FlyBase Gene Report: Dmel\side-VI

General Information

Symbol

Dmel\side-VI

Species

D. melanogaster

Name

sidestep VI

Annotation Symbol

CG34114

Feature Type

protein_coding_gene

FlyBase ID

FBgn0083950

Gene Model Status

Current

Stock Availability

9 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

Sequence location

3R:10,972,381..11,050,175 [+]

Recombination map

3-50

RefSeq locus

NT_033777 REGION:10972381..11050175

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (0 terms)

Molecular Function (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

SIDE FAMILY

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

SIDE FAMILY -: SIDE is a closely related family encoding immunoglobulin superfamily proteins. They can interact with BEAT family members. Interactions between side and beat-Ia have been shown to play a role in the control of motor axon guidance. (Adapted from FBrf0207934 and FBrf0222007).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\side-VI for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q0KI85)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.49

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

side-VI-RB

FBtr0110808

NM_001043240

6818

1087

side-VI-RC

FBtr0301070

NM_001170115

4318

760

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

side-VI-PB

FBpp0110105

118.8

1087

6.79

NP_001036705

ABI31162

side-VI-PC

FBpp0290292

84.3

760

6.54

NP_001163586

ACZ94883

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\side-VI using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG34114_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 16

embryonic/larval central nervous system

(Li et al., 2017)

muscle cell of A1-7 ventral longitudinal muscle 1

(Li et al., 2017)

muscle cell of A1-7 ventral longitudinal muscle 2

(Li et al., 2017)

abdominal intersegmental bidendritic neuron isbp

(Li et al., 2017)

dorsal median cell

(Li et al., 2017)

Additional Descriptive Data

side-VI is expressed at key choice points for motor axons in the intersegmental nerve, the ISNb nerves and transverse nerves. It is broadly expressed in the CNS and in subsets of muscle fibers including ventral longitudinal muscles 1 and 2. It is also expressed in other cells whose surfaces are explored by the ISN tip. It is expressed in targets of the transverse nerve known to be important for its guidance including the bidendritic neurons and the dorsal median cell.

(Li et al., 2017)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\side-VI in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\side-VI

View exonic expression by tissue for Dmel\side-VI

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0083950

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

4379854/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0083950

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0083950

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0083950

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4695

Flygut - An atlas of the Drosophila adult midgut

FBgn0083950

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 7 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of side-VI

Transgenic constructs containing regulatory region of side-VI

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene disrupted in

Df(3R)Exel7306

(Ryder, 2004.4.26)

Df(3R)TENGL4¹

(Deluca and O'Farrell, 2012)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

viable, with Scer\GAL4^Mef2.PR

viable, with Scer\GAL4^pnr-MD237

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (4)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\MCAM

1 of 15

Yes

Hsap\MERTK

1 of 15

Yes

Hsap\MIR6756

1 of 15

Yes

Hsap\NPHS1

1 of 15

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Nphs1

1 of 15

Yes

Rattus norvegicus (Norway rat) (1)

Rnor\Nphs1

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (2)

Xtro\LOC100216153

1 of 12

Yes

Xtro\nphs1

1 of 12

Yes

Danio rerio (Zebrafish) (10)

Drer\LOC566194

2 of 15

Yes

Drer\hmcn2

1 of 15

Drer\icam3

1 of 15

Yes

Drer\nphs1

1 of 15

Drer\sc:d0202

1 of 15

Yes

Drer\si:ch73-208g10.1

1 of 15

Yes

Drer\si:ch211-66e2.3

1 of 15

Yes

Drer\si:ch211-66e2.5

1 of 15

Yes

Drer\si:ch211-74m13.1

1 of 15

Yes

Drer\si:ch211-180a12.2

1 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (2)

Cele\igcm-1

1 of 15

Yes

Cele\syg-2

1 of 15

Yes

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (14)

Gene Symbol

Score

Source

Alignment

4 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

nrm

2 of 10

2 of 10

1 of 10

1 of 10

1 of 10

hbs

1 of 10

sns

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

side-VI - beat-Va

surface plasmon resonance, predetermined participant, affinity technology, tag visualisation by enzyme assay, enzyme linked immunosorbent assay

(Li et al., 2017, Ozkan et al., 2013)

side-VI - beat-Vb

affinity technology, tag visualisation by enzyme assay, enzyme linked immunosorbent assay, surface plasmon resonance, predetermined participant

(Li et al., 2017, Ozkan et al., 2013)

side-VI - beat-Vc

enzyme linked immunosorbent assay, surface plasmon resonance, predetermined participant

(Li et al., 2017, Ozkan et al., 2013)

side-VI - hig

enzyme linked immunosorbent assay

(Ozkan et al., 2013)

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

607083

DroID - A comprehensive database of gene and protein interactions.

FBgn0083950

MIST (protein-protein) - An integrated Molecular Interaction Database

CG34114

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-50

Cytogenetic map

86C15-86D1

Sequence location

3R:10,972,381..11,050,175 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

86C15-86D1

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-50

(FlyBase, 2016)

3-47.2

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (9)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

34145

y¹ w^*; Mi{MIC}side-VI^MI01052

38121

y¹ w^*; Mi{MIC}side-VI^MI03149

58672

y¹ w^*; Mi{MIC}side-VI^MI12999

61719

y¹ w^*; Mi{MIC}side-VI^MI13088

22809

y¹ w^67c23; Mi{ET1}side-VI^MB00468

60515

y¹ w^*; Mi{PT-GFSTF.1}side-VI^{MI01052-GFSTF.1}

82012

y¹ sc^* v¹ sev²¹; P{TKO.GS04406}attP40

VDRC

v103456

P{KK113375}VIE-260B

v38809

w¹¹¹⁸; P{GD8414}v38809

Genomic Clones (61)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (36)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0083950

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0083950

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

4379854

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: side-VI CG34114

(Li et al., 2017)

Source for database merge of

Source for merge of: CG14698 CG4695

(FlyBase Genome Annotators, 2006)

Additional comments

Annotations CG14698 and CG4695 merged as CG34114 in release 5.1 of the genome annotation.

(FlyBase Genome Annotators, 2006)

Other Comments

Origin and Etymology

Discoverer

Etymology

Named 'side-VI' based on its phylogenetic relationship to 'side'.

(Li et al., 2017)

Identification

External Crossreferences and Linkouts ( 45 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

4379854

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

AI257771

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q0KI85

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q0KI85

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0083950

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0083950

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

4379854/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0083950

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG34114_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0083950

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0083950

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4695

Flygut - An atlas of the Drosophila adult midgut

FBgn0083950

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

4379854

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG34114

MARRVEL_MODEL - MARRVEL (model organism gene)

4379854

modMine - A data warehouse for the modENCODE project

FBgn0083950

Linkouts

BioGRID - A database of protein and genetic interactions.

607083

DroID - A comprehensive database of gene and protein interactions.

FBgn0083950

DRSC - Results frm RNAi screens

FBgn0083950

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0083950

FlyMine - An integrated database for Drosophila genomics

FBgn0083950

MIST (protein-protein) - An integrated Molecular Interaction Database

CG34114

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

34114

(Ozkan et al., 2013)

CG14698

(Giot et al., 2003, Vogel et al., 2003)

CG34114

(Mukherjee et al., 2020, Li et al., 2017, Gene Disruption Project members, 2015-, Kern et al., 2015, Ashwal-Fluss et al., 2014, Ozkan et al., 2013, Ozkan et al., 2013, Keleman et al., 2009.8.5)

CG4695

(Giot et al., 2003)

side-VI

(Li et al., 2017, Transgenic RNAi Project members, 2017-)

Name Synonyms

sidestep VI

Secondary FlyBase IDs