FlyBase Gene Report: Dmel\CG34354

General Information

Symbol

Dmel\CG34354

Species

D. melanogaster

Name

Annotation Symbol

CG34354

Feature Type

protein_coding_gene

FlyBase ID

FBgn0085383

Gene Model Status

Current

Stock Availability

7 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

98C1-98C1

Sequence location

3R:28,146,880..28,240,526 [+]

Recombination map

3-95

RefSeq locus

NT_033777 REGION:28146880..28240526

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (3 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables mRNA binding

inferred from sequence or structural similarity

(Lasko, 2000)

inferred from sequence or structural similarity with UniProtKB:P32588

(FlyBase Curators, 2008-)

Biological Process (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in regulation of mRNA processing

inferred from sequence or structural similarity with UniProtKB:P31483

(FlyBase Curators, 2008-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

located_in nucleus

inferred from sequence or structural similarity with UniProtKB:P32588

(FlyBase Curators, 2008-)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\CG34354 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry A8JPX0)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Double stop-codon suppression (UAG, UGA) postulated; FBrf0222074, FlyBase analysis); supported by conservation of the extended ORF across Drosophila species (FBrf0202906).

Gene model reviewed during 5.53

Gene model reviewed during 6.02

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Sequence Ontology: Class of Gene

gene_with_edited_transcript

(Rodriguez et al., 2012)

gene_with_stop_codon_read_through

(FlyBase Genome Annotators, 2013)

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

CG34354-RD

FBtr0304917

NM_001170284

3135

431

CG34354-RE

FBtr0304916

NM_001170285

4568

431

CG34354-RF

FBtr0344296

NM_001104483

3313

550

CG34354-RG

FBtr0346762

NM_001316621

3313

434

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

CG34354-PD

FBpp0293456

46.1

431

7.23

NP_001163755

ACZ95049

CG34354-PE

FBpp0293455

46.1

431

7.23

NP_001163756

ACZ95050

CG34354-PF

FBpp0310700

60.4

550

6.90

NP_001097953

ABW08789

CG34354-PG

FBpp0312357

46.3

434

7.23

NP_001303550

ALI30647

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

431 aa isoforms: CG34354-PD, CG34354-PE

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CG34354 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG34354_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 5 of adulthood & male -- day 7 of adulthood & male

spermatozoon

(Wasbrough et al., 2010)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\CG34354 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\CG34354

View exonic expression by tissue for Dmel\CG34354

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0085383

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

5740528/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0085383

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0085383

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0085383

Flygut - An atlas of the Drosophila adult midgut

FBgn0085383

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 10 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 7 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of CG34354

Transgenic constructs containing regulatory region of CG34354

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

CG34354^GD11426

Other Phenotypes

Allele

some die during pupal stage, with Scer\GAL4^pnr-MD237

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (41)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\TIA1

7 of 15

Yes

Hsap\TIAL1

7 of 15

Yes

Hsap\BOLL

1 of 15

Hsap\CIRBP

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Hsap\RBM3

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (42)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Tial1

7 of 15

Yes

Mmus\Tia1

6 of 15

Mmus\Boll

1 of 15

Mmus\Cirbp

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Rattus norvegicus (Norway rat) (38)

Rnor\Tial1

7 of 13

Yes

Rnor\Tia1

6 of 13

Rnor\Boll

1 of 13

Rnor\Cirbp

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (35)

Xtro\tia1

6 of 12

Yes

Xtro\tial1

2 of 12

Yes

Xtro\cirbp

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Danio rerio (Zebrafish) (46)

Drer\tia1

7 of 15

Yes

Drer\tia1l

7 of 15

Yes

Drer\tial1

7 of 15

Yes

Drer\cirbpa

1 of 15

Yes

Drer\cirbpb

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Drer\zgc:55733

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (17)

Cele\tiar-2

7 of 15

Yes

Cele\tiar-1

6 of 15

Cele\tiar-3

5 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Arabidopsis thaliana (thale-cress) (9)

Atha\AT3G14100

5 of 9

Yes

Atha\UBP1A

5 of 9

Yes

Atha\UBP1B

5 of 9

Yes

Atha\AT2G21440

1 of 9

Atha\AT2G41060

1 of 9

Yes

Atha\AT3G09160

1 of 9

Yes

Atha\EIF3G1

1 of 9

Atha\EIF3G2

1 of 9

Atha\UBA2A

1 of 9

Saccharomyces cerevisiae (Brewer's yeast) (23)

Scer\PUB1

7 of 15

Yes

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Scer\MUD1

1 of 15

Scer\NAB3

1 of 15

Scer\NAB6

1 of 15

Yes

Scer\NOP6

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Scer\RNA15

1 of 15

Scer\RRT5

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

Schizosaccharomyces pombe (Fission yeast) (3)

Spom\csx1

1 of 12

Yes

Spom\SPBC23E6.01c

1 of 12

Yes

Spom\SPBC660.15

1 of 12

Yes

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (44)

Gene Symbol

Score

Source

Alignment

5 of 10

trv

4 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

B52

1 of 10

bol

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

fne

1 of 10

Hrb27C

1 of 10

Larp7

1 of 10

maca

1 of 10

msi

1 of 10

Rbp6

1 of 10

Rbp9

1 of 10

rump

1 of 10

SF2

1 of 10

snRNP-U1-70K

1 of 10

sqd

1 of 10

ssx

1 of 10

Sxl

1 of 10

Syp

1 of 10

tra2

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

TIA1; TIA1 cytotoxic granule associated RNA binding protein

7 of 15

603518

TIAL1; TIA1 cytotoxic granule associated RNA binding protein like 1

7 of 15

603413

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

625159

DroID - A comprehensive database of gene and protein interactions.

FBgn0085383

MIST (protein-protein) - An integrated Molecular Interaction Database

CG34354

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0085383

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-95

Cytogenetic map

98C1-98C1

Sequence location

3R:28,146,880..28,240,526 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

98C1-98C1

Limits computationally determined from genome sequence between P{PZ}btz^rL203 and P{PZ}l(3)06487⁰⁶⁴⁸⁷

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-95

(FlyBase, 2016)

3-93.2

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (7)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

27301

y¹ v¹; P{TRiP.JF02620}attP2

25644

w¹¹¹⁸; Mi{ET1}CG34354^MB07567

59175

y¹ w^*; Mi{MIC}CG34354^MI13643

23019

y¹ w^67c23; Mi{ET1}CG34354^MB01203

33674

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00538}attP2

VDRC

v102597

P{KK112134}VIE-260B

v21949

w¹¹¹⁸; P{GD11426}v21949/TM3

Genomic Clones (65)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (80)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

SD09246

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0085383

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

5740528

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: CG12425 CG12852 CG4787

(FlyBase Genome Annotators, 2007)

Additional comments

Annotations CG12425, CG12852, and CG4787 merged as CG34354 in release 5.2 of the genome annotation.

(FlyBase Genome Annotators, 2007)

"CG12425" may correspond to "Scim37". Sequence analysis off ends of P{SUPor-P} in Scim insertion mutant places "Scim37" near/in "CG12425".

(Dobie and Karpen, 2001.4.18)

Other Comments

RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 41 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

5740528

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

A8JPX0

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

A8JPX0

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0085383

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

5740528/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0085383

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG34354_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0085383

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0085383

Flygut - An atlas of the Drosophila adult midgut

FBgn0085383

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0085383

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

5740528

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG34354

MARRVEL_MODEL - MARRVEL (model organism gene)

5740528

modMine - A data warehouse for the modENCODE project

FBgn0085383

Linkouts

BioGRID - A database of protein and genetic interactions.

625159

DroID - A comprehensive database of gene and protein interactions.

FBgn0085383

DRSC - Results frm RNAi screens

FBgn0085383

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0085383

FlyMine - An integrated database for Drosophila genomics

FBgn0085383

MIST (protein-protein) - An integrated Molecular Interaction Database

CG34354

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

CG12425

(Stanyon et al., 2004)

CG12852

(Hauenschild et al., 2008)

CG34354

(Kurmangaliyev et al., 2020, Meltzer et al., 2019, Hosono et al., 2015, Kern et al., 2015, Ashwal-Fluss et al., 2014, FlyBase Genome Annotators, 2013, Terriente-Felix et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Rodriguez et al., 2012, Ni et al., 2010.12.1, Wasbrough et al., 2010, Keleman et al., 2009.8.5, Perkins et al., 2009.8.10, FlyBase Genome Annotators, 2007)

CG4787

(Konieczny and Artero, 2020)

cg4787

(Lasko, 2000)

Name Synonyms

CG12425

CG12852

CG4787

Secondary FlyBase IDs