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Gene phiC31\int

General Information
Symbol	phiC31\int	Species	f. Streptomyces bacteriophage fC31
Name	bacteriophage phi-C31 integrase	Annotation symbol
Feature type	engineered_foreign_gene	FlyBase ID	FBgn0069090
Gene Model Status	Not Applicable	Stock availability	66 publicly available
Genomic Location
Chromosome (arm)		Recombination map
Cytogenetic map		Sequence location
Summary Information
Automatically generated summary See sections below for more information	The gene bacteriophage phi-C31 integrase is referred to in FlyBase by the symbol phiC31\int (FBgn0069090). It is a engineered_foreign_gene from fC31-like viruses Streptomyces bacteriophage fC31. Its molecular function is unknown. The biological processes in which it is involved are not known. 7 alleles are reported. No phenotypic data is available. It has no annotated transcripts. Gene has not been localized to the genome sequence.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa)
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL
Mapped Features
Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
modENCODE Anatomy RNA-Seq
modENCODE Development RNA-Seq
modENCODE Cell Lines RNA-Seq
modENCODE Treatments RNA-Seq
Expression Clusters
External Data & Images
Linkouts
Alleles & Phenotypes
Summary of Allele Phenotypes
Phenotype manifest in Allele
Classical Alleles ( 0 )
For All Classical Alleles Show
Allele of phiC31\int Class Mutagen Stocks Known lesion
Alleles Carried on Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Allele of phiC31\int Class Mutagen Stocks Known lesion phiC31\intDm.vas in vitro construct - regulatory fusion in vitro construct - site directed mutagenesis 46 Yes phiC31\intnos.T:SV40\nls2 in vitro construct - regulatory fusion in vitro construct - coding region fusion 12 Yes phiC31\intvas.PB in vitro construct - regulatory fusion in vitro construct - coding region fusion 9 Yes phiC31\intcGa in vitro construct 0 Yes phiC31\intMtnA.PG in vitro construct - regulatory fusion 0 Yes phiC31\intnos.PB in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes phiC31\intvas.T:SV40\nls2 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 0 unique terms )
Terms Based on Experimental Evidence ( 0 terms )
Terms Based on Predictions or Assertions ( 0 terms )
Sequence Ontology: Class of Gene
	engineered_foreign_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions phiC31\int allele Gene References
External Data
Linkouts
Orthologs
OrthoDB Orthologs (0) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search No orthologs identified Dipteran inclusive ortholog search No orthologs identified Insect inclusive ortholog search No orthologs identified Arthropod inclusive ortholog search No orthologs identified Metazoa inclusive ortholog search No orthologs identified
Ortholog(s) in Drosophila melanogaster (None identified)
No D. melanogaster orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (0) based on analysis using Dmel annotation version 4.3
No orthologs identified
Stocks & Reagents
Stocks Listed in FlyBase ( 66 )
Bloomington	40161 y1 M{vas-int.Dm}ZH-2A w* 23649 P{hsp70-flp}1, y1 w*; M{vas-int.B}ZH-102D 25709 y1 v1 P{nos-phiC31\int.NLS}X; P{CaryP}attP40 24483 y1 M{vas-int.Dm}ZH-2A w*; M{3xP3-RFP.attP}ZH-51D
Kyoto	130427 P{hsp70-flp}1, y1 w*; M{vas-int.B}ZH-102D 130431 y1 M{vas-int.Dm}ZH-2A w*; M{3xP3-RFP.attP}ZH-51D 130428 y1 M{3xP3-RFP.attP}ZH-2A w*; M{vas-int.Dm}ZH-102D 130429 y1 M{vas-int.Dm}ZH-2A w*; M{3xP3-RFP.attP'}ZH-22A 130430 y1 M{vas-int.Dm}ZH-2A w*; M{3xP3-RFP.attP'}ZH-51C 130432 y1 M{vas-int.Dm}ZH-2A w*; M{3xP3-RFP.attP'}ZH-58A 130433 y1 M{vas-int.Dm}ZH-2A w*; M{3xP3-RFP.attP'}ZH-68E 130435 y1 M{vas-int.Dm}ZH-2A w*; M{3xP3-RFP.attP'}ZH-96E
	More stocks available...
Genomic Clones ( 0 )
cDNA Clones ( 0 )
cDNA Clones, Fully Sequenced
BDGP DGC clones
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones
RNAi & Array Information
Linkouts
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	phiC31\int functions efficiently as a site-specific integrase (mediating recombination between the phiC31\attP and Sliv\attB recognition sites) in D.melanogaster in cultured cells and embryos. (Groth et al., 2004) phiC31\int encodes a site-specific integrase that mediates unidirectional site-specific recombination between the phiC31\attP recognition site encoded by the phiC31 genome and the attB recognition site encoded by the host chromosome (for example Sliv\attB).
Tag or Foreign Gene Data
	Foreign sequence; species == bacteriophage phi-C31; gene == 'int'; UniProt:Q9T221.
External Crossreferences & Linkouts
Sequence Crossreferences
Other Crossreferences
Linkouts
Synonyms & Secondary IDs ( 10 )
Reported As
Symbol Synonym	int (Bischof and Basler, 2007.11.7) phiC31 (Bischof et al., 2007) phiC31\int   ΠC31 (Choi et al., 2009) ΦC31 (Huang et al., 2011)
Name Synonym	bacteriophage phi-C31 integrase   integrase (Bateman et al., 2006, Bischof et al., 2007) PhiC31 integrase (Boy et al., 2010) phiC31 integrase (Kadowaki, 2011.10.7) φC31-integrase (Venken et al., 2006)
Secondary FlyBase IDs
References ( 13 )
Research paper	Huang et al., 2011, Genetics 189(1): 391--395 Successive and Targeted DNA Integrations in the Drosophila Genome by Bxb1 and {varphi}C31 Integrases. [FBrf0215219] Boy et al., 2010, genesis 48(7): 452--456 Vectors for efficient and high-throughput construction of fluorescent drosophila reporters using the PhiC31 site-specific integration system. [FBrf0211275] Choi et al., 2009, Science 324(5923): 54 Conditional mutagenesis in Drosophila. [FBrf0207727] Griffin et al., 2009, Nat. Methods 6(8): 600--602 The twin spot generator for differential Drosophila lineage analysis. [FBrf0208347] Huang et al., 2009, Proc. Natl. Acad. Sci. U.S.A. 106(20): 8284--8289 Directed, efficient, and versatile modifications of the Drosophila genome by genomic engineering. [FBrf0208027] Bischof et al., 2007, Proc. Natl. Acad. Sci. U.S.A. 104(9): 3312--3317 An optimized transgenesis system for Drosophila using germ-line-specific φC31 integrases. [FBrf0192974] Bateman et al., 2006, Genetics 173(2): 769--777 Site-specific transformation of Drosophila via phiC31 integrase-mediated cassette exchange. [FBrf0194832] Venken et al., 2006, Science 314(5806): 1747--1751 P[acman]: a BAC transgenic platform for targeted insertion of large DNA fragments in D. melanogaster. [FBrf0194996] Groth et al., 2004, Genetics 166(4): 1775--1782 Construction of Transgenic Drosophila by Using the Site-Specific Integrase From Phage {phi}C31. [FBrf0174693]
Supplementary material	Bischof et al., 2007, Proc. Natl. Acad. Sci. U.S.A. 104(9): SI Text. [FBrf0198668]
Personal communication to FlyBase	Kadowaki, 2011.10.7, Kadowaki constructs and insertions. Kadowaki constructs and insertions. [FBrf0216690] Bischof and Basler, 2007.11.7, Information on M{vas-int.Dm} stocks. Information on M{vas-int.Dm} stocks. [FBrf0200341]
FlyBase analysis	FlyBase Curators, 1992-, Summary information describing the origin of tags and foreign genes in FlyBase. Summary information describing the origin of tags and foreign genes in FlyBase. [FBrf0199194]