Pdm1, pdm-1, Pdm, dPOU-19, POU domain protein 1
transcription factor - homeodomain and pou domain - involved in cell fate determination of a subset of temporally determined neural subtypes - morphology ofsensory neurons is diversified through a series of suppressive transcriptional interactions involving Nubbin and Pdm2, Cut and transcription factors Scalloped and Vestigial - Nubbin and Teashirt mark barriers to clonal growth along the proximal-distal axis of the wing
Please see the JBrowse view of Dmel\nub for information on other features
To submit a correction to a gene model please use the Contact FlyBase form
Gene model reviewed during 5.51
3.3, 2.9, 2.8 (northern blot)
3.1 (northern blot)
4.9, 3.1, 2.6 (northern blot)
601 (aa); 65 (kD)
nub protein was shown to be a weak octamer-dependent activator of transcription when expressed in yeast and is thought to require additional coactivators to function efficiently.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\nub using the Feature Mapper tool.
Comment: reference states >=6 hr AEL
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reference states 0-6 hr AEL
Comment: reference states 0-12 hr AEL
Expression assayed at stages 9, 11, 13, and 17. Expression may be continuous between assayed stages in some tissues.
expressed first in SOP of this lineage expression subsequently decreases in presumptive glial cell, is re-established once gcm expression has been established, and then disappears by stage 16
Comment: area that corresponds to distal region of wing
nub marks the wing pouch and dorsal hinge.
Early in third instar, nub is expressed in a broad region from the pretarsus through the presumptive tarsal segments, up to the distal part of the dac domain in presumptive tarsal segment 2. By late third instar, nub expression disappears from the intersegmental region of true segments and the pretarsal region, remaining only in the joint-forming cells.
Between 4 and 12 hr APF, nub expression is proximally adjacent to Ecol\lacZodd-rK111, which labels the proximal parts of all leg joints except those between tarsal subsegments.
Markers that uniquely identify the cells of the NB3-7 lineage were used to examine the serotonin expressing cell lineages.
Expression in procephalic neuroblasts stage 9-11: tritocerebrum - d1-6, v1, v5; deuterocerebrum - d1-8, v2-8; protocerebrum - ad1, ad2, ad4-9, ad12, ad15, ad17, av1, cd1-6, cd8-14, cd17, cd20, cd21, cv1, cv3-9, pd2, pd4, pd5, pd8, pd9, pd12, pd16, pv1-3
Protein is detected in ring pattern in proximal joint of third instar larval leg discs. At 34-38 hours after pupal formation protein is expressed in the tibial and not the tarsal leg joints in the proximal joint tissue and a subset of bristle lineage.
nub protein is expressed in the wing pouch region of the third instar wing disc.
nub is expressed in the lateral, but not the medial, of the paired ventro-lateral serotonergic neurons.
GBrowse - Visual display of RNA-Seq signals
View Dmel\nub in GBrowse 22-47
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: nub CG6246
Source for merge of: nub CG15488 CG15489
Annotations CG6246, CG15489 and CG15488 merged as CG34395 in release 5.2 of the genome annotation.
The embryonic CNS contains sequentially generated neuroblast sublineages that can be distinguished by their expression of either hb, nub or cas. hb and cas may directly silence nub expression in early and late developing sublineages, given that nub cis-regulatory DNA contains approximately 32 hb/cas-binding sites and its enhancer(s) are ectopically activated in cas- neuroblasts. Targeted misexpression of cas in all neuronal lineages reduces nub expression without altering hb expression. By ensuring correct POU gene expression boundaries hb and cas maintain temporal subdivisions in the cell-identity circuitry controlling CNS development.
The POU domain protein encoded by nub contributes to the formation of a sharp dorsoventral boundary in the wing.
nub gene is important for correct proximal-distal specification of the growing wing imaginal disc.
Incorrect specification of the proximal-distal axis of the growing wing imaginal disc due to mutations in nub leads to failures in growth along both the D-V and A-P axis.
Isolated from a Drosophila 10-22 hour embryo cDNA expression library, using a 22bp sequence from the Cha upstream regulatory region as a probe. This 22bp sequence has been shown to be bound by specific binding factor(s) in embryo nuclear extracts.
nub and pdm2 both contribute toward the development of a full complement of mature RP2 neurons by being expressed in and acting on ganglion mother cell 2a (GMC-2a), the RP2 progenitor cell. nub plays a quantitatively less important role than pdm2. nub and pdm2 act to specify GMC-2a cell identity and not merely act to positively regulate GMC-2a cell division.
One of the homeodomain loci identified in a screen for genes encoding DNA binding proteins capable of binding to a consensus en binding site.
nub is transcribed in elements of the developing nervous system suggesting a functional role in neurogenesis and neurospecification.
nub contains a POU domain, and is expressed at maximum levels during early development.
Mickey, 10th May 1948.