 10% 3 hrs, larvae L3		706
Caffeine 1.5 mg/ml 4 hrs, larvae L3		757
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		562
Caffeine 25 mg/ml 48 hrs, 4-day adult		306
Paraquat 5 mM 48 hrs, 4-day adult		676
Paraquat 10 mM 48 hrs, 4-day adult		790
Rotenone 2 μg 12 hrs, larvae L3		804
Rotenone 8 μg 12 hrs, larvae L3		431
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		(313)
cold shock, 4-day adult		(274)
heat shock, 4-day adult		(802)
Cadmium 50 mM 6 hrs, larvae L3		(718)
Cadmium 50 mM 12 hrs, larvae L3		(879)
Cadmium 50 mM 48 hrs, 4-day adult		(597)
Cadmium 100 mM 48 hrs, 4-day adult		(581)
Copper 0.5 mM 12 hrs, larvae L3		(404)
Copper 15 mM 48 hrs, 4-day adult		(481)
Zinc 5 mM 12 hrs, larvae L3		(276)
Zinc 4.5 mM 48 hrs, 4-day adult		(448)
Ethanol 2.5% 3 hrs, larvae L3		(799)
Ethanol 5% 3 hrs, larvae L3		(874)
Ethanol 10% 3 hrs, larvae L3		(706)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(757)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(562)
Caffeine 25 mg/ml 48 hrs, 4-day adult		(306)
Paraquat 5 mM 48 hrs, 4-day adult		(676)
Paraquat 10 mM 48 hrs, 4-day adult		(790)
Rotenone 2 μg 12 hrs, larvae L3		(804)
Rotenone 8 μg 12 hrs, larvae L3		(431)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		(313)
cold shock, 4-day adult		(274)
heat shock, 4-day adult		(802)
Cadmium 50 mM 6 hrs, larvae L3		(718)
Cadmium 50 mM 12 hrs, larvae L3		(879)
Cadmium 50 mM 48 hrs, 4-day adult		(597)
Cadmium 100 mM 48 hrs, 4-day adult		(581)
Copper 0.5 mM 12 hrs, larvae L3		(404)
Copper 15 mM 48 hrs, 4-day adult		(481)
Zinc 5 mM 12 hrs, larvae L3		(276)
Zinc 4.5 mM 48 hrs, 4-day adult		(448)
Ethanol 2.5% 3 hrs, larvae L3		(799)
Ethanol 5% 3 hrs, larvae L3		(874)
Ethanol 10% 3 hrs, larvae L3		(706)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(757)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(562)
Caffeine 25 mg/ml 48 hrs, 4-day adult		(306)
Paraquat 5 mM 48 hrs, 4-day adult		(676)
Paraquat 10 mM 48 hrs, 4-day adult		(790)
Rotenone 2 μg 12 hrs, larvae L3		(804)
Rotenone 8 μg 12 hrs, larvae L3		(431)
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		313
cold shock, 4-day adult		274
heat shock, 4-day adult		802
Cadmium 50 mM 6 hrs, larvae L3		718
Cadmium 50 mM 12 hrs, larvae L3		879
Cadmium 50 mM 48 hrs, 4-day adult		597
Cadmium 100 mM 48 hrs, 4-day adult		581
Copper 0.5 mM 12 hrs, larvae L3		404
Copper 15 mM 48 hrs, 4-day adult		481
Zinc 5 mM 12 hrs, larvae L3		276
Zinc 4.5 mM 48 hrs, 4-day adult		448
Ethanol 2.5% 3 hrs, larvae L3		799
Ethanol 5% 3 hrs, larvae L3		874
Ethanol 10% 3 hrs, larvae L3		706
Caffeine 1.5 mg/ml 4 hrs, larvae L3		757
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		562
Caffeine 25 mg/ml 48 hrs, 4-day adult		306
Paraquat 5 mM 48 hrs, 4-day adult		676
Paraquat 10 mM 48 hrs, 4-day adult		790
Rotenone 2 μg 12 hrs, larvae L3		804
Rotenone 8 μg 12 hrs, larvae L3		431
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		313
cold shock, 4-day adult		274
heat shock, 4-day adult		802
Cadmium 50 mM 6 hrs, larvae L3		718
Cadmium 50 mM 12 hrs, larvae L3		879
Cadmium 50 mM 48 hrs, 4-day adult		597
Cadmium 100 mM 48 hrs, 4-day adult		581
Copper 0.5 mM 12 hrs, larvae L3		404
Copper 15 mM 48 hrs, 4-day adult		481
Zinc 5 mM 12 hrs, larvae L3		276
Zinc 4.5 mM 48 hrs, 4-day adult		448
Ethanol 2.5% 3 hrs, larvae L3		799
Ethanol 5% 3 hrs, larvae L3		874
Ethanol 10% 3 hrs, larvae L3		706
Caffeine 1.5 mg/ml 4 hrs, larvae L3		757
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		562
Caffeine 25 mg/ml 48 hrs, 4-day adult		306
Paraquat 5 mM 48 hrs, 4-day adult		676
Paraquat 10 mM 48 hrs, 4-day adult		790
Rotenone 2 μg 12 hrs, larvae L3		804
Rotenone 8 μg 12 hrs, larvae L3		431
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		(313)
cold shock, 4-day adult		(274)
heat shock, 4-day adult		(802)
Cadmium 50 mM 6 hrs, larvae L3		(718)
Cadmium 50 mM 12 hrs, larvae L3		(879)
Cadmium 50 mM 48 hrs, 4-day adult		(597)
Cadmium 100 mM 48 hrs, 4-day adult		(581)
Copper 0.5 mM 12 hrs, larvae L3		(404)
Copper 15 mM 48 hrs, 4-day adult		(481)
Zinc 5 mM 12 hrs, larvae L3		(276)
Zinc 4.5 mM 48 hrs, 4-day adult		(448)
Ethanol 2.5% 3 hrs, larvae L3		(799)
Ethanol 5% 3 hrs, larvae L3		(874)
Ethanol 10% 3 hrs, larvae L3		(706)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(757)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(562)
Caffeine 25 mg/ml 48 hrs, 4-day adult		(306)
Paraquat 5 mM 48 hrs, 4-day adult		(676)
Paraquat 10 mM 48 hrs, 4-day adult		(790)
Rotenone 2 μg 12 hrs, larvae L3		(804)
Rotenone 8 μg 12 hrs, larvae L3		(431)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		(313)
cold shock, 4-day adult		(274)
heat shock, 4-day adult		(802)
Cadmium 50 mM 6 hrs, larvae L3		(718)
Cadmium 50 mM 12 hrs, larvae L3		(879)
Cadmium 50 mM 48 hrs, 4-day adult		(597)
Cadmium 100 mM 48 hrs, 4-day adult		(581)
Copper 0.5 mM 12 hrs, larvae L3		(404)
Copper 15 mM 48 hrs, 4-day adult		(481)
Zinc 5 mM 12 hrs, larvae L3		(276)
Zinc 4.5 mM 48 hrs, 4-day adult		(448)
Ethanol 2.5% 3 hrs, larvae L3		(799)
Ethanol 5% 3 hrs, larvae L3		(874)
Ethanol 10% 3 hrs, larvae L3		(706)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(757)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(562)
Caffeine 25 mg/ml 48 hrs, 4-day adult		(306)
Paraquat 5 mM 48 hrs, 4-day adult		(676)
Paraquat 10 mM 48 hrs, 4-day adult		(790)
Rotenone 2 μg 12 hrs, larvae L3		(804)
Rotenone 8 μg 12 hrs, larvae L3		(431)
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		313
cold shock, 4-day adult		274
heat shock, 4-day adult		802
Cadmium 50 mM 6 hrs, larvae L3		718
Cadmium 50 mM 12 hrs, larvae L3		879
Cadmium 50 mM 48 hrs, 4-day adult		597
Cadmium 100 mM 48 hrs, 4-day adult		581
Copper 0.5 mM 12 hrs, larvae L3		404
Copper 15 mM 48 hrs, 4-day adult		481
Zinc 5 mM 12 hrs, larvae L3		276
Zinc 4.5 mM 48 hrs, 4-day adult		448
Ethanol 2.5% 3 hrs, larvae L3		799
Ethanol 5% 3 hrs, larvae L3		874
Ethanol 10% 3 hrs, larvae L3		706
Caffeine 1.5 mg/ml 4 hrs, larvae L3		757
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		562
Caffeine 25 mg/ml 48 hrs, 4-day adult		306
Paraquat 5 mM 48 hrs, 4-day adult		676
Paraquat 10 mM 48 hrs, 4-day adult		790
Rotenone 2 μg 12 hrs, larvae L3		804
Rotenone 8 μg 12 hrs, larvae L3		431
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010, Graveley et al., 2011)

External Data & Images

Linkouts

BDGP expression data

•

RE33114

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0064225

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG17489-RE

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal | larval stage, with Scer\GAL4^amn-c651

RpL5^{dsRNA.WIZ.Scer\UAS}

lethal | larval stage (with RpL5^2d1)

RpL5^2d2

lethal | larval stage (with RpL5^2d2)

RpL5^2d1

lethal | larval stage | recessive

RpL5^2d1

Other Phenotypes

Allele

decreased cell number | dominant

developmental rate defective | dominant

increased cell size | dominant

Minute

RpL5^2d2

Minute | dominant

size defective | adult stage

RpL5^2d2

small body | larval stage (with RpL5^2d1)

RpL5^2d2

small body | larval stage (with RpL5^2d2)

RpL5^2d1

Phenotype manifest in

Allele

adult heart

RpL5^2d2

chaeta

macrochaeta

RpL5^2d2

Classical Alleles ( 3 )

For All Classical Alleles Show

Allele of RpL5	Mutagen	Stocks	Known lesion
RpL5^2d1	ethyl methanesulfonate	1	Yes
RpL5^2d2	ethyl methanesulfonate	1	Yes
RpL5^Wee-P28		0	Yes

Alleles Carried on Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Allele of RpL5	Mutagen	Known lesion
RpL5^dsRNA.cDa	in vitro construct - RNAi	Yes
RpL5^{dsRNA.WIZ.Scer\UAS}	in vitro construct - regulatory fusion in vitro construct - RNAi	Yes
RpL5^KK115256	in vitro construct - RNAi in vitro construct - regulatory fusion	Yes
RpL5^{MtnA.T:Hsap\MYC}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	Yes
RpL5^NIG.17489R	in vitro construct - RNAi in vitro construct - regulatory fusion	Yes

Aneuploid Aberrations

Disrupted in

Df(2L)lt31

(Marygold et al., 2005)

Df(2L)lt64

(Marygold et al., 2005)

Not disrupted in

Df(2L)lt5

(Marygold et al., 2005)

Df(2L)lt68

(Marygold et al., 2005)

Df(2L)TW161

(Marygold et al., 2005)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{KK115256}

P{NIG.17489R}

P{UAS-RpL5.RNAi}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

P{Wee-P.ph0}RpL5^Wee-P28

Gene Ontology: Function, Process & Cellular Component ( 6 unique terms )

Terms Based on Experimental Evidence ( 3 terms )

Molecular Function

CV term

Evidence

References

protein binding

inferred from physical interaction with Fmr1

(Ishizuka et al., 2002)

structural constituent of ribosome

inferred from direct assay

(Alonso and Santaren, 2006)

Biological Process ( 0 terms)

Cellular Component

CV term

Evidence

References

ribosome

inferred from direct assay

(Alonso and Santaren, 2006)

Terms Based on Predictions or Assertions ( 4 terms )

Molecular Function

CV term

Evidence

References

5S rRNA binding

inferred from electronic annotation with InterPro:IPR005485

(FlyBase Curators et al., 2004-)

non-traceable author statement

(Ishizuka et al., 2002)

structural constituent of ribosome

inferred from sequence or structural similarity

(Roxstrom-Lindquist, 1999.10.15)

non-traceable author statement

(Ishizuka et al., 2002)

Biological Process

CV term

Evidence

References

translation

inferred from electronic annotation with InterPro:IPR005484, InterPro:IPR005485

(FlyBase Curators et al., 2004-)

inferred from sequence or structural similarity

(Roxstrom-Lindquist, 1999.10.15)

Cellular Component

CV term

Evidence

References

cytosolic large ribosomal subunit

inferred from sequence or structural similarity with mouse Rpl5

(FlyBase, 1992-)

non-traceable author statement

(Ishizuka et al., 2002)

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

protein-protein

Interacting group

Assay

References

RpL5 - CG14556

anti tag coimmunoprecipitation, peptide massfingerprinting

(Mintseris et al., 2009.11.23)

RpL5 - CG1492

anti tag coimmunoprecipitation, peptide massfingerprinting

(Mintseris et al., 2009.11.23)

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

RpL5 allele

Gene

References

unspecified

Pi3K92E

(Marygold et al., 2005, Coelho et al., 2005)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions

•

78249

DPiM - DPiM, Drosophila Protein interaction Map

•

FBgn0064225

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0064225

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

3355124

Orthologs

OrthoDB Orthologs (39) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG6VDQJK

Dipteran inclusive ortholog search

EOG6RBQTC

Insect inclusive ortholog search

EOG6H9WGC

Arthropod inclusive ortholog search

EOG6V9S6F

Metazoa inclusive ortholog search

EOG6JTC0D

Orthologs in Drosophila Species (EOG6VDQJK)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA28757

Drosophila persimilis

Dper\GL21177

Drosophila willistoni

Dwil\GK24660

Orthologs in non-Drosophila Dipterans (EOG6RBQTC)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Aedes aegypti

Yellow fever mosquito

Aaeg\RpL5

Anopheles gambiae

Malaria mosquito

Agam\AGAP009031

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ801753

Orthologs in non-Dipteran Insects (EOG6H9WGC)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis mellifera

Western honey bee

Amel\GB52256

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG005272

Atta cephalotes

Leafcutter ant

Acep\ACEP27130

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO16078

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL18591

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL22614

Linepithema humile

Argentine ant

Lhum\LH23270

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB18007

Solenopsis invicta

Red fire ant

Sinv\SINV23492

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI003593

Bombyx mori

Silkmoth

Bmor\BGIBMGA007879

Pediculus humanus

Human body louse

Phum\PHUM210740

Tribolium castaneum

Red flour beetle

Tcas\TC011241

Orthologs in non-Insect Arthropods (EOG6V9S6F)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Daphnia pulex

Water flea

Dpux\JGI_V11_299917

Ixodes scapularis

Deer tick

Isca\ISCW017610

Orthologs in non-Arthropod Metazoa (EOG6JTC0D)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Caenorhabditis elegans

Nematode

Cele\rpl-5

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_013432gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_001314gn

Danio rerio

Zebrafish

Drer\rpl5b

Danio rerio

Zebrafish

Drer\rpl5a

Xenopus tropicalis

Western clawed frog

Xtro\rpl5

Gallus gallus

Domestic chicken

Ggal\RPL5

Mus musculus

House mouse

Mmus\Rpl5

Rattus norvegicus

Norway rat

Rnor\RGD1564051

Rattus norvegicus

Norway rat

Rnor\Rpl5

Rattus norvegicus

Norway rat

Rnor\ENSRNOG00000042839

Rattus norvegicus

Norway rat

Rnor\ENSRNOG00000042479

Homo sapiens

Human

Hsap\RPL5

Human Orthologs (1)

Gene

OMIM

HGNC

Hsap\RPL5

AAA Orthologs (0) based on analysis using Dmel annotation version 4.3

No orthologs identified

Stocks & Reagents

Stocks Listed in FlyBase ( 2 )

Bloomington

25907

w^*; RpL5^2d2/CyO, P{GAL4-Kr.C}DC3, P{UAS-GFP.S65T}DC7

25910

w^*; RpL5^2d1/CyO, P{GAL4-Kr.C}DC3, P{UAS-GFP.S65T}DC7

Genomic Clones ( 1 )

BACR01K02

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 1288 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones