FlyBase Gene Report: Dmel\TrissinR

CLASS A GPCR NEUROPEPTIDE AND PROTEIN HORMONE RECEPTORS -: The majority of neuropeptide/protein hormone GPCR belong to the Class A family of GPCRs. The secreted ligands for this subfamily include neuropeptides, peptide hormones and protein hormones. (Adapted from FBrf0195077).

Protein Function (UniProtKB)

G-protein coupled receptor which is activated by the Trissin peptide in vitro, leading to increased intracellular calcium ion levels.

(UniProt, Q9VML9)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\TrissinR or the JBrowse view of Dmel\TrissinR for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.44

Gene model reviewed during 5.51

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

TrissinR-RB

FBtr0112619

NM_001103622

2826

669

TrissinR-RC

FBtr0330221

NM_135118

2811

664

TrissinR-RD

FBtr0330222

NM_001273179

3637

659

TrissinR-RE

FBtr0342612

NM_001298712

3601

664

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

TrissinR-PB

FBpp0111531

74.5

669

9.37

NP_001097092

AAF52294

TrissinR-PC

FBpp0303254

73.9

664

9.44

NP_608962

AAF52292

TrissinR-PD

FBpp0303255

73.4

659

9.52

NP_001260108

AGB92644

TrissinR-PE

FBpp0309550

73.9

664

9.44

NP_001285641

AHN54156

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

664 aa isoforms: TrissinR-PC, TrissinR-PE

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\TrissinR using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

TrissinR_1

Linkouts

Gene Ontology (8 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

G protein-coupled peptide receptor activity

inferred from physical interaction with FLYBASE:Trissin; FB:FBgn0038343

(Ida et al., 2011)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

G protein-coupled peptide receptor activity

inferred from sequence or structural similarity with FLYBASE:TkR99D; FB:FBgn0004622

(Brody and Cravchik, 2000)

G protein-coupled receptor activity

inferred from biological aspect of ancestor with PANTHER:PTN001202348

(assigned by GO_Central )

(Gaudet et al., 2011)

neuropeptide receptor activity

inferred from sequence model

(Hewes and Taghert, 2001)

peptide binding

inferred from biological aspect of ancestor with PANTHER:PTN001202348

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

G protein-coupled receptor signaling pathway

inferred from direct assay

(Ida et al., 2011)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

cellular response to hormone stimulus

inferred from biological aspect of ancestor with PANTHER:PTN001202348

(assigned by GO_Central )

(Gaudet et al., 2011)

G protein-coupled receptor signaling pathway

inferred from biological aspect of ancestor with PANTHER:PTN001202348

(assigned by GO_Central )

(Gaudet et al., 2011)

inferred from sequence model

(Hewes and Taghert, 2001)

inferred from sequence or structural similarity with FLYBASE:TkR99D; FB:FBgn0004622

(Brody and Cravchik, 2000)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

integral component of plasma membrane

inferred from direct assay

(Ida et al., 2011)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

integral component of membrane

inferred from sequence model

(Hewes and Taghert, 2001)

inferred from sequence or structural similarity with FLYBASE:TkR99D; FB:FBgn0004622

(Brody and Cravchik, 2000)

integral component of plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN001202348

(assigned by GO_Central )

(Gaudet et al., 2011)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

integral component of plasma membrane

inferred from direct assay

(Ida et al., 2011)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\TrissinR in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\TrissinR

View exonic expression by tissue for Dmel\TrissinR

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0085410

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG31645-RA;CG14004-RA

Flygut - An atlas of the Drosophila adult midgut

FBgn0085410

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 6 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of TrissinR

Transgenic constructs containing regulatory region of TrissinR

Deletions and Duplications ( 2 )

Disrupted in

Df(2L)Exel6014

(Ryder, 2004.4.26)

Df(2L)rau^Δ

(Sieglitz et al., 2013)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

TrissinR^MI05488

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

viable, with Scer\GAL4^Mef2.PR

viable, with Scer\GAL4^pnr-MD237

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (8)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\FFAR4

1 of 15

Yes

Hsap\GPR19

1 of 15

Yes

Hsap\GPR22

1 of 15

Yes

Hsap\HCRTR1

1 of 15

Yes

Hsap\HCRTR2

1 of 15

Yes

Hsap\NPBWR1

1 of 15

Yes

Hsap\PROKR1

1 of 15

Yes

Hsap\PROKR2

1 of 15

Yes

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (8)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Ffar4

1 of 15

Yes

Mmus\Gpr19

1 of 15

Yes

Mmus\Gpr22

1 of 15

Yes

Mmus\Hcrtr1

1 of 15

Yes

Mmus\Hcrtr2

1 of 15

Yes

Mmus\Oprd1

1 of 15

Yes

Mmus\Prokr1

1 of 15

Yes

Mmus\Prokr2

1 of 15

Yes

Rattus norvegicus (Norway rat) (7)

Rnor\Ffar4

1 of 13

Yes

Rnor\Gpr19

1 of 13

Yes

Rnor\Gpr22

1 of 13

Yes

Rnor\Hcrtr1

1 of 13

Yes

Rnor\Hcrtr2

1 of 13

Yes

Rnor\Prokr1

1 of 13

Yes

Rnor\Prokr2

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (1)

Xtro\gpr19

1 of 12

Yes

Danio rerio (Zebrafish) (8)

Drer\gpr19

1 of 15

Yes

Drer\gpr22a

1 of 15

Yes

Drer\gpr22b

1 of 15

Yes

Drer\hcrtr2

1 of 15

Yes

Drer\npffr2a

1 of 15

Yes

Drer\prokr1a

1 of 15

Yes

Drer\prokr1b

1 of 15

Yes

Drer\zmp:0000001069

1 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (4)

Cele\npr-20

7 of 15

Yes

Cele\npr-21

6 of 15

Yes

Cele\npr-3

1 of 15

Yes

Cele\T25F10.1

1 of 15

Yes

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091907C2 )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Dmel\TrissinR

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA16362

Drosophila persimilis

Dper\GL19038

Drosophila willistoni

Dwil\GK14702

Drosophila virilis

Dvir\GJ12041

Drosophila mojavensis

Dmoj\GI13390

Drosophila grimshawi

Dgri\GH10928

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091503CS )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17027083

Musca domestica

House fly

Mdoa\17008915

Glossina morsitans

Tsetse fly

Gmor\GMOY010450

Mayetiola destructor

Hessian fly

Mdes\Mdes006938

Aedes aegypti

Yellow fever mosquito

Aaeg\GPRNPR2

Anopheles darlingi

American malaria mosquito

Adar\ADAC005761

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ001963

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ001962

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W05WP )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\Bmo.12540

Danaus plexippus

Monarch butterfly

Dple\DPOGS215822-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL011637

Heliconius melpomene

Postman butterfly

Hmel\HMEL011636

Apis florea

Little honeybee

Aflo\2147602451

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP15437

Bombus terrestris

Buff-tailed bumblebee

Bter\15209321

Linepithema humile

Argentine ant

Lhum\LH11316

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:000957

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_05821

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_05820

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_02059

Tribolium castaneum

Red flour beetle

Tcas\TC014731

Pediculus humanus

Human body louse

Phum\PHUM618870

Pediculus humanus

Human body louse

Phum\PHUM447490

Pediculus humanus

Human body louse

Phum\PHUM278660

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_17704

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X05SE )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Ixodes scapularis

Black-legged tick

Isca\ISCW009718

Ixodes scapularis

Black-legged tick

Isca\ISCW006418

Tetranychus urticae

Two-spotted spider mite

Turt\tetur05g03320

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G08VN )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-QrfprL_4

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Hypp_1132

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (7)

Gene Symbol

Score

Source

Alignment

2 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

TrissinR - Trissin

ion exchange chromatography, molecular sieving, Identification by mass spectrometry, inferred by author

(Ida et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

59979

DroID - A comprehensive database of gene and protein interactions.

FBgn0085410

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

33812

MIST (protein-protein) - An integrated Molecular Interaction Database

TrissinR

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-17

Cytogenetic map

25F5-26A1

Sequence location

2L:5,824,004..5,856,767 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

25F5-26A1

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-17

(FlyBase, 2016)

2-19.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (12)

Bloomington

84585

w^*; TI{TI}TrissinR^attP

23428

w¹¹¹⁸; Mi{ET1}TrissinR^MB02243

42425

y¹ w^*; Mi{MIC}TrissinR^MI06711

60783

y¹ w^*; Mi{MIC}TrissinR^MI05488

36825

y¹ sc^* v¹ sev²¹; P{TRiP.GL01058}attP2

60109

y¹ sc^* v¹ sev²¹; P{TRiP.HMC05103}attP40

61782

y¹ w^67c23; Mi{PT-GFSTF.2}TrissinR^{MI05488-GFSTF.2}/SM6a

VDRC

v107943

P{KK107157}VIE-260B

v7886

w¹¹¹⁸; P{GD927}v7886

v42758

w¹¹¹⁸; P{GD928}v42758

v7885

w¹¹¹⁸; P{GD927}v7885/CyO

v42759

w¹¹¹⁸; P{GD928}v42759/CyO

Genomic Clones (29)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (122)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

IP06467

Other clones

IP12396

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0085410

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0085410

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

33812

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: TrissinR CG34381

(Ida et al., 2011)

Source for database merge of

Source for merge of: CG14004 CG31645

(FlyBase Genome Annotators, 2007)

Source for merge of: CG31645 anon-WO0170980.172 anon-WO0170980.173

(FlyBase, 1996-)

Source for merge of: CG14002 CG14003

(FlyBase Genome Annotators, 2002-2003)

Additional comments

Annotations CG14004 and CG31645 merged as CG34381 in release 5.2 of the genome annotation.

(FlyBase Genome Annotators, 2007)

Annotations CG14002 and CG14003 merged as CG31645 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Source for merge of CG31645 anon-WO0170980.172 anon-WO0170980.173 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Other Comments

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 36 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

33812

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VML9

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0085410

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

TrissinR_1

Flygut - An atlas of the Drosophila adult midgut

FBgn0085410

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

33812

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC014731

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG34381

modMine - A data warehouse for the modENCODE project

FBgn0085410

Linkouts

BioGRID - A database of protein and genetic interactions.

59979

DroID - A comprehensive database of gene and protein interactions.

FBgn0085410

DRSC - Results frm RNAi screens

FBgn0085410

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0085410

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG31645-RA;CG14004-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0085410

FlyMine - An integrated database for Drosophila genomics

FBgn0085410

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

33812

MIST (protein-protein) - An integrated Molecular Interaction Database

TrissinR

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

CG14002

CG14003

(Hauser et al., 2006, Hauser et al., 2006, Radford et al., 2002, Hewes and Taghert, 2001, Hewes and Taghert, 2001.7.31)

CG14004

CG31645

CG34381

(Hosono et al., 2015, Ashwal-Fluss et al., 2014, Cardoso et al., 2012, Cherbas et al., 2011, Ida et al., 2011, Jungreis, 2011.11.18, Keleman et al., 2009.8.5, Perkins et al., 2009, FlyBase Genome Annotators, 2007)

TrissinR

(Rao and Deng, 2019.10.23, Gene Disruption Project members, 2015-, Caers et al., 2012, Ni et al., 2011.7.19)

anon-WO0170980.172

anon-WO0170980.173

Name Synonyms

CG14004

CG31645

Trissin receptor

(Faisal et al., 2014, Caers et al., 2012)

trissin receptor

(Ida et al., 2011)

Secondary FlyBase IDs

FBgn0046569
FBgn0031744
FBgn0031743
FBgn0046570
FBgn0031742
FBgn0051645

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (45)

Report Sections

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