FlyBase Gene Report: Dmel\Drgx

PAIRED-LIKE HOMEOBOX TRANSCRIPTION FACTORS -: Paired-like (PRD-like) homeobox transcription factors are sequence-specific DNA binding proteins that regulate transcription. PRD-like transcription factors lack a paired domain but possess a homeodomain characteristic of the paired homeobox transcription factors. Some members also possess an Engrailed Homology 1 (EH1) and/or an OAR motif near the C-terminus. (Adapted from FBrf0232555 and FBrf0106517).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\Drgx or the JBrowse view of Dmel\Drgx for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.49

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Drgx-RE

FBtr0302391

NM_001103605

2612

587

Drgx-RF

FBtr0339572

NM_001298663

2989

587

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Drgx-PE

FBpp0291586

61.5

587

7.41

NP_001097075

ABV53614

Drgx-PF

FBpp0308648

61.5

587

7.41

NP_001285592

AHN54107

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

587 aa isoforms: Drgx-PE, Drgx-PF

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

A0AVV3

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Drgx using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG34340_1

Linkouts

Gene Ontology (4 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

DNA-binding transcription factor activity, RNA polymerase II-specific

inferred from biological aspect of ancestor with PANTHER:PTN002518769

(assigned by GO_Central )

(Gaudet et al., 2011)

RNA polymerase II regulatory region sequence-specific DNA binding

inferred from biological aspect of ancestor with PANTHER:PTN002518769

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

regulation of transcription, DNA-templated

inferred from electronic annotation with InterPro:IPR017970

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

nucleus

inferred from biological aspect of ancestor with PANTHER:PTN002518769

(assigned by GO_Central )

(Gaudet et al., 2011)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

central brain primordium

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

ventral nerve cord

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Drgx in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Drgx

View exonic expression by tissue for Dmel\Drgx

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0085369

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0085369

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG2808-RB;HDC00631;CG10017-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG2808

Flygut - An atlas of the Drosophila adult midgut

FBgn0085369

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 7 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 7 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Drgx

Transgenic constructs containing regulatory region of Drgx

Deletions and Duplications ( 1 )

Disrupted in

Df(2L)Exel7018

(Ryder, 2004.4.26)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

cell lethal, with Scer\GAL4^ey.PU

Drgx^GLO

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Drgx^GD5038

viable, with Scer\GAL4^Mef2.PR

Drgx^GD5038

viable, with Scer\GAL4^pnr-MD237

Drgx^GD5038

Other Phenotypes

Allele

cell migration defective | somatic clone, with Scer\GAL4^Act5C.PP

Drgx^GLO

visible, with Scer\GAL4^Bx-MS1096

Drgx^{UAS.ORF.GW.Tag:HA}

visible, with Scer\GAL4^ey.PU

Drgx^GLO

visible, with Scer\GAL4^GMR.PU

Drgx^GLO

Phenotype manifest in

Allele

eye, with Scer\GAL4^ey.PU

Drgx^GLO

eye, with Scer\GAL4^GMR.PU

Drgx^GLO

follicle cell | somatic clone, with Scer\GAL4^Act5C.PP

Drgx^GLO

head, with Scer\GAL4^ey.PU

Drgx^GLO

wing, with Scer\GAL4^Bx-MS1096

Drgx^{UAS.ORF.GW.Tag:HA}

wing vein, with Scer\GAL4^Bx-MS1096

Drgx^GLO

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (9)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\DRGX

6 of 15

Yes

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (10)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Prrxl1

6 of 15

Yes

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Mmus\Rhox11

1 of 15

Yes

Mmus\Rhox12

1 of 15

Yes

Mmus\Rhox13

1 of 15

Yes

Rattus norvegicus (Norway rat) (10)

Rnor\Drgx

4 of 13

Yes

Rnor\Alx1

1 of 13

Rnor\Alx3

1 of 13

Yes

Rnor\Esx1

1 of 13

Yes

Rnor\LOC691272

1 of 13

Yes

Rnor\Phox2b

1 of 13

Yes

Rnor\Rhox11

1 of 13

Yes

Rnor\Rhox12

1 of 13

Yes

Rnor\Rhox13

1 of 13

Yes

Rnor\Rhoxf10

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (5)

Xtro\drgx

3 of 12

Yes

Xtro\phox2a

2 of 12

Xtro\phox2b

2 of 12

Xtro\alx1

1 of 12

Yes

Xtro\alx4

1 of 12

Yes

Danio rerio (Zebrafish) (10)

Drer\drgx

6 of 15

Yes

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Drer\dharma

1 of 15

Yes

Drer\phox2ba

1 of 15

Drer\sebox

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (5)

Cele\ceh-8

2 of 15

Yes

Cele\ceh-17

2 of 15

Yes

Cele\ceh-45

1 of 15

Cele\ceh-53

1 of 15

Yes

Cele\lim-7

1 of 15

Arabidopsis thaliana (thale-cress) (3)

Atha\AtHB23

1 of 9

Yes

Atha\HB-7

1 of 9

Yes

Atha\HB17

1 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (2)

Scer\LSB1

1 of 15

Yes

Scer\PIN3

1 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\SPBC119.05c

1 of 12

Yes

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190BMN )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Dmel\Drgx

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA25749

Drosophila persimilis

Dper\GL18665

Drosophila willistoni

Dwil\GK14643

Drosophila virilis

Dvir\GJ21114

Drosophila virilis

Dvir\GJ11355

Drosophila mojavensis

Dmoj\GI23866

Drosophila grimshawi

Dgri\GH13699

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091508R1 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17011055

Glossina morsitans

Tsetse fly

Gmor\GMOY007983

Glossina morsitans

Tsetse fly

Gmor\GMOY004722

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_09643

Mayetiola destructor

Hessian fly

Mdes\Mdes018808

Mayetiola destructor

Hessian fly

Mdes\Mdes015792

Mayetiola destructor

Hessian fly

Mdes\Mdes015806

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL018154

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL015234

Anopheles darlingi

American malaria mosquito

Adar\ADAC005280

Anopheles gambiae

Malaria mosquito

Agam\AGAP008868

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ005281

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0PED )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA007471

Danaus plexippus

Monarch butterfly

Dple\DPOGS204692-PA

Apis florea

Little honeybee

Aflo\4305474628

Apis mellifera

Western honey bee

Amel\GB51950

Apis mellifera

Western honey bee

Amel\GB49866

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP24853

Bombus terrestris

Buff-tailed bumblebee

Bter\15210013

Linepithema humile

Argentine ant

Lhum\LH17469

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:001860

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG004827

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_05536

Tribolium castaneum

Red flour beetle

Tcas\TC005600

Pediculus humanus

Human body louse

Phum\PHUM189970

Rhodnius prolixus

Kissing bug

Rpro\RPRC006801

Cimex lectularius

Bed bug

Clec\CLEC001950

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI007256

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_13784

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0PPC )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR003736

Ixodes scapularis

Black-legged tick

Isca\ISCW021207

Tetranychus urticae

Two-spotted spider mite

Turt\tetur14g00760

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0YX9 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Arxl

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Phox2

Ciona intestinalis

Vase tunicate

Cint\Phox2

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000026017

Gallus gallus

Domestic chicken

Ggal\PHOX2B

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (10)

Gene Symbol

Score

Source

Alignment

3 of 10

2 of 10

CG9876

2 of 10

CG11294

2 of 10

hbn

2 of 10

Pph13

2 of 10

repo

2 of 10

CG32532

1 of 10

Gsc

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

DRGX; dorsal root ganglia homeobox

6 of 15

606701

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Drgx - REPTOR-BP

two hybrid array

(Shokri et al., 2019)

Drgx - abd-A

bimolecular fluorescence complementation, fluorescence microscopy

(Bischof et al., 2018)

Drgx - l(1)sc

two hybrid array

(Shokri et al., 2019)

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

624807

DroID - A comprehensive database of gene and protein interactions.

FBgn0085369

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

5740176

Pathways

Gene Group - Pathway Membership (FlyBase)

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-10

Cytogenetic map

24A5-24B1

Sequence location

2L:3,662,745..3,692,114 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

24A5-24B1

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-10

(FlyBase, 2016)

2-8.9

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (13)

Bloomington

38045

y¹ w^*; Mi{MIC}Drgx^MI04684

42422

y¹ w^*; Mi{MIC}Drgx^MI06689

51138

y¹ w^*; Mi{MIC}Drgx^MI08993

56323

y¹ w^*; Mi{MIC}Drgx^MI11472/SM6a

56546

w^*; Mi{Hto-WP}Drgx^GLO Sfp24Bd^GLO

81192

y¹ w^*; TI{CRIMIC.TG4.1}Drgx^{CR01147-TG4.1}/SM6a

80268

y¹ sc^* v¹ sev²¹; P{TOE.GS02838}attP40

FlyORF

F004839

M{UAS-Drgx.ORF-VN}ZH-86Fb

F001861

M{UAS-Drgx.ORF.3xHA.GW}ZH-86Fb

c00498

v109018

v30703

w¹¹¹⁸; P{GD5038}v30703

More stocks available...

Genomic Clones (18)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (13)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

IP09201

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0085369

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0085369

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

5740176

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Drgx CG34340

(Javeed et al., 2015)

Source for database merge of

Source for merge of: CG10017 CG2808

(FlyBase Genome Annotators, 2007)

Additional comments

Annotations CG10017 and CG2808 merged as CG34340 in release 5.2 of the genome annotation.

(FlyBase Genome Annotators, 2007)

Other Comments

dsRNA has been made from templates generated with primers directed against this gene. RNAi of CG2808 results in reduced arborization of ddaD and ddaE neurons, defects in muscle and defects in dendrite morphogenesis.

(Parrish et al., 2006)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 28 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

5740176

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

BI584585

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information